pycom16g14570

Lipase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0002948
Physical Location & Seq
Forward (+)
10663627 .. 10667244
3618 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1020 bp
ATGTTTGTTTTTTCCGCTGGAAGAGAACTGAAGATTAAGCACAAGGATCATGGCCCCATATACAATCACACGCTTGCCACTATACTAGTGCGGTATGCTTCTACGGTATATGTGTCTGATTTGTCGGAGCTGTTTACTTGGACATGCTCGTGCTGTAATGGTTTGATTAAGGGATTTGAAATGATAGAGATTATTGTTGACGTCCAGCATTGCTTACAGGCTTTTGTTGGAGTGGCACAAGACCCGAATGCTATTATAATTGCATTTAGGGGGACTCAGGAACACAGCATACAGAATTGGATTGAAGACTTATTTTGGAAACAACTTGATCTAGATTATCCTGGCATGCCTGATTCAATGGTGCACCATGGGTTTTATAATGCTTACCACAACACAACCATACGCCCTGGAATTCTAAATGCTGTGGAAAGAGCCAAGGAATATTATGGAGATATTGGCATCATAGTTACAGGACATTCAATGGGAGGGGCAATGGCTTCATTTTGTGCTCTTGATCTAAGGGTCAATCAAAAAGAGAAAGATGTTCAGGTTATGACATTTGGACAACCTCGTATCGGTAATGCAGCATTTGCATCATACTATAGCACACTTGTACCAAACACTATACGAGTAACAAATTCACATGACATGGTGCCTCATTTGCCTCCATACTATACTTATTTTCCTCAGAAGACATACCACCACTTTCCACGAGAGGTGTGGCTTTATAACATTGGAGTTGAAAGTCTTGTTTACGAAGTTGAGAAAATCTGTGATGATTCAGGCGAGGACCCAACGTGCAGCAGGTCAGTGAGTGGGAACAGCATTTCGGACCATTTAGTTTATTATGGCATTGAATTAATGGCCAAGACATGGAGACGGTGCAGAATTGTGATGGGTACTAGTGTAGAAGGGTACAGCAGAACAGATCTAGGAGGAAATTTTGTATTGTCCAGAGATCCTGCAACTTCTGTTCTCAAGTTGAATGCATCGCCAGATACTGGGGCAAAACCTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

340

Amino Acids

38.14

Weight (kDa)

6.04

Isoelectric Point (pI)

29.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 86 - 224 4.7e-37 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 257, 378, 729
AatII GACGTC 1 cut(s) 204
Acc36I ACCTGC 1 cut(s) 795
AccB1I GGYRCC 1 cut(s) 652
AccB7I CCANNNNNTGG 2 cut(s) 873, 1001
AciI CCGC 2 cut(s) 15, 91
AclWI GGATC 2 cut(s) 54, 953
AcoI YGGCCR 1 cut(s) 864
AcsI RAATTY 3 cut(s) 411, 637, 940
AcuI CTGAAG 1 cut(s) 50
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 3 cut(s) 615, 901, 917
AfiI CCNNNNNNNGG 3 cut(s) 575, 873, 1001
AgsI TTSAA 7 cut(s) 179, 305, 357, 480, 743, 857, 985
AhlI ACTAGT 2 cut(s) 85, 902
AjnI CCWGG 2 cut(s) 340, 406
AluBI AGCT 1 cut(s) 130
AluI AGCT 1 cut(s) 130
Alw21I GWGCWC 2 cut(s) 366, 511
Alw26I GTCTC 1 cut(s) 871
Alw44I GTGCAC 1 cut(s) 362
AlwI GGATC 2 cut(s) 54, 953
AlwNI CAGNNNCTG 1 cut(s) 1001
AoxI GGCC 2 cut(s) 52, 864
ApaLI GTGCAC 1 cut(s) 362
ApeKI GCWGC 2 cut(s) 584, 801
ApoI RAATTY 3 cut(s) 411, 637, 940
AseI ATTAAT 1 cut(s) 860
AspS9I GGNCC 3 cut(s) 53, 790, 832
AvaII GGWCC 2 cut(s) 790, 832
BaeGI GKGCMC 1 cut(s) 366
BaeI ACNNNNGTAYC 4 cut(s) 597, 630, 891, 924
BalI TGGCCA 1 cut(s) 866
BanI GGYRCC 1 cut(s) 652
BauI CACGAG 2 cut(s) 148, 711
BbsI GAAGAC 2 cut(s) 312, 698
Bbv12I GWGCWC 2 cut(s) 366, 511
BbvI GCAGC 2 cut(s) 596, 813
BccI CCATC 1 cut(s) 889
BciT130I CCWGG 2 cut(s) 342, 408
BcoDI GTCTC 1 cut(s) 871
BcuI ACTAGT 2 cut(s) 85, 902
BfaI CTAG 4 cut(s) 86, 332, 903, 932
BfmI CTRYAG 2 cut(s) 601, 1016
BfuAI ACCTGC 1 cut(s) 795
BglII AGATCT 1 cut(s) 928
BisI GCNGC 2 cut(s) 585, 802
BlsI GCNGC 2 cut(s) 586, 803
Bme1390I CCNGG 2 cut(s) 342, 408
Bme18I GGWCC 2 cut(s) 790, 832
BmgT120I GGNCC 3 cut(s) 53, 790, 832
BmiI GGNNCC 3 cut(s) 55, 654, 792
BmrFI CCNGG 2 cut(s) 342, 408
BmrI ACTGGG 1 cut(s) 1011
BmsI GCATC 3 cut(s) 468, 602, 998
BmuI ACTGGG 1 cut(s) 1011
BpiI GAAGAC 2 cut(s) 312, 698
BpuEI CTTGAG 1 cut(s) 962
BsaHI GRCGYC 1 cut(s) 201
BsaJI CCNNGG 3 cut(s) 367, 406, 435
Bsc4I CCNNNNNNNGG 3 cut(s) 575, 873, 1001
Bse1I ACTGG 1 cut(s) 1006
Bse3DI GCAATG 2 cut(s) 208, 498
BseBI CCWGG 2 cut(s) 342, 408
BseDI CCNNGG 3 cut(s) 367, 406, 435
BseLI CCNNNNNNNGG 3 cut(s) 575, 873, 1001
BseMI GCAATG 2 cut(s) 208, 498
BseMII CTCAG 2 cut(s) 290, 701
BseNI ACTGG 1 cut(s) 1006
BseSI GKGCMC 1 cut(s) 366
BseXI GCAGC 2 cut(s) 596, 813
BsgI GTGCAG 2 cut(s) 820, 904
BshFI GGCC 2 cut(s) 54, 866
BshNI GGYRCC 1 cut(s) 652
BsiHKAI GWGCWC 2 cut(s) 366, 511
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 3 cut(s) 575, 873, 1001
BsmAI GTCTC 1 cut(s) 871
BsmBI CGTCTC 1 cut(s) 871
BsmFI GGGAC 1 cut(s) 286
BsmI GAATGC 2 cut(s) 253, 991
BsnI GGCC 2 cut(s) 54, 866
Bsp1286I GDGCHC 2 cut(s) 366, 511
Bsp143I GATC 5 cut(s) 46, 328, 514, 928, 958
Bsp19I CCATGG 1 cut(s) 367
BspACI CCGC 2 cut(s) 15, 91
BspANI GGCC 2 cut(s) 54, 866
BspCNI CTCAG 2 cut(s) 289, 700
BspLI GGNNCC 3 cut(s) 55, 654, 792
BspMI ACCTGC 1 cut(s) 795
BspPI GGATC 2 cut(s) 54, 953
BspT107I GGYRCC 1 cut(s) 652
BsrDI GCAATG 2 cut(s) 208, 498
BsrI ACTGG 1 cut(s) 1006
BssECI CCNNGG 3 cut(s) 367, 406, 435
BssMI GATC 5 cut(s) 46, 328, 514, 928, 958
BssNI GRCGYC 1 cut(s) 201
BssSI CACGAG 2 cut(s) 148, 711
BssT1I CCWWGG 2 cut(s) 367, 435
Bst2BI CACGAG 2 cut(s) 148, 711
Bst2UI CCWGG 2 cut(s) 342, 408
Bst4CI ACNGT 2 cut(s) 106, 882
Bst6I CTCTTC 1 cut(s) 16
BstACI GRCGYC 1 cut(s) 201
BstAPI GCANNNNNTGC 1 cut(s) 590
BstC8I GCNNGC 2 cut(s) 75, 347
BstDEI CTNAG 3 cut(s) 276, 518, 687
BstDSI CCRYGG 1 cut(s) 367
BstKTI GATC 5 cut(s) 49, 331, 517, 931, 961
BstMAI GTCTC 1 cut(s) 871
BstMBI GATC 5 cut(s) 46, 328, 514, 928, 958
BstMWI GCNNNNNNNGC 2 cut(s) 590, 661
BstNI CCWGG 2 cut(s) 342, 408
BstNSI RCATGY 2 cut(s) 147, 349
BstSCI CCNGG 2 cut(s) 340, 406
BstSFI CTRYAG 2 cut(s) 601, 1016
BstSLI GKGCMC 1 cut(s) 366
BstV1I GCAGC 2 cut(s) 596, 813
BstV2I GAAGAC 2 cut(s) 312, 698
BstX2I RGATCY 2 cut(s) 928, 958
BstYI RGATCY 2 cut(s) 928, 958
BsuRI GGCC 2 cut(s) 54, 866
BtgI CCRYGG 1 cut(s) 367
BtgZI GCGATG 1 cut(s) 975
BtsIMutI CAGTG 1 cut(s) 816
BveI ACCTGC 1 cut(s) 795
Cac8I GCNNGC 2 cut(s) 75, 347
CaiI CAGNNNCTG 1 cut(s) 1001
Cfr13I GGNCC 3 cut(s) 53, 790, 832
Csp6I GTAC 3 cut(s) 614, 900, 916
CviAII CATG 7 cut(s) 50, 144, 346, 368, 644, 649, 873
CviJI RGCY 7 cut(s) 54, 130, 221, 434, 497, 724, 866
CviKI_1 RGCY 7 cut(s) 54, 130, 221, 434, 497, 724, 866
CviQI GTAC 3 cut(s) 614, 900, 916
DdeI CTNAG 3 cut(s) 276, 518, 687
DpnI GATC 5 cut(s) 48, 330, 516, 930, 960
DpnII GATC 5 cut(s) 46, 328, 514, 928, 958
EaeI YGGCCR 1 cut(s) 864
Eam1104I CTCTTC 1 cut(s) 16
EarI CTCTTC 1 cut(s) 16
Eco130I CCWWGG 2 cut(s) 367, 435
Eco47I GGWCC 2 cut(s) 790, 832
Eco57I CTGAAG 1 cut(s) 50
EcoO109I RGGNCCY 1 cut(s) 790
EcoRI GAATTC 1 cut(s) 411
EcoRII CCWGG 2 cut(s) 340, 406
EcoT14I CCWWGG 2 cut(s) 367, 435
EcoT22I ATGCAT 1 cut(s) 991
ErhI CCWWGG 2 cut(s) 367, 435
Esp3I CGTCTC 1 cut(s) 871
FaeI CATG 7 cut(s) 53, 147, 349, 371, 647, 652, 876
FaqI GGGAC 1 cut(s) 286
FatI CATG 7 cut(s) 49, 143, 345, 367, 643, 648, 872
Fnu4HI GCNGC 2 cut(s) 585, 802
Fsp4HI GCNGC 2 cut(s) 585, 802
FspBI CTAG 4 cut(s) 86, 332, 903, 932
GluI GCNGC 2 cut(s) 585, 802
HaeIII GGCC 2 cut(s) 54, 866
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 7 cut(s) 53, 147, 349, 371, 647, 652, 876
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 3 cut(s) 274, 353, 779
Hpy166II GTNNAC 4 cut(s) 135, 199, 364, 754
Hpy188I TCNGA 4 cut(s) 118, 127, 690, 832
Hpy188III TCNNGA 4 cut(s) 278, 332, 512, 954
Hpy8I GTNNAC 4 cut(s) 135, 199, 364, 754
HpyAV CCTTC 1 cut(s) 905
HpyCH4III ACNGT 2 cut(s) 106, 882
HpyCH4IV ACGT 2 cut(s) 201, 797
HpyCH4V TGCA 8 cut(s) 263, 364, 584, 593, 801, 885, 965, 989
HpyF10VI GCNNNNNNNGC 2 cut(s) 590, 661
HpyF3I CTNAG 3 cut(s) 276, 518, 687
HpySE526I ACGT 2 cut(s) 201, 797
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 7 cut(s) 53, 147, 349, 371, 647, 652, 876
Kzo9I GATC 5 cut(s) 46, 328, 514, 928, 958
LmnI GCTCC 1 cut(s) 127
Lsp1109I GCAGC 2 cut(s) 596, 813
LweI GCATC 3 cut(s) 468, 602, 998
MaeI CTAG 4 cut(s) 86, 332, 903, 932
MaeII ACGT 2 cut(s) 201, 797
MaeIII GTNAC 2 cut(s) 466, 631
MalI GATC 5 cut(s) 48, 330, 516, 930, 960
MboI GATC 5 cut(s) 46, 328, 514, 928, 958
MboII GAAGA 4 cut(s) 33, 43, 317, 703
MflI RGATCY 2 cut(s) 928, 958
MhlI GDGCHC 2 cut(s) 366, 511
MlsI TGGCCA 1 cut(s) 866
MluCI AATT 7 cut(s) 258, 295, 411, 637, 857, 888, 940
MluNI TGGCCA 1 cut(s) 866
MlyI GAGTC 1 cut(s) 268
MmeI TCCRAC 2 cut(s) 105, 208
MnlI CCTC 8 cut(s) 479, 579, 666, 675, 696, 709, 781, 929
Mox20I TGGCCA 1 cut(s) 866
Mph1103I ATGCAT 1 cut(s) 991
MscI TGGCCA 1 cut(s) 866
MseI TTAA 3 cut(s) 36, 168, 860
MslI CAYNNNNRTG 1 cut(s) 148
Msp20I TGGCCA 1 cut(s) 866
MspA1I CMGCKG 1 cut(s) 17
MspR9I CCNGG 2 cut(s) 342, 408
Mva1269I GAATGC 2 cut(s) 253, 991
MvaI CCWGG 2 cut(s) 342, 408
MwoI GCNNNNNNNGC 2 cut(s) 590, 661
NcoI CCATGG 1 cut(s) 367
NdeII GATC 5 cut(s) 46, 328, 514, 928, 958
NlaIII CATG 7 cut(s) 53, 147, 349, 371, 647, 652, 876
NlaIV GGNNCC 3 cut(s) 55, 654, 792
NsiI ATGCAT 1 cut(s) 991
NspI RCATGY 2 cut(s) 147, 349
PaeI GCATGC 1 cut(s) 349
PctI GAATGC 2 cut(s) 253, 991
PfeI GAWTC 2 cut(s) 353, 779
PflMI CCANNNNNTGG 2 cut(s) 873, 1001
PkrI GCNGC 2 cut(s) 586, 803
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PpuMI RGGWCCY 1 cut(s) 790
PshBI ATTAAT 1 cut(s) 860
PsiI TTATAA 3 cut(s) 257, 378, 729
Psp5II RGGWCCY 1 cut(s) 790
Psp6I CCWGG 2 cut(s) 340, 406
PspGI CCWGG 2 cut(s) 340, 406
PspN4I GGNNCC 3 cut(s) 55, 654, 792
PspPI GGNCC 3 cut(s) 53, 790, 832
PspPPI RGGWCCY 1 cut(s) 790
PsrI GAACNNNNNNTAC 2 cut(s) 273, 305
PstNI CAGNNNCTG 1 cut(s) 1001
PsuI RGATCY 2 cut(s) 928, 958
RsaI GTAC 3 cut(s) 615, 901, 917
RsaNI GTAC 3 cut(s) 614, 900, 916
RseI CAYNNNNRTG 1 cut(s) 148
SaqAI TTAA 3 cut(s) 36, 168, 860
SatI GCNGC 2 cut(s) 585, 802
Sau3AI GATC 5 cut(s) 46, 328, 514, 928, 958
Sau96I GGNCC 3 cut(s) 53, 790, 832
SchI GAGTC 1 cut(s) 268
ScrFI CCNGG 2 cut(s) 342, 408
SduI GDGCHC 2 cut(s) 366, 511
SetI ASST 8 cut(s) 132, 204, 552, 571, 720, 800, 809, 1015
SfaNI GCATC 3 cut(s) 468, 602, 998
SfcI CTRYAG 2 cut(s) 601, 1016
SinI GGWCC 2 cut(s) 790, 832
SmiMI CAYNNNNRTG 1 cut(s) 148
SmlI CTYRAG 1 cut(s) 977
SmoI CTYRAG 1 cut(s) 977
SpeI ACTAGT 2 cut(s) 85, 902
SphI GCATGC 1 cut(s) 349
Sse9I AATT 7 cut(s) 258, 295, 411, 637, 857, 888, 940
SsiI CCGC 2 cut(s) 15, 91
SspI AATATT 1 cut(s) 443
SspMI CTAG 4 cut(s) 86, 332, 903, 932
StyD4I CCNGG 2 cut(s) 340, 406
StyI CCWWGG 2 cut(s) 367, 435
TaaI ACNGT 2 cut(s) 106, 882
TaiI ACGT 2 cut(s) 204, 800
TasI AATT 7 cut(s) 258, 295, 411, 637, 857, 888, 940
TfiI GAWTC 2 cut(s) 353, 779
Tru1I TTAA 3 cut(s) 36, 168, 860
Tru9I TTAA 3 cut(s) 36, 168, 860
TscAI CASTG 1 cut(s) 816
TseI GCWGC 2 cut(s) 584, 801
TspDTI ATGAA 1 cut(s) 489
TspRI CASTG 1 cut(s) 816
Van91I CCANNNNNTGG 2 cut(s) 873, 1001
VneI GTGCAC 1 cut(s) 362
VpaK11BI GGWCC 2 cut(s) 790, 832
VspI ATTAAT 1 cut(s) 860
XapI RAATTY 3 cut(s) 411, 637, 940
XbaI TCTAGA 1 cut(s) 331
XceI RCATGY 2 cut(s) 147, 349
XcmI CCANNNNNNNNNTGG 1 cut(s) 717
XspI CTAG 4 cut(s) 86, 332, 903, 932
ZraI GACGTC 1 cut(s) 202
Zsp2I ATGCAT 1 cut(s) 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.