Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0001986

Physical Location & Seq

Genomic Coordinates Reverse (-)

31784312 .. 31792757

Gene Structure 8446 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4209 bp

ATGTCGGAAGCCCAGAGGAAATTGTTGACAGTACCCAGTTTCCATCGTGGAAATGGGTACGTAAATGGGTTACTCAGGCATCCTTCTATGGCATCTAAAGTTGATGAATTCTGCTATGCCCTTGGTGGGAAGAAGCCAATCCATAGCATTTTAATTGCAAACAATGGAATGGCCGCGGTCAAGTTTATTCGTAGTGTGAGGACATGGTCTTATGAGACATTTGGTACAGAGAAGGCTGTTTTATTGGTGGCAATGGCCACACCAGAGGACATGAGAATCAATGCAGAACACATCAGAATCGCCGATCAGTTTGTGGAGGTTCCTGGTGGAACTAACAATAATAATTATGCCAATGTGCAACTCATTATAGAGATGGCAGAGAGAACACATGTTAATGCAGTTTGGCCTGGTTGGGGTCATGCCTCTGAGAACCCTGAGCTTCCAGATGCATTGAATGCAAAGGGAATTGTATTTCTTGGGCCACCATCTATATCTATGGCAGCATTAGGAGATAAAATTGGTTCATCATTAATTGCTCAGGCTGCCAACGTACCTACTCTTCCATGGAGCGGATCACATGTGAAAATTCCTTCAGATAGCTGCTTGGTTACAATCCCAGAGGAAGTATATAGGGAAGCGTGTGTTTATACAACAGAGGAAGCTGTTGCAAGTTGTCAGGTTGTAGGTTACCCAGCAATGATTAAGGCTTCTTGGGGTGGTGGTGGTAAAGGCATAAGAAAGGTCCATAATGATGATGAGGTAAGGGCATTGTTCAAGCAAGTTCAGGGTGAAGTTCCTGGATCTCCAATATTTATAATGAAACTCGCATCCCAGAGCCGGCATTTAGAGGTACAGTTACTGTGTGATCAGTATGGAAATGTTGCAGCTTTGCATAGCCGTGACTGCAGTGTTCAAAGGCGGCACCAGAAGATCATTGAGGAGGGTCCAATTACTGTAGCTCCTATAGAGACTGTAAAAAAGTTGGAGCAATCAGCTCGAAGGTTGGCTAAATCTGTTAATTATGTTGGAGCAGCTACTGTTGAGTACCTCTACAGTATGGAAACTGGCGAGTACTATTTCTTGGAGCTGAACCCTCGATTACAGGTGGAGCACCCAGTCACTGAGTGGATAGCTGAAATGAATCTTCCAGCAGCCCAAGTTGTAGTTGGGATGGGCATACCTCTCTGGCAAATCCCTGAGATAAGGCGATTTTATGGAATGGAACATGGTGGTGGGTATGATGCTTGGAGAAAGACCTCAGCTATTGCTACCCCATTCGATTTTGACAAGGCAGAATCTACAAGGCCAAAGGGTCATTGTGTCGCAGTGCGTGTAACAAGTGAGGATCCAGATGATGGCTTTAAGCCTACCAGTGGGAAAGTACAGGAGTTGAGTTTTAAAAGCAAGCCAAATGTTTGGGCATACTTTTCTGTTAAGTCTGGAGGGGGCATTCATGAATTCTCAGACTCTCAGTTTGGACATGTTTTTGCATTTGGAGAGTCTCGAGCTCTAGCTATTGCAAATATGGTTCTTGGGCTGAAGGAAATTCAAATTAGAGGAGAAGTTCGTACAAATGTTGATTATTCAATAGATCTTCTACATGCCTCAGACTATAGAGAGAATAAAATCCACACGGGTTGGTTGGATAGTAGAATTGCCATGCGTGTTAGAGCAGAAAGGCCTCCTTGGTATCTTTCTGTAGTTGGAGGGACTCTGTTTAAAGCATCTGCCAGTAGTGCAGCAATGGTGTCAGATTATGTGGGTTATCTTGAGAAAGGGCAAATTCCACCCAAGCACATATCACATGTCCATGCACAAGTATCGTTGAACATTGAAGGGAGCAAATACACGATTGACATGGTGAGGGGGGGTCCAGGAAGCTACAGATTGAGGATGAATGAGTCAGTGATTGAAGCAGAGATACATACTTTACGCGATGGGGGTTTGTTGATGCAGTTGGATGGGAACAGTCACATTATATATGCAGAGGAAGAAGCAGCTGGAACTCGCCTTCTTATTGATGGAAGGACTTGCTTGCTTCAGAATGACCATGATCCGTCCAAGTTAATAGCAGAGACACCATGCAAGCTGCTGAGGTACTTGGTTACTGATGGTAGTCATGTTGAAGGTGACACACCATATGCCGAGGTTGAGGTTATGAAGATGTGTATGCCTCTTCTTTCACCTGCTTCTGGAGCTATCCACTTTAGAATGTCTGAAGGTCAAGCAATGCAGGCTGGTGAACTTATTGCAAAGCTTGATTTAGATGATCCTTCGGCTGTAAGAAAGGCAGAACCTTTCCATGGAAGCTTCCCAGTTCTGGGGCCTCCAACTGCAATTTCTGGTAAAGTTCACCAGAGATGTGCTGCTAGCATAATTGCAGCCCGAATGATTCTTGCTGGTTATGAGCATAACATTGATGAAGTAGTGCAAAACTTGCTCACTTGCCTAGACAGTCCTGAACTCCCTTTCCTTCAGTGGCAAGAGTGCTTTGCTGTTCTAGCAACCCGCCTACCCAAAGATCTTAAAAATGAATTGGAATCAAAATTTAAGGAGTTTGAGGTGATTTCTAGTTCTCAGAATGTTGAGTTCCCCGCTAAGTTGTTGCGCAGTGTTCTTGAGGCCCATCTATTCTCCTGTCCTGATAAAGAGAAAGGAGCCCAAGAAAGGCTTGTTGAGCCTTTGATGAGCCTTGTGAAGTCTTATGAGGGTGGACGAGAAAGTCATGCCCGAGTTATCGTCCAATCCCTTTTTGAAGAGTACCTGTCTGTTGAAGAATTATTCAGTGACAACATTCAGGCTGATGTGATTGAACGCCTTCGACTTCAATATAAAAAAGATCTATTGAAGATTGTGGACATTGTGCTTTCTCACCAGGGTGTCAAGAACAAAAATAAGTTGATACTACGACTAATGGAACAACTGGTCTACCCCAACCCTGCTGCGTACAGGGAAAAGCTAATTCGGTTTTCTCAACTTAACCATACAAGCTATTCTGAGTTGGCACTAAAGGCAAGTCAACTGATGGAGCAAACCAAGTTGAGTGAACTTCGTTCCAGCATTGCCAGAAGTCTTTCTGAGTTAGAGATGTTTACCGAGGATGGTGAAAGCATGGATACTCCCAAGAGGAAAAGTGCCATAAATGAACGAATGGAGGATCTTGTGAGTGCTCCTTTGGCAGTTGAAGATGCCCTTGTAGGTCTATTTGATCACAGTGATCACACACTTCAGAGGCGGGTTGTGGAGAGCTATGTGCGTCGGTTATACCAGCCGTATCTTGTAAAGGGGAGTGTGAGGATGCAGTGGCAAAGATCTGGTCTTATGGCTTCCTGGGAGTTCTTGGACGAACATACAGAGAGAAAAAATGCAAATGAAGATCAATCTTCTGGTAAATCAATTGAGAAATACAGTGAGAGAAAATGGGGAGTCATGATTATTATCAAATCCCTTCAATTTCTGCCAGCAATTATTAGTGCTGCATTGAAGGAAATGTCTCATCAACTTCATGAATCAATTCCGAATGGATCTAGTGATCCAAGCGGCTTTGGAAATATGATGCATATTGCTTTAGTTGGCATCAACAATCCGATGAGTTTACTTCAGGATAGTGGCGATGAGGATCAGGCTCAAGAAAGAATTAAGAAGTTAGCGAAAATACTAAAAGAGCAAGGAGTAGCCTCCAGTCTGCACAGTGCAGGTGTCAGGGTAATTAGTTGCATCATACAAAGAGATGAAGGGCGAGCTCCTATGAGGCATTCCTTCCACTGGTCGTCAGAGAAACTCTATTACGAGGAAGAGCCATTATTGCGTCATCTGGAACCACCTCTATCCATTTACCTTGAGTTGGACAAGCTTAAAGGTTATGAGAATGTACAGTATACCCCATCCCGGGACCGTCAGTGGCACTTGTACAGCGTTGTAGATAAGCCACAACCAACCAAAAGGATGTTCCTCAGAACACTTGTAAGGCAGCCCACTTCAAATGATGGGTTCGCTGGATTTCAAAGGCTAGACATGGAAGCAGCTAATAAGCAATGGGCTTTGTCATTTACTTCAAGGAGTATTTTGAGGTCCTTATTGACAGCAATGGAAGAGTTGGAACTTAATGCACACAATGCCAGTGTCAAATCTGACCATACTCATATGTACCTTTATATCTTGCGTGAGCAACAAATTGATCGATCTACTGCCGTACCCCAAGAGAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0003989 GO:0005575 GO:0005623 GO:0005886 GO:0006082 GO:0006629 GO:0006631 GO:0006633 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009653 GO:0009719 GO:0009725 GO:0009735 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009933 GO:0009987 GO:0010014 GO:0010016 GO:0010033 GO:0010072 GO:0010154 GO:0016020 GO:0016053 GO:0016421 GO:0016874 GO:0016885 GO:0019752 GO:0022414 GO:0022622 GO:0030497 GO:0032501 GO:0032502 GO:0032787 GO:0042221 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044464 GO:0046394 GO:0048316 GO:0048364 GO:0048367 GO:0048507 GO:0048508 GO:0048532 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071704 GO:0071944 GO:0072330 GO:0090421 GO:0099402 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00061 ko00254 ko00620 ko00640 ko01100 ko01110 ko01212 ko04152 ko04910 ko04922 map00061 map00254 map00620 map00640 map01100 map01110 map01212 map04152 map04910 map04922

Pfam Domains

Protein Families

ACC_central Biotin_carb_C Biotin_carb_N Biotin_lipoyl CPSase_L_D2 Carboxyl_trans

Protein Analysis

1403

Amino Acids

156.99

Weight (kDa)

6.2

Isoelectric Point (pI)

44.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Biotin_carb_N	PF00289	47 - 165	1.3e-29	Biotin carboxylase, N-terminal domain
CPSase_L_D2	PF02786	214 - 396	5e-45	Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp	PF02222	215 - 366	1.3e-06	ATP-grasp domain
ATP-grasp_3	PF02655	222 - 368	8.6e-08	ATP-grasp domain
Biotin_carb_C	PF02785	443 - 549	1e-23	Biotin carboxylase C-terminal domain
ACCA_BT	PF21385	565 - 680	1.6e-29	Acetyl-CoA carboxylase, BT domain
Biotin_lipoyl	PF00364	690 - 753	3.8e-10	Biotin-requiring enzyme
ACC_central	PF08326	754 - 1394	8e-158	Acetyl-CoA carboxylase, central region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001986)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G36160 AT1G36160 AT1G36180 AT1G36180 AT1G36180
fragaria_vesca	FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000 FvH4_5g38000
malus_domestica	MD08G1178000.v1.1 MD15G1363500.v1.1
prunus_persica	Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1 Prupe.1G512000_v2.0.a1
pyrus_communis	pycom08g15250 pycom15g32670
rosa_chinensis	RchiOBHm_Chr2g0140771 RchiOBHm_Chr2g0144971 RchiOBHm_Chr7g0239931
rosa_laevigata	RLG00000000716 RLG00000013564 RLG00000020589
rosa_multiflora	Rmu_co8457723.1_g000001 Rmu_sc0000540.1_g000024 Rmu_sc0001684.1_g000001 Rmu_sc0006326.1_g000027 Rmu_sc0041871.1_g000001 Rmu_sc0041872.1_g000001
rosa_roxburghii	Rroxscaffold_3G00220610
rosa_rugosa	Rorug03G0235100 Rorug07G0331300 Rorug07G0331300
rosa_samantha	Rh1CG081500 Rh1DG087900 Rh4AG110300 Rh4DG033300 Rh5BG555900 Rh5CG578700 Rh5DG265800 Rh7AG485000 Rh7BG458000 Rh7DG470600
rosa_wichuraiana	Rw7G040680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	815
AarI	CACCTGC	2 cut(s)	2194, 3686
AasI	GACNNNNNNGTC	1 cut(s)	2721
Acc16I	TGCGCA	1 cut(s)	2609
Acc36I	ACCTGC	2 cut(s)	2194, 3686
AccB1I	GGYRCC	1 cut(s)	921
AccB7I	CCANNNNNTGG	1 cut(s)	1355
AccBSI	CCGCTC	1 cut(s)	570
AccI	GTMKAC	2 cut(s)	2928, 3878
AccII	CGCG	2 cut(s)	176, 1935
AciI	CCGC	8 cut(s)	174, 176, 570, 919, 2509, 2595, 3235, 3540
AcoI	YGGCCR	2 cut(s)	171, 255
AcsI	RAATTY	6 cut(s)	107, 585, 1457, 1545, 1782, 2546
AcuI	CTGAAG	7 cut(s)	576, 1559, 2024, 2238, 2459, 3212, 3584
AdeI	CACNNNGTG	1 cut(s)	1125
AflIII	ACRYGT	4 cut(s)	388, 577, 1480, 1804
AjnI	CCWGG	6 cut(s)	322, 406, 796, 1873, 2874, 3329
AjuI	GAANNNNNNNTTGG	4 cut(s)	2323, 2355, 3932, 3964
AleI	CACNNNNGTG	1 cut(s)	2877
AloI	GAACNNNNNNTCC	2 cut(s)	2454, 2486
Alw21I	GWGCWC	4 cut(s)	1113, 1510, 3172, 3745
Alw26I	GTCTC	5 cut(s)	209, 962, 1506, 2069, 3498
AlwNI	CAGNNNCTG	3 cut(s)	859, 1037, 1121
Ama87I	CYCGRG	3 cut(s)	1503, 2730, 3888
AoxI	GGCC	8 cut(s)	171, 255, 404, 479, 1304, 1679, 2324, 2622
ApoI	RAATTY	6 cut(s)	107, 585, 1457, 1545, 1782, 2546
ArsI	GACNNNNNNTTYG	4 cut(s)	1457, 1489, 3301, 3333
AseI	ATTAAT	1 cut(s)	530
Asp700I	GAANNNNTTC	3 cut(s)	2817, 3073, 3513
AspLEI	GCGC	1 cut(s)	2610
AspS9I	GGNCC	8 cut(s)	479, 742, 944, 1871, 2324, 2623, 3892, 4071
AsuC2I	CCSGG	2 cut(s)	3889, 3890
AsuHPI	GGTGA	9 cut(s)	800, 1873, 2141, 2175, 2252, 2345, 2575, 2864, 3116
AsuNHI	GCTAGC	1 cut(s)	2369
AvaI	CYCGRG	3 cut(s)	1503, 2730, 3888
AvaII	GGWCC	5 cut(s)	742, 944, 1871, 3892, 4071
BaeI	ACNNNNGTAYC	2 cut(s)	4176, 4209
BalI	TGGCCA	1 cut(s)	257
BamHI	GGATCC	1 cut(s)	1345
BanI	GGYRCC	1 cut(s)	921
BanII	GRGCYC	3 cut(s)	1510, 2662, 3745
BarI	GAAGNNNNNNTAC	4 cut(s)	543, 575, 1905, 1937
Bbv12I	GWGCWC	4 cut(s)	1113, 1510, 3172, 3745
BbvCI	CCTCAGC	2 cut(s)	1258, 2093
BceAI	ACGGC	3 cut(s)	882, 3256, 4175
BcgI	CGANNNNNNTGC	4 cut(s)	977, 1011, 1803, 1837
BciT130I	CCWGG	6 cut(s)	324, 408, 798, 1875, 2876, 3331
BciVI	GTATCC	1 cut(s)	3109
BclI	TGATCA	3 cut(s)	865, 3208, 3217
BcnI	CCSGG	2 cut(s)	3889, 3890
BcoDI	GTCTC	5 cut(s)	209, 962, 1506, 2069, 3498
BfaI	CTAG	7 cut(s)	1511, 2370, 2450, 2501, 2571, 3528, 4010
BfmI	CTRYAG	7 cut(s)	904, 954, 963, 1051, 1612, 1698, 1882
BfuAI	ACCTGC	2 cut(s)	2194, 3686
BfuI	GTATCC	1 cut(s)	3109
BglII	AGATCT	4 cut(s)	1591, 2521, 2839, 3311
BmcAI	AGTACT	1 cut(s)	1073
Bme1390I	CCNGG	8 cut(s)	324, 408, 798, 1875, 2876, 3331, 3889, 3890
Bme18I	GGWCC	5 cut(s)	742, 944, 1871, 3892, 4071
BmeT110I	CYCGRG	3 cut(s)	1503, 2730, 3888
BmgT120I	GGNCC	8 cut(s)	479, 742, 944, 1871, 2324, 2623, 3892, 4071
BmiI	GGNNCC	9 cut(s)	321, 923, 945, 1347, 1872, 2325, 2659, 3819, 3893
BmrFI	CCNGG	8 cut(s)	324, 408, 798, 1875, 2876, 3331, 3889, 3890
BmrI	ACTGGG	3 cut(s)	30, 1109, 2309
BmtI	GCTAGC	1 cut(s)	2373
BmuI	ACTGGG	3 cut(s)	30, 1109, 2309
BpmI	CTGGAG	3 cut(s)	1461, 2214, 3664
Bpu10I	CCTNAGC	4 cut(s)	435, 537, 1258, 2093
BpuEI	CTTGAG	4 cut(s)	1790, 2639, 3612, 3860
BpuMI	CCSGG	2 cut(s)	3889, 3890
Bsa29I	ATCGAT	1 cut(s)	4180
BsaAI	YACGTR	1 cut(s)	61
BsaBI	GATNNNNATC	2 cut(s)	3437, 3618
BsaXI	ACNNNNNCTCC	2 cut(s)	943, 973
Bse118I	RCCGGY	1 cut(s)	837
Bse3DI	GCAATG	7 cut(s)	258, 702, 1749, 2235, 3060, 4040, 4092
Bse8I	GATNNNNATC	2 cut(s)	3437, 3618
BseBI	CCWGG	6 cut(s)	324, 408, 798, 1875, 2876, 3331
BseCI	ATCGAT	1 cut(s)	4180
BseGI	GGATG	9 cut(s)	79, 827, 1176, 1899, 1966, 3106, 3303, 3884, 3951
BseJI	GATNNNNATC	2 cut(s)	3437, 3618
BseMI	GCAATG	7 cut(s)	258, 702, 1749, 2235, 3060, 4040, 4092
BseRI	GAGGAG	2 cut(s)	953, 1572
BseYI	CCCAGC	1 cut(s)	691
BsgI	GTGCAG	3 cut(s)	1758, 3671, 3714
Bsh1236I	CGCG	2 cut(s)	176, 1935
BshFI	GGCC	8 cut(s)	173, 257, 406, 481, 1306, 1681, 2326, 2624
BshNI	GGYRCC	1 cut(s)	921
BshVI	ATCGAT	1 cut(s)	4180
BsiHKAI	GWGCWC	4 cut(s)	1113, 1510, 3172, 3745
BsiHKCI	CYCGRG	3 cut(s)	1503, 2730, 3888
BsiSI	CCGG	2 cut(s)	838, 3889
BslFI	GGGAC	2 cut(s)	1723, 3905
BsmAI	GTCTC	5 cut(s)	209, 962, 1506, 2069, 3498
BsmFI	GGGAC	2 cut(s)	1723, 3905
BsmI	GAATGC	3 cut(s)	460, 1449, 3754
BsnI	GGCC	8 cut(s)	173, 257, 406, 481, 1306, 1681, 2326, 2624
BsoBI	CYCGRG	3 cut(s)	1503, 2730, 3888
Bsp1286I	GDGCHC	5 cut(s)	1113, 1510, 2662, 3172, 3745
Bsp1407I	TGTACA	2 cut(s)	3871, 3909
Bsp19I	CCATGG	2 cut(s)	563, 2302
BspACI	CCGC	8 cut(s)	174, 176, 570, 919, 2509, 2595, 3235, 3540
BspANI	GGCC	8 cut(s)	173, 257, 406, 481, 1306, 1681, 2326, 2624
BspDI	ATCGAT	1 cut(s)	4180
BspFNI	CGCG	2 cut(s)	176, 1935
BspHI	TCATGA	3 cut(s)	1453, 3429, 3505
BspLI	GGNNCC	9 cut(s)	321, 923, 945, 1347, 1872, 2325, 2659, 3819, 3893
BspMAI	CTGCAG	1 cut(s)	908
BspMI	ACCTGC	2 cut(s)	2194, 3686
BspOI	GCTAGC	1 cut(s)	2373
BspQI	GCTCTTC	1 cut(s)	3789
BspT107I	GGYRCC	1 cut(s)	921
BsrBI	CCGCTC	1 cut(s)	570
BsrDI	GCAATG	7 cut(s)	258, 702, 1749, 2235, 3060, 4040, 4092
BsrFI	RCCGGY	1 cut(s)	837
BsrGI	TGTACA	2 cut(s)	3871, 3909
BssAI	RCCGGY	1 cut(s)	837
BssNAI	GTATAC	1 cut(s)	3879
BssT1I	CCWWGG	4 cut(s)	121, 563, 1685, 2302
Bst1107I	GTATAC	1 cut(s)	3879
Bst2UI	CCWGG	6 cut(s)	324, 408, 798, 1875, 2876, 3331
Bst6I	CTCTTC	5 cut(s)	564, 2181, 2751, 3789, 4086
BstAPI	GCANNNNNTGC	3 cut(s)	455, 2437, 4115
BstAUI	TGTACA	2 cut(s)	3871, 3909
BstBAI	YACGTR	1 cut(s)	61
BstC8I	GCNNGC	8 cut(s)	839, 1406, 2036, 2087, 2235, 2371, 3462, 3741
BstDSI	CCRYGG	3 cut(s)	174, 563, 2302
BstEII	GGTNACC	1 cut(s)	686
BstF5I	GGATG	9 cut(s)	79, 827, 1176, 1899, 1966, 3106, 3303, 3884, 3951
BstFNI	CGCG	2 cut(s)	176, 1935
BstHHI	GCGC	1 cut(s)	2610
BstMAI	GTCTC	5 cut(s)	209, 962, 1506, 2069, 3498
BstNI	CCWGG	6 cut(s)	324, 408, 798, 1875, 2876, 3331
BstNSI	RCATGY	5 cut(s)	392, 581, 1484, 1604, 1808
BstPI	GGTNACC	1 cut(s)	686
BstSCI	CCNGG	8 cut(s)	322, 406, 796, 1873, 2874, 3329, 3887, 3888
BstSFI	CTRYAG	7 cut(s)	904, 954, 963, 1051, 1612, 1698, 1882
BstSNI	TACGTA	1 cut(s)	61
BstUI	CGCG	2 cut(s)	176, 1935
BstX2I	RGATCY	8 cut(s)	800, 1345, 1591, 2521, 2839, 3157, 3311, 3524
BstXI	CCANNNNNNTGG	1 cut(s)	1416
BstYI	RGATCY	8 cut(s)	800, 1345, 1591, 2521, 2839, 3157, 3311, 3524
BstZ17I	GTATAC	1 cut(s)	3879
Bsu15I	ATCGAT	1 cut(s)	4180
BsuI	GTATCC	1 cut(s)	3109
BsuRI	GGCC	8 cut(s)	173, 257, 406, 481, 1306, 1681, 2326, 2624
BsuTUI	ATCGAT	1 cut(s)	4180
BtgI	CCRYGG	3 cut(s)	174, 563, 2302
BtgZI	GCGATG	2 cut(s)	1950, 3627
BtsCI	GGATG	9 cut(s)	79, 827, 1176, 1899, 1966, 3106, 3303, 3884, 3951
BtsI	GCAGTG	4 cut(s)	913, 1332, 2617, 3308
BveI	ACCTGC	2 cut(s)	2194, 3686
Cac8I	GCNNGC	8 cut(s)	839, 1406, 2036, 2087, 2235, 2371, 3462, 3741
CaiI	CAGNNNCTG	3 cut(s)	859, 1037, 1121
CciI	TCATGA	3 cut(s)	1453, 3429, 3505
CfoI	GCGC	1 cut(s)	2610
Cfr10I	RCCGGY	1 cut(s)	837
Cfr13I	GGNCC	8 cut(s)	479, 742, 944, 1871, 2324, 2623, 3892, 4071
Cfr42I	CCGCGG	1 cut(s)	177
Cfr9I	CCCGGG	1 cut(s)	3888
ClaI	ATCGAT	1 cut(s)	4180
CseI	GACGC	2 cut(s)	3245, 3797
CspCI	CAANNNNNGTGG	2 cut(s)	1619, 1654
DraI	TTTAAA	2 cut(s)	1399, 1720
DraIII	CACNNNGTG	1 cut(s)	1125
DrdI	GACNNNNNNGTC	1 cut(s)	2721
DseDI	GACNNNNNNGTC	1 cut(s)	2721
EaeI	YGGCCR	2 cut(s)	171, 255
Eam1104I	CTCTTC	5 cut(s)	564, 2181, 2751, 3789, 4086
EarI	CTCTTC	5 cut(s)	564, 2181, 2751, 3789, 4086
Ecl136II	GAGCTC	2 cut(s)	1508, 3743
Eco105I	TACGTA	1 cut(s)	61
Eco130I	CCWWGG	4 cut(s)	121, 563, 1685, 2302
Eco147I	AGGCCT	1 cut(s)	1681
Eco24I	GRGCYC	3 cut(s)	1510, 2662, 3745
Eco47I	GGWCC	5 cut(s)	742, 944, 1871, 3892, 4071
Eco53kI	GAGCTC	2 cut(s)	1508, 3743
Eco57I	CTGAAG	7 cut(s)	576, 1559, 2024, 2238, 2459, 3212, 3584
Eco88I	CYCGRG	3 cut(s)	1503, 2730, 3888
Eco91I	GGTNACC	1 cut(s)	686
EcoICRI	GAGCTC	2 cut(s)	1508, 3743
EcoO109I	RGGNCCY	2 cut(s)	2324, 4071
EcoO65I	GGTNACC	1 cut(s)	686
EcoRI	GAATTC	2 cut(s)	107, 1457
EcoRII	CCWGG	6 cut(s)	322, 406, 796, 1873, 2874, 3329
EcoT14I	CCWWGG	4 cut(s)	121, 563, 1685, 2302
EcoT22I	ATGCAT	2 cut(s)	451, 3561
EcoT38I	GRGCYC	3 cut(s)	1510, 2662, 3745
ErhI	CCWWGG	4 cut(s)	121, 563, 1685, 2302
FalI	AAGNNNNNCTT	6 cut(s)	1669, 1701, 2655, 2687, 3177, 3209
FaqI	GGGAC	2 cut(s)	1723, 3905
FauI	CCCGC	3 cut(s)	2516, 2602, 3228
FauNDI	CATATG	2 cut(s)	2140, 4143
FbaI	TGATCA	3 cut(s)	865, 3208, 3217
FblI	GTMKAC	2 cut(s)	2928, 3878
FokI	GGATG	9 cut(s)	66, 814, 1183, 1906, 1973, 3113, 3310, 3871, 3958
FriOI	GRGCYC	3 cut(s)	1510, 2662, 3745
FspBI	CTAG	7 cut(s)	1511, 2370, 2450, 2501, 2571, 3528, 4010
FspI	TGCGCA	1 cut(s)	2609
GlaI	GCGC	1 cut(s)	2609
GsaI	CCCAGC	1 cut(s)	695
GsuI	CTGGAG	3 cut(s)	1461, 2214, 3664
HaeIII	GGCC	8 cut(s)	173, 257, 406, 481, 1306, 1681, 2326, 2624
HapII	CCGG	2 cut(s)	838, 3889
HgaI	GACGC	2 cut(s)	3245, 3797
HhaI	GCGC	1 cut(s)	2610
Hin6I	GCGC	1 cut(s)	2608
HinP1I	GCGC	1 cut(s)	2608
HincII	GTYRAC	2 cut(s)	27, 3020
HindII	GTYRAC	2 cut(s)	27, 3020
HindIII	AAGCTT	3 cut(s)	2255, 2308, 3851
HpaII	CCGG	2 cut(s)	838, 3889
HphI	GGTGA	9 cut(s)	800, 1873, 2141, 2175, 2252, 2345, 2575, 2864, 3116
Hpy99I	CGWCG	1 cut(s)	3261
HpyCH4IV	ACGT	2 cut(s)	60, 549
HpySE526I	ACGT	2 cut(s)	60, 549
HspAI	GCGC	1 cut(s)	2608
KroI	GCCGGC	1 cut(s)	837
KroNI	GCCGGC	1 cut(s)	839
Ksp22I	TGATCA	3 cut(s)	865, 3208, 3217
KspI	CCGCGG	1 cut(s)	177
LguI	GCTCTTC	1 cut(s)	3789
MaeI	CTAG	7 cut(s)	1511, 2370, 2450, 2501, 2571, 3528, 4010
MaeII	ACGT	2 cut(s)	60, 549
MbiI	CCGCTC	1 cut(s)	570
MfeI	CAATTG	1 cut(s)	3396
MflI	RGATCY	8 cut(s)	800, 1345, 1591, 2521, 2839, 3157, 3311, 3524
MhlI	GDGCHC	5 cut(s)	1113, 1510, 2662, 3172, 3745
MlsI	TGGCCA	1 cut(s)	257
MluNI	TGGCCA	1 cut(s)	257
MlyI	GAGTC	5 cut(s)	1460, 1508, 1705, 1910, 3435
MmeI	TCCRAC	8 cut(s)	963, 1006, 1623, 1684, 1938, 2354, 3825, 4077
Mox20I	TGGCCA	1 cut(s)	257
Mph1103I	ATGCAT	2 cut(s)	451, 3561
MroNI	GCCGGC	1 cut(s)	837
MroXI	GAANNNNTTC	3 cut(s)	2817, 3073, 3513
MscI	TGGCCA	1 cut(s)	257
MslI	CAYNNNNRTG	6 cut(s)	393, 750, 897, 1230, 1809, 2877
Msp20I	TGGCCA	1 cut(s)	257
MspA1I	CMGCKG	2 cut(s)	176, 2000
MspI	CCGG	2 cut(s)	838, 3889
MspR9I	CCNGG	8 cut(s)	324, 408, 798, 1875, 2876, 3331, 3889, 3890
MunI	CAATTG	1 cut(s)	3396
Mva1269I	GAATGC	3 cut(s)	460, 1449, 3754
MvaI	CCWGG	6 cut(s)	324, 408, 798, 1875, 2876, 3331
MvnI	CGCG	2 cut(s)	176, 1935
NaeI	GCCGGC	1 cut(s)	839
NciI	CCSGG	2 cut(s)	3889, 3890
NcoI	CCATGG	2 cut(s)	563, 2302
NdeI	CATATG	2 cut(s)	2140, 4143
NgoMIV	GCCGGC	1 cut(s)	837
NheI	GCTAGC	1 cut(s)	2369
NlaIV	GGNNCC	9 cut(s)	321, 923, 945, 1347, 1872, 2325, 2659, 3819, 3893
NmeAIII	GCCGAG	1 cut(s)	2170
NmuCI	GTSAC	5 cut(s)	899, 1117, 1970, 2129, 2786
NsbI	TGCGCA	1 cut(s)	2609
NsiI	ATGCAT	2 cut(s)	451, 3561
NspI	RCATGY	5 cut(s)	392, 581, 1484, 1604, 1808
OliI	CACNNNNGTG	1 cut(s)	2877
PaeR7I	CTCGAG	1 cut(s)	1503
PagI	TCATGA	3 cut(s)	1453, 3429, 3505
PaqCI	CACCTGC	2 cut(s)	2194, 3686
PceI	AGGCCT	1 cut(s)	1681
PciI	ACATGT	4 cut(s)	388, 577, 1480, 1804
PciSI	GCTCTTC	1 cut(s)	3789
PctI	GAATGC	3 cut(s)	460, 1449, 3754
PdiI	GCCGGC	1 cut(s)	839
PdmI	GAANNNNTTC	3 cut(s)	2817, 3073, 3513
PfeI	GAWTC	7 cut(s)	276, 297, 1141, 1295, 2392, 2540, 3509
PflFI	GACNNNGTC	1 cut(s)	205
PflMI	CCANNNNNTGG	1 cut(s)	1355
PfoI	TCCNGGA	2 cut(s)	796, 1873
PleI	GAGTC	5 cut(s)	1460, 1507, 1705, 1909, 3434
PpsI	GAGTC	5 cut(s)	1460, 1507, 1705, 1909, 3434
Ppu21I	YACGTR	1 cut(s)	61
PpuMI	RGGWCCY	1 cut(s)	4071
PscI	ACATGT	4 cut(s)	388, 577, 1480, 1804
PshBI	ATTAAT	1 cut(s)	530
PsiI	TTATAA	1 cut(s)	815
Psp124BI	GAGCTC	2 cut(s)	1510, 3745
Psp5II	RGGWCCY	1 cut(s)	4071
Psp6I	CCWGG	6 cut(s)	322, 406, 796, 1873, 2874, 3329
PspEI	GGTNACC	1 cut(s)	686
PspFI	CCCAGC	1 cut(s)	691
PspGI	CCWGG	6 cut(s)	322, 406, 796, 1873, 2874, 3329
PspN4I	GGNNCC	9 cut(s)	321, 923, 945, 1347, 1872, 2325, 2659, 3819, 3893
PspPI	GGNCC	8 cut(s)	479, 742, 944, 1871, 2324, 2623, 3892, 4071
PspPPI	RGGWCCY	1 cut(s)	4071
PstI	CTGCAG	1 cut(s)	908
PstNI	CAGNNNCTG	3 cut(s)	859, 1037, 1121
PsuI	RGATCY	8 cut(s)	800, 1345, 1591, 2521, 2839, 3157, 3311, 3524
PsyI	GACNNNGTC	1 cut(s)	205
PvuII	CAGCTG	1 cut(s)	2000
RseI	CAYNNNNRTG	6 cut(s)	393, 750, 897, 1230, 1809, 2877
SacI	GAGCTC	2 cut(s)	1510, 3745
SacII	CCGCGG	1 cut(s)	177
SapI	GCTCTTC	1 cut(s)	3789
Sau96I	GGNCC	8 cut(s)	479, 742, 944, 1871, 2324, 2623, 3892, 4071
ScaI	AGTACT	1 cut(s)	1073
SchI	GAGTC	5 cut(s)	1460, 1508, 1705, 1910, 3435
ScrFI	CCNGG	8 cut(s)	324, 408, 798, 1875, 2876, 3331, 3889, 3890
SduI	GDGCHC	5 cut(s)	1113, 1510, 2662, 3172, 3745
SfcI	CTRYAG	7 cut(s)	904, 954, 963, 1051, 1612, 1698, 1882
Sfr274I	CTCGAG	1 cut(s)	1503
Sfr303I	CCGCGG	1 cut(s)	177
SgrBI	CCGCGG	1 cut(s)	177
SinI	GGWCC	5 cut(s)	742, 944, 1871, 3892, 4071
SlaI	CTCGAG	1 cut(s)	1503
SmaI	CCCGGG	1 cut(s)	3890
SmiMI	CAYNNNNRTG	6 cut(s)	393, 750, 897, 1230, 1809, 2877
SmlI	CTYRAG	5 cut(s)	1503, 1769, 2618, 3627, 3839
SmoI	CTYRAG	5 cut(s)	1503, 1769, 2618, 3627, 3839
SnaBI	TACGTA	1 cut(s)	61
SseBI	AGGCCT	1 cut(s)	1681
SsiI	CCGC	8 cut(s)	174, 176, 570, 919, 2509, 2595, 3235, 3540
SspI	AATATT	1 cut(s)	810
SspMI	CTAG	7 cut(s)	1511, 2370, 2450, 2501, 2571, 3528, 4010
SstI	GAGCTC	2 cut(s)	1510, 3745
StuI	AGGCCT	1 cut(s)	1681
StyD4I	CCNGG	8 cut(s)	322, 406, 796, 1873, 2874, 3329, 3887, 3888
StyI	CCWWGG	4 cut(s)	121, 563, 1685, 2302
TaiI	ACGT	2 cut(s)	63, 552
TaqI	TCGA	6 cut(s)	997, 1096, 1278, 1504, 2821, 4180
TatI	WGTACW	4 cut(s)	1071, 1381, 3871, 3909
TauI	GCSGC	3 cut(s)	176, 922, 3543
TfiI	GAWTC	7 cut(s)	276, 297, 1141, 1295, 2392, 2540, 3509
TseFI	GTSAC	5 cut(s)	899, 1117, 1970, 2129, 2786
Tsp45I	GTSAC	5 cut(s)	899, 1117, 1970, 2129, 2786
TspGWI	ACGGA	1 cut(s)	2046
TspMI	CCCGGG	1 cut(s)	3888
Tth111I	GACNNNGTC	1 cut(s)	205
Van91I	CCANNNNNTGG	1 cut(s)	1355
VpaK11BI	GGWCC	5 cut(s)	742, 944, 1871, 3892, 4071
VspI	ATTAAT	1 cut(s)	530
XapI	RAATTY	6 cut(s)	107, 585, 1457, 1545, 1782, 2546
XceI	RCATGY	5 cut(s)	392, 581, 1484, 1604, 1808
XcmI	CCANNNNNNNNNTGG	3 cut(s)	50, 1122, 1163
XhoI	CTCGAG	1 cut(s)	1503
XmaI	CCCGGG	1 cut(s)	3888
XmiI	GTMKAC	2 cut(s)	2928, 3878
XmnI	GAANNNNTTC	3 cut(s)	2817, 3073, 3513
XspI	CTAG	7 cut(s)	1511, 2370, 2450, 2501, 2571, 3528, 4010
ZrmI	AGTACT	1 cut(s)	1073
Zsp2I	ATGCAT	2 cut(s)	451, 3561

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.