pycom16g07000

tetratricopeptide repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004639
Physical Location & Seq
Reverse (-)
4546437 .. 4550268
3832 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g07000.11

Sequence Viewer

Length: 1410 bp
ATGAAGGGAGAGTTAAAAGTGGACAAGTTTGGATACGAGGTGAGGACCTCTTCAGACGCCTGCATCTCCGCCATCAACGCCTTCTACGACCAGGTTTTGAATTATGGCCGGCGGCGGTCAGTGATATTGGAGGCGGCAGCGCACGACGGCCAGTGCGTGCTTGCTAACATACTGGCTGCCCATTTCCTCTCCTCCTCCGATCCTTCTCGTGCTCCTCCTCATCTCCAAGCCGCCAAGTCCCAGCTGGAACAAGCTACCCCGTACGAGAAAGCAGTGTTTGCTGCTGTCAATAGTTTGATTTCTGTGGATAGAGACGATGATGTTGCTTTTGAGTTGCATTCAAAGCTCTTGAAATGTTTCCCTAAAGATTTGGCTTCTCTGAAAAGGGCCCAAGTGCTCTGCTTCTACAGTGCTCGACCTGATCTATCGTTGGATCTTGTTCAACAGGTTCTTCCCAGTAATGAAAAAGAAGATTACGTGTACGGCATGCTTGCTTTTCCTTTATTAGAGCTTGGACGAATGGAAGAGGCTGAGAAAGCTGCAAAAAAGGGATACGAGATCAACAAGCATGACAACTGGGTGCAACATAATATGTGCCATGTTCTTCAGTATAACTGCCACTTTAAAGAAGCAGTAGAGTTCATGGAAGAATGTTCACCTTCATGGGATTTGTGTTCGTCATTCATGCTTACACACAATTGGTGGCACGTAGCTCTTTGTTATTTAGAAGGCCATGCTCCAATCCAAAGAATCCGAGATTTATACGACCATCGAATCTGGAAGGAGTTGGAGAAACCTGATGCTGCACGTCCAGAGGTCTACTTGAATGCCCTTGGGTTGTTGTTACGGGTGCATGTTCGAGGTGAAATGGATGCCTTTGAGGACCATTTGAAGACTTTAGCTAATTGTGTGACAGATCAAGCCAACTGGTATTTAGAGTGGCATCTTGACGTGTTAATTTTATGGGCGTTGACTTGTACTGGTGAAATTTCAAAGGCAGAAGAATTACTTAAGGGCTTAAAATCCAGAATTGCGAAGATGAACAAAAAGAAGCAACAGTTCATGCAGAAAGCAATGCTGCTTGCAGAAGCCACGTACGAGTATGGGAAGGGTAATGAAAAACAAGCATTAGAATTGCTTGGTCCTGAATTTGATGCAGATAACTGCAAGATGATCGGAGCATCGGGTGAACAGCTTGATATATTCAATGAAGTCTGGTACTGTATGCTGCTTAACAATGGACAAGCTGCAAAAGCGATTGAAGTGCTTGAGAAGCGGATCAAAACAAGAGAGGGTATCCCCTTCTTTTGGCGCCTGCTGGAGAGAGGATATAAACTGGCGGGCAGGGAAGAGGAGGCAACAACTGCAAGTGAGGAAGCCAAGCGTTTGGAGAACGCATATTTCCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

470

Amino Acids

53.66

Weight (kDa)

5.68

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1311
AccI GTMKAC 1 cut(s) 819
AciI CCGC 7 cut(s) 69, 112, 115, 134, 231, 1276, 1340
AclWI GGATC 3 cut(s) 194, 441, 1286
AcoI YGGCCR 2 cut(s) 106, 148
AcsI RAATTY 2 cut(s) 987, 1148
AcuI CTGAAG 2 cut(s) 36, 590
AcyI GRCGYC 2 cut(s) 57, 1312
AfaI GTAC 5 cut(s) 263, 482, 979, 1097, 1220
AfiI CCNNNNNNNGG 1 cut(s) 1308
AflII CTTAAG 1 cut(s) 1010
AflIII ACRYGT 2 cut(s) 477, 951
AgsI TTSAA 9 cut(s) 100, 342, 352, 443, 826, 892, 993, 1207, 1262
AjiI CACGTC 2 cut(s) 809, 952
AjnI CCWGG 1 cut(s) 90
AluBI AGCT 9 cut(s) 244, 254, 346, 511, 539, 713, 902, 1195, 1247
AluI AGCT 9 cut(s) 244, 254, 346, 511, 539, 713, 902, 1195, 1247
Alw21I GWGCWC 3 cut(s) 214, 399, 415
Alw26I GTCTC 1 cut(s) 306
AlwI GGATC 3 cut(s) 194, 441, 1286
AoxI GGCC 4 cut(s) 106, 148, 387, 730
ApaI GGGCCC 1 cut(s) 391
ApeKI GCWGC 8 cut(s) 137, 176, 281, 539, 803, 1078, 1228, 1247
ApoI RAATTY 2 cut(s) 987, 1148
Asp700I GAANNNNTTC 1 cut(s) 356
AspLEI GCGC 2 cut(s) 142, 1314
AspS9I GGNCC 5 cut(s) 45, 387, 388, 883, 1142
AsuHPI GGTGA 5 cut(s) 52, 648, 875, 995, 1199
AvaII GGWCC 3 cut(s) 45, 883, 1142
BaeGI GKGCMC 1 cut(s) 391
BanI GGYRCC 1 cut(s) 1311
BanII GRGCYC 1 cut(s) 391
BauI CACGAG 1 cut(s) 207
BbsI GAAGAC 1 cut(s) 899
Bbv12I GWGCWC 3 cut(s) 214, 399, 415
BbvI GCAGC 8 cut(s) 149, 163, 268, 526, 790, 1065, 1215, 1234
BccI CCATC 2 cut(s) 80, 777
BceAI ACGGC 2 cut(s) 163, 499
BcgI CGANNNNNNTGC 6 cut(s) 305, 339, 1156, 1190, 1246, 1280
BciT130I CCWGG 1 cut(s) 92
BciVI GTATCC 3 cut(s) 26, 545, 1307
BcoDI GTCTC 1 cut(s) 306
BfmI CTRYAG 1 cut(s) 406
BfoI RGCGCY 1 cut(s) 1315
BfrI CTTAAG 1 cut(s) 1010
BfuI GTATCC 3 cut(s) 26, 545, 1307
Bme1390I CCNGG 1 cut(s) 92
Bme18I GGWCC 3 cut(s) 45, 883, 1142
BmgBI CACGTC 2 cut(s) 809, 952
BmgT120I GGNCC 5 cut(s) 45, 387, 388, 883, 1142
BmiI GGNNCC 2 cut(s) 389, 1313
BmrFI CCNGG 1 cut(s) 92
BmrI ACTGGG 2 cut(s) 450, 586
BmsI GCATC 6 cut(s) 72, 790, 862, 952, 1144, 1190
BmuI ACTGGG 2 cut(s) 450, 586
BpiI GAAGAC 1 cut(s) 899
BpmI CTGGAG 1 cut(s) 1340
BpuEI CTTGAG 1 cut(s) 1289
BsaAI YACGTR 3 cut(s) 478, 709, 1095
BsaHI GRCGYC 2 cut(s) 57, 1312
BsaJI CCNNGG 1 cut(s) 832
Bsc4I CCNNNNNNNGG 1 cut(s) 1308
Bse118I RCCGGY 1 cut(s) 108
Bse1I ACTGG 7 cut(s) 151, 177, 456, 581, 932, 985, 1341
Bse3DI GCAATG 1 cut(s) 1080
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 1 cut(s) 832
BseGI GGATG 1 cut(s) 877
BseLI CCNNNNNNNGG 1 cut(s) 1308
BseMI GCAATG 1 cut(s) 1080
BseMII CTCAG 1 cut(s) 522
BseNI ACTGG 7 cut(s) 151, 177, 456, 581, 932, 985, 1341
BseRI GAGGAG 5 cut(s) 181, 184, 204, 207, 1367
BseSI GKGCMC 1 cut(s) 391
BseXI GCAGC 8 cut(s) 149, 163, 268, 526, 790, 1065, 1215, 1234
BseYI CCCAGC 1 cut(s) 240
BsgI GTGCAG 1 cut(s) 789
BshFI GGCC 4 cut(s) 108, 150, 389, 732
BshNI GGYRCC 1 cut(s) 1311
BsiHKAI GWGCWC 3 cut(s) 214, 399, 415
BsiSI CCGG 1 cut(s) 109
BsiWI CGTACG 2 cut(s) 261, 1095
BslFI GGGAC 1 cut(s) 223
BslI CCNNNNNNNGG 1 cut(s) 1308
BsmAI GTCTC 1 cut(s) 306
BsmBI CGTCTC 1 cut(s) 306
BsmFI GGGAC 1 cut(s) 223
BsmI GAATGC 2 cut(s) 337, 832
BsnI GGCC 4 cut(s) 108, 150, 389, 732
Bsp120I GGGCCC 1 cut(s) 387
Bsp1286I GDGCHC 4 cut(s) 214, 391, 399, 415
Bsp143I GATC 7 cut(s) 199, 421, 433, 558, 916, 1173, 1278
BspACI CCGC 7 cut(s) 69, 112, 115, 134, 231, 1276, 1340
BspANI GGCC 4 cut(s) 108, 150, 389, 732
BspCNI CTCAG 1 cut(s) 523
BspLI GGNNCC 2 cut(s) 389, 1313
BspPI GGATC 3 cut(s) 194, 441, 1286
BspT107I GGYRCC 1 cut(s) 1311
BspTI CTTAAG 1 cut(s) 1010
BsrDI GCAATG 1 cut(s) 1080
BsrFI RCCGGY 1 cut(s) 108
BsrI ACTGG 7 cut(s) 151, 177, 456, 581, 932, 985, 1341
BssAI RCCGGY 1 cut(s) 108
BssECI CCNNGG 1 cut(s) 832
BssMI GATC 7 cut(s) 199, 421, 433, 558, 916, 1173, 1278
BssNI GRCGYC 2 cut(s) 57, 1312
BssSI CACGAG 1 cut(s) 207
BssT1I CCWWGG 1 cut(s) 832
Bst2BI CACGAG 1 cut(s) 207
Bst2UI CCWGG 1 cut(s) 92
Bst4CI ACNGT 3 cut(s) 410, 1059, 1223
Bst6I CTCTTC 3 cut(s) 55, 519, 1344
BstACI GRCGYC 2 cut(s) 57, 1312
BstAFI CTTAAG 1 cut(s) 1010
BstAPI GCANNNNNTGC 2 cut(s) 278, 1364
BstBAI YACGTR 3 cut(s) 478, 709, 1095
BstC8I GCNNGC 9 cut(s) 61, 110, 158, 162, 488, 492, 1083, 1316, 1342
BstDEI CTNAG 1 cut(s) 531
BstF5I GGATG 1 cut(s) 877
BstH2I RGCGCY 1 cut(s) 1315
BstHHI GCGC 2 cut(s) 142, 1314
BstKTI GATC 7 cut(s) 202, 424, 436, 561, 919, 1176, 1281
BstMAI GTCTC 1 cut(s) 306
BstMBI GATC 7 cut(s) 199, 421, 433, 558, 916, 1173, 1278
BstMWI GCNNNNNNNGC 7 cut(s) 77, 278, 343, 536, 1253, 1273, 1364
BstNI CCWGG 1 cut(s) 92
BstNSI RCATGY 2 cut(s) 490, 857
BstSCI CCNGG 1 cut(s) 90
BstSFI CTRYAG 1 cut(s) 406
BstSLI GKGCMC 1 cut(s) 391
BstV1I GCAGC 8 cut(s) 149, 163, 268, 526, 790, 1065, 1215, 1234
BstV2I GAAGAC 1 cut(s) 899
BstX2I RGATCY 1 cut(s) 433
BstXI CCANNNNNNTGG 1 cut(s) 1387
BstYI RGATCY 1 cut(s) 433
BsuI GTATCC 3 cut(s) 26, 545, 1307
BsuRI GGCC 4 cut(s) 108, 150, 389, 732
BtrI CACGTC 2 cut(s) 809, 952
BtsCI GGATG 1 cut(s) 877
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 4 cut(s) 126, 158, 279, 415
Cac8I GCNNGC 9 cut(s) 61, 110, 158, 162, 488, 492, 1083, 1316, 1342
CfoI GCGC 2 cut(s) 142, 1314
Cfr10I RCCGGY 1 cut(s) 108
Cfr13I GGNCC 5 cut(s) 45, 387, 388, 883, 1142
CseI GACGC 1 cut(s) 65
CsiI ACCWGGT 1 cut(s) 90
Csp6I GTAC 5 cut(s) 262, 481, 978, 1096, 1219
CviAII CATG 9 cut(s) 487, 569, 599, 643, 663, 685, 734, 854, 1063
CviQI GTAC 5 cut(s) 262, 481, 978, 1096, 1219
DdeI CTNAG 1 cut(s) 531
DinI GGCGCC 1 cut(s) 1313
DpnI GATC 7 cut(s) 201, 423, 435, 560, 918, 1175, 1280
DpnII GATC 7 cut(s) 199, 421, 433, 558, 916, 1173, 1278
DraI TTTAAA 1 cut(s) 625
EaeI YGGCCR 2 cut(s) 106, 148
Eam1104I CTCTTC 3 cut(s) 55, 519, 1344
EarI CTCTTC 3 cut(s) 55, 519, 1344
EciI GGCGGA 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 832
Eco24I GRGCYC 1 cut(s) 391
Eco47I GGWCC 3 cut(s) 45, 883, 1142
Eco57I CTGAAG 2 cut(s) 36, 590
EcoO109I RGGNCCY 2 cut(s) 45, 387
EcoRII CCWGG 1 cut(s) 90
EcoT14I CCWWGG 1 cut(s) 832
EcoT38I GRGCYC 1 cut(s) 391
EgeI GGCGCC 1 cut(s) 1313
EheI GGCGCC 1 cut(s) 1313
ErhI CCWWGG 1 cut(s) 832
Esp3I CGTCTC 1 cut(s) 306
FaeI CATG 9 cut(s) 490, 572, 602, 646, 666, 688, 737, 857, 1066
FalI AAGNNNNNCTT 2 cut(s) 993, 1025
FaqI GGGAC 1 cut(s) 223
FatI CATG 9 cut(s) 486, 568, 598, 642, 662, 684, 733, 853, 1062
FauI CCCGC 1 cut(s) 1333
FblI GTMKAC 1 cut(s) 819
FokI GGATG 1 cut(s) 884
FriOI GRGCYC 1 cut(s) 391
GlaI GCGC 2 cut(s) 141, 1313
GsaI CCCAGC 1 cut(s) 244
GsuI CTGGAG 1 cut(s) 1340
HaeII RGCGCY 1 cut(s) 1315
HaeIII GGCC 4 cut(s) 108, 150, 389, 732
HapII CCGG 1 cut(s) 109
HgaI GACGC 1 cut(s) 65
HhaI GCGC 2 cut(s) 142, 1314
Hin1I GRCGYC 2 cut(s) 57, 1312
Hin1II CATG 9 cut(s) 490, 572, 602, 646, 666, 688, 737, 857, 1066
Hin6I GCGC 2 cut(s) 140, 1312
HinP1I GCGC 2 cut(s) 140, 1312
HincII GTYRAC 1 cut(s) 972
HindII GTYRAC 1 cut(s) 972
HinfI GANTC 2 cut(s) 750, 774
HpaII CCGG 1 cut(s) 109
HphI GGTGA 5 cut(s) 52, 648, 875, 995, 1199
Hpy166II GTNNAC 6 cut(s) 22, 481, 656, 820, 972, 1190
Hpy188I TCNGA 5 cut(s) 55, 199, 381, 755, 1178
Hpy188III TCNNGA 6 cut(s) 349, 778, 812, 947, 1026, 1145
Hpy8I GTNNAC 6 cut(s) 22, 481, 656, 820, 972, 1190
Hpy99I CGWCG 1 cut(s) 149
HpyAV CCTTC 7 cut(s) 91, 213, 669, 722, 775, 1102, 1312
HpyCH4III ACNGT 3 cut(s) 410, 1059, 1223
HpyCH4IV ACGT 5 cut(s) 477, 708, 808, 951, 1094
HpyF10VI GCNNNNNNNGC 7 cut(s) 77, 278, 343, 536, 1253, 1273, 1364
HpyF3I CTNAG 1 cut(s) 531
HpySE526I ACGT 5 cut(s) 477, 708, 808, 951, 1094
Hsp92I GRCGYC 2 cut(s) 57, 1312
Hsp92II CATG 9 cut(s) 490, 572, 602, 646, 666, 688, 737, 857, 1066
HspAI GCGC 2 cut(s) 140, 1312
KasI GGCGCC 1 cut(s) 1311
KroI GCCGGC 1 cut(s) 108
KroNI GCCGGC 1 cut(s) 110
Kzo9I GATC 7 cut(s) 199, 421, 433, 558, 916, 1173, 1278
LmnI GCTCC 3 cut(s) 217, 742, 1178
Lsp1109I GCAGC 8 cut(s) 149, 163, 268, 526, 790, 1065, 1215, 1234
LweI GCATC 6 cut(s) 72, 790, 862, 952, 1144, 1190
MabI ACCWGGT 1 cut(s) 90
MaeII ACGT 5 cut(s) 477, 708, 808, 951, 1094
MaeIII GTNAC 2 cut(s) 843, 910
MalI GATC 7 cut(s) 201, 423, 435, 560, 918, 1175, 1280
MboI GATC 7 cut(s) 199, 421, 433, 558, 916, 1173, 1278
MfeI CAATTG 1 cut(s) 697
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 4 cut(s) 214, 391, 399, 415
MluCI AATT 9 cut(s) 100, 697, 904, 957, 987, 1004, 1029, 1133, 1148
Mly113I GGCGCC 1 cut(s) 1312
MmeI TCCRAC 2 cut(s) 411, 768
MroNI GCCGGC 1 cut(s) 108
MroXI GAANNNNTTC 1 cut(s) 356
MseI TTAA 6 cut(s) 14, 624, 956, 1011, 1019, 1233
MslI CAYNNNNRTG 1 cut(s) 661
MspA1I CMGCKG 1 cut(s) 244
MspCI CTTAAG 1 cut(s) 1010
MspI CCGG 1 cut(s) 109
MspR9I CCNGG 1 cut(s) 92
MunI CAATTG 1 cut(s) 697
Mva1269I GAATGC 2 cut(s) 337, 832
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 7 cut(s) 77, 278, 343, 536, 1253, 1273, 1364
NaeI GCCGGC 1 cut(s) 110
NarI GGCGCC 1 cut(s) 1312
NdeII GATC 7 cut(s) 199, 421, 433, 558, 916, 1173, 1278
NgoMIV GCCGGC 1 cut(s) 108
NlaIII CATG 9 cut(s) 490, 572, 602, 646, 666, 688, 737, 857, 1066
NlaIV GGNNCC 2 cut(s) 389, 1313
NmuCI GTSAC 1 cut(s) 910
NspI RCATGY 2 cut(s) 490, 857
PaeI GCATGC 1 cut(s) 490
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 153
PctI GAATGC 2 cut(s) 337, 832
PdiI GCCGGC 1 cut(s) 110
PdmI GAANNNNTTC 1 cut(s) 356
PfeI GAWTC 2 cut(s) 750, 774
Pfl23II CGTACG 2 cut(s) 261, 1095
PluTI GGCGCC 1 cut(s) 1315
Ppu21I YACGTR 3 cut(s) 478, 709, 1095
PpuMI RGGWCCY 1 cut(s) 45
Psp5II RGGWCCY 1 cut(s) 45
Psp6I CCWGG 1 cut(s) 90
PspFI CCCAGC 1 cut(s) 240
PspGI CCWGG 1 cut(s) 90
PspLI CGTACG 2 cut(s) 261, 1095
PspN4I GGNNCC 2 cut(s) 389, 1313
PspOMI GGGCCC 1 cut(s) 387
PspPI GGNCC 5 cut(s) 45, 387, 388, 883, 1142
PspPPI RGGWCCY 1 cut(s) 45
PsuI RGATCY 1 cut(s) 433
PvuII CAGCTG 1 cut(s) 244
RsaI GTAC 5 cut(s) 263, 482, 979, 1097, 1220
RsaNI GTAC 5 cut(s) 262, 481, 978, 1096, 1219
RseI CAYNNNNRTG 1 cut(s) 661
SaqAI TTAA 6 cut(s) 14, 624, 956, 1011, 1019, 1233
Sau3AI GATC 7 cut(s) 199, 421, 433, 558, 916, 1173, 1278
Sau96I GGNCC 5 cut(s) 45, 387, 388, 883, 1142
ScrFI CCNGG 1 cut(s) 92
SduI GDGCHC 4 cut(s) 214, 391, 399, 415
SexAI ACCWGGT 1 cut(s) 90
SfaNI GCATC 6 cut(s) 72, 790, 862, 952, 1144, 1190
SfcI CTRYAG 1 cut(s) 406
SfoI GGCGCC 1 cut(s) 1313
SinI GGWCC 3 cut(s) 45, 883, 1142
SmiMI CAYNNNNRTG 1 cut(s) 661
SmlI CTYRAG 2 cut(s) 1010, 1268
SmoI CTYRAG 2 cut(s) 1010, 1268
SphI GCATGC 1 cut(s) 490
Sse9I AATT 9 cut(s) 100, 697, 904, 957, 987, 1004, 1029, 1133, 1148
SsiI CCGC 7 cut(s) 69, 112, 115, 134, 231, 1276, 1340
SspDI GGCGCC 1 cut(s) 1311
StyD4I CCNGG 1 cut(s) 90
StyI CCWWGG 1 cut(s) 832
TaaI ACNGT 3 cut(s) 410, 1059, 1223
TaiI ACGT 5 cut(s) 480, 711, 811, 954, 1097
TaqI TCGA 3 cut(s) 415, 772, 859
TasI AATT 9 cut(s) 100, 697, 904, 957, 987, 1004, 1029, 1133, 1148
TatI WGTACW 1 cut(s) 977
TauI GCSGC 3 cut(s) 115, 137, 233
TfiI GAWTC 2 cut(s) 750, 774
Tru1I TTAA 6 cut(s) 14, 624, 956, 1011, 1019, 1233
Tru9I TTAA 6 cut(s) 14, 624, 956, 1011, 1019, 1233
TscAI CASTG 4 cut(s) 126, 158, 279, 415
TseFI GTSAC 1 cut(s) 910
TseI GCWGC 8 cut(s) 137, 176, 281, 539, 803, 1078, 1228, 1247
Tsp45I GTSAC 1 cut(s) 910
TspDTI ATGAA 9 cut(s) 17, 477, 631, 651, 673, 1051, 1055, 1131, 1224
TspRI CASTG 4 cut(s) 126, 158, 279, 415
Vha464I CTTAAG 1 cut(s) 1010
VpaK11BI GGWCC 3 cut(s) 45, 883, 1142
XapI RAATTY 2 cut(s) 987, 1148
XceI RCATGY 2 cut(s) 490, 857
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XmiI GTMKAC 1 cut(s) 819
XmnI GAANNNNTTC 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.