Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0005071

Physical Location & Seq

Genomic Coordinates Reverse (-)

3198430 .. 3200652

Gene Structure 2223 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G003400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2223 bp

ATGGTGTTTTCGTTTTCATCTTCGTCTTCGTTGCTGACCGCTCCACCCTCCACCCTTCACGTCCTCCCCGCCTCCGATCCTCCGTACAACCTCCTCCAAACCCACCCATCTCTCACTCTCCTCTCCAAATGCAGAAGCATCCAAAATCTCAAACAAGTCCACACCCACATCATCAAGACCGGCCTCCACAACACCCACTTTGCTATGAGCAAGCTCATTGAGTTTTGTGCCGTTTCGCAATTTGGGGACCTCTCCTATGCTCTGTCGGTCTTCGAATGCGTTGAGAATCCGAACCAGATTATTTGGAACACAATTATTCGAGGCTATTCCTTGAGCTCGAAGCCATTACAGGCCGTGGAGTTCTATGTAAGGATGATTGTTTCGGGTGCGGAGCCGAATTCTTACACATTCCCTTTTGTTTTGAAGTCTTGTGCGAAAGCTGGGGCAGCTCAGGAAGGGAAGCAGGTCCATGGGCATGTGTTGAAGCATGGAGTTGGCTCTGATGCGTTTGTGCACACTTCTCTGATCAACATGTATGCGAAAAGTGGTGAATTGGATAATGCAAGGTTGGTTTTTGATAAAAGTCATCTGAGAGATGCGGTTTCGTTTACTGCTTTGATTACTGGCTATGTTTCAAGGGGTTGTATGGATGATGCCCGCAGCTTGTTTGATGAAATTTTAGTAAGAGATGCGGTGTCTTGGAATGCTATGATTTCAGGGTATGCTCAGAGCGGCAGGTTTGAGGAAGCGCTGGCTTTGTTTTCGGATATGCGAAAAGCAAATGTGGTACCGAATGAGAGTACTATGCTTACTGTGCTTTCTGCTTGTGCTCAGTCGGGGTCACTTGAATTGGGAAAATGGGTCGCTTCTTGGATTGAAGACCAGGGACTTGGATCGAATGTTAGGCTTGTTAATGCACTTATTGATATGTACTCAAAGTGTGGTGCTTTGGACACAGCTAGAGGTTTATTTGATGGTATGCAACAGAGGGATGTGATCTCATGGAATGTTATGATTGGTGGTTATACACATAAGAGCCAGTACAAGGAAGCCTTGGCACTCTTTCGGTTAATGCTACGTTCAAATGCAGAGCCTAATGATGTCACATTCTTGGGCATTCTTCCGGCTTGTGCTCATTTGGGAGCTCTTGACCTTGGCAAATGGATTCATGTTTATATAGATAAGAAGTTGCAGTGTTTGACCAATACATCTCTCCAGACCAGTCTCATAGATATGTATGCAAAATGCGGAAACATTGAGGCAGCAAAACAAGTCTTTGATGGTATGGAAGCCAAAAGCTTGGCCTCTTGGAATGCAATGATAACTGGCTTAGCAATGCATGGGAAAGCAAATGCAGCACTTGAGCTTTTCTCGAAGATGGCTGATGAAGGATTCAAACCAGATGATATCACATTTGTGGGGGTTTTATCGGCTTGCAACCATGGTGGTCTAGTAGACCTTGGACGCCAATATTTTAGTTCCATGACCAAAGACTACCATATTCCACCGCAACTACAACACTATGGATGTATGATAGATCTTCTAGGACGAGCTGGGCTACTTGATGAAGCAGAGGCCCTCATGAAGACCATGGAGATGAAGCCAGATGGTGCTGTTTGGGGTTCTCTTCTTGGGGCTTGTAGAACCCATAAGCGTGTAGAGATGGGTGAATATGTTGCCAAACATGTGTTCGAATTGGAGCCTGAAAATGCTGGGGCTCATGTGCTGCTATCAAATATCTATGCAAGTGCTGGTAGGTGGGATGATGTAGCCAGAATAAGAACCACGCTGAACGATATGGGAATCAAGAAAGTTCCTGGCAGTACTTCTATTGAGGTGGATAGTGTTGTTCATGAGTTTCTCGTTGGTGACAAAGTGCATCCTTTAAGTGAAGAAATCTATGAGATGTTGAAAGAAATGGATAGGCTTTTGGATATGGCTGGTTTTGTGCCTGATACATCTGAGGTACTCTATGATATGGATGAGGAATGGAAGGAAGGGATTTTGAGTCATCATAGTGAGAAGTTGGCCATTGCTTTTGGTTTGATCAGCACAAAGCCAGGCACAACTATTCGAATTGTAAAGAATCTTCGAGTATGTGGAAATTGTCATTCAGCTACAAAGCTGATATCCAAGATCTTCAATAGGGAAATTATTGCAAGAGATAGGAATCGCTTCCACCATTTTAGAGATGGTTCTTGTTCATGTAATGACAATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0016070 GO:0016787 GO:0016788 GO:0031425 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1900865 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

740

Amino Acids

81.75

Weight (kDa)

6.22

Isoelectric Point (pI)

38.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	97 - 146	1.4e-08	PPR repeat family
PPR_1	PF12854	228 - 258	2e-06	PPR repeat
PPR_2	PF13041	230 - 277	3.6e-14	PPR repeat family
PPR	PF01535	232 - 261	4.3e-10	PPR repeat
PPR_2	PF13041	330 - 379	6.7e-11	PPR repeat family
PPR	PF01535	333 - 361	7.2e-06	PPR repeat
PPR_2	PF13041	435 - 479	1.5e-10	PPR repeat family
PPR	PF01535	436 - 465	3.7e-06	PPR repeat
E_motif	PF20431	550 - 612	2.3e-19	E motif
Eplus_motif	PF20430	617 - 639	1.4e-07	E+ motif
DYW_deaminase	PF14432	648 - 740	1.8e-40	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005071)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08070 AT1G08070
fragaria_vesca	FvH4_1g03710 FvH4_1g04070
malus_domestica	MD02G1039500.v1.1 MD15G1176200.v1.1 MD15G1180300.v1.1
prunus_persica	Prupe.7G236200_v2.0.a1 Prupe.7G239300_v2.0.a1
pyrus_communis	pycom02g03280 pycom15g15800 pycom15g16170 pycom15g16180
rosa_chinensis	RchiOBHm_Chr2g0089161 RchiOBHm_Chr2g0089561
rosa_laevigata	RLG00000015999 RLG00000016040
rosa_multiflora	Rmu_co8347093.1_g000001 Rmu_sc0006193.1_g000005 Rmu_sc0008909.1_g000002 Rmu_sc0023591.1_g000001
rosa_rugosa	Rorug01G0488000 Rorug02G0001500
rosa_samantha	Rh2AG043600 Rh2BG042300 Rh2CG043600 Rh2CG047500 Rh2DG042700
rosa_wichuraiana	Rw2G003400 Rw2G003590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	454, 726
Acc65I	GGTACC	1 cut(s)	787
AccB1I	GGYRCC	1 cut(s)	787
AccBSI	CCGCTC	2 cut(s)	41, 732
AccI	GTMKAC	1 cut(s)	1455
AciI	CCGC	9 cut(s)	39, 69, 389, 599, 658, 692, 732, 1248, 1508
AclWI	GGATC	2 cut(s)	71, 901
AcoI	YGGCCR	1 cut(s)	2028
AcsI	RAATTY	2 cut(s)	397, 675
AcyI	GRCGYC	1 cut(s)	1465
AfaI	GTAC	7 cut(s)	86, 789, 802, 932, 1043, 1825, 1968
AfeI	AGCGCT	1 cut(s)	750
AfiI	CCNNNNNNNGG	1 cut(s)	1045
AflIII	ACRYGT	2 cut(s)	531, 1684
AgsI	TTSAA	9 cut(s)	424, 484, 636, 848, 878, 1083, 1396, 1912, 2143
AjiI	CACGTC	1 cut(s)	61
AjnI	CCWGG	3 cut(s)	882, 1816, 2059
AjuI	GAANNNNNNNTTGG	2 cut(s)	1673, 1705
AleI	CACNNNNGTG	1 cut(s)	1415
Alw21I	GWGCWC	5 cut(s)	338, 516, 832, 1135, 1147
Alw26I	GTCTC	1 cut(s)	1229
Alw44I	GTGCAC	1 cut(s)	512
AlwI	GGATC	2 cut(s)	71, 901
Aor51HI	AGCGCT	1 cut(s)	750
AoxI	GGCC	5 cut(s)	181, 351, 1302, 1575, 2028
ApaLI	GTGCAC	1 cut(s)	512
ApeKI	GCWGC	5 cut(s)	446, 660, 1262, 1355, 1726
ApoI	RAATTY	2 cut(s)	397, 675
Asp700I	GAANNNNTTC	1 cut(s)	2174
Asp718I	GGTACC	1 cut(s)	787
AspLEI	GCGC	1 cut(s)	751
AspS9I	GGNCC	3 cut(s)	247, 466, 1576
AsuHPI	GGTGA	3 cut(s)	560, 1679, 1880
AsuII	TTCGAA	3 cut(s)	273, 1692, 2074
AvaII	GGWCC	2 cut(s)	247, 466
BaeGI	GKGCMC	1 cut(s)	516
BalI	TGGCCA	1 cut(s)	2030
BanI	GGYRCC	1 cut(s)	787
BanII	GRGCYC	3 cut(s)	338, 1147, 1720
BarI	GAAGNNNNNNTAC	2 cut(s)	2073, 2105
BbsI	GAAGAC	4 cut(s)	18, 262, 885, 1592
Bbv12I	GWGCWC	5 cut(s)	338, 516, 832, 1135, 1147
BbvI	GCAGC	5 cut(s)	458, 672, 1274, 1367, 1713
BccI	CCATC	7 cut(s)	115, 968, 1274, 1372, 1601, 1657, 2186
BceAI	ACGGC	2 cut(s)	215, 338
BciT130I	CCWGG	3 cut(s)	884, 1818, 2061
BclI	TGATCA	2 cut(s)	525, 2046
BcoDI	GTCTC	1 cut(s)	1229
BfaI	CTAG	3 cut(s)	960, 1451, 1544
BfoI	RGCGCY	1 cut(s)	752
BfuAI	ACCTGC	2 cut(s)	454, 726
BglII	AGATCT	2 cut(s)	1537, 2136
BisI	GCNGC	6 cut(s)	447, 661, 733, 1263, 1356, 1727
BlpI	GCTNAGC	1 cut(s)	1330
BlsI	GCNGC	6 cut(s)	448, 662, 734, 1264, 1357, 1728
BmcAI	AGTACT	2 cut(s)	802, 1825
Bme1390I	CCNGG	3 cut(s)	884, 1818, 2061
Bme18I	GGWCC	2 cut(s)	247, 466
BmgBI	CACGTC	1 cut(s)	61
BmgT120I	GGNCC	3 cut(s)	247, 466, 1576
BmiI	GGNNCC	4 cut(s)	248, 393, 789, 1701
BmrFI	CCNGG	3 cut(s)	884, 1818, 2061
BmsI	GCATC	6 cut(s)	147, 493, 586, 643, 679, 1888
BpiI	GAAGAC	4 cut(s)	18, 262, 885, 1592
BplI	GAGNNNNNCTC	2 cut(s)	1355, 1387
BpmI	CTGGAG	1 cut(s)	1199
Bpu10I	CCTNAGC	1 cut(s)	450
Bpu1102I	GCTNAGC	1 cut(s)	1330
Bpu14I	TTCGAA	3 cut(s)	273, 1692, 2074
BpuEI	CTTGAG	2 cut(s)	352, 1382
BsaBI	GATNNNNATC	1 cut(s)	2169
BsaHI	GRCGYC	1 cut(s)	1465
BsaJI	CCNNGG	8 cut(s)	354, 469, 883, 1053, 1153, 1441, 1459, 1590
Bsc4I	CCNNNNNNNGG	1 cut(s)	1045
Bse118I	RCCGGY	1 cut(s)	179
Bse1I	ACTGG	4 cut(s)	628, 1039, 1221, 1330
Bse3DI	GCAATG	3 cut(s)	1323, 1341, 2031
Bse8I	GATNNNNATC	1 cut(s)	2169
BseBI	CCWGG	3 cut(s)	884, 1818, 2061
BseDI	CCNNGG	8 cut(s)	354, 469, 883, 1053, 1153, 1441, 1459, 1590
BseGI	GGATG	8 cut(s)	138, 378, 655, 997, 1532, 1768, 1879, 1987
BseJI	GATNNNNATC	1 cut(s)	2169
BseLI	CCNNNNNNNGG	1 cut(s)	1045
BseMI	GCAATG	3 cut(s)	1323, 1341, 2031
BseMII	CTCAG	5 cut(s)	464, 581, 740, 845, 1953
BseNI	ACTGG	4 cut(s)	628, 1039, 1221, 1330
BseRI	GAGGAG	2 cut(s)	83, 110
BseSI	GKGCMC	1 cut(s)	516
BseXI	GCAGC	5 cut(s)	458, 672, 1274, 1367, 1713
BseYI	CCCAGC	3 cut(s)	440, 1553, 1712
BshFI	GGCC	5 cut(s)	183, 353, 1304, 1577, 2030
BshNI	GGYRCC	1 cut(s)	787
BsiHKAI	GWGCWC	5 cut(s)	338, 516, 832, 1135, 1147
BsiSI	CCGG	2 cut(s)	180, 1124
BslFI	GGGAC	2 cut(s)	260, 900
BslI	CCNNNNNNNGG	1 cut(s)	1045
BsmAI	GTCTC	1 cut(s)	1229
BsmFI	GGGAC	2 cut(s)	260, 900
BsmI	GAATGC	4 cut(s)	281, 709, 1116, 1318
BsnI	GGCC	5 cut(s)	183, 353, 1304, 1577, 2030
Bsp119I	TTCGAA	3 cut(s)	273, 1692, 2074
Bsp1286I	GDGCHC	6 cut(s)	338, 516, 832, 1135, 1147, 1720
Bsp143I	GATC	7 cut(s)	76, 525, 893, 996, 1537, 2046, 2136
Bsp1720I	GCTNAGC	1 cut(s)	1330
Bsp19I	CCATGG	3 cut(s)	469, 1441, 1590
BspACI	CCGC	9 cut(s)	39, 69, 389, 599, 658, 692, 732, 1248, 1508
BspANI	GGCC	5 cut(s)	183, 353, 1304, 1577, 2030
BspCNI	CTCAG	5 cut(s)	463, 582, 739, 844, 1954
BspHI	TCATGA	2 cut(s)	1581, 1852
BspLI	GGNNCC	4 cut(s)	248, 393, 789, 1701
BspMI	ACCTGC	2 cut(s)	454, 726
BspPI	GGATC	2 cut(s)	71, 901
BspT104I	TTCGAA	3 cut(s)	273, 1692, 2074
BspT107I	GGYRCC	1 cut(s)	787
BsrBI	CCGCTC	2 cut(s)	41, 732
BsrDI	GCAATG	3 cut(s)	1323, 1341, 2031
BsrFI	RCCGGY	1 cut(s)	179
BsrI	ACTGG	4 cut(s)	628, 1039, 1221, 1330
BssAI	RCCGGY	1 cut(s)	179
BssECI	CCNNGG	8 cut(s)	354, 469, 883, 1053, 1153, 1441, 1459, 1590
BssMI	GATC	7 cut(s)	76, 525, 893, 996, 1537, 2046, 2136
BssNI	GRCGYC	1 cut(s)	1465
BssT1I	CCWWGG	6 cut(s)	469, 1053, 1153, 1441, 1459, 1590
Bst2UI	CCWGG	3 cut(s)	884, 1818, 2061
Bst4CI	ACNGT	1 cut(s)	814
Bst6I	CTCTTC	1 cut(s)	1632
BstACI	GRCGYC	1 cut(s)	1465
BstBI	TTCGAA	3 cut(s)	273, 1692, 2074
BstC8I	GCNNGC	4 cut(s)	212, 658, 753, 1435
BstDEI	CTNAG	6 cut(s)	450, 590, 726, 831, 1330, 1962
BstDSI	CCRYGG	4 cut(s)	354, 469, 1441, 1590
BstF5I	GGATG	8 cut(s)	138, 378, 655, 997, 1532, 1768, 1879, 1987
BstH2I	RGCGCY	1 cut(s)	752
BstHHI	GCGC	1 cut(s)	751
BstKTI	GATC	7 cut(s)	79, 528, 896, 999, 1540, 2049, 2139
BstMAI	GTCTC	1 cut(s)	1229
BstMBI	GATC	7 cut(s)	76, 525, 893, 996, 1537, 2046, 2136
BstMWI	GCNNNNNNNGC	3 cut(s)	446, 814, 1355
BstNI	CCWGG	3 cut(s)	884, 1818, 2061
BstNSI	RCATGY	3 cut(s)	479, 535, 1688
BstSCI	CCNGG	3 cut(s)	882, 1816, 2059
BstSLI	GKGCMC	1 cut(s)	516
BstV1I	GCAGC	5 cut(s)	458, 672, 1274, 1367, 1713
BstV2I	GAAGAC	4 cut(s)	18, 262, 885, 1592
BstX2I	RGATCY	2 cut(s)	1537, 2136
BstXI	CCANNNNNNTGG	2 cut(s)	890, 1300
BstYI	RGATCY	2 cut(s)	1537, 2136
BsuRI	GGCC	5 cut(s)	183, 353, 1304, 1577, 2030
BtgI	CCRYGG	4 cut(s)	354, 469, 1441, 1590
BtrI	CACGTC	1 cut(s)	61
BtsCI	GGATG	8 cut(s)	138, 378, 655, 997, 1532, 1768, 1879, 1987
BtsI	GCAGTG	1 cut(s)	1199
BtsIMutI	CAGTG	1 cut(s)	1199
BveI	ACCTGC	2 cut(s)	454, 726
Cac8I	GCNNGC	4 cut(s)	212, 658, 753, 1435
CciI	TCATGA	2 cut(s)	1581, 1852
CfoI	GCGC	1 cut(s)	751
Cfr10I	RCCGGY	1 cut(s)	179
Cfr13I	GGNCC	3 cut(s)	247, 466, 1576
CseI	GACGC	1 cut(s)	1473
Csp6I	GTAC	7 cut(s)	85, 788, 801, 931, 1042, 1824, 1967
CspCI	CAANNNNNGTGG	2 cut(s)	1426, 1461
CviQI	GTAC	7 cut(s)	85, 788, 801, 931, 1042, 1824, 1967
DdeI	CTNAG	6 cut(s)	450, 590, 726, 831, 1330, 1962
DpnI	GATC	7 cut(s)	78, 527, 895, 998, 1539, 2048, 2138
DpnII	GATC	7 cut(s)	76, 525, 893, 996, 1537, 2046, 2136
EaeI	YGGCCR	1 cut(s)	2028
Eam1104I	CTCTTC	1 cut(s)	1632
EarI	CTCTTC	1 cut(s)	1632
Ecl136II	GAGCTC	2 cut(s)	336, 1145
Eco130I	CCWWGG	6 cut(s)	469, 1053, 1153, 1441, 1459, 1590
Eco24I	GRGCYC	3 cut(s)	338, 1147, 1720
Eco32I	GATATC	2 cut(s)	1408, 2130
Eco47I	GGWCC	2 cut(s)	247, 466
Eco47III	AGCGCT	1 cut(s)	750
Eco53kI	GAGCTC	2 cut(s)	336, 1145
EcoICRI	GAGCTC	2 cut(s)	336, 1145
EcoO109I	RGGNCCY	2 cut(s)	247, 1576
EcoRI	GAATTC	1 cut(s)	397
EcoRII	CCWGG	3 cut(s)	882, 1816, 2059
EcoRV	GATATC	2 cut(s)	1408, 2130
EcoT14I	CCWWGG	6 cut(s)	469, 1053, 1153, 1441, 1459, 1590
EcoT22I	ATGCAT	1 cut(s)	1341
EcoT38I	GRGCYC	3 cut(s)	338, 1147, 1720
ErhI	CCWWGG	6 cut(s)	469, 1053, 1153, 1441, 1459, 1590
FalI	AAGNNNNNCTT	2 cut(s)	1037, 1069
FaqI	GGGAC	2 cut(s)	260, 900
FauI	CCCGC	2 cut(s)	76, 665
FbaI	TGATCA	2 cut(s)	525, 2046
FblI	GTMKAC	1 cut(s)	1455
Fnu4HI	GCNGC	6 cut(s)	447, 661, 733, 1263, 1356, 1727
FokI	GGATG	8 cut(s)	125, 385, 662, 1004, 1539, 1775, 1866, 1994
FriOI	GRGCYC	3 cut(s)	338, 1147, 1720
Fsp4HI	GCNGC	6 cut(s)	447, 661, 733, 1263, 1356, 1727
FspBI	CTAG	3 cut(s)	960, 1451, 1544
GlaI	GCGC	1 cut(s)	750
GluI	GCNGC	6 cut(s)	447, 661, 733, 1263, 1356, 1727
GsaI	CCCAGC	3 cut(s)	444, 1557, 1716
GsuI	CTGGAG	1 cut(s)	1199
HaeII	RGCGCY	1 cut(s)	752
HaeIII	GGCC	5 cut(s)	183, 353, 1304, 1577, 2030
HapII	CCGG	2 cut(s)	180, 1124
HgaI	GACGC	1 cut(s)	1473
HhaI	GCGC	1 cut(s)	751
Hin1I	GRCGYC	1 cut(s)	1465
Hin6I	GCGC	1 cut(s)	749
HinP1I	GCGC	1 cut(s)	749
HindIII	AAGCTT	1 cut(s)	1297
HinfI	GANTC	7 cut(s)	286, 1165, 1392, 1803, 2008, 2086, 2170
HpaII	CCGG	2 cut(s)	180, 1124
HphI	GGTGA	3 cut(s)	560, 1679, 1880
Hpy166II	GTNNAC	4 cut(s)	160, 514, 609, 1456
Hpy188I	TCNGA	8 cut(s)	76, 291, 502, 525, 591, 729, 766, 1963
Hpy188III	TCNNGA	8 cut(s)	175, 452, 1148, 1216, 1372, 1582, 1807, 1853
Hpy8I	GTNNAC	4 cut(s)	160, 514, 609, 1456
HpyAV	CCTTC	5 cut(s)	65, 449, 1382, 1987, 1991
HpyCH4III	ACNGT	1 cut(s)	814
HpyCH4IV	ACGT	2 cut(s)	60, 1078
HpyF10VI	GCNNNNNNNGC	3 cut(s)	446, 814, 1355
HpyF3I	CTNAG	6 cut(s)	450, 590, 726, 831, 1330, 1962
HpySE526I	ACGT	2 cut(s)	60, 1078
Hsp92I	GRCGYC	1 cut(s)	1465
HspAI	GCGC	1 cut(s)	749
KpnI	GGTACC	1 cut(s)	791
Ksp22I	TGATCA	2 cut(s)	525, 2046
Kzo9I	GATC	7 cut(s)	76, 525, 893, 996, 1537, 2046, 2136
LmnI	GCTCC	4 cut(s)	46, 391, 1142, 1699
Lsp1109I	GCAGC	5 cut(s)	458, 672, 1274, 1367, 1713
LweI	GCATC	6 cut(s)	147, 493, 586, 643, 679, 1888
MaeI	CTAG	3 cut(s)	960, 1451, 1544
MaeII	ACGT	2 cut(s)	60, 1078
MaeIII	GTNAC	3 cut(s)	840, 1102, 1868
MalI	GATC	7 cut(s)	78, 527, 895, 998, 1539, 2048, 2138
MbiI	CCGCTC	2 cut(s)	41, 732
MboI	GATC	7 cut(s)	76, 525, 893, 996, 1537, 2046, 2136
MfeI	CAATTG	1 cut(s)	2215
MflI	RGATCY	2 cut(s)	1537, 2136
MhlI	GDGCHC	6 cut(s)	338, 516, 832, 1135, 1147, 1720
MlsI	TGGCCA	1 cut(s)	2030
MluNI	TGGCCA	1 cut(s)	2030
MlyI	GAGTC	1 cut(s)	2017
Mox20I	TGGCCA	1 cut(s)	2030
Mph1103I	ATGCAT	1 cut(s)	1341
MroXI	GAANNNNTTC	1 cut(s)	2174
MscI	TGGCCA	1 cut(s)	2030
MseI	TTAA	3 cut(s)	912, 1070, 1886
MslI	CAYNNNNRTG	6 cut(s)	474, 1232, 1415, 1595, 1653, 2016
Msp20I	TGGCCA	1 cut(s)	2030
MspI	CCGG	2 cut(s)	180, 1124
MspR9I	CCNGG	3 cut(s)	884, 1818, 2061
MunI	CAATTG	1 cut(s)	2215
Mva1269I	GAATGC	4 cut(s)	281, 709, 1116, 1318
MvaI	CCWGG	3 cut(s)	884, 1818, 2061
MwoI	GCNNNNNNNGC	3 cut(s)	446, 814, 1355
NcoI	CCATGG	3 cut(s)	469, 1441, 1590
NdeII	GATC	7 cut(s)	76, 525, 893, 996, 1537, 2046, 2136
NlaIV	GGNNCC	4 cut(s)	248, 393, 789, 1701
NmuCI	GTSAC	3 cut(s)	840, 1102, 1868
NsiI	ATGCAT	1 cut(s)	1341
NspI	RCATGY	3 cut(s)	479, 535, 1688
NspV	TTCGAA	3 cut(s)	273, 1692, 2074
OliI	CACNNNNGTG	1 cut(s)	1415
PagI	TCATGA	2 cut(s)	1581, 1852
PciI	ACATGT	2 cut(s)	531, 1684
PctI	GAATGC	4 cut(s)	281, 709, 1116, 1318
PdmI	GAANNNNTTC	1 cut(s)	2174
PfeI	GAWTC	6 cut(s)	286, 1165, 1392, 1803, 2086, 2170
PkrI	GCNGC	6 cut(s)	448, 662, 734, 1264, 1357, 1728
PleI	GAGTC	1 cut(s)	2016
PpsI	GAGTC	1 cut(s)	2016
PpuMI	RGGWCCY	1 cut(s)	247
PscI	ACATGT	2 cut(s)	531, 1684
Psp124BI	GAGCTC	2 cut(s)	338, 1147
Psp5II	RGGWCCY	1 cut(s)	247
Psp6I	CCWGG	3 cut(s)	882, 1816, 2059
PspFI	CCCAGC	3 cut(s)	440, 1553, 1712
PspGI	CCWGG	3 cut(s)	882, 1816, 2059
PspN4I	GGNNCC	4 cut(s)	248, 393, 789, 1701
PspPI	GGNCC	3 cut(s)	247, 466, 1576
PspPPI	RGGWCCY	1 cut(s)	247
PsuI	RGATCY	2 cut(s)	1537, 2136
RsaI	GTAC	7 cut(s)	86, 789, 802, 932, 1043, 1825, 1968
RsaNI	GTAC	7 cut(s)	85, 788, 801, 931, 1042, 1824, 1967
RseI	CAYNNNNRTG	6 cut(s)	474, 1232, 1415, 1595, 1653, 2016
SacI	GAGCTC	2 cut(s)	338, 1147
SaqAI	TTAA	3 cut(s)	912, 1070, 1886
SatI	GCNGC	6 cut(s)	447, 661, 733, 1263, 1356, 1727
Sau3AI	GATC	7 cut(s)	76, 525, 893, 996, 1537, 2046, 2136
Sau96I	GGNCC	3 cut(s)	247, 466, 1576
ScaI	AGTACT	2 cut(s)	802, 1825
SchI	GAGTC	1 cut(s)	2017
ScrFI	CCNGG	3 cut(s)	884, 1818, 2061
SduI	GDGCHC	6 cut(s)	338, 516, 832, 1135, 1147, 1720
SfaNI	GCATC	6 cut(s)	147, 493, 586, 643, 679, 1888
SfuI	TTCGAA	3 cut(s)	273, 1692, 2074
SinI	GGWCC	2 cut(s)	247, 466
SmiMI	CAYNNNNRTG	6 cut(s)	474, 1232, 1415, 1595, 1653, 2016
SmlI	CTYRAG	2 cut(s)	331, 1361
SmoI	CTYRAG	2 cut(s)	331, 1361
SsiI	CCGC	9 cut(s)	39, 69, 389, 599, 658, 692, 732, 1248, 1508
SspI	AATATT	1 cut(s)	1472
SspMI	CTAG	3 cut(s)	960, 1451, 1544
SstI	GAGCTC	2 cut(s)	338, 1147
StyD4I	CCNGG	3 cut(s)	882, 1816, 2059
StyI	CCWWGG	6 cut(s)	469, 1053, 1153, 1441, 1459, 1590
TaaI	ACNGT	1 cut(s)	814
TaiI	ACGT	2 cut(s)	63, 1081
TaqI	TCGA	8 cut(s)	273, 319, 338, 896, 1373, 1692, 2074, 2092
TaqII	GACCGA	1 cut(s)	256
TatI	WGTACW	4 cut(s)	800, 930, 1041, 1823
TauI	GCSGC	1 cut(s)	735
TfiI	GAWTC	6 cut(s)	286, 1165, 1392, 1803, 2086, 2170
Tru1I	TTAA	3 cut(s)	912, 1070, 1886
Tru9I	TTAA	3 cut(s)	912, 1070, 1886
TscAI	CASTG	1 cut(s)	1199
TseFI	GTSAC	3 cut(s)	840, 1102, 1868
TseI	GCWGC	5 cut(s)	446, 660, 1262, 1355, 1726
Tsp45I	GTSAC	3 cut(s)	840, 1102, 1868
TspDTI	ATGAA	9 cut(s)	6, 687, 1157, 1401, 1581, 1598, 1613, 1841, 2193
TspGWI	ACGGA	1 cut(s)	72
TspRI	CASTG	1 cut(s)	1199
VneI	GTGCAC	1 cut(s)	512
VpaK11BI	GGWCC	2 cut(s)	247, 466
XapI	RAATTY	2 cut(s)	397, 675
XceI	RCATGY	3 cut(s)	479, 535, 1688
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2189
XmiI	GTMKAC	1 cut(s)	1455
XmnI	GAANNNNTTC	1 cut(s)	2174
XspI	CTAG	3 cut(s)	960, 1451, 1544
ZrmI	AGTACT	2 cut(s)	802, 1825
Zsp2I	ATGCAT	1 cut(s)	1341

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.