Rh4BG338200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001685
Physical Location & Seq
Reverse (-)
50099914 .. 50100797
884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG338200.1

Sequence Viewer

Length: 408 bp
ATGGAGATTGCTCCAAGCTCGTACTACAACAACATGAAGAGATACTGGAGGAGGAGGAGGAGGTACCAAAGGCTGAGCGTCGACAACAAGAAGAAGCTGAGAGTTCAAGGGCTCGGCGGAGGCCCTAATGGGACTAGTCCTCCTCGGCGGTTTTTCAAGATCAGAAAGCTTAGGGTCCTCAAGTTCGTTTCCCCTATAACTCTCTTGGCCAAGTTCCATGGTGCTTATGTTGACATGATGGTCAAGATGGCGGGAAACGTTGGCGGCGCCAGAAGGATTGCAGGAGGATCAAAGAAGGTGGCAAAGGCACAAGATCAGATTTCGGTGGCAGCTTCTTGCAGCGAAGAAGCCGTTGACACCAAATTGGTTTTGGAGATTTACAAGAGATTGGCGGCTTCTCGGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

15.26

Weight (kDa)

11.05

Isoelectric Point (pI)

61.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
Acc65I GGTACC 1 cut(s) 63
AccB1I GGYRCC 2 cut(s) 63, 266
AccI GTMKAC 1 cut(s) 81
AciI CCGC 5 cut(s) 117, 148, 251, 264, 392
AclI AACGTT 1 cut(s) 258
AclWI GGATC 1 cut(s) 295
AcoI YGGCCR 1 cut(s) 207
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 2 cut(s) 23, 65
AgsI TTSAA 2 cut(s) 107, 157
AhlI ACTAGT 1 cut(s) 134
AluBI AGCT 4 cut(s) 18, 97, 169, 332
AluI AGCT 4 cut(s) 18, 97, 169, 332
AlwI GGATC 1 cut(s) 295
AoxI GGCC 2 cut(s) 121, 207
ApeKI GCWGC 2 cut(s) 329, 339
Asp718I GGTACC 1 cut(s) 63
AspLEI GCGC 1 cut(s) 269
AspS9I GGNCC 2 cut(s) 122, 175
AvaII GGWCC 1 cut(s) 175
BalI TGGCCA 1 cut(s) 209
BanI GGYRCC 2 cut(s) 63, 266
BanII GRGCYC 1 cut(s) 114
BbvI GCAGC 2 cut(s) 341, 351
BccI CCATC 2 cut(s) 232, 241
BceAI ACGGC 1 cut(s) 335
BcuI ACTAGT 1 cut(s) 134
BfaI CTAG 1 cut(s) 135
BfoI RGCGCY 1 cut(s) 270
BisI GCNGC 4 cut(s) 265, 330, 340, 393
BlpI GCTNAGC 1 cut(s) 74
BlsI GCNGC 4 cut(s) 266, 331, 341, 394
Bme18I GGWCC 1 cut(s) 175
BmgT120I GGNCC 2 cut(s) 122, 175
BmiI GGNNCC 3 cut(s) 65, 176, 268
BpmI CTGGAG 1 cut(s) 67
Bpu10I CCTNAGC 1 cut(s) 170
Bpu1102I GCTNAGC 1 cut(s) 74
BpuEI CTTGAG 1 cut(s) 164
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 2 cut(s) 143, 217
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bse1I ACTGG 1 cut(s) 50
BseDI CCNNGG 2 cut(s) 143, 217
BseMII CTCAG 2 cut(s) 65, 89
BseNI ACTGG 1 cut(s) 50
BseRI GAGGAG 5 cut(s) 64, 67, 70, 73, 132
BseXI GCAGC 2 cut(s) 341, 351
BshFI GGCC 2 cut(s) 123, 209
BshNI GGYRCC 2 cut(s) 63, 266
BslFI GGGAC 1 cut(s) 145
BsmFI GGGAC 1 cut(s) 145
BsnI GGCC 2 cut(s) 123, 209
Bsp1286I GDGCHC 1 cut(s) 114
Bsp143I GATC 3 cut(s) 159, 287, 313
Bsp1720I GCTNAGC 1 cut(s) 74
Bsp19I CCATGG 1 cut(s) 217
BspACI CCGC 5 cut(s) 117, 148, 251, 264, 392
BspANI GGCC 2 cut(s) 123, 209
BspCNI CTCAG 2 cut(s) 66, 90
BspLI GGNNCC 3 cut(s) 65, 176, 268
BspPI GGATC 1 cut(s) 295
BspT107I GGYRCC 2 cut(s) 63, 266
BsrI ACTGG 1 cut(s) 50
BssECI CCNNGG 2 cut(s) 143, 217
BssMI GATC 3 cut(s) 159, 287, 313
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 217
Bst6I CTCTTC 1 cut(s) 32
BstACI GRCGYC 1 cut(s) 267
BstDEI CTNAG 3 cut(s) 74, 98, 170
BstDSI CCRYGG 1 cut(s) 217
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 3 cut(s) 162, 290, 316
BstMBI GATC 3 cut(s) 159, 287, 313
BstV1I GCAGC 2 cut(s) 341, 351
BsuRI GGCC 2 cut(s) 123, 209
BtgI CCRYGG 1 cut(s) 217
CfoI GCGC 1 cut(s) 269
Cfr13I GGNCC 2 cut(s) 122, 175
CseI GACGC 1 cut(s) 67
Csp6I GTAC 2 cut(s) 22, 64
CspCI CAANNNNNGTGG 2 cut(s) 279, 314
CviAII CATG 3 cut(s) 34, 218, 235
CviQI GTAC 2 cut(s) 22, 64
DdeI CTNAG 3 cut(s) 74, 98, 170
DinI GGCGCC 1 cut(s) 268
DpnI GATC 3 cut(s) 161, 289, 315
DpnII GATC 3 cut(s) 159, 287, 313
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
EaeI YGGCCR 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 1 cut(s) 132
Eco130I CCWWGG 1 cut(s) 217
Eco24I GRGCYC 1 cut(s) 114
Eco47I GGWCC 1 cut(s) 175
EcoO109I RGGNCCY 2 cut(s) 122, 175
EcoT14I CCWWGG 1 cut(s) 217
EcoT38I GRGCYC 1 cut(s) 114
EgeI GGCGCC 1 cut(s) 268
EheI GGCGCC 1 cut(s) 268
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 3 cut(s) 37, 221, 238
FaiI YATR 5 cut(s) 35, 197, 219, 228, 236
FaqI GGGAC 1 cut(s) 145
FatI CATG 3 cut(s) 33, 217, 234
FauI CCCGC 1 cut(s) 244
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 4 cut(s) 265, 330, 340, 393
FriOI GRGCYC 1 cut(s) 114
Fsp4HI GCNGC 4 cut(s) 265, 330, 340, 393
FspBI CTAG 1 cut(s) 135
GlaI GCGC 1 cut(s) 268
GluI GCNGC 4 cut(s) 265, 330, 340, 393
GsuI CTGGAG 1 cut(s) 67
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 2 cut(s) 123, 209
HgaI GACGC 1 cut(s) 67
HhaI GCGC 1 cut(s) 269
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 3 cut(s) 37, 221, 238
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
HincII GTYRAC 3 cut(s) 82, 232, 355
HindII GTYRAC 3 cut(s) 82, 232, 355
HindIII AAGCTT 1 cut(s) 167
Hpy166II GTNNAC 3 cut(s) 82, 232, 355
Hpy188I TCNGA 2 cut(s) 164, 318
Hpy188III TCNNGA 2 cut(s) 157, 244
Hpy8I GTNNAC 3 cut(s) 82, 232, 355
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 2 cut(s) 267, 289
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 2 cut(s) 281, 339
HpyF3I CTNAG 3 cut(s) 74, 98, 170
HpySE526I ACGT 1 cut(s) 258
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 3 cut(s) 37, 221, 238
HspAI GCGC 1 cut(s) 267
KasI GGCGCC 1 cut(s) 266
KpnI GGTACC 1 cut(s) 67
Kzo9I GATC 3 cut(s) 159, 287, 313
LmnI GCTCC 1 cut(s) 16
LpnPI CCDG 3 cut(s) 31, 267, 283
Lsp1109I GCAGC 2 cut(s) 341, 351
MaeI CTAG 1 cut(s) 135
MaeII ACGT 1 cut(s) 258
MalI GATC 3 cut(s) 161, 289, 315
MboI GATC 3 cut(s) 159, 287, 313
MboII GAAGA 3 cut(s) 49, 103, 356
MhlI GDGCHC 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 1 cut(s) 362
MluNI TGGCCA 1 cut(s) 209
Mly113I GGCGCC 1 cut(s) 267
Mox20I TGGCCA 1 cut(s) 209
MscI TGGCCA 1 cut(s) 209
Msp20I TGGCCA 1 cut(s) 209
NarI GGCGCC 1 cut(s) 267
NcoI CCATGG 1 cut(s) 217
NdeII GATC 3 cut(s) 159, 287, 313
NlaIII CATG 3 cut(s) 37, 221, 238
NlaIV GGNNCC 3 cut(s) 65, 176, 268
NmeAIII GCCGAG 3 cut(s) 93, 124, 379
PkrI GCNGC 4 cut(s) 266, 331, 341, 394
PluTI GGCGCC 1 cut(s) 270
PpuMI RGGWCCY 1 cut(s) 175
Psp1406I AACGTT 1 cut(s) 258
Psp5II RGGWCCY 1 cut(s) 175
PspN4I GGNNCC 3 cut(s) 65, 176, 268
PspPI GGNCC 2 cut(s) 122, 175
PspPPI RGGWCCY 1 cut(s) 175
RsaI GTAC 2 cut(s) 23, 65
RsaNI GTAC 2 cut(s) 22, 64
SalI GTCGAC 1 cut(s) 80
SatI GCNGC 4 cut(s) 265, 330, 340, 393
Sau3AI GATC 3 cut(s) 159, 287, 313
Sau96I GGNCC 2 cut(s) 122, 175
SduI GDGCHC 1 cut(s) 114
SetI ASST 7 cut(s) 20, 65, 99, 171, 261, 300, 334
SfoI GGCGCC 1 cut(s) 268
SinI GGWCC 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
SpeI ACTAGT 1 cut(s) 134
Sse9I AATT 1 cut(s) 362
SsiI CCGC 5 cut(s) 117, 148, 251, 264, 392
SspDI GGCGCC 1 cut(s) 266
SspMI CTAG 1 cut(s) 135
StyI CCWWGG 1 cut(s) 217
TaiI ACGT 1 cut(s) 261
TaqI TCGA 1 cut(s) 81
TasI AATT 1 cut(s) 362
TauI GCSGC 2 cut(s) 267, 395
TseI GCWGC 2 cut(s) 329, 339
TspDTI ATGAA 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 175
XcmI CCANNNNNNNNNTGG 1 cut(s) 367
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.