Rw2G003590

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005071
Physical Location & Seq
Forward (+)
3305342 .. 3312644
7303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003590.1

Sequence Viewer

Length: 3987 bp
ATGGCTGCTCCGCTTTCCCTCCACCACATTAGACCACATTTCTTTCCCGACAATCCCCTCTTAAATAAACCCACCGCCGTAACGGTCGCAACTACCACCGCCGCGGCGGCTACCAAAACTAACCAAGCTCAACAACACCCATGTCTCACGTTACTAGACAACTGCTCCAGCATGTCTGAGCTCAAGCAAATCCACGCCCAGCTCCTCCGGAACGGTCTCTTCTTCGACGCATTCACCGCCAGCAAGATCGTCGCCTTTTCGGCTTTAGAAGACTCCGGAAACCCCACTTATTCCCGTTTAGTCCTCACCCAAATCCCCAATCCCAATGGTTTCACTTGTAATTCTGTTATCCGAGGCTATACGAACAAGGGTTTGGCCCGGGAGGCCATTTCTTTCTACCGAGAAATGATTGCACGGGGTTGGATACCTGACCGGTTCACATTCCCTTCGTTGTTCAAGTCATGTAAAGATTTGGGTGAAGGGAAACAGGTGCATTGCCAATCTACCAAGCTAGGCTTCGCTTCGGATTCGTACATTCAGAACACTCTGATGAACATGTACTCGACTTGCGGGTGTTTGGTCTCTGCAAGGAAGGTGTTTGATAAAATGAGGGACAAGAGTGTGGTGTCTTGGGCGACCATGATCGACGCTTATGCACAGTGGGATCAATCGGCTGAAGCTTTGAAGCTGTTTTATAAGATGGAGAGGGAAAATGTGGATCCTAATGAGGTTACTTTGCTTAATGTTTTGACTGCGTGTGGGATGGCGAGGGATTTGGAAACTGCGAAAAGGGTGCACCGGTGCATTGAGGAGTACAGTTTTGGGTCGCATTTGAAGCTCAGTACGGCTCTTATGGATGTTTATTGCAAATGTGGATGTGTATCGCTTGCGCGAGAATTGTTCGACAGAATGCCTGAGAAGAACTTGTTCTGTTGGAACATCATGATCAATGGACATGTAGAGGAAAGTGATTATGATGAGGCGTTCCTACTCTTTCGTAGAATGCAACTTGAGGGAGAAAAGGGGGATGAGGTGACTATGGTGAGTTTATTGCTTGCTTGCAGCAATTTGGGAGCTCTAGAGCTTGGAAAGTGGCTGCACGCCTATATTGAAAAGCAGAAAATTGAGGTGGACGTTGCCCTCGGGACGGCTCTTGTTAACATGTACGCCAAGTGCGGGAGCATAGACATTGCATCGGATGTTTTCCGAACACTGCCTGAGAAGGATTTGATGACTTGGACAGCCTTGATTTCTGGTTTTGCAATGTGTGGCCAGGGGAAGAAGGCTTTGGAGCATTTCCATGAGATGCAGATGAGTGGAGTGAAACCAGATGCAATAACCTTTGTTGGGGTATTGGCTGCTTGTAGCCATGCTGGGTTTGTGGATGAAGGACTTTCACATTTTAACTCAATGTCTGAAGTGTATGGGATTCAGCCTAGCATTGAACACTATGGCTGTATGGTTGATGTGTTAGGCAGAGCTGGTCGCATAGCTGAAGCAGAGGAGTTGATTCAAAAGATGCGAATGAAACCAGACCGTTTCGTTTTGGGAGGGCTTCTTGGTGCCTGCAGAATCCATGGCAACCTTGAGGCTGCAGAAAGAGCAGCTAAACAGCTCCTTGAACTTAATCCGGATGATGAAGGAACATATGTGCTTCTATCAAACTTATACAGCTCTATGGAAAAATGGGAAGAAGCGGTAAGAATTAGGAAACTCATGGCAGAAAGGAATATTAAGAAACCTCCTGGATGCAGTCTGATTGAGGTCGATGGAATTGTCCATGAATTTGTGAAGGGTGATTCATCTCACCCACAAACCACAAACATCTACGAAATGCTTGAAGACATGATCAGCCGATTGAAGAAAGCCGGTTATGTCCCTCAGAAATCAGAGGTGTTGTTTGATATGGATGAAGAAGAGAAGGAGACTGCACTTAGCCTCCACAGCGAAAAACTAGCAATTGCATTTGGGCTCATTAGTACAAATCCTGGGACGCCGATACGAGTAGTGAAAAACCTTCGTGTATGCAGTGATTGCCACACTGCAACCAAGCTCATATCCAAAGTTTACAACAGGGAGATAATAGTTCGGGACAGGAATCGTTTCCATCAATTTAAAGATGGATCTTGTTCTTCATCATGCATCCAACTCCTCAAGAGCTGCAAATCCATGAAGCAGCTCAAACAGATCCAAACCCAGATGTTCCTTCTTGGACTACACCAATGCAGAGCCACCCTCAACAGGCTCATGGTCTTCTGCACAGACCCAACTCTTGGAGACTTGCATTACGCCGAGAAAATCTTCGATCACATCCAAACCCCTGGTTTGTTCATCTACAATGTAATGATCAAAGCTTTAGCCAAAAGGAGAAGATTCAAAAGCGTCATTGAGCTCTATCGGCGGTTGAGGGAAGAGGGCTTGTGGCCCGATAATTTCACGTACCCATTTGCTTGTAAAGCCATTGGAGGCTTGGGAGAGGCTCAGGAAGGTGCAAAGGTTCATGGGTTTGCGGTAAAGACTGGATTTCAGTTCGATTCGTATGTGTGTAACTCACTCATTGACATGTATGCGGAATTGGGTCTAGTTGATAATGTGAAGCAGGTGTTTGATGAGATGCCTGAGAGAGATGTTGTTTCTTGGAATGTTACGATTTCAGGGTGTGTTAGGTGTATGAGGTTTAGGGAGGCAGTGAGTGTGTTCCGGCGGATGAGAAAAGAGAGCAATGAGAAGCCGGATGAAGCTACAGTTGTGAGCACGCTTTCGGCTTGCGCAGCACTGAAAGATGTGGAGCTTGGTAGAGAAATTCATGACTATGTTAGAGCTGAGCTTAATTTCACTAGTATAATCGGAAATGCGGTGTTGGATATGTATGCTAAGTGTGGTTGTTTGAGTGAAGCAAGGAAAGTTTTTGATGAAATTCAGTTGAAAAATGTCATGTGTTGGACTAGTATGGTGTGTGGGTATGTGAATTGTGGTATGCTGGATGAGGCTAGAGAGTTGTTTGATAGGAGTCCTACTAAGGATGTTGTTCTATGGACGGCTATGATGAATGGGTATGTACAATATAACCAATTTGATGAGGCAGTGGCTCTATTCCAAGAGATGCAGTTTAGAAGGGTTCAAGTGGATAAATTCACAGTCGTTGCGCTCCTCACAGGCTGTGCTCAGTTGGGAGCATTAGAGCAAGGGAAATGGATTCACGGATACATAGACGAAAATGGAATCAAAATTGATGCAGTTGTTGGTACTGCTCTTATAGAAATGTATGCAAAATGTGGGTGCATAGACAAGTCTCTGGAGATTTTCAATGCATTGAATGAAAAAGATGCAGCCACTTGGACTTCAATGGTCTGCGCGCTTGCAATGAATGGAAAGACAAGCAAAGCACTCGAGTGGTTCTCCAAAATGAAGCAGGTTAAGATCAACCCTGATGATATCACCTTCATTGGTGTCTTGAGTGCTTGTAGTCATGGAGGATTGGTTGACGAAGGACGAAAGTTGTTCAACTCCATGAGAAAGGACTATGGGATCGAGCCAAAGCTAGAACATTACGGGTGTCTGATTGACCTTCTTGGTCGGGCTGGACTCCTAGACGAGGCAGAGGAGCTGATAGAAAGTATTCCCAGTGAAAACAATAAGATTGTAGTTCCACTTTACAGTTCTTTACTCAGTGCTTGCAGAATCCATGGCAATGTTAACATGAGTGAAAGAGTGGCTAAAAGGCTATTAGATATTGAGTCTAGCTGCTCGAGTGCTCACACGCTTCTGGCCAACATCTACGCAGATGCTGAGAGATGGGAAGATAAGCCTGGGTGCAGTTCGGTTGAGATTAATGGCATTGTCCATGAGTTCCTTGTTGGTGATTCATCTCACCCAGAAACGGAAAAGATATACTCTATGTTGCATAGATTGATAAAGCCATTAGTGGATTTAGAAGAGAATTGCGGAATAGAAACAGAAATCTCAGGTTTAGTAAGTTTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

1328

Amino Acids

148.82

Weight (kDa)

5.93

Isoelectric Point (pI)

36.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 108 - 155 2.3e-09 PPR repeat family
PPR_2 PF13041 207 - 253 4.9e-08 PPR repeat family
PPR_2 PF13041 308 - 355 1.1e-07 PPR repeat family
PPR_2 PF13041 409 - 456 1.2e-09 PPR repeat family
PPR PF01535 412 - 441 4.2e-06 PPR repeat
E_motif PF20431 526 - 588 5e-21 E motif
DYW_deaminase PF14432 624 - 712 2e-36 DYW family of nucleic acid deaminases
PPR_2 PF13041 774 - 814 2.3e-06 PPR repeat family
PPR_2 PF13041 875 - 924 3.7e-07 PPR repeat family
PPR_2 PF13041 1007 - 1055 8.4e-12 PPR repeat family
PPR PF01535 1010 - 1038 4.7e-06 PPR repeat
PPR_2 PF13041 1109 - 1156 3.2e-08 PPR repeat family
E_motif PF20431 1229 - 1268 3.6e-06 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 696
AarI CACCTGC 1 cut(s) 2590
Acc16I TGCGCA 1 cut(s) 2770
Acc36I ACCTGC 2 cut(s) 2590, 3403
AccB1I GGYRCC 1 cut(s) 1562
AccB7I CCANNNNNTGG 1 cut(s) 2273
AccII CGCG 3 cut(s) 104, 892, 3356
AccIII TCCGGA 3 cut(s) 207, 275, 1630
AclWI GGATC 6 cut(s) 672, 713, 726, 2131, 2182, 3536
AcoI YGGCCR 2 cut(s) 1270, 3768
AcsI RAATTY 4 cut(s) 1784, 2802, 2916, 3131
AcuI CTGAAG 3 cut(s) 696, 1437, 1515
AcyI GRCGYC 1 cut(s) 1994
AfaI GTAC 9 cut(s) 533, 560, 815, 844, 1166, 1981, 2441, 3060, 3247
AfiI CCNNNNNNNGG 7 cut(s) 1147, 1176, 1347, 2241, 2273, 3595, 3880
AflIII ACRYGT 4 cut(s) 555, 955, 1161, 2562
AgeI ACCGGT 2 cut(s) 432, 798
AhlI ACTAGT 2 cut(s) 2837, 2945
AjnI CCWGG 5 cut(s) 1272, 1744, 1987, 2320, 3808
AjuI GAANNNNNNNTTGG 6 cut(s) 356, 388, 500, 532, 1271, 1303
AleI CACNNNNGTG 1 cut(s) 3391
Alw21I GWGCWC 7 cut(s) 183, 798, 1078, 2394, 2756, 3166, 3757
Alw26I GTCTC 6 cut(s) 149, 221, 586, 1919, 2271, 3297
Alw44I GTGCAC 1 cut(s) 794
AlwI GGATC 6 cut(s) 672, 713, 726, 2131, 2182, 3536
AlwNI CAGNNNCTG 1 cut(s) 3788
Ama87I CYCGRG 4 cut(s) 378, 1142, 3389, 3748
Aor13HI TCCGGA 3 cut(s) 207, 275, 1630
AoxI GGCC 5 cut(s) 375, 384, 1270, 2423, 3768
ApaLI GTGCAC 1 cut(s) 794
ApoI RAATTY 4 cut(s) 1784, 2802, 2916, 3131
AseI ATTAAT 1 cut(s) 3831
AsiGI ACCGGT 2 cut(s) 432, 798
Asp700I GAANNNNTTC 4 cut(s) 926, 2102, 2300, 3618
AspLEI GCGC 5 cut(s) 892, 2771, 3148, 3356, 3358
AspS9I GGNCC 2 cut(s) 376, 2424
AsuC2I CCSGG 2 cut(s) 379, 380
AvaI CYCGRG 4 cut(s) 378, 1142, 3389, 3748
BaeGI GKGCMC 1 cut(s) 798
BalI TGGCCA 2 cut(s) 1272, 3770
BamHI GGATCC 1 cut(s) 718
BanI GGYRCC 1 cut(s) 1562
BanII GRGCYC 4 cut(s) 183, 1078, 1974, 2394
BbsI GAAGAC 3 cut(s) 276, 1848, 2245
Bbv12I GWGCWC 7 cut(s) 183, 798, 1078, 2394, 2756, 3166, 3757
BccI CCATC 6 cut(s) 694, 757, 1763, 2114, 2115, 3789
BceAI ACGGC 4 cut(s) 62, 861, 1164, 3054
BcgI CGANNNNNNTGC 8 cut(s) 232, 266, 635, 669, 775, 809, 3370, 3404
BciT130I CCWGG 5 cut(s) 1274, 1746, 1989, 2322, 3810
BciVI GTATCC 2 cut(s) 417, 3197
BclI TGATCA 3 cut(s) 945, 1848, 2346
BcnI CCSGG 2 cut(s) 379, 380
BcoDI GTCTC 6 cut(s) 149, 221, 586, 1919, 2271, 3297
BcuI ACTAGT 2 cut(s) 2837, 2945
BfmI CTRYAG 3 cut(s) 1567, 1593, 2742
BfuAI ACCTGC 2 cut(s) 2590, 3403
BfuI GTATCC 2 cut(s) 417, 3197
BglI GCCNNNNNGGC 3 cut(s) 107, 260, 383
BlpI GCTNAGC 1 cut(s) 2823
Bme1390I CCNGG 7 cut(s) 379, 380, 1274, 1746, 1989, 2322, 3810
BmeT110I CYCGRG 4 cut(s) 378, 1142, 3389, 3748
BmgT120I GGNCC 2 cut(s) 376, 2424
BmiI GGNNCC 2 cut(s) 720, 1564
BmrFI CCNGG 7 cut(s) 379, 380, 1274, 1746, 1989, 2322, 3810
BmrI ACTGGG 1 cut(s) 3618
BmuI ACTGGG 1 cut(s) 3618
BpiI GAAGAC 3 cut(s) 276, 1848, 2245
BplI GAGNNNNNCTC 4 cut(s) 2220, 2252, 3383, 3415
BpmI CTGGAG 2 cut(s) 151, 3317
Bpu10I CCTNAGC 1 cut(s) 2481
Bpu1102I GCTNAGC 1 cut(s) 2823
BpuEI CTTGAG 5 cut(s) 167, 1031, 1607, 2138, 3475
BpuMI CCSGG 2 cut(s) 379, 380
BsaAI YACGTR 1 cut(s) 2439
BsaBI GATNNNNATC 1 cut(s) 880
BsaHI GRCGYC 1 cut(s) 1994
BsaI GGTCTC 2 cut(s) 221, 586
BsaWI WCCGGW 5 cut(s) 207, 275, 432, 798, 1630
BsaXI ACNNNNNCTCC 6 cut(s) 149, 179, 1923, 1953, 3465, 3495
Bsc4I CCNNNNNNNGG 7 cut(s) 1147, 1176, 1347, 2241, 2273, 3595, 3880
Bse118I RCCGGY 3 cut(s) 432, 798, 1868
Bse1I ACTGG 2 cut(s) 2524, 3624
Bse3DI GCAATG 6 cut(s) 493, 1188, 1269, 2728, 3369, 3697
Bse8I GATNNNNATC 1 cut(s) 880
BseAI TCCGGA 3 cut(s) 207, 275, 1630
BseBI CCWGG 5 cut(s) 1274, 1746, 1989, 2322, 3810
BseJI GATNNNNATC 1 cut(s) 880
BseLI CCNNNNNNNGG 7 cut(s) 1147, 1176, 1347, 2241, 2273, 3595, 3880
BseMI GCAATG 6 cut(s) 493, 1188, 1269, 2728, 3369, 3697
BseNI ACTGG 2 cut(s) 2524, 3624
BsePI GCGCGC 1 cut(s) 3354
BseRI GAGGAG 6 cut(s) 194, 824, 1517, 2141, 3140, 3617
BseSI GKGCMC 1 cut(s) 798
BseYI CCCAGC 2 cut(s) 198, 1373
BsgI GTGCAG 4 cut(s) 1082, 1914, 2242, 3835
Bsh1236I CGCG 3 cut(s) 104, 892, 3356
Bsh1285I CGRYCG 1 cut(s) 87
BshFI GGCC 5 cut(s) 377, 386, 1272, 2425, 3770
BshNI GGYRCC 1 cut(s) 1562
BshTI ACCGGT 2 cut(s) 432, 798
BsiEI CGRYCG 1 cut(s) 87
BsiHKAI GWGCWC 7 cut(s) 183, 798, 1078, 2394, 2756, 3166, 3757
BsiHKCI CYCGRG 4 cut(s) 378, 1142, 3389, 3748
BsiSI CCGG 9 cut(s) 208, 276, 379, 433, 799, 1631, 1869, 2701, 2732
BslFI GGGAC 5 cut(s) 626, 1159, 1862, 2005, 2105
BslI CCNNNNNNNGG 7 cut(s) 1147, 1176, 1347, 2241, 2273, 3595, 3880
BsmAI GTCTC 6 cut(s) 149, 221, 586, 1919, 2271, 3297
BsmFI GGGAC 5 cut(s) 626, 1159, 1862, 2005, 2105
BsmI GAATGC 3 cut(s) 230, 915, 1008
BsnI GGCC 5 cut(s) 377, 386, 1272, 2425, 3770
Bso31I GGTCTC 2 cut(s) 221, 586
BsoBI CYCGRG 4 cut(s) 378, 1142, 3389, 3748
Bsp1286I GDGCHC 8 cut(s) 183, 798, 1078, 1974, 2394, 2756, 3166, 3757
Bsp13I TCCGGA 3 cut(s) 207, 275, 1630
Bsp1407I TGTACA 1 cut(s) 3058
Bsp1720I GCTNAGC 1 cut(s) 2823
Bsp19I CCATGG 2 cut(s) 1576, 3685
BspANI GGCC 5 cut(s) 377, 386, 1272, 2425, 3770
BspEI TCCGGA 3 cut(s) 207, 275, 1630
BspFNI CGCG 3 cut(s) 104, 892, 3356
BspHI TCATGA 2 cut(s) 942, 2806
BspLI GGNNCC 2 cut(s) 720, 1564
BspMAI CTGCAG 2 cut(s) 1571, 1597
BspMI ACCTGC 2 cut(s) 2590, 3403
BspPI GGATC 6 cut(s) 672, 713, 726, 2131, 2182, 3536
BspT107I GGYRCC 1 cut(s) 1562
BspTNI GGTCTC 2 cut(s) 221, 586
BsrDI GCAATG 6 cut(s) 493, 1188, 1269, 2728, 3369, 3697
BsrFI RCCGGY 3 cut(s) 432, 798, 1868
BsrGI TGTACA 1 cut(s) 3058
BsrI ACTGG 2 cut(s) 2524, 3624
BssAI RCCGGY 3 cut(s) 432, 798, 1868
BssHII GCGCGC 1 cut(s) 3354
BssNI GRCGYC 1 cut(s) 1994
BssT1I CCWWGG 2 cut(s) 1576, 3685
Bst2UI CCWGG 5 cut(s) 1274, 1746, 1989, 2322, 3810
Bst4CI ACNGT 8 cut(s) 85, 215, 660, 818, 1538, 2746, 3139, 3659
Bst6I CTCTTC 4 cut(s) 224, 1911, 2406, 3930
BstACI GRCGYC 1 cut(s) 1994
BstAPI GCANNNNNTGC 1 cut(s) 2034
BstAUI TGTACA 1 cut(s) 3058
BstBAI YACGTR 1 cut(s) 2439
BstDSI CCRYGG 3 cut(s) 102, 1576, 3685
BstENI CCTNNNNNAGG 1 cut(s) 3593
BstFNI CGCG 3 cut(s) 104, 892, 3356
BstHHI GCGC 5 cut(s) 892, 2771, 3148, 3356, 3358
BstMAI GTCTC 6 cut(s) 149, 221, 586, 1919, 2271, 3297
BstMCI CGRYCG 1 cut(s) 87
BstNI CCWGG 5 cut(s) 1274, 1746, 1989, 2322, 3810
BstNSI RCATGY 5 cut(s) 175, 559, 959, 1165, 2566
BstSCI CCNGG 7 cut(s) 377, 378, 1272, 1744, 1987, 2320, 3808
BstSFI CTRYAG 3 cut(s) 1567, 1593, 2742
BstSLI GKGCMC 1 cut(s) 798
BstUI CGCG 3 cut(s) 104, 892, 3356
BstV2I GAAGAC 3 cut(s) 276, 1848, 2245
BstX2I RGATCY 3 cut(s) 718, 2123, 2187
BstXI CCANNNNNNTGG 1 cut(s) 2321
BstYI RGATCY 3 cut(s) 718, 2123, 2187
BsuI GTATCC 2 cut(s) 417, 3197
BsuRI GGCC 5 cut(s) 377, 386, 1272, 2425, 3770
BtgI CCRYGG 3 cut(s) 102, 1576, 3685
BtsI GCAGTG 5 cut(s) 1211, 2035, 2040, 2694, 3090
BtsIMutI CAGTG 9 cut(s) 665, 1211, 2035, 2040, 2694, 2774, 3090, 3631, 3676
BveI ACCTGC 2 cut(s) 2590, 3403
CaiI CAGNNNCTG 1 cut(s) 3788
CciI TCATGA 2 cut(s) 942, 2806
CfoI GCGC 5 cut(s) 892, 2771, 3148, 3356, 3358
Cfr10I RCCGGY 3 cut(s) 432, 798, 1868
Cfr13I GGNCC 2 cut(s) 376, 2424
Cfr42I CCGCGG 1 cut(s) 105
Cfr9I CCCGGG 1 cut(s) 378
CseI GACGC 4 cut(s) 236, 656, 2002, 2371
Csp6I GTAC 9 cut(s) 532, 559, 814, 843, 1165, 1980, 2440, 3059, 3246
CspAI ACCGGT 2 cut(s) 432, 798
CviQI GTAC 9 cut(s) 532, 559, 814, 843, 1165, 1980, 2440, 3059, 3246
DraI TTTAAA 1 cut(s) 2116
EaeI YGGCCR 2 cut(s) 1270, 3768
Eam1104I CTCTTC 4 cut(s) 224, 1911, 2406, 3930
EarI CTCTTC 4 cut(s) 224, 1911, 2406, 3930
EciI GGCGGA 1 cut(s) 2719
Ecl136II GAGCTC 3 cut(s) 181, 1076, 2392
Eco130I CCWWGG 2 cut(s) 1576, 3685
Eco24I GRGCYC 4 cut(s) 183, 1078, 1974, 2394
Eco31I GGTCTC 2 cut(s) 221, 586
Eco32I GATATC 1 cut(s) 3436
Eco53kI GAGCTC 3 cut(s) 181, 1076, 2392
Eco57I CTGAAG 3 cut(s) 696, 1437, 1515
Eco88I CYCGRG 4 cut(s) 378, 1142, 3389, 3748
EcoICRI GAGCTC 3 cut(s) 181, 1076, 2392
EcoNI CCTNNNNNAGG 1 cut(s) 3593
EcoRII CCWGG 5 cut(s) 1272, 1744, 1987, 2320, 3808
EcoRV GATATC 1 cut(s) 3436
EcoT14I CCWWGG 2 cut(s) 1576, 3685
EcoT22I ATGCAT 2 cut(s) 2144, 3313
EcoT38I GRGCYC 4 cut(s) 183, 1078, 1974, 2394
ErhI CCWWGG 2 cut(s) 1576, 3685
FalI AAGNNNNNCTT 2 cut(s) 500, 532
FaqI GGGAC 5 cut(s) 626, 1159, 1862, 2005, 2105
FauI CCCGC 2 cut(s) 563, 1169
FauNDI CATATG 1 cut(s) 1648
FbaI TGATCA 3 cut(s) 945, 1848, 2346
FriOI GRGCYC 4 cut(s) 183, 1078, 1974, 2394
FspI TGCGCA 1 cut(s) 2770
GlaI GCGC 5 cut(s) 891, 2770, 3147, 3355, 3357
GsaI CCCAGC 2 cut(s) 202, 1377
GsuI CTGGAG 2 cut(s) 151, 3317
HaeIII GGCC 5 cut(s) 377, 386, 1272, 2425, 3770
HapII CCGG 9 cut(s) 208, 276, 379, 433, 799, 1631, 1869, 2701, 2732
HgaI GACGC 4 cut(s) 236, 656, 2002, 2371
HhaI GCGC 5 cut(s) 892, 2771, 3148, 3356, 3358
Hin1I GRCGYC 1 cut(s) 1994
Hin6I GCGC 5 cut(s) 890, 2769, 3146, 3354, 3356
HinP1I GCGC 5 cut(s) 890, 2769, 3146, 3354, 3356
HincII GTYRAC 3 cut(s) 1159, 3484, 3697
HindII GTYRAC 3 cut(s) 1159, 3484, 3697
HindIII AAGCTT 2 cut(s) 678, 2352
HpaI GTTAAC 2 cut(s) 1159, 3697
HpaII CCGG 9 cut(s) 208, 276, 379, 433, 799, 1631, 1869, 2701, 2732
Hpy166II GTNNAC 7 cut(s) 438, 796, 1132, 1159, 2068, 3484, 3697
Hpy8I GTNNAC 7 cut(s) 438, 796, 1132, 1159, 2068, 3484, 3697
Hpy99I CGWCG 3 cut(s) 230, 254, 650
HpyCH4III ACNGT 8 cut(s) 85, 215, 660, 818, 1538, 2746, 3139, 3659
HpyCH4IV ACGT 3 cut(s) 149, 1134, 2438
HpySE526I ACGT 3 cut(s) 149, 1134, 2438
Hsp92I GRCGYC 1 cut(s) 1994
HspAI GCGC 5 cut(s) 890, 2769, 3146, 3354, 3356
Kpn2I TCCGGA 3 cut(s) 207, 275, 1630
Ksp22I TGATCA 3 cut(s) 945, 1848, 2346
KspAI GTTAAC 2 cut(s) 1159, 3697
KspI CCGCGG 1 cut(s) 105
MaeII ACGT 3 cut(s) 149, 1134, 2438
MaeIII GTNAC 6 cut(s) 79, 150, 730, 1033, 2546, 2644
MfeI CAATTG 1 cut(s) 1959
MflI RGATCY 3 cut(s) 718, 2123, 2187
MhlI GDGCHC 8 cut(s) 183, 798, 1078, 1974, 2394, 2756, 3166, 3757
MlsI TGGCCA 2 cut(s) 1272, 3770
MluNI TGGCCA 2 cut(s) 1272, 3770
MlyI GAGTC 4 cut(s) 266, 3019, 3579, 3746
MmeI TCCRAC 5 cut(s) 401, 914, 2170, 2841, 2921
Mox20I TGGCCA 2 cut(s) 1272, 3770
Mph1103I ATGCAT 2 cut(s) 2144, 3313
MroI TCCGGA 3 cut(s) 207, 275, 1630
MroXI GAANNNNTTC 4 cut(s) 926, 2102, 2300, 3618
MscI TGGCCA 2 cut(s) 1272, 3770
MslI CAYNNNNRTG 6 cut(s) 548, 1299, 2561, 2811, 3391, 3690
Msp20I TGGCCA 2 cut(s) 1272, 3770
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 9 cut(s) 208, 276, 379, 433, 799, 1631, 1869, 2701, 2732
MspR9I CCNGG 7 cut(s) 379, 380, 1274, 1746, 1989, 2322, 3810
MunI CAATTG 1 cut(s) 1959
Mva1269I GAATGC 3 cut(s) 230, 915, 1008
MvaI CCWGG 5 cut(s) 1274, 1746, 1989, 2322, 3810
MvnI CGCG 3 cut(s) 104, 892, 3356
NciI CCSGG 2 cut(s) 379, 380
NcoI CCATGG 2 cut(s) 1576, 3685
NdeI CATATG 1 cut(s) 1648
NlaIV GGNNCC 2 cut(s) 720, 1564
NmeAIII GCCGAG 1 cut(s) 2317
NmuCI GTSAC 1 cut(s) 1033
NsbI TGCGCA 1 cut(s) 2770
NsiI ATGCAT 2 cut(s) 2144, 3313
NspI RCATGY 5 cut(s) 175, 559, 959, 1165, 2566
OliI CACNNNNGTG 1 cut(s) 3391
PaeR7I CTCGAG 2 cut(s) 3389, 3748
PagI TCATGA 2 cut(s) 942, 2806
PaqCI CACCTGC 1 cut(s) 2590
PauI GCGCGC 1 cut(s) 3354
PciI ACATGT 4 cut(s) 555, 955, 1161, 2562
PctI GAATGC 3 cut(s) 230, 915, 1008
PdmI GAANNNNTTC 4 cut(s) 926, 2102, 2300, 3618
PflMI CCANNNNNTGG 1 cut(s) 2273
PfoI TCCNGGA 1 cut(s) 1744
PinAI ACCGGT 2 cut(s) 432, 798
PleI GAGTC 4 cut(s) 266, 3018, 3579, 3745
PpsI GAGTC 4 cut(s) 266, 3018, 3579, 3745
Ppu21I YACGTR 1 cut(s) 2439
PscI ACATGT 4 cut(s) 555, 955, 1161, 2562
PshBI ATTAAT 1 cut(s) 3831
PsiI TTATAA 1 cut(s) 696
Psp124BI GAGCTC 3 cut(s) 183, 1078, 2394
Psp6I CCWGG 5 cut(s) 1272, 1744, 1987, 2320, 3808
PspFI CCCAGC 2 cut(s) 198, 1373
PspGI CCWGG 5 cut(s) 1272, 1744, 1987, 2320, 3808
PspN4I GGNNCC 2 cut(s) 720, 1564
PspPI GGNCC 2 cut(s) 376, 2424
PspXI VCTCGAGB 2 cut(s) 3389, 3748
PstI CTGCAG 2 cut(s) 1571, 1597
PstNI CAGNNNCTG 1 cut(s) 3788
PsuI RGATCY 3 cut(s) 718, 2123, 2187
PteI GCGCGC 1 cut(s) 3354
RsaI GTAC 9 cut(s) 533, 560, 815, 844, 1166, 1981, 2441, 3060, 3247
RsaNI GTAC 9 cut(s) 532, 559, 814, 843, 1165, 1980, 2440, 3059, 3246
RseI CAYNNNNRTG 6 cut(s) 548, 1299, 2561, 2811, 3391, 3690
SacI GAGCTC 3 cut(s) 183, 1078, 2394
SacII CCGCGG 1 cut(s) 105
Sau96I GGNCC 2 cut(s) 376, 2424
SchI GAGTC 4 cut(s) 266, 3019, 3579, 3746
ScrFI CCNGG 7 cut(s) 379, 380, 1274, 1746, 1989, 2322, 3810
SduI GDGCHC 8 cut(s) 183, 798, 1078, 1974, 2394, 2756, 3166, 3757
SfcI CTRYAG 3 cut(s) 1567, 1593, 2742
SfiI GGCCNNNNNGGCC 1 cut(s) 383
Sfr274I CTCGAG 2 cut(s) 3389, 3748
Sfr303I CCGCGG 1 cut(s) 105
SgrAI CRCCGGYG 1 cut(s) 798
SgrBI CCGCGG 1 cut(s) 105
SlaI CTCGAG 2 cut(s) 3389, 3748
SmaI CCCGGG 1 cut(s) 380
SmiMI CAYNNNNRTG 6 cut(s) 548, 1299, 2561, 2811, 3391, 3690
SmlI CTYRAG 7 cut(s) 182, 1010, 1586, 2153, 3389, 3454, 3748
SmoI CTYRAG 7 cut(s) 182, 1010, 1586, 2153, 3389, 3454, 3748
SpeI ACTAGT 2 cut(s) 2837, 2945
SspI AATATT 1 cut(s) 1732
SstI GAGCTC 3 cut(s) 183, 1078, 2394
StyD4I CCNGG 7 cut(s) 377, 378, 1272, 1744, 1987, 2320, 3808
StyI CCWWGG 2 cut(s) 1576, 3685
TaaI ACNGT 8 cut(s) 85, 215, 660, 818, 1538, 2746, 3139, 3659
TaiI ACGT 3 cut(s) 152, 1137, 2441
TatI WGTACW 4 cut(s) 558, 813, 1979, 3058
TauI GCSGC 3 cut(s) 104, 107, 110
TscAI CASTG 9 cut(s) 665, 1218, 2035, 2047, 2694, 2781, 3090, 3631, 3676
TseFI GTSAC 1 cut(s) 1033
Tsp45I GTSAC 1 cut(s) 1033
TspGWI ACGGA 2 cut(s) 3216, 3896
TspMI CCCGGG 1 cut(s) 378
TspRI CASTG 9 cut(s) 665, 1218, 2035, 2047, 2694, 2781, 3090, 3631, 3676
Van91I CCANNNNNTGG 1 cut(s) 2273
VneI GTGCAC 1 cut(s) 794
VspI ATTAAT 1 cut(s) 3831
XagI CCTNNNNNAGG 1 cut(s) 3593
XapI RAATTY 4 cut(s) 1784, 2802, 2916, 3131
XbaI TCTAGA 1 cut(s) 1078
XceI RCATGY 5 cut(s) 175, 559, 959, 1165, 2566
XcmI CCANNNNNNNNNTGG 1 cut(s) 2467
XhoI CTCGAG 2 cut(s) 3389, 3748
XmaI CCCGGG 1 cut(s) 378
XmnI GAANNNNTTC 4 cut(s) 926, 2102, 2300, 3618
Zsp2I ATGCAT 2 cut(s) 2144, 3313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.