pycom16g14270

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0006630
Physical Location & Seq
Reverse (-)
10302332 .. 10303138
807 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 807 bp
ATGGTTCGGTCTTTTGTTCTTATTTCAGGCATGGTCAACGGCGCCTCTCACGGATCGATCAAGAAGAAGAAGATGAAACCTCGCAACGCCTCCTCGTCCATTCAATCTCTTCCGAACGAGGTTCTGGTCCAAGTACTCGCGAAGGTCGCTTCGCGCTCCTTCGATGATCTTTATTCAGCCATGTTAAGCTGCAAGAATTTCAACGAAAGTGCCCAAGATGATCACATATTCGAGCACGTCGACATTAGCAAGTTCCCGCTGGTTTCCTGGCGCGCAAGTGAAAAACACACGAGGTTTCTGAAGCGGTGCAGGGATCTCGGCAATGCGGAGGCCTTGTACAGGCAAGGGATGCGCGACCTCTTCACAAGCAAGAGGATAGAGCCTGCGGGGGTCGAGTCTCTGAAGAGGGCAGCCTCAAAGGGTCACGTAGAAGCAACATACGTGTACGGAGCAATCTTGGTGTGTTACGGGGGCGATTCAAGGCAGAAAGGACTCGAGCTTCTGTACTCACTCAACCGCCACAAATCGAGAGGGTTAAGCGTGACAGAATGCCGAGAGAGGATTAAGGAATTGATTTGGAACACAATGTGGGTGAATAGGGAAGTAATAGGTAGAATTGTAGAAGCACACGATGAGAACACTGCCATGAAAGCATGCAACGACTGTGGAGATCCACAAGGTCCGTACACAATCGAACAAGGATGGGATTTCGATGACCATGATAAATTCAGTAGTTGCAATTCATGCAGATGGCATCAGGAAGTAAATATATTTTGTGATATATTATGTCGCAGTCGTACAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

269

Amino Acids

30.49

Weight (kDa)

8.76

Isoelectric Point (pI)

48.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 34 - 82 1.3e-07 F-box-like
TPR_27 PF23310 84 - 193 4.1e-36 Plant tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 41
AccI GTMKAC 1 cut(s) 240
AccII CGCG 4 cut(s) 140, 154, 273, 354
AciI CCGC 5 cut(s) 257, 304, 326, 386, 517
AclWI GGATC 3 cut(s) 61, 321, 665
AcsI RAATTY 2 cut(s) 196, 725
AcuI CTGAAG 2 cut(s) 320, 422
AcyI GRCGYC 1 cut(s) 42
AdeI CACNNNGTG 1 cut(s) 588
AfaI GTAC 6 cut(s) 135, 338, 446, 506, 686, 799
AfiI CCNNNNNNNGG 1 cut(s) 339
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 3 cut(s) 104, 202, 480
AjiI CACGTC 1 cut(s) 238
AjnI CCWGG 1 cut(s) 266
AluBI AGCT 2 cut(s) 189, 499
AluI AGCT 2 cut(s) 189, 499
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 3 cut(s) 61, 321, 665
Ama87I CYCGRG 1 cut(s) 494
AoxI GGCC 1 cut(s) 330
ApeKI GCWGC 2 cut(s) 189, 410
ApoI RAATTY 2 cut(s) 196, 725
AspLEI GCGC 5 cut(s) 44, 156, 273, 275, 354
AspS9I GGNCC 2 cut(s) 127, 680
AsuHPI GGTGA 1 cut(s) 604
AvaI CYCGRG 1 cut(s) 494
AvaII GGWCC 2 cut(s) 127, 680
BaeGI GKGCMC 1 cut(s) 214
BanI GGYRCC 1 cut(s) 41
BauI CACGAG 1 cut(s) 289
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 2 cut(s) 176, 422
BccI CCATC 2 cut(s) 696, 744
BceAI ACGGC 1 cut(s) 55
BcgI CGANNNNNNTGC 2 cut(s) 298, 332
BciT130I CCWGG 1 cut(s) 268
BclI TGATCA 1 cut(s) 220
BcoDI GTCTC 1 cut(s) 402
BfoI RGCGCY 1 cut(s) 45
BisI GCNGC 2 cut(s) 190, 411
BlsI GCNGC 2 cut(s) 191, 412
BmcAI AGTACT 1 cut(s) 135
Bme1390I CCNGG 1 cut(s) 268
Bme18I GGWCC 2 cut(s) 127, 680
BmeT110I CYCGRG 1 cut(s) 494
BmgBI CACGTC 1 cut(s) 238
BmgT120I GGNCC 2 cut(s) 127, 680
BmiI GGNNCC 1 cut(s) 43
BmrFI CCNGG 1 cut(s) 268
BmsI GCATC 2 cut(s) 339, 763
Bsa29I ATCGAT 1 cut(s) 56
BsaAI YACGTR 2 cut(s) 427, 442
BsaHI GRCGYC 1 cut(s) 42
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 1 cut(s) 339
Bse3DI GCAATG 1 cut(s) 328
BseBI CCWGG 1 cut(s) 268
BseCI ATCGAT 1 cut(s) 56
BseGI GGATG 2 cut(s) 354, 707
BseLI CCNNNNNNNGG 1 cut(s) 339
BseMI GCAATG 1 cut(s) 328
BsePI GCGCGC 1 cut(s) 271
BseRI GAGGAG 1 cut(s) 82
BseSI GKGCMC 1 cut(s) 214
BseXI GCAGC 2 cut(s) 176, 422
BsgI GTGCAG 1 cut(s) 328
Bsh1236I CGCG 4 cut(s) 140, 154, 273, 354
BshFI GGCC 1 cut(s) 332
BshNI GGYRCC 1 cut(s) 41
BshVI ATCGAT 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 494
BslI CCNNNNNNNGG 1 cut(s) 339
BsmAI GTCTC 1 cut(s) 402
BsmI GAATGC 1 cut(s) 554
BsnI GGCC 1 cut(s) 332
BsoBI CYCGRG 1 cut(s) 494
Bsp1286I GDGCHC 2 cut(s) 214, 237
Bsp1407I TGTACA 1 cut(s) 336
Bsp143I GATC 6 cut(s) 53, 57, 166, 220, 313, 670
Bsp68I TCGCGA 1 cut(s) 140
BspACI CCGC 5 cut(s) 257, 304, 326, 386, 517
BspANI GGCC 1 cut(s) 332
BspDI ATCGAT 1 cut(s) 56
BspFNI CGCG 4 cut(s) 140, 154, 273, 354
BspLI GGNNCC 1 cut(s) 43
BspPI GGATC 3 cut(s) 61, 321, 665
BspT107I GGYRCC 1 cut(s) 41
BsrDI GCAATG 1 cut(s) 328
BsrGI TGTACA 1 cut(s) 336
BssHII GCGCGC 1 cut(s) 271
BssMI GATC 6 cut(s) 53, 57, 166, 220, 313, 670
BssNI GRCGYC 1 cut(s) 42
BssSI CACGAG 1 cut(s) 289
Bst2BI CACGAG 1 cut(s) 289
Bst2UI CCWGG 1 cut(s) 268
Bst4CI ACNGT 1 cut(s) 665
Bst6I CTCTTC 3 cut(s) 114, 365, 398
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 2 cut(s) 349, 744
BstAUI TGTACA 1 cut(s) 336
BstBAI YACGTR 2 cut(s) 427, 442
BstC8I GCNNGC 3 cut(s) 273, 384, 655
BstENI CCTNNNNNAGG 1 cut(s) 337
BstF5I GGATG 2 cut(s) 354, 707
BstFNI CGCG 4 cut(s) 140, 154, 273, 354
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 5 cut(s) 44, 156, 273, 275, 354
BstKTI GATC 6 cut(s) 56, 60, 169, 223, 316, 673
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 6 cut(s) 53, 57, 166, 220, 313, 670
BstMWI GCNNNNNNNGC 4 cut(s) 146, 349, 650, 744
BstNI CCWGG 1 cut(s) 268
BstNSI RCATGY 1 cut(s) 657
BstSCI CCNGG 1 cut(s) 266
BstSLI GKGCMC 1 cut(s) 214
BstUI CGCG 4 cut(s) 140, 154, 273, 354
BstV1I GCAGC 2 cut(s) 176, 422
BstX2I RGATCY 2 cut(s) 313, 670
BstYI RGATCY 2 cut(s) 313, 670
Bsu15I ATCGAT 1 cut(s) 56
BsuRI GGCC 1 cut(s) 332
BsuTUI ATCGAT 1 cut(s) 56
BtrI CACGTC 1 cut(s) 238
BtsCI GGATG 2 cut(s) 354, 707
BtsI GCAGTG 1 cut(s) 639
BtsIMutI CAGTG 1 cut(s) 639
BtuMI TCGCGA 1 cut(s) 140
Cac8I GCNNGC 3 cut(s) 273, 384, 655
CfoI GCGC 5 cut(s) 44, 156, 273, 275, 354
Cfr13I GGNCC 2 cut(s) 127, 680
ClaI ATCGAT 1 cut(s) 56
Csp6I GTAC 6 cut(s) 134, 337, 445, 505, 685, 798
CspCI CAANNNNNGTGG 2 cut(s) 646, 681
CviAII CATG 6 cut(s) 31, 181, 646, 654, 719, 744
CviJI RGCY 6 cut(s) 179, 189, 332, 382, 413, 499
CviKI_1 RGCY 6 cut(s) 179, 189, 332, 382, 413, 499
CviQI GTAC 6 cut(s) 134, 337, 445, 505, 685, 798
DinI GGCGCC 1 cut(s) 43
DpnI GATC 6 cut(s) 55, 59, 168, 222, 315, 672
DpnII GATC 6 cut(s) 53, 57, 166, 220, 313, 670
DraIII CACNNNGTG 1 cut(s) 588
Eam1104I CTCTTC 3 cut(s) 114, 365, 398
EarI CTCTTC 3 cut(s) 114, 365, 398
Eco147I AGGCCT 1 cut(s) 332
Eco47I GGWCC 2 cut(s) 127, 680
Eco57I CTGAAG 2 cut(s) 320, 422
Eco88I CYCGRG 1 cut(s) 494
EcoNI CCTNNNNNAGG 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 266
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
FaeI CATG 6 cut(s) 34, 184, 649, 657, 722, 747
FatI CATG 6 cut(s) 30, 180, 645, 653, 718, 743
FauI CCCGC 2 cut(s) 264, 379
FbaI TGATCA 1 cut(s) 220
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 2 cut(s) 190, 411
FokI GGATG 2 cut(s) 361, 714
Fsp4HI GCNGC 2 cut(s) 190, 411
GlaI GCGC 5 cut(s) 43, 155, 272, 274, 353
GluI GCNGC 2 cut(s) 190, 411
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 1 cut(s) 332
HhaI GCGC 5 cut(s) 44, 156, 273, 275, 354
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 6 cut(s) 34, 184, 649, 657, 722, 747
Hin6I GCGC 5 cut(s) 42, 154, 271, 273, 352
HinP1I GCGC 5 cut(s) 42, 154, 271, 273, 352
HincII GTYRAC 2 cut(s) 37, 241
HindII GTYRAC 2 cut(s) 37, 241
HinfI GANTC 3 cut(s) 395, 476, 492
HphI GGTGA 1 cut(s) 604
Hpy166II GTNNAC 4 cut(s) 37, 241, 445, 687
Hpy188I TCNGA 3 cut(s) 114, 300, 402
Hpy188III TCNNGA 4 cut(s) 61, 139, 528, 758
Hpy8I GTNNAC 4 cut(s) 37, 241, 445, 687
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 2 cut(s) 136, 169
HpyCH4III ACNGT 1 cut(s) 665
HpyCH4IV ACGT 3 cut(s) 237, 426, 441
HpyCH4V TGCA 5 cut(s) 192, 309, 657, 738, 747
HpyF10VI GCNNNNNNNGC 4 cut(s) 146, 349, 650, 744
HpySE526I ACGT 3 cut(s) 237, 426, 441
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 6 cut(s) 34, 184, 649, 657, 722, 747
HspAI GCGC 5 cut(s) 42, 154, 271, 273, 352
KasI GGCGCC 1 cut(s) 41
Ksp22I TGATCA 1 cut(s) 220
Kzo9I GATC 6 cut(s) 53, 57, 166, 220, 313, 670
LmnI GCTCC 2 cut(s) 161, 449
LpnPI CCDG 9 cut(s) 12, 110, 245, 253, 280, 295, 325, 396, 743
Lsp1109I GCAGC 2 cut(s) 176, 422
LweI GCATC 2 cut(s) 339, 763
MaeII ACGT 3 cut(s) 237, 426, 441
MaeIII GTNAC 3 cut(s) 422, 464, 541
MalI GATC 6 cut(s) 55, 59, 168, 222, 315, 672
MboI GATC 6 cut(s) 53, 57, 166, 220, 313, 670
MboII GAAGA 6 cut(s) 76, 79, 82, 101, 352, 415
MflI RGATCY 2 cut(s) 313, 670
MhlI GDGCHC 2 cut(s) 214, 237
MluCI AATT 6 cut(s) 196, 569, 615, 725, 739, 802
Mly113I GGCGCC 1 cut(s) 42
MlyI GAGTC 2 cut(s) 404, 486
MseI TTAA 3 cut(s) 185, 536, 564
MslI CAYNNNNRTG 2 cut(s) 644, 748
MspA1I CMGCKG 1 cut(s) 259
MspR9I CCNGG 1 cut(s) 268
Mva1269I GAATGC 1 cut(s) 554
MvaI CCWGG 1 cut(s) 268
MvnI CGCG 4 cut(s) 140, 154, 273, 354
MwoI GCNNNNNNNGC 4 cut(s) 146, 349, 650, 744
NarI GGCGCC 1 cut(s) 42
NdeII GATC 6 cut(s) 53, 57, 166, 220, 313, 670
NlaIII CATG 6 cut(s) 34, 184, 649, 657, 722, 747
NlaIV GGNNCC 1 cut(s) 43
NmeAIII GCCGAG 2 cut(s) 297, 578
NmuCI GTSAC 2 cut(s) 422, 541
NruI TCGCGA 1 cut(s) 140
NspI RCATGY 1 cut(s) 657
PaeI GCATGC 1 cut(s) 657
PaeR7I CTCGAG 1 cut(s) 494
PauI GCGCGC 1 cut(s) 271
PceI AGGCCT 1 cut(s) 332
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PctI GAATGC 1 cut(s) 554
PfeI GAWTC 1 cut(s) 476
PkrI GCNGC 2 cut(s) 191, 412
PleI GAGTC 2 cut(s) 403, 486
PluTI GGCGCC 1 cut(s) 45
PpsI GAGTC 2 cut(s) 403, 486
Ppu21I YACGTR 2 cut(s) 427, 442
Psp6I CCWGG 1 cut(s) 266
PspGI CCWGG 1 cut(s) 266
PspN4I GGNNCC 1 cut(s) 43
PspPI GGNCC 2 cut(s) 127, 680
PspXI VCTCGAGB 1 cut(s) 494
PsuI RGATCY 2 cut(s) 313, 670
PteI GCGCGC 1 cut(s) 271
RruI TCGCGA 1 cut(s) 140
RsaI GTAC 6 cut(s) 135, 338, 446, 506, 686, 799
RsaNI GTAC 6 cut(s) 134, 337, 445, 505, 685, 798
RseI CAYNNNNRTG 2 cut(s) 644, 748
SalI GTCGAC 1 cut(s) 239
SaqAI TTAA 3 cut(s) 185, 536, 564
SatI GCNGC 2 cut(s) 190, 411
Sau3AI GATC 6 cut(s) 53, 57, 166, 220, 313, 670
Sau96I GGNCC 2 cut(s) 127, 680
ScaI AGTACT 1 cut(s) 135
SchI GAGTC 2 cut(s) 404, 486
ScrFI CCNGG 1 cut(s) 268
SduI GDGCHC 2 cut(s) 214, 237
SfaNI GCATC 2 cut(s) 339, 763
SfoI GGCGCC 1 cut(s) 43
Sfr274I CTCGAG 1 cut(s) 494
SinI GGWCC 2 cut(s) 127, 680
SlaI CTCGAG 1 cut(s) 494
SmiMI CAYNNNNRTG 2 cut(s) 644, 748
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
SphI GCATGC 1 cut(s) 657
Sse9I AATT 6 cut(s) 196, 569, 615, 725, 739, 802
SseBI AGGCCT 1 cut(s) 332
SsiI CCGC 5 cut(s) 257, 304, 326, 386, 517
SspDI GGCGCC 1 cut(s) 41
StuI AGGCCT 1 cut(s) 332
StyD4I CCNGG 1 cut(s) 266
TaaI ACNGT 1 cut(s) 665
TaiI ACGT 3 cut(s) 240, 429, 444
TaqI TCGA 9 cut(s) 56, 162, 231, 240, 393, 495, 527, 693, 711
TasI AATT 6 cut(s) 196, 569, 615, 725, 739, 802
TatI WGTACW 3 cut(s) 133, 336, 504
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 3 cut(s) 185, 536, 564
Tru9I TTAA 3 cut(s) 185, 536, 564
TscAI CASTG 1 cut(s) 646
TseFI GTSAC 2 cut(s) 422, 541
TseI GCWGC 2 cut(s) 189, 410
Tsp45I GTSAC 2 cut(s) 422, 541
TspDTI ATGAA 3 cut(s) 89, 662, 732
TspGWI ACGGA 3 cut(s) 66, 462, 672
TspRI CASTG 1 cut(s) 646
VpaK11BI GGWCC 2 cut(s) 127, 680
XagI CCTNNNNNAGG 1 cut(s) 337
XapI RAATTY 2 cut(s) 196, 725
XceI RCATGY 1 cut(s) 657
XhoI CTCGAG 1 cut(s) 494
XmiI GTMKAC 1 cut(s) 240
ZrmI AGTACT 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.