pycom16g13110

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003111
Physical Location & Seq
Reverse (-)
9339558 .. 9340769
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1212 bp
ATGGAATTGGCTGGCGGGTTTGCAGCCAAGGATCATAACAAGGTGAAGCACCTTCATGGTGGACGGATGGAATTGGAATCCGAGACTTACTATGTTATCAATCTTCCAGATTCCAAGGTATTGCGGATTATTTCGCGCTCCGTTTTTCTGGGTTTGGTTATTCTCACACTGCCCTGCATTGGGACAATTCTGAGGGGATTAAATGTTTCCCATTTGGATTCGAATATCGAACCCAAAAATTTCAATTTCGAGCAGTTGGGTCTGCTTTTTCGTGATTTGGCCGGCGAAGGCCTCCGCAGAAAGGGTGATGCATCTCTCATTGTGACTCCTGGCAATGCCGGTGCGATTCAAAAGCTTCACCCTTTTGATTTCAATGGATTTGATTTCGTTGTAGGTTCAGATTTGGTGCGAAAGAGCTCGTTCTTGGACGAGTCTATGGATTTCATTTTCGCATTCAACTTGGCCGATGCTAAATTTGCGGATCGCATTCTGAAGATTGGCGGCATAGTTGCCGTTCCAATAAGCAACGATCCTTCAAATGCTTTTAGGCCAAAACCAAATTACAAGATCGTGTATCTTCGCCGATACGCTTCCACTTTCGTGGCAATGAGGAAGATCAGTCCGGCTTATGACTTGCCCGTGACGTCCCGGAAGCTTTTCCAATTAGAAACTGAGGAAAAGAAGACGGTGTTAGAGGGTCTAGAAGACGTGCTGCTGGAACCGCCGAGGCACATTTTGGCCAAGTCAAATGAGCACTTAAACAAGATCAAGTTCCTCCCCGACTTGTTGGGTGATTCCCTCGACATTTACAACCGCCGAGTGTTTGTCAATGTAGACCTAAACGAGGAAAACCACGGCATGGTAGAATGGTTCGATCAAAACTATCCCAAAAAGAACCAAGGATTCGAGGTCTACAACCTCGAAGTAGAGCCTGAAGAGGGTACAACTGCCCCTAAAAACGATGTTTCAGATTGGTTGAAAGACAATGTGAGGGAAGAGGACTATGTCGTGATGAAAGCGGACGCGGAAGTGGTGGTGGAGATGATGAGGAAGAGGACAATCCGTCTGGTGGATGAGTTGTTCTTGGAGTGCAACAATGGATGGTGGGAGAGTGGGAGGAGTGGTGGGAGCAAGAGATCTTATTGGGAATGCATTGCTTTGTATGGGAGGCTGAGGGATCAGGGAGTTGCTGTGCACCAGTGGTTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

404

Amino Acids

45.95

Weight (kDa)

5.82

Isoelectric Point (pI)

41.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7870 PF25276 231 - 402 1.8e-78 Domain of unknown function (DUF7870)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 647
AccB7I CCANNNNNTGG 1 cut(s) 859
AccI GTMKAC 2 cut(s) 834, 912
AccII CGCG 2 cut(s) 136, 1025
AciI CCGC 9 cut(s) 15, 124, 295, 479, 501, 722, 814, 1019, 1025
AclWI GGATC 4 cut(s) 39, 489, 524, 1185
AcoI YGGCCR 3 cut(s) 279, 462, 738
AcsI RAATTY 2 cut(s) 238, 473
AcuI CTGAAG 2 cut(s) 512, 954
AcyI GRCGYC 1 cut(s) 644
AfaI GTAC 1 cut(s) 943
AfiI CCNNNNNNNGG 7 cut(s) 179, 180, 301, 844, 859, 938, 1069
AgsI TTSAA 6 cut(s) 244, 350, 373, 457, 537, 979
AhdI GACNNNNNGTC 1 cut(s) 1062
AjiI CACGTC 1 cut(s) 709
AjnI CCWGG 1 cut(s) 328
AleI CACNNNNGTG 1 cut(s) 599
AloI GAACNNNNNNTCC 2 cut(s) 1064, 1096
AluBI AGCT 3 cut(s) 355, 417, 655
AluI AGCT 3 cut(s) 355, 417, 655
Alw21I GWGCWC 3 cut(s) 419, 756, 1197
Alw26I GTCTC 1 cut(s) 77
Alw44I GTGCAC 1 cut(s) 1193
AlwI GGATC 4 cut(s) 39, 489, 524, 1185
AoxI GGCC 5 cut(s) 279, 289, 462, 548, 738
ApaLI GTGCAC 1 cut(s) 1193
ApeKI GCWGC 2 cut(s) 23, 712
ApoI RAATTY 2 cut(s) 238, 473
Asp700I GAANNNNTTC 1 cut(s) 656
AspLEI GCGC 1 cut(s) 138
AsuC2I CCSGG 1 cut(s) 649
AsuHPI GGTGA 4 cut(s) 55, 317, 350, 803
AsuII TTCGAA 1 cut(s) 221
BaeGI GKGCMC 1 cut(s) 1197
BalI TGGCCA 1 cut(s) 740
BanII GRGCYC 1 cut(s) 419
BbsI GAAGAC 2 cut(s) 689, 711
Bbv12I GWGCWC 3 cut(s) 419, 756, 1197
BbvCI CCTCAGC 1 cut(s) 1172
BbvI GCAGC 2 cut(s) 35, 699
BccI CCATC 2 cut(s) 61, 1095
BceAI ACGGC 2 cut(s) 497, 871
BciT130I CCWGG 1 cut(s) 330
BcnI CCSGG 1 cut(s) 649
BcoDI GTCTC 1 cut(s) 77
BfaI CTAG 1 cut(s) 701
BglII AGATCT 1 cut(s) 1136
BisI GCNGC 3 cut(s) 24, 502, 713
BlsI GCNGC 3 cut(s) 25, 503, 714
Bme1390I CCNGG 2 cut(s) 330, 649
BmeRI GACNNNNNGTC 1 cut(s) 1062
BmgBI CACGTC 1 cut(s) 709
BmiI GGNNCC 1 cut(s) 720
BmrFI CCNGG 2 cut(s) 330, 649
BmsI GCATC 3 cut(s) 298, 320, 457
BpiI GAAGAC 2 cut(s) 689, 711
Bpu10I CCTNAGC 1 cut(s) 1172
Bpu14I TTCGAA 1 cut(s) 221
BpuMI CCSGG 1 cut(s) 649
BsaHI GRCGYC 1 cut(s) 644
BsaJI CCNNGG 5 cut(s) 27, 114, 725, 853, 898
BsaXI ACNNNNNCTCC 4 cut(s) 1108, 1138, 1176, 1206
Bsc4I CCNNNNNNNGG 7 cut(s) 179, 180, 301, 844, 859, 938, 1069
Bse118I RCCGGY 2 cut(s) 281, 338
Bse1I ACTGG 1 cut(s) 1198
Bse3DI GCAATG 3 cut(s) 340, 612, 1152
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 5 cut(s) 27, 114, 725, 853, 898
BseGI GGATG 3 cut(s) 72, 1078, 1106
BseLI CCNNNNNNNGG 7 cut(s) 179, 180, 301, 844, 859, 938, 1069
BseMI GCAATG 3 cut(s) 340, 612, 1152
BseMII CTCAG 3 cut(s) 182, 663, 1163
BseNI ACTGG 1 cut(s) 1198
BseRI GAGGAG 1 cut(s) 1132
BseSI GKGCMC 1 cut(s) 1197
BseXI GCAGC 2 cut(s) 35, 699
Bsh1236I CGCG 2 cut(s) 136, 1025
BshFI GGCC 5 cut(s) 281, 291, 464, 550, 740
BsiHKAI GWGCWC 3 cut(s) 419, 756, 1197
BsiSI CCGG 4 cut(s) 282, 339, 623, 649
BslFI GGGAC 2 cut(s) 196, 631
BslI CCNNNNNNNGG 7 cut(s) 179, 180, 301, 844, 859, 938, 1069
BsmAI GTCTC 1 cut(s) 77
BsmFI GGGAC 2 cut(s) 196, 631
BsmI GAATGC 3 cut(s) 452, 486, 1154
BsnI GGCC 5 cut(s) 281, 291, 464, 550, 740
Bsp119I TTCGAA 1 cut(s) 221
Bsp1286I GDGCHC 3 cut(s) 419, 756, 1197
Bsp143I GATC 9 cut(s) 31, 481, 529, 567, 615, 765, 874, 1136, 1177
BspACI CCGC 9 cut(s) 15, 124, 295, 479, 501, 722, 814, 1019, 1025
BspANI GGCC 5 cut(s) 281, 291, 464, 550, 740
BspCNI CTCAG 3 cut(s) 183, 664, 1164
BspFNI CGCG 2 cut(s) 136, 1025
BspLI GGNNCC 1 cut(s) 720
BspPI GGATC 4 cut(s) 39, 489, 524, 1185
BspT104I TTCGAA 1 cut(s) 221
BsrDI GCAATG 3 cut(s) 340, 612, 1152
BsrFI RCCGGY 2 cut(s) 281, 338
BsrI ACTGG 1 cut(s) 1198
BssAI RCCGGY 2 cut(s) 281, 338
BssECI CCNNGG 5 cut(s) 27, 114, 725, 853, 898
BssMI GATC 9 cut(s) 31, 481, 529, 567, 615, 765, 874, 1136, 1177
BssNI GRCGYC 1 cut(s) 644
BssT1I CCWWGG 3 cut(s) 27, 114, 898
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 1 cut(s) 688
Bst6I CTCTTC 3 cut(s) 930, 990, 1046
BstACI GRCGYC 1 cut(s) 644
BstBI TTCGAA 1 cut(s) 221
BstC8I GCNNGC 2 cut(s) 13, 283
BstDEI CTNAG 3 cut(s) 191, 672, 1172
BstDSI CCRYGG 1 cut(s) 853
BstENI CCTNNNNNAGG 1 cut(s) 842
BstF5I GGATG 3 cut(s) 72, 1078, 1106
BstFNI CGCG 2 cut(s) 136, 1025
BstHHI GCGC 1 cut(s) 138
BstKTI GATC 9 cut(s) 34, 484, 532, 570, 618, 768, 877, 1139, 1180
BstMAI GTCTC 1 cut(s) 77
BstMBI GATC 9 cut(s) 31, 481, 529, 567, 615, 765, 874, 1136, 1177
BstMWI GCNNNNNNNGC 2 cut(s) 476, 721
BstNI CCWGG 1 cut(s) 330
BstSCI CCNGG 2 cut(s) 328, 647
BstSLI GKGCMC 1 cut(s) 1197
BstUI CGCG 2 cut(s) 136, 1025
BstV1I GCAGC 2 cut(s) 35, 699
BstV2I GAAGAC 2 cut(s) 689, 711
BstX2I RGATCY 1 cut(s) 1136
BstXI CCANNNNNNTGG 1 cut(s) 601
BstYI RGATCY 1 cut(s) 1136
BsuRI GGCC 5 cut(s) 281, 291, 464, 550, 740
BtgI CCRYGG 1 cut(s) 853
BtrI CACGTC 1 cut(s) 709
BtsCI GGATG 3 cut(s) 72, 1078, 1106
BtsI GCAGTG 1 cut(s) 167
BtsIMutI CAGTG 2 cut(s) 167, 1205
Cac8I GCNNGC 2 cut(s) 13, 283
CfoI GCGC 1 cut(s) 138
Cfr10I RCCGGY 2 cut(s) 281, 338
CseI GACGC 1 cut(s) 1031
Csp6I GTAC 1 cut(s) 942
CviAII CATG 2 cut(s) 56, 859
CviQI GTAC 1 cut(s) 942
DdeI CTNAG 3 cut(s) 191, 672, 1172
DpnI GATC 9 cut(s) 33, 483, 531, 569, 617, 767, 876, 1138, 1179
DpnII GATC 9 cut(s) 31, 481, 529, 567, 615, 765, 874, 1136, 1177
DriI GACNNNNNGTC 1 cut(s) 1062
EaeI YGGCCR 3 cut(s) 279, 462, 738
Eam1104I CTCTTC 3 cut(s) 930, 990, 1046
Eam1105I GACNNNNNGTC 1 cut(s) 1062
EarI CTCTTC 3 cut(s) 930, 990, 1046
Ecl136II GAGCTC 1 cut(s) 417
Eco130I CCWWGG 3 cut(s) 27, 114, 898
Eco147I AGGCCT 1 cut(s) 291
Eco24I GRGCYC 1 cut(s) 419
Eco53kI GAGCTC 1 cut(s) 417
Eco57I CTGAAG 2 cut(s) 512, 954
EcoICRI GAGCTC 1 cut(s) 417
EcoNI CCTNNNNNAGG 1 cut(s) 842
EcoRII CCWGG 1 cut(s) 328
EcoT14I CCWWGG 3 cut(s) 27, 114, 898
EcoT22I ATGCAT 2 cut(s) 313, 1154
EcoT38I GRGCYC 1 cut(s) 419
ErhI CCWWGG 3 cut(s) 27, 114, 898
FaeI CATG 2 cut(s) 59, 862
FaiI YATR 9 cut(s) 36, 57, 93, 437, 506, 630, 860, 1005, 1164
FaqI GGGAC 2 cut(s) 196, 631
FatI CATG 2 cut(s) 55, 858
FauI CCCGC 1 cut(s) 8
FblI GTMKAC 2 cut(s) 834, 912
Fnu4HI GCNGC 3 cut(s) 24, 502, 713
FokI GGATG 3 cut(s) 79, 1085, 1113
FriOI GRGCYC 1 cut(s) 419
Fsp4HI GCNGC 3 cut(s) 24, 502, 713
FspBI CTAG 1 cut(s) 701
GlaI GCGC 1 cut(s) 137
GluI GCNGC 3 cut(s) 24, 502, 713
HaeIII GGCC 5 cut(s) 281, 291, 464, 550, 740
HapII CCGG 4 cut(s) 282, 339, 623, 649
HgaI GACGC 1 cut(s) 1031
HhaI GCGC 1 cut(s) 138
Hin1I GRCGYC 1 cut(s) 644
Hin1II CATG 2 cut(s) 59, 862
Hin6I GCGC 1 cut(s) 136
HinP1I GCGC 1 cut(s) 136
HindIII AAGCTT 2 cut(s) 353, 653
HinfI GANTC 8 cut(s) 77, 110, 218, 325, 346, 431, 794, 903
HpaII CCGG 4 cut(s) 282, 339, 623, 649
HphI GGTGA 4 cut(s) 55, 317, 350, 803
Hpy166II GTNNAC 4 cut(s) 62, 835, 913, 1195
Hpy188I TCNGA 5 cut(s) 82, 192, 400, 492, 970
Hpy188III TCNNGA 4 cut(s) 107, 272, 701, 1009
Hpy8I GTNNAC 4 cut(s) 62, 835, 913, 1195
HpyAV CCTTC 3 cut(s) 62, 281, 543
HpyCH4III ACNGT 1 cut(s) 688
HpyCH4IV ACGT 2 cut(s) 644, 708
HpyCH4V TGCA 6 cut(s) 23, 177, 311, 1092, 1152, 1195
HpyF10VI GCNNNNNNNGC 2 cut(s) 476, 721
HpyF3I CTNAG 3 cut(s) 191, 672, 1172
HpySE526I ACGT 2 cut(s) 644, 708
Hsp92I GRCGYC 1 cut(s) 644
Hsp92II CATG 2 cut(s) 59, 862
HspAI GCGC 1 cut(s) 136
KroI GCCGGC 1 cut(s) 281
KroNI GCCGGC 1 cut(s) 283
Kzo9I GATC 9 cut(s) 31, 481, 529, 567, 615, 765, 874, 1136, 1177
LmnI GCTCC 2 cut(s) 143, 1128
Lsp1109I GCAGC 2 cut(s) 35, 699
LweI GCATC 3 cut(s) 298, 320, 457
MaeI CTAG 1 cut(s) 701
MaeII ACGT 2 cut(s) 644, 708
MaeIII GTNAC 2 cut(s) 322, 640
MalI GATC 9 cut(s) 33, 483, 531, 569, 617, 767, 876, 1138, 1179
MboI GATC 9 cut(s) 31, 481, 529, 567, 615, 765, 874, 1136, 1177
MboII GAAGA 9 cut(s) 95, 505, 569, 625, 694, 716, 947, 1007, 1063
MflI RGATCY 1 cut(s) 1136
MhlI GDGCHC 3 cut(s) 419, 756, 1197
MlsI TGGCCA 1 cut(s) 740
MluCI AATT 8 cut(s) 5, 71, 186, 238, 244, 473, 559, 662
MluNI TGGCCA 1 cut(s) 740
MlyI GAGTC 2 cut(s) 319, 440
Mox20I TGGCCA 1 cut(s) 740
Mph1103I ATGCAT 2 cut(s) 313, 1154
MroNI GCCGGC 1 cut(s) 281
MroXI GAANNNNTTC 1 cut(s) 656
MscI TGGCCA 1 cut(s) 740
MseI TTAA 2 cut(s) 200, 758
MslI CAYNNNNRTG 2 cut(s) 54, 599
Msp20I TGGCCA 1 cut(s) 740
MspI CCGG 4 cut(s) 282, 339, 623, 649
MspR9I CCNGG 2 cut(s) 330, 649
Mva1269I GAATGC 3 cut(s) 452, 486, 1154
MvaI CCWGG 1 cut(s) 330
MvnI CGCG 2 cut(s) 136, 1025
MwoI GCNNNNNNNGC 2 cut(s) 476, 721
NaeI GCCGGC 1 cut(s) 283
NciI CCSGG 1 cut(s) 649
NdeII GATC 9 cut(s) 31, 481, 529, 567, 615, 765, 874, 1136, 1177
NgoMIV GCCGGC 1 cut(s) 281
NlaIII CATG 2 cut(s) 59, 862
NlaIV GGNNCC 1 cut(s) 720
NmeAIII GCCGAG 2 cut(s) 750, 842
NmuCI GTSAC 2 cut(s) 322, 640
NsiI ATGCAT 2 cut(s) 313, 1154
NspV TTCGAA 1 cut(s) 221
OliI CACNNNNGTG 1 cut(s) 599
PceI AGGCCT 1 cut(s) 291
PctI GAATGC 3 cut(s) 452, 486, 1154
PdiI GCCGGC 1 cut(s) 283
PdmI GAANNNNTTC 1 cut(s) 656
PfeI GAWTC 6 cut(s) 77, 110, 218, 346, 794, 903
PflFI GACNNNGTC 1 cut(s) 1004
PflMI CCANNNNNTGG 1 cut(s) 859
PfoI TCCNGGA 1 cut(s) 647
PkrI GCNGC 3 cut(s) 25, 503, 714
PleI GAGTC 2 cut(s) 319, 439
PpsI GAGTC 2 cut(s) 319, 439
Psp124BI GAGCTC 1 cut(s) 419
Psp6I CCWGG 1 cut(s) 328
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 1 cut(s) 720
PsuI RGATCY 1 cut(s) 1136
PsyI GACNNNGTC 1 cut(s) 1004
RsaI GTAC 1 cut(s) 943
RsaNI GTAC 1 cut(s) 942
RseI CAYNNNNRTG 2 cut(s) 54, 599
SacI GAGCTC 1 cut(s) 419
SaqAI TTAA 2 cut(s) 200, 758
SatI GCNGC 3 cut(s) 24, 502, 713
Sau3AI GATC 9 cut(s) 31, 481, 529, 567, 615, 765, 874, 1136, 1177
SchI GAGTC 2 cut(s) 319, 440
ScrFI CCNGG 2 cut(s) 330, 649
SduI GDGCHC 3 cut(s) 419, 756, 1197
SfaNI GCATC 3 cut(s) 298, 320, 457
SfuI TTCGAA 1 cut(s) 221
SmiMI CAYNNNNRTG 2 cut(s) 54, 599
Sse9I AATT 8 cut(s) 5, 71, 186, 238, 244, 473, 559, 662
SseBI AGGCCT 1 cut(s) 291
SsiI CCGC 9 cut(s) 15, 124, 295, 479, 501, 722, 814, 1019, 1025
SspMI CTAG 1 cut(s) 701
SstI GAGCTC 1 cut(s) 419
StuI AGGCCT 1 cut(s) 291
StyD4I CCNGG 2 cut(s) 328, 647
StyI CCWWGG 3 cut(s) 27, 114, 898
TaaI ACNGT 1 cut(s) 688
TaiI ACGT 2 cut(s) 647, 711
TaqI TCGA 7 cut(s) 221, 228, 249, 801, 873, 906, 921
TasI AATT 8 cut(s) 5, 71, 186, 238, 244, 473, 559, 662
TauI GCSGC 1 cut(s) 504
TfiI GAWTC 6 cut(s) 77, 110, 218, 346, 794, 903
Tru1I TTAA 2 cut(s) 200, 758
Tru9I TTAA 2 cut(s) 200, 758
TscAI CASTG 2 cut(s) 174, 1205
TseFI GTSAC 2 cut(s) 322, 640
TseI GCWGC 2 cut(s) 23, 712
Tsp45I GTSAC 2 cut(s) 322, 640
TspDTI ATGAA 3 cut(s) 44, 433, 1028
TspGWI ACGGA 3 cut(s) 79, 130, 1052
TspRI CASTG 2 cut(s) 174, 1205
Tth111I GACNNNGTC 1 cut(s) 1004
Van91I CCANNNNNTGG 1 cut(s) 859
VneI GTGCAC 1 cut(s) 1193
XagI CCTNNNNNAGG 1 cut(s) 842
XapI RAATTY 2 cut(s) 238, 473
XbaI TCTAGA 1 cut(s) 700
XmiI GTMKAC 2 cut(s) 834, 912
XmnI GAANNNNTTC 1 cut(s) 656
XspI CTAG 1 cut(s) 701
ZraI GACGTC 1 cut(s) 645
Zsp2I ATGCAT 2 cut(s) 313, 1154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.