RLG00000033226
ERF Family

Belongs to the small GTPase superfamily. SAR1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0005553
Physical Location & Seq
Forward (+)
24619697 .. 24620532
836 bp
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UTR
Exon/CDS
Intron
RLM00000033226

Sequence Viewer

Length: 363 bp
ATGTTTCTATTCGACTGGTTCTACGACGTGCTGGCTTCCCTCGGTCTATGGCAGAAGAAGGCCAAGATCTTATTCCTAGCGCTCGATAATGCTGGCAAGACCACCTTGCTTCACATGCTCAAAGATGAGAGGCTGGTGCAGCATCAACCTACGCAATATCCCACATCTGAGGAGTTGAGTATTGGCAAAATCAAGTTCAAGGCCTTTGATTTGGGAGGTCATCAGATTGCTCGCAGGGTCTGGAAGGCAAAGGTAGATGCTGTGGTGTACCCTGTGGATGCCTACGATGGTTTTAAGTGGCTCTCTCAATACATTACTATGAATTGGTTGTTCAAAGTTCTGAGCAGTGCCTTGTGTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 ko05134 map04141 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

121

Amino Acids

13.9

Weight (kDa)

9.21

Isoelectric Point (pI)

27.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 7 - 96 2.3e-29 ADP-ribosylation factor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 269
AfeI AGCGCT 1 cut(s) 81
AgsI TTSAA 2 cut(s) 199, 334
AjiI CACGTC 1 cut(s) 28
AjuI GAANNNNNNNTTGG 2 cut(s) 56, 88
AlwNI CAGNNNCTG 1 cut(s) 240
Aor51HI AGCGCT 1 cut(s) 81
AoxI GGCC 2 cut(s) 60, 201
ApeKI GCWGC 1 cut(s) 139
AspLEI GCGC 1 cut(s) 82
BbvI GCAGC 1 cut(s) 151
BccI CCATC 1 cut(s) 281
BfaI CTAG 1 cut(s) 77
BfoI RGCGCY 1 cut(s) 83
BglII AGATCT 1 cut(s) 66
BisI GCNGC 1 cut(s) 140
BlsI GCNGC 1 cut(s) 141
BmgBI CACGTC 1 cut(s) 28
BmsI GCATC 3 cut(s) 151, 247, 268
BsaJI CCNNGG 1 cut(s) 40
Bse1I ACTGG 1 cut(s) 20
BseDI CCNNGG 1 cut(s) 40
BseGI GGATG 1 cut(s) 283
BseMII CTCAG 2 cut(s) 159, 332
BseNI ACTGG 1 cut(s) 20
BseRI GAGGAG 1 cut(s) 185
BseXI GCAGC 1 cut(s) 151
BsgI GTGCAG 1 cut(s) 158
BshFI GGCC 2 cut(s) 62, 203
BsnI GGCC 2 cut(s) 62, 203
Bsp143I GATC 1 cut(s) 66
BspANI GGCC 2 cut(s) 62, 203
BspCNI CTCAG 2 cut(s) 160, 333
BsrI ACTGG 1 cut(s) 20
BssECI CCNNGG 1 cut(s) 40
BssMI GATC 1 cut(s) 66
BstC8I GCNNGC 3 cut(s) 33, 94, 232
BstDEI CTNAG 2 cut(s) 168, 341
BstF5I GGATG 1 cut(s) 283
BstH2I RGCGCY 1 cut(s) 83
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 1 cut(s) 69
BstMBI GATC 1 cut(s) 66
BstMWI GCNNNNNNNGC 2 cut(s) 115, 139
BstNSI RCATGY 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 151
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuRI GGCC 2 cut(s) 62, 203
BtrI CACGTC 1 cut(s) 28
BtsCI GGATG 1 cut(s) 283
BtsI GCAGTG 1 cut(s) 352
BtsIMutI CAGTG 1 cut(s) 352
Cac8I GCNNGC 3 cut(s) 33, 94, 232
CaiI CAGNNNCTG 1 cut(s) 240
CfoI GCGC 1 cut(s) 82
Csp6I GTAC 1 cut(s) 268
CviAII CATG 1 cut(s) 115
CviJI RGCY 5 cut(s) 35, 62, 133, 203, 301
CviKI_1 RGCY 5 cut(s) 35, 62, 133, 203, 301
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 2 cut(s) 168, 341
DpnI GATC 1 cut(s) 68
DpnII GATC 1 cut(s) 66
Eco147I AGGCCT 1 cut(s) 203
Eco47III AGCGCT 1 cut(s) 81
FaeI CATG 1 cut(s) 118
FaiI YATR 3 cut(s) 49, 116, 320
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FatI CATG 1 cut(s) 114
Fnu4HI GCNGC 1 cut(s) 140
FokI GGATG 1 cut(s) 290
Fsp4HI GCNGC 1 cut(s) 140
FspBI CTAG 1 cut(s) 77
GlaI GCGC 1 cut(s) 81
GluI GCNGC 1 cut(s) 140
HaeII RGCGCY 1 cut(s) 83
HaeIII GGCC 2 cut(s) 62, 203
HhaI GCGC 1 cut(s) 82
Hin1II CATG 1 cut(s) 118
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
Hpy166II GTNNAC 1 cut(s) 268
Hpy188I TCNGA 3 cut(s) 169, 225, 342
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 1 cut(s) 268
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 2 cut(s) 52, 238
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 1 cut(s) 139
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 139
HpyF3I CTNAG 2 cut(s) 168, 341
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 1 cut(s) 118
HspAI GCGC 1 cut(s) 80
Kzo9I GATC 1 cut(s) 66
LpnPI CCDG 6 cut(s) 17, 78, 119, 220, 226, 285
Lsp1109I GCAGC 1 cut(s) 151
LweI GCATC 3 cut(s) 151, 247, 268
MaeI CTAG 1 cut(s) 77
MaeII ACGT 1 cut(s) 27
MalI GATC 1 cut(s) 68
MboI GATC 1 cut(s) 66
MboII GAAGA 1 cut(s) 67
MflI RGATCY 1 cut(s) 66
MluCI AATT 1 cut(s) 322
MnlI CCTC 4 cut(s) 50, 123, 163, 209
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 317
MwoI GCNNNNNNNGC 2 cut(s) 115, 139
NdeII GATC 1 cut(s) 66
NlaIII CATG 1 cut(s) 118
NspI RCATGY 1 cut(s) 118
PceI AGGCCT 1 cut(s) 203
PkrI GCNGC 1 cut(s) 141
PstNI CAGNNNCTG 1 cut(s) 240
PsuI RGATCY 1 cut(s) 66
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
RseI CAYNNNNRTG 1 cut(s) 317
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 1 cut(s) 140
Sau3AI GATC 1 cut(s) 66
SetI ASST 5 cut(s) 30, 107, 151, 220, 255
SfaNI GCATC 3 cut(s) 151, 247, 268
SmiMI CAYNNNNRTG 1 cut(s) 317
Sse9I AATT 1 cut(s) 322
SseBI AGGCCT 1 cut(s) 203
SspMI CTAG 1 cut(s) 77
StuI AGGCCT 1 cut(s) 203
TaiI ACGT 1 cut(s) 30
TaqI TCGA 2 cut(s) 12, 84
TaqII GACCGA 1 cut(s) 32
TasI AATT 1 cut(s) 322
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 1 cut(s) 352
TseI GCWGC 1 cut(s) 139
TspDTI ATGAA 1 cut(s) 335
TspRI CASTG 1 cut(s) 352
XceI RCATGY 1 cut(s) 118
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.