Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0005071

Physical Location & Seq

Genomic Coordinates Forward (+)

11000117 .. 11002327

Gene Structure 2211 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2211 bp

ATGGTGTTTTCCTCATCATCGTTACCCATTCTACCCTGCAATTTTCATGTCCTCCCCGCCGCTGACCCACCGTACAAACTCCTCCAAACCCATCCATCTCTCACCCTTCTCTCCAAATGCAAAAGCATCCAAAATCTCAAACAAGTCCACACCCACATCATCAAAACCGGCCTCCACAACACCCACTTTGCCCTCAGCAAGCTCGTCGAGTTTTGCGCCGTTTCGCCCTTCGGGGATCTCTCTTACGCTCTCTCGGTATTCGAATCCATCGAGAACCCTAATCAGATTGTTTGGAACACGATCATTCGAGGTCATTCCTTGAGCTCTAGGCCCATAGAGGCCGTGGAATTCTATGTCCGGATGATCCTTTCGGGAGTGGAGCCGAATTCTTATACTTTCCCTTTTCTTTTGAAGTCTTGTGCAAAAACCGCGGCGACCCACGAAGGGAAACAGGTTCATGGACATGTTGTGAAGCTTGGACTGGAGTCTGATGCCTTTGTGCACACTTCTCTTATCAATATGTATGCACAAAATGGTGAATTGGATAGTGCACGTCTGGTGTTTGATAAAAGTCTTCTGAGAGATGCTGTTTCGTTTACGGCGTTGATCAGCGGTTATGTTTCGAGGGGTTGTATGGATGATGCTCGTGTTCTGTTCGATGAAATACCTGTGAGAGATGTGGTGTCTTGGAATGCCATGATTTCGGGTTATGCTCAAGGGGAGCGATTTGAAGAGGCATTGGCTTTGTTTGGGGAGATGCGGAAAGCAAATGTCTCTCCCAATGAGAGTACTATGGTTACGGTGCTTTCTGCTTGTGCTCAGTCAGGGTCTCTCGAATTGGGCAAATGGGTTGCATCTTGGATTGAGGATCATGGACTTGGTTCAAATCTTCGCCTCATCAGTGCACTTATTGATATGTACTCCAAGTGTGGTGCATTGGACATAGCTCGGGGTTTATTTGATGGTTTGCAGCAAAGGGATGTAATTTCGTGGAATTTAATGATTGGTGGTTATACACATAAGAGCCACTACAAAGAAGCCTTGATGCTGTTTCGGTTGATGCTACGATCAAATACAGAGCCTAATGATGTCACATTCTTAGGCATTCTTCCAGCTTGTGCTCATTTAGGAGCTCTTGATCTTGGCAAGTGGATTCATGCCTACATAGATAAGAACTTCCAGAAGTTTACCAATACTTGCCTCTGGACGAGCCTGATTGACATGTATGCAAAATGCGGAAACATCGAGGCAGCAAAACAGGTCTTTAATGGCATGGAAACCAAAAGCTTGGCTTCTTGGAATGCAATGATATCCGGCTTAGCAATGCACGGGGATGCGCATGCAGCCCTTGAGCTTTTCTCAAAAATGGAGGATGAAGGATTCAACGCAGATGCTATCACATTTGTGGGAGTTTTATCTGCTTGTAACCATGGTGGTCTTGTAGACCTGGGACGCCAATACTTCAGCTCCATGACGACGGATTACCATATTTCACCAGATCTGCATCACTATGGATGCATGATAGATATTCTAGGGCGAGCCGGGTTATTTGATGAAGCTGGGGCCCTAATGAAGAGCATGGAAATGAAGCCAGATGGTGCTGTGTGGGGTTCTCTGCTTGGAGCTTGTAGACTCCACAGGCGCGTGGAGCTGGGTGAATCCGTTGCCAAACATCTGTTCGAATTGGAGCCTGAAAATGCCGGGGCTTATGTTCTGCTATCAAATATCTATGCAGGAGCTGGCAGGTGGGATGATATGGCAAGAATAAGAACGAGGCTGAACGACTTGGGAATCAAGAAAGTTCCTGGCTCTACGTCTATTGAGGTCAATAGTGTTGTTCATGAGTTTCTCGTCAGTGACAAATCTCACCCATTAAGTAAAGAAATCTACGAGATGTTGGAAGAAATCGATAGGCTTTTGGATAAGGCGGGTTTTGTGCCGGATACATCGGAGGTGCTGTACGACATGGGTGAGGAGTGGAAGGAAGTGGCTTTGAGTCATCACAGTGAGAAATTAGCCATTGCTTTCGGTTTGATCAGCACGAAGCCAGGCACAACGATTCGAATAGTAAAGAATCTTCGTGTTTGCGGGAATTGCCATTCAGCAACAAAGCTGATATCCAAGATCTTTAATAGGGAGATTATTGCAAGAGATGGGAACCGTTTCCACCATTTTAGAGATGGTTCTTGTTCATGTAATGACAATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0031425 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1900865 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

737

Amino Acids

81.51

Weight (kDa)

6.13

Isoelectric Point (pI)

39.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	93 - 142	8.9e-09	PPR repeat family
PPR_2	PF13041	225 - 273	1.1e-13	PPR repeat family
PPR	PF01535	228 - 257	6.7e-09	PPR repeat
PPR_2	PF13041	326 - 375	8.2e-11	PPR repeat family
PPR_2	PF13041	431 - 475	7.2e-09	PPR repeat family
PPR	PF01535	432 - 461	2e-07	PPR repeat
E_motif	PF20431	546 - 608	1.3e-19	E motif
Eplus_motif	PF20430	613 - 636	1.4e-09	E+ motif
DYW_deaminase	PF14432	644 - 736	5.3e-39	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0005071)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08070 AT1G08070
fragaria_vesca	FvH4_1g03710 FvH4_1g04070
malus_domestica	MD02G1039500.v1.1 MD15G1176200.v1.1 MD15G1180300.v1.1
prunus_persica	Prupe.7G236200_v2.0.a1 Prupe.7G239300_v2.0.a1
pyrus_communis	pycom02g03280 pycom15g15800 pycom15g16170 pycom15g16180
rosa_chinensis	RchiOBHm_Chr2g0089161 RchiOBHm_Chr2g0089561
rosa_laevigata	RLG00000015999 RLG00000016040
rosa_multiflora	Rmu_co8347093.1_g000001 Rmu_sc0006193.1_g000005 Rmu_sc0008909.1_g000002 Rmu_sc0023591.1_g000001
rosa_rugosa	Rorug01G0488000 Rorug02G0001500
rosa_samantha	Rh2AG043600 Rh2BG042300 Rh2CG043600 Rh2CG047500 Rh2DG042700
rosa_wichuraiana	Rw2G003400 Rw2G003590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1734
Acc16I	TGCGCA	1 cut(s)	1338
Acc36I	ACCTGC	1 cut(s)	1734
AccI	GTMKAC	2 cut(s)	1443, 1630
AccII	CGCG	2 cut(s)	431, 1644
AccIII	TCCGGA	1 cut(s)	357
AciI	CCGC	9 cut(s)	57, 60, 429, 431, 612, 760, 1236, 1928, 2088
AclWI	GGATC	3 cut(s)	243, 358, 876
AcsI	RAATTY	3 cut(s)	347, 385, 994
AcuI	CTGAAG	1 cut(s)	1447
AcyI	GRCGYC	1 cut(s)	1453
AfaI	GTAC	4 cut(s)	74, 790, 920, 1961
AfiI	CCNNNNNNNGG	1 cut(s)	444
AflIII	ACRYGT	2 cut(s)	463, 1221
AgsI	TTSAA	4 cut(s)	412, 731, 885, 1384
AjiI	CACGTC	1 cut(s)	554
AjnI	CCWGG	3 cut(s)	1446, 1804, 2047
AjuI	GAANNNNNNNTTGG	2 cut(s)	1661, 1693
AleI	CACNNNNGTG	1 cut(s)	1403
Alw21I	GWGCWC	7 cut(s)	326, 504, 553, 820, 907, 1123, 1135
Alw26I	GTCTC	2 cut(s)	778, 834
Alw44I	GTGCAC	3 cut(s)	500, 549, 903
AlwI	GGATC	3 cut(s)	243, 358, 876
AlwNI	CAGNNNCTG	1 cut(s)	1739
Ama87I	CYCGRG	1 cut(s)	948
Aor13HI	TCCGGA	1 cut(s)	357
AoxI	GGCC	4 cut(s)	169, 329, 339, 1563
ApaI	GGGCCC	1 cut(s)	1567
ApaLI	GTGCAC	3 cut(s)	500, 549, 903
ApeKI	GCWGC	3 cut(s)	970, 1250, 1343
ApoI	RAATTY	3 cut(s)	347, 385, 994
Asp700I	GAANNNNTTC	1 cut(s)	2162
AspLEI	GCGC	3 cut(s)	218, 1339, 1644
AspS9I	GGNCC	3 cut(s)	330, 1563, 1564
AsuC2I	CCSGG	2 cut(s)	1543, 1702
AsuHPI	GGTGA	6 cut(s)	94, 548, 1485, 1667, 1859, 1982
AsuII	TTCGAA	3 cut(s)	261, 1680, 2062
AvaI	CYCGRG	1 cut(s)	948
BaeGI	GKGCMC	4 cut(s)	504, 553, 907, 1567
BanII	GRGCYC	3 cut(s)	326, 1135, 1567
BarI	GAAGNNNNNNTAC	2 cut(s)	2061, 2093
BauI	CACGAG	1 cut(s)	645
BbsI	GAAGAC	1 cut(s)	566
Bbv12I	GWGCWC	7 cut(s)	326, 504, 553, 820, 907, 1123, 1135
BbvCI	CCTCAGC	1 cut(s)	194
BbvI	GCAGC	3 cut(s)	982, 1262, 1355
BccI	CCATC	7 cut(s)	99, 103, 275, 956, 1589, 2147, 2174
BceAI	ACGGC	3 cut(s)	203, 326, 615
BcgI	CGANNNNNNTGC	2 cut(s)	187, 221
BciT130I	CCWGG	3 cut(s)	1448, 1806, 2049
BciVI	GTATCC	1 cut(s)	1936
BclI	TGATCA	2 cut(s)	606, 2034
BcnI	CCSGG	2 cut(s)	1543, 1702
BcoDI	GTCTC	2 cut(s)	778, 834
BfaI	CTAG	2 cut(s)	327, 1532
BfuAI	ACCTGC	1 cut(s)	1734
BfuI	GTATCC	1 cut(s)	1936
BglII	AGATCT	2 cut(s)	1498, 2124
BisI	GCNGC	5 cut(s)	60, 432, 971, 1251, 1344
BlpI	GCTNAGC	1 cut(s)	1318
BlsI	GCNGC	5 cut(s)	61, 433, 972, 1252, 1345
BmcAI	AGTACT	1 cut(s)	790
Bme1390I	CCNGG	5 cut(s)	1448, 1543, 1702, 1806, 2049
BmeT110I	CYCGRG	1 cut(s)	948
BmgBI	CACGTC	1 cut(s)	554
BmgT120I	GGNCC	3 cut(s)	330, 1563, 1564
BmiI	GGNNCC	5 cut(s)	381, 1564, 1565, 1689, 2159
BmrFI	CCNGG	5 cut(s)	1448, 1543, 1702, 1806, 2049
BoxI	GACNNNNGTC	1 cut(s)	484
BpiI	GAAGAC	1 cut(s)	566
BplI	GAGNNNNNCTC	2 cut(s)	1343, 1375
BpmI	CTGGAG	1 cut(s)	503
Bpu10I	CCTNAGC	1 cut(s)	194
Bpu1102I	GCTNAGC	1 cut(s)	1318
Bpu14I	TTCGAA	3 cut(s)	261, 1680, 2062
BpuEI	CTTGAG	3 cut(s)	340, 699, 1370
BpuMI	CCSGG	2 cut(s)	1543, 1702
Bsa29I	ATCGAT	1 cut(s)	1908
BsaBI	GATNNNNATC	1 cut(s)	1503
BsaHI	GRCGYC	1 cut(s)	1453
BsaI	GGTCTC	1 cut(s)	834
BsaJI	CCNNGG	5 cut(s)	342, 429, 1429, 1447, 1701
BsaWI	WCCGGW	1 cut(s)	357
BsaXI	ACNNNNNCTCC	4 cut(s)	1451, 1481, 1943, 1973
Bsc4I	CCNNNNNNNGG	1 cut(s)	444
Bse118I	RCCGGY	1 cut(s)	167
Bse1I	ACTGG	1 cut(s)	486
Bse3DI	GCAATG	3 cut(s)	1311, 1329, 2019
Bse8I	GATNNNNATC	1 cut(s)	1503
BseAI	TCCGGA	1 cut(s)	357
BseBI	CCWGG	3 cut(s)	1448, 1806, 2049
BseCI	ATCGAT	1 cut(s)	1908
BseDI	CCNNGG	5 cut(s)	342, 429, 1429, 1447, 1701
BseGI	GGATG	9 cut(s)	91, 126, 366, 643, 985, 1339, 1378, 1520, 1756
BseJI	GATNNNNATC	1 cut(s)	1503
BseLI	CCNNNNNNNGG	1 cut(s)	444
BseMI	GCAATG	3 cut(s)	1311, 1329, 2019
BseMII	CTCAG	3 cut(s)	208, 569, 833
BseNI	ACTGG	1 cut(s)	486
BseRI	GAGGAG	2 cut(s)	71, 1988
BseSI	GKGCMC	4 cut(s)	504, 553, 907, 1567
BseXI	GCAGC	3 cut(s)	982, 1262, 1355
BseYI	CCCAGC	2 cut(s)	1559, 1651
Bsh1236I	CGCG	2 cut(s)	431, 1644
BshFI	GGCC	4 cut(s)	171, 331, 341, 1565
BshVI	ATCGAT	1 cut(s)	1908
BsiHKAI	GWGCWC	7 cut(s)	326, 504, 553, 820, 907, 1123, 1135
BsiHKCI	CYCGRG	1 cut(s)	948
BsiSI	CCGG	6 cut(s)	168, 358, 1314, 1542, 1701, 1940
BslFI	GGGAC	1 cut(s)	1464
BslI	CCNNNNNNNGG	1 cut(s)	444
BsmAI	GTCTC	2 cut(s)	778, 834
BsmFI	GGGAC	1 cut(s)	1464
BsmI	GAATGC	3 cut(s)	697, 1104, 1306
BsnI	GGCC	4 cut(s)	171, 331, 341, 1565
Bso31I	GGTCTC	1 cut(s)	834
BsoBI	CYCGRG	1 cut(s)	948
Bsp119I	TTCGAA	3 cut(s)	261, 1680, 2062
Bsp120I	GGGCCC	1 cut(s)	1563
Bsp1286I	GDGCHC	8 cut(s)	326, 504, 553, 820, 907, 1123, 1135, 1567
Bsp13I	TCCGGA	1 cut(s)	357
Bsp1720I	GCTNAGC	1 cut(s)	1318
Bsp19I	CCATGG	1 cut(s)	1429
BspACI	CCGC	9 cut(s)	57, 60, 429, 431, 612, 760, 1236, 1928, 2088
BspANI	GGCC	4 cut(s)	171, 331, 341, 1565
BspCNI	CTCAG	3 cut(s)	207, 570, 832
BspDI	ATCGAT	1 cut(s)	1908
BspEI	TCCGGA	1 cut(s)	357
BspFNI	CGCG	2 cut(s)	431, 1644
BspHI	TCATGA	1 cut(s)	1840
BspLI	GGNNCC	5 cut(s)	381, 1564, 1565, 1689, 2159
BspMI	ACCTGC	1 cut(s)	1734
BspPI	GGATC	3 cut(s)	243, 358, 876
BspQI	GCTCTTC	1 cut(s)	1568
BspT104I	TTCGAA	3 cut(s)	261, 1680, 2062
BspTNI	GGTCTC	1 cut(s)	834
BsrDI	GCAATG	3 cut(s)	1311, 1329, 2019
BsrFI	RCCGGY	1 cut(s)	167
BsrI	ACTGG	1 cut(s)	486
BssAI	RCCGGY	1 cut(s)	167
BssECI	CCNNGG	5 cut(s)	342, 429, 1429, 1447, 1701
BssNI	GRCGYC	1 cut(s)	1453
BssSI	CACGAG	1 cut(s)	645
BssT1I	CCWWGG	1 cut(s)	1429
Bst2BI	CACGAG	1 cut(s)	645
Bst2UI	CCWGG	3 cut(s)	1448, 1806, 2049
Bst4CI	ACNGT	4 cut(s)	72, 802, 2006, 2162
Bst6I	CTCTTC	2 cut(s)	726, 1568
BstACI	GRCGYC	1 cut(s)	1453
BstBI	TTCGAA	3 cut(s)	261, 1680, 2062
BstC8I	GCNNGC	4 cut(s)	200, 1341, 1539, 1741
BstDEI	CTNAG	5 cut(s)	194, 578, 819, 1099, 1318
BstDSI	CCRYGG	3 cut(s)	342, 429, 1429
BstF5I	GGATG	9 cut(s)	91, 126, 366, 643, 985, 1339, 1378, 1520, 1756
BstFNI	CGCG	2 cut(s)	431, 1644
BstHHI	GCGC	3 cut(s)	218, 1339, 1644
BstMAI	GTCTC	2 cut(s)	778, 834
BstMWI	GCNNNNNNNGC	4 cut(s)	428, 1343, 1648, 2094
BstNI	CCWGG	3 cut(s)	1448, 1806, 2049
BstNSI	RCATGY	3 cut(s)	467, 1225, 1343
BstPAI	GACNNNNGTC	1 cut(s)	484
BstSCI	CCNGG	5 cut(s)	1446, 1541, 1700, 1804, 2047
BstSLI	GKGCMC	4 cut(s)	504, 553, 907, 1567
BstUI	CGCG	2 cut(s)	431, 1644
BstV1I	GCAGC	3 cut(s)	982, 1262, 1355
BstV2I	GAAGAC	1 cut(s)	566
BstX2I	RGATCY	3 cut(s)	235, 1498, 2124
BstXI	CCANNNNNNTGG	1 cut(s)	1288
BstYI	RGATCY	3 cut(s)	235, 1498, 2124
Bsu15I	ATCGAT	1 cut(s)	1908
BsuI	GTATCC	1 cut(s)	1936
BsuRI	GGCC	4 cut(s)	171, 331, 341, 1565
BsuTUI	ATCGAT	1 cut(s)	1908
BtgI	CCRYGG	3 cut(s)	342, 429, 1429
BtrI	CACGTC	1 cut(s)	554
BtsCI	GGATG	9 cut(s)	91, 126, 366, 643, 985, 1339, 1378, 1520, 1756
BtsIMutI	CAGTG	3 cut(s)	907, 1861, 2011
BveI	ACCTGC	1 cut(s)	1734
Cac8I	GCNNGC	4 cut(s)	200, 1341, 1539, 1741
CaiI	CAGNNNCTG	1 cut(s)	1739
CciI	TCATGA	1 cut(s)	1840
CfoI	GCGC	3 cut(s)	218, 1339, 1644
Cfr10I	RCCGGY	1 cut(s)	167
Cfr13I	GGNCC	3 cut(s)	330, 1563, 1564
Cfr42I	CCGCGG	1 cut(s)	432
ClaI	ATCGAT	1 cut(s)	1908
CseI	GACGC	1 cut(s)	1461
Csp6I	GTAC	4 cut(s)	73, 789, 919, 1960
CviQI	GTAC	4 cut(s)	73, 789, 919, 1960
DdeI	CTNAG	5 cut(s)	194, 578, 819, 1099, 1318
Eam1104I	CTCTTC	2 cut(s)	726, 1568
EarI	CTCTTC	2 cut(s)	726, 1568
Ecl136II	GAGCTC	2 cut(s)	324, 1133
Eco130I	CCWWGG	1 cut(s)	1429
Eco24I	GRGCYC	3 cut(s)	326, 1135, 1567
Eco31I	GGTCTC	1 cut(s)	834
Eco32I	GATATC	2 cut(s)	1311, 2118
Eco53kI	GAGCTC	2 cut(s)	324, 1133
Eco57I	CTGAAG	1 cut(s)	1447
Eco88I	CYCGRG	1 cut(s)	948
EcoICRI	GAGCTC	2 cut(s)	324, 1133
EcoO109I	RGGNCCY	2 cut(s)	1563, 1564
EcoRI	GAATTC	2 cut(s)	347, 385
EcoRII	CCWGG	3 cut(s)	1446, 1804, 2047
EcoRV	GATATC	2 cut(s)	1311, 2118
EcoT14I	CCWWGG	1 cut(s)	1429
EcoT22I	ATGCAT	1 cut(s)	1520
EcoT38I	GRGCYC	3 cut(s)	326, 1135, 1567
ErhI	CCWWGG	1 cut(s)	1429
FalI	AAGNNNNNCTT	2 cut(s)	1276, 1308
FaqI	GGGAC	1 cut(s)	1464
FauI	CCCGC	3 cut(s)	64, 1921, 2081
FbaI	TGATCA	2 cut(s)	606, 2034
FblI	GTMKAC	2 cut(s)	1443, 1630
Fnu4HI	GCNGC	5 cut(s)	60, 432, 971, 1251, 1344
FokI	GGATG	9 cut(s)	78, 113, 373, 650, 992, 1346, 1385, 1527, 1763
FriOI	GRGCYC	3 cut(s)	326, 1135, 1567
Fsp4HI	GCNGC	5 cut(s)	60, 432, 971, 1251, 1344
FspAI	RTGCGCAY	1 cut(s)	1338
FspBI	CTAG	2 cut(s)	327, 1532
FspI	TGCGCA	1 cut(s)	1338
GlaI	GCGC	3 cut(s)	217, 1338, 1643
GluI	GCNGC	5 cut(s)	60, 432, 971, 1251, 1344
GsaI	CCCAGC	2 cut(s)	1563, 1655
GsuI	CTGGAG	1 cut(s)	503
HaeIII	GGCC	4 cut(s)	171, 331, 341, 1565
HapII	CCGG	6 cut(s)	168, 358, 1314, 1542, 1701, 1940
HgaI	GACGC	1 cut(s)	1461
HhaI	GCGC	3 cut(s)	218, 1339, 1644
Hin1I	GRCGYC	1 cut(s)	1453
Hin6I	GCGC	3 cut(s)	216, 1337, 1642
HinP1I	GCGC	3 cut(s)	216, 1337, 1642
HindIII	AAGCTT	2 cut(s)	473, 1285
HpaII	CCGG	6 cut(s)	168, 358, 1314, 1542, 1701, 1940
HphI	GGTGA	6 cut(s)	94, 548, 1485, 1667, 1859, 1982
Hpy166II	GTNNAC	8 cut(s)	148, 502, 551, 597, 905, 1188, 1444, 1631
Hpy188I	TCNGA	4 cut(s)	285, 490, 579, 1951
Hpy188III	TCNNGA	9 cut(s)	271, 358, 372, 833, 1136, 1180, 1204, 1795, 1841
Hpy8I	GTNNAC	8 cut(s)	148, 502, 551, 597, 905, 1188, 1444, 1631
Hpy99I	CGWCG	2 cut(s)	209, 1480
HpyAV	CCTTC	5 cut(s)	116, 238, 437, 1370, 1975
HpyCH4III	ACNGT	4 cut(s)	72, 802, 2006, 2162
HpyCH4IV	ACGT	2 cut(s)	553, 1814
HpyF10VI	GCNNNNNNNGC	4 cut(s)	428, 1343, 1648, 2094
HpyF3I	CTNAG	5 cut(s)	194, 578, 819, 1099, 1318
HpySE526I	ACGT	2 cut(s)	553, 1814
Hsp92I	GRCGYC	1 cut(s)	1453
HspAI	GCGC	3 cut(s)	216, 1337, 1642
Kpn2I	TCCGGA	1 cut(s)	357
Ksp22I	TGATCA	2 cut(s)	606, 2034
KspI	CCGCGG	1 cut(s)	432
LguI	GCTCTTC	1 cut(s)	1568
LmnI	GCTCC	8 cut(s)	379, 721, 1130, 1472, 1622, 1648, 1687, 1736
Lsp1109I	GCAGC	3 cut(s)	982, 1262, 1355
MaeI	CTAG	2 cut(s)	327, 1532
MaeII	ACGT	2 cut(s)	553, 1814
MaeIII	GTNAC	5 cut(s)	21, 796, 1090, 1424, 1856
MboII	GAAGA	7 cut(s)	566, 743, 881, 1100, 1585, 1913, 2069
MfeI	CAATTG	1 cut(s)	2203
MflI	RGATCY	3 cut(s)	235, 1498, 2124
MhlI	GDGCHC	8 cut(s)	326, 504, 553, 820, 907, 1123, 1135, 1567
MlyI	GAGTC	3 cut(s)	494, 1626, 2005
MmeI	TCCRAC	1 cut(s)	1878
Mph1103I	ATGCAT	1 cut(s)	1520
MroI	TCCGGA	1 cut(s)	357
MroXI	GAANNNNTTC	1 cut(s)	2162
MseI	TTAA	4 cut(s)	998, 1266, 1874, 2130
MslI	CAYNNNNRTG	6 cut(s)	462, 1332, 1403, 1509, 1583, 2004
MspA1I	CMGCKG	3 cut(s)	62, 431, 612
MspI	CCGG	6 cut(s)	168, 358, 1314, 1542, 1701, 1940
MspR9I	CCNGG	5 cut(s)	1448, 1543, 1702, 1806, 2049
MunI	CAATTG	1 cut(s)	2203
Mva1269I	GAATGC	3 cut(s)	697, 1104, 1306
MvaI	CCWGG	3 cut(s)	1448, 1806, 2049
MvnI	CGCG	2 cut(s)	431, 1644
MwoI	GCNNNNNNNGC	4 cut(s)	428, 1343, 1648, 2094
NciI	CCSGG	2 cut(s)	1543, 1702
NcoI	CCATGG	1 cut(s)	1429
NlaIV	GGNNCC	5 cut(s)	381, 1564, 1565, 1689, 2159
NmuCI	GTSAC	2 cut(s)	1090, 1856
NsbI	TGCGCA	1 cut(s)	1338
NsiI	ATGCAT	1 cut(s)	1520
NspI	RCATGY	3 cut(s)	467, 1225, 1343
NspV	TTCGAA	3 cut(s)	261, 1680, 2062
OliI	CACNNNNGTG	1 cut(s)	1403
PaeI	GCATGC	1 cut(s)	1343
PagI	TCATGA	1 cut(s)	1840
PaqCI	CACCTGC	1 cut(s)	1734
PciI	ACATGT	2 cut(s)	463, 1221
PciSI	GCTCTTC	1 cut(s)	1568
PcsI	WCGNNNNNNNCGW	2 cut(s)	267, 599
PctI	GAATGC	3 cut(s)	697, 1104, 1306
PdmI	GAANNNNTTC	1 cut(s)	2162
PfeI	GAWTC	7 cut(s)	263, 1153, 1380, 1658, 1791, 2059, 2074
PkrI	GCNGC	5 cut(s)	61, 433, 972, 1252, 1345
PleI	GAGTC	3 cut(s)	493, 1626, 2004
PpsI	GAGTC	3 cut(s)	493, 1626, 2004
PscI	ACATGT	2 cut(s)	463, 1221
PshAI	GACNNNNGTC	1 cut(s)	484
Psp124BI	GAGCTC	2 cut(s)	326, 1135
Psp6I	CCWGG	3 cut(s)	1446, 1804, 2047
PspFI	CCCAGC	2 cut(s)	1559, 1651
PspGI	CCWGG	3 cut(s)	1446, 1804, 2047
PspN4I	GGNNCC	5 cut(s)	381, 1564, 1565, 1689, 2159
PspOMI	GGGCCC	1 cut(s)	1563
PspPI	GGNCC	3 cut(s)	330, 1563, 1564
PstNI	CAGNNNCTG	1 cut(s)	1739
PsuI	RGATCY	3 cut(s)	235, 1498, 2124
RsaI	GTAC	4 cut(s)	74, 790, 920, 1961
RsaNI	GTAC	4 cut(s)	73, 789, 919, 1960
RseI	CAYNNNNRTG	6 cut(s)	462, 1332, 1403, 1509, 1583, 2004
SacI	GAGCTC	2 cut(s)	326, 1135
SacII	CCGCGG	1 cut(s)	432
SapI	GCTCTTC	1 cut(s)	1568
SaqAI	TTAA	4 cut(s)	998, 1266, 1874, 2130
SatI	GCNGC	5 cut(s)	60, 432, 971, 1251, 1344
Sau96I	GGNCC	3 cut(s)	330, 1563, 1564
ScaI	AGTACT	1 cut(s)	790
SchI	GAGTC	3 cut(s)	494, 1626, 2005
ScrFI	CCNGG	5 cut(s)	1448, 1543, 1702, 1806, 2049
SduI	GDGCHC	8 cut(s)	326, 504, 553, 820, 907, 1123, 1135, 1567
Sfr303I	CCGCGG	1 cut(s)	432
SfuI	TTCGAA	3 cut(s)	261, 1680, 2062
SgrBI	CCGCGG	1 cut(s)	432
SmiMI	CAYNNNNRTG	6 cut(s)	462, 1332, 1403, 1509, 1583, 2004
SmlI	CTYRAG	3 cut(s)	319, 714, 1349
SmoI	CTYRAG	3 cut(s)	319, 714, 1349
SphI	GCATGC	1 cut(s)	1343
SsiI	CCGC	9 cut(s)	57, 60, 429, 431, 612, 760, 1236, 1928, 2088
SspMI	CTAG	2 cut(s)	327, 1532
SstI	GAGCTC	2 cut(s)	326, 1135
StyD4I	CCNGG	5 cut(s)	1446, 1541, 1700, 1804, 2047
StyI	CCWWGG	1 cut(s)	1429
TaaI	ACNGT	4 cut(s)	72, 802, 2006, 2162
TaiI	ACGT	2 cut(s)	556, 1817
TatI	WGTACW	2 cut(s)	788, 918
TauI	GCSGC	2 cut(s)	62, 434
TfiI	GAWTC	7 cut(s)	263, 1153, 1380, 1658, 1791, 2059, 2074
Tru1I	TTAA	4 cut(s)	998, 1266, 1874, 2130
Tru9I	TTAA	4 cut(s)	998, 1266, 1874, 2130
TscAI	CASTG	3 cut(s)	907, 1861, 2011
TseFI	GTSAC	2 cut(s)	1090, 1856
TseI	GCWGC	3 cut(s)	970, 1250, 1343
Tsp45I	GTSAC	2 cut(s)	1090, 1856
TspGWI	ACGGA	2 cut(s)	1493, 1651
TspRI	CASTG	3 cut(s)	907, 1861, 2011
VneI	GTGCAC	3 cut(s)	500, 549, 903
XapI	RAATTY	3 cut(s)	347, 385, 994
XceI	RCATGY	3 cut(s)	467, 1225, 1343
XcmI	CCANNNNNNNNNTGG	2 cut(s)	340, 2177
XmiI	GTMKAC	2 cut(s)	1443, 1630
XmnI	GAANNNNTTC	1 cut(s)	2162
XspI	CTAG	2 cut(s)	327, 1532
ZrmI	AGTACT	1 cut(s)	790
Zsp2I	ATGCAT	1 cut(s)	1520

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.