pycom16g18290

Aldo/keto reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0003777
Physical Location & Seq
Forward (+)
14920916 .. 14924763
3848 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1239 bp
ATGGCATTGGCCTCGTGCTCCACTACCTTTCGCTGCAACCACGCAGCGGCACCGGCACCGGCACCGTGGTCACATCCCAGAACTAGACCCAGAAGCTTACGGGGGCGGAGGAGCTCCATTATCAAAGCAGCCGTCACCACCGATGCCAAGCAAAACCTGAAGACCCCATTGCAGTACAGGAAGCTCGGGGACTCGGACCTTGTAATTAGCGAAATCACTCTCGGAACCATGACATTCGGCGAGCAAAACACAGAGAAAGAAGCTCATGAGATTCTCAGCTATGCATTTGAAAATGGGATTAACGCATTGGACACTGCCGAGGCTTACCCAATTCCCATGAAGAAGGAGACACAGGGGAGCACTGATCGCTACATTGGCAGTTGGCTCAAGTCTCAACCCCGCGATAAGGTTGTTTTGGCAACAAAATTAGCTGGCTATTCAGAGAGGTCAAGTTATTTGCGTGACAATGCCACGGTTTTGCGGGTGGATGCAGCAAACATTAGAGAAAGTGTAGAGAAAAGCCTAAAGCGCCTTGGGACAGATTACATTGATTTAATACAAATTCATTGGCCAGATCGCTATGTGCCATTATTTGGTGGGTATTCATATGATTTTTCAAAATGGAGGCCGAGTGTACCATTCGTGGAGCAACTGAAGGCTTTTCAAGAACTCATTGACGAAGGAAAGGTACGCTACATTGGTGTTTCTAATGAGACTTCATATGGAGTGATGGAATTTGTACATGCAGCTAAAGTTGAAGGGCTACCAAAGATAGTCAGTATCCAAAACAGTTACAGTCTTCTAGTTAGAACTCATTTTGAAATTGACCTTGTTGAAGTCTGCCACCCACAAAATTATAACATTGGCTTGCTGGCTTATTCTCCACTGGCTGGTGGATCACTGAGTGGGAAGTACATAGATAGCACTTCCGAAGCTTCTAAAAAAGGGAGGCTAAACCTCTTCCCCAGCTACATGGAAAGATATAACAAGTCTTCTGCTAGGGAAGCAACAATAAAGTACATCGAGCTAGGCAAGAAGTACGGGCTAACTCCAGTTCAGCTTGCACTTGGATTTGTACGGGACCGTCCATTTGTGACAAGTTCAATCGTTGGTGCAACTTCTGTCACCCAACTGAAGGAAGATATTGATGCTTTTCTGTTGACTGAGAGGCCTTTACCGCAAGAAGTGATGGCTGATATTGAAGAGATTTTCAAGAGATACAAAGACCCTACCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldo_ket_red

Protein Analysis

413

Amino Acids

46.25

Weight (kDa)

8.8

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldo_ket_red PF00248 71 - 403 4.2e-68 Aldo/keto reductase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 858
AccB1I GGYRCC 3 cut(s) 49, 55, 61
AccB7I CCANNNNNTGG 2 cut(s) 593, 890
AccII CGCG 1 cut(s) 402
AciI CCGC 5 cut(s) 47, 106, 400, 481, 1178
AclWI GGATC 1 cut(s) 904
AcoI YGGCCR 1 cut(s) 569
AcsI RAATTY 2 cut(s) 561, 734
AcuI CTGAAG 3 cut(s) 179, 674, 1154
AdeI CACNNNGTG 1 cut(s) 905
AfaI GTAC 8 cut(s) 176, 636, 690, 741, 914, 1019, 1040, 1077
AfiI CCNNNNNNNGG 5 cut(s) 46, 406, 593, 890, 1135
AgsI TTSAA 9 cut(s) 290, 618, 665, 758, 821, 836, 1104, 1202, 1213
Alw21I GWGCWC 3 cut(s) 20, 116, 362
Alw26I GTCTC 3 cut(s) 341, 396, 707
AlwI GGATC 1 cut(s) 904
Ama87I CYCGRG 1 cut(s) 185
AoxI GGCC 4 cut(s) 9, 569, 626, 1169
ApeKI GCWGC 5 cut(s) 33, 44, 128, 491, 746
ApoI RAATTY 2 cut(s) 561, 734
ArsI GACNNNNNNTTYG 4 cut(s) 117, 149, 1073, 1105
AspLEI GCGC 1 cut(s) 531
AspS9I GGNCC 2 cut(s) 196, 1081
AsuHPI GGTGA 2 cut(s) 127, 1117
AvaI CYCGRG 1 cut(s) 185
AvaII GGWCC 2 cut(s) 196, 1081
BalI TGGCCA 1 cut(s) 571
BanI GGYRCC 3 cut(s) 49, 55, 61
BanII GRGCYC 1 cut(s) 116
BarI GAAGNNNNNNTAC 2 cut(s) 672, 704
BauI CACGAG 1 cut(s) 13
BbsI GAAGAC 3 cut(s) 167, 791, 984
Bbv12I GWGCWC 3 cut(s) 20, 116, 362
BbvI GCAGC 5 cut(s) 20, 56, 140, 503, 758
BccI CCATC 2 cut(s) 724, 1183
BceAI ACGGC 1 cut(s) 116
BcgI CGANNNNNNTGC 1 cut(s) 28
BciVI GTATCC 1 cut(s) 791
BcoDI GTCTC 3 cut(s) 341, 396, 707
BfaI CTAG 4 cut(s) 84, 803, 999, 1028
BfoI RGCGCY 1 cut(s) 532
BfuI GTATCC 1 cut(s) 791
BisI GCNGC 6 cut(s) 34, 45, 48, 129, 492, 747
BlsI GCNGC 6 cut(s) 35, 46, 49, 130, 493, 748
Bme18I GGWCC 2 cut(s) 196, 1081
BmeT110I CYCGRG 1 cut(s) 185
BmgT120I GGNCC 2 cut(s) 196, 1081
BmiI GGNNCC 5 cut(s) 51, 57, 63, 226, 1082
BmsI GCATC 3 cut(s) 133, 478, 1138
BpiI GAAGAC 3 cut(s) 167, 791, 984
BpmI CTGGAG 1 cut(s) 1035
BpuEI CTTGAG 1 cut(s) 371
BsaJI CCNNGG 4 cut(s) 65, 318, 471, 532
BsaXI ACNNNNNCTCC 2 cut(s) 616, 646
Bsc4I CCNNNNNNNGG 5 cut(s) 46, 406, 593, 890, 1135
Bse118I RCCGGY 2 cut(s) 52, 58
Bse1I ACTGG 2 cut(s) 891, 1052
Bse3DI GCAATG 1 cut(s) 167
BseDI CCNNGG 4 cut(s) 65, 318, 471, 532
BseGI GGATG 2 cut(s) 73, 493
BseLI CCNNNNNNNGG 5 cut(s) 46, 406, 593, 890, 1135
BseMI GCAATG 1 cut(s) 167
BseMII CTCAG 3 cut(s) 289, 893, 1155
BseNI ACTGG 2 cut(s) 891, 1052
BseRI GAGGAG 1 cut(s) 124
BseXI GCAGC 5 cut(s) 20, 56, 140, 503, 758
BseYI CCCAGC 1 cut(s) 965
Bsh1236I CGCG 1 cut(s) 402
BshFI GGCC 4 cut(s) 11, 571, 628, 1171
BshNI GGYRCC 3 cut(s) 49, 55, 61
BsiHKAI GWGCWC 3 cut(s) 20, 116, 362
BsiHKCI CYCGRG 1 cut(s) 185
BsiSI CCGG 2 cut(s) 53, 59
BslFI GGGAC 3 cut(s) 203, 550, 1094
BslI CCNNNNNNNGG 5 cut(s) 46, 406, 593, 890, 1135
BsmAI GTCTC 3 cut(s) 341, 396, 707
BsmFI GGGAC 3 cut(s) 203, 550, 1094
BsnI GGCC 4 cut(s) 11, 571, 628, 1171
BsoBI CYCGRG 1 cut(s) 185
Bsp1286I GDGCHC 3 cut(s) 20, 116, 362
Bsp1407I TGTACA 1 cut(s) 739
Bsp143I GATC 3 cut(s) 364, 574, 896
BspACI CCGC 5 cut(s) 47, 106, 400, 481, 1178
BspANI GGCC 4 cut(s) 11, 571, 628, 1171
BspCNI CTCAG 3 cut(s) 288, 894, 1156
BspFNI CGCG 1 cut(s) 402
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 5 cut(s) 51, 57, 63, 226, 1082
BspPI GGATC 1 cut(s) 904
BspT107I GGYRCC 3 cut(s) 49, 55, 61
BsrDI GCAATG 1 cut(s) 167
BsrFI RCCGGY 2 cut(s) 52, 58
BsrGI TGTACA 1 cut(s) 739
BsrI ACTGG 2 cut(s) 891, 1052
BssAI RCCGGY 2 cut(s) 52, 58
BssECI CCNNGG 4 cut(s) 65, 318, 471, 532
BssMI GATC 3 cut(s) 364, 574, 896
BssSI CACGAG 1 cut(s) 13
BssT1I CCWWGG 1 cut(s) 532
Bst2BI CACGAG 1 cut(s) 13
Bst4CI ACNGT 5 cut(s) 66, 475, 791, 797, 1085
Bst6I CTCTTC 2 cut(s) 965, 1197
BstAUI TGTACA 1 cut(s) 739
BstC8I GCNNGC 5 cut(s) 242, 433, 869, 873, 1062
BstDEI CTNAG 3 cut(s) 275, 902, 1164
BstDSI CCRYGG 2 cut(s) 65, 471
BstF5I GGATG 2 cut(s) 73, 493
BstFNI CGCG 1 cut(s) 402
BstH2I RGCGCY 1 cut(s) 532
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 3 cut(s) 367, 577, 899
BstMAI GTCTC 3 cut(s) 341, 396, 707
BstMBI GATC 3 cut(s) 364, 574, 896
BstMWI GCNNNNNNNGC 6 cut(s) 53, 366, 375, 528, 1004, 1177
BstNSI RCATGY 1 cut(s) 746
BstUI CGCG 1 cut(s) 402
BstV1I GCAGC 5 cut(s) 20, 56, 140, 503, 758
BstV2I GAAGAC 3 cut(s) 167, 791, 984
BstXI CCANNNNNNTGG 1 cut(s) 973
BsuI GTATCC 1 cut(s) 791
BsuRI GGCC 4 cut(s) 11, 571, 628, 1171
BtgI CCRYGG 2 cut(s) 65, 471
BtsCI GGATG 2 cut(s) 73, 493
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 4 cut(s) 312, 360, 884, 899
Cac8I GCNNGC 5 cut(s) 242, 433, 869, 873, 1062
CciI TCATGA 1 cut(s) 265
CfoI GCGC 1 cut(s) 531
Cfr10I RCCGGY 2 cut(s) 52, 58
Cfr13I GGNCC 2 cut(s) 196, 1081
Csp6I GTAC 8 cut(s) 175, 635, 689, 740, 913, 1018, 1039, 1076
CviAII CATG 5 cut(s) 229, 266, 337, 743, 973
CviQI GTAC 8 cut(s) 175, 635, 689, 740, 913, 1018, 1039, 1076
DdeI CTNAG 3 cut(s) 275, 902, 1164
DpnI GATC 3 cut(s) 366, 576, 898
DpnII GATC 3 cut(s) 364, 574, 896
DraIII CACNNNGTG 1 cut(s) 905
EaeI YGGCCR 1 cut(s) 569
Eam1104I CTCTTC 2 cut(s) 965, 1197
EarI CTCTTC 2 cut(s) 965, 1197
EciI GGCGGA 1 cut(s) 121
Ecl136II GAGCTC 1 cut(s) 114
Eco130I CCWWGG 1 cut(s) 532
Eco147I AGGCCT 1 cut(s) 1171
Eco24I GRGCYC 1 cut(s) 116
Eco47I GGWCC 2 cut(s) 196, 1081
Eco53kI GAGCTC 1 cut(s) 114
Eco57I CTGAAG 3 cut(s) 179, 674, 1154
Eco88I CYCGRG 1 cut(s) 185
EcoICRI GAGCTC 1 cut(s) 114
EcoT14I CCWWGG 1 cut(s) 532
EcoT22I ATGCAT 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 116
ErhI CCWWGG 1 cut(s) 532
FaeI CATG 5 cut(s) 232, 269, 340, 746, 976
FaqI GGGAC 3 cut(s) 203, 550, 1094
FatI CATG 5 cut(s) 228, 265, 336, 742, 972
FauI CCCGC 2 cut(s) 407, 474
FauNDI CATATG 2 cut(s) 607, 721
Fnu4HI GCNGC 6 cut(s) 34, 45, 48, 129, 492, 747
FokI GGATG 2 cut(s) 60, 500
FriOI GRGCYC 1 cut(s) 116
Fsp4HI GCNGC 6 cut(s) 34, 45, 48, 129, 492, 747
FspBI CTAG 4 cut(s) 84, 803, 999, 1028
GlaI GCGC 1 cut(s) 530
GluI GCNGC 6 cut(s) 34, 45, 48, 129, 492, 747
GsaI CCCAGC 1 cut(s) 969
GsuI CTGGAG 1 cut(s) 1035
HaeII RGCGCY 1 cut(s) 532
HaeIII GGCC 4 cut(s) 11, 571, 628, 1171
HapII CCGG 2 cut(s) 53, 59
HhaI GCGC 1 cut(s) 531
Hin1II CATG 5 cut(s) 232, 269, 340, 746, 976
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HincII GTYRAC 1 cut(s) 1161
HindII GTYRAC 1 cut(s) 1161
HindIII AAGCTT 2 cut(s) 94, 933
HinfI GANTC 2 cut(s) 191, 271
HpaII CCGG 2 cut(s) 53, 59
HphI GGTGA 2 cut(s) 127, 1117
Hpy166II GTNNAC 2 cut(s) 635, 1161
Hpy188I TCNGA 4 cut(s) 196, 224, 442, 931
Hpy188III TCNNGA 3 cut(s) 266, 665, 1213
Hpy8I GTNNAC 2 cut(s) 635, 1161
HpyAV CCTTC 5 cut(s) 337, 649, 674, 752, 1129
HpyCH4III ACNGT 5 cut(s) 66, 475, 791, 797, 1085
HpyCH4V TGCA 7 cut(s) 36, 172, 284, 491, 746, 1064, 1115
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 366, 375, 528, 1004, 1177
HpyF3I CTNAG 3 cut(s) 275, 902, 1164
Hsp92II CATG 5 cut(s) 232, 269, 340, 746, 976
HspAI GCGC 1 cut(s) 529
Kzo9I GATC 3 cut(s) 364, 574, 896
LmnI GCTCC 5 cut(s) 23, 111, 119, 357, 646
Lsp1109I GCAGC 5 cut(s) 20, 56, 140, 503, 758
LweI GCATC 3 cut(s) 133, 478, 1138
MaeI CTAG 4 cut(s) 84, 803, 999, 1028
MaeIII GTNAC 6 cut(s) 69, 133, 461, 791, 1093, 1123
MalI GATC 3 cut(s) 366, 576, 898
MboI GATC 3 cut(s) 364, 574, 896
MboII GAAGA 7 cut(s) 172, 352, 791, 952, 984, 1151, 1214
MhlI GDGCHC 3 cut(s) 20, 116, 362
MlsI TGGCCA 1 cut(s) 571
MluCI AATT 7 cut(s) 204, 330, 425, 561, 734, 822, 853
MluNI TGGCCA 1 cut(s) 571
MlyI GAGTC 1 cut(s) 185
MnlI CCTC 8 cut(s) 22, 102, 313, 438, 618, 942, 968, 1161
Mox20I TGGCCA 1 cut(s) 571
Mph1103I ATGCAT 1 cut(s) 286
MscI TGGCCA 1 cut(s) 571
MseI TTAA 2 cut(s) 300, 554
Msp20I TGGCCA 1 cut(s) 571
MspA1I CMGCKG 1 cut(s) 47
MspI CCGG 2 cut(s) 53, 59
MvnI CGCG 1 cut(s) 402
MwoI GCNNNNNNNGC 6 cut(s) 53, 366, 375, 528, 1004, 1177
NdeI CATATG 2 cut(s) 607, 721
NdeII GATC 3 cut(s) 364, 574, 896
NlaIII CATG 5 cut(s) 232, 269, 340, 746, 976
NlaIV GGNNCC 5 cut(s) 51, 57, 63, 226, 1082
NmeAIII GCCGAG 2 cut(s) 343, 654
NmuCI GTSAC 5 cut(s) 69, 133, 461, 1093, 1123
NsiI ATGCAT 1 cut(s) 286
NspI RCATGY 1 cut(s) 746
PagI TCATGA 1 cut(s) 265
PceI AGGCCT 1 cut(s) 1171
PfeI GAWTC 1 cut(s) 271
PflMI CCANNNNNTGG 2 cut(s) 593, 890
PkrI GCNGC 6 cut(s) 35, 46, 49, 130, 493, 748
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
PsiI TTATAA 1 cut(s) 858
Psp124BI GAGCTC 1 cut(s) 116
PspFI CCCAGC 1 cut(s) 965
PspN4I GGNNCC 5 cut(s) 51, 57, 63, 226, 1082
PspPI GGNCC 2 cut(s) 196, 1081
RsaI GTAC 8 cut(s) 176, 636, 690, 741, 914, 1019, 1040, 1077
RsaNI GTAC 8 cut(s) 175, 635, 689, 740, 913, 1018, 1039, 1076
SacI GAGCTC 1 cut(s) 116
SaqAI TTAA 2 cut(s) 300, 554
SatI GCNGC 6 cut(s) 34, 45, 48, 129, 492, 747
Sau3AI GATC 3 cut(s) 364, 574, 896
Sau96I GGNCC 2 cut(s) 196, 1081
SchI GAGTC 1 cut(s) 185
SduI GDGCHC 3 cut(s) 20, 116, 362
SfaNI GCATC 3 cut(s) 133, 478, 1138
SinI GGWCC 2 cut(s) 196, 1081
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 7 cut(s) 204, 330, 425, 561, 734, 822, 853
SseBI AGGCCT 1 cut(s) 1171
SsiI CCGC 5 cut(s) 47, 106, 400, 481, 1178
SspMI CTAG 4 cut(s) 84, 803, 999, 1028
SstI GAGCTC 1 cut(s) 116
StuI AGGCCT 1 cut(s) 1171
StyI CCWWGG 1 cut(s) 532
TaaI ACNGT 5 cut(s) 66, 475, 791, 797, 1085
TaqI TCGA 1 cut(s) 1023
TasI AATT 7 cut(s) 204, 330, 425, 561, 734, 822, 853
TatI WGTACW 4 cut(s) 174, 739, 912, 1017
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 1 cut(s) 271
Tru1I TTAA 2 cut(s) 300, 554
Tru9I TTAA 2 cut(s) 300, 554
TscAI CASTG 4 cut(s) 319, 367, 891, 906
TseFI GTSAC 5 cut(s) 69, 133, 461, 1093, 1123
TseI GCWGC 5 cut(s) 33, 44, 128, 491, 746
Tsp45I GTSAC 5 cut(s) 69, 133, 461, 1093, 1123
TspDTI ATGAA 4 cut(s) 353, 554, 594, 708
TspRI CASTG 4 cut(s) 319, 367, 891, 906
Van91I CCANNNNNTGG 2 cut(s) 593, 890
VpaK11BI GGWCC 2 cut(s) 196, 1081
XapI RAATTY 2 cut(s) 561, 734
XceI RCATGY 1 cut(s) 746
XspI CTAG 4 cut(s) 84, 803, 999, 1028
Zsp2I ATGCAT 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.