pycom16g02030

TMEM214, C-terminal, caspase 4 activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002090
Physical Location & Seq
Reverse (-)
1271939 .. 1274676
2738 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1266 bp
ATGCGTTTCGCGGACTATTTCGGCCGTGCATTCTCTGCGGTGTCTGCCTCTCAGTTTCCATGGGTGAAGATGTTCAAGGAGTCATCGGTGGCCAAGCTTGCCGATATCCATCTTTCTCATATTTCTGAGGATGTTTATAAGACATCAATTGACTGGATCAACCAGCGTTCCGTTGAGGCCCTTGGGTCTTTTATATTATGGTCCTTAGACAGCATTCTTGCAGACCTAGCGAGCCAAGTTTCTGGCACAAAGGGCAAGAAGGCTGTGCAGAATGTGTCTTCAAAATCTCAGGTTGCGATATTTGTGGTTGTAGCAATGGTATTGCGAAGAAAACCCGATGTCTTGATTAGTATATTGCCAACACTGAGAGAAAATTCAAAGTATCAAGGACAAGATAAGCTTCCAGTAATTATATGGATGATAGTTCAGGCTAGTCAAGGGGACCTCGCCGTTGGGTTGCATGCATGGGCCCGCAATGTGTTGCCTCTAGTCTGTGGAAAAAGTAGTAATCCACAGTCCCGTGATCTTGTTTTGCAGTTAGTGGAGAGAATTCTGTCTATGCCCAAGGCTCGTACTATTTTAGTAAATAGTGCTGTTAGGAAGGGGGAGCGTTTGGTGCCACCTTCTGCATTAGAGATACTTATGGGAGTTACCTTTCCTGCACATTCAGCTAGAGTAAAGGCCACCGAAAGGTTCGAGGCTATATATCCAACCCTAAAAGCGGTCGCTCTAGCTGGCGCCCCTGGAAGCAAAGCAATGAAACAAGTTTCACAGCAGATAATGAGTTTTGCTGTCAAAGCTGCAGGAGAAAGTATACCTGAGCTATCCAATGAAGCAACTGGAATTTTCATTTGGTGTTTGACCCAACATGCTGATTGCTTCAAGCAGTGGGACAAGGTCTACGAGGAAAACTTAGAAGTGAGTGTCGCTGTTCTGAAGAAACTTTCTAACCAATGGAAGGAACATTCTGCAAACCTCTCTCCTCTTGATCCTATGAGGGAAGCTCTCAAAAGTTTTATGCGCAAGAATAAGAAGGCATTGGCTGGTGAGGCGGAGGATGTACACCGAGAAAAGCTTAGCAAGGAAGCAGACAAGTATTGTAAGACATTATTGGCAAAGCTATCACAGGGTAGCGGGTTCAAGAAGACTCTGGCCGTTGCTGTTGTAGCCTTGGCTGTTGGTGCTGCTGTCATGTCCCCGAACATGGAATCTTGGGAATTGGAGTTGAAGAAACTAACTGCGACCATCAGCTCTTTCTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TMEM214

Protein Analysis

422

Amino Acids

46.36

Weight (kDa)

9.48

Isoelectric Point (pI)

33.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TMEM214 PF10151 103 - 396 9.4e-18 TMEM214, C-terminal, caspase 4 activator
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 138
Acc16I TGCGCA 1 cut(s) 1022
AccB1I GGYRCC 2 cut(s) 616, 737
AccI GTMKAC 2 cut(s) 814, 900
AccII CGCG 1 cut(s) 11
AciI CCGC 6 cut(s) 11, 38, 472, 722, 1052, 1134
AclWI GGATC 2 cut(s) 164, 983
AcoI YGGCCR 3 cut(s) 22, 90, 1152
AcsI RAATTY 3 cut(s) 373, 549, 843
AcuI CTGAAG 1 cut(s) 956
AcyI GRCGYC 1 cut(s) 738
AfaI GTAC 2 cut(s) 574, 1062
AfiI CCNNNNNNNGG 4 cut(s) 690, 721, 958, 1204
AgsI TTSAA 7 cut(s) 76, 282, 378, 883, 1141, 1228, 1262
AjnI CCWGG 1 cut(s) 742
AjuI GAANNNNNNNTTGG 2 cut(s) 835, 867
AlwI GGATC 2 cut(s) 164, 983
AoxI GGCC 6 cut(s) 22, 90, 177, 468, 681, 1152
ApaI GGGCCC 1 cut(s) 472
ApeKI GCWGC 2 cut(s) 800, 1184
ApoI RAATTY 3 cut(s) 373, 549, 843
Asp700I GAANNNNTTC 1 cut(s) 71
AspLEI GCGC 2 cut(s) 740, 1023
AspS9I GGNCC 5 cut(s) 178, 201, 442, 468, 469
AsuHPI GGTGA 2 cut(s) 76, 1058
AvaII GGWCC 2 cut(s) 201, 442
BaeGI GKGCMC 1 cut(s) 472
BalI TGGCCA 1 cut(s) 92
BanI GGYRCC 2 cut(s) 616, 737
BanII GRGCYC 1 cut(s) 472
BbsI GAAGAC 2 cut(s) 270, 1151
BbvI GCAGC 2 cut(s) 787, 1171
BccI CCATC 2 cut(s) 117, 1253
BceAI ACGGC 3 cut(s) 9, 434, 1139
BciT130I CCWGG 1 cut(s) 744
BfaI CTAG 5 cut(s) 227, 432, 488, 672, 731
BfmI CTRYAG 1 cut(s) 801
BfoI RGCGCY 1 cut(s) 741
BisI GCNGC 2 cut(s) 801, 1185
BlpI GCTNAGC 1 cut(s) 1076
BlsI GCNGC 2 cut(s) 802, 1186
Bme1390I CCNGG 1 cut(s) 744
Bme18I GGWCC 2 cut(s) 201, 442
BmgT120I GGNCC 5 cut(s) 178, 201, 442, 468, 469
BmiI GGNNCC 4 cut(s) 443, 470, 618, 739
BmrFI CCNGG 1 cut(s) 744
BpiI GAAGAC 2 cut(s) 270, 1151
BplI GAGNNNNNCTC 2 cut(s) 988, 1020
Bpu10I CCTNAGC 1 cut(s) 819
Bpu1102I GCTNAGC 1 cut(s) 1076
BsaBI GATNNNNATC 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 738
BsaJI CCNNGG 5 cut(s) 59, 181, 564, 742, 1170
Bsc4I CCNNNNNNNGG 4 cut(s) 690, 721, 958, 1204
Bse1I ACTGG 3 cut(s) 158, 404, 844
Bse3DI GCAATG 3 cut(s) 321, 481, 762
Bse8I GATNNNNATC 1 cut(s) 108
BseBI CCWGG 1 cut(s) 744
BseDI CCNNGG 5 cut(s) 59, 181, 564, 742, 1170
BseGI GGATG 3 cut(s) 136, 423, 1063
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 4 cut(s) 690, 721, 958, 1204
BseMI GCAATG 3 cut(s) 321, 481, 762
BseMII CTCAG 5 cut(s) 65, 117, 302, 356, 810
BseNI ACTGG 3 cut(s) 158, 404, 844
BseRI GAGGAG 1 cut(s) 972
BseSI GKGCMC 1 cut(s) 472
BseX3I CGGCCG 1 cut(s) 22
BseXI GCAGC 2 cut(s) 787, 1171
BsgI GTGCAG 2 cut(s) 287, 645
Bsh1236I CGCG 1 cut(s) 11
Bsh1285I CGRYCG 2 cut(s) 25, 726
BshFI GGCC 6 cut(s) 24, 92, 179, 470, 683, 1154
BshNI GGYRCC 2 cut(s) 616, 737
BsiEI CGRYCG 2 cut(s) 25, 726
BslFI GGGAC 4 cut(s) 455, 502, 905, 1180
BslI CCNNNNNNNGG 4 cut(s) 690, 721, 958, 1204
BsmFI GGGAC 4 cut(s) 455, 502, 905, 1180
BsmI GAATGC 2 cut(s) 29, 213
BsnI GGCC 6 cut(s) 24, 92, 179, 470, 683, 1154
Bsp120I GGGCCC 1 cut(s) 468
Bsp1286I GDGCHC 1 cut(s) 472
Bsp1407I TGTACA 1 cut(s) 1060
Bsp143I GATC 3 cut(s) 156, 523, 988
Bsp1720I GCTNAGC 1 cut(s) 1076
Bsp19I CCATGG 1 cut(s) 59
BspACI CCGC 6 cut(s) 11, 38, 472, 722, 1052, 1134
BspANI GGCC 6 cut(s) 24, 92, 179, 470, 683, 1154
BspCNI CTCAG 5 cut(s) 64, 118, 301, 357, 811
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 4 cut(s) 443, 470, 618, 739
BspMAI CTGCAG 1 cut(s) 805
BspPI GGATC 2 cut(s) 164, 983
BspT107I GGYRCC 2 cut(s) 616, 737
BsrDI GCAATG 3 cut(s) 321, 481, 762
BsrGI TGTACA 1 cut(s) 1060
BsrI ACTGG 3 cut(s) 158, 404, 844
BssECI CCNNGG 5 cut(s) 59, 181, 564, 742, 1170
BssMI GATC 3 cut(s) 156, 523, 988
BssNAI GTATAC 1 cut(s) 815
BssNI GRCGYC 1 cut(s) 738
BssT1I CCWWGG 4 cut(s) 59, 181, 564, 1170
Bst1107I GTATAC 1 cut(s) 815
Bst2UI CCWGG 1 cut(s) 744
Bst4CI ACNGT 1 cut(s) 516
BstACI GRCGYC 1 cut(s) 738
BstAPI GCANNNNNTGC 1 cut(s) 35
BstAUI TGTACA 1 cut(s) 1060
BstC8I GCNNGC 5 cut(s) 99, 232, 462, 472, 736
BstDEI CTNAG 8 cut(s) 51, 126, 205, 288, 365, 819, 913, 1076
BstDSI CCRYGG 1 cut(s) 59
BstF5I GGATG 3 cut(s) 136, 423, 1063
BstFNI CGCG 1 cut(s) 11
BstH2I RGCGCY 1 cut(s) 741
BstHHI GCGC 2 cut(s) 740, 1023
BstKTI GATC 3 cut(s) 159, 526, 991
BstMBI GATC 3 cut(s) 156, 523, 988
BstMCI CGRYCG 2 cut(s) 25, 726
BstNI CCWGG 1 cut(s) 744
BstNSI RCATGY 2 cut(s) 464, 872
BstSCI CCNGG 1 cut(s) 742
BstSFI CTRYAG 1 cut(s) 801
BstSLI GKGCMC 1 cut(s) 472
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 2 cut(s) 787, 1171
BstV2I GAAGAC 2 cut(s) 270, 1151
BstXI CCANNNNNNTGG 1 cut(s) 242
BstZ17I GTATAC 1 cut(s) 815
BstZI CGGCCG 1 cut(s) 22
BsuRI GGCC 6 cut(s) 24, 92, 179, 470, 683, 1154
BtgI CCRYGG 1 cut(s) 59
BtsCI GGATG 3 cut(s) 136, 423, 1063
BtsI GCAGTG 1 cut(s) 893
BtsIMutI CAGTG 2 cut(s) 362, 893
Cac8I GCNNGC 5 cut(s) 99, 232, 462, 472, 736
CfoI GCGC 2 cut(s) 740, 1023
Cfr13I GGNCC 5 cut(s) 178, 201, 442, 468, 469
Csp6I GTAC 2 cut(s) 573, 1061
CviAII CATG 6 cut(s) 60, 461, 465, 869, 1192, 1204
CviQI GTAC 2 cut(s) 573, 1061
DdeI CTNAG 8 cut(s) 51, 126, 205, 288, 365, 819, 913, 1076
DinI GGCGCC 1 cut(s) 739
DpnI GATC 3 cut(s) 158, 525, 990
DpnII GATC 3 cut(s) 156, 523, 988
EaeI YGGCCR 3 cut(s) 22, 90, 1152
EagI CGGCCG 1 cut(s) 22
EciI GGCGGA 1 cut(s) 1067
EclXI CGGCCG 1 cut(s) 22
Eco130I CCWWGG 4 cut(s) 59, 181, 564, 1170
Eco24I GRGCYC 1 cut(s) 472
Eco32I GATATC 1 cut(s) 106
Eco47I GGWCC 2 cut(s) 201, 442
Eco52I CGGCCG 1 cut(s) 22
Eco57I CTGAAG 1 cut(s) 956
EcoO109I RGGNCCY 2 cut(s) 178, 442
EcoRI GAATTC 1 cut(s) 549
EcoRII CCWGG 1 cut(s) 742
EcoRV GATATC 1 cut(s) 106
EcoT14I CCWWGG 4 cut(s) 59, 181, 564, 1170
EcoT22I ATGCAT 1 cut(s) 466
EcoT38I GRGCYC 1 cut(s) 472
EgeI GGCGCC 1 cut(s) 739
EheI GGCGCC 1 cut(s) 739
ErhI CCWWGG 4 cut(s) 59, 181, 564, 1170
FaeI CATG 6 cut(s) 63, 464, 468, 872, 1195, 1207
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 4 cut(s) 455, 502, 905, 1180
FatI CATG 6 cut(s) 59, 460, 464, 868, 1191, 1203
FauI CCCGC 2 cut(s) 479, 1127
FblI GTMKAC 2 cut(s) 814, 900
Fnu4HI GCNGC 2 cut(s) 801, 1185
FokI GGATG 3 cut(s) 143, 430, 1070
FriOI GRGCYC 1 cut(s) 472
Fsp4HI GCNGC 2 cut(s) 801, 1185
FspBI CTAG 5 cut(s) 227, 432, 488, 672, 731
FspI TGCGCA 1 cut(s) 1022
GlaI GCGC 2 cut(s) 739, 1022
GluI GCNGC 2 cut(s) 801, 1185
HaeII RGCGCY 1 cut(s) 741
HaeIII GGCC 6 cut(s) 24, 92, 179, 470, 683, 1154
HhaI GCGC 2 cut(s) 740, 1023
Hin1I GRCGYC 1 cut(s) 738
Hin1II CATG 6 cut(s) 63, 464, 468, 872, 1195, 1207
Hin6I GCGC 2 cut(s) 738, 1021
HinP1I GCGC 2 cut(s) 738, 1021
HindIII AAGCTT 3 cut(s) 95, 398, 1073
HinfI GANTC 3 cut(s) 80, 1147, 1208
HphI GGTGA 2 cut(s) 76, 1058
Hpy166II GTNNAC 3 cut(s) 815, 901, 1063
Hpy188I TCNGA 2 cut(s) 127, 936
Hpy188III TCNNGA 3 cut(s) 343, 986, 1141
Hpy8I GTNNAC 3 cut(s) 815, 901, 1063
HpyAV CCTTC 5 cut(s) 253, 595, 633, 952, 1027
HpyCH4III ACNGT 1 cut(s) 516
HpyF3I CTNAG 8 cut(s) 51, 126, 205, 288, 365, 819, 913, 1076
Hsp92I GRCGYC 1 cut(s) 738
Hsp92II CATG 6 cut(s) 63, 464, 468, 872, 1195, 1207
HspAI GCGC 2 cut(s) 738, 1021
KasI GGCGCC 1 cut(s) 737
Kzo9I GATC 3 cut(s) 156, 523, 988
LmnI GCTCC 1 cut(s) 607
Lsp1109I GCAGC 2 cut(s) 787, 1171
MaeI CTAG 5 cut(s) 227, 432, 488, 672, 731
MaeIII GTNAC 1 cut(s) 649
MalI GATC 3 cut(s) 158, 525, 990
MboI GATC 3 cut(s) 156, 523, 988
MboII GAAGA 6 cut(s) 79, 270, 339, 949, 1156, 1240
MfeI CAATTG 1 cut(s) 147
MhlI GDGCHC 1 cut(s) 472
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 6 cut(s) 147, 373, 408, 549, 843, 1217
MluNI TGGCCA 1 cut(s) 92
Mly113I GGCGCC 1 cut(s) 738
MlyI GAGTC 2 cut(s) 89, 1141
MmeI TCCRAC 1 cut(s) 734
Mox20I TGGCCA 1 cut(s) 92
Mph1103I ATGCAT 1 cut(s) 466
MroXI GAANNNNTTC 1 cut(s) 71
MscI TGGCCA 1 cut(s) 92
Msp20I TGGCCA 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 744
MunI CAATTG 1 cut(s) 147
Mva1269I GAATGC 2 cut(s) 29, 213
MvaI CCWGG 1 cut(s) 744
MvnI CGCG 1 cut(s) 11
NarI GGCGCC 1 cut(s) 738
NcoI CCATGG 1 cut(s) 59
NdeII GATC 3 cut(s) 156, 523, 988
NlaIII CATG 6 cut(s) 63, 464, 468, 872, 1195, 1207
NlaIV GGNNCC 4 cut(s) 443, 470, 618, 739
NsbI TGCGCA 1 cut(s) 1022
NsiI ATGCAT 1 cut(s) 466
NspI RCATGY 2 cut(s) 464, 872
PaeI GCATGC 1 cut(s) 464
PctI GAATGC 2 cut(s) 29, 213
PdmI GAANNNNTTC 1 cut(s) 71
PfeI GAWTC 1 cut(s) 1208
PflFI GACNNNGTC 1 cut(s) 896
PkrI GCNGC 2 cut(s) 802, 1186
PleI GAGTC 2 cut(s) 88, 1141
PluTI GGCGCC 1 cut(s) 741
PpsI GAGTC 2 cut(s) 88, 1141
PpuMI RGGWCCY 1 cut(s) 442
PsiI TTATAA 1 cut(s) 138
Psp5II RGGWCCY 1 cut(s) 442
Psp6I CCWGG 1 cut(s) 742
PspGI CCWGG 1 cut(s) 742
PspN4I GGNNCC 4 cut(s) 443, 470, 618, 739
PspOMI GGGCCC 1 cut(s) 468
PspPI GGNCC 5 cut(s) 178, 201, 442, 468, 469
PspPPI RGGWCCY 1 cut(s) 442
PstI CTGCAG 1 cut(s) 805
PsyI GACNNNGTC 1 cut(s) 896
RsaI GTAC 2 cut(s) 574, 1062
RsaNI GTAC 2 cut(s) 573, 1061
SatI GCNGC 2 cut(s) 801, 1185
Sau3AI GATC 3 cut(s) 156, 523, 988
Sau96I GGNCC 5 cut(s) 178, 201, 442, 468, 469
SchI GAGTC 2 cut(s) 89, 1141
ScrFI CCNGG 1 cut(s) 744
SduI GDGCHC 1 cut(s) 472
SfcI CTRYAG 1 cut(s) 801
SfoI GGCGCC 1 cut(s) 739
SinI GGWCC 2 cut(s) 201, 442
SphI GCATGC 1 cut(s) 464
Sse9I AATT 6 cut(s) 147, 373, 408, 549, 843, 1217
SsiI CCGC 6 cut(s) 11, 38, 472, 722, 1052, 1134
SspDI GGCGCC 1 cut(s) 737
SspMI CTAG 5 cut(s) 227, 432, 488, 672, 731
StyD4I CCNGG 1 cut(s) 742
StyI CCWWGG 4 cut(s) 59, 181, 564, 1170
TaaI ACNGT 1 cut(s) 516
TaqI TCGA 1 cut(s) 696
TasI AATT 6 cut(s) 147, 373, 408, 549, 843, 1217
TatI WGTACW 1 cut(s) 1060
TfiI GAWTC 1 cut(s) 1208
TscAI CASTG 2 cut(s) 369, 893
TseI GCWGC 2 cut(s) 800, 1184
TspDTI ATGAA 3 cut(s) 773, 838, 846
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 2 cut(s) 369, 893
Tth111I GACNNNGTC 1 cut(s) 896
VpaK11BI GGWCC 2 cut(s) 201, 442
XapI RAATTY 3 cut(s) 373, 549, 843
XceI RCATGY 2 cut(s) 464, 872
XcmI CCANNNNNNNNNTGG 1 cut(s) 411
XmiI GTMKAC 2 cut(s) 814, 900
XmnI GAANNNNTTC 1 cut(s) 71
XspI CTAG 5 cut(s) 227, 432, 488, 672, 731
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.