Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0007765

Physical Location & Seq

Genomic Coordinates Reverse (-)

17953608 .. 17959435

Gene Structure 5828 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3483 bp

ATGTACAATCCCCTTTCCTCTTCCTTTTTCCCTCTTTTTTCTCAGTCGAGAGTCACAGCCTCCCAGTCCTCTCTCTCCTCTCTCTATCTCGCACTTTCTCTCTCTACTGTATCAGTCTCTCTCTCTGTGAAAATGGGGGTGGCAGAGCGACCCACAGTGCGAGTGACAAACATTCCGCAAACCGTCACAGCCCAAGAGCTATTAACGTTCCTCGAGTCGAAACTCGGCCGTGACTCGATCTTCGCCGTCGAAATCAGCAGCGATCACAAGAACTGGAAGTCACGTGGCTTCGGCCGCGTCCAGTTCACGACCCTCAAGGCCAAATTGGAAGCTCAGTCACTCTCCCTGAAAAACGACCTCGTTTTCAAGTCCGAAAGCCTCCGCCTTTCCGAGACCTACGACGACATCATCCAGCGGCCGGTGGACCAGAAGCGCCGGGTAAACGGCACCGTTTTGCATGTGGGTTTTATGGTGAAGGAAGATTGCATGAATGTGCTGGAGACGTGGGAGGACGTGAGGGCTTGGGTCATGCCCGAGAGGAAGCGGGTCGAGTTCTGGGTATGGCGGAAAGACGAGTGTTATAAGCTGGAGATTGCGTTTGAGAATGTTATGGATTCTGTTGGGTGCTGCTTGGGTGGCGAGAAAGTGAATGCCCTGCTTTTGAAGCTAAAGTTTGGTCCGAGGATCTTTCGAAAAGTTTCAGGACCTAACATAGCTGCCAAGTTCAGCGCTGATCGTTATCATGTTTGTAAGGAGGATTATGATTACCTCTGGGTACGAACTACTGATTTTTCGGACATGAAGTCCCTCGGTTATGCTACTTCATTCTGCTGGGAGATTGAGGAGGAGTTTTCTGTGCCGGATGTATTCGAGTGTTTCCCGTTCTATAAAGATAATGATGTGGTGGATCTCATTTTAGAGGACGGTGAAAGATACTCTTTGCGGTCGGAGACAGTTCCACTTGTAAACTGCGGATCAAATTCAAAGTTGCCATATGAGGTCCTTTTCCAAATCAATGCACTCGTTCATTCCCAGAAGATTAGTCTTGCAGCGGCGGACTCTGATCTGATTGAGTTCCTTAGTGGTTTAAGTGTAGACACTGCTAATGCGCTTCTTGAGAAGCTGCACAAGCGCAATACCATTTGCTACGACCCTCTATTATTCTTAAGGATTCAATCACATGACTTGGAAGGAAAGTCTAAGAGTCGTCCCTCAGCCCATAAAAGATTAGTGGAACATAACGTAATGAGTGTTCATAGAGCCTTAATTACCCCATCAAAGATTTGTTGCTGTGGGCCTGAGCTTGAAAAATCAAACTATGTTGTGAAGAACTTCGCAGACTATGCTTCCGATTTTATGAGAGTTACATTCGTTGATGAGGATTGGGGTAAGCTTTCTCCAAATGCTCTTTCCACAAGCATCCACAAAGGTATGTTTACAAAACCCCATAGAACTGGAATATATCATCGGATATTGTCTATTCTTCGAGATGGGATTGTGATAGGGGAAAAAAAATTTGAGTTTCTGGCTTTCTCGGCTAGTCAACTTCGATCCAACTCTGTTTGGATGTTTTCTTCTAATGACAAAGTAAAAGCGGAAGATATTAGAGAATGGATGGGCTGCTTCACCAAAATCCGGAGCGTATCTAAGTGTGCAGCTAGGATGGGCCAACTATTCAGTTCTTCTACACAAACTCTTGTTGTTCCTGCACAAGATGTAGAGGTTATTCCTGACGTTGAGACTTCTTCTGATGGAGTTACTTACTGCTTCTCGGATGGTATAGGAAAGATTTCTCTATCTTTTGCCAGGCAAATTGCTCACAAGTGTGGTTTGGATCATATTCCTTCAGCATTTCAAATTCGATATGGTGGATATAAAGGGGTTATAGCTGTTGACCGTAATTCCTTTCGGAAGCTATCTTTGCGTGGCAGTATGCTCAAATTTGAGTCAAAAAACAGAATGCTCAATGTTACCAAATGGACTGATTCCTCGCCCTGCTATCTGAATCGAGAGATTATTATCCTCATGTCTACCTTAGGGGTAAAGGATGAAACATTTGAGGCGTTGCAAGAGGAACAATTGCGTCTTCTGGGGAAAATGCGGACAGAAAGAGCTGCAGCGTTGAGTGTCTTAGAGGGATTCAGTGGGGCTAGTTCCAGAAATATTCTTGTAAAGATGTTACATCATGGTTATGAACCAAATGTGGAACCTTACCTCTCAATGATGCTTCAGTCATATTATGAGAACTACTTATCTGATATAAAAAGTAGATGCCGCATATACGTCCCGAAAGGCCGGATCCTGGTTGGGTGCTTGGATGAAACTGGAAGTTTAAACTATGGTCAAGTTTATGTTCGAATAACCATGACAAAAGATGAGCTAGAAATGGGGGATCAGAGTTTCTTCCAGAAGGTTGATGAAACAACATGTGTTGTAACCGGGAAGGTGGTTGTCACAAAAAATCCTTGTCTTCACCCAGGAGATGTCCGGGTACTAGAGGCTGTCTATGATGTGGTACTGGAGGAGAAGAATTTGATGGATTGCCTTATCTTTCCCCAAAAAGGAGAAAGGCCTCATCCAAATGAATGTTCCGGTGGTGATCTTGATGGAGACTTGTTTTTCATAAGTTGGGATAAAGATCTGGTCCCTCCTCGTACTGTACCTCCCATGGACTACTCTGCACGGAGACCTCGGACGATGGATCACGATGTAACCTTAGAGGAAATTCAAAAATTCTTTGTTGATTACATGATAAATGACAATTTGGGTGCCATCTCTACTGCACATCTAGTTCATGCCGACCGTGAACCAGACAAAGCTTTGGATTCAAAATGCCTGCAGTTGGCAGACCTGCATTCCCAGGCTGTTGACTTTGCAAAAACTGGTGCACCGGCTGAAATGCCCCGGATCTTAAAACCAAAAGAGTTTCCAGATTTCATGGAAAGGTGGGAAAAGCCCATGTACTTCTCGAACGGGGCATTAGGGAAGCTGTACCGTGCATGTGTTGGCTCAGTGGTGCAGGAAAAAAGAGGCTTGGTTTGGTCAGAGCCGATTGCTGAAGCAGCTTACGATCAGGATCTTCAAGTAGGTGGTATCGAGTCAGTTCTTGAAGTCGCAAAAGGTCACAGAGACCTGTACATAGAGAAGATGAAAAGCATAATGAACTACTATGGGGCCAGGACCGAAGATGAAATATTGACAGGCAATCTTCGATATCGTGCAGCATATCTGCAACGTGATAACAGGAGATACGGGGATATGAAGGATCGGATATCGCTGACATTCAAGAACCTACAGAAAGAAGCGAAGGGGTGGTTCGAGAGTAGCTGCAGAGACGGTGAGCATGAGAAACTGGCGTCGGCTTGGTACCATGTAACTTATCATCCACTGCATTTCCGAGAAGACATGCACTGCCTGAGCTTTCCATGGGTTGTGGGTGATATTTTACTGAGCATAAAGTCGGCTAAAATCCAAACAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0006139 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010495 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031048 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0034641 GO:0035194 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0070919 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0098542 GO:1901360 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RdRP

Protein Analysis

1161

Amino Acids

131.96

Weight (kDa)

6.58

Isoelectric Point (pI)

40.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RRM_RdRP1_2	PF26250	52 - 135	9.1e-27	RdRP1/2, RRM domain
PH_RDR2	PF24823	152 - 299	2.4e-66	RDR2 PH-like domain
RdRP_helical	PF26252	320 - 401	1.6e-14	RNA dependent RNA polymerase, helical domain
RdRP	PF05183	423 - 1000	2.3e-191	RNA dependent RNA polymerase
RdRP_head	PF26253	1022 - 1155	6.2e-39	RdRP, head domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007765)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G11130
fragaria_vesca	FvH4_5g05070 FvH4_5g05070 FvH4_5g05070
malus_domestica	MD14G1184100.v1.1
prunus_persica	Prupe.5G176700_v2.0.a1
pyrus_communis	pycom14g15310
rosa_chinensis	RchiOBHm_Chr7g0193931
rosa_laevigata	RLG00000004228 RLG00000004230 RLG00000004232 RLG00000034353
rosa_multiflora	Rmu_co8198578.1_g000001 Rmu_co8264681.1_g000001 Rmu_co8480069.1_g000001 Rmu_sc0002389.1_g000009 Rmu_sc0031476.1_g000001
rosa_roxburghii	Rroxscaffold_3G00261680
rosa_rugosa	Rorug07G0012900
rosa_samantha	Rh7AG139700 Rh7BG140300 Rh7CG143900 Rh7DG141100
rosa_wichuraiana	Rw7G011920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	582
Acc36I	ACCTGC	1 cut(s)	2859
Acc65I	GGTACC	1 cut(s)	3366
AccB1I	GGYRCC	3 cut(s)	446, 2768, 3366
AccI	GTMKAC	2 cut(s)	1095, 2030
AccII	CGCG	1 cut(s)	297
AccIII	TCCGGA	1 cut(s)	1635
AclI	AACGTT	1 cut(s)	206
AcoI	YGGCCR	3 cut(s)	226, 292, 416
AcsI	RAATTY	7 cut(s)	979, 1514, 1857, 1940, 2530, 2724, 2732
AcuI	CTGAAG	3 cut(s)	1830, 2213, 3078
AcvI	CACGTG	1 cut(s)	284
AcyI	GRCGYC	1 cut(s)	3356
AfeI	AGCGCT	1 cut(s)	730
AfiI	CCNNNNNNNGG	5 cut(s)	418, 1635, 2302, 2561, 2842
AflII	CTTAAG	1 cut(s)	1165
AflIII	ACRYGT	1 cut(s)	2426
AhdI	GACNNNNNGTC	1 cut(s)	802
AjiI	CACGTC	2 cut(s)	504, 514
AjnI	CCWGG	5 cut(s)	1805, 2301, 2476, 2859, 3176
AjuI	GAANNNNNNNTTGG	4 cut(s)	1393, 1425, 2572, 2604
AleI	CACNNNNGTG	1 cut(s)	1824
Alw21I	GWGCWC	1 cut(s)	2890
Alw26I	GTCTC	9 cut(s)	121, 386, 494, 944, 1733, 2604, 2680, 3123, 3327
Alw44I	GTGCAC	1 cut(s)	2886
Ama87I	CYCGRG	2 cut(s)	212, 533
Aor13HI	TCCGGA	1 cut(s)	1635
Aor51HI	AGCGCT	1 cut(s)	730
AoxI	GGCC	9 cut(s)	226, 292, 318, 416, 1295, 1666, 2293, 2570, 3174
ApaLI	GTGCAC	1 cut(s)	2886
ApoI	RAATTY	7 cut(s)	979, 1514, 1857, 1940, 2530, 2724, 2732
ArsI	GACNNNNNNTTYG	2 cut(s)	224, 256
Asp700I	GAANNNNTTC	3 cut(s)	697, 1331, 1789
Asp718I	GGTACC	1 cut(s)	3366
AspLEI	GCGC	4 cut(s)	435, 731, 1111, 1134
AspS9I	GGNCC	9 cut(s)	424, 677, 704, 1000, 1295, 1666, 2644, 3174, 3180
AsuC2I	CCSGG	4 cut(s)	437, 2440, 2489, 2905
AsuHPI	GGTGA	7 cut(s)	484, 938, 1618, 2465, 2609, 3350, 3449
AsuII	TTCGAA	2 cut(s)	691, 2356
AvaI	CYCGRG	2 cut(s)	212, 533
AvaII	GGWCC	6 cut(s)	424, 677, 704, 1000, 2644, 3180
AxyI	CCTNAGG	1 cut(s)	2035
BaeGI	GKGCMC	1 cut(s)	2890
BamHI	GGATCC	1 cut(s)	2298
BanI	GGYRCC	3 cut(s)	446, 2768, 3366
BbrPI	CACGTG	1 cut(s)	284
BbsI	GAAGAC	3 cut(s)	2078, 2462, 3408
Bbv12I	GWGCWC	1 cut(s)	2890
BbvCI	CCTCAGC	1 cut(s)	1213
BceAI	ACGGC	3 cut(s)	213, 230, 460
BciT130I	CCWGG	5 cut(s)	1807, 2303, 2478, 2861, 3178
BcnI	CCSGG	4 cut(s)	437, 2440, 2489, 2905
BcoDI	GTCTC	9 cut(s)	121, 386, 494, 944, 1733, 2604, 2680, 3123, 3327
BfaI	CTAG	6 cut(s)	1539, 1659, 2151, 2381, 2495, 2789
BfmI	CTRYAG	4 cut(s)	2115, 2837, 3293, 3328
BfoI	RGCGCY	2 cut(s)	436, 732
BfrI	CTTAAG	1 cut(s)	1165
BfuAI	ACCTGC	1 cut(s)	2859
BglII	AGATCT	1 cut(s)	2638
Bme1390I	CCNGG	9 cut(s)	437, 1807, 2303, 2440, 2478, 2489, 2861, 2905, 3178
Bme18I	GGWCC	6 cut(s)	424, 677, 704, 1000, 2644, 3180
BmeRI	GACNNNNNGTC	1 cut(s)	802
BmeT110I	CYCGRG	2 cut(s)	212, 533
BmgBI	CACGTC	2 cut(s)	504, 514
BmgT120I	GGNCC	9 cut(s)	424, 677, 704, 1000, 1295, 1666, 2644, 3174, 3180
BmiI	GGNNCC	7 cut(s)	448, 2208, 2300, 2646, 2770, 3175, 3368
BmrFI	CCNGG	9 cut(s)	437, 1807, 2303, 2440, 2478, 2489, 2861, 2905, 3178
BmrI	ACTGGG	1 cut(s)	58
BmsI	GCATC	3 cut(s)	1428, 2214, 2261
BmuI	ACTGGG	1 cut(s)	58
BpiI	GAAGAC	3 cut(s)	2078, 2462, 3408
BpmI	CTGGAG	3 cut(s)	518, 608, 2540
Bpu10I	CCTNAGC	3 cut(s)	1213, 1299, 3416
Bpu14I	TTCGAA	2 cut(s)	691, 2356
BpuEI	CTTGAG	2 cut(s)	299, 1136
BpuMI	CCSGG	4 cut(s)	437, 2440, 2489, 2905
BsaAI	YACGTR	1 cut(s)	284
BsaBI	GATNNNNATC	4 cut(s)	738, 2018, 2637, 3075
BsaHI	GRCGYC	1 cut(s)	3356
BsaI	GGTCTC	3 cut(s)	386, 2680, 3123
BsaJI	CCNNGG	8 cut(s)	680, 808, 2476, 2667, 2690, 2859, 2903, 3425
BsaWI	WCCGGW	2 cut(s)	1635, 2591
BsaXI	ACNNNNNCTCC	4 cut(s)	581, 611, 2647, 2677
Bsc4I	CCNNNNNNNGG	5 cut(s)	418, 1635, 2302, 2561, 2842
Bse118I	RCCGGY	2 cut(s)	418, 2890
Bse1I	ACTGG	8 cut(s)	64, 278, 301, 1459, 2329, 2523, 2887, 3357
Bse21I	CCTNAGG	1 cut(s)	2035
Bse8I	GATNNNNATC	4 cut(s)	738, 2018, 2637, 3075
BseAI	TCCGGA	1 cut(s)	1635
BseBI	CCWGG	5 cut(s)	1807, 2303, 2478, 2861, 3178
BseDI	CCNNGG	8 cut(s)	680, 808, 2476, 2667, 2690, 2859, 2903, 3425
BseJI	GATNNNNATC	4 cut(s)	738, 2018, 2637, 3075
BseLI	CCNNNNNNNGG	5 cut(s)	418, 1635, 2302, 2561, 2842
BseMII	CTCAG	7 cut(s)	56, 347, 1227, 1290, 3024, 3407, 3440
BseNI	ACTGG	8 cut(s)	64, 278, 301, 1459, 2329, 2523, 2887, 3357
BseRI	GAGGAG	5 cut(s)	67, 857, 860, 2537, 2640
BseSI	GKGCMC	1 cut(s)	2890
BseX3I	CGGCCG	3 cut(s)	226, 292, 416
BseYI	CCCAGC	1 cut(s)	831
BsgI	GTGCAG	7 cut(s)	1109, 1674, 1692, 2664, 2766, 3038, 3240
Bsh1236I	CGCG	1 cut(s)	297
Bsh1285I	CGRYCG	5 cut(s)	229, 295, 419, 947, 2803
BshFI	GGCC	9 cut(s)	228, 294, 320, 418, 1297, 1668, 2295, 2572, 3176
BshNI	GGYRCC	3 cut(s)	446, 2768, 3366
BsiEI	CGRYCG	5 cut(s)	229, 295, 419, 947, 2803
BsiHKAI	GWGCWC	1 cut(s)	2890
BsiHKCI	CYCGRG	2 cut(s)	212, 533
BslFI	GGGAC	4 cut(s)	790, 1194, 2270, 2630
BslI	CCNNNNNNNGG	5 cut(s)	418, 1635, 2302, 2561, 2842
BsmAI	GTCTC	9 cut(s)	121, 386, 494, 944, 1733, 2604, 2680, 3123, 3327
BsmBI	CGTCTC	2 cut(s)	494, 3327
BsmFI	GGGAC	4 cut(s)	790, 1194, 2270, 2630
BsmI	GAATGC	3 cut(s)	655, 1965, 2854
BsnI	GGCC	9 cut(s)	228, 294, 320, 418, 1297, 1668, 2295, 2572, 3176
Bso31I	GGTCTC	3 cut(s)	386, 2680, 3123
BsoBI	CYCGRG	2 cut(s)	212, 533
Bsp119I	TTCGAA	2 cut(s)	691, 2356
Bsp1286I	GDGCHC	1 cut(s)	2890
Bsp13I	TCCGGA	1 cut(s)	1635
Bsp1407I	TGTACA	2 cut(s)	3, 3135
Bsp19I	CCATGG	2 cut(s)	2667, 3425
BspANI	GGCC	9 cut(s)	228, 294, 320, 418, 1297, 1668, 2295, 2572, 3176
BspCNI	CTCAG	7 cut(s)	55, 346, 1226, 1291, 3023, 3408, 3441
BspEI	TCCGGA	1 cut(s)	1635
BspFNI	CGCG	1 cut(s)	297
BspLI	GGNNCC	7 cut(s)	448, 2208, 2300, 2646, 2770, 3175, 3368
BspMAI	CTGCAG	3 cut(s)	2119, 2841, 3332
BspMI	ACCTGC	1 cut(s)	2859
BspT104I	TTCGAA	2 cut(s)	691, 2356
BspT107I	GGYRCC	3 cut(s)	446, 2768, 3366
BspTI	CTTAAG	1 cut(s)	1165
BspTNI	GGTCTC	3 cut(s)	386, 2680, 3123
BsrFI	RCCGGY	2 cut(s)	418, 2890
BsrGI	TGTACA	2 cut(s)	3, 3135
BsrI	ACTGG	8 cut(s)	64, 278, 301, 1459, 2329, 2523, 2887, 3357
BssAI	RCCGGY	2 cut(s)	418, 2890
BssECI	CCNNGG	8 cut(s)	680, 808, 2476, 2667, 2690, 2859, 2903, 3425
BssNI	GRCGYC	1 cut(s)	3356
BssT1I	CCWWGG	2 cut(s)	2667, 3425
Bst2UI	CCWGG	5 cut(s)	1807, 2303, 2478, 2861, 3178
Bst6I	CTCTTC	1 cut(s)	25
BstACI	GRCGYC	1 cut(s)	3356
BstAFI	CTTAAG	1 cut(s)	1165
BstAPI	GCANNNNNTGC	1 cut(s)	1343
BstAUI	TGTACA	2 cut(s)	3, 3135
BstBAI	YACGTR	1 cut(s)	284
BstBI	TTCGAA	2 cut(s)	691, 2356
BstC8I	GCNNGC	1 cut(s)	2837
BstDSI	CCRYGG	2 cut(s)	2667, 3425
BstFNI	CGCG	1 cut(s)	297
BstH2I	RGCGCY	2 cut(s)	436, 732
BstHHI	GCGC	4 cut(s)	435, 731, 1111, 1134
BstMAI	GTCTC	9 cut(s)	121, 386, 494, 944, 1733, 2604, 2680, 3123, 3327
BstMCI	CGRYCG	5 cut(s)	229, 295, 419, 947, 2803
BstMWI	GCNNNNNNNGC	8 cut(s)	294, 636, 664, 1129, 1343, 1535, 1921, 3062
BstNI	CCWGG	5 cut(s)	1807, 2303, 2478, 2861, 3178
BstNSI	RCATGY	4 cut(s)	461, 2430, 3003, 3409
BstSCI	CCNGG	9 cut(s)	435, 1805, 2301, 2438, 2476, 2487, 2859, 2903, 3176
BstSFI	CTRYAG	4 cut(s)	2115, 2837, 3293, 3328
BstSLI	GKGCMC	1 cut(s)	2890
BstUI	CGCG	1 cut(s)	297
BstV2I	GAAGAC	3 cut(s)	2078, 2462, 3408
BstX2I	RGATCY	6 cut(s)	684, 907, 2298, 2638, 2907, 3076
BstXI	CCANNNNNNTGG	1 cut(s)	1454
BstYI	RGATCY	6 cut(s)	684, 907, 2298, 2638, 2907, 3076
BstZI	CGGCCG	3 cut(s)	226, 292, 416
Bsu36I	CCTNAGG	1 cut(s)	2035
BsuRI	GGCC	9 cut(s)	228, 294, 320, 418, 1297, 1668, 2295, 2572, 3176
BtgI	CCRYGG	2 cut(s)	2667, 3425
BtrI	CACGTC	2 cut(s)	504, 514
BtsI	GCAGTG	3 cut(s)	1098, 3386, 3409
BtsIMutI	CAGTG	6 cut(s)	162, 1098, 2149, 3018, 3386, 3409
BveI	ACCTGC	1 cut(s)	2859
Cac8I	GCNNGC	1 cut(s)	2837
CfoI	GCGC	4 cut(s)	435, 731, 1111, 1134
Cfr10I	RCCGGY	2 cut(s)	418, 2890
Cfr13I	GGNCC	9 cut(s)	424, 677, 704, 1000, 1295, 1666, 2644, 3174, 3180
CseI	GACGC	3 cut(s)	286, 2072, 3345
DraI	TTTAAA	1 cut(s)	2334
DriI	GACNNNNNGTC	1 cut(s)	802
EaeI	YGGCCR	3 cut(s)	226, 292, 416
EagI	CGGCCG	3 cut(s)	226, 292, 416
Eam1104I	CTCTTC	1 cut(s)	25
Eam1105I	GACNNNNNGTC	1 cut(s)	802
EarI	CTCTTC	1 cut(s)	25
EciI	GGCGGA	3 cut(s)	371, 580, 1070
EclXI	CGGCCG	3 cut(s)	226, 292, 416
Eco130I	CCWWGG	2 cut(s)	2667, 3425
Eco147I	AGGCCT	1 cut(s)	2572
Eco31I	GGTCTC	3 cut(s)	386, 2680, 3123
Eco32I	GATATC	2 cut(s)	3215, 3273
Eco47I	GGWCC	6 cut(s)	424, 677, 704, 1000, 2644, 3180
Eco47III	AGCGCT	1 cut(s)	730
Eco52I	CGGCCG	3 cut(s)	226, 292, 416
Eco57I	CTGAAG	3 cut(s)	1830, 2213, 3078
Eco72I	CACGTG	1 cut(s)	284
Eco81I	CCTNAGG	1 cut(s)	2035
Eco88I	CYCGRG	2 cut(s)	212, 533
EcoO109I	RGGNCCY	2 cut(s)	704, 1000
EcoRII	CCWGG	5 cut(s)	1805, 2301, 2476, 2859, 3176
EcoRV	GATATC	2 cut(s)	3215, 3273
EcoT14I	CCWWGG	2 cut(s)	2667, 3425
ErhI	CCWWGG	2 cut(s)	2667, 3425
Esp3I	CGTCTC	2 cut(s)	494, 3327
FalI	AAGNNNNNCTT	2 cut(s)	922, 954
FaqI	GGGAC	4 cut(s)	790, 1194, 2270, 2630
FauI	CCCGC	1 cut(s)	537
FauNDI	CATATG	1 cut(s)	994
FblI	GTMKAC	2 cut(s)	1095, 2030
FspBI	CTAG	6 cut(s)	1539, 1659, 2151, 2381, 2495, 2789
GlaI	GCGC	4 cut(s)	434, 730, 1110, 1133
GsaI	CCCAGC	1 cut(s)	835
GsuI	CTGGAG	3 cut(s)	518, 608, 2540
HaeII	RGCGCY	2 cut(s)	436, 732
HaeIII	GGCC	9 cut(s)	228, 294, 320, 418, 1297, 1668, 2295, 2572, 3176
HgaI	GACGC	3 cut(s)	286, 2072, 3345
HhaI	GCGC	4 cut(s)	435, 731, 1111, 1134
Hin1I	GRCGYC	1 cut(s)	3356
Hin6I	GCGC	4 cut(s)	433, 729, 1109, 1132
HinP1I	GCGC	4 cut(s)	433, 729, 1109, 1132
HincII	GTYRAC	3 cut(s)	1544, 1894, 2869
HindII	GTYRAC	3 cut(s)	1544, 1894, 2869
HindIII	AAGCTT	2 cut(s)	1391, 2817
HphI	GGTGA	7 cut(s)	484, 938, 1618, 2465, 2609, 3350, 3449
Hpy99I	CGWCG	3 cut(s)	251, 404, 3361
HpyAV	CCTTC	7 cut(s)	469, 1184, 1854, 2404, 2437, 3256, 3301
HpyCH4IV	ACGT	8 cut(s)	206, 283, 503, 513, 1242, 1734, 2283, 3235
HpyF10VI	GCNNNNNNNGC	8 cut(s)	294, 636, 664, 1129, 1343, 1535, 1921, 3062
HpySE526I	ACGT	8 cut(s)	206, 283, 503, 513, 1242, 1734, 2283, 3235
Hsp92I	GRCGYC	1 cut(s)	3356
HspAI	GCGC	4 cut(s)	433, 729, 1109, 1132
Kpn2I	TCCGGA	1 cut(s)	1635
KpnI	GGTACC	1 cut(s)	3370
LmnI	GCTCC	1 cut(s)	1638
LweI	GCATC	3 cut(s)	1428, 2214, 2261
MaeI	CTAG	6 cut(s)	1539, 1659, 2151, 2381, 2495, 2789
MaeII	ACGT	8 cut(s)	206, 283, 503, 513, 1242, 1734, 2283, 3235
MfeI	CAATTG	1 cut(s)	2078
MflI	RGATCY	6 cut(s)	684, 907, 2298, 2638, 2907, 3076
MhlI	GDGCHC	1 cut(s)	2890
MlyI	GAGTC	7 cut(s)	60, 224, 227, 1052, 1213, 1955, 3107
MmeI	TCCRAC	2 cut(s)	927, 1578
MroI	TCCGGA	1 cut(s)	1635
MroXI	GAANNNNTTC	3 cut(s)	697, 1331, 1789
MseI	TTAA	6 cut(s)	203, 1088, 1166, 1265, 2333, 2912
MslI	CAYNNNNRTG	4 cut(s)	491, 1824, 2190, 2580
MspA1I	CMGCKG	2 cut(s)	415, 1052
MspCI	CTTAAG	1 cut(s)	1165
MspR9I	CCNGG	9 cut(s)	437, 1807, 2303, 2440, 2478, 2489, 2861, 2905, 3178
MssI	GTTTAAAC	1 cut(s)	2334
MunI	CAATTG	1 cut(s)	2078
Mva1269I	GAATGC	3 cut(s)	655, 1965, 2854
MvaI	CCWGG	5 cut(s)	1807, 2303, 2478, 2861, 3178
MvnI	CGCG	1 cut(s)	297
MwoI	GCNNNNNNNGC	8 cut(s)	294, 636, 664, 1129, 1343, 1535, 1921, 3062
NciI	CCSGG	4 cut(s)	437, 2440, 2489, 2905
NcoI	CCATGG	2 cut(s)	2667, 3425
NdeI	CATATG	1 cut(s)	994
NlaIV	GGNNCC	7 cut(s)	448, 2208, 2300, 2646, 2770, 3175, 3368
NmeAIII	GCCGAG	2 cut(s)	204, 1514
NmuCI	GTSAC	8 cut(s)	52, 163, 184, 230, 279, 336, 2452, 3122
NspI	RCATGY	4 cut(s)	461, 2430, 3003, 3409
NspV	TTCGAA	2 cut(s)	691, 2356
OliI	CACNNNNGTG	1 cut(s)	1824
PaeR7I	CTCGAG	1 cut(s)	212
PceI	AGGCCT	1 cut(s)	2572
PciI	ACATGT	1 cut(s)	2426
PctI	GAATGC	3 cut(s)	655, 1965, 2854
PdmI	GAANNNNTTC	3 cut(s)	697, 1331, 1789
PfeI	GAWTC	6 cut(s)	614, 1171, 1985, 2005, 2139, 2825
PleI	GAGTC	7 cut(s)	59, 223, 227, 1052, 1212, 1954, 3106
PmaCI	CACGTG	1 cut(s)	284
PmeI	GTTTAAAC	1 cut(s)	2334
PmlI	CACGTG	1 cut(s)	284
PpsI	GAGTC	7 cut(s)	59, 223, 227, 1052, 1212, 1954, 3106
Ppu21I	YACGTR	1 cut(s)	284
PpuMI	RGGWCCY	2 cut(s)	704, 1000
PscI	ACATGT	1 cut(s)	2426
PsiI	TTATAA	1 cut(s)	582
Psp1406I	AACGTT	1 cut(s)	206
Psp5II	RGGWCCY	2 cut(s)	704, 1000
Psp6I	CCWGG	5 cut(s)	1805, 2301, 2476, 2859, 3176
PspCI	CACGTG	1 cut(s)	284
PspFI	CCCAGC	1 cut(s)	831
PspGI	CCWGG	5 cut(s)	1805, 2301, 2476, 2859, 3176
PspN4I	GGNNCC	7 cut(s)	448, 2208, 2300, 2646, 2770, 3175, 3368
PspPI	GGNCC	9 cut(s)	424, 677, 704, 1000, 1295, 1666, 2644, 3174, 3180
PspPPI	RGGWCCY	2 cut(s)	704, 1000
PspXI	VCTCGAGB	1 cut(s)	212
PsrI	GAACNNNNNNTAC	2 cut(s)	1236, 1268
PstI	CTGCAG	3 cut(s)	2119, 2841, 3332
PsuI	RGATCY	6 cut(s)	684, 907, 2298, 2638, 2907, 3076
RseI	CAYNNNNRTG	4 cut(s)	491, 1824, 2190, 2580
SaqAI	TTAA	6 cut(s)	203, 1088, 1166, 1265, 2333, 2912
Sau96I	GGNCC	9 cut(s)	424, 677, 704, 1000, 1295, 1666, 2644, 3174, 3180
SchI	GAGTC	7 cut(s)	60, 224, 227, 1052, 1213, 1955, 3107
ScrFI	CCNGG	9 cut(s)	437, 1807, 2303, 2440, 2478, 2489, 2861, 2905, 3178
SduI	GDGCHC	1 cut(s)	2890
SfaNI	GCATC	3 cut(s)	1428, 2214, 2261
SfcI	CTRYAG	4 cut(s)	2115, 2837, 3293, 3328
Sfr274I	CTCGAG	1 cut(s)	212
SfuI	TTCGAA	2 cut(s)	691, 2356
SinI	GGWCC	6 cut(s)	424, 677, 704, 1000, 2644, 3180
SlaI	CTCGAG	1 cut(s)	212
SmiMI	CAYNNNNRTG	4 cut(s)	491, 1824, 2190, 2580
SmlI	CTYRAG	4 cut(s)	212, 314, 1115, 1165
SmoI	CTYRAG	4 cut(s)	212, 314, 1115, 1165
SseBI	AGGCCT	1 cut(s)	2572
SspI	AATATT	2 cut(s)	2164, 3195
SspMI	CTAG	6 cut(s)	1539, 1659, 2151, 2381, 2495, 2789
StuI	AGGCCT	1 cut(s)	2572
StyD4I	CCNGG	9 cut(s)	435, 1805, 2301, 2438, 2476, 2487, 2859, 2903, 3176
StyI	CCWWGG	2 cut(s)	2667, 3425
TaiI	ACGT	8 cut(s)	209, 286, 506, 516, 1245, 1737, 2286, 3238
TaqII	GACCGA	1 cut(s)	3197
TatI	WGTACW	3 cut(s)	3, 2961, 3135
TauI	GCSGC	4 cut(s)	297, 418, 1055, 2277
TfiI	GAWTC	6 cut(s)	614, 1171, 1985, 2005, 2139, 2825
Tru1I	TTAA	6 cut(s)	203, 1088, 1166, 1265, 2333, 2912
Tru9I	TTAA	6 cut(s)	203, 1088, 1166, 1265, 2333, 2912
TscAI	CASTG	6 cut(s)	162, 1105, 2149, 3018, 3393, 3416
TseFI	GTSAC	8 cut(s)	52, 163, 184, 230, 279, 336, 2452, 3122
Tsp45I	GTSAC	8 cut(s)	52, 163, 184, 230, 279, 336, 2452, 3122
TspGWI	ACGGA	1 cut(s)	2698
TspRI	CASTG	6 cut(s)	162, 1105, 2149, 3018, 3393, 3416
Vha464I	CTTAAG	1 cut(s)	1165
VneI	GTGCAC	1 cut(s)	2886
VpaK11BI	GGWCC	6 cut(s)	424, 677, 704, 1000, 2644, 3180
XapI	RAATTY	7 cut(s)	979, 1514, 1857, 1940, 2530, 2724, 2732
XceI	RCATGY	4 cut(s)	461, 2430, 3003, 3409
XhoI	CTCGAG	1 cut(s)	212
XmiI	GTMKAC	2 cut(s)	1095, 2030
XmnI	GAANNNNTTC	3 cut(s)	697, 1331, 1789
XspI	CTAG	6 cut(s)	1539, 1659, 2151, 2381, 2495, 2789

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.