pycom16g13280
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0005553
Physical Location & Seq
Forward (+)
9442190 .. 9443297
1108 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 450 bp
ATGTTTCTGGTGGATTGGTTCTATGGAGTTCTTGCGTCGCTTGGGCTATGGCAGAAGGAGGCCAAGATCTTGTTCTTAGGGCTCGATAATGCCGGAAAGACCACCTTGCTTCACATGCTCAAAGACGAGAGATTAGTTCAGCACCAACCGACGCAGCATCCGACGTCGGAGGAGCTGAGTATTGGGAAGATTAAGTTCAAGGCCTTTGATTTGGGAGGGCATCAGGTCGCTCGCAGAGTTTGGAAGGACTATTACGCCAAGGTGGATGCTGTGGTCTACCTGGTTGATGCTTACGACAAGGAGAGATTTGCAGAGTCAAAGAAGGAGCTTGATGCTCTACTCTCTGACGAGTCACTGGCAACTGTCCCGTTTCTTATTTTGGGAAACAAGATCGATCATATGTACCATATGCTGCATCAGAGGATGAGCTACGTTACCATTTGGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

150

Amino Acids

17.26

Weight (kDa)

6.65

Isoelectric Point (pI)

29.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 8 - 142 6.2e-53 ADP-ribosylation factor family
SRPRB PF09439 19 - 133 1.4e-07 Signal recognition particle receptor beta subunit
Roc PF08477 22 - 132 8.4e-09 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 22 - 130 4.5e-08 50S ribosome-binding GTPase
Ras PF00071 22 - 133 6.2e-07 Ras family
G-alpha PF00503 48 - 139 3.5e-10 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 167
AccI GTMKAC 1 cut(s) 276
AcyI GRCGYC 1 cut(s) 164
AfaI GTAC 1 cut(s) 404
AgsI TTSAA 1 cut(s) 199
AjnI CCWGG 1 cut(s) 279
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AluBI AGCT 3 cut(s) 175, 328, 429
AluI AGCT 3 cut(s) 175, 328, 429
AoxI GGCC 3 cut(s) 60, 201, 444
ApeKI GCWGC 2 cut(s) 154, 412
AspS9I GGNCC 1 cut(s) 444
BanII GRGCYC 1 cut(s) 84
BarI GAAGNNNNNNTAC 2 cut(s) 236, 268
BbvI GCAGC 2 cut(s) 166, 399
BciT130I CCWGG 1 cut(s) 281
BglII AGATCT 1 cut(s) 66
BisI GCNGC 2 cut(s) 155, 413
BlsI GCNGC 2 cut(s) 156, 414
Bme1390I CCNGG 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 444
BmrFI CCNGG 1 cut(s) 281
BmsI GCATC 6 cut(s) 166, 229, 256, 277, 322, 424
Bsa29I ATCGAT 1 cut(s) 393
BsaHI GRCGYC 1 cut(s) 164
BsaJI CCNNGG 1 cut(s) 258
Bse1I ACTGG 1 cut(s) 360
BseBI CCWGG 1 cut(s) 281
BseCI ATCGAT 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 258
BseGI GGATG 3 cut(s) 157, 271, 429
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 1 cut(s) 360
BseRI GAGGAG 1 cut(s) 185
BseXI GCAGC 2 cut(s) 166, 399
BshFI GGCC 3 cut(s) 62, 203, 446
BshVI ATCGAT 1 cut(s) 393
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 3 cut(s) 62, 203, 446
Bsp1286I GDGCHC 1 cut(s) 84
Bsp143I GATC 3 cut(s) 66, 390, 394
BspANI GGCC 3 cut(s) 62, 203, 446
BspCNI CTCAG 1 cut(s) 168
BspDI ATCGAT 1 cut(s) 393
BsrI ACTGG 1 cut(s) 360
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 3 cut(s) 66, 390, 394
BssNI GRCGYC 1 cut(s) 164
BssT1I CCWWGG 1 cut(s) 258
Bst2UI CCWGG 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 364
BstACI GRCGYC 1 cut(s) 164
BstC8I GCNNGC 1 cut(s) 232
BstDEI CTNAG 2 cut(s) 76, 176
BstF5I GGATG 3 cut(s) 157, 271, 429
BstKTI GATC 3 cut(s) 69, 393, 397
BstMBI GATC 3 cut(s) 66, 390, 394
BstMWI GCNNNNNNNGC 1 cut(s) 115
BstNI CCWGG 1 cut(s) 281
BstNSI RCATGY 1 cut(s) 118
BstSCI CCNGG 1 cut(s) 279
BstV1I GCAGC 2 cut(s) 166, 399
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
Bsu15I ATCGAT 1 cut(s) 393
BsuRI GGCC 3 cut(s) 62, 203, 446
BsuTUI ATCGAT 1 cut(s) 393
BtsCI GGATG 3 cut(s) 157, 271, 429
BtsIMutI CAGTG 1 cut(s) 353
Cac8I GCNNGC 1 cut(s) 232
Cfr13I GGNCC 1 cut(s) 444
ClaI ATCGAT 1 cut(s) 393
CseI GACGC 2 cut(s) 24, 160
CsiI ACCWGGT 1 cut(s) 279
Csp6I GTAC 1 cut(s) 403
CviAII CATG 1 cut(s) 115
CviJI RGCY 8 cut(s) 46, 62, 82, 175, 203, 328, 429, 446
CviKI_1 RGCY 8 cut(s) 46, 62, 82, 175, 203, 328, 429, 446
CviQI GTAC 1 cut(s) 403
DdeI CTNAG 2 cut(s) 76, 176
DpnI GATC 3 cut(s) 68, 392, 396
DpnII GATC 3 cut(s) 66, 390, 394
Eco130I CCWWGG 1 cut(s) 258
Eco147I AGGCCT 1 cut(s) 203
Eco24I GRGCYC 1 cut(s) 84
EcoRII CCWGG 1 cut(s) 279
EcoT14I CCWWGG 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 84
ErhI CCWWGG 1 cut(s) 258
FaeI CATG 1 cut(s) 118
FaiI YATR 7 cut(s) 24, 49, 116, 399, 401, 408, 410
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FaqI GGGAC 1 cut(s) 350
FatI CATG 1 cut(s) 114
FauNDI CATATG 2 cut(s) 399, 408
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 2 cut(s) 155, 413
FokI GGATG 3 cut(s) 144, 278, 436
FriOI GRGCYC 1 cut(s) 84
Fsp4HI GCNGC 2 cut(s) 155, 413
GluI GCNGC 2 cut(s) 155, 413
HaeIII GGCC 3 cut(s) 62, 203, 446
HapII CCGG 1 cut(s) 93
HgaI GACGC 2 cut(s) 24, 160
Hin1I GRCGYC 1 cut(s) 164
Hin1II CATG 1 cut(s) 118
HinfI GANTC 2 cut(s) 314, 350
HpaII CCGG 1 cut(s) 93
Hpy166II GTNNAC 1 cut(s) 277
Hpy188I TCNGA 4 cut(s) 162, 169, 346, 420
Hpy8I GTNNAC 1 cut(s) 277
Hpy99I CGWCG 4 cut(s) 40, 154, 166, 169
HpyAV CCTTC 3 cut(s) 49, 238, 316
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 2 cut(s) 164, 432
HpyCH4V TGCA 2 cut(s) 311, 415
HpyF10VI GCNNNNNNNGC 1 cut(s) 115
HpyF3I CTNAG 2 cut(s) 76, 176
HpySE526I ACGT 2 cut(s) 164, 432
Hsp92I GRCGYC 1 cut(s) 164
Hsp92II CATG 1 cut(s) 118
Kzo9I GATC 3 cut(s) 66, 390, 394
LmnI GCTCC 2 cut(s) 172, 325
LpnPI CCDG 5 cut(s) 106, 209, 266, 293, 341
Lsp1109I GCAGC 2 cut(s) 166, 399
LweI GCATC 6 cut(s) 166, 229, 256, 277, 322, 424
MabI ACCWGGT 1 cut(s) 279
MaeII ACGT 2 cut(s) 164, 432
MaeIII GTNAC 2 cut(s) 351, 433
MalI GATC 3 cut(s) 68, 392, 396
MboI GATC 3 cut(s) 66, 390, 394
MboII GAAGA 1 cut(s) 199
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 1 cut(s) 84
MlyI GAGTC 2 cut(s) 323, 359
MmeI TCCRAC 2 cut(s) 147, 185
MnlI CCTC 4 cut(s) 52, 163, 209, 414
MseI TTAA 1 cut(s) 192
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 281
MvaI CCWGG 1 cut(s) 281
MwoI GCNNNNNNNGC 1 cut(s) 115
NdeI CATATG 2 cut(s) 399, 408
NdeII GATC 3 cut(s) 66, 390, 394
NlaIII CATG 1 cut(s) 118
NmuCI GTSAC 1 cut(s) 351
NspI RCATGY 1 cut(s) 118
PceI AGGCCT 1 cut(s) 203
PcsI WCGNNNNNNNCGW 1 cut(s) 158
PkrI GCNGC 2 cut(s) 156, 414
PleI GAGTC 2 cut(s) 322, 358
PpsI GAGTC 2 cut(s) 322, 358
Psp6I CCWGG 1 cut(s) 279
PspGI CCWGG 1 cut(s) 279
PspPI GGNCC 1 cut(s) 444
PsuI RGATCY 1 cut(s) 66
RsaI GTAC 1 cut(s) 404
RsaNI GTAC 1 cut(s) 403
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 2 cut(s) 155, 413
Sau3AI GATC 3 cut(s) 66, 390, 394
Sau96I GGNCC 1 cut(s) 444
SchI GAGTC 2 cut(s) 323, 359
ScrFI CCNGG 1 cut(s) 281
SduI GDGCHC 1 cut(s) 84
SetI ASST 9 cut(s) 107, 167, 177, 228, 264, 282, 330, 431, 435
SexAI ACCWGGT 1 cut(s) 279
SfaNI GCATC 6 cut(s) 166, 229, 256, 277, 322, 424
SseBI AGGCCT 1 cut(s) 203
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 279
StyI CCWWGG 1 cut(s) 258
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 2 cut(s) 167, 435
TaqI TCGA 2 cut(s) 84, 393
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TscAI CASTG 1 cut(s) 360
TseFI GTSAC 1 cut(s) 351
TseI GCWGC 2 cut(s) 154, 412
Tsp45I GTSAC 1 cut(s) 351
TspRI CASTG 1 cut(s) 360
XceI RCATGY 1 cut(s) 118
XmiI GTMKAC 1 cut(s) 276
ZraI GACGTC 1 cut(s) 165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.