pycom15g04540

flavin-containing monooxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002931
Physical Location & Seq
Reverse (-)
2707345 .. 2709380
2036 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 726 bp
ATGAATTCGACCTCCGGGGAGTATGGTGGCCTTTTCAATGGCCATCTTGTTTGGGAGGTCGCTGTTCAGATGTCCAGCAATCCAAACATCATTCAGTGGTATGCATTCGAGTTCATCGTAGTTTGCATTGGAAAATATGGAGACATACCAAGAATGCCGAGTTTCCCGCGCAACAAAGGTCAAGAAGTATTTCAAGGCAAAGTCTTGCACTCCCTGGATTACTCTAAGCTTGACCAACGAGCGGCTCGTGAGCTCCTCAAAGGCAAAAAAGTTGCAGTTATTGGCTACAAGAAGTCAGCTATTGACGTGGCAGTTGAGTGCGCAGAGGCAAACCATGGACCCGATGGGCAGGCATGCACAATGGTGATAAGGACTCTACATTGGACAGTTCCATCTTACTGGATTTGGGGGCTTCCATTTTTCCTGTTTTTTTCGACAAGGTCTTCTCAATTCCTCCACGAAAGGCCAAACCAGAGCTTATTCCGAGCCTTGTTTTGCCTTCTTTCATCGCCTATGAGGATGGTAATTTCCAAGTTCATAGAATTGTACTTGGAGTGGAAGCTTCCCCTTGTGAAGTATGGACTAAAGCCTGATCGATCCATTTTTGGAGGACTATGCTTCATGCCAGATGGCTATTTTACCGGAAAATTTCTTCGCCGAGGCGGACAAGGGCAAAATATTGTTCAAAAGGTCATCAAAGTGGTGGTTTTGGAGCGGAGGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00982 ko01120 map00982 map01120
Pfam Domains
Protein Families
FMO-like

Protein Analysis

242

Amino Acids

27.53

Weight (kDa)

9.59

Isoelectric Point (pI)

38.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FMO-like PF00743 18 - 162 7.5e-10 Flavin-binding monooxygenase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 322
AccBSI CCGCTC 2 cut(s) 242, 715
AccII CGCG 1 cut(s) 169
AciI CCGC 4 cut(s) 167, 242, 663, 715
AclWI GGATC 1 cut(s) 591
AcoI YGGCCR 1 cut(s) 40
AcsI RAATTY 2 cut(s) 4, 647
AfaI GTAC 1 cut(s) 548
AfiI CCNNNNNNNGG 1 cut(s) 241
AgsI TTSAA 3 cut(s) 37, 194, 686
AjiI CACGTC 1 cut(s) 307
AjnI CCWGG 1 cut(s) 213
AleI CACNNNNGTG 1 cut(s) 362
AluBI AGCT 5 cut(s) 229, 253, 299, 477, 562
AluI AGCT 5 cut(s) 229, 253, 299, 477, 562
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 1 cut(s) 135
AlwI GGATC 1 cut(s) 591
AoxI GGCC 3 cut(s) 28, 40, 464
ApoI RAATTY 2 cut(s) 4, 647
Asp700I GAANNNNTTC 1 cut(s) 189
AspLEI GCGC 2 cut(s) 171, 323
AspS9I GGNCC 1 cut(s) 338
AsuC2I CCSGG 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 376
AvaII GGWCC 1 cut(s) 338
BalI TGGCCA 1 cut(s) 42
BanII GRGCYC 1 cut(s) 255
BauI CACGAG 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 435
Bbv12I GWGCWC 1 cut(s) 255
BccI CCATC 5 cut(s) 51, 338, 400, 514, 623
BciT130I CCWGG 1 cut(s) 215
BcnI CCSGG 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 135
BisI GCNGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 244
Bme1390I CCNGG 2 cut(s) 16, 215
Bme18I GGWCC 1 cut(s) 338
BmgBI CACGTC 1 cut(s) 307
BmgT120I GGNCC 1 cut(s) 338
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 2 cut(s) 16, 215
BpiI GAAGAC 1 cut(s) 435
BpuMI CCSGG 1 cut(s) 16
Bsa29I ATCGAT 1 cut(s) 595
BsaJI CCNNGG 4 cut(s) 15, 213, 334, 658
BsaWI WCCGGW 1 cut(s) 641
Bsc4I CCNNNNNNNGG 1 cut(s) 241
Bse1I ACTGG 1 cut(s) 404
BseBI CCWGG 1 cut(s) 215
BseCI ATCGAT 1 cut(s) 595
BseDI CCNNGG 4 cut(s) 15, 213, 334, 658
BseGI GGATG 1 cut(s) 525
BseLI CCNNNNNNNGG 1 cut(s) 241
BseNI ACTGG 1 cut(s) 404
BseRI GAGGAG 1 cut(s) 245
Bsh1236I CGCG 1 cut(s) 169
BshFI GGCC 3 cut(s) 30, 42, 466
BshVI ATCGAT 1 cut(s) 595
BsiHKAI GWGCWC 1 cut(s) 255
BsiSI CCGG 2 cut(s) 15, 642
BslI CCNNNNNNNGG 1 cut(s) 241
BsmAI GTCTC 1 cut(s) 135
BsmI GAATGC 2 cut(s) 104, 159
BsnI GGCC 3 cut(s) 30, 42, 466
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 2 cut(s) 592, 596
Bsp19I CCATGG 1 cut(s) 334
BspACI CCGC 4 cut(s) 167, 242, 663, 715
BspANI GGCC 3 cut(s) 30, 42, 466
BspDI ATCGAT 1 cut(s) 595
BspFNI CGCG 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 340
BspPI GGATC 1 cut(s) 591
BsrBI CCGCTC 2 cut(s) 242, 715
BsrI ACTGG 1 cut(s) 404
BssECI CCNNGG 4 cut(s) 15, 213, 334, 658
BssMI GATC 2 cut(s) 592, 596
BssSI CACGAG 1 cut(s) 246
BssT1I CCWWGG 1 cut(s) 334
Bst2BI CACGAG 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 1 cut(s) 388
BstC8I GCNNGC 2 cut(s) 351, 355
BstDEI CTNAG 1 cut(s) 225
BstDSI CCRYGG 1 cut(s) 334
BstF5I GGATG 1 cut(s) 525
BstFNI CGCG 1 cut(s) 169
BstHHI GCGC 2 cut(s) 171, 323
BstKTI GATC 2 cut(s) 595, 599
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 2 cut(s) 592, 596
BstNI CCWGG 1 cut(s) 215
BstNSI RCATGY 1 cut(s) 357
BstSCI CCNGG 2 cut(s) 14, 213
BstUI CGCG 1 cut(s) 169
BstV2I GAAGAC 1 cut(s) 435
BstXI CCANNNNNNTGG 1 cut(s) 399
Bsu15I ATCGAT 1 cut(s) 595
BsuRI GGCC 3 cut(s) 30, 42, 466
BsuTUI ATCGAT 1 cut(s) 595
BtgI CCRYGG 1 cut(s) 334
BtgZI GCGATG 1 cut(s) 492
BtrI CACGTC 1 cut(s) 307
BtsCI GGATG 1 cut(s) 525
BtsIMutI CAGTG 1 cut(s) 101
Cac8I GCNNGC 2 cut(s) 351, 355
CfoI GCGC 2 cut(s) 171, 323
Cfr13I GGNCC 1 cut(s) 338
ClaI ATCGAT 1 cut(s) 595
Csp6I GTAC 1 cut(s) 547
CviAII CATG 3 cut(s) 335, 354, 622
CviQI GTAC 1 cut(s) 547
DdeI CTNAG 1 cut(s) 225
DpnI GATC 2 cut(s) 594, 598
DpnII GATC 2 cut(s) 592, 596
EaeI YGGCCR 1 cut(s) 40
EciI GGCGGA 1 cut(s) 678
Ecl136II GAGCTC 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 334
Eco24I GRGCYC 1 cut(s) 255
Eco47I GGWCC 1 cut(s) 338
Eco53kI GAGCTC 1 cut(s) 253
EcoICRI GAGCTC 1 cut(s) 253
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 213
EcoT14I CCWWGG 1 cut(s) 334
EcoT22I ATGCAT 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 334
FaeI CATG 3 cut(s) 338, 357, 625
FatI CATG 3 cut(s) 334, 353, 621
FauI CCCGC 1 cut(s) 174
Fnu4HI GCNGC 1 cut(s) 243
FokI GGATG 1 cut(s) 532
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 1 cut(s) 243
FspI TGCGCA 1 cut(s) 322
GlaI GCGC 2 cut(s) 170, 322
GluI GCNGC 1 cut(s) 243
HaeIII GGCC 3 cut(s) 30, 42, 466
HapII CCGG 2 cut(s) 15, 642
HhaI GCGC 2 cut(s) 171, 323
Hin1II CATG 3 cut(s) 338, 357, 625
Hin6I GCGC 2 cut(s) 169, 321
HinP1I GCGC 2 cut(s) 169, 321
HindIII AAGCTT 2 cut(s) 227, 560
HinfI GANTC 1 cut(s) 373
HpaII CCGG 2 cut(s) 15, 642
HphI GGTGA 1 cut(s) 376
Hpy188I TCNGA 2 cut(s) 69, 485
Hpy188III TCNNGA 2 cut(s) 182, 248
HpyAV CCTTC 1 cut(s) 509
HpyCH4III ACNGT 1 cut(s) 388
HpyCH4IV ACGT 1 cut(s) 306
HpyCH4V TGCA 5 cut(s) 104, 126, 208, 275, 357
HpyF3I CTNAG 1 cut(s) 225
HpySE526I ACGT 1 cut(s) 306
Hsp92II CATG 3 cut(s) 338, 357, 625
HspAI GCGC 2 cut(s) 169, 321
Kzo9I GATC 2 cut(s) 592, 596
LmnI GCTCC 2 cut(s) 258, 712
MaeII ACGT 1 cut(s) 306
MalI GATC 2 cut(s) 594, 598
MbiI CCGCTC 2 cut(s) 242, 715
MboI GATC 2 cut(s) 592, 596
MboII GAAGA 2 cut(s) 435, 644
MhlI GDGCHC 1 cut(s) 255
MlsI TGGCCA 1 cut(s) 42
MluCI AATT 6 cut(s) 4, 449, 525, 542, 647, 721
MluNI TGGCCA 1 cut(s) 42
MlyI GAGTC 1 cut(s) 367
MnlI CCTC 9 cut(s) 22, 49, 266, 319, 464, 510, 602, 653, 711
Mox20I TGGCCA 1 cut(s) 42
Mph1103I ATGCAT 1 cut(s) 106
MroXI GAANNNNTTC 1 cut(s) 189
MscI TGGCCA 1 cut(s) 42
MslI CAYNNNNRTG 2 cut(s) 362, 698
Msp20I TGGCCA 1 cut(s) 42
MspI CCGG 2 cut(s) 15, 642
MspR9I CCNGG 2 cut(s) 16, 215
Mva1269I GAATGC 2 cut(s) 104, 159
MvaI CCWGG 1 cut(s) 215
MvnI CGCG 1 cut(s) 169
NciI CCSGG 1 cut(s) 16
NcoI CCATGG 1 cut(s) 334
NdeII GATC 2 cut(s) 592, 596
NlaIII CATG 3 cut(s) 338, 357, 625
NlaIV GGNNCC 1 cut(s) 340
NmeAIII GCCGAG 2 cut(s) 183, 683
NsbI TGCGCA 1 cut(s) 322
NsiI ATGCAT 1 cut(s) 106
NspI RCATGY 1 cut(s) 357
OliI CACNNNNGTG 1 cut(s) 362
PaeI GCATGC 1 cut(s) 357
PcsI WCGNNNNNNNCGW 2 cut(s) 114, 244
PctI GAATGC 2 cut(s) 104, 159
PdmI GAANNNNTTC 1 cut(s) 189
PflFI GACNNNGTC 1 cut(s) 439
PkrI GCNGC 1 cut(s) 244
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
Psp124BI GAGCTC 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 1 cut(s) 338
PsyI GACNNNGTC 1 cut(s) 439
RsaI GTAC 1 cut(s) 548
RsaNI GTAC 1 cut(s) 547
RseI CAYNNNNRTG 2 cut(s) 362, 698
SacI GAGCTC 1 cut(s) 255
SatI GCNGC 1 cut(s) 243
Sau3AI GATC 2 cut(s) 592, 596
Sau96I GGNCC 1 cut(s) 338
SchI GAGTC 1 cut(s) 367
ScrFI CCNGG 2 cut(s) 16, 215
SduI GDGCHC 1 cut(s) 255
SinI GGWCC 1 cut(s) 338
SmiMI CAYNNNNRTG 2 cut(s) 362, 698
SphI GCATGC 1 cut(s) 357
Sse9I AATT 6 cut(s) 4, 449, 525, 542, 647, 721
SsiI CCGC 4 cut(s) 167, 242, 663, 715
SspI AATATT 1 cut(s) 679
SstI GAGCTC 1 cut(s) 255
StyD4I CCNGG 2 cut(s) 14, 213
StyI CCWWGG 1 cut(s) 334
TaaI ACNGT 1 cut(s) 388
TaiI ACGT 1 cut(s) 309
TaqI TCGA 4 cut(s) 8, 108, 434, 595
TasI AATT 6 cut(s) 4, 449, 525, 542, 647, 721
TatI WGTACW 1 cut(s) 546
TauI GCSGC 1 cut(s) 245
TscAI CASTG 1 cut(s) 101
TspDTI ATGAA 5 cut(s) 17, 103, 495, 526, 610
TspRI CASTG 1 cut(s) 101
Tth111I GACNNNGTC 1 cut(s) 439
VpaK11BI GGWCC 1 cut(s) 338
XapI RAATTY 2 cut(s) 4, 647
XceI RCATGY 1 cut(s) 357
XcmI CCANNNNNNNNNTGG 1 cut(s) 341
XmnI GAANNNNTTC 1 cut(s) 189
Zsp2I ATGCAT 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.