pycom16g08490

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003753
Physical Location & Seq
Forward (+)
5635414 .. 5637968
2555 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1167 bp
ATGGAATCTCATTTCTCAAAACTACTAGTTGCGACTGCTATGTTAATTTTACTATCAGTGAGCTTGGCAGCCTCATCGTCTCCATGTAAATTTCCGGCCATATTCAACTTCGGTGACTCAAACTCCGACACAGGCGGCTTGTCTGCGCTCTTCGGCCAGGCGCGTTCGCCCCACGGTGAGTCCTACTTCCACAGCCCCGCCGGCCGTTACTGCGATGGCCGTCTCGTCATTGACTTTATAGCGAACAGCCTTGAATTACCGTATCTTAGCGCATATCTTGATTCTGTGGGAAGCAACTTCACTCACGGAGCCAACTTTGCCACGGCGGGCTCAACCATCAGGCCTCAGAACACAACCATTCGCCAGAGCGGGTTTAGTCCCGTCTCCCTGGACGTTCAGTACAATGAGTTCTACGATTTTCAACCAAGATCCCAAACCGCTCGTAGTCGAGGTGGAGTTTTTGAACAGCTAATGCCTAAAGCAGAAGATTTCTCTCGTGCTTTATACACATTTGATATTGGCCAGAATGATTTAACATCCGGTTTGTTCTTGAACATGTCCACTACCCAAGTCAAGGCATATGTTCCTGATGTGCTGAACCAGTTTAGCAGCATTGTTAAGAATGTATACAATCAAGGAGGTAGATACTTTTGGATACACAATACAGGTCCCATTGGTTGTCTTCCCTACGTATTGGACCGCTTACCAATCCCAGCCGCGCAGCTTGACAAGGCCGGATGCGTCAGGCCTTACAATAAACTTGCTCGGTTTTTCAACCGGGGATTGAAACAAGCGGTGGTTCAGTTAAGAAAAGAACTACCATTGGCTGAAATCACTTATGTTGATGTTTATTCAGCCAAGTACTCCCTCATTAGCCAACCACAAAAGCATGGATTCGAGCAACCGATAAGAGCATGCTGTGGTCATGGTGGGAAGTACAATTTCAACAAGCAAATTGGATGTGGAGGAAAGATTAGGGTTCATGGAAAAGATGTGTTAGTGGGAAAGGCATGCCAAGACCCATCACTTTGGGTGAATTGGGATGGTGTGCATTTCACTGAGGCAGCTAACAAGAGAGTCTTCGATCAGATTGTAGATGGTTCACTCTCAGACCCACCAACTCCATTGAAATTTGCTTGCCATGGAAAGCAGCAGCAACCTCATTAG

Protein Analysis

389

Amino Acids

42.79

Weight (kDa)

8.85

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 364 6.1e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 369, 440
AccI GTMKAC 1 cut(s) 627
AccII CGCG 2 cut(s) 163, 719
AciI CCGC 8 cut(s) 135, 198, 326, 369, 438, 700, 717, 794
AclWI GGATC 1 cut(s) 423
AcoI YGGCCR 5 cut(s) 96, 154, 202, 217, 520
AcsI RAATTY 2 cut(s) 89, 1130
AfaI GTAC 3 cut(s) 401, 863, 938
AfiI CCNNNNNNNGG 1 cut(s) 574
AflIII ACRYGT 1 cut(s) 555
AgsI TTSAA 9 cut(s) 106, 254, 422, 464, 553, 775, 787, 946, 1129
AhlI ACTAGT 1 cut(s) 25
AjnI CCWGG 2 cut(s) 156, 387
AluBI AGCT 4 cut(s) 63, 469, 724, 1067
AluI AGCT 4 cut(s) 63, 469, 724, 1067
Alw26I GTCTC 3 cut(s) 84, 227, 388
AlwI GGATC 1 cut(s) 423
AoxI GGCC 8 cut(s) 96, 154, 202, 217, 341, 520, 732, 746
ApeKI GCWGC 6 cut(s) 68, 609, 721, 1064, 1150, 1153
ApoI RAATTY 2 cut(s) 89, 1130
AspLEI GCGC 4 cut(s) 148, 163, 272, 721
AspS9I GGNCC 2 cut(s) 668, 697
AsuC2I CCSGG 1 cut(s) 779
AsuHPI GGTGA 3 cut(s) 125, 188, 1045
AvaII GGWCC 2 cut(s) 668, 697
BalI TGGCCA 1 cut(s) 522
BanII GRGCYC 1 cut(s) 332
BauI CACGAG 1 cut(s) 495
BbsI GAAGAC 2 cut(s) 674, 1072
BbvI GCAGC 5 cut(s) 80, 621, 733, 1076, 1162
BccI CCATC 5 cut(s) 209, 344, 1030, 1037, 1091
BceAI ACGGC 3 cut(s) 189, 204, 339
BcgI CGANNNNNNTGC 2 cut(s) 57, 91
BciT130I CCWGG 2 cut(s) 158, 389
BciVI GTATCC 1 cut(s) 648
BcnI CCSGG 1 cut(s) 779
BcoDI GTCTC 3 cut(s) 84, 227, 388
BcuI ACTAGT 1 cut(s) 25
BfaI CTAG 1 cut(s) 26
BfuI GTATCC 1 cut(s) 648
BglI GCCNNNNNGGC 1 cut(s) 201
BisI GCNGC 8 cut(s) 69, 136, 610, 717, 722, 1065, 1151, 1154
BlsI GCNGC 8 cut(s) 70, 137, 611, 718, 723, 1066, 1152, 1155
BmcAI AGTACT 1 cut(s) 863
Bme1390I CCNGG 3 cut(s) 158, 389, 779
Bme18I GGWCC 2 cut(s) 668, 697
BmgT120I GGNCC 2 cut(s) 668, 697
BmiI GGNNCC 2 cut(s) 310, 670
BmrFI CCNGG 3 cut(s) 158, 389, 779
BmsI GCATC 1 cut(s) 728
BpiI GAAGAC 2 cut(s) 674, 1072
BpuMI CCSGG 1 cut(s) 779
BsaAI YACGTR 1 cut(s) 691
BsaJI CCNNGG 5 cut(s) 172, 321, 387, 778, 1141
BsaWI WCCGGW 1 cut(s) 539
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 1 cut(s) 574
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 1 cut(s) 601
BseBI CCWGG 2 cut(s) 158, 389
BseDI CCNNGG 5 cut(s) 172, 321, 387, 778, 1141
BseGI GGATG 4 cut(s) 536, 743, 965, 1048
BseLI CCNNNNNNNGG 1 cut(s) 574
BseMII CTCAG 3 cut(s) 359, 1050, 1122
BseNI ACTGG 1 cut(s) 601
BseX3I CGGCCG 1 cut(s) 202
BseXI GCAGC 5 cut(s) 80, 621, 733, 1076, 1162
BseYI CCCAGC 1 cut(s) 712
Bsh1236I CGCG 2 cut(s) 163, 719
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 8 cut(s) 98, 156, 204, 219, 343, 522, 734, 748
BsiEI CGRYCG 1 cut(s) 205
BsiSI CCGG 5 cut(s) 95, 201, 540, 735, 778
BslFI GGGAC 2 cut(s) 363, 654
BslI CCNNNNNNNGG 1 cut(s) 574
BsmAI GTCTC 3 cut(s) 84, 227, 388
BsmBI CGTCTC 3 cut(s) 84, 227, 388
BsmFI GGGAC 2 cut(s) 363, 654
BsnI GGCC 8 cut(s) 98, 156, 204, 219, 343, 522, 734, 748
Bsp1286I GDGCHC 1 cut(s) 332
Bsp143I GATC 2 cut(s) 428, 1084
Bsp19I CCATGG 1 cut(s) 1141
BspACI CCGC 8 cut(s) 135, 198, 326, 369, 438, 700, 717, 794
BspANI GGCC 8 cut(s) 98, 156, 204, 219, 343, 522, 734, 748
BspCNI CTCAG 3 cut(s) 358, 1051, 1121
BspFNI CGCG 2 cut(s) 163, 719
BspLI GGNNCC 2 cut(s) 310, 670
BspPI GGATC 1 cut(s) 423
BspQI GCTCTTC 1 cut(s) 155
BsrBI CCGCTC 2 cut(s) 369, 440
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 1 cut(s) 601
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 5 cut(s) 172, 321, 387, 778, 1141
BssMI GATC 2 cut(s) 428, 1084
BssNAI GTATAC 1 cut(s) 628
BssSI CACGAG 1 cut(s) 495
BssT1I CCWWGG 1 cut(s) 1141
Bst1107I GTATAC 1 cut(s) 628
Bst2BI CACGAG 1 cut(s) 495
Bst2UI CCWGG 2 cut(s) 158, 389
Bst4CI ACNGT 2 cut(s) 176, 261
Bst6I CTCTTC 1 cut(s) 155
BstBAI YACGTR 1 cut(s) 691
BstC8I GCNNGC 5 cut(s) 202, 328, 916, 1012, 1138
BstDEI CTNAG 4 cut(s) 266, 345, 1059, 1108
BstDSI CCRYGG 3 cut(s) 172, 321, 1141
BstF5I GGATG 4 cut(s) 536, 743, 965, 1048
BstFNI CGCG 2 cut(s) 163, 719
BstHHI GCGC 4 cut(s) 148, 163, 272, 721
BstKTI GATC 2 cut(s) 431, 1087
BstMAI GTCTC 3 cut(s) 84, 227, 388
BstMBI GATC 2 cut(s) 428, 1084
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 3 cut(s) 201, 210, 317
BstNI CCWGG 2 cut(s) 158, 389
BstNSI RCATGY 3 cut(s) 559, 918, 1014
BstSCI CCNGG 3 cut(s) 156, 387, 777
BstSNI TACGTA 1 cut(s) 691
BstUI CGCG 2 cut(s) 163, 719
BstV1I GCAGC 5 cut(s) 80, 621, 733, 1076, 1162
BstV2I GAAGAC 2 cut(s) 674, 1072
BstX2I RGATCY 1 cut(s) 428
BstXI CCANNNNNNTGG 1 cut(s) 1029
BstYI RGATCY 1 cut(s) 428
BstZ17I GTATAC 1 cut(s) 628
BstZI CGGCCG 1 cut(s) 202
BsuI GTATCC 1 cut(s) 648
BsuRI GGCC 8 cut(s) 98, 156, 204, 219, 343, 522, 734, 748
BtgI CCRYGG 3 cut(s) 172, 321, 1141
BtgZI GCGATG 1 cut(s) 228
BtsCI GGATG 4 cut(s) 536, 743, 965, 1048
BtsIMutI CAGTG 2 cut(s) 63, 1056
Cac8I GCNNGC 5 cut(s) 202, 328, 916, 1012, 1138
CfoI GCGC 4 cut(s) 148, 163, 272, 721
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 2 cut(s) 668, 697
CseI GACGC 1 cut(s) 730
Csp6I GTAC 3 cut(s) 400, 862, 937
CviAII CATG 8 cut(s) 84, 556, 890, 915, 926, 983, 1011, 1142
CviQI GTAC 3 cut(s) 400, 862, 937
DdeI CTNAG 4 cut(s) 266, 345, 1059, 1108
DpnI GATC 2 cut(s) 430, 1086
DpnII GATC 2 cut(s) 428, 1084
EaeI YGGCCR 5 cut(s) 96, 154, 202, 217, 520
EagI CGGCCG 1 cut(s) 202
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
EclXI CGGCCG 1 cut(s) 202
Eco105I TACGTA 1 cut(s) 691
Eco130I CCWWGG 1 cut(s) 1141
Eco147I AGGCCT 2 cut(s) 343, 748
Eco24I GRGCYC 1 cut(s) 332
Eco47I GGWCC 2 cut(s) 668, 697
Eco52I CGGCCG 1 cut(s) 202
EcoO109I RGGNCCY 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 156, 387
EcoT14I CCWWGG 1 cut(s) 1141
EcoT38I GRGCYC 1 cut(s) 332
ErhI CCWWGG 1 cut(s) 1141
Esp3I CGTCTC 3 cut(s) 84, 227, 388
FaeI CATG 8 cut(s) 87, 559, 893, 918, 929, 986, 1014, 1145
FalI AAGNNNNNCTT 2 cut(s) 1064, 1096
FaqI GGGAC 2 cut(s) 363, 654
FatI CATG 8 cut(s) 83, 555, 889, 914, 925, 982, 1010, 1141
FauI CCCGC 3 cut(s) 205, 319, 362
FauNDI CATATG 1 cut(s) 580
FblI GTMKAC 1 cut(s) 627
Fnu4HI GCNGC 8 cut(s) 69, 136, 610, 717, 722, 1065, 1151, 1154
FokI GGATG 4 cut(s) 523, 750, 972, 1055
FriOI GRGCYC 1 cut(s) 332
Fsp4HI GCNGC 8 cut(s) 69, 136, 610, 717, 722, 1065, 1151, 1154
FspBI CTAG 1 cut(s) 26
GlaI GCGC 4 cut(s) 147, 162, 271, 720
GluI GCNGC 8 cut(s) 69, 136, 610, 717, 722, 1065, 1151, 1154
GsaI CCCAGC 1 cut(s) 716
HaeIII GGCC 8 cut(s) 98, 156, 204, 219, 343, 522, 734, 748
HapII CCGG 5 cut(s) 95, 201, 540, 735, 778
HgaI GACGC 1 cut(s) 730
HhaI GCGC 4 cut(s) 148, 163, 272, 721
Hin1II CATG 8 cut(s) 87, 559, 893, 918, 929, 986, 1014, 1145
Hin6I GCGC 4 cut(s) 146, 161, 270, 719
HinP1I GCGC 4 cut(s) 146, 161, 270, 719
HinfI GANTC 6 cut(s) 5, 116, 179, 281, 894, 1077
HpaII CCGG 5 cut(s) 95, 201, 540, 735, 778
HphI GGTGA 3 cut(s) 125, 188, 1045
Hpy166II GTNNAC 3 cut(s) 561, 628, 1103
Hpy188I TCNGA 4 cut(s) 127, 348, 1089, 1111
Hpy188III TCNNGA 3 cut(s) 278, 550, 587
Hpy8I GTNNAC 3 cut(s) 561, 628, 1103
HpyCH4III ACNGT 2 cut(s) 176, 261
HpyCH4IV ACGT 2 cut(s) 393, 690
HpyCH4V TGCA 1 cut(s) 1051
HpyF10VI GCNNNNNNNGC 3 cut(s) 201, 210, 317
HpyF3I CTNAG 4 cut(s) 266, 345, 1059, 1108
HpySE526I ACGT 2 cut(s) 393, 690
Hsp92II CATG 8 cut(s) 87, 559, 893, 918, 929, 986, 1014, 1145
HspAI GCGC 4 cut(s) 146, 161, 270, 719
KroI GCCGGC 1 cut(s) 200
KroNI GCCGGC 1 cut(s) 202
Kzo9I GATC 2 cut(s) 428, 1084
LguI GCTCTTC 1 cut(s) 155
LmnI GCTCC 1 cut(s) 308
Lsp1109I GCAGC 5 cut(s) 80, 621, 733, 1076, 1162
LweI GCATC 1 cut(s) 728
MaeI CTAG 1 cut(s) 26
MaeII ACGT 2 cut(s) 393, 690
MaeIII GTNAC 2 cut(s) 113, 206
MalI GATC 2 cut(s) 430, 1086
MbiI CCGCTC 2 cut(s) 369, 440
MboI GATC 2 cut(s) 428, 1084
MboII GAAGA 4 cut(s) 142, 497, 674, 1072
MflI RGATCY 1 cut(s) 428
MhlI GDGCHC 1 cut(s) 332
MlsI TGGCCA 1 cut(s) 522
MluCI AATT 7 cut(s) 45, 89, 254, 940, 954, 1036, 1130
MluNI TGGCCA 1 cut(s) 522
MlyI GAGTC 3 cut(s) 110, 188, 1086
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 7 cut(s) 82, 354, 443, 632, 878, 959, 1054
Mox20I TGGCCA 1 cut(s) 522
MroNI GCCGGC 1 cut(s) 200
MscI TGGCCA 1 cut(s) 522
MseI TTAA 4 cut(s) 44, 533, 618, 806
Msp20I TGGCCA 1 cut(s) 522
MspI CCGG 5 cut(s) 95, 201, 540, 735, 778
MspR9I CCNGG 3 cut(s) 158, 389, 779
MvaI CCWGG 2 cut(s) 158, 389
MvnI CGCG 2 cut(s) 163, 719
MwoI GCNNNNNNNGC 3 cut(s) 201, 210, 317
NaeI GCCGGC 1 cut(s) 202
NciI CCSGG 1 cut(s) 779
NcoI CCATGG 1 cut(s) 1141
NdeI CATATG 1 cut(s) 580
NdeII GATC 2 cut(s) 428, 1084
NgoMIV GCCGGC 1 cut(s) 200
NlaIII CATG 8 cut(s) 87, 559, 893, 918, 929, 986, 1014, 1145
NlaIV GGNNCC 2 cut(s) 310, 670
NmuCI GTSAC 1 cut(s) 113
NspI RCATGY 3 cut(s) 559, 918, 1014
PaeI GCATGC 2 cut(s) 918, 1014
PceI AGGCCT 2 cut(s) 343, 748
PciI ACATGT 1 cut(s) 555
PciSI GCTCTTC 1 cut(s) 155
PdiI GCCGGC 1 cut(s) 202
PfeI GAWTC 3 cut(s) 5, 281, 894
PkrI GCNGC 8 cut(s) 70, 137, 611, 718, 723, 1066, 1152, 1155
PleI GAGTC 3 cut(s) 110, 187, 1085
PpsI GAGTC 3 cut(s) 110, 187, 1085
Ppu21I YACGTR 1 cut(s) 691
PpuMI RGGWCCY 1 cut(s) 668
PscI ACATGT 1 cut(s) 555
Psp5II RGGWCCY 1 cut(s) 668
Psp6I CCWGG 2 cut(s) 156, 387
PspFI CCCAGC 1 cut(s) 712
PspGI CCWGG 2 cut(s) 156, 387
PspN4I GGNNCC 2 cut(s) 310, 670
PspPI GGNCC 2 cut(s) 668, 697
PspPPI RGGWCCY 1 cut(s) 668
PsrI GAACNNNNNNTAC 2 cut(s) 392, 424
PsuI RGATCY 1 cut(s) 428
RsaI GTAC 3 cut(s) 401, 863, 938
RsaNI GTAC 3 cut(s) 400, 862, 937
SapI GCTCTTC 1 cut(s) 155
SaqAI TTAA 4 cut(s) 44, 533, 618, 806
SatI GCNGC 8 cut(s) 69, 136, 610, 717, 722, 1065, 1151, 1154
Sau3AI GATC 2 cut(s) 428, 1084
Sau96I GGNCC 2 cut(s) 668, 697
ScaI AGTACT 1 cut(s) 863
SchI GAGTC 3 cut(s) 110, 188, 1086
ScrFI CCNGG 3 cut(s) 158, 389, 779
SduI GDGCHC 1 cut(s) 332
SfaNI GCATC 1 cut(s) 728
SinI GGWCC 2 cut(s) 668, 697
SnaBI TACGTA 1 cut(s) 691
SpeI ACTAGT 1 cut(s) 25
SphI GCATGC 2 cut(s) 918, 1014
Sse9I AATT 7 cut(s) 45, 89, 254, 940, 954, 1036, 1130
SseBI AGGCCT 2 cut(s) 343, 748
SsiI CCGC 8 cut(s) 135, 198, 326, 369, 438, 700, 717, 794
SspMI CTAG 1 cut(s) 26
StuI AGGCCT 2 cut(s) 343, 748
StyD4I CCNGG 3 cut(s) 156, 387, 777
StyI CCWWGG 1 cut(s) 1141
TaaI ACNGT 2 cut(s) 176, 261
TaiI ACGT 2 cut(s) 396, 693
TaqI TCGA 3 cut(s) 448, 897, 1083
TasI AATT 7 cut(s) 45, 89, 254, 940, 954, 1036, 1130
TatI WGTACW 3 cut(s) 399, 861, 936
TauI GCSGC 2 cut(s) 138, 719
TfiI GAWTC 3 cut(s) 5, 281, 894
Tru1I TTAA 4 cut(s) 44, 533, 618, 806
Tru9I TTAA 4 cut(s) 44, 533, 618, 806
TscAI CASTG 2 cut(s) 63, 1063
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 6 cut(s) 68, 609, 721, 1064, 1150, 1153
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 1 cut(s) 971
TspGWI ACGGA 1 cut(s) 321
TspRI CASTG 2 cut(s) 63, 1063
VpaK11BI GGWCC 2 cut(s) 668, 697
XapI RAATTY 2 cut(s) 89, 1130
XceI RCATGY 3 cut(s) 559, 918, 1014
XmiI GTMKAC 1 cut(s) 627
XspI CTAG 1 cut(s) 26
ZrmI AGTACT 1 cut(s) 863
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.