pycom16g19960

Biotin carboxylase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004128
Physical Location & Seq
Reverse (-)
17416693 .. 17423302
6610 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1812 bp
ATGCTCTCCTCCGTCAGCCGTCCTCCACTCCTCTTCCTCTCATCACCTCCCAGCTCGCCCCACTCCTTCTTCATTGCCACGGTCAACAGCAATAGTAACCTAAAACCATTCAGTGACTGGGATATTTGGTGTAACACATATCGCTTTGTTTTGAAAATGGATGCCATAATGCCCATCTGCAAGACTGTCACGCCAACTCCGGGCTTATTTTTTGGGAGAACTAGAGAGATCCGGAGCTCCCAATGTAGCTTTATGGTGGGAAATAAAGTCAACTTTTTAAGGCAGAGAGCTCAGGGTGCTCAAGTTAGTATTAAATCCAGGAGGCATGGGGGAGCTCTTCATGCTACATGTCGTGCTGAAAAAATTCTGGTGGCAAATAGAGGGGAAATTGCCGTCCGCGTTATTCGAACAGCCCATGAGATGGGAATACCTTGTGTGGCTGTTTACTCAACAAGAGACAAGGATGCACTTCATGTGAAATTGGCTGATGAATCAGTTTGCATTGGTGAAGCAGCAAGCAGTCAATCGTACTTAGTTGTTCCAAACGTTTTATCTGCTGCCATCAGTCGGAAATGTACAATGTTGCATCCGGGATATGGTTTCCTTTCTGAGAATGCATCATTTGTTGAAATGTGCAAAGAACATGGAATCAATTTTATTGGGCCTAAGCCCGAAAGCATCCGTGTTATGGGTGATAAATCAACTGCCAGAAACACAATGAAGAAAGCAGGTGTTCCAACTGTACCAGGAAGTGATGGATTACTACAGAGCACAGAGGAAGGAATCAGGCTTGCGGATGAGATTGGTTTTCCTGTTATGATCAAAGCCACAGCAGGAGGTGGAGGACGTGGTATGCGTCTTGCTAAAGAACCTGAGGAGTTTGTGAAGTTGTTACAGCAAGCAAAGAGTGAGGCTGCAGCTGCTTTTGGAAATGATGGAGTTTATTTGGAAAAGTACATCCAAAATCCTAGACACATTGAGTTTCAGGTTCTTGCAGATAAATATGGCAGTGTTATTCACTTTGGAGAGCGGGATTGCAGCATCCAGAGAAGGAACCAAAAGCTTCTGGAAGAAGCACCTTCCCCAGCATTGACCCCAGAACTGCGGAAAGCTATGGGTGATGCAGCAGTTGCAGCAGCAGCATCGATTGGCTACATTGGTGTTGGAACCGTTGAATTCCTTTTGGATGAAAGAGGTTCCTTTTACTTCATGGAAATGAACACTCGGATTCAGGTTGAGCATCCTGTGACAGAAATGATTTCCTCTGTTGACTTGATTGAAGAACAAATTCGTGTGGCTATGGGTGAAAAACTCCGATACACACAGGAAGATATAGTGCTCAGAGGACATTCAATTGAATGCCGTATCAATGCAGAAGACGCTTTTAAAGGATTCCGACCTGGGCCAGGAAGAATAACAGCCTACTTACCATCTGGAGGTCCCTTTGTTAGAATGGATAGCCATGTTTATCCTGATTATGTGGTTCCTCCAAATTACGATTCCCTTCTTGGAAAGCTTATTGTTTGGGCACCAACAAGAGAAAAGGCAATTGCGCGAATGAAGAGGGCTCTTGACGACACTGTCATAACAGGGGTTCCCACAACCATTGAATACCATAAACTTATCCTTGATATAGAGGATTTCAAAAATGGCAACGTCGATACTGCTTTTATTCCAAAGCATGAAGAGGAGCTACAAGCGGTAAGAACCCCAACATTTGGTGCCTGCAGCCGCTGCCAAGAACTAGCCGGTGCATCTGCTTACATGGATGGCTTTCGAATTGCTACTATCTGGTCTACTCGACAACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212
Pfam Domains
Protein Families
Biotin_carb_C Biotin_carb_N CPSase_L_D2

Protein Analysis

604

Amino Acids

66.53

Weight (kDa)

8.34

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Biotin_carb_N PF00289 120 - 227 3.6e-42 Biotin carboxylase, N-terminal domain
CPSase_L_D2 PF02786 232 - 439 9.5e-81 Carbamoyl-phosphate synthase L chain, ATP binding domain
ATP-grasp PF02222 254 - 409 2.3e-10 ATP-grasp domain
Dala_Dala_lig_C PF07478 263 - 408 2.1e-06 D-ala D-ala ligase C-terminus
Biotin_carb_C PF02785 453 - 559 2.5e-38 Biotin carboxylase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 719
AasI GACNNNNNNGTC 1 cut(s) 1580
Acc36I ACCTGC 1 cut(s) 719
AccB1I GGYRCC 2 cut(s) 1528, 1721
AccB7I CCANNNNNTGG 2 cut(s) 421, 1718
AccBSI CCGCTC 1 cut(s) 1030
AccI GTMKAC 1 cut(s) 1796
AccII CGCG 2 cut(s) 399, 1555
AccIII TCCGGA 1 cut(s) 231
AciI CCGC 6 cut(s) 397, 794, 1030, 1105, 1700, 1732
AclI AACGTT 1 cut(s) 546
AclWI GGATC 1 cut(s) 223
AcsI RAATTY 3 cut(s) 363, 1175, 1287
AfaI GTAC 4 cut(s) 530, 577, 744, 956
AfiI CCNNNNNNNGG 8 cut(s) 200, 421, 567, 596, 688, 1406, 1436, 1718
AflIII ACRYGT 1 cut(s) 347
AgsI TTSAA 8 cut(s) 154, 629, 1175, 1280, 1353, 1358, 1610, 1645
AjiI CACGTC 1 cut(s) 848
AjnI CCWGG 4 cut(s) 317, 745, 1399, 1405
AjuI GAANNNNNNNTTGG 2 cut(s) 1483, 1515
Alw21I GWGCWC 6 cut(s) 239, 292, 301, 337, 773, 1341
Alw26I GTCTC 1 cut(s) 450
AlwI GGATC 1 cut(s) 223
AlwNI CAGNNNCTG 2 cut(s) 117, 1734
Aor13HI TCCGGA 1 cut(s) 231
AoxI GGCC 2 cut(s) 662, 1403
ApoI RAATTY 3 cut(s) 363, 1175, 1287
Asp700I GAANNNNTTC 2 cut(s) 363, 1287
AspLEI GCGC 1 cut(s) 1555
AspS9I GGNCC 3 cut(s) 662, 1403, 1439
AsuC2I CCSGG 2 cut(s) 201, 591
AsuHPI GGTGA 5 cut(s) 36, 518, 704, 1130, 1316
AsuII TTCGAA 2 cut(s) 406, 1777
AvaII GGWCC 1 cut(s) 1439
AxyI CCTNAGG 1 cut(s) 873
BaeGI GKGCMC 1 cut(s) 1531
BanI GGYRCC 2 cut(s) 1528, 1721
BanII GRGCYC 4 cut(s) 239, 292, 337, 1570
BarI GAAGNNNNNNTAC 2 cut(s) 1677, 1709
BbsI GAAGAC 1 cut(s) 1383
Bbv12I GWGCWC 6 cut(s) 239, 292, 301, 337, 773, 1341
BccI CCATC 7 cut(s) 182, 415, 569, 749, 929, 1438, 1763
BceAI ACGGC 3 cut(s) 3, 377, 1347
BcgI CGANNNNNNTGC 2 cut(s) 1125, 1159
BciT130I CCWGG 4 cut(s) 319, 747, 1401, 1407
BclI TGATCA 1 cut(s) 819
BcnI CCSGG 2 cut(s) 201, 591
BcoDI GTCTC 1 cut(s) 450
BfaI CTAG 3 cut(s) 222, 969, 1745
BfmI CTRYAG 3 cut(s) 764, 915, 1726
BfuAI ACCTGC 1 cut(s) 719
Bme1390I CCNGG 6 cut(s) 201, 319, 591, 747, 1401, 1407
Bme18I GGWCC 1 cut(s) 1439
BmgBI CACGTC 1 cut(s) 848
BmgT120I GGNCC 3 cut(s) 662, 1403, 1439
BmiI GGNNCC 8 cut(s) 1055, 1168, 1198, 1441, 1485, 1530, 1596, 1723
BmrFI CCNGG 6 cut(s) 201, 319, 591, 747, 1401, 1407
BmrI ACTGGG 1 cut(s) 127
BmuI ACTGGG 1 cut(s) 127
BpiI GAAGAC 1 cut(s) 1383
BpmI CTGGAG 1 cut(s) 1455
Bpu10I CCTNAGC 2 cut(s) 291, 666
Bpu14I TTCGAA 2 cut(s) 406, 1777
BpuEI CTTGAG 1 cut(s) 285
BpuMI CCSGG 2 cut(s) 201, 591
Bsa29I ATCGAT 1 cut(s) 1145
BsaJI CCNNGG 2 cut(s) 78, 1400
BsaWI WCCGGW 1 cut(s) 231
BsaXI ACNNNNNCTCC 2 cut(s) 181, 211
Bsc4I CCNNNNNNNGG 8 cut(s) 200, 421, 567, 596, 688, 1406, 1436, 1718
Bse118I RCCGGY 1 cut(s) 1748
Bse1I ACTGG 1 cut(s) 122
Bse21I CCTNAGG 1 cut(s) 873
Bse3DI GCAATG 1 cut(s) 72
BseAI TCCGGA 1 cut(s) 231
BseBI CCWGG 4 cut(s) 319, 747, 1401, 1407
BseCI ATCGAT 1 cut(s) 1145
BseDI CCNNGG 2 cut(s) 78, 1400
BseLI CCNNNNNNNGG 8 cut(s) 200, 421, 567, 596, 688, 1406, 1436, 1718
BseMI GCAATG 1 cut(s) 72
BseMII CTCAG 4 cut(s) 305, 600, 864, 1354
BseNI ACTGG 1 cut(s) 122
BseRI GAGGAG 3 cut(s) 20, 890, 1703
BseSI GKGCMC 1 cut(s) 1531
BseYI CCCAGC 2 cut(s) 50, 1084
Bsh1236I CGCG 2 cut(s) 399, 1555
BshFI GGCC 2 cut(s) 664, 1405
BshNI GGYRCC 2 cut(s) 1528, 1721
BshVI ATCGAT 1 cut(s) 1145
BsiHKAI GWGCWC 6 cut(s) 239, 292, 301, 337, 773, 1341
BsiSI CCGG 4 cut(s) 200, 232, 590, 1749
BslFI GGGAC 1 cut(s) 1425
BslI CCNNNNNNNGG 8 cut(s) 200, 421, 567, 596, 688, 1406, 1436, 1718
BsmAI GTCTC 1 cut(s) 450
BsmFI GGGAC 1 cut(s) 1425
BsmI GAATGC 2 cut(s) 619, 1364
BsnI GGCC 2 cut(s) 664, 1405
Bsp119I TTCGAA 2 cut(s) 406, 1777
Bsp1286I GDGCHC 8 cut(s) 239, 292, 301, 337, 773, 1341, 1531, 1570
Bsp13I TCCGGA 1 cut(s) 231
Bsp1407I TGTACA 1 cut(s) 575
Bsp143I GATC 2 cut(s) 228, 819
BspACI CCGC 6 cut(s) 397, 794, 1030, 1105, 1700, 1732
BspANI GGCC 2 cut(s) 664, 1405
BspCNI CTCAG 4 cut(s) 304, 601, 865, 1353
BspDI ATCGAT 1 cut(s) 1145
BspEI TCCGGA 1 cut(s) 231
BspFNI CGCG 2 cut(s) 399, 1555
BspLI GGNNCC 8 cut(s) 1055, 1168, 1198, 1441, 1485, 1530, 1596, 1723
BspMAI CTGCAG 2 cut(s) 919, 1730
BspMI ACCTGC 1 cut(s) 719
BspPI GGATC 1 cut(s) 223
BspQI GCTCTTC 1 cut(s) 342
BspT104I TTCGAA 2 cut(s) 406, 1777
BspT107I GGYRCC 2 cut(s) 1528, 1721
BsrBI CCGCTC 1 cut(s) 1030
BsrDI GCAATG 1 cut(s) 72
BsrFI RCCGGY 1 cut(s) 1748
BsrGI TGTACA 1 cut(s) 575
BsrI ACTGG 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 1748
BssECI CCNNGG 2 cut(s) 78, 1400
BssMI GATC 2 cut(s) 228, 819
Bst2UI CCWGG 4 cut(s) 319, 747, 1401, 1407
Bst4CI ACNGT 5 cut(s) 82, 187, 742, 1171, 1582
Bst6I CTCTTC 4 cut(s) 38, 342, 1556, 1680
BstAPI GCANNNNNTGC 2 cut(s) 1130, 1734
BstAUI TGTACA 1 cut(s) 575
BstBI TTCGAA 2 cut(s) 406, 1777
BstC8I GCNNGC 5 cut(s) 56, 517, 792, 900, 1726
BstDEI CTNAG 6 cut(s) 291, 532, 609, 666, 873, 1340
BstDSI CCRYGG 1 cut(s) 78
BstENI CCTNNNNNAGG 1 cut(s) 1404
BstFNI CGCG 2 cut(s) 399, 1555
BstHHI GCGC 1 cut(s) 1555
BstKTI GATC 2 cut(s) 231, 822
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 2 cut(s) 228, 819
BstMWI GCNNNNNNNGC 8 cut(s) 296, 341, 920, 1130, 1133, 1139, 1379, 1734
BstNI CCWGG 4 cut(s) 319, 747, 1401, 1407
BstNSI RCATGY 1 cut(s) 351
BstSCI CCNGG 6 cut(s) 199, 317, 589, 745, 1399, 1405
BstSFI CTRYAG 3 cut(s) 764, 915, 1726
BstSLI GKGCMC 1 cut(s) 1531
BstUI CGCG 2 cut(s) 399, 1555
BstV2I GAAGAC 1 cut(s) 1383
BstX2I RGATCY 1 cut(s) 228
BstYI RGATCY 1 cut(s) 228
Bsu15I ATCGAT 1 cut(s) 1145
Bsu36I CCTNAGG 1 cut(s) 873
BsuRI GGCC 2 cut(s) 664, 1405
BsuTUI ATCGAT 1 cut(s) 1145
BtgI CCRYGG 1 cut(s) 78
BtrI CACGTC 1 cut(s) 848
BtsI GCAGTG 1 cut(s) 1015
BtsIMutI CAGTG 3 cut(s) 118, 1015, 1578
BveI ACCTGC 1 cut(s) 719
Cac8I GCNNGC 5 cut(s) 56, 517, 792, 900, 1726
CaiI CAGNNNCTG 2 cut(s) 117, 1734
CfoI GCGC 1 cut(s) 1555
Cfr10I RCCGGY 1 cut(s) 1748
Cfr13I GGNCC 3 cut(s) 662, 1403, 1439
ClaI ATCGAT 1 cut(s) 1145
CseI GACGC 2 cut(s) 845, 1388
Csp6I GTAC 4 cut(s) 529, 576, 743, 955
CspCI CAANNNNNGTGG 2 cut(s) 1588, 1623
CviQI GTAC 4 cut(s) 529, 576, 743, 955
DdeI CTNAG 6 cut(s) 291, 532, 609, 666, 873, 1340
DpnI GATC 2 cut(s) 230, 821
DpnII GATC 2 cut(s) 228, 819
DraI TTTAAA 1 cut(s) 1387
DrdI GACNNNNNNGTC 1 cut(s) 1580
DseDI GACNNNNNNGTC 1 cut(s) 1580
Eam1104I CTCTTC 4 cut(s) 38, 342, 1556, 1680
EarI CTCTTC 4 cut(s) 38, 342, 1556, 1680
Ecl136II GAGCTC 3 cut(s) 237, 290, 335
Eco24I GRGCYC 4 cut(s) 239, 292, 337, 1570
Eco47I GGWCC 1 cut(s) 1439
Eco53kI GAGCTC 3 cut(s) 237, 290, 335
Eco81I CCTNAGG 1 cut(s) 873
EcoICRI GAGCTC 3 cut(s) 237, 290, 335
EcoNI CCTNNNNNAGG 1 cut(s) 1404
EcoO109I RGGNCCY 1 cut(s) 1439
EcoRI GAATTC 1 cut(s) 1175
EcoRII CCWGG 4 cut(s) 317, 745, 1399, 1405
EcoT22I ATGCAT 1 cut(s) 619
EcoT38I GRGCYC 4 cut(s) 239, 292, 337, 1570
FaqI GGGAC 1 cut(s) 1425
FauI CCCGC 1 cut(s) 1023
FbaI TGATCA 1 cut(s) 819
FblI GTMKAC 1 cut(s) 1796
FriOI GRGCYC 4 cut(s) 239, 292, 337, 1570
FspBI CTAG 3 cut(s) 222, 969, 1745
GlaI GCGC 1 cut(s) 1554
GsaI CCCAGC 2 cut(s) 54, 1088
GsuI CTGGAG 1 cut(s) 1455
HaeIII GGCC 2 cut(s) 664, 1405
HapII CCGG 4 cut(s) 200, 232, 590, 1749
HgaI GACGC 2 cut(s) 845, 1388
HhaI GCGC 1 cut(s) 1555
Hin6I GCGC 1 cut(s) 1553
HinP1I GCGC 1 cut(s) 1553
HincII GTYRAC 3 cut(s) 85, 271, 1270
HindII GTYRAC 3 cut(s) 85, 271, 1270
HindIII AAGCTT 2 cut(s) 1061, 1514
HinfI GANTC 6 cut(s) 491, 648, 783, 1228, 1392, 1499
HpaII CCGG 4 cut(s) 200, 232, 590, 1749
HphI GGTGA 5 cut(s) 36, 518, 704, 1130, 1316
Hpy166II GTNNAC 5 cut(s) 85, 271, 445, 1270, 1797
Hpy188I TCNGA 6 cut(s) 570, 610, 1227, 1316, 1343, 1397
Hpy188III TCNNGA 6 cut(s) 232, 1045, 1067, 1434, 1472, 1571
Hpy8I GTNNAC 5 cut(s) 85, 271, 445, 1270, 1797
Hpy99I CGWCG 1 cut(s) 1661
HpyAV CCTTC 5 cut(s) 76, 773, 1044, 1089, 1514
HpyCH4III ACNGT 5 cut(s) 82, 187, 742, 1171, 1582
HpyCH4IV ACGT 3 cut(s) 546, 847, 1656
HpyF10VI GCNNNNNNNGC 8 cut(s) 296, 341, 920, 1130, 1133, 1139, 1379, 1734
HpyF3I CTNAG 6 cut(s) 291, 532, 609, 666, 873, 1340
HpySE526I ACGT 3 cut(s) 546, 847, 1656
HspAI GCGC 1 cut(s) 1553
Kpn2I TCCGGA 1 cut(s) 231
Ksp22I TGATCA 1 cut(s) 819
Kzo9I GATC 2 cut(s) 228, 819
LguI GCTCTTC 1 cut(s) 342
LmnI GCTCC 4 cut(s) 234, 242, 332, 1690
MaeI CTAG 3 cut(s) 222, 969, 1745
MaeII ACGT 3 cut(s) 546, 847, 1656
MaeIII GTNAC 6 cut(s) 95, 113, 131, 187, 891, 1246
MalI GATC 2 cut(s) 230, 821
MbiI CCGCTC 1 cut(s) 1030
MboI GATC 2 cut(s) 228, 819
MfeI CAATTG 2 cut(s) 1353, 1548
MflI RGATCY 1 cut(s) 228
MhlI GDGCHC 8 cut(s) 239, 292, 301, 337, 773, 1341, 1531, 1570
MmeI TCCRAC 4 cut(s) 548, 761, 1144, 1420
Mph1103I ATGCAT 1 cut(s) 619
MroI TCCGGA 1 cut(s) 231
MroXI GAANNNNTTC 2 cut(s) 363, 1287
MseI TTAA 4 cut(s) 278, 312, 1386, 1810
MslI CAYNNNNRTG 1 cut(s) 1214
MspA1I CMGCKG 2 cut(s) 920, 1734
MspI CCGG 4 cut(s) 200, 232, 590, 1749
MspR9I CCNGG 6 cut(s) 201, 319, 591, 747, 1401, 1407
MunI CAATTG 2 cut(s) 1353, 1548
Mva1269I GAATGC 2 cut(s) 619, 1364
MvaI CCWGG 4 cut(s) 319, 747, 1401, 1407
MvnI CGCG 2 cut(s) 399, 1555
MwoI GCNNNNNNNGC 8 cut(s) 296, 341, 920, 1130, 1133, 1139, 1379, 1734
NciI CCSGG 2 cut(s) 201, 591
NdeII GATC 2 cut(s) 228, 819
NlaIV GGNNCC 8 cut(s) 1055, 1168, 1198, 1441, 1485, 1530, 1596, 1723
NmuCI GTSAC 3 cut(s) 113, 187, 1246
NsiI ATGCAT 1 cut(s) 619
NspI RCATGY 1 cut(s) 351
NspV TTCGAA 2 cut(s) 406, 1777
PaqCI CACCTGC 1 cut(s) 719
PciI ACATGT 1 cut(s) 347
PciSI GCTCTTC 1 cut(s) 342
PcsI WCGNNNNNNNCGW 1 cut(s) 853
PctI GAATGC 2 cut(s) 619, 1364
PdmI GAANNNNTTC 2 cut(s) 363, 1287
PfeI GAWTC 6 cut(s) 491, 648, 783, 1228, 1392, 1499
PflFI GACNNNGTC 1 cut(s) 1580
PflMI CCANNNNNTGG 2 cut(s) 421, 1718
PfoI TCCNGGA 2 cut(s) 317, 589
PpuMI RGGWCCY 1 cut(s) 1439
PscI ACATGT 1 cut(s) 347
Psp124BI GAGCTC 3 cut(s) 239, 292, 337
Psp1406I AACGTT 1 cut(s) 546
Psp5II RGGWCCY 1 cut(s) 1439
Psp6I CCWGG 4 cut(s) 317, 745, 1399, 1405
PspFI CCCAGC 2 cut(s) 50, 1084
PspGI CCWGG 4 cut(s) 317, 745, 1399, 1405
PspN4I GGNNCC 8 cut(s) 1055, 1168, 1198, 1441, 1485, 1530, 1596, 1723
PspPI GGNCC 3 cut(s) 662, 1403, 1439
PspPPI RGGWCCY 1 cut(s) 1439
PstI CTGCAG 2 cut(s) 919, 1730
PstNI CAGNNNCTG 2 cut(s) 117, 1734
PsuI RGATCY 1 cut(s) 228
PsyI GACNNNGTC 1 cut(s) 1580
PvuII CAGCTG 1 cut(s) 920
RsaI GTAC 4 cut(s) 530, 577, 744, 956
RsaNI GTAC 4 cut(s) 529, 576, 743, 955
RseI CAYNNNNRTG 1 cut(s) 1214
SacI GAGCTC 3 cut(s) 239, 292, 337
SapI GCTCTTC 1 cut(s) 342
SaqAI TTAA 4 cut(s) 278, 312, 1386, 1810
Sau3AI GATC 2 cut(s) 228, 819
Sau96I GGNCC 3 cut(s) 662, 1403, 1439
ScrFI CCNGG 6 cut(s) 201, 319, 591, 747, 1401, 1407
SduI GDGCHC 8 cut(s) 239, 292, 301, 337, 773, 1341, 1531, 1570
SfcI CTRYAG 3 cut(s) 764, 915, 1726
SfuI TTCGAA 2 cut(s) 406, 1777
SinI GGWCC 1 cut(s) 1439
SmiMI CAYNNNNRTG 1 cut(s) 1214
SmlI CTYRAG 1 cut(s) 300
SmoI CTYRAG 1 cut(s) 300
SsiI CCGC 6 cut(s) 397, 794, 1030, 1105, 1700, 1732
SspMI CTAG 3 cut(s) 222, 969, 1745
SstI GAGCTC 3 cut(s) 239, 292, 337
StyD4I CCNGG 6 cut(s) 199, 317, 589, 745, 1399, 1405
TaaI ACNGT 5 cut(s) 82, 187, 742, 1171, 1582
TaiI ACGT 3 cut(s) 549, 850, 1659
TaqI TCGA 5 cut(s) 406, 1145, 1659, 1777, 1801
TatI WGTACW 2 cut(s) 575, 954
TauI GCSGC 1 cut(s) 1734
TfiI GAWTC 6 cut(s) 491, 648, 783, 1228, 1392, 1499
Tru1I TTAA 4 cut(s) 278, 312, 1386, 1810
Tru9I TTAA 4 cut(s) 278, 312, 1386, 1810
TscAI CASTG 3 cut(s) 118, 1015, 1585
TseFI GTSAC 3 cut(s) 113, 187, 1246
Tsp45I GTSAC 3 cut(s) 113, 187, 1246
TspGWI ACGGA 1 cut(s) 671
TspRI CASTG 3 cut(s) 118, 1015, 1585
Tth111I GACNNNGTC 1 cut(s) 1580
Van91I CCANNNNNTGG 2 cut(s) 421, 1718
VpaK11BI GGWCC 1 cut(s) 1439
XagI CCTNNNNNAGG 1 cut(s) 1404
XapI RAATTY 3 cut(s) 363, 1175, 1287
XceI RCATGY 1 cut(s) 351
XcmI CCANNNNNNNNNTGG 1 cut(s) 114
XmiI GTMKAC 1 cut(s) 1796
XmnI GAANNNNTTC 2 cut(s) 363, 1287
XspI CTAG 3 cut(s) 222, 969, 1745
Zsp2I ATGCAT 1 cut(s) 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.