pycom15g22990

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0003343
Physical Location & Seq
Reverse (-)
17073869 .. 17078506
4638 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1971 bp
ATGGGAAGGGAACCAGAGCAGTTACCCGACCTGAATGACACTCAGTATAACAAGTTGGATGAGCTCCTAACACAAACTCAGCTTTACTCACAGTTTTTGCTGGAGAAAATGGATAACATCACACTTATTGGAGCCAATCAACCGAGTGAAACTGTTGAGGAAAAGAATGTTGAGGAGAAGAAAGGTCGTGGTAGGAAAAGAAAAGCAGCTGCAAATTTTGATAATAGGAAGGCCAAGAAGGCAGTTGAAGCTATGCTTACAAGATCTAAAGAGGGTGTGAAAACTGAAGATGAGAACCTCACTGAAGAAGAAAGAATTGAGAAAGAGCAAAGGGAACTTGTACCTCTGTTGACTGGTGGAAAATTGAAGTCTTATCAAATCAAAGGTGTAAAGTGGTTGATCTCATTATGGCAAAATGGGCTGAATGGGATCCTTGCAGATCAAATGGGACTTGGAAAAACCATCCAGACCATTGGTTTTCTTGCTCATCTAAAAGGGAATGGAATGGATGGGCCCTACTTAGTGATTGCTCCTCTTTCTACTCTTGCGAATTGGGTAAATGAAATTTCAAGGTTCACGCCTTCAATAAATGCTATTGTCTACCATGGTGACAAGAAACAAAGGGATGAGATACGAAGGAAGCACATGCCTCGACAAATTGGCCCTAAGTTCCCTATAATTGTTACTTCATATGAAGTTGCAATGGCTGATGCAAGAAAATGTTTGAGACATTACAATTGGAAATATCTTGTGGTTGATGAAGGACACAGATTGAAGAACTCAAAGTGCAAACTACTGCGGGAATTGAAATTATTACATGTAGACAATAAGATTCTGTTGACTGGGACACCTCTACAGAATAATCTGGCAGAGCTTTGGTCATTATTGAATTTCATTTTGCCTGATATATTCTCATCCCATGAAGAATTTGAGTCATGGTTTGATTTAGCAGGAAAGTGCAGTAATGAGGCAATGAAGGAAGAATTGGAAGAGAAGAGAAGGGCTCAAATGGTTGCAAAACTCCATGCCATCTTGCGTCCTTTTCTTCTTCGAAGAATGAAGTCTGATGTTGAGCACATGCTTCCTAGGAAAAAGGAAATCATATTATATGCATCCATGACAGATCATCAAAAACATTTTCAAGATCTTCTGATCAATAAGACATTGGAGAATTATTTACTTGAGAGGGGAGATCATGTTCGCGGTATGAAAGTAAAGCTTAACAATCTGATGGTCCAACTTCGGAAGAACTGCAATCATCCTGACCTTTTGGAGTCGGCATTTGATGGCTCATATTACTACCCACCTGTTGAACAGATTGTTGAACAGTGTGGAAAATTTAGTTTGCTGGACCGACTGTTAAAGCACCTGTTTGCCCGCAAACACAAAGTTCTTATCTTCTCTCAGTGGACTAAGATTTTGGATATTATGGAGTACTATTTTAGTGAGAAAGGATTTGAAGTTTGTAGAATTGATGGCAGTGTGAAACTTGAAGAACGGAGAAGTCAGATTGCAGCGTTCAACGAAGTGGACAGCAATTATAGAATTTTTCTTCTAAGCACCAGAGCTGGTGGACTGGGTATTAACCTTACTGCAGCTGACACTTGTATACTGTACGACAGTGATTGGAACCCTCAAATGGATCTGCAAGCCATGGATAGATGCCACAGGATTGGTCAAACAAAGCCTGTTCATGTTTACAGGTTGGCGACGGCACAATCTGTAGAGGGTCGGATTCTGAAAAGAGCTTTTAGCAAGTTGAAGCTTGAACATGTGGTGATTGGGAAGGGGCAGTTTCATCAAGATAAAGCGAAGAGTAGCACTGATGTCTTGGAGGAAGAGGATCTTATAGCTTTGCTAAGAGAGGAAGATTCTGCTGAAGACAAGATGATACAGACAGTTATCAGTGACAAAGATTTGGAGAGGGTGTTAGATCGCAGTGATCTAGTCTGCAGTCCTGCTCGATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000785 GO:0000786 GO:0003674 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005694 GO:0006139 GO:0006259 GO:0006325 GO:0006342 GO:0006344 GO:0006346 GO:0006349 GO:0006355 GO:0006725 GO:0006807 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009292 GO:0009294 GO:0009889 GO:0009890 GO:0009892 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010604 GO:0010605 GO:0010629 GO:0010638 GO:0010639 GO:0016043 GO:0016458 GO:0016462 GO:0016569 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0031056 GO:0031057 GO:0031058 GO:0031060 GO:0031062 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031399 GO:0031400 GO:0031401 GO:0032196 GO:0032197 GO:0032268 GO:0032269 GO:0032270 GO:0032392 GO:0032508 GO:0032991 GO:0032993 GO:0033043 GO:0033044 GO:0034641 GO:0035065 GO:0035067 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043412 GO:0044030 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044446 GO:0044464 GO:0044764 GO:0044815 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051052 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051246 GO:0051247 GO:0051248 GO:0051252 GO:0051253 GO:0051276 GO:0051570 GO:0051574 GO:0051704 GO:0060255 GO:0065007 GO:0070035 GO:0071103 GO:0071514 GO:0071704 GO:0071840 GO:0080090 GO:0090239 GO:0090241 GO:0090304 GO:0140097 GO:1901360 GO:1901983 GO:1901984 GO:1902275 GO:1902679 GO:1903506 GO:1903507 GO:1905268 GO:1905269 GO:2000112 GO:2000113 GO:2000756 GO:2000757 GO:2001141 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Helicase_C SNF2_N

Protein Analysis

657

Amino Acids

75.72

Weight (kDa)

8.56

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 121 - 284 3.1e-09 Type III restriction enzyme, res subunit
SNF2-rel_dom PF00176 125 - 423 3.5e-85 SNF2-related domain
DEAD PF00270 139 - 287 2.5e-07 DEAD/DEAH box helicase
HDA2-3 PF11496 371 - 581 6.5e-06 Class II histone deacetylase complex subunits 2 and 3
Helicase_C PF00271 446 - 559 5.3e-27 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 600, 822, 1609
AccII CGCG 1 cut(s) 1203
AciI CCGC 3 cut(s) 799, 1203, 1378
AclWI GGATC 4 cut(s) 424, 437, 1650, 1851
AcsI RAATTY 6 cut(s) 214, 564, 889, 926, 1337, 1545
AcuI CTGAAG 3 cut(s) 306, 324, 1899
AfaI GTAC 3 cut(s) 342, 1436, 1616
AfiI CCNNNNNNNGG 1 cut(s) 1639
AflIII ACRYGT 2 cut(s) 817, 1771
AjuI GAANNNNNNNTTGG 2 cut(s) 968, 1000
AloI GAACNNNNNNTCC 2 cut(s) 1182, 1214
Alw21I GWGCWC 2 cut(s) 66, 1077
Alw26I GTCTC 1 cut(s) 721
AlwI GGATC 4 cut(s) 424, 437, 1650, 1851
AoxI GGCC 3 cut(s) 231, 512, 661
ApaI GGGCCC 1 cut(s) 516
ApeKI GCWGC 4 cut(s) 206, 209, 1514, 1595
ApoI RAATTY 6 cut(s) 214, 564, 889, 926, 1337, 1545
ArsI GACNNNNNNTTYG 2 cut(s) 1402, 1434
AspA2I CCTAGG 1 cut(s) 1085
AspS9I GGNCC 5 cut(s) 512, 513, 662, 1234, 1351
AsuHPI GGTGA 2 cut(s) 620, 1789
AsuII TTCGAA 1 cut(s) 1051
AvaII GGWCC 2 cut(s) 1234, 1351
AvrII CCTAGG 1 cut(s) 1085
BaeGI GKGCMC 1 cut(s) 516
BamHI GGATCC 1 cut(s) 429
BanII GRGCYC 3 cut(s) 66, 516, 1006
BbsI GAAGAC 1 cut(s) 1887
Bbv12I GWGCWC 2 cut(s) 66, 1077
BbvI GCAGC 4 cut(s) 196, 218, 1526, 1607
BccI CCATC 6 cut(s) 470, 503, 1037, 1225, 1280, 1469
BceAI ACGGC 1 cut(s) 1728
BcgI CGANNNNNNTGC 2 cut(s) 632, 666
BclI TGATCA 1 cut(s) 1152
BcoDI GTCTC 1 cut(s) 721
BfaI CTAG 2 cut(s) 1086, 1946
BfmI CTRYAG 4 cut(s) 854, 1593, 1722, 1951
BglI GCCNNNNNGGC 1 cut(s) 239
BglII AGATCT 2 cut(s) 263, 1144
BisI GCNGC 4 cut(s) 207, 210, 1515, 1596
BlnI CCTAGG 1 cut(s) 1085
BlsI GCNGC 4 cut(s) 208, 211, 1516, 1597
BmcAI AGTACT 1 cut(s) 1436
Bme18I GGWCC 2 cut(s) 1234, 1351
BmgT120I GGNCC 5 cut(s) 512, 513, 662, 1234, 1351
BmiI GGNNCC 5 cut(s) 12, 133, 431, 514, 1631
BmrI ACTGGG 2 cut(s) 852, 1586
BmsI GCATC 3 cut(s) 700, 1121, 1652
BmuI ACTGGG 2 cut(s) 852, 1586
BpiI GAAGAC 1 cut(s) 1887
BplI GAGNNNNNCTC 2 cut(s) 988, 1020
BpmI CTGGAG 1 cut(s) 122
Bpu14I TTCGAA 1 cut(s) 1051
BpuEI CTTGAG 1 cut(s) 1202
BsaJI CCNNGG 3 cut(s) 604, 1085, 1653
BsaXI ACNNNNNCTCC 4 cut(s) 1182, 1212, 1913, 1943
Bsc4I CCNNNNNNNGG 1 cut(s) 1639
Bse1I ACTGG 3 cut(s) 358, 847, 1581
Bse3DI GCAATG 2 cut(s) 708, 978
BseDI CCNNGG 3 cut(s) 604, 1085, 1653
BseGI GGATG 7 cut(s) 64, 462, 514, 631, 914, 1112, 1258
BseLI CCNNNNNNNGG 1 cut(s) 1639
BseMI GCAATG 2 cut(s) 708, 978
BseMII CTCAG 3 cut(s) 56, 92, 1418
BseNI ACTGG 3 cut(s) 358, 847, 1581
BseRI GAGGAG 2 cut(s) 188, 522
BseSI GKGCMC 1 cut(s) 516
BseXI GCAGC 4 cut(s) 196, 218, 1526, 1607
BsgI GTGCAG 1 cut(s) 979
Bsh1236I CGCG 1 cut(s) 1203
BshFI GGCC 3 cut(s) 233, 514, 663
BsiHKAI GWGCWC 2 cut(s) 66, 1077
BslFI GGGAC 2 cut(s) 462, 859
BslI CCNNNNNNNGG 1 cut(s) 1639
BsmAI GTCTC 1 cut(s) 721
BsmFI GGGAC 2 cut(s) 462, 859
BsnI GGCC 3 cut(s) 233, 514, 663
Bsp119I TTCGAA 1 cut(s) 1051
Bsp120I GGGCCC 1 cut(s) 512
Bsp1286I GDGCHC 4 cut(s) 66, 516, 1006, 1077
Bsp19I CCATGG 2 cut(s) 604, 1653
BspACI CCGC 3 cut(s) 799, 1203, 1378
BspANI GGCC 3 cut(s) 233, 514, 663
BspCNI CTCAG 3 cut(s) 55, 91, 1417
BspFNI CGCG 1 cut(s) 1203
BspHI TCATGA 1 cut(s) 1967
BspLI GGNNCC 5 cut(s) 12, 133, 431, 514, 1631
BspMAI CTGCAG 2 cut(s) 1597, 1955
BspPI GGATC 4 cut(s) 424, 437, 1650, 1851
BspT104I TTCGAA 1 cut(s) 1051
BsrDI GCAATG 2 cut(s) 708, 978
BsrI ACTGG 3 cut(s) 358, 847, 1581
BssECI CCNNGG 3 cut(s) 604, 1085, 1653
BssNAI GTATAC 1 cut(s) 1610
BssT1I CCWWGG 3 cut(s) 604, 1085, 1653
Bst1107I GTATAC 1 cut(s) 1610
Bst4CI ACNGT 7 cut(s) 93, 154, 1329, 1359, 1614, 1622, 1900
Bst6I CTCTTC 4 cut(s) 984, 989, 1808, 1833
BstBI TTCGAA 1 cut(s) 1051
BstC8I GCNNGC 2 cut(s) 1378, 1650
BstDEI CTNAG 8 cut(s) 42, 78, 520, 666, 1404, 1413, 1556, 1859
BstDSI CCRYGG 2 cut(s) 604, 1653
BstF5I GGATG 7 cut(s) 64, 462, 514, 631, 914, 1112, 1258
BstFNI CGCG 1 cut(s) 1203
BstMAI GTCTC 1 cut(s) 721
BstMWI GCNNNNNNNGC 3 cut(s) 239, 248, 418
BstNSI RCATGY 4 cut(s) 649, 821, 1081, 1775
BstSFI CTRYAG 4 cut(s) 854, 1593, 1722, 1951
BstSLI GKGCMC 1 cut(s) 516
BstUI CGCG 1 cut(s) 1203
BstV1I GCAGC 4 cut(s) 196, 218, 1526, 1607
BstV2I GAAGAC 1 cut(s) 1887
BstX2I RGATCY 5 cut(s) 263, 429, 1144, 1642, 1843
BstXI CCANNNNNNTGG 2 cut(s) 473, 1673
BstYI RGATCY 5 cut(s) 263, 429, 1144, 1642, 1843
BstZ17I GTATAC 1 cut(s) 1610
BsuRI GGCC 3 cut(s) 233, 514, 663
BtgI CCRYGG 2 cut(s) 604, 1653
BtsCI GGATG 7 cut(s) 64, 462, 514, 631, 914, 1112, 1258
BtsI GCAGTG 2 cut(s) 1486, 1945
BtsIMutI CAGTG 8 cut(s) 300, 1334, 1412, 1486, 1627, 1821, 1912, 1945
Cac8I GCNNGC 2 cut(s) 1378, 1650
CciI TCATGA 1 cut(s) 1967
Cfr13I GGNCC 5 cut(s) 512, 513, 662, 1234, 1351
CseI GACGC 1 cut(s) 1025
Csp6I GTAC 3 cut(s) 341, 1435, 1615
CviQI GTAC 3 cut(s) 341, 1435, 1615
DdeI CTNAG 8 cut(s) 42, 78, 520, 666, 1404, 1413, 1556, 1859
Eam1104I CTCTTC 4 cut(s) 984, 989, 1808, 1833
EarI CTCTTC 4 cut(s) 984, 989, 1808, 1833
Ecl136II GAGCTC 1 cut(s) 64
Eco130I CCWWGG 3 cut(s) 604, 1085, 1653
Eco24I GRGCYC 3 cut(s) 66, 516, 1006
Eco47I GGWCC 2 cut(s) 1234, 1351
Eco53kI GAGCTC 1 cut(s) 64
Eco57I CTGAAG 3 cut(s) 306, 324, 1899
EcoICRI GAGCTC 1 cut(s) 64
EcoO109I RGGNCCY 1 cut(s) 513
EcoT14I CCWWGG 3 cut(s) 604, 1085, 1653
EcoT22I ATGCAT 1 cut(s) 1114
EcoT38I GRGCYC 3 cut(s) 66, 516, 1006
ErhI CCWWGG 3 cut(s) 604, 1085, 1653
FalI AAGNNNNNCTT 6 cut(s) 240, 272, 1203, 1235, 1830, 1862
FaqI GGGAC 2 cut(s) 462, 859
FauI CCCGC 2 cut(s) 792, 1385
FauNDI CATATG 1 cut(s) 691
FbaI TGATCA 1 cut(s) 1152
FblI GTMKAC 3 cut(s) 600, 822, 1609
Fnu4HI GCNGC 4 cut(s) 207, 210, 1515, 1596
FokI GGATG 7 cut(s) 71, 449, 521, 638, 901, 1099, 1245
FriOI GRGCYC 3 cut(s) 66, 516, 1006
Fsp4HI GCNGC 4 cut(s) 207, 210, 1515, 1596
FspBI CTAG 2 cut(s) 1086, 1946
GluI GCNGC 4 cut(s) 207, 210, 1515, 1596
GsuI CTGGAG 1 cut(s) 122
HaeIII GGCC 3 cut(s) 233, 514, 663
HgaI GACGC 1 cut(s) 1025
HincII GTYRAC 2 cut(s) 351, 840
HindII GTYRAC 2 cut(s) 351, 840
HindIII AAGCTT 2 cut(s) 1217, 1763
HinfI GANTC 5 cut(s) 832, 932, 1274, 1735, 1871
HphI GGTGA 2 cut(s) 620, 1789
Hpy188I TCNGA 7 cut(s) 1066, 1152, 1230, 1245, 1509, 1734, 1740
Hpy188III TCNNGA 5 cut(s) 466, 1142, 1262, 1802, 1968
Hpy99I CGWCG 1 cut(s) 1714
HpyAV CCTTC 8 cut(s) 223, 232, 591, 630, 755, 970, 993, 1780
HpyCH4III ACNGT 7 cut(s) 93, 154, 1329, 1359, 1614, 1622, 1900
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 248, 418
HpyF3I CTNAG 8 cut(s) 42, 78, 520, 666, 1404, 1413, 1556, 1859
Ksp22I TGATCA 1 cut(s) 1152
LmnI GCTCC 3 cut(s) 69, 131, 535
Lsp1109I GCAGC 4 cut(s) 196, 218, 1526, 1607
LweI GCATC 3 cut(s) 700, 1121, 1652
MaeI CTAG 2 cut(s) 1086, 1946
MaeIII GTNAC 4 cut(s) 21, 608, 682, 1907
MfeI CAATTG 1 cut(s) 736
MflI RGATCY 5 cut(s) 263, 429, 1144, 1642, 1843
MhlI GDGCHC 4 cut(s) 66, 516, 1006, 1077
MlyI GAGTC 2 cut(s) 941, 1283
MmeI TCCRAC 3 cut(s) 36, 1261, 1712
Mph1103I ATGCAT 1 cut(s) 1114
MseI TTAA 3 cut(s) 1221, 1361, 1584
MspA1I CMGCKG 2 cut(s) 209, 1598
MunI CAATTG 1 cut(s) 736
MvnI CGCG 1 cut(s) 1203
MwoI GCNNNNNNNGC 3 cut(s) 239, 248, 418
NcoI CCATGG 2 cut(s) 604, 1653
NdeI CATATG 1 cut(s) 691
NlaIV GGNNCC 5 cut(s) 12, 133, 431, 514, 1631
NmuCI GTSAC 2 cut(s) 608, 1907
NsiI ATGCAT 1 cut(s) 1114
NspI RCATGY 4 cut(s) 649, 821, 1081, 1775
NspV TTCGAA 1 cut(s) 1051
PagI TCATGA 1 cut(s) 1967
PciI ACATGT 2 cut(s) 817, 1771
PfeI GAWTC 3 cut(s) 832, 1735, 1871
PkrI GCNGC 4 cut(s) 208, 211, 1516, 1597
PleI GAGTC 2 cut(s) 940, 1282
PpsI GAGTC 2 cut(s) 940, 1282
PscI ACATGT 2 cut(s) 817, 1771
Psp124BI GAGCTC 1 cut(s) 66
PspN4I GGNNCC 5 cut(s) 12, 133, 431, 514, 1631
PspOMI GGGCCC 1 cut(s) 512
PspPI GGNCC 5 cut(s) 512, 513, 662, 1234, 1351
PstI CTGCAG 2 cut(s) 1597, 1955
PsuI RGATCY 5 cut(s) 263, 429, 1144, 1642, 1843
PvuII CAGCTG 2 cut(s) 209, 1598
RsaI GTAC 3 cut(s) 342, 1436, 1616
RsaNI GTAC 3 cut(s) 341, 1435, 1615
SacI GAGCTC 1 cut(s) 66
SaqAI TTAA 3 cut(s) 1221, 1361, 1584
SatI GCNGC 4 cut(s) 207, 210, 1515, 1596
Sau96I GGNCC 5 cut(s) 512, 513, 662, 1234, 1351
ScaI AGTACT 1 cut(s) 1436
SchI GAGTC 2 cut(s) 941, 1283
SduI GDGCHC 4 cut(s) 66, 516, 1006, 1077
SfaNI GCATC 3 cut(s) 700, 1121, 1652
SfcI CTRYAG 4 cut(s) 854, 1593, 1722, 1951
SfuI TTCGAA 1 cut(s) 1051
SinI GGWCC 2 cut(s) 1234, 1351
SmlI CTYRAG 1 cut(s) 1181
SmoI CTYRAG 1 cut(s) 1181
SsiI CCGC 3 cut(s) 799, 1203, 1378
SspMI CTAG 2 cut(s) 1086, 1946
SstI GAGCTC 1 cut(s) 66
StyI CCWWGG 3 cut(s) 604, 1085, 1653
TaaI ACNGT 7 cut(s) 93, 154, 1329, 1359, 1614, 1622, 1900
TaqI TCGA 3 cut(s) 652, 1051, 1963
TaqII GACCGA 1 cut(s) 1368
TatI WGTACW 1 cut(s) 1434
TfiI GAWTC 3 cut(s) 832, 1735, 1871
Tru1I TTAA 3 cut(s) 1221, 1361, 1584
Tru9I TTAA 3 cut(s) 1221, 1361, 1584
TscAI CASTG 8 cut(s) 307, 1334, 1412, 1486, 1627, 1828, 1912, 1945
TseFI GTSAC 2 cut(s) 608, 1907
TseI GCWGC 4 cut(s) 206, 209, 1514, 1595
Tsp45I GTSAC 2 cut(s) 608, 1907
TspGWI ACGGA 1 cut(s) 1513
TspRI CASTG 8 cut(s) 307, 1334, 1412, 1486, 1627, 1828, 1912, 1945
VpaK11BI GGWCC 2 cut(s) 1234, 1351
XapI RAATTY 6 cut(s) 214, 564, 889, 926, 1337, 1545
XceI RCATGY 4 cut(s) 649, 821, 1081, 1775
XmaJI CCTAGG 1 cut(s) 1085
XmiI GTMKAC 3 cut(s) 600, 822, 1609
XspI CTAG 2 cut(s) 1086, 1946
ZrmI AGTACT 1 cut(s) 1436
Zsp2I ATGCAT 1 cut(s) 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.