RLG00000006460
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0005553
Physical Location & Seq
Forward (+)
7441096 .. 7441559
464 bp
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UTR
Exon/CDS
Intron
RLM00000006460

Sequence Viewer

Length: 315 bp
ATGGTGCGATCTGATGAGGCTCTGATCCAATCCATTCCGCTTCGATCCGCGTACGTCATCATGTTTCTCTTCGACTGGTTCTACGGTGTGCTCACTTCCATCGGTCTGTGGCAGAAGGAGGCCAAGATCTTGATCCTAGGGCTCGATAATGCCGGCAAGACCAACTTGCTTCACATGCTCAAAGATGAGCTTCACAAACTGCTGGAGCTTGAAGCTGATGAGGTTTTGGATAGGTTGGATGATCTGGGAGACCAATTTGATATGAAAGCTTTGGTCTTTCACATTTCTGGAGTCTTGGGTTTTCTCAGTGTTTAA

Protein Analysis

105

Amino Acids

11.82

Weight (kDa)

4.69

Isoelectric Point (pI)

34.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 28 - 63 3.2e-09 ADP-ribosylation factor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 50
AciI CCGC 2 cut(s) 38, 48
AclWI GGATC 3 cut(s) 19, 39, 127
AfaI GTAC 1 cut(s) 53
AgsI TTSAA 1 cut(s) 212
AluBI AGCT 4 cut(s) 190, 208, 215, 269
AluI AGCT 4 cut(s) 190, 208, 215, 269
Alw21I GWGCWC 1 cut(s) 93
Alw26I GTCTC 1 cut(s) 243
AlwI GGATC 3 cut(s) 19, 39, 127
AoxI GGCC 1 cut(s) 120
AspA2I CCTAGG 1 cut(s) 136
AvrII CCTAGG 1 cut(s) 136
BanII GRGCYC 1 cut(s) 144
Bbv12I GWGCWC 1 cut(s) 93
BccI CCATC 1 cut(s) 107
BcoDI GTCTC 1 cut(s) 243
BfaI CTAG 1 cut(s) 137
BglII AGATCT 1 cut(s) 126
BlnI CCTAGG 1 cut(s) 136
BpmI CTGGAG 2 cut(s) 224, 309
BsaBI GATNNNNATC 1 cut(s) 131
BsaI GGTCTC 1 cut(s) 243
BsaJI CCNNGG 1 cut(s) 136
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 1 cut(s) 80
Bse8I GATNNNNATC 1 cut(s) 131
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 1 cut(s) 244
BseJI GATNNNNATC 1 cut(s) 131
BseNI ACTGG 1 cut(s) 80
Bsh1236I CGCG 1 cut(s) 50
BshFI GGCC 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 93
BsiSI CCGG 1 cut(s) 153
BsiWI CGTACG 1 cut(s) 51
BsmAI GTCTC 1 cut(s) 243
BsnI GGCC 1 cut(s) 122
Bso31I GGTCTC 1 cut(s) 243
Bsp1286I GDGCHC 2 cut(s) 93, 144
Bsp143I GATC 6 cut(s) 8, 24, 44, 126, 132, 241
BspACI CCGC 2 cut(s) 38, 48
BspANI GGCC 1 cut(s) 122
BspFNI CGCG 1 cut(s) 50
BspPI GGATC 3 cut(s) 19, 39, 127
BspTNI GGTCTC 1 cut(s) 243
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 1 cut(s) 80
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 6 cut(s) 8, 24, 44, 126, 132, 241
BssT1I CCWWGG 1 cut(s) 136
Bst4CI ACNGT 1 cut(s) 86
Bst6I CTCTTC 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 154
BstDEI CTNAG 1 cut(s) 305
BstF5I GGATG 1 cut(s) 244
BstFNI CGCG 1 cut(s) 50
BstKTI GATC 6 cut(s) 11, 27, 47, 129, 135, 244
BstMAI GTCTC 1 cut(s) 243
BstMBI GATC 6 cut(s) 8, 24, 44, 126, 132, 241
BstMWI GCNNNNNNNGC 1 cut(s) 175
BstNSI RCATGY 1 cut(s) 178
BstUI CGCG 1 cut(s) 50
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BsuRI GGCC 1 cut(s) 122
BtsCI GGATG 1 cut(s) 244
BtsIMutI CAGTG 1 cut(s) 313
Cac8I GCNNGC 1 cut(s) 154
Cfr10I RCCGGY 1 cut(s) 152
Csp6I GTAC 1 cut(s) 52
CviAII CATG 2 cut(s) 61, 175
CviJI RGCY 7 cut(s) 20, 122, 142, 190, 208, 215, 269
CviKI_1 RGCY 7 cut(s) 20, 122, 142, 190, 208, 215, 269
CviQI GTAC 1 cut(s) 52
DdeI CTNAG 1 cut(s) 305
DpnI GATC 6 cut(s) 10, 26, 46, 128, 134, 243
DpnII GATC 6 cut(s) 8, 24, 44, 126, 132, 241
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 136
Eco24I GRGCYC 1 cut(s) 144
Eco31I GGTCTC 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 136
EcoT38I GRGCYC 1 cut(s) 144
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 2 cut(s) 64, 178
FaiI YATR 3 cut(s) 62, 176, 263
FalI AAGNNNNNCTT 4 cut(s) 149, 181, 174, 206
FatI CATG 2 cut(s) 60, 174
FokI GGATG 1 cut(s) 251
FriOI GRGCYC 1 cut(s) 144
FspBI CTAG 1 cut(s) 137
GsuI CTGGAG 2 cut(s) 224, 309
HaeIII GGCC 1 cut(s) 122
HapII CCGG 1 cut(s) 153
Hin1II CATG 2 cut(s) 64, 178
HindIII AAGCTT 1 cut(s) 267
HinfI GANTC 1 cut(s) 291
HpaII CCGG 1 cut(s) 153
Hpy188I TCNGA 2 cut(s) 13, 24
Hpy188III TCNNGA 2 cut(s) 130, 288
HpyAV CCTTC 1 cut(s) 109
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4IV ACGT 1 cut(s) 54
HpyF10VI GCNNNNNNNGC 1 cut(s) 175
HpyF3I CTNAG 1 cut(s) 305
HpySE526I ACGT 1 cut(s) 54
Hsp92II CATG 2 cut(s) 64, 178
KroI GCCGGC 1 cut(s) 152
KroNI GCCGGC 1 cut(s) 154
Kzo9I GATC 6 cut(s) 8, 24, 44, 126, 132, 241
LmnI GCTCC 1 cut(s) 205
LpnPI CCDG 5 cut(s) 61, 166, 188, 230, 273
MaeI CTAG 1 cut(s) 137
MaeII ACGT 1 cut(s) 54
MalI GATC 6 cut(s) 10, 26, 46, 128, 134, 243
MboI GATC 6 cut(s) 8, 24, 44, 126, 132, 241
MboII GAAGA 1 cut(s) 61
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 2 cut(s) 93, 144
MluCI AATT 1 cut(s) 254
MlyI GAGTC 1 cut(s) 300
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 3 cut(s) 10, 112, 214
MroNI GCCGGC 1 cut(s) 152
MseI TTAA 1 cut(s) 313
MspI CCGG 1 cut(s) 153
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 1 cut(s) 175
NaeI GCCGGC 1 cut(s) 154
NdeII GATC 6 cut(s) 8, 24, 44, 126, 132, 241
NgoMIV GCCGGC 1 cut(s) 152
NlaIII CATG 2 cut(s) 64, 178
NspI RCATGY 1 cut(s) 178
PdiI GCCGGC 1 cut(s) 154
Pfl23II CGTACG 1 cut(s) 51
PleI GAGTC 1 cut(s) 299
PpsI GAGTC 1 cut(s) 299
PspLI CGTACG 1 cut(s) 51
PsuI RGATCY 1 cut(s) 126
RsaI GTAC 1 cut(s) 53
RsaNI GTAC 1 cut(s) 52
SaqAI TTAA 1 cut(s) 313
Sau3AI GATC 6 cut(s) 8, 24, 44, 126, 132, 241
SchI GAGTC 1 cut(s) 300
SduI GDGCHC 2 cut(s) 93, 144
SetI ASST 7 cut(s) 57, 192, 210, 217, 225, 236, 271
Sse9I AATT 1 cut(s) 254
SsiI CCGC 2 cut(s) 38, 48
SspMI CTAG 1 cut(s) 137
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 86
TaiI ACGT 1 cut(s) 57
TaqI TCGA 3 cut(s) 43, 72, 144
TaqII GACCGA 1 cut(s) 92
TasI AATT 1 cut(s) 254
Tru1I TTAA 1 cut(s) 313
Tru9I TTAA 1 cut(s) 313
TscAI CASTG 1 cut(s) 313
TspDTI ATGAA 1 cut(s) 278
TspRI CASTG 1 cut(s) 313
XceI RCATGY 1 cut(s) 178
XmaJI CCTAGG 1 cut(s) 136
XspI CTAG 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.