pycom14g06450

AP2-like ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006364
Physical Location & Seq
Reverse (-)
6360205 .. 6363327
3123 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1377 bp
ATGAACAAAATCAAACGAAGGACTCACTCACTCATCTCTCTCTTTCTCTCTCTCTCTCTCCGCGGTTTTGGTTTGGGGGACTCAATTGGAGTGTTTAATCATATGGCGTCGTCGTCCTCGGATCCTGGTCTGAAGACCGAAAGCGGAAGCGGGGGCGGCAGTGGCGGTGGAGGAGCAGAGGCGTCGGAGGCGGTTATAGCGAGCGATCAGCTAATGGTGTACAGAGGGGTGAAGAAATCGAAGAAAGAGAGAGGATGTACGGCGAAAGAGCGCATCAGCAAAATGCCTCCGTGTGCTGCTGGAAAACGGAGCTCCATTTACCGTGGAGTCACTCGGCATAGATGGACAGGTCGTTATGAGGCTCATCTTTGGGATAAAAGTACCTGGAATCACAACCAGAATAAGAAGGGAAAGCAAGTTTACTTGGGGGCGTATGACGATGAAGAGGCTGCAGCTAGAGCTTATGATCTTGCTGCCTTGAAATACTGGGGTCCTGGCACTCTCATCAATTTTCCTGTTACTGATTACACAAGGGATCTTGAAGAGATGCAGAATGTGTCAAGGGAGGAATACCTTGCTTCTCTTCGAAGAAAGAGCAGTGGTTTCTCAAGAGGAATTTCCAAATTTCGTGGGCTTTCTAGTCGTTGGGAGCCGTCGTTGGGTCGTATGGGTGGATCTGAGTACTTCAATAGCACACATTATGGTGTTGATCCAGCAACAGAAAGTGATATTTTGGGAGCTCTTTGCATTGAAAGAAAGATTGACCTAACGAGTTACATCAAGTGGTGGCGATCCAACAGATCTCGACAAGCTGAAACTAGCATGAAATTTTCAGAAGAAACAAAACACGGTTGTGTTGGAGATATTGGAGTTGAACTTAAAACATTAGAATGGGGAATCCAGCCTACTGAACCATACCAGATGCCCCGTTTGGGCATCTCTCGTGTAAGTAAAAAACATAAAGGTACCAGAGTCTCTGCGATGAGCATCCTCTCACGGTCAGCTGCCTATAAGAACTTGCAAGAGAAAGCATCACAAAAGGAGGAAAACAATGCAGATAATGATGAGAATGAAAACAAAAATATCATCCACAAGATGGACTATGGAAAGGCAATTGAGAAATCCACAAGTCATGACGAAAGACTTAGTTCTGTATTAGGAATGAGTGGTGGAATGTCTCTCCAAACAGATGTGTTCCCGTTGGCTCCCTTCTTGTCTGCACCACTTCTGACCAACTACAATGCTGTTGATCCCTTGGTAGACCCCATTCTTTGGACGTCTCTTGCTCCTGTTCTTCCTTCTGGAATTTCTCGCACGACTGAGATTACAAAGACAGAGACAAGTTCAACTTTCACTTTATTTCAGCCCGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AP2

Protein Analysis

459

Amino Acids

50.78

Weight (kDa)

9.19

Isoelectric Point (pI)

54.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 107 - 164 7.2e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1280
Acc65I GGTACC 1 cut(s) 965
AccB1I GGYRCC 1 cut(s) 965
AccB7I CCANNNNNTGG 2 cut(s) 1096, 1272
AccI GTMKAC 1 cut(s) 1260
AccII CGCG 1 cut(s) 63
AciI CCGC 7 cut(s) 61, 63, 144, 150, 156, 165, 191
AclWI GGATC 7 cut(s) 116, 129, 543, 682, 704, 786, 1244
AcsI RAATTY 4 cut(s) 615, 623, 827, 1305
AcuI CTGAAG 1 cut(s) 152
AcyI GRCGYC 3 cut(s) 107, 182, 1277
AfaI GTAC 5 cut(s) 221, 259, 382, 683, 967
AfiI CCNNNNNNNGG 4 cut(s) 659, 932, 1096, 1272
AgsI TTSAA 6 cut(s) 481, 542, 688, 752, 875, 1347
AjnI CCWGG 3 cut(s) 124, 383, 493
AleI CACNNNNGTG 1 cut(s) 852
AluBI AGCT 7 cut(s) 211, 312, 455, 461, 740, 812, 1004
AluI AGCT 7 cut(s) 211, 312, 455, 461, 740, 812, 1004
Alw21I GWGCWC 2 cut(s) 314, 742
Alw26I GTCTC 4 cut(s) 979, 1182, 1284, 1331
AlwI GGATC 7 cut(s) 116, 129, 543, 682, 704, 786, 1244
ApeKI GCWGC 5 cut(s) 296, 449, 452, 473, 1004
ApoI RAATTY 4 cut(s) 615, 623, 827, 1305
Asp718I GGTACC 1 cut(s) 965
AspLEI GCGC 1 cut(s) 273
AspS9I GGNCC 1 cut(s) 491
AsuHPI GGTGA 1 cut(s) 241
AsuII TTCGAA 1 cut(s) 586
AvaII GGWCC 1 cut(s) 491
BaeI ACNNNNGTAYC 2 cut(s) 957, 990
BamHI GGATCC 1 cut(s) 121
BanI GGYRCC 1 cut(s) 965
BanII GRGCYC 2 cut(s) 314, 742
BauI CACGAG 1 cut(s) 942
BbsI GAAGAC 1 cut(s) 140
Bbv12I GWGCWC 2 cut(s) 314, 742
BbvI GCAGC 5 cut(s) 283, 436, 460, 464, 991
BccI CCATC 2 cut(s) 336, 1090
BceAI ACGGC 2 cut(s) 276, 637
BcgI CGANNNNNNTGC 2 cut(s) 165, 199
BciT130I CCWGG 3 cut(s) 126, 385, 495
BcoDI GTCTC 4 cut(s) 979, 1182, 1284, 1331
BfaI CTAG 3 cut(s) 456, 639, 819
BfmI CTRYAG 1 cut(s) 450
BglII AGATCT 1 cut(s) 800
BisI GCNGC 6 cut(s) 157, 297, 450, 453, 474, 1005
BlsI GCNGC 6 cut(s) 158, 298, 451, 454, 475, 1006
BmcAI AGTACT 1 cut(s) 683
Bme1390I CCNGG 3 cut(s) 126, 385, 495
Bme18I GGWCC 1 cut(s) 491
BmgT120I GGNCC 1 cut(s) 491
BmiI GGNNCC 5 cut(s) 123, 492, 651, 967, 1206
BmrFI CCNGG 3 cut(s) 126, 385, 495
BmrI ACTGGG 1 cut(s) 496
BmsI GCATC 6 cut(s) 282, 537, 912, 945, 996, 1040
BmuI ACTGGG 1 cut(s) 496
BpiI GAAGAC 1 cut(s) 140
Bpu14I TTCGAA 1 cut(s) 586
BpuEI CTTGAG 1 cut(s) 592
BsaBI GATNNNNATC 1 cut(s) 986
BsaHI GRCGYC 3 cut(s) 107, 182, 1277
BsaJI CCNNGG 4 cut(s) 61, 117, 322, 1254
BsaXI ACNNNNNCTCC 2 cut(s) 641, 671
Bsc4I CCNNNNNNNGG 4 cut(s) 659, 932, 1096, 1272
Bse1I ACTGG 1 cut(s) 491
Bse8I GATNNNNATC 1 cut(s) 986
BseBI CCWGG 3 cut(s) 126, 385, 495
BseDI CCNNGG 4 cut(s) 61, 117, 322, 1254
BseGI GGATG 3 cut(s) 260, 987, 1086
BseJI GATNNNNATC 1 cut(s) 986
BseLI CCNNNNNNNGG 4 cut(s) 659, 932, 1096, 1272
BseMII CTCAG 2 cut(s) 669, 1311
BseNI ACTGG 1 cut(s) 491
BseRI GAGGAG 1 cut(s) 186
BseXI GCAGC 5 cut(s) 283, 436, 460, 464, 991
BsgI GTGCAG 1 cut(s) 1203
Bsh1236I CGCG 1 cut(s) 63
BshNI GGYRCC 1 cut(s) 965
BsiHKAI GWGCWC 2 cut(s) 314, 742
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 4 cut(s) 659, 932, 1096, 1272
BsmAI GTCTC 4 cut(s) 979, 1182, 1284, 1331
BsmBI CGTCTC 1 cut(s) 1284
BsmFI GGGAC 1 cut(s) 92
Bsp119I TTCGAA 1 cut(s) 586
Bsp1286I GDGCHC 2 cut(s) 314, 742
Bsp1407I TGTACA 1 cut(s) 219
Bsp143I GATC 9 cut(s) 121, 205, 466, 535, 674, 709, 791, 800, 1249
BspACI CCGC 7 cut(s) 61, 63, 144, 150, 156, 165, 191
BspCNI CTCAG 2 cut(s) 670, 1312
BspFNI CGCG 1 cut(s) 63
BspHI TCATGA 1 cut(s) 1132
BspLI GGNNCC 5 cut(s) 123, 492, 651, 967, 1206
BspMAI CTGCAG 1 cut(s) 454
BspPI GGATC 7 cut(s) 116, 129, 543, 682, 704, 786, 1244
BspT104I TTCGAA 1 cut(s) 586
BspT107I GGYRCC 1 cut(s) 965
BsrGI TGTACA 1 cut(s) 219
BsrI ACTGG 1 cut(s) 491
BssECI CCNNGG 4 cut(s) 61, 117, 322, 1254
BssMI GATC 9 cut(s) 121, 205, 466, 535, 674, 709, 791, 800, 1249
BssNI GRCGYC 3 cut(s) 107, 182, 1277
BssSI CACGAG 1 cut(s) 942
BssT1I CCWWGG 1 cut(s) 1254
Bst2BI CACGAG 1 cut(s) 942
Bst2UI CCWGG 3 cut(s) 126, 385, 495
Bst4CI ACNGT 3 cut(s) 323, 851, 999
Bst6I CTCTTC 3 cut(s) 438, 537, 588
BstACI GRCGYC 3 cut(s) 107, 182, 1277
BstAUI TGTACA 1 cut(s) 219
BstBI TTCGAA 1 cut(s) 586
BstC8I GCNNGC 1 cut(s) 202
BstDEI CTNAG 3 cut(s) 678, 1145, 1320
BstDSI CCRYGG 2 cut(s) 61, 322
BstF5I GGATG 3 cut(s) 260, 987, 1086
BstFNI CGCG 1 cut(s) 63
BstHHI GCGC 1 cut(s) 273
BstKTI GATC 9 cut(s) 124, 208, 469, 538, 677, 712, 794, 803, 1252
BstMAI GTCTC 4 cut(s) 979, 1182, 1284, 1331
BstMBI GATC 9 cut(s) 121, 205, 466, 535, 674, 709, 791, 800, 1249
BstMWI GCNNNNNNNGC 5 cut(s) 156, 162, 188, 197, 458
BstNI CCWGG 3 cut(s) 126, 385, 495
BstSCI CCNGG 3 cut(s) 124, 383, 493
BstSFI CTRYAG 1 cut(s) 450
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 5 cut(s) 283, 436, 460, 464, 991
BstV2I GAAGAC 1 cut(s) 140
BstX2I RGATCY 4 cut(s) 121, 535, 674, 800
BstYI RGATCY 4 cut(s) 121, 535, 674, 800
BtgI CCRYGG 2 cut(s) 61, 322
BtgZI GCGATG 1 cut(s) 995
BtsCI GGATG 3 cut(s) 260, 987, 1086
BtsI GCAGTG 2 cut(s) 166, 604
BtsIMutI CAGTG 2 cut(s) 166, 604
Cac8I GCNNGC 1 cut(s) 202
CciI TCATGA 1 cut(s) 1132
CfoI GCGC 1 cut(s) 273
Cfr13I GGNCC 1 cut(s) 491
Cfr42I CCGCGG 1 cut(s) 64
CseI GACGC 2 cut(s) 96, 171
Csp6I GTAC 5 cut(s) 220, 258, 381, 682, 966
CspCI CAANNNNNGTGG 2 cut(s) 610, 645
CviAII CATG 2 cut(s) 823, 1133
CviQI GTAC 5 cut(s) 220, 258, 381, 682, 966
DdeI CTNAG 3 cut(s) 678, 1145, 1320
DpnI GATC 9 cut(s) 123, 207, 468, 537, 676, 711, 793, 802, 1251
DpnII GATC 9 cut(s) 121, 205, 466, 535, 674, 709, 791, 800, 1249
Eam1104I CTCTTC 3 cut(s) 438, 537, 588
EarI CTCTTC 3 cut(s) 438, 537, 588
Ecl136II GAGCTC 2 cut(s) 312, 740
Eco130I CCWWGG 1 cut(s) 1254
Eco24I GRGCYC 2 cut(s) 314, 742
Eco47I GGWCC 1 cut(s) 491
Eco53kI GAGCTC 2 cut(s) 312, 740
Eco57I CTGAAG 1 cut(s) 152
EcoICRI GAGCTC 2 cut(s) 312, 740
EcoO109I RGGNCCY 1 cut(s) 491
EcoRII CCWGG 3 cut(s) 124, 383, 493
EcoT14I CCWWGG 1 cut(s) 1254
EcoT38I GRGCYC 2 cut(s) 314, 742
ErhI CCWWGG 1 cut(s) 1254
Esp3I CGTCTC 1 cut(s) 1284
FaeI CATG 2 cut(s) 826, 1136
FalI AAGNNNNNCTT 2 cut(s) 1333, 1365
FaqI GGGAC 1 cut(s) 92
FatI CATG 2 cut(s) 822, 1132
FauI CCCGC 1 cut(s) 143
FauNDI CATATG 1 cut(s) 102
FblI GTMKAC 1 cut(s) 1260
Fnu4HI GCNGC 6 cut(s) 157, 297, 450, 453, 474, 1005
FokI GGATG 3 cut(s) 267, 974, 1073
FriOI GRGCYC 2 cut(s) 314, 742
Fsp4HI GCNGC 6 cut(s) 157, 297, 450, 453, 474, 1005
FspBI CTAG 3 cut(s) 456, 639, 819
GlaI GCGC 1 cut(s) 272
GluI GCNGC 6 cut(s) 157, 297, 450, 453, 474, 1005
HgaI GACGC 2 cut(s) 96, 171
HhaI GCGC 1 cut(s) 273
Hin1I GRCGYC 3 cut(s) 107, 182, 1277
Hin1II CATG 2 cut(s) 826, 1136
Hin6I GCGC 1 cut(s) 271
HinP1I GCGC 1 cut(s) 271
HinfI GANTC 6 cut(s) 22, 80, 327, 388, 897, 972
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 3 cut(s) 220, 421, 1261
Hpy188I TCNGA 6 cut(s) 121, 132, 187, 679, 835, 1230
Hpy188III TCNNGA 5 cut(s) 539, 609, 804, 1133, 1302
Hpy8I GTNNAC 3 cut(s) 220, 421, 1261
Hpy99I CGWCG 4 cut(s) 112, 115, 187, 658
HpyAV CCTTC 4 cut(s) 12, 400, 1219, 1308
HpyCH4III ACNGT 3 cut(s) 323, 851, 999
HpyCH4IV ACGT 1 cut(s) 1277
HpyCH4V TGCA 6 cut(s) 452, 550, 747, 1021, 1055, 1220
HpyF10VI GCNNNNNNNGC 5 cut(s) 156, 162, 188, 197, 458
HpyF3I CTNAG 3 cut(s) 678, 1145, 1320
HpySE526I ACGT 1 cut(s) 1277
Hsp92I GRCGYC 3 cut(s) 107, 182, 1277
Hsp92II CATG 2 cut(s) 826, 1136
HspAI GCGC 1 cut(s) 271
KpnI GGTACC 1 cut(s) 969
KspI CCGCGG 1 cut(s) 64
Kzo9I GATC 9 cut(s) 121, 205, 466, 535, 674, 709, 791, 800, 1249
LmnI GCTCC 7 cut(s) 173, 309, 317, 649, 737, 1210, 1291
Lsp1109I GCAGC 5 cut(s) 283, 436, 460, 464, 991
LweI GCATC 6 cut(s) 282, 537, 912, 945, 996, 1040
MaeI CTAG 3 cut(s) 456, 639, 819
MaeII ACGT 1 cut(s) 1277
MaeIII GTNAC 3 cut(s) 328, 517, 773
MalI GATC 9 cut(s) 123, 207, 468, 537, 676, 711, 793, 802, 1251
MboI GATC 9 cut(s) 121, 205, 466, 535, 674, 709, 791, 800, 1249
MboII GAAGA 9 cut(s) 145, 244, 253, 455, 554, 575, 600, 848, 1286
MfeI CAATTG 2 cut(s) 84, 1113
MflI RGATCY 4 cut(s) 121, 535, 674, 800
MhlI GDGCHC 2 cut(s) 314, 742
MluCI AATT 7 cut(s) 84, 508, 615, 623, 827, 1113, 1305
MlyI GAGTC 4 cut(s) 16, 74, 336, 981
MmeI TCCRAC 3 cut(s) 165, 819, 838
MseI TTAA 2 cut(s) 96, 879
MslI CAYNNNNRTG 3 cut(s) 702, 852, 889
MspA1I CMGCKG 2 cut(s) 63, 1004
MspR9I CCNGG 3 cut(s) 126, 385, 495
MunI CAATTG 2 cut(s) 84, 1113
MvaI CCWGG 3 cut(s) 126, 385, 495
MvnI CGCG 1 cut(s) 63
MwoI GCNNNNNNNGC 5 cut(s) 156, 162, 188, 197, 458
NdeI CATATG 1 cut(s) 102
NdeII GATC 9 cut(s) 121, 205, 466, 535, 674, 709, 791, 800, 1249
NlaIII CATG 2 cut(s) 826, 1136
NlaIV GGNNCC 5 cut(s) 123, 492, 651, 967, 1206
NmeAIII GCCGAG 1 cut(s) 313
NmuCI GTSAC 1 cut(s) 328
NspV TTCGAA 1 cut(s) 586
OliI CACNNNNGTG 1 cut(s) 852
PagI TCATGA 1 cut(s) 1132
PfeI GAWTC 2 cut(s) 388, 897
PflMI CCANNNNNTGG 2 cut(s) 1096, 1272
PkrI GCNGC 6 cut(s) 158, 298, 451, 454, 475, 1006
PleI GAGTC 4 cut(s) 16, 74, 335, 980
PpsI GAGTC 4 cut(s) 16, 74, 335, 980
PpuMI RGGWCCY 1 cut(s) 491
Psp124BI GAGCTC 2 cut(s) 314, 742
Psp5II RGGWCCY 1 cut(s) 491
Psp6I CCWGG 3 cut(s) 124, 383, 493
PspGI CCWGG 3 cut(s) 124, 383, 493
PspN4I GGNNCC 5 cut(s) 123, 492, 651, 967, 1206
PspPI GGNCC 1 cut(s) 491
PspPPI RGGWCCY 1 cut(s) 491
PstI CTGCAG 1 cut(s) 454
PsuI RGATCY 4 cut(s) 121, 535, 674, 800
PvuII CAGCTG 1 cut(s) 1004
RsaI GTAC 5 cut(s) 221, 259, 382, 683, 967
RsaNI GTAC 5 cut(s) 220, 258, 381, 682, 966
RseI CAYNNNNRTG 3 cut(s) 702, 852, 889
SacI GAGCTC 2 cut(s) 314, 742
SacII CCGCGG 1 cut(s) 64
SaqAI TTAA 2 cut(s) 96, 879
SatI GCNGC 6 cut(s) 157, 297, 450, 453, 474, 1005
Sau3AI GATC 9 cut(s) 121, 205, 466, 535, 674, 709, 791, 800, 1249
Sau96I GGNCC 1 cut(s) 491
ScaI AGTACT 1 cut(s) 683
SchI GAGTC 4 cut(s) 16, 74, 336, 981
ScrFI CCNGG 3 cut(s) 126, 385, 495
SduI GDGCHC 2 cut(s) 314, 742
SfaNI GCATC 6 cut(s) 282, 537, 912, 945, 996, 1040
SfcI CTRYAG 1 cut(s) 450
Sfr303I CCGCGG 1 cut(s) 64
SfuI TTCGAA 1 cut(s) 586
SgrBI CCGCGG 1 cut(s) 64
SinI GGWCC 1 cut(s) 491
SmiMI CAYNNNNRTG 3 cut(s) 702, 852, 889
SmlI CTYRAG 1 cut(s) 607
SmoI CTYRAG 1 cut(s) 607
Sse9I AATT 7 cut(s) 84, 508, 615, 623, 827, 1113, 1305
SsiI CCGC 7 cut(s) 61, 63, 144, 150, 156, 165, 191
SspMI CTAG 3 cut(s) 456, 639, 819
SstI GAGCTC 2 cut(s) 314, 742
StyD4I CCNGG 3 cut(s) 124, 383, 493
StyI CCWWGG 1 cut(s) 1254
TaaI ACNGT 3 cut(s) 323, 851, 999
TaiI ACGT 1 cut(s) 1280
TaqI TCGA 3 cut(s) 239, 586, 805
TaqII GACCGA 1 cut(s) 152
TasI AATT 7 cut(s) 84, 508, 615, 623, 827, 1113, 1305
TatI WGTACW 2 cut(s) 219, 681
TauI GCSGC 1 cut(s) 159
TfiI GAWTC 2 cut(s) 388, 897
Tru1I TTAA 2 cut(s) 96, 879
Tru9I TTAA 2 cut(s) 96, 879
TscAI CASTG 2 cut(s) 166, 604
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 5 cut(s) 296, 449, 452, 473, 1004
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 4 cut(s) 17, 456, 839, 1086
TspGWI ACGGA 2 cut(s) 279, 322
TspRI CASTG 2 cut(s) 166, 604
Van91I CCANNNNNTGG 2 cut(s) 1096, 1272
VpaK11BI GGWCC 1 cut(s) 491
XapI RAATTY 4 cut(s) 615, 623, 827, 1305
XmiI GTMKAC 1 cut(s) 1260
XspI CTAG 3 cut(s) 456, 639, 819
ZraI GACGTC 1 cut(s) 1278
ZrmI AGTACT 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.