pycom16g06270

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005271
Physical Location & Seq
Reverse (-)
4009923 .. 4010900
978 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 708 bp
ATGGATTCGAAATTCAACCGTTCGATGTTCATCAGGCTCTTCATAATACAGTGTCTAGCAGTTGTTTCTGTTTCTCAGAGTTTTGATTTTTTCTATTTTGTACAGCAGTGGCCGGGATCTTACTGTGACACCGAGGCAAGTTGTTGCTATCCAACGACAGGAAAACCTGCACGAGAATTCGGCATCCATGGGCTTTGGCCTAATTACAATGACGGTTCTTATCCATCCAACTGTGATTCTGACAACCCTTATGATGAATCGAAGGTAGTAATAATTAGAACTAGGATCTCGGACCTGATAAGCAGACTGCAAGCAGATTGGCCTACACTGGCATGCCCCAGCGGGAATGGCTCAAAGTTTTGGGCACATGAGTGGAATAAACATGGGACATGCTCCGAGTCCGTGCTTGACCAACATGGCTACTTTGAGGCCTCTCTCAACCTCAAGGAAAACTTAGACATCTTCCAAATCCTTCAAAATGCTGGAATCCAAGCAGATGGGAATATTTACAGCTTGAGCAGCATAAAAGAAGCTATAAAATCAGCAATTGGGTACACTCCTGGTATAACATGTAACGAAGATGCATCCGGGAATAGCCAGCTTTATGAGGTTTATATTTGTGTGGACACATCTGGCTCAAACCTTATTGAATGTCCAGTGCTGCCCGGGACGAAATGCTCCTCCAAAATTGAGTTCCCTTCCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

236

Amino Acids

26.17

Weight (kDa)

4.76

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 28 - 218 8.7e-62 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 175
AciI CCGC 1 cut(s) 342
AclWI GGATC 2 cut(s) 124, 293
AcoI YGGCCR 1 cut(s) 110
AcsI RAATTY 2 cut(s) 11, 176
AfaI GTAC 2 cut(s) 102, 554
AfiI CCNNNNNNNGG 1 cut(s) 158
AflIII ACRYGT 1 cut(s) 569
AgsI TTSAA 3 cut(s) 16, 476, 650
AjnI CCWGG 1 cut(s) 559
AjuI GAANNNNNNNTTGG 1 cut(s) 677
AleI CACNNNNGTG 1 cut(s) 370
AluBI AGCT 3 cut(s) 513, 533, 601
AluI AGCT 3 cut(s) 513, 533, 601
AlwI GGATC 2 cut(s) 124, 293
Ama87I CYCGRG 1 cut(s) 665
AoxI GGCC 4 cut(s) 110, 197, 320, 429
ApeKI GCWGC 2 cut(s) 519, 661
ApoI RAATTY 2 cut(s) 11, 176
AspS9I GGNCC 1 cut(s) 292
AsuC2I CCSGG 4 cut(s) 114, 589, 666, 667
AsuII TTCGAA 1 cut(s) 8
AvaI CYCGRG 1 cut(s) 665
AvaII GGWCC 1 cut(s) 292
BaeGI GKGCMC 1 cut(s) 367
BauI CACGAG 1 cut(s) 171
BbvI GCAGC 2 cut(s) 531, 648
BccI CCATC 2 cut(s) 232, 491
BciT130I CCWGG 1 cut(s) 561
BcnI CCSGG 4 cut(s) 114, 589, 666, 667
BfaI CTAG 3 cut(s) 56, 282, 706
BfuAI ACCTGC 1 cut(s) 175
BisI GCNGC 2 cut(s) 520, 662
BlsI GCNGC 2 cut(s) 521, 663
Bme1390I CCNGG 5 cut(s) 114, 561, 589, 666, 667
Bme18I GGWCC 1 cut(s) 292
BmeT110I CYCGRG 1 cut(s) 665
BmgT120I GGNCC 1 cut(s) 292
BmrFI CCNGG 5 cut(s) 114, 561, 589, 666, 667
BmsI GCATC 3 cut(s) 192, 571, 593
Bpu14I TTCGAA 1 cut(s) 8
BpuEI CTTGAG 2 cut(s) 428, 535
BpuMI CCSGG 4 cut(s) 114, 589, 666, 667
BsaBI GATNNNNATC 1 cut(s) 29
BsaJI CCNNGG 3 cut(s) 132, 187, 665
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse1I ACTGG 2 cut(s) 333, 656
Bse8I GATNNNNATC 1 cut(s) 29
BseBI CCWGG 1 cut(s) 561
BseDI CCNNGG 3 cut(s) 132, 187, 665
BseGI GGATG 3 cut(s) 183, 224, 584
BseJI GATNNNNATC 1 cut(s) 29
BseLI CCNNNNNNNGG 1 cut(s) 158
BseMII CTCAG 1 cut(s) 89
BseNI ACTGG 2 cut(s) 333, 656
BseRI GAGGAG 1 cut(s) 670
BseSI GKGCMC 1 cut(s) 367
BseXI GCAGC 2 cut(s) 531, 648
BseYI CCCAGC 1 cut(s) 338
BsgI GTGCAG 1 cut(s) 153
BshFI GGCC 4 cut(s) 112, 199, 322, 431
BsiHKCI CYCGRG 1 cut(s) 665
BsiSI CCGG 3 cut(s) 113, 588, 666
BslFI GGGAC 2 cut(s) 400, 682
BslI CCNNNNNNNGG 1 cut(s) 158
BsmFI GGGAC 2 cut(s) 400, 682
BsnI GGCC 4 cut(s) 112, 199, 322, 431
BsoBI CYCGRG 1 cut(s) 665
Bsp119I TTCGAA 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 367
Bsp1407I TGTACA 1 cut(s) 100
Bsp143I GATC 2 cut(s) 116, 285
Bsp19I CCATGG 1 cut(s) 187
BspACI CCGC 1 cut(s) 342
BspANI GGCC 4 cut(s) 112, 199, 322, 431
BspCNI CTCAG 1 cut(s) 88
BspMI ACCTGC 1 cut(s) 175
BspPI GGATC 2 cut(s) 124, 293
BspQI GCTCTTC 1 cut(s) 44
BspT104I TTCGAA 1 cut(s) 8
BsrGI TGTACA 1 cut(s) 100
BsrI ACTGG 2 cut(s) 333, 656
BssECI CCNNGG 3 cut(s) 132, 187, 665
BssMI GATC 2 cut(s) 116, 285
BssSI CACGAG 1 cut(s) 171
BssT1I CCWWGG 1 cut(s) 187
Bst2BI CACGAG 1 cut(s) 171
Bst2UI CCWGG 1 cut(s) 561
Bst4CI ACNGT 5 cut(s) 20, 51, 125, 215, 233
Bst6I CTCTTC 1 cut(s) 44
BstAUI TGTACA 1 cut(s) 100
BstBI TTCGAA 1 cut(s) 8
BstC8I GCNNGC 3 cut(s) 312, 334, 599
BstDEI CTNAG 2 cut(s) 75, 454
BstDSI CCRYGG 1 cut(s) 187
BstF5I GGATG 3 cut(s) 183, 224, 584
BstKTI GATC 2 cut(s) 119, 288
BstMBI GATC 2 cut(s) 116, 285
BstMWI GCNNNNNNNGC 2 cut(s) 348, 519
BstNI CCWGG 1 cut(s) 561
BstNSI RCATGY 3 cut(s) 336, 393, 573
BstSCI CCNGG 5 cut(s) 112, 559, 587, 664, 665
BstSLI GKGCMC 1 cut(s) 367
BstV1I GCAGC 2 cut(s) 531, 648
BstX2I RGATCY 2 cut(s) 116, 285
BstXI CCANNNNNNTGG 1 cut(s) 497
BstYI RGATCY 2 cut(s) 116, 285
BsuRI GGCC 4 cut(s) 112, 199, 322, 431
BtgI CCRYGG 1 cut(s) 187
BtsCI GGATG 3 cut(s) 183, 224, 584
BtsI GCAGTG 1 cut(s) 113
BtsIMutI CAGTG 4 cut(s) 56, 113, 326, 663
BveI ACCTGC 1 cut(s) 175
Cac8I GCNNGC 3 cut(s) 312, 334, 599
Cfr13I GGNCC 1 cut(s) 292
Cfr9I CCCGGG 1 cut(s) 665
Csp6I GTAC 2 cut(s) 101, 553
CviAII CATG 7 cut(s) 188, 333, 368, 383, 390, 416, 570
CviQI GTAC 2 cut(s) 101, 553
DdeI CTNAG 2 cut(s) 75, 454
DpnI GATC 2 cut(s) 118, 287
DpnII GATC 2 cut(s) 116, 285
EaeI YGGCCR 1 cut(s) 110
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 187
Eco147I AGGCCT 1 cut(s) 431
Eco47I GGWCC 1 cut(s) 292
Eco88I CYCGRG 1 cut(s) 665
EcoRI GAATTC 1 cut(s) 176
EcoRII CCWGG 1 cut(s) 559
EcoT14I CCWWGG 1 cut(s) 187
EcoT22I ATGCAT 1 cut(s) 586
ErhI CCWWGG 1 cut(s) 187
FaeI CATG 7 cut(s) 191, 336, 371, 386, 393, 419, 573
FalI AAGNNNNNCTT 2 cut(s) 437, 469
FaqI GGGAC 2 cut(s) 400, 682
FatI CATG 7 cut(s) 187, 332, 367, 382, 389, 415, 569
FauI CCCGC 1 cut(s) 335
Fnu4HI GCNGC 2 cut(s) 520, 662
FokI GGATG 3 cut(s) 170, 211, 571
Fsp4HI GCNGC 2 cut(s) 520, 662
FspBI CTAG 3 cut(s) 56, 282, 706
GluI GCNGC 2 cut(s) 520, 662
GsaI CCCAGC 1 cut(s) 342
HaeIII GGCC 4 cut(s) 112, 199, 322, 431
HapII CCGG 3 cut(s) 113, 588, 666
Hin1II CATG 7 cut(s) 191, 336, 371, 386, 393, 419, 573
HinfI GANTC 5 cut(s) 5, 236, 257, 398, 486
HpaII CCGG 3 cut(s) 113, 588, 666
Hpy166II GTNNAC 2 cut(s) 555, 625
Hpy188I TCNGA 4 cut(s) 78, 241, 292, 397
Hpy8I GTNNAC 2 cut(s) 555, 625
HpyAV CCTTC 3 cut(s) 256, 482, 708
HpyCH4III ACNGT 5 cut(s) 20, 51, 125, 215, 233
HpyCH4V TGCA 3 cut(s) 170, 310, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 348, 519
HpyF3I CTNAG 2 cut(s) 75, 454
Hsp92II CATG 7 cut(s) 191, 336, 371, 386, 393, 419, 573
Kzo9I GATC 2 cut(s) 116, 285
LguI GCTCTTC 1 cut(s) 44
LmnI GCTCC 2 cut(s) 398, 683
Lsp1109I GCAGC 2 cut(s) 531, 648
LweI GCATC 3 cut(s) 192, 571, 593
MaeI CTAG 3 cut(s) 56, 282, 706
MaeIII GTNAC 2 cut(s) 125, 572
MalI GATC 2 cut(s) 118, 287
MboI GATC 2 cut(s) 116, 285
MboII GAAGA 3 cut(s) 31, 454, 590
MfeI CAATTG 1 cut(s) 546
MflI RGATCY 2 cut(s) 116, 285
MhlI GDGCHC 1 cut(s) 367
MluCI AATT 6 cut(s) 11, 176, 202, 273, 546, 687
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 2 cut(s) 176, 252
MnlI CCTC 6 cut(s) 127, 421, 442, 452, 601, 691
Mph1103I ATGCAT 1 cut(s) 586
MslI CAYNNNNRTG 2 cut(s) 331, 370
MspA1I CMGCKG 1 cut(s) 342
MspI CCGG 3 cut(s) 113, 588, 666
MspR9I CCNGG 5 cut(s) 114, 561, 589, 666, 667
MunI CAATTG 1 cut(s) 546
MvaI CCWGG 1 cut(s) 561
MwoI GCNNNNNNNGC 2 cut(s) 348, 519
NciI CCSGG 4 cut(s) 114, 589, 666, 667
NcoI CCATGG 1 cut(s) 187
NdeII GATC 2 cut(s) 116, 285
NlaIII CATG 7 cut(s) 191, 336, 371, 386, 393, 419, 573
NmuCI GTSAC 1 cut(s) 125
NsiI ATGCAT 1 cut(s) 586
NspI RCATGY 3 cut(s) 336, 393, 573
NspV TTCGAA 1 cut(s) 8
OliI CACNNNNGTG 1 cut(s) 370
PaeI GCATGC 1 cut(s) 336
PceI AGGCCT 1 cut(s) 431
PciI ACATGT 1 cut(s) 569
PciSI GCTCTTC 1 cut(s) 44
PfeI GAWTC 4 cut(s) 5, 236, 257, 486
PfoI TCCNGGA 1 cut(s) 587
PkrI GCNGC 2 cut(s) 521, 663
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
PscI ACATGT 1 cut(s) 569
Psp6I CCWGG 1 cut(s) 559
PspFI CCCAGC 1 cut(s) 338
PspGI CCWGG 1 cut(s) 559
PspPI GGNCC 1 cut(s) 292
PsuI RGATCY 2 cut(s) 116, 285
RsaI GTAC 2 cut(s) 102, 554
RsaNI GTAC 2 cut(s) 101, 553
RseI CAYNNNNRTG 2 cut(s) 331, 370
SapI GCTCTTC 1 cut(s) 44
SatI GCNGC 2 cut(s) 520, 662
Sau3AI GATC 2 cut(s) 116, 285
Sau96I GGNCC 1 cut(s) 292
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 5 cut(s) 114, 561, 589, 666, 667
SduI GDGCHC 1 cut(s) 367
SetI ASST 9 cut(s) 169, 267, 297, 444, 515, 535, 603, 612, 645
SfaNI GCATC 3 cut(s) 192, 571, 593
SfuI TTCGAA 1 cut(s) 8
SinI GGWCC 1 cut(s) 292
SmaI CCCGGG 1 cut(s) 667
SmiMI CAYNNNNRTG 2 cut(s) 331, 370
SmlI CTYRAG 2 cut(s) 443, 514
SmoI CTYRAG 2 cut(s) 443, 514
SphI GCATGC 1 cut(s) 336
Sse9I AATT 6 cut(s) 11, 176, 202, 273, 546, 687
SseBI AGGCCT 1 cut(s) 431
SsiI CCGC 1 cut(s) 342
SspI AATATT 1 cut(s) 505
SspMI CTAG 3 cut(s) 56, 282, 706
StuI AGGCCT 1 cut(s) 431
StyD4I CCNGG 5 cut(s) 112, 559, 587, 664, 665
StyI CCWWGG 1 cut(s) 187
TaaI ACNGT 5 cut(s) 20, 51, 125, 215, 233
TaqI TCGA 3 cut(s) 8, 23, 260
TasI AATT 6 cut(s) 11, 176, 202, 273, 546, 687
TatI WGTACW 1 cut(s) 100
TfiI GAWTC 4 cut(s) 5, 236, 257, 486
TscAI CASTG 4 cut(s) 56, 113, 333, 663
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 2 cut(s) 519, 661
Tsp45I GTSAC 1 cut(s) 125
TspDTI ATGAA 3 cut(s) 19, 31, 270
TspGWI ACGGA 1 cut(s) 391
TspMI CCCGGG 1 cut(s) 665
TspRI CASTG 4 cut(s) 56, 113, 333, 663
VpaK11BI GGWCC 1 cut(s) 292
XapI RAATTY 2 cut(s) 11, 176
XceI RCATGY 3 cut(s) 336, 393, 573
XmaI CCCGGG 1 cut(s) 665
XspI CTAG 3 cut(s) 56, 282, 706
Zsp2I ATGCAT 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.