pycom15g18090

Pollen-specific protein SF21-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001208
Physical Location & Seq
Reverse (-)
12834132 .. 12837842
3711 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1044 bp
ATGGCTGAATCAAACGACGCCGTCTCCCTCGATATGGAGAAGATCTATCTCGGTGGAAAGGAACATTTTATTCGAACTGGCTGTGGTTCAGTGTCTGTTATCGTGTATGGAGACCAAGAGAAGCCTGCACTTATCACTTATCCTGATTTAGCTCTAAATCATGTGTCTTGTTTCCAAGGGTTATTCTTTTGTCCGGAAGCGGCTTCTTTGCTGCTCCACAATTTCTGCATATATCATATCAGTCCTCCTGGCCATGAGTTAGGAGCTGCTTCAGTTTGTCCAGAGGATCCTGTGCCTTCAGTTGATGACTTGGCAGATCAGATCCTTGAGGTTCTCAATTTTTTTGGGCTTGGTGCAGTTATGTGCATGGGGGTGACAGCGGGTGCTTATATCCTTTCCCTATTTGCTATGAAATATAGGGAGCGTGTCCTTGGATTGATACTTGTATCCCCTTTATGCAAAGCGCCCTCTTGGACAGAATGGTTTTATAATAAGGTGATGTCGAATATGCTTTATTTCTATGGCATGTGTGGTTTGCTAAAAGAGTGTTTGCTTCAGCGATACTTCAGTAAGGAGGTTCGTGGTAGTGTTGAAGTTCCAGAGTCGGATATAGTTCAAGCATGCAGAAAATTGCTGGAAGAGAGGCAGAGCTTAAATGTTTGGAGATTTCTTCATGCAATTAATCGGAGACCTGACATAACCCAAGGGTTAAAATCTCTAAGGTGTCGCACGCTTATATTTGTTGGGGATAGCTCTCCCTTCCATTCGGAGGCTCTCCACATGACCTCAAAGTTGGACAGGAGATATAGTGCCTTAGTAGAGGTCCAGGCTTGTGGATCCATGGTGACAGAGGAGCAGCCACACGCCATGTTGATACCAATGGAGTACTTCTTCATGGGGTATGGCCTGTACCGGCCATGCCATTTCAGCGACAGCCCTAGGAGCCCGCTCAGCCCGTCTTGCATCTCCCCGGAGCTTCTCTCCCCAGAAAGCATGGGCCTGAAACTAAAACCGATAAAGACCCGTGTTTCACTTGGCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ndr

Protein Analysis

348

Amino Acids

38.82

Weight (kDa)

6.07

Isoelectric Point (pI)

51.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ndr PF03096 21 - 305 6.8e-116 Ndr family
Abhydrolase_1 PF00561 63 - 176 2.8e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 489
AccBSI CCGCTC 1 cut(s) 949
AccIII TCCGGA 1 cut(s) 193
AciI CCGC 3 cut(s) 200, 380, 947
AclWI GGATC 5 cut(s) 281, 294, 316, 831, 844
AcoI YGGCCR 2 cut(s) 250, 914
AcuI CTGAAG 4 cut(s) 255, 282, 539, 550
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 2 cut(s) 887, 911
AfiI CCNNNNNNNGG 2 cut(s) 34, 769
AgsI TTSAA 2 cut(s) 593, 617
AjnI CCWGG 2 cut(s) 247, 825
AluBI AGCT 5 cut(s) 152, 266, 651, 753, 976
AluI AGCT 5 cut(s) 152, 266, 651, 753, 976
Alw26I GTCTC 3 cut(s) 28, 105, 682
AlwI GGATC 5 cut(s) 281, 294, 316, 831, 844
AlwNI CAGNNNCTG 1 cut(s) 95
Aor13HI TCCGGA 1 cut(s) 193
AoxI GGCC 5 cut(s) 250, 904, 914, 997, 1036
ApeKI GCWGC 3 cut(s) 211, 266, 856
AseI ATTAAT 1 cut(s) 681
AspA2I CCTAGG 1 cut(s) 938
AspLEI GCGC 1 cut(s) 466
AspS9I GGNCC 2 cut(s) 823, 997
AsuC2I CCSGG 1 cut(s) 971
AsuHPI GGTGA 3 cut(s) 385, 508, 856
AsuII TTCGAA 1 cut(s) 73
AvaII GGWCC 1 cut(s) 823
AvrII CCTAGG 1 cut(s) 938
BaeI ACNNNNGTAYC 2 cut(s) 553, 586
BalI TGGCCA 1 cut(s) 252
BamHI GGATCC 2 cut(s) 286, 836
BanII GRGCYC 1 cut(s) 947
BbvI GCAGC 3 cut(s) 198, 253, 868
BceAI ACGGC 1 cut(s) 5
BciT130I CCWGG 2 cut(s) 249, 827
BciVI GTATCC 1 cut(s) 457
BcnI CCSGG 1 cut(s) 971
BcoDI GTCTC 3 cut(s) 28, 105, 682
BfaI CTAG 1 cut(s) 939
BfoI RGCGCY 1 cut(s) 467
BfuI GTATCC 1 cut(s) 457
BglII AGATCT 1 cut(s) 42
BisI GCNGC 4 cut(s) 201, 212, 267, 857
BlnI CCTAGG 1 cut(s) 938
BlpI GCTNAGC 1 cut(s) 950
BlsI GCNGC 4 cut(s) 202, 213, 268, 858
BmcAI AGTACT 1 cut(s) 887
Bme1390I CCNGG 3 cut(s) 249, 827, 971
Bme18I GGWCC 1 cut(s) 823
BmgT120I GGNCC 2 cut(s) 823, 997
BmiI GGNNCC 3 cut(s) 288, 838, 944
BmrFI CCNGG 3 cut(s) 249, 827, 971
BmsI GCATC 1 cut(s) 972
BplI GAGNNNNNCTC 2 cut(s) 965, 997
Bpu1102I GCTNAGC 1 cut(s) 950
Bpu14I TTCGAA 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 1 cut(s) 971
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 2 cut(s) 105, 682
BsaJI CCNNGG 6 cut(s) 175, 430, 703, 840, 938, 969
BsaWI WCCGGW 1 cut(s) 193
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 793, 823
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 769
Bse118I RCCGGY 1 cut(s) 912
Bse1I ACTGG 1 cut(s) 82
BseAI TCCGGA 1 cut(s) 193
BseBI CCWGG 2 cut(s) 249, 827
BseDI CCNNGG 6 cut(s) 175, 430, 703, 840, 938, 969
BseLI CCNNNNNNNGG 2 cut(s) 34, 769
BseMII CTCAG 1 cut(s) 964
BseNI ACTGG 1 cut(s) 82
BseRI GAGGAG 1 cut(s) 866
BseXI GCAGC 3 cut(s) 198, 253, 868
BsgI GTGCAG 2 cut(s) 111, 375
BshFI GGCC 5 cut(s) 252, 906, 916, 999, 1038
BsiSI CCGG 3 cut(s) 194, 913, 971
BslI CCNNNNNNNGG 2 cut(s) 34, 769
BsmAI GTCTC 3 cut(s) 28, 105, 682
BsmBI CGTCTC 1 cut(s) 28
BsnI GGCC 5 cut(s) 252, 906, 916, 999, 1038
Bso31I GGTCTC 2 cut(s) 105, 682
Bsp119I TTCGAA 1 cut(s) 73
Bsp1286I GDGCHC 1 cut(s) 947
Bsp13I TCCGGA 1 cut(s) 193
Bsp143I GATC 5 cut(s) 42, 286, 316, 321, 836
Bsp1720I GCTNAGC 1 cut(s) 950
Bsp19I CCATGG 1 cut(s) 840
BspACI CCGC 3 cut(s) 200, 380, 947
BspANI GGCC 5 cut(s) 252, 906, 916, 999, 1038
BspCNI CTCAG 1 cut(s) 963
BspEI TCCGGA 1 cut(s) 193
BspLI GGNNCC 3 cut(s) 288, 838, 944
BspPI GGATC 5 cut(s) 281, 294, 316, 831, 844
BspT104I TTCGAA 1 cut(s) 73
BspTNI GGTCTC 2 cut(s) 105, 682
BsrBI CCGCTC 1 cut(s) 949
BsrFI RCCGGY 1 cut(s) 912
BsrI ACTGG 1 cut(s) 82
BssAI RCCGGY 1 cut(s) 912
BssECI CCNNGG 6 cut(s) 175, 430, 703, 840, 938, 969
BssMI GATC 5 cut(s) 42, 286, 316, 321, 836
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 5 cut(s) 175, 430, 703, 840, 938
Bst2UI CCWGG 2 cut(s) 249, 827
Bst6I CTCTTC 1 cut(s) 633
BstACI GRCGYC 1 cut(s) 18
BstBI TTCGAA 1 cut(s) 73
BstC8I GCNNGC 4 cut(s) 126, 622, 731, 947
BstDEI CTNAG 3 cut(s) 719, 814, 950
BstDSI CCRYGG 1 cut(s) 840
BstH2I RGCGCY 1 cut(s) 467
BstHHI GCGC 1 cut(s) 466
BstKTI GATC 5 cut(s) 45, 289, 319, 324, 839
BstMAI GTCTC 3 cut(s) 28, 105, 682
BstMBI GATC 5 cut(s) 42, 286, 316, 321, 836
BstMWI GCNNNNNNNGC 4 cut(s) 927, 942, 951, 960
BstNI CCWGG 2 cut(s) 249, 827
BstNSI RCATGY 2 cut(s) 529, 624
BstSCI CCNGG 3 cut(s) 247, 825, 969
BstV1I GCAGC 3 cut(s) 198, 253, 868
BstX2I RGATCY 4 cut(s) 42, 286, 321, 836
BstXI CCANNNNNNTGG 1 cut(s) 833
BstYI RGATCY 4 cut(s) 42, 286, 321, 836
BsuI GTATCC 1 cut(s) 457
BsuRI GGCC 5 cut(s) 252, 906, 916, 999, 1038
BtgI CCRYGG 1 cut(s) 840
BtsIMutI CAGTG 1 cut(s) 96
Cac8I GCNNGC 4 cut(s) 126, 622, 731, 947
CaiI CAGNNNCTG 1 cut(s) 95
CfoI GCGC 1 cut(s) 466
Cfr10I RCCGGY 1 cut(s) 912
Cfr13I GGNCC 2 cut(s) 823, 997
CseI GACGC 1 cut(s) 26
Csp6I GTAC 2 cut(s) 886, 910
CviQI GTAC 2 cut(s) 886, 910
DdeI CTNAG 3 cut(s) 719, 814, 950
DpnI GATC 5 cut(s) 44, 288, 318, 323, 838
DpnII GATC 5 cut(s) 42, 286, 316, 321, 836
EaeI YGGCCR 2 cut(s) 250, 914
Eam1104I CTCTTC 1 cut(s) 633
EarI CTCTTC 1 cut(s) 633
Eco130I CCWWGG 5 cut(s) 175, 430, 703, 840, 938
Eco24I GRGCYC 1 cut(s) 947
Eco31I GGTCTC 2 cut(s) 105, 682
Eco47I GGWCC 1 cut(s) 823
Eco57I CTGAAG 4 cut(s) 255, 282, 539, 550
EcoRII CCWGG 2 cut(s) 247, 825
EcoT14I CCWWGG 5 cut(s) 175, 430, 703, 840, 938
EcoT38I GRGCYC 1 cut(s) 947
ErhI CCWWGG 5 cut(s) 175, 430, 703, 840, 938
Esp3I CGTCTC 1 cut(s) 28
FauI CCCGC 2 cut(s) 373, 954
Fnu4HI GCNGC 4 cut(s) 201, 212, 267, 857
FriOI GRGCYC 1 cut(s) 947
Fsp4HI GCNGC 4 cut(s) 201, 212, 267, 857
FspBI CTAG 1 cut(s) 939
GlaI GCGC 1 cut(s) 465
GluI GCNGC 4 cut(s) 201, 212, 267, 857
HaeII RGCGCY 1 cut(s) 467
HaeIII GGCC 5 cut(s) 252, 906, 916, 999, 1038
HapII CCGG 3 cut(s) 194, 913, 971
HgaI GACGC 1 cut(s) 26
HhaI GCGC 1 cut(s) 466
Hin1I GRCGYC 1 cut(s) 18
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HinfI GANTC 2 cut(s) 8, 602
HpaII CCGG 3 cut(s) 194, 913, 971
HphI GGTGA 3 cut(s) 385, 508, 856
Hpy188I TCNGA 4 cut(s) 321, 607, 687, 769
Hpy188III TCNNGA 4 cut(s) 143, 194, 281, 599
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 2 cut(s) 306, 769
HpyCH4V TGCA 8 cut(s) 128, 228, 356, 366, 459, 624, 677, 963
HpyF10VI GCNNNNNNNGC 4 cut(s) 927, 942, 951, 960
HpyF3I CTNAG 3 cut(s) 719, 814, 950
Hsp92I GRCGYC 1 cut(s) 18
HspAI GCGC 1 cut(s) 464
Kpn2I TCCGGA 1 cut(s) 193
Kzo9I GATC 5 cut(s) 42, 286, 316, 321, 836
LmnI GCTCC 6 cut(s) 219, 263, 421, 853, 942, 973
Lsp1109I GCAGC 3 cut(s) 198, 253, 868
LweI GCATC 1 cut(s) 972
MaeI CTAG 1 cut(s) 939
MaeIII GTNAC 2 cut(s) 373, 844
MalI GATC 5 cut(s) 44, 288, 318, 323, 838
MbiI CCGCTC 1 cut(s) 949
MboI GATC 5 cut(s) 42, 286, 316, 321, 836
MboII GAAGA 4 cut(s) 52, 650, 662, 883
MflI RGATCY 4 cut(s) 42, 286, 321, 836
MhlI GDGCHC 1 cut(s) 947
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 4 cut(s) 220, 337, 629, 678
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 1 cut(s) 611
MmeI TCCRAC 2 cut(s) 585, 774
Mox20I TGGCCA 1 cut(s) 252
MroI TCCGGA 1 cut(s) 193
MscI TGGCCA 1 cut(s) 252
MseI TTAA 3 cut(s) 653, 681, 710
MslI CAYNNNNRTG 1 cut(s) 371
Msp20I TGGCCA 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 380
MspI CCGG 3 cut(s) 194, 913, 971
MspR9I CCNGG 3 cut(s) 249, 827, 971
MvaI CCWGG 2 cut(s) 249, 827
MwoI GCNNNNNNNGC 4 cut(s) 927, 942, 951, 960
NciI CCSGG 1 cut(s) 971
NcoI CCATGG 1 cut(s) 840
NdeII GATC 5 cut(s) 42, 286, 316, 321, 836
NlaIV GGNNCC 3 cut(s) 288, 838, 944
NmuCI GTSAC 2 cut(s) 373, 844
NspI RCATGY 2 cut(s) 529, 624
NspV TTCGAA 1 cut(s) 73
PaeI GCATGC 1 cut(s) 624
PfeI GAWTC 1 cut(s) 8
PflFI GACNNNGTC 1 cut(s) 20
PkrI GCNGC 4 cut(s) 202, 213, 268, 858
PleI GAGTC 1 cut(s) 610
PpsI GAGTC 1 cut(s) 610
PshBI ATTAAT 1 cut(s) 681
PsiI TTATAA 1 cut(s) 489
Psp6I CCWGG 2 cut(s) 247, 825
PspGI CCWGG 2 cut(s) 247, 825
PspN4I GGNNCC 3 cut(s) 288, 838, 944
PspPI GGNCC 2 cut(s) 823, 997
PstNI CAGNNNCTG 1 cut(s) 95
PsuI RGATCY 4 cut(s) 42, 286, 321, 836
PsyI GACNNNGTC 1 cut(s) 20
RsaI GTAC 2 cut(s) 887, 911
RsaNI GTAC 2 cut(s) 886, 910
RseI CAYNNNNRTG 1 cut(s) 371
SaqAI TTAA 3 cut(s) 653, 681, 710
SatI GCNGC 4 cut(s) 201, 212, 267, 857
Sau3AI GATC 5 cut(s) 42, 286, 316, 321, 836
Sau96I GGNCC 2 cut(s) 823, 997
ScaI AGTACT 1 cut(s) 887
SchI GAGTC 1 cut(s) 611
ScrFI CCNGG 3 cut(s) 249, 827, 971
SduI GDGCHC 1 cut(s) 947
SfaNI GCATC 1 cut(s) 972
SfuI TTCGAA 1 cut(s) 73
SinI GGWCC 1 cut(s) 823
SmiMI CAYNNNNRTG 1 cut(s) 371
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
SphI GCATGC 1 cut(s) 624
Sse9I AATT 4 cut(s) 220, 337, 629, 678
SsiI CCGC 3 cut(s) 200, 380, 947
SspMI CTAG 1 cut(s) 939
StyD4I CCNGG 3 cut(s) 247, 825, 969
StyI CCWWGG 5 cut(s) 175, 430, 703, 840, 938
TaqI TCGA 3 cut(s) 30, 73, 503
TasI AATT 4 cut(s) 220, 337, 629, 678
TatI WGTACW 1 cut(s) 885
TauI GCSGC 1 cut(s) 203
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 3 cut(s) 653, 681, 710
Tru9I TTAA 3 cut(s) 653, 681, 710
TscAI CASTG 1 cut(s) 96
TseFI GTSAC 2 cut(s) 373, 844
TseI GCWGC 3 cut(s) 211, 266, 856
Tsp45I GTSAC 2 cut(s) 373, 844
TspDTI ATGAA 3 cut(s) 425, 662, 883
TspRI CASTG 1 cut(s) 96
Tth111I GACNNNGTC 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 823
VspI ATTAAT 1 cut(s) 681
XceI RCATGY 2 cut(s) 529, 624
XmaJI CCTAGG 1 cut(s) 938
XspI CTAG 1 cut(s) 939
ZrmI AGTACT 1 cut(s) 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.