pycom17g01450

SWI SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002288
Physical Location & Seq
Forward (+)
983507 .. 989010
5504 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3111 bp
ATGGGGAAAAACAAGGTTACGGACGAACTGCTGTCCACCGTCCGATCAATCGTCGACTCGGGATACTCCGACATGGACATTATCAGAGCCCTCCACATGGCCAACAACGACGTCACCGCCGCAATCAACATAATCTTTGACACGCCCAGTTTCAAATTGAAGGAAAGACCACCGGCTTCTCGGAAGAATCCCCAAATTTTGAGCTCCGAGGTCGTAAATTTGAAGCAAAATGGGGGTCAGAAAAGTAATTGCACTTTGGGTACCGAAGGAAATGGGAGTAGTTGCCCCAGTAATTCAGGGGACGACGTGGTTGAAGAAGTCGCGGTTGCACGGTGCGAGAGCTCGGCTGGGAGCGAGTGGTGGTTTGTGGGCAGCAGTGAAGTTTCGGGGTTATCAACGTGTAAAGGTAGGAGGCTTAGCCCTGGAGACGAAGTGGAATTTACTTTTCCAACAAAGAGCAGTTCAACATCTCCGTCACCCGGCAAGGTTTTTGGGAGGGGACGGCAGGCAGCTGCTTGTTCGGAGATTGTGAGGTTTTCAACGAAAGATTCAGGAGAGATTGGTAGAATTCCGAAGGAATGGGCTCGGTGTTTATTGCCACTTGTGAGGGATAAGAAGGTTAGAATTGAGGGGCATTGCAAATCTGCTCCTGATGTGTTGAGCATTATGGACACCATTCTTTTGTCATTAAGTGTGCATATCAATAGTTCAATGTTCCTTAAGCAAAACAAGACCTCACTTAAGGCAGCCAATAATTCCACTGACGAAACAGTGGTTCACCCTCTTCCAACATTGTTCCGGTTGCTAGGACTGACTCCTTTCAAGAAGGCAGAATTTACTCCGAGTGACTTGTACACGAGAAAGCGCCCTTTGGACCCAAAGGATAGCTTTGGACTTTGTGCCCCAGTATTACGTGCCAATAAGCCTAAAATCCCTGGTCAAAATGGAGATGAAGTTGAAAATGAGGAGTCTATTTCAGATGCTGATCTTGAAAACATTGTTGGTATTGGTGACAGCTCTGAGCTAGAGGAAATGGATCCCCCAGGTACACTTCTGTGTGAACTTCGTCCGTACCAAAAGCAGGCTCTTCATTGGATGATCCAACTGGAAAAGGGACACTGCATGGATGAGGGAGCAATGACTCTTCACCCTTGTTGGGAGGCATATCGTCTTGCAGACAAGAGGGACCGTGTTATCTATTTGAATGCATTTTCTGGTGATGCAACAGCAGAATTTCCAAGCACACTTCAAATGGCCAGAGGAGGAATTCTGGCTGATGCTATGGGCTTGGGGAAGACCATTATGACGATATCGCTCCTTCTTACTAATTCAGGACATGGATTATCAGTTAGTTACCCTACAAGCCAGTCTTCCAGTGAAGATATTGAAGTCCCCGACATTTCAGACTACTCATCAAACCTACCAAAGAAGGTCCCAAAATTTTCAGGATTTGATAAGTTGTTGAAGCAAAAAAACACGGTTGGGGATGGTGGTTGTTTGATTATATGTCCTATGACTCTTCTAGGCCAGTGGAAGGCAGAGATTGAAACTCATGCTAAACCTGGGTCTCTATTGGTATATGTTCATTATGGACAAAGTAGACCGAAGGATGCAAAATTTTTAGTTCAAAGTGATGTCGTAATCACTACATATGGAGTGTTAGCCTCTGAATATTCTGCTGAGAATCCAAAGGAAAATGGGGGACTTTACTCGGTTAGTTGGTTTAGAGTGGTTCTTGATGAGGCGCATACCATAAAATCCTCAAAAAGCCAAATTTCCATTGCTGCTGCTGCTTTAGTTGCTGGTCGGCGATGGTGTCTTACTGGCACACCCATACAGAACAACCTGGAGGACGTTTACAGTCTTCTCCGGTTTTTGAGGGTAGAACCTTGGGGAAACTGGGCATGGTGGAATAAACTCATACAAAAACCTTTTGAGGAGGGTGATGAGAGAGGATTAAAGTTGGTTCAGTCTATCTTAAAGCCAATCATGTTAAGGAGAACAAAGTTTAGCACAGACCGCGAAGGCAGGCCAATTTTAGTTCTTCCTCCAGCTGATATTCAGGTGATTTACTGTGAACTCACAGAAGCTGAAAAAGACTTCTACGAGGCCCTCTTTAAAAGATCAAAGGTGAAGTTTGATCAATTTGTTGAGCAAGGACGGGTTCTTCATAATTATGCATCCATATTGGAGTTGCTTTTACGCCTTCGCCAGTGTTGTGATCATCCATTTCTTGTGATGAGCCGAGGTGATACACAAGACTTTTCTGATCTGGATAAGCTAGCTAGACGTTTCCTTAAAGGAAAACAGAATTCTGTGGAAGGGGAAGCCAAAGATCTGCCTTCACGTGCTTATGTTCAAGAAGTTGTAGAAGAAATACGTAAGGGGGAACAGGGAGAGTGTCCAATATGTCTTGAAGCCTTTGAAGATGCAGTGTTAACACCTTGTGCTCACCGCTTATGCCGTGAATGTCTCTTGGCAAGTTGGCGGAACTCAAGTTCTGGTCTCTGTCCTGTTTGTAGGAAGACCATCAGTAAGCAAGATCTTATTACAGCACCCACTGAGAGTCGGTTTCAGGTTGATGTTGAGAAAAACTGGGTGGAATCATCCAAAGTTGTCATTCTTTTGCGTGAACTTGAATCTCTTCGCTCATCAGGCACAAAAAGCATTGTCTTCAGCCAGTGGACGGCTTTTCTGGACCTCTTGCAGGTCCCTCTTTCTAGGAGTAACATTCCATTTCTTCGCCTTGATGGGACTCTGAATCAACAGCAGCGTGAGAAAGTGTTAAAACAGTTTTCAGAAGATAGTGACATCCAGGTGTTGCTAATGTCACTGAAGGCTGGAGGAGTTGGAATAAATCTGACAGCAGCATCAAATGCTTTTGTCTTGGATCCATGGTGGAATCCAGCAGTCGAGGAACAAGCTGTCATGCGTATTCATCGCATTGGACAAACAAAGAGGGTGATGATCAAGCGGTTTATCATGAAGGGAACGGTTGAGGAACGAATGGAAGCAGTGCAAGCACGTAAACAGCGACTGATTTCCGGAGCCTTGACGGACCAAGAAGTTCGAACAGCTCGCATAGAGGAACTAAAGATGCTTTTTACTTGA

Protein Analysis

1037

Amino Acids

115.37

Weight (kDa)

6.95

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_RAD5A PF24559 6 - 50 2.5e-25 DNA repair protein RAD5A UBA domain
HIRAN PF08797 123 - 230 2.5e-17 HIRAN domain
SNF2-rel_dom PF00176 358 - 744 3.2e-76 SNF2-related domain
DEAD PF00270 480 - 618 8.8e-07 DEAD/DEAH box helicase
zf-C3HC4_3 PF13920 798 - 846 9.7e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 800 - 840 7.6e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 800 - 841 2.1e-08 Ring finger domain
zf-C3HC4 PF00097 801 - 840 4.5e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 801 - 838 3.5e-09 RING-type zinc-finger
Prok-RING_4 PF14447 801 - 848 1.8e-06 Prokaryotic RING finger family 4
RING_XB3-XBAT31 PF24921 801 - 845 4e-06 E3 ubiquitin-protein ligase XB3/XBAT31 RING finger
Helicase_C PF00271 872 - 983 6.4e-21 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 114
Acc36I ACCTGC 1 cut(s) 2698
Acc65I GGTACC 1 cut(s) 260
AccB1I GGYRCC 1 cut(s) 260
AccI GTMKAC 2 cut(s) 54, 1600
AccII CGCG 2 cut(s) 323, 2022
AccIII TCCGGA 1 cut(s) 3044
AciI CCGC 7 cut(s) 117, 120, 323, 2020, 2455, 2488, 2974
AclWI GGATC 5 cut(s) 1031, 1044, 1093, 2885, 2898
AcoI YGGCCR 2 cut(s) 99, 1254
AcuI CTGAAG 2 cut(s) 2659, 2855
AcvI CACGTG 1 cut(s) 2348
AcyI GRCGYC 1 cut(s) 111
AdeI CACNNNGTG 1 cut(s) 2447
AfaI GTAC 4 cut(s) 262, 854, 1048, 1073
AfiI CCNNNNNNNGG 7 cut(s) 97, 479, 1081, 1156, 1534, 2686, 2707
AflII CTTAAG 2 cut(s) 719, 740
AflIII ACRYGT 1 cut(s) 398
AjiI CACGTC 1 cut(s) 307
AjnI CCWGG 6 cut(s) 421, 934, 1042, 1561, 1845, 2814
AjuI GAANNNNNNNTTGG 2 cut(s) 984, 1016
AleI CACNNNNGTG 1 cut(s) 1054
AloI GAACNNNNNNTCC 2 cut(s) 2482, 2514
Alw21I GWGCWC 3 cut(s) 206, 344, 2452
Alw26I GTCTC 4 cut(s) 420, 1572, 2477, 2510
AlwI GGATC 5 cut(s) 1031, 1044, 1093, 2885, 2898
AlwNI CAGNNNCTG 3 cut(s) 983, 2090, 3037
Ama87I CYCGRG 1 cut(s) 58
Aor13HI TCCGGA 1 cut(s) 3044
AoxI GGCC 5 cut(s) 99, 1254, 1525, 2030, 2109
ApeKI GCWGC 9 cut(s) 372, 509, 512, 746, 1784, 1787, 1790, 2770, 2867
ArsI GACNNNNNNTTYG 2 cut(s) 220, 252
Asp700I GAANNNNTTC 1 cut(s) 2643
Asp718I GGTACC 1 cut(s) 260
AspLEI GCGC 2 cut(s) 867, 1747
AspS9I GGNCC 7 cut(s) 874, 1186, 1432, 2110, 2698, 2710, 3058
AsuC2I CCSGG 1 cut(s) 480
AsuII TTCGAA 1 cut(s) 3070
AsuNHI GCTAGC 1 cut(s) 2281
AvaI CYCGRG 1 cut(s) 58
AvaII GGWCC 6 cut(s) 874, 1186, 1432, 2698, 2710, 3058
BaeGI GKGCMC 1 cut(s) 904
BalI TGGCCA 2 cut(s) 101, 1256
BamHI GGATCC 2 cut(s) 1036, 2890
BanI GGYRCC 1 cut(s) 260
BanII GRGCYC 4 cut(s) 91, 206, 344, 586
BauI CACGAG 1 cut(s) 856
BbrPI CACGTG 1 cut(s) 2348
BbsI GAAGAC 5 cut(s) 1301, 1362, 1856, 2531, 2665
Bbv12I GWGCWC 3 cut(s) 206, 344, 2452
BbvI GCAGC 9 cut(s) 384, 499, 521, 758, 1771, 1774, 1777, 2782, 2879
BccI CCATC 4 cut(s) 1481, 1806, 2537, 2744
BceAI ACGGC 3 cut(s) 518, 2448, 2703
BciT130I CCWGG 6 cut(s) 423, 936, 1044, 1563, 1847, 2816
BciVI GTATCC 1 cut(s) 56
BclI TGATCA 3 cut(s) 2140, 2221, 2967
BcnI CCSGG 1 cut(s) 480
BcoDI GTCTC 4 cut(s) 420, 1572, 2477, 2510
BfaI CTAG 6 cut(s) 806, 1025, 1523, 2282, 2286, 2721
BfoI RGCGCY 1 cut(s) 868
BfrI CTTAAG 2 cut(s) 719, 740
BfuAI ACCTGC 1 cut(s) 2698
BfuI GTATCC 1 cut(s) 56
BglII AGATCT 2 cut(s) 2335, 2542
BlpI GCTNAGC 1 cut(s) 416
Bme1390I CCNGG 7 cut(s) 423, 480, 936, 1044, 1563, 1847, 2816
Bme18I GGWCC 6 cut(s) 874, 1186, 1432, 2698, 2710, 3058
BmeT110I CYCGRG 1 cut(s) 58
BmgBI CACGTC 1 cut(s) 307
BmgT120I GGNCC 7 cut(s) 874, 1186, 1432, 2110, 2698, 2710, 3058
BmiI GGNNCC 8 cut(s) 262, 876, 1038, 1187, 1434, 2712, 2892, 3049
BmrFI CCNGG 7 cut(s) 423, 480, 936, 1044, 1563, 1847, 2816
BmrI ACTGGG 5 cut(s) 141, 282, 899, 1909, 2605
BmsI GCATC 8 cut(s) 970, 1210, 1267, 1600, 2189, 2419, 2879, 3087
BmtI GCTAGC 1 cut(s) 2285
BmuI ACTGGG 5 cut(s) 141, 282, 899, 1909, 2605
BpiI GAAGAC 5 cut(s) 1301, 1362, 1856, 2531, 2665
BpmI CTGGAG 4 cut(s) 444, 1868, 2034, 2862
Bpu1102I GCTNAGC 1 cut(s) 416
Bpu14I TTCGAA 1 cut(s) 3070
BpuEI CTTGAG 1 cut(s) 2479
BpuMI CCSGG 1 cut(s) 480
BsaAI YACGTR 4 cut(s) 914, 2348, 2381, 3026
BsaBI GATNNNNATC 1 cut(s) 984
BsaHI GRCGYC 1 cut(s) 111
BsaI GGTCTC 2 cut(s) 1572, 2510
BsaJI CCNNGG 8 cut(s) 207, 421, 934, 1042, 1562, 1889, 2245, 2894
BsaWI WCCGGW 3 cut(s) 798, 1869, 3044
BsaXI ACNNNNNCTCC 4 cut(s) 417, 447, 939, 969
Bsc4I CCNNNNNNNGG 7 cut(s) 97, 479, 1081, 1156, 1534, 2686, 2707
Bse118I RCCGGY 1 cut(s) 172
Bse3DI GCAATG 3 cut(s) 634, 1143, 1779
Bse8I GATNNNNATC 1 cut(s) 984
BseAI TCCGGA 1 cut(s) 3044
BseBI CCWGG 6 cut(s) 423, 936, 1044, 1563, 1847, 2816
BseDI CCNNGG 8 cut(s) 207, 421, 934, 1042, 1562, 1889, 2245, 2894
BseGI GGATG 8 cut(s) 1101, 1132, 1492, 1615, 2180, 2224, 2606, 2811
BseJI GATNNNNATC 1 cut(s) 984
BseLI CCNNNNNNNGG 7 cut(s) 97, 479, 1081, 1156, 1534, 2686, 2707
BseMI GCAATG 3 cut(s) 634, 1143, 1779
BseMII CTCAG 3 cut(s) 1011, 1671, 2553
BseRI GAGGAG 4 cut(s) 980, 1275, 1952, 2859
BseSI GKGCMC 1 cut(s) 904
BseXI GCAGC 9 cut(s) 384, 499, 521, 758, 1771, 1774, 1777, 2782, 2879
BseYI CCCAGC 1 cut(s) 347
Bsh1236I CGCG 2 cut(s) 323, 2022
BshFI GGCC 5 cut(s) 101, 1256, 1527, 2032, 2111
BshNI GGYRCC 1 cut(s) 260
BsiHKAI GWGCWC 3 cut(s) 206, 344, 2452
BsiHKCI CYCGRG 1 cut(s) 58
BsiSI CCGG 5 cut(s) 173, 480, 799, 1870, 3045
BslFI GGGAC 9 cut(s) 314, 513, 1128, 1199, 1376, 1418, 1716, 2696, 2767
BslI CCNNNNNNNGG 7 cut(s) 97, 479, 1081, 1156, 1534, 2686, 2707
BsmAI GTCTC 4 cut(s) 420, 1572, 2477, 2510
BsmBI CGTCTC 1 cut(s) 420
BsmFI GGGAC 9 cut(s) 314, 513, 1128, 1199, 1376, 1418, 1716, 2696, 2767
BsmI GAATGC 1 cut(s) 1210
BsnI GGCC 5 cut(s) 101, 1256, 1527, 2032, 2111
Bso31I GGTCTC 2 cut(s) 1572, 2510
BsoBI CYCGRG 1 cut(s) 58
Bsp119I TTCGAA 1 cut(s) 3070
Bsp1286I GDGCHC 6 cut(s) 91, 206, 344, 586, 904, 2452
Bsp13I TCCGGA 1 cut(s) 3044
Bsp1407I TGTACA 1 cut(s) 852
Bsp1720I GCTNAGC 1 cut(s) 416
Bsp19I CCATGG 1 cut(s) 2894
BspACI CCGC 7 cut(s) 117, 120, 323, 2020, 2455, 2488, 2974
BspANI GGCC 5 cut(s) 101, 1256, 1527, 2032, 2111
BspCNI CTCAG 3 cut(s) 1012, 1672, 2554
BspEI TCCGGA 1 cut(s) 3044
BspFNI CGCG 2 cut(s) 323, 2022
BspHI TCATGA 1 cut(s) 2982
BspLI GGNNCC 8 cut(s) 262, 876, 1038, 1187, 1434, 2712, 2892, 3049
BspMI ACCTGC 1 cut(s) 2698
BspOI GCTAGC 1 cut(s) 2285
BspPI GGATC 5 cut(s) 1031, 1044, 1093, 2885, 2898
BspQI GCTCTTC 1 cut(s) 1092
BspT104I TTCGAA 1 cut(s) 3070
BspT107I GGYRCC 1 cut(s) 260
BspTI CTTAAG 2 cut(s) 719, 740
BspTNI GGTCTC 2 cut(s) 1572, 2510
BsrDI GCAATG 3 cut(s) 634, 1143, 1779
BsrFI RCCGGY 1 cut(s) 172
BsrGI TGTACA 1 cut(s) 852
BssAI RCCGGY 1 cut(s) 172
BssECI CCNNGG 8 cut(s) 207, 421, 934, 1042, 1562, 1889, 2245, 2894
BssNI GRCGYC 1 cut(s) 111
BssSI CACGAG 1 cut(s) 856
BssT1I CCWWGG 2 cut(s) 1889, 2894
Bst2BI CACGAG 1 cut(s) 856
Bst2UI CCWGG 6 cut(s) 423, 936, 1044, 1563, 1847, 2816
Bst4CI ACNGT 9 cut(s) 40, 333, 772, 1190, 1480, 1862, 2075, 2793, 2995
Bst6I CTCTTC 5 cut(s) 789, 1092, 1149, 1524, 2649
BstACI GRCGYC 1 cut(s) 111
BstAFI CTTAAG 2 cut(s) 719, 740
BstAPI GCANNNNNTGC 1 cut(s) 2876
BstAUI TGTACA 1 cut(s) 852
BstBAI YACGTR 4 cut(s) 914, 2348, 2381, 3026
BstBI TTCGAA 1 cut(s) 3070
BstC8I GCNNGC 6 cut(s) 507, 1083, 2030, 2283, 3021, 3079
BstDEI CTNAG 4 cut(s) 416, 1020, 1680, 2562
BstDSI CCRYGG 1 cut(s) 2894
BstENI CCTNNNNNAGG 1 cut(s) 2705
BstF5I GGATG 8 cut(s) 1101, 1132, 1492, 1615, 2180, 2224, 2606, 2811
BstFNI CGCG 2 cut(s) 323, 2022
BstH2I RGCGCY 1 cut(s) 868
BstHHI GCGC 2 cut(s) 867, 1747
BstMAI GTCTC 4 cut(s) 420, 1572, 2477, 2510
BstMWI GCNNNNNNNGC 7 cut(s) 1790, 1799, 2019, 2655, 2664, 2876, 3020
BstNI CCWGG 6 cut(s) 423, 936, 1044, 1563, 1847, 2816
BstSCI CCNGG 7 cut(s) 421, 478, 934, 1042, 1561, 1845, 2814
BstSLI GKGCMC 1 cut(s) 904
BstSNI TACGTA 1 cut(s) 2381
BstUI CGCG 2 cut(s) 323, 2022
BstV1I GCAGC 9 cut(s) 384, 499, 521, 758, 1771, 1774, 1777, 2782, 2879
BstV2I GAAGAC 5 cut(s) 1301, 1362, 1856, 2531, 2665
BstX2I RGATCY 4 cut(s) 1036, 2335, 2542, 2890
BstYI RGATCY 4 cut(s) 1036, 2335, 2542, 2890
BsuI GTATCC 1 cut(s) 56
BsuRI GGCC 5 cut(s) 101, 1256, 1527, 2032, 2111
BtgI CCRYGG 1 cut(s) 2894
BtgZI GCGATG 2 cut(s) 1825, 2924
BtrI CACGTC 1 cut(s) 307
BtsCI GGATG 8 cut(s) 1101, 1132, 1492, 1615, 2180, 2224, 2606, 2811
BtsI GCAGTG 4 cut(s) 382, 1117, 2439, 3021
BveI ACCTGC 1 cut(s) 2698
Cac8I GCNNGC 6 cut(s) 507, 1083, 2030, 2283, 3021, 3079
CaiI CAGNNNCTG 3 cut(s) 983, 2090, 3037
CciI TCATGA 1 cut(s) 2982
CfoI GCGC 2 cut(s) 867, 1747
Cfr10I RCCGGY 1 cut(s) 172
Cfr13I GGNCC 7 cut(s) 874, 1186, 1432, 2110, 2698, 2710, 3058
Csp6I GTAC 4 cut(s) 261, 853, 1047, 1072
CviQI GTAC 4 cut(s) 261, 853, 1047, 1072
DdeI CTNAG 4 cut(s) 416, 1020, 1680, 2562
DraI TTTAAA 1 cut(s) 2119
DraIII CACNNNGTG 1 cut(s) 2447
EaeI YGGCCR 2 cut(s) 99, 1254
Eam1104I CTCTTC 5 cut(s) 789, 1092, 1149, 1524, 2649
EarI CTCTTC 5 cut(s) 789, 1092, 1149, 1524, 2649
EciI GGCGGA 1 cut(s) 2503
Ecl136II GAGCTC 2 cut(s) 204, 342
Eco105I TACGTA 1 cut(s) 2381
Eco130I CCWWGG 2 cut(s) 1889, 2894
Eco24I GRGCYC 4 cut(s) 91, 206, 344, 586
Eco31I GGTCTC 2 cut(s) 1572, 2510
Eco32I GATATC 1 cut(s) 1311
Eco47I GGWCC 6 cut(s) 874, 1186, 1432, 2698, 2710, 3058
Eco53kI GAGCTC 2 cut(s) 204, 342
Eco57I CTGAAG 2 cut(s) 2659, 2855
Eco72I CACGTG 1 cut(s) 2348
Eco88I CYCGRG 1 cut(s) 58
EcoICRI GAGCTC 2 cut(s) 204, 342
EcoNI CCTNNNNNAGG 1 cut(s) 2705
EcoO109I RGGNCCY 3 cut(s) 1432, 2110, 2710
EcoRI GAATTC 3 cut(s) 567, 1266, 2311
EcoRII CCWGG 6 cut(s) 421, 934, 1042, 1561, 1845, 2814
EcoRV GATATC 1 cut(s) 1311
EcoT14I CCWWGG 2 cut(s) 1889, 2894
EcoT22I ATGCAT 2 cut(s) 1210, 2182
EcoT38I GRGCYC 4 cut(s) 91, 206, 344, 586
ErhI CCWWGG 2 cut(s) 1889, 2894
Esp3I CGTCTC 1 cut(s) 420
FalI AAGNNNNNCTT 4 cut(s) 872, 904, 1354, 1386
FaqI GGGAC 9 cut(s) 314, 513, 1128, 1199, 1376, 1418, 1716, 2696, 2767
FauNDI CATATG 1 cut(s) 1651
FbaI TGATCA 3 cut(s) 2140, 2221, 2967
FblI GTMKAC 2 cut(s) 54, 1600
FokI GGATG 8 cut(s) 1108, 1139, 1499, 1622, 2167, 2211, 2593, 2798
FriOI GRGCYC 4 cut(s) 91, 206, 344, 586
FspBI CTAG 6 cut(s) 806, 1025, 1523, 2282, 2286, 2721
GlaI GCGC 2 cut(s) 866, 1746
GsaI CCCAGC 1 cut(s) 351
GsuI CTGGAG 4 cut(s) 444, 1868, 2034, 2862
HaeII RGCGCY 1 cut(s) 868
HaeIII GGCC 5 cut(s) 101, 1256, 1527, 2032, 2111
HapII CCGG 5 cut(s) 173, 480, 799, 1870, 3045
HhaI GCGC 2 cut(s) 867, 1747
Hin1I GRCGYC 1 cut(s) 111
Hin6I GCGC 2 cut(s) 865, 1745
HinP1I GCGC 2 cut(s) 865, 1745
HincII GTYRAC 2 cut(s) 55, 2439
HindII GTYRAC 2 cut(s) 55, 2439
HpaI GTTAAC 1 cut(s) 2439
HpaII CCGG 5 cut(s) 173, 480, 799, 1870, 3045
Hpy99I CGWCG 3 cut(s) 56, 113, 308
HpyCH4III ACNGT 9 cut(s) 40, 333, 772, 1190, 1480, 1862, 2075, 2793, 2995
HpyCH4IV ACGT 9 cut(s) 111, 306, 398, 913, 1854, 2290, 2347, 2380, 3025
HpyF10VI GCNNNNNNNGC 7 cut(s) 1790, 1799, 2019, 2655, 2664, 2876, 3020
HpyF3I CTNAG 4 cut(s) 416, 1020, 1680, 2562
HpySE526I ACGT 9 cut(s) 111, 306, 398, 913, 1854, 2290, 2347, 2380, 3025
Hsp92I GRCGYC 1 cut(s) 111
HspAI GCGC 2 cut(s) 865, 1745
Kpn2I TCCGGA 1 cut(s) 3044
KpnI GGTACC 1 cut(s) 264
Ksp22I TGATCA 3 cut(s) 2140, 2221, 2967
KspAI GTTAAC 1 cut(s) 2439
LguI GCTCTTC 1 cut(s) 1092
LmnI GCTCC 6 cut(s) 209, 351, 652, 1133, 1320, 3047
Lsp1109I GCAGC 9 cut(s) 384, 499, 521, 758, 1771, 1774, 1777, 2782, 2879
LweI GCATC 8 cut(s) 970, 1210, 1267, 1600, 2189, 2419, 2879, 3087
MaeI CTAG 6 cut(s) 806, 1025, 1523, 2282, 2286, 2721
MaeII ACGT 9 cut(s) 111, 306, 398, 913, 1854, 2290, 2347, 2380, 3025
MaeIII GTNAC 9 cut(s) 16, 112, 474, 845, 1010, 1352, 2726, 2807, 2829
MflI RGATCY 4 cut(s) 1036, 2335, 2542, 2890
MhlI GDGCHC 6 cut(s) 91, 206, 344, 586, 904, 2452
MlsI TGGCCA 2 cut(s) 101, 1256
MluNI TGGCCA 2 cut(s) 101, 1256
MlyI GAGTC 7 cut(s) 50, 808, 977, 1135, 1510, 2575, 2749
MmeI TCCRAC 5 cut(s) 93, 473, 812, 1126, 2830
Mox20I TGGCCA 2 cut(s) 101, 1256
Mph1103I ATGCAT 2 cut(s) 1210, 2182
MroI TCCGGA 1 cut(s) 3044
MroXI GAANNNNTTC 1 cut(s) 2643
MscI TGGCCA 2 cut(s) 101, 1256
MslI CAYNNNNRTG 3 cut(s) 1054, 2175, 2816
Msp20I TGGCCA 2 cut(s) 101, 1256
MspA1I CMGCKG 2 cut(s) 512, 2054
MspCI CTTAAG 2 cut(s) 719, 740
MspI CCGG 5 cut(s) 173, 480, 799, 1870, 3045
MspR9I CCNGG 7 cut(s) 423, 480, 936, 1044, 1563, 1847, 2816
Mva1269I GAATGC 1 cut(s) 1210
MvaI CCWGG 6 cut(s) 423, 936, 1044, 1563, 1847, 2816
MvnI CGCG 2 cut(s) 323, 2022
MwoI GCNNNNNNNGC 7 cut(s) 1790, 1799, 2019, 2655, 2664, 2876, 3020
NciI CCSGG 1 cut(s) 480
NcoI CCATGG 1 cut(s) 2894
NdeI CATATG 1 cut(s) 1651
NheI GCTAGC 1 cut(s) 2281
NlaIV GGNNCC 8 cut(s) 262, 876, 1038, 1187, 1434, 2712, 2892, 3049
NmeAIII GCCGAG 2 cut(s) 323, 2270
NmuCI GTSAC 6 cut(s) 112, 474, 845, 1010, 2807, 2829
NsiI ATGCAT 2 cut(s) 1210, 2182
NspV TTCGAA 1 cut(s) 3070
OliI CACNNNNGTG 1 cut(s) 1054
PagI TCATGA 1 cut(s) 2982
PciSI GCTCTTC 1 cut(s) 1092
PcsI WCGNNNNNNNCGW 1 cut(s) 3031
PctI GAATGC 1 cut(s) 1210
PdmI GAANNNNTTC 1 cut(s) 2643
PfeI GAWTC 7 cut(s) 187, 548, 1684, 2603, 2639, 2761, 2902
PleI GAGTC 7 cut(s) 50, 808, 976, 1135, 1510, 2574, 2749
PmaCI CACGTG 1 cut(s) 2348
PmlI CACGTG 1 cut(s) 2348
PpsI GAGTC 7 cut(s) 50, 808, 976, 1135, 1510, 2574, 2749
Ppu21I YACGTR 4 cut(s) 914, 2348, 2381, 3026
PpuMI RGGWCCY 2 cut(s) 1432, 2710
Psp124BI GAGCTC 2 cut(s) 206, 344
Psp5II RGGWCCY 2 cut(s) 1432, 2710
Psp6I CCWGG 6 cut(s) 421, 934, 1042, 1561, 1845, 2814
PspCI CACGTG 1 cut(s) 2348
PspFI CCCAGC 1 cut(s) 347
PspGI CCWGG 6 cut(s) 421, 934, 1042, 1561, 1845, 2814
PspN4I GGNNCC 8 cut(s) 262, 876, 1038, 1187, 1434, 2712, 2892, 3049
PspPI GGNCC 7 cut(s) 874, 1186, 1432, 2110, 2698, 2710, 3058
PspPPI RGGWCCY 2 cut(s) 1432, 2710
PstNI CAGNNNCTG 3 cut(s) 983, 2090, 3037
PsuI RGATCY 4 cut(s) 1036, 2335, 2542, 2890
PvuII CAGCTG 2 cut(s) 512, 2054
RsaI GTAC 4 cut(s) 262, 854, 1048, 1073
RsaNI GTAC 4 cut(s) 261, 853, 1047, 1072
RseI CAYNNNNRTG 3 cut(s) 1054, 2175, 2816
SacI GAGCTC 2 cut(s) 206, 344
SalI GTCGAC 1 cut(s) 53
SapI GCTCTTC 1 cut(s) 1092
Sau96I GGNCC 7 cut(s) 874, 1186, 1432, 2110, 2698, 2710, 3058
SchI GAGTC 7 cut(s) 50, 808, 977, 1135, 1510, 2575, 2749
ScrFI CCNGG 7 cut(s) 423, 480, 936, 1044, 1563, 1847, 2816
SduI GDGCHC 6 cut(s) 91, 206, 344, 586, 904, 2452
SfaNI GCATC 8 cut(s) 970, 1210, 1267, 1600, 2189, 2419, 2879, 3087
SfuI TTCGAA 1 cut(s) 3070
SinI GGWCC 6 cut(s) 874, 1186, 1432, 2698, 2710, 3058
SmiMI CAYNNNNRTG 3 cut(s) 1054, 2175, 2816
SmlI CTYRAG 3 cut(s) 719, 740, 2494
SmoI CTYRAG 3 cut(s) 719, 740, 2494
SnaBI TACGTA 1 cut(s) 2381
SsiI CCGC 7 cut(s) 117, 120, 323, 2020, 2455, 2488, 2974
SspI AATATT 1 cut(s) 1673
SspMI CTAG 6 cut(s) 806, 1025, 1523, 2282, 2286, 2721
SstI GAGCTC 2 cut(s) 206, 344
StyD4I CCNGG 7 cut(s) 421, 478, 934, 1042, 1561, 1845, 2814
StyI CCWWGG 2 cut(s) 1889, 2894
TaaI ACNGT 9 cut(s) 40, 333, 772, 1190, 1480, 1862, 2075, 2793, 2995
TaiI ACGT 9 cut(s) 114, 309, 401, 916, 1857, 2293, 2350, 2383, 3028
TaqI TCGA 3 cut(s) 54, 2913, 3070
TaqII GACCGA 1 cut(s) 1618
TatI WGTACW 1 cut(s) 852
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 7 cut(s) 187, 548, 1684, 2603, 2639, 2761, 2902
TseFI GTSAC 6 cut(s) 112, 474, 845, 1010, 2807, 2829
TseI GCWGC 9 cut(s) 372, 509, 512, 746, 1784, 1787, 1790, 2770, 2867
Tsp45I GTSAC 6 cut(s) 112, 474, 845, 1010, 2807, 2829
TspDTI ATGAA 6 cut(s) 966, 1079, 1574, 2159, 2927, 2999
TspGWI ACGGA 4 cut(s) 35, 462, 1059, 3071
Vha464I CTTAAG 2 cut(s) 719, 740
VpaK11BI GGWCC 6 cut(s) 874, 1186, 1432, 2698, 2710, 3058
XagI CCTNNNNNAGG 1 cut(s) 2705
XmiI GTMKAC 2 cut(s) 54, 1600
XmnI GAANNNNTTC 1 cut(s) 2643
XspI CTAG 6 cut(s) 806, 1025, 1523, 2282, 2286, 2721
ZraI GACGTC 1 cut(s) 112
Zsp2I ATGCAT 2 cut(s) 1210, 2182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.