pycom15g14840

Clathrin assembly protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0004817
Physical Location & Seq
Forward (+)
10180395 .. 10183112
2718 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1230 bp
ATGCATGGGATTATTCTACCTGGATCCGTCCGTTATGCCTTGTTCTTGGAGGAGAGACTGGAATGCTTTCGTGTGTTGAAATATGACGTTGAGATGGATCGCCCAAGGACCAAAGATCTGGATACTGCCGAATTGCTTGAGCATTTGCCTGCCCTACAACAGCTTCTCTTTCGTGTACTTGGTTGCCAGCCGCAAGGGGCAGCAGTCCATAATTTTGTGGTTCAGTTAGCACTTTCAATGGTTGCTTCTGAAAGCATCAAGATTTATCAAGCTATAAGTGATGGAACAGTCAATTTGGTTGACAAGTTCTTTGAGATGCAACGCCATGACGCTATGAGGGCCCTGGATATATATCGGAGGGCAGGGCAGCAGGCAGAGAGACTGTCAGAGTTTTATGAAGTATGTAAAAGTCTTGATATTGGGCGCGGAGAAAGGTTTATTAAGATTGAGCAGCCTCCTGCATCGTTTTTACAAGCCATGGAAGAGTATGTGAAAGAAGCTCCCCGGATCTCAACAGTTCGCAAGGATCAGGTGGTTGCCCCCAAAGAAATCTTGGCTATAGAGTACAAAAAGGCCCCAGAGGAGGAGGCGCGTCCACCTTCACCACCTCCACCTGAACCAGTGAAAGTTGAACCAGTGAAAGTGGAAGCGCCTGTAGCTGAACAGCCTGATTTGCTGGGTTTGAATGATCCTGTTCCAAATACCAAAGAGTTAGATGACAAGAATGCACTGGCCTTAGCGATTGTTCCAGTTTCAGACCAACCAACTTCCACGGCTCCAACTCTAGCAAATGGAGCTACAGGTTGGGAATTGGCACTTGTTACAGCACCGAGCTCAAATGAGAGTGCTGTGGCTTCTAGCAAACTGGCGGGAGGGTTAGACTTGCTTACACTAGACAGCCTATATGACGATGCAATCAGACGAAGCAATCAGAATGTGAGCCACAACCCATGGGAGCCTGCTCCAATGACTGGTGCCATGATGCAACAACCAGTTCATGACCCCTTTTATGCATCCAACAGAATGGCTGCACCGCATTCTGTGCAGATGGCAGCAATGGCAAACCAGCAGCAGGCCTTCATGTTGCAGCAACAGCAACAGCAGATGATGATGGGTCAACAACAGCAGATGTCTTCGAATCCTTTCGGAACTCCTCATGGAGCCCCCACCCACCCCTATGGCCCCGGCGTCCCTGTCCAAGCATACAACCCATATACAGGCCTTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANTH

Protein Analysis

410

Amino Acids

45.19

Weight (kDa)

5.16

Isoelectric Point (pI)

55.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANTH PF07651 11 - 165 1.1e-45 ANTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1228
AccB1I GGYRCC 1 cut(s) 974
AccB7I CCANNNNNTGG 1 cut(s) 971
AccII CGCG 2 cut(s) 426, 592
AciI CCGC 4 cut(s) 191, 426, 869, 1034
AclWI GGATC 6 cut(s) 18, 31, 105, 515, 534, 683
AcyI GRCGYC 1 cut(s) 1188
AfaI GTAC 2 cut(s) 177, 566
AfiI CCNNNNNNNGG 4 cut(s) 583, 971, 1072, 1217
AgsI TTSAA 4 cut(s) 79, 237, 632, 685
AjnI CCWGG 2 cut(s) 19, 342
AluBI AGCT 6 cut(s) 163, 272, 500, 659, 797, 834
AluI AGCT 6 cut(s) 163, 272, 500, 659, 797, 834
Alw21I GWGCWC 1 cut(s) 836
Alw26I GTCTC 2 cut(s) 49, 373
AlwI GGATC 6 cut(s) 18, 31, 105, 515, 534, 683
AoxI GGCC 6 cut(s) 339, 573, 732, 1074, 1180, 1219
ApaI GGGCCC 1 cut(s) 343
ApeKI GCWGC 7 cut(s) 200, 367, 451, 1028, 1052, 1069, 1087
ArsI GACNNNNNNTTYG 2 cut(s) 293, 325
Asp700I GAANNNNTTC 2 cut(s) 66, 1142
AspLEI GCGC 3 cut(s) 426, 592, 652
AspS9I GGNCC 5 cut(s) 108, 339, 340, 574, 1181
AsuC2I CCSGG 2 cut(s) 505, 1185
AsuHPI GGTGA 1 cut(s) 594
AsuII TTCGAA 1 cut(s) 1136
AvaII GGWCC 1 cut(s) 108
BaeGI GKGCMC 1 cut(s) 343
BamHI GGATCC 1 cut(s) 23
BanI GGYRCC 1 cut(s) 974
BanII GRGCYC 3 cut(s) 343, 836, 1165
BbsI GAAGAC 1 cut(s) 1125
Bbv12I GWGCWC 1 cut(s) 836
BbvI GCAGC 7 cut(s) 212, 379, 463, 1015, 1064, 1081, 1099
BccI CCATC 4 cut(s) 88, 275, 1042, 1105
BceAI ACGGC 1 cut(s) 789
BciT130I CCWGG 2 cut(s) 21, 344
BciVI GTATCC 1 cut(s) 115
BcnI CCSGG 2 cut(s) 505, 1185
BcoDI GTCTC 2 cut(s) 49, 373
BfaI CTAG 3 cut(s) 785, 858, 893
BfmI CTRYAG 3 cut(s) 558, 654, 798
BfoI RGCGCY 1 cut(s) 653
BfuI GTATCC 1 cut(s) 115
BglII AGATCT 1 cut(s) 115
BisI GCNGC 8 cut(s) 191, 201, 368, 452, 1029, 1053, 1070, 1088
BlsI GCNGC 8 cut(s) 192, 202, 369, 453, 1030, 1054, 1071, 1089
Bme1390I CCNGG 4 cut(s) 21, 344, 505, 1185
Bme18I GGWCC 1 cut(s) 108
BmgT120I GGNCC 5 cut(s) 108, 339, 340, 574, 1181
BmiI GGNNCC 8 cut(s) 25, 341, 576, 777, 957, 976, 1162, 1183
BmrFI CCNGG 4 cut(s) 21, 344, 505, 1185
BmsI GCATC 6 cut(s) 264, 306, 470, 901, 972, 1022
BpiI GAAGAC 1 cut(s) 1125
Bpu10I CCTNAGC 1 cut(s) 736
Bpu14I TTCGAA 1 cut(s) 1136
BpuEI CTTGAG 1 cut(s) 158
BpuMI CCSGG 2 cut(s) 505, 1185
BsaBI GATNNNNATC 1 cut(s) 351
BsaHI GRCGYC 1 cut(s) 1188
BsaJI CCNNGG 7 cut(s) 104, 342, 477, 503, 771, 950, 1183
Bsc4I CCNNNNNNNGG 4 cut(s) 583, 971, 1072, 1217
Bse1I ACTGG 8 cut(s) 63, 620, 635, 735, 749, 870, 976, 992
Bse3DI GCAATG 1 cut(s) 1062
Bse8I GATNNNNATC 1 cut(s) 351
BseBI CCWGG 2 cut(s) 21, 344
BseDI CCNNGG 7 cut(s) 104, 342, 477, 503, 771, 950, 1183
BseGI GGATG 1 cut(s) 1013
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 4 cut(s) 583, 971, 1072, 1217
BseMI GCAATG 1 cut(s) 1062
BseNI ACTGG 8 cut(s) 63, 620, 635, 735, 749, 870, 976, 992
BseRI GAGGAG 4 cut(s) 65, 596, 599, 1143
BseSI GKGCMC 1 cut(s) 343
BseXI GCAGC 7 cut(s) 212, 379, 463, 1015, 1064, 1081, 1099
BseYI CCCAGC 1 cut(s) 676
BsgI GTGCAG 2 cut(s) 1014, 1064
Bsh1236I CGCG 2 cut(s) 426, 592
BshFI GGCC 6 cut(s) 341, 575, 734, 1076, 1182, 1221
BshNI GGYRCC 1 cut(s) 974
BsiHKAI GWGCWC 1 cut(s) 836
BsiSI CCGG 2 cut(s) 505, 1185
BslFI GGGAC 1 cut(s) 1175
BslI CCNNNNNNNGG 4 cut(s) 583, 971, 1072, 1217
BsmAI GTCTC 2 cut(s) 49, 373
BsmFI GGGAC 1 cut(s) 1175
BsmI GAATGC 3 cut(s) 68, 730, 1036
BsnI GGCC 6 cut(s) 341, 575, 734, 1076, 1182, 1221
Bsp119I TTCGAA 1 cut(s) 1136
Bsp120I GGGCCC 1 cut(s) 339
Bsp1286I GDGCHC 3 cut(s) 343, 836, 1165
Bsp143I GATC 6 cut(s) 23, 97, 115, 507, 526, 688
Bsp19I CCATGG 2 cut(s) 477, 950
BspACI CCGC 4 cut(s) 191, 426, 869, 1034
BspANI GGCC 6 cut(s) 341, 575, 734, 1076, 1182, 1221
BspFNI CGCG 2 cut(s) 426, 592
BspHI TCATGA 1 cut(s) 997
BspLI GGNNCC 8 cut(s) 25, 341, 576, 777, 957, 976, 1162, 1183
BspPI GGATC 6 cut(s) 18, 31, 105, 515, 534, 683
BspT104I TTCGAA 1 cut(s) 1136
BspT107I GGYRCC 1 cut(s) 974
BsrDI GCAATG 1 cut(s) 1062
BsrI ACTGG 8 cut(s) 63, 620, 635, 735, 749, 870, 976, 992
BssECI CCNNGG 7 cut(s) 104, 342, 477, 503, 771, 950, 1183
BssMI GATC 6 cut(s) 23, 97, 115, 507, 526, 688
BssNI GRCGYC 1 cut(s) 1188
BssT1I CCWWGG 3 cut(s) 104, 477, 950
Bst2UI CCWGG 2 cut(s) 21, 344
Bst4CI ACNGT 3 cut(s) 289, 384, 517
Bst6I CTCTTC 1 cut(s) 477
BstACI GRCGYC 1 cut(s) 1188
BstAPI GCANNNNNTGC 1 cut(s) 1042
BstBI TTCGAA 1 cut(s) 1136
BstC8I GCNNGC 5 cut(s) 150, 188, 372, 960, 1074
BstDEI CTNAG 1 cut(s) 736
BstDSI CCRYGG 3 cut(s) 477, 771, 950
BstF5I GGATG 1 cut(s) 1013
BstFNI CGCG 2 cut(s) 426, 592
BstH2I RGCGCY 1 cut(s) 653
BstHHI GCGC 3 cut(s) 426, 592, 652
BstKTI GATC 6 cut(s) 26, 100, 118, 510, 529, 691
BstMAI GTCTC 2 cut(s) 49, 373
BstMBI GATC 6 cut(s) 23, 97, 115, 507, 526, 688
BstMWI GCNNNNNNNGC 7 cut(s) 338, 656, 673, 794, 1042, 1058, 1093
BstNI CCWGG 2 cut(s) 21, 344
BstSCI CCNGG 4 cut(s) 19, 342, 503, 1183
BstSFI CTRYAG 3 cut(s) 558, 654, 798
BstSLI GKGCMC 1 cut(s) 343
BstUI CGCG 2 cut(s) 426, 592
BstV1I GCAGC 7 cut(s) 212, 379, 463, 1015, 1064, 1081, 1099
BstV2I GAAGAC 1 cut(s) 1125
BstX2I RGATCY 3 cut(s) 23, 115, 507
BstXI CCANNNNNNTGG 3 cut(s) 118, 1024, 1178
BstYI RGATCY 3 cut(s) 23, 115, 507
BsuI GTATCC 1 cut(s) 115
BsuRI GGCC 6 cut(s) 341, 575, 734, 1076, 1182, 1221
BtgI CCRYGG 3 cut(s) 477, 771, 950
BtsCI GGATG 1 cut(s) 1013
BtsIMutI CAGTG 3 cut(s) 627, 642, 728
Cac8I GCNNGC 5 cut(s) 150, 188, 372, 960, 1074
CciI TCATGA 1 cut(s) 997
CfoI GCGC 3 cut(s) 426, 592, 652
Cfr13I GGNCC 5 cut(s) 108, 339, 340, 574, 1181
CseI GACGC 3 cut(s) 338, 581, 1177
Csp6I GTAC 2 cut(s) 176, 565
CviAII CATG 8 cut(s) 5, 326, 478, 951, 979, 998, 1081, 1157
CviQI GTAC 2 cut(s) 176, 565
DdeI CTNAG 1 cut(s) 736
DpnI GATC 6 cut(s) 25, 99, 117, 509, 528, 690
DpnII GATC 6 cut(s) 23, 97, 115, 507, 526, 688
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Ecl136II GAGCTC 1 cut(s) 834
Eco130I CCWWGG 3 cut(s) 104, 477, 950
Eco147I AGGCCT 2 cut(s) 1076, 1221
Eco24I GRGCYC 3 cut(s) 343, 836, 1165
Eco47I GGWCC 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 834
EcoICRI GAGCTC 1 cut(s) 834
EcoO109I RGGNCCY 3 cut(s) 339, 340, 574
EcoRII CCWGG 2 cut(s) 19, 342
EcoT14I CCWWGG 3 cut(s) 104, 477, 950
EcoT22I ATGCAT 2 cut(s) 6, 1015
EcoT38I GRGCYC 3 cut(s) 343, 836, 1165
ErhI CCWWGG 3 cut(s) 104, 477, 950
FaeI CATG 8 cut(s) 8, 329, 481, 954, 982, 1001, 1084, 1160
FaqI GGGAC 1 cut(s) 1175
FatI CATG 8 cut(s) 4, 325, 477, 950, 978, 997, 1080, 1156
FauI CCCGC 1 cut(s) 862
Fnu4HI GCNGC 8 cut(s) 191, 201, 368, 452, 1029, 1053, 1070, 1088
FokI GGATG 1 cut(s) 1000
FriOI GRGCYC 3 cut(s) 343, 836, 1165
Fsp4HI GCNGC 8 cut(s) 191, 201, 368, 452, 1029, 1053, 1070, 1088
FspBI CTAG 3 cut(s) 785, 858, 893
GlaI GCGC 3 cut(s) 425, 591, 651
GluI GCNGC 8 cut(s) 191, 201, 368, 452, 1029, 1053, 1070, 1088
GsaI CCCAGC 1 cut(s) 680
HaeII RGCGCY 1 cut(s) 653
HaeIII GGCC 6 cut(s) 341, 575, 734, 1076, 1182, 1221
HapII CCGG 2 cut(s) 505, 1185
HgaI GACGC 3 cut(s) 338, 581, 1177
HhaI GCGC 3 cut(s) 426, 592, 652
Hin1I GRCGYC 1 cut(s) 1188
Hin1II CATG 8 cut(s) 8, 329, 481, 954, 982, 1001, 1084, 1160
Hin6I GCGC 3 cut(s) 424, 590, 650
HinP1I GCGC 3 cut(s) 424, 590, 650
HincII GTYRAC 2 cut(s) 301, 1118
HindII GTYRAC 2 cut(s) 301, 1118
HinfI GANTC 1 cut(s) 1138
HpaII CCGG 2 cut(s) 505, 1185
HphI GGTGA 1 cut(s) 594
Hpy166II GTNNAC 4 cut(s) 176, 301, 596, 1118
Hpy188I TCNGA 7 cut(s) 250, 357, 388, 757, 920, 933, 1148
Hpy188III TCNNGA 4 cut(s) 119, 259, 413, 998
Hpy8I GTNNAC 4 cut(s) 176, 301, 596, 1118
HpyAV CCTTC 2 cut(s) 609, 1087
HpyCH4III ACNGT 3 cut(s) 289, 384, 517
HpyCH4IV ACGT 1 cut(s) 87
HpyF10VI GCNNNNNNNGC 7 cut(s) 338, 656, 673, 794, 1042, 1058, 1093
HpyF3I CTNAG 1 cut(s) 736
HpySE526I ACGT 1 cut(s) 87
Hsp92I GRCGYC 1 cut(s) 1188
Hsp92II CATG 8 cut(s) 8, 329, 481, 954, 982, 1001, 1084, 1160
HspAI GCGC 3 cut(s) 424, 590, 650
Kzo9I GATC 6 cut(s) 23, 97, 115, 507, 526, 688
LmnI GCTCC 6 cut(s) 505, 781, 794, 955, 967, 1160
Lsp1109I GCAGC 7 cut(s) 212, 379, 463, 1015, 1064, 1081, 1099
LweI GCATC 6 cut(s) 264, 306, 470, 901, 972, 1022
MaeI CTAG 3 cut(s) 785, 858, 893
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 1 cut(s) 820
MalI GATC 6 cut(s) 25, 99, 117, 509, 528, 690
MboI GATC 6 cut(s) 23, 97, 115, 507, 526, 688
MboII GAAGA 2 cut(s) 494, 1125
MflI RGATCY 3 cut(s) 23, 115, 507
MhlI GDGCHC 3 cut(s) 343, 836, 1165
MluCI AATT 4 cut(s) 131, 211, 292, 809
MmeI TCCRAC 2 cut(s) 803, 1041
Mph1103I ATGCAT 2 cut(s) 6, 1015
MroXI GAANNNNTTC 2 cut(s) 66, 1142
MseI TTAA 1 cut(s) 441
MslI CAYNNNNRTG 1 cut(s) 1176
MspI CCGG 2 cut(s) 505, 1185
MspR9I CCNGG 4 cut(s) 21, 344, 505, 1185
Mva1269I GAATGC 3 cut(s) 68, 730, 1036
MvaI CCWGG 2 cut(s) 21, 344
MvnI CGCG 2 cut(s) 426, 592
MwoI GCNNNNNNNGC 7 cut(s) 338, 656, 673, 794, 1042, 1058, 1093
NciI CCSGG 2 cut(s) 505, 1185
NcoI CCATGG 2 cut(s) 477, 950
NdeII GATC 6 cut(s) 23, 97, 115, 507, 526, 688
NlaIII CATG 8 cut(s) 8, 329, 481, 954, 982, 1001, 1084, 1160
NlaIV GGNNCC 8 cut(s) 25, 341, 576, 777, 957, 976, 1162, 1183
NsiI ATGCAT 2 cut(s) 6, 1015
NspV TTCGAA 1 cut(s) 1136
PagI TCATGA 1 cut(s) 997
PceI AGGCCT 2 cut(s) 1076, 1221
PctI GAATGC 3 cut(s) 68, 730, 1036
PdmI GAANNNNTTC 2 cut(s) 66, 1142
PfeI GAWTC 1 cut(s) 1138
PflMI CCANNNNNTGG 1 cut(s) 971
PkrI GCNGC 8 cut(s) 192, 202, 369, 453, 1030, 1054, 1071, 1089
PsiI TTATAA 1 cut(s) 1228
Psp124BI GAGCTC 1 cut(s) 836
Psp6I CCWGG 2 cut(s) 19, 342
PspFI CCCAGC 1 cut(s) 676
PspGI CCWGG 2 cut(s) 19, 342
PspN4I GGNNCC 8 cut(s) 25, 341, 576, 777, 957, 976, 1162, 1183
PspOMI GGGCCC 1 cut(s) 339
PspPI GGNCC 5 cut(s) 108, 339, 340, 574, 1181
PsuI RGATCY 3 cut(s) 23, 115, 507
RsaI GTAC 2 cut(s) 177, 566
RsaNI GTAC 2 cut(s) 176, 565
RseI CAYNNNNRTG 1 cut(s) 1176
SacI GAGCTC 1 cut(s) 836
SaqAI TTAA 1 cut(s) 441
SatI GCNGC 8 cut(s) 191, 201, 368, 452, 1029, 1053, 1070, 1088
Sau3AI GATC 6 cut(s) 23, 97, 115, 507, 526, 688
Sau96I GGNCC 5 cut(s) 108, 339, 340, 574, 1181
ScrFI CCNGG 4 cut(s) 21, 344, 505, 1185
SduI GDGCHC 3 cut(s) 343, 836, 1165
SfaNI GCATC 6 cut(s) 264, 306, 470, 901, 972, 1022
SfcI CTRYAG 3 cut(s) 558, 654, 798
SfuI TTCGAA 1 cut(s) 1136
SinI GGWCC 1 cut(s) 108
SmiMI CAYNNNNRTG 1 cut(s) 1176
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 4 cut(s) 131, 211, 292, 809
SseBI AGGCCT 2 cut(s) 1076, 1221
SsiI CCGC 4 cut(s) 191, 426, 869, 1034
SspMI CTAG 3 cut(s) 785, 858, 893
SstI GAGCTC 1 cut(s) 836
StuI AGGCCT 2 cut(s) 1076, 1221
StyD4I CCNGG 4 cut(s) 19, 342, 503, 1183
StyI CCWWGG 3 cut(s) 104, 477, 950
TaaI ACNGT 3 cut(s) 289, 384, 517
TaiI ACGT 1 cut(s) 90
TaqI TCGA 1 cut(s) 1136
TasI AATT 4 cut(s) 131, 211, 292, 809
TatI WGTACW 2 cut(s) 175, 564
TauI GCSGC 1 cut(s) 193
TfiI GAWTC 1 cut(s) 1138
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TscAI CASTG 3 cut(s) 627, 642, 735
TseI GCWGC 7 cut(s) 200, 367, 451, 1028, 1052, 1069, 1087
TspDTI ATGAA 3 cut(s) 411, 986, 1069
TspGWI ACGGA 2 cut(s) 16, 20
TspRI CASTG 3 cut(s) 627, 642, 735
Van91I CCANNNNNTGG 1 cut(s) 971
VpaK11BI GGWCC 1 cut(s) 108
XcmI CCANNNNNNNNNTGG 1 cut(s) 550
XmnI GAANNNNTTC 2 cut(s) 66, 1142
XspI CTAG 3 cut(s) 785, 858, 893
Zsp2I ATGCAT 2 cut(s) 6, 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.