pycom15g05960

branched-chain-amino-acid aminotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004977
Physical Location & Seq
Forward (+)
3626182 .. 3628694
2513 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1212 bp
ATGGCAATTTCCAATTCTACCCTCTCAATTGCTCTCCCATTCCCAACAACTCCCAGTTCATCCTCCTCGTCTCAGTCCCTCAAGCAGCAGCTTCTACTCGGGCCTTCTTGGCTTCGGTCGGCGGCTCCGATTGCCACCTCCACCACAAGCCTCAGAGCCGTCACTCCATCTGGTGTCGCTCATGATCAAACCTCTGAATTGCCTGATACAACATGGGACACGCTCGGATTCAACAAACCCATGTCGACAGATTACATGTTTGTTATGAAAAGTTCCAAAGATGGAGACTTTATGGATGGCGGGTTACAACGTTTCGAAAAAATTGAGTTCAACCCAGCTGCCTGTGTTTTGAACTATGGACAGGGAATAATTGAAGAATTGAAAGCATACAAGAAACATGACGGTTCTTTATTACTATTTCACCTAGAGGAACATGGGTTGCGGATGAGGACGGGCTCAGAGCGCCTGTGTATGCCTTCACCAACTGTTGAGCAGTTTGTGGAAGGTGTAAAAGCTACTGTCTTGGCAAATCGACGTTGGGTTCCCCCACGGAGTAAAGGCTTTCTTCATATTCGACCACTACTTATTGGGAATGGGCCTGTTCTTAGTCTTACACCAGCTCCAGAATTCACCTTTCTGATTTATGTTTCTCCAATGGGGAACTATTTTGAGGGGGGCTTGCAACCAATCAATTTGGTGGTAGAAAATGAAATCCATCGCGCAGTCCGTGGTGGAGCTGGAAGTGTGAAAGCCATAGGAAATTATAGTGCCATTTTGAAGGCACAGGTGGACGCTAAAGCAAATGGTTTCTCTGCTGTTTTGTACCTTGATTCTGTTCACAACAGATACATTGAAGAGACTTCTACTGCTAACATTTTTCTAGTGAAGGATAATGTAATCTCTACCCCGGCACTGCACGAAGGGACCATTTTGCCTGGCATTACCCGCAACTCTGTAATTGAAATTGCTCGCATCGAAGGGTACCAGGTTGAGGAACGCCTTGTGTCGTTTGAGGAATTATTTGAAGCCGATGAAGTATTTTGTACTGGAAATGCTGTCTTAACACCGGTGCGCAGCATAACTTACCTCGATAAAAGGGTGTCTTACCTTGAAACTGGCCCCGGTGGTGTCGTGTCTCAGAAACTGCACCGGGCGCTTTCAAATATACAGATGGGTCTTACAGAATTCGGCGGGACAGTTACCTTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00280 ko00290 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00270 map00280 map00290 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
Aminotran_4

Protein Analysis

404

Amino Acids

43.98

Weight (kDa)

6.17

Isoelectric Point (pI)

54.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_4 PF01063 125 - 360 7.3e-29 Amino-transferase class IV
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1073
Acc65I GGTACC 1 cut(s) 981
AccB1I GGYRCC 1 cut(s) 981
AccI GTMKAC 1 cut(s) 245
AccII CGCG 1 cut(s) 720
AciI CCGC 5 cut(s) 122, 300, 442, 946, 1191
AclI AACGTT 1 cut(s) 310
AcsI RAATTY 2 cut(s) 626, 1184
AfaI GTAC 3 cut(s) 824, 983, 1045
AfiI CCNNNNNNNGG 1 cut(s) 991
AflIII ACRYGT 1 cut(s) 255
AgeI ACCGGT 1 cut(s) 1066
AjnI CCWGG 2 cut(s) 934, 984
AluBI AGCT 5 cut(s) 91, 338, 515, 620, 737
AluI AGCT 5 cut(s) 91, 338, 515, 620, 737
Alw26I GTCTC 4 cut(s) 75, 279, 851, 1140
AlwNI CAGNNNCTG 1 cut(s) 1144
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 3 cut(s) 101, 596, 1117
ApeKI GCWGC 4 cut(s) 85, 88, 338, 1074
ApoI RAATTY 2 cut(s) 626, 1184
AsiGI ACCGGT 1 cut(s) 1066
Asp718I GGTACC 1 cut(s) 981
AspLEI GCGC 4 cut(s) 465, 722, 1074, 1156
AspS9I GGNCC 4 cut(s) 101, 596, 924, 1118
AsuC2I CCSGG 3 cut(s) 908, 1122, 1151
AsuHPI GGTGA 3 cut(s) 413, 471, 622
AsuII TTCGAA 1 cut(s) 315
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 1 cut(s) 924
BanI GGYRCC 1 cut(s) 981
BanII GRGCYC 1 cut(s) 458
BbvI GCAGC 4 cut(s) 97, 100, 325, 1086
BccI CCATC 5 cut(s) 175, 275, 290, 723, 1165
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 2 cut(s) 936, 986
BclI TGATCA 1 cut(s) 184
BcnI CCSGG 3 cut(s) 908, 1122, 1151
BcoDI GTCTC 4 cut(s) 75, 279, 851, 1140
BfaI CTAG 2 cut(s) 425, 881
BfoI RGCGCY 2 cut(s) 466, 1157
BglI GCCNNNNNGGC 1 cut(s) 109
BisI GCNGC 5 cut(s) 86, 89, 123, 339, 1075
BlsI GCNGC 5 cut(s) 87, 90, 124, 340, 1076
Bme1390I CCNGG 5 cut(s) 908, 936, 986, 1122, 1151
Bme18I GGWCC 1 cut(s) 924
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 4 cut(s) 101, 596, 924, 1118
BmiI GGNNCC 5 cut(s) 126, 543, 925, 983, 1120
BmrFI CCNGG 5 cut(s) 908, 936, 986, 1122, 1151
BmrI ACTGGG 1 cut(s) 48
BmsI GCATC 1 cut(s) 981
BmuI ACTGGG 1 cut(s) 48
BpmI CTGGAG 1 cut(s) 606
Bpu14I TTCGAA 1 cut(s) 315
BpuEI CTTGAG 1 cut(s) 65
BpuMI CCSGG 3 cut(s) 908, 1122, 1151
BsaJI CCNNGG 4 cut(s) 548, 727, 906, 1120
BsaWI WCCGGW 1 cut(s) 1066
BsaXI ACNNNNNCTCC 2 cut(s) 604, 634
Bsc4I CCNNNNNNNGG 1 cut(s) 991
Bse118I RCCGGY 1 cut(s) 1066
Bse1I ACTGG 3 cut(s) 54, 1051, 1120
BseBI CCWGG 2 cut(s) 936, 986
BseDI CCNNGG 4 cut(s) 548, 727, 906, 1120
BseGI GGATG 3 cut(s) 59, 301, 450
BseLI CCNNNNNNNGG 1 cut(s) 991
BseMII CTCAG 4 cut(s) 86, 166, 471, 1151
BseNI ACTGG 3 cut(s) 54, 1051, 1120
BseRI GAGGAG 1 cut(s) 55
BseXI GCAGC 4 cut(s) 97, 100, 325, 1086
BseYI CCCAGC 1 cut(s) 334
BsgI GTGCAG 2 cut(s) 899, 1130
Bsh1236I CGCG 1 cut(s) 720
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 3 cut(s) 103, 598, 1119
BshNI GGYRCC 1 cut(s) 981
BshTI ACCGGT 1 cut(s) 1066
BsiEI CGRYCG 1 cut(s) 119
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 4 cut(s) 908, 1067, 1122, 1150
BslFI GGGAC 4 cut(s) 61, 230, 937, 1207
BslI CCNNNNNNNGG 1 cut(s) 991
BsmAI GTCTC 4 cut(s) 75, 279, 851, 1140
BsmBI CGTCTC 1 cut(s) 75
BsmFI GGGAC 4 cut(s) 61, 230, 937, 1207
BsnI GGCC 3 cut(s) 103, 598, 1119
BsoBI CYCGRG 1 cut(s) 98
Bsp119I TTCGAA 1 cut(s) 315
Bsp1286I GDGCHC 1 cut(s) 458
Bsp143I GATC 1 cut(s) 184
BspACI CCGC 5 cut(s) 122, 300, 442, 946, 1191
BspANI GGCC 3 cut(s) 103, 598, 1119
BspCNI CTCAG 4 cut(s) 85, 165, 470, 1150
BspFNI CGCG 1 cut(s) 720
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 5 cut(s) 126, 543, 925, 983, 1120
BspT104I TTCGAA 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 981
BsrFI RCCGGY 1 cut(s) 1066
BsrI ACTGG 3 cut(s) 54, 1051, 1120
BssAI RCCGGY 1 cut(s) 1066
BssECI CCNNGG 4 cut(s) 548, 727, 906, 1120
BssMI GATC 1 cut(s) 184
Bst2UI CCWGG 2 cut(s) 936, 986
Bst4CI ACNGT 4 cut(s) 404, 487, 520, 1198
Bst6I CTCTTC 1 cut(s) 849
BstBI TTCGAA 1 cut(s) 315
BstC8I GCNNGC 2 cut(s) 680, 970
BstDEI CTNAG 5 cut(s) 72, 152, 457, 605, 1137
BstDSI CCRYGG 2 cut(s) 548, 727
BstF5I GGATG 3 cut(s) 59, 301, 450
BstFNI CGCG 1 cut(s) 720
BstH2I RGCGCY 2 cut(s) 466, 1157
BstHHI GCGC 4 cut(s) 465, 722, 1074, 1156
BstKTI GATC 1 cut(s) 187
BstMAI GTCTC 4 cut(s) 75, 279, 851, 1140
BstMBI GATC 1 cut(s) 184
BstMCI CGRYCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 5 cut(s) 109, 131, 462, 945, 1153
BstNI CCWGG 2 cut(s) 936, 986
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 5 cut(s) 906, 934, 984, 1120, 1149
BstUI CGCG 1 cut(s) 720
BstV1I GCAGC 4 cut(s) 97, 100, 325, 1086
BsuRI GGCC 3 cut(s) 103, 598, 1119
BtgI CCRYGG 2 cut(s) 548, 727
BtgZI GCGATG 1 cut(s) 701
BtsCI GGATG 3 cut(s) 59, 301, 450
BtsI GCAGTG 1 cut(s) 911
BtsIMutI CAGTG 1 cut(s) 911
Cac8I GCNNGC 2 cut(s) 680, 970
CaiI CAGNNNCTG 1 cut(s) 1144
CciI TCATGA 1 cut(s) 181
CfoI GCGC 4 cut(s) 465, 722, 1074, 1156
Cfr10I RCCGGY 1 cut(s) 1066
Cfr13I GGNCC 4 cut(s) 101, 596, 924, 1118
CseI GACGC 1 cut(s) 800
CsiI ACCWGGT 1 cut(s) 984
Csp6I GTAC 3 cut(s) 823, 982, 1044
CspAI ACCGGT 1 cut(s) 1066
CviAII CATG 6 cut(s) 182, 213, 241, 256, 398, 434
CviQI GTAC 3 cut(s) 823, 982, 1044
DdeI CTNAG 5 cut(s) 72, 152, 457, 605, 1137
DpnI GATC 1 cut(s) 186
DpnII GATC 1 cut(s) 184
Eam1104I CTCTTC 1 cut(s) 849
EarI CTCTTC 1 cut(s) 849
Eco24I GRGCYC 1 cut(s) 458
Eco47I GGWCC 1 cut(s) 924
Eco88I CYCGRG 1 cut(s) 98
EcoRI GAATTC 2 cut(s) 626, 1184
EcoRII CCWGG 2 cut(s) 934, 984
EcoT38I GRGCYC 1 cut(s) 458
Esp3I CGTCTC 1 cut(s) 75
FaeI CATG 6 cut(s) 185, 216, 244, 259, 401, 437
FalI AAGNNNNNCTT 4 cut(s) 549, 581, 1087, 1119
FaqI GGGAC 4 cut(s) 61, 230, 937, 1207
FatI CATG 6 cut(s) 181, 212, 240, 255, 397, 433
FauI CCCGC 3 cut(s) 293, 953, 1184
FbaI TGATCA 1 cut(s) 184
FblI GTMKAC 1 cut(s) 245
Fnu4HI GCNGC 5 cut(s) 86, 89, 123, 339, 1075
FokI GGATG 3 cut(s) 46, 308, 457
FriOI GRGCYC 1 cut(s) 458
Fsp4HI GCNGC 5 cut(s) 86, 89, 123, 339, 1075
FspBI CTAG 2 cut(s) 425, 881
FspI TGCGCA 1 cut(s) 1073
GlaI GCGC 4 cut(s) 464, 721, 1073, 1155
GluI GCNGC 5 cut(s) 86, 89, 123, 339, 1075
GsaI CCCAGC 1 cut(s) 338
GsuI CTGGAG 1 cut(s) 606
HaeII RGCGCY 2 cut(s) 466, 1157
HaeIII GGCC 3 cut(s) 103, 598, 1119
HapII CCGG 4 cut(s) 908, 1067, 1122, 1150
HgaI GACGC 1 cut(s) 800
HhaI GCGC 4 cut(s) 465, 722, 1074, 1156
Hin1II CATG 6 cut(s) 185, 216, 244, 259, 401, 437
Hin6I GCGC 4 cut(s) 463, 720, 1072, 1154
HinP1I GCGC 4 cut(s) 463, 720, 1072, 1154
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HinfI GANTC 2 cut(s) 228, 830
HpaII CCGG 4 cut(s) 908, 1067, 1122, 1150
HphI GGTGA 3 cut(s) 413, 471, 622
Hpy166II GTNNAC 3 cut(s) 246, 790, 838
Hpy188I TCNGA 7 cut(s) 129, 155, 196, 227, 460, 639, 1140
Hpy188III TCNNGA 2 cut(s) 182, 623
Hpy8I GTNNAC 3 cut(s) 246, 790, 838
Hpy99I CGWCG 1 cut(s) 537
HpyAV CCTTC 7 cut(s) 114, 486, 497, 772, 880, 914, 971
HpyCH4III ACNGT 4 cut(s) 404, 487, 520, 1198
HpyCH4IV ACGT 2 cut(s) 310, 535
HpyCH4V TGCA 3 cut(s) 682, 916, 1147
HpyF10VI GCNNNNNNNGC 5 cut(s) 109, 131, 462, 945, 1153
HpyF3I CTNAG 5 cut(s) 72, 152, 457, 605, 1137
HpySE526I ACGT 2 cut(s) 310, 535
Hsp92II CATG 6 cut(s) 185, 216, 244, 259, 401, 437
HspAI GCGC 4 cut(s) 463, 720, 1072, 1154
KpnI GGTACC 1 cut(s) 985
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 1 cut(s) 184
LmnI GCTCC 3 cut(s) 130, 625, 734
Lsp1109I GCAGC 4 cut(s) 97, 100, 325, 1086
LweI GCATC 1 cut(s) 981
MabI ACCWGGT 1 cut(s) 984
MaeI CTAG 2 cut(s) 425, 881
MaeII ACGT 2 cut(s) 310, 535
MaeIII GTNAC 3 cut(s) 160, 303, 1198
MalI GATC 1 cut(s) 186
MboI GATC 1 cut(s) 184
MboII GAAGA 3 cut(s) 386, 557, 866
MfeI CAATTG 1 cut(s) 27
MhlI GDGCHC 1 cut(s) 458
MseI TTAA 1 cut(s) 1061
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 4 cut(s) 908, 1067, 1122, 1150
MspR9I CCNGG 5 cut(s) 908, 936, 986, 1122, 1151
MunI CAATTG 1 cut(s) 27
MvaI CCWGG 2 cut(s) 936, 986
MvnI CGCG 1 cut(s) 720
MwoI GCNNNNNNNGC 5 cut(s) 109, 131, 462, 945, 1153
NciI CCSGG 3 cut(s) 908, 1122, 1151
NdeII GATC 1 cut(s) 184
NlaIII CATG 6 cut(s) 185, 216, 244, 259, 401, 437
NlaIV GGNNCC 5 cut(s) 126, 543, 925, 983, 1120
NmuCI GTSAC 1 cut(s) 160
NsbI TGCGCA 1 cut(s) 1073
NspI RCATGY 1 cut(s) 259
NspV TTCGAA 1 cut(s) 315
PagI TCATGA 1 cut(s) 181
PciI ACATGT 1 cut(s) 255
PcsI WCGNNNNNNNCGW 2 cut(s) 125, 724
PfeI GAWTC 2 cut(s) 228, 830
PinAI ACCGGT 1 cut(s) 1066
PkrI GCNGC 5 cut(s) 87, 90, 124, 340, 1076
PscI ACATGT 1 cut(s) 255
Psp1406I AACGTT 1 cut(s) 310
Psp6I CCWGG 2 cut(s) 934, 984
PspFI CCCAGC 1 cut(s) 334
PspGI CCWGG 2 cut(s) 934, 984
PspN4I GGNNCC 5 cut(s) 126, 543, 925, 983, 1120
PspPI GGNCC 4 cut(s) 101, 596, 924, 1118
PstNI CAGNNNCTG 1 cut(s) 1144
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 3 cut(s) 824, 983, 1045
RsaNI GTAC 3 cut(s) 823, 982, 1044
SalI GTCGAC 1 cut(s) 244
SaqAI TTAA 1 cut(s) 1061
SatI GCNGC 5 cut(s) 86, 89, 123, 339, 1075
Sau3AI GATC 1 cut(s) 184
Sau96I GGNCC 4 cut(s) 101, 596, 924, 1118
ScrFI CCNGG 5 cut(s) 908, 936, 986, 1122, 1151
SduI GDGCHC 1 cut(s) 458
SexAI ACCWGGT 1 cut(s) 984
SfaNI GCATC 1 cut(s) 981
SfuI TTCGAA 1 cut(s) 315
SgrAI CRCCGGYG 1 cut(s) 1066
SinI GGWCC 1 cut(s) 924
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
SsiI CCGC 5 cut(s) 122, 300, 442, 946, 1191
SspMI CTAG 2 cut(s) 425, 881
StyD4I CCNGG 5 cut(s) 906, 934, 984, 1120, 1149
TaaI ACNGT 4 cut(s) 404, 487, 520, 1198
TaiI ACGT 2 cut(s) 313, 538
TaqI TCGA 6 cut(s) 245, 315, 532, 574, 975, 1089
TaqII GACCGA 1 cut(s) 105
TatI WGTACW 1 cut(s) 1043
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 2 cut(s) 228, 830
Tru1I TTAA 1 cut(s) 1061
Tru9I TTAA 1 cut(s) 1061
TscAI CASTG 1 cut(s) 918
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 4 cut(s) 85, 88, 338, 1074
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 5 cut(s) 48, 281, 557, 723, 1047
TspGWI ACGGA 2 cut(s) 565, 716
TspRI CASTG 1 cut(s) 918
VpaK11BI GGWCC 1 cut(s) 924
XapI RAATTY 2 cut(s) 626, 1184
XceI RCATGY 1 cut(s) 259
XmiI GTMKAC 1 cut(s) 245
XspI CTAG 2 cut(s) 425, 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.