pycom16g03140

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0001685
Physical Location & Seq
Reverse (-)
1974600 .. 1975058
459 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g03140.1

Sequence Viewer

Length: 459 bp
ATGGAGATTGTTCCGACTACGTACTACGACAACTTGAAGAGGTACTGGAGGAGGAGAAGGTACCAAAAGCTGAATGGTGAGCCTAGAAAGAAGATGAGGGTCACAAGGCTCGGCGGAGGAGCTAAAGGGAGCACTAGTCCTAAACGGAGTTGGAAGCTCAAGACCAAGCTGAGGCTGATGAAATTTGTTTCACCAATAAAGCTTTTGACAAAGTTGCATAACGGTTACGTGGACCTGATGATTCGGATGGCGGGAAAGGCCGGCGGCGTTGGGAGGATTTCCGCGAAGAAGATTGCAAAACCCCAGGATCAGGTTTCAGTGGCTGCCTGTGGTGATCAGTTAGTTGACAGCAGATTGGTTTTGGAGATTTACAAGAGATTGGCAGCTTCTCGCCAATTGGCTGATCATGGATTGATAGTTGAAAGCCAAAGCAACTTCTTCAACTTGATGGATATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

17.36

Weight (kDa)

10.61

Isoelectric Point (pI)

48.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 60
AccB1I GGYRCC 1 cut(s) 60
AccII CGCG 1 cut(s) 284
AciI CCGC 4 cut(s) 114, 251, 264, 282
AclWI GGATC 1 cut(s) 315
AcsI RAATTY 1 cut(s) 182
AfaI GTAC 3 cut(s) 23, 44, 62
AfiI CCNNNNNNNGG 2 cut(s) 171, 310
AgsI TTSAA 3 cut(s) 37, 422, 442
AhlI ACTAGT 1 cut(s) 134
AjnI CCWGG 1 cut(s) 303
AluBI AGCT 6 cut(s) 70, 122, 157, 169, 202, 386
AluI AGCT 6 cut(s) 70, 122, 157, 169, 202, 386
Alw21I GWGCWC 1 cut(s) 134
AlwI GGATC 1 cut(s) 315
AlwNI CAGNNNCTG 1 cut(s) 323
AoxI GGCC 1 cut(s) 258
ApeKI GCWGC 2 cut(s) 323, 383
ApoI RAATTY 1 cut(s) 182
Asp718I GGTACC 1 cut(s) 60
AspS9I GGNCC 1 cut(s) 232
AsuHPI GGTGA 3 cut(s) 89, 183, 344
AvaII GGWCC 1 cut(s) 232
BanI GGYRCC 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 134
BbvCI CCTCAGC 1 cut(s) 170
BbvI GCAGC 2 cut(s) 310, 395
BccI CCATC 2 cut(s) 241, 442
BciT130I CCWGG 1 cut(s) 305
BclI TGATCA 2 cut(s) 334, 403
BcuI ACTAGT 1 cut(s) 134
BfaI CTAG 2 cut(s) 84, 135
BisI GCNGC 3 cut(s) 265, 324, 384
BlsI GCNGC 3 cut(s) 266, 325, 385
Bme1390I CCNGG 1 cut(s) 305
Bme18I GGWCC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 232
BmiI GGNNCC 1 cut(s) 62
BmrFI CCNGG 1 cut(s) 305
BpmI CTGGAG 1 cut(s) 67
Bpu10I CCTNAGC 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 143
BsaAI YACGTR 2 cut(s) 21, 229
BsaJI CCNNGG 1 cut(s) 303
BsaXI ACNNNNNCTCC 2 cut(s) 121, 151
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 310
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 1 cut(s) 50
BseBI CCWGG 1 cut(s) 305
BseDI CCNNGG 1 cut(s) 303
BseGI GGATG 1 cut(s) 252
BseLI CCNNNNNNNGG 2 cut(s) 171, 310
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 1 cut(s) 50
BseRI GAGGAG 3 cut(s) 64, 67, 132
BseXI GCAGC 2 cut(s) 310, 395
Bsh1236I CGCG 1 cut(s) 284
BshFI GGCC 1 cut(s) 260
BshNI GGYRCC 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 134
BsiSI CCGG 1 cut(s) 261
BslI CCNNNNNNNGG 2 cut(s) 171, 310
BsnI GGCC 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 134
Bsp143I GATC 3 cut(s) 307, 334, 403
BspACI CCGC 4 cut(s) 114, 251, 264, 282
BspANI GGCC 1 cut(s) 260
BspCNI CTCAG 1 cut(s) 162
BspFNI CGCG 1 cut(s) 284
BspLI GGNNCC 1 cut(s) 62
BspPI GGATC 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 1 cut(s) 50
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 1 cut(s) 303
BssMI GATC 3 cut(s) 307, 334, 403
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 224
Bst6I CTCTTC 1 cut(s) 32
BstBAI YACGTR 2 cut(s) 21, 229
BstC8I GCNNGC 1 cut(s) 262
BstDEI CTNAG 1 cut(s) 170
BstF5I GGATG 1 cut(s) 252
BstFNI CGCG 1 cut(s) 284
BstKTI GATC 3 cut(s) 310, 337, 406
BstMBI GATC 3 cut(s) 307, 334, 403
BstMWI GCNNNNNNNGC 1 cut(s) 257
BstNI CCWGG 1 cut(s) 305
BstSCI CCNGG 1 cut(s) 303
BstSNI TACGTA 1 cut(s) 21
BstUI CGCG 1 cut(s) 284
BstV1I GCAGC 2 cut(s) 310, 395
BsuRI GGCC 1 cut(s) 260
BtsCI GGATG 1 cut(s) 252
BtsIMutI CAGTG 1 cut(s) 324
Cac8I GCNNGC 1 cut(s) 262
CaiI CAGNNNCTG 1 cut(s) 323
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 1 cut(s) 232
Csp6I GTAC 3 cut(s) 22, 43, 61
CviAII CATG 1 cut(s) 407
CviQI GTAC 3 cut(s) 22, 43, 61
DdeI CTNAG 1 cut(s) 170
DpnI GATC 3 cut(s) 309, 336, 405
DpnII GATC 3 cut(s) 307, 334, 403
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 1 cut(s) 129
Eco105I TACGTA 1 cut(s) 21
Eco47I GGWCC 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 303
FaeI CATG 1 cut(s) 410
FaiI YATR 3 cut(s) 219, 408, 455
FatI CATG 1 cut(s) 406
FauI CCCGC 1 cut(s) 244
FbaI TGATCA 2 cut(s) 334, 403
Fnu4HI GCNGC 3 cut(s) 265, 324, 384
FokI GGATG 1 cut(s) 259
Fsp4HI GCNGC 3 cut(s) 265, 324, 384
FspBI CTAG 2 cut(s) 84, 135
GluI GCNGC 3 cut(s) 265, 324, 384
GsuI CTGGAG 1 cut(s) 67
HaeIII GGCC 1 cut(s) 260
HapII CCGG 1 cut(s) 261
Hin1II CATG 1 cut(s) 410
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 1 cut(s) 200
HinfI GANTC 1 cut(s) 241
HpaII CCGG 1 cut(s) 261
HphI GGTGA 3 cut(s) 89, 183, 344
Hpy166II GTNNAC 2 cut(s) 232, 346
Hpy188I TCNGA 2 cut(s) 15, 246
Hpy188III TCNNGA 1 cut(s) 160
Hpy8I GTNNAC 2 cut(s) 232, 346
HpyAV CCTTC 1 cut(s) 51
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4IV ACGT 2 cut(s) 20, 228
HpyCH4V TGCA 2 cut(s) 217, 296
HpyF10VI GCNNNNNNNGC 1 cut(s) 257
HpyF3I CTNAG 1 cut(s) 170
HpySE526I ACGT 2 cut(s) 20, 228
Hsp92II CATG 1 cut(s) 410
KpnI GGTACC 1 cut(s) 64
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
Ksp22I TGATCA 2 cut(s) 334, 403
Kzo9I GATC 3 cut(s) 307, 334, 403
LmnI GCTCC 2 cut(s) 119, 129
LpnPI CCDG 7 cut(s) 31, 248, 274, 290, 296, 317, 340
Lsp1109I GCAGC 2 cut(s) 310, 395
MaeI CTAG 2 cut(s) 84, 135
MaeII ACGT 2 cut(s) 20, 228
MaeIII GTNAC 2 cut(s) 100, 224
MalI GATC 3 cut(s) 309, 336, 405
MboI GATC 3 cut(s) 307, 334, 403
MboII GAAGA 5 cut(s) 49, 103, 298, 301, 430
MfeI CAATTG 1 cut(s) 395
MhlI GDGCHC 1 cut(s) 134
MluCI AATT 2 cut(s) 182, 395
MmeI TCCRAC 2 cut(s) 38, 131
MnlI CCTC 7 cut(s) 33, 42, 45, 90, 110, 165, 267
MroNI GCCGGC 1 cut(s) 260
MspI CCGG 1 cut(s) 261
MspR9I CCNGG 1 cut(s) 305
MunI CAATTG 1 cut(s) 395
MvaI CCWGG 1 cut(s) 305
MvnI CGCG 1 cut(s) 284
MwoI GCNNNNNNNGC 1 cut(s) 257
NaeI GCCGGC 1 cut(s) 262
NdeII GATC 3 cut(s) 307, 334, 403
NgoMIV GCCGGC 1 cut(s) 260
NlaIII CATG 1 cut(s) 410
NlaIV GGNNCC 1 cut(s) 62
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 1 cut(s) 100
PdiI GCCGGC 1 cut(s) 262
PfeI GAWTC 1 cut(s) 241
PkrI GCNGC 3 cut(s) 266, 325, 385
Ppu21I YACGTR 2 cut(s) 21, 229
Psp6I CCWGG 1 cut(s) 303
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 62
PspPI GGNCC 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 323
RsaI GTAC 3 cut(s) 23, 44, 62
RsaNI GTAC 3 cut(s) 22, 43, 61
SatI GCNGC 3 cut(s) 265, 324, 384
Sau3AI GATC 3 cut(s) 307, 334, 403
Sau96I GGNCC 1 cut(s) 232
ScrFI CCNGG 1 cut(s) 305
SduI GDGCHC 1 cut(s) 134
SinI GGWCC 1 cut(s) 232
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
SnaBI TACGTA 1 cut(s) 21
SpeI ACTAGT 1 cut(s) 134
Sse9I AATT 2 cut(s) 182, 395
SsiI CCGC 4 cut(s) 114, 251, 264, 282
SspMI CTAG 2 cut(s) 84, 135
StyD4I CCNGG 1 cut(s) 303
TaaI ACNGT 1 cut(s) 224
TaiI ACGT 2 cut(s) 23, 231
TasI AATT 2 cut(s) 182, 395
TauI GCSGC 1 cut(s) 267
TfiI GAWTC 1 cut(s) 241
TscAI CASTG 1 cut(s) 324
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 2 cut(s) 323, 383
Tsp45I GTSAC 1 cut(s) 100
TspDTI ATGAA 1 cut(s) 194
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 1 cut(s) 324
VpaK11BI GGWCC 1 cut(s) 232
XapI RAATTY 1 cut(s) 182
XcmI CCANNNNNNNNNTGG 1 cut(s) 71
XspI CTAG 2 cut(s) 84, 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.