pycom17g04570

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002986
Physical Location & Seq
Reverse (-)
3113565 .. 3116599
3035 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2223 bp
ATGGGTGCCTTAATTAATTCTTTGCTACATACCCACTTTGATTTCGGGAATATGGGTTTTTCTGGTGGGGGTTTTTACTCATATACGCCTCTTTCTCCTAGGTTTTTCACCCTCTCTGTCTCCTCCCTCTTGCTTGCTCTGTTCAGATTTTTCAGCAAGTTCTTCCTCAGAGTTAGTTTTGTTAATGGTGACAGTTCTTGGATGAACAATTATTGTTGTTCTGGCCCTGTTTCTGAAGAAACTGATCAAATGGTATCGAATAATAATCTTGTTGAGACGGAAGCTCTGCCTGGAAAATCGGATTCTTGTTGTCCTAGCTCTGCTTCTGAAGAAACCAATCAAATGGTATCGAATAATCCGGCTGAGCCAGAAGCTCTGCCTGAGAAAGCTGATTCCGAGATTTCGAAAAGCCATGGAGATTCAGAGTTTGGTGAAGCTGATGATGGAGAGGCGTCGAAGCCGAGTTTCGTGTTTAAATTCGAGTACCAGACTAAAGAAATATTAAGCAGAAGCAATAGAGAGTGTAAGTTTATGGCTGATAAGTTTAACCTATTCAAAGAAGAACCAGAATTCACAAGCAGCAGCAGCAGAAAATACGAATTCAAAGCCGAAGAAAGTGTGAGTTGCTTCCTGGAAGAAGCTAAAGTTGCAACCTTTACTGTCGAAGAAGGAGGTGATCATGAACAAATTACTCATGAATTTGCAGGTGAGGAATGCGAGGAAGATGAAGTGTCTGATAAGAAATGGGTGACGGAAGATCCTCATTTGGATGAAGAGCGAGTGGGGAGTCTTGAAAGCAATTCAGATTGTGAGAAAGTTGATTCTCATGAAGTTAAGTTCTTGTCAGAAAAGGACTTTGTTGCTTCAGATTATGAGTGTGCTTCTATCGGCTCTCCAAGTGTGGGGTTTTTGTCAGTAGCAGATTTTGGGGAAAGAAATGACCGGCTTGAGAATTTCGATATGGGGCACGAGTCTGATTGTTTCGACGATGAAGATGAAGATATAAACGAACAAATTCAAGAATCGGAAGAGTTGTACAGCAGCTCGAGTCCAGAAGGGTTTAGTAGCAAAGACGAAAAACCAATTGAGGGTTTCGAAAATGCTGAAACGCCCCAAGTTCGTAGTTTGATACCTAATGCTTCCGAGGATTCAAACACATTGGAATCATTGTGGGAACACCAGGATTTGATAGAACAGTTGAAAATGGAGCTCAAGAAAGTCAGGGCTACTGGCCTGCCTACCATTCTTGAAGAATCCGAGTCCCCGAATATGGATGATTTGAAGCCATGGAAGATCGATGAAAAAGTCCATCAAGGTGATAGAATGGGAGATCTTCAGAACTTCCACAGAAGTTACAGAGAGCGCATGCGAAAATTCGACATTTTGAATTACCAGAAGTTATATGCAATAGTTCACATGATCTTAATTTCTCATGTGTTTTATGAAAAATCCAAATGTGTAGGTTTCTTGCAGTCCAAGGATCCACTTCAGTCGTTTCCGAGCTCCAAGTCCTCAGCTCCGGCAATCCCATCCTTTCTCTCACAGAATTTTTGGGGATGCAAACTGAAAAAGGATTCGGATTCCGACCCAATGGTGAAGTTTATTAGAGAATTGCATAGTGAATTAGAAGTGGTTTATGTGGGGCAGTTGTGCCTTTCTTGGGAGATCCTTCAATGGCAGTATGAGAAGGCCGTCAAGCTTTGGGAATCGGCTCCGTCTGGAATTGGTTCGTACAATGCAGTCGCGGGCGAATTCCAACAGTTCCAAGTGCACTTGCATAGATTTGTAGAGAATGAATGTTATCAAGGGCCTAGGGTTGAAAATTACGTCAAGAATCGATGCATCACGCGCAATCTTCTTCAAGTTCCGGTGATTAGAGGGGACAATTTAAAGGAGAGAAAGAAAGCAAGAAGAAAAGGCAAAGATGATGGTGCAATTACAAGCGACATTCTAGTAGAGATACTCGAAGAATCAGTAAGGGCGATCTGGAGATTCATTCGAGCTGATAAACATGCTAATTGTACGCTTGCATGTCGAAAGAGAAGACCAGGAGAAGAGCTTGAAGAATCGGATTTGAAGCTTTACACAGAGATCCAAACAAATCTTCACAATGTACGTTTCATATCAGACAGTCTAACATGTCATTCCGTATCACGATTTTTCATGTTTCGGTTTGACCTAACGATGAAGTTAACCTGTACTTTGGAATGCAGAAAAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

741

Amino Acids

84.67

Weight (kDa)

4.86

Isoelectric Point (pI)

52.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 540 - 703 1.4e-53 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 695
Acc36I ACCTGC 1 cut(s) 695
AccB1I GGYRCC 1 cut(s) 5
AccII CGCG 2 cut(s) 1745, 1849
AciI CCGC 1 cut(s) 1745
AclWI GGATC 5 cut(s) 752, 1475, 1488, 1660, 2086
AcsI RAATTY 9 cut(s) 476, 569, 599, 698, 952, 1014, 1373, 1546, 1751
AcuI CTGAAG 5 cut(s) 255, 348, 849, 1319, 1472
AcyI GRCGYC 1 cut(s) 452
AfaI GTAC 6 cut(s) 485, 1037, 1733, 2023, 2115, 2200
AfiI CCNNNNNNNGG 4 cut(s) 902, 1088, 1270, 1660
AflIII ACRYGT 1 cut(s) 2138
AjnI CCWGG 4 cut(s) 289, 630, 1179, 2047
AjuI GAANNNNNNNTTGG 2 cut(s) 2088, 2120
Alw21I GWGCWC 3 cut(s) 1212, 1505, 1773
Alw26I GTCTC 2 cut(s) 124, 269
Alw44I GTGCAC 1 cut(s) 1769
AlwI GGATC 5 cut(s) 752, 1475, 1488, 1660, 2086
Ama87I CYCGRG 1 cut(s) 1045
AoxI GGCC 4 cut(s) 223, 1231, 1689, 1808
ApaLI GTGCAC 1 cut(s) 1769
ApeKI GCWGC 4 cut(s) 579, 582, 585, 1041
ApoI RAATTY 9 cut(s) 476, 569, 599, 698, 952, 1014, 1373, 1546, 1751
AseI ATTAAT 1 cut(s) 15
Asp700I GAANNNNTTC 2 cut(s) 1014, 1726
AspA2I CCTAGG 2 cut(s) 98, 1811
AspLEI GCGC 2 cut(s) 1365, 1851
AspS9I GGNCC 2 cut(s) 224, 1808
AsuHPI GGTGA 9 cut(s) 100, 200, 443, 686, 719, 760, 1328, 1606, 1882
AsuII TTCGAA 2 cut(s) 404, 1095
AvaI CYCGRG 1 cut(s) 1045
AvrII CCTAGG 2 cut(s) 98, 1811
BaeGI GKGCMC 2 cut(s) 969, 1773
BamHI GGATCC 1 cut(s) 1480
BanI GGYRCC 1 cut(s) 5
BanII GRGCYC 2 cut(s) 1212, 1505
BauI CACGAG 1 cut(s) 968
BbsI GAAGAC 1 cut(s) 2050
Bbv12I GWGCWC 3 cut(s) 1212, 1505, 1773
BbvCI CCTCAGC 1 cut(s) 1513
BbvI GCAGC 4 cut(s) 591, 594, 597, 1053
BccI CCATC 4 cut(s) 437, 1317, 1537, 1922
BceAI ACGGC 1 cut(s) 1676
BciT130I CCWGG 4 cut(s) 291, 632, 1181, 2049
BclI TGATCA 2 cut(s) 244, 676
BcoDI GTCTC 2 cut(s) 124, 269
BfaI CTAG 4 cut(s) 99, 315, 1812, 1952
BfuAI ACCTGC 1 cut(s) 695
BglII AGATCT 1 cut(s) 1330
BisI GCNGC 4 cut(s) 580, 583, 586, 1042
BlnI CCTAGG 2 cut(s) 98, 1811
BlpI GCTNAGC 1 cut(s) 363
BlsI GCNGC 4 cut(s) 581, 584, 587, 1043
Bme1390I CCNGG 4 cut(s) 291, 632, 1181, 2049
BmeT110I CYCGRG 1 cut(s) 1045
BmgT120I GGNCC 2 cut(s) 224, 1808
BmiI GGNNCC 3 cut(s) 7, 1482, 1713
BmrFI CCNGG 4 cut(s) 291, 632, 1181, 2049
BmsI GCATC 3 cut(s) 1547, 1829, 1851
BpiI GAAGAC 1 cut(s) 2050
BpmI CTGGAG 1 cut(s) 2008
Bpu10I CCTNAGC 1 cut(s) 1513
Bpu1102I GCTNAGC 1 cut(s) 363
Bpu14I TTCGAA 2 cut(s) 404, 1095
BpuEI CTTGAG 2 cut(s) 968, 1196
Bsa29I ATCGAT 2 cut(s) 1296, 1837
BsaHI GRCGYC 1 cut(s) 452
BsaJI CCNNGG 6 cut(s) 98, 412, 1143, 1286, 1476, 1811
BsaWI WCCGGW 1 cut(s) 1867
Bsc4I CCNNNNNNNGG 4 cut(s) 902, 1088, 1270, 1660
Bse118I RCCGGY 1 cut(s) 942
Bse1I ACTGG 1 cut(s) 1234
BseBI CCWGG 4 cut(s) 291, 632, 1181, 2049
BseCI ATCGAT 2 cut(s) 1296, 1837
BseDI CCNNGG 6 cut(s) 98, 412, 1143, 1286, 1476, 1811
BseGI GGATG 5 cut(s) 207, 775, 1279, 1529, 1562
BseLI CCNNNNNNNGG 4 cut(s) 902, 1088, 1270, 1660
BseMII CTCAG 4 cut(s) 181, 354, 372, 1527
BseNI ACTGG 1 cut(s) 1234
BseRI GAGGAG 1 cut(s) 112
BseSI GKGCMC 2 cut(s) 969, 1773
BseXI GCAGC 4 cut(s) 591, 594, 597, 1053
Bsh1236I CGCG 2 cut(s) 1745, 1849
BshFI GGCC 4 cut(s) 225, 1233, 1691, 1810
BshNI GGYRCC 1 cut(s) 5
BshVI ATCGAT 2 cut(s) 1296, 1837
BsiHKAI GWGCWC 3 cut(s) 1212, 1505, 1773
BsiHKCI CYCGRG 1 cut(s) 1045
BsiSI CCGG 4 cut(s) 359, 943, 1520, 1868
BslFI GGGAC 2 cut(s) 1246, 1895
BslI CCNNNNNNNGG 4 cut(s) 902, 1088, 1270, 1660
BsmAI GTCTC 2 cut(s) 124, 269
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 2 cut(s) 1246, 1895
BsmI GAATGC 2 cut(s) 719, 2213
BsnI GGCC 4 cut(s) 225, 1233, 1691, 1810
BsoBI CYCGRG 1 cut(s) 1045
Bsp119I TTCGAA 2 cut(s) 404, 1095
Bsp1286I GDGCHC 4 cut(s) 969, 1212, 1505, 1773
Bsp1407I TGTACA 1 cut(s) 1035
Bsp1720I GCTNAGC 1 cut(s) 363
Bsp19I CCATGG 2 cut(s) 412, 1286
BspACI CCGC 1 cut(s) 1745
BspANI GGCC 4 cut(s) 225, 1233, 1691, 1810
BspCNI CTCAG 4 cut(s) 180, 355, 373, 1526
BspDI ATCGAT 2 cut(s) 1296, 1837
BspFNI CGCG 2 cut(s) 1745, 1849
BspHI TCATGA 3 cut(s) 679, 694, 826
BspLI GGNNCC 3 cut(s) 7, 1482, 1713
BspMI ACCTGC 1 cut(s) 695
BspPI GGATC 5 cut(s) 752, 1475, 1488, 1660, 2086
BspQI GCTCTTC 2 cut(s) 768, 2049
BspT104I TTCGAA 2 cut(s) 404, 1095
BspT107I GGYRCC 1 cut(s) 5
BsrFI RCCGGY 1 cut(s) 942
BsrGI TGTACA 1 cut(s) 1035
BsrI ACTGG 1 cut(s) 1234
BssAI RCCGGY 1 cut(s) 942
BssECI CCNNGG 6 cut(s) 98, 412, 1143, 1286, 1476, 1811
BssNI GRCGYC 1 cut(s) 452
BssSI CACGAG 1 cut(s) 968
BssT1I CCWWGG 5 cut(s) 98, 412, 1286, 1476, 1811
Bst2BI CACGAG 1 cut(s) 968
Bst2UI CCWGG 4 cut(s) 291, 632, 1181, 2049
Bst4CI ACNGT 5 cut(s) 194, 661, 1197, 1761, 2132
Bst6I CTCTTC 3 cut(s) 768, 1023, 2049
BstACI GRCGYC 1 cut(s) 452
BstAUI TGTACA 1 cut(s) 1035
BstBI TTCGAA 2 cut(s) 404, 1095
BstC8I GCNNGC 5 cut(s) 135, 1235, 1367, 1747, 2028
BstDEI CTNAG 4 cut(s) 167, 363, 381, 1513
BstDSI CCRYGG 2 cut(s) 412, 1286
BstF5I GGATG 5 cut(s) 207, 775, 1279, 1529, 1562
BstFNI CGCG 2 cut(s) 1745, 1849
BstHHI GCGC 2 cut(s) 1365, 1851
BstMAI GTCTC 2 cut(s) 124, 269
BstMWI GCNNNNNNNGC 3 cut(s) 585, 647, 1848
BstNI CCWGG 4 cut(s) 291, 632, 1181, 2049
BstNSI RCATGY 4 cut(s) 1369, 2015, 2034, 2142
BstSCI CCNGG 4 cut(s) 289, 630, 1179, 2047
BstSLI GKGCMC 2 cut(s) 969, 1773
BstUI CGCG 2 cut(s) 1745, 1849
BstV1I GCAGC 4 cut(s) 591, 594, 597, 1053
BstV2I GAAGAC 1 cut(s) 2050
BstX2I RGATCY 5 cut(s) 757, 1330, 1480, 1665, 2091
BstXI CCANNNNNNTGG 1 cut(s) 343
BstYI RGATCY 5 cut(s) 757, 1330, 1480, 1665, 2091
Bsu15I ATCGAT 2 cut(s) 1296, 1837
BsuRI GGCC 4 cut(s) 225, 1233, 1691, 1810
BsuTUI ATCGAT 2 cut(s) 1296, 1837
BtgI CCRYGG 2 cut(s) 412, 1286
BtsCI GGATG 5 cut(s) 207, 775, 1279, 1529, 1562
BveI ACCTGC 1 cut(s) 695
Cac8I GCNNGC 5 cut(s) 135, 1235, 1367, 1747, 2028
CciI TCATGA 3 cut(s) 679, 694, 826
CfoI GCGC 2 cut(s) 1365, 1851
Cfr10I RCCGGY 1 cut(s) 942
Cfr13I GGNCC 2 cut(s) 224, 1808
ClaI ATCGAT 2 cut(s) 1296, 1837
CseI GACGC 1 cut(s) 441
Csp6I GTAC 6 cut(s) 484, 1036, 1732, 2022, 2114, 2199
CviQI GTAC 6 cut(s) 484, 1036, 1732, 2022, 2114, 2199
DdeI CTNAG 4 cut(s) 167, 363, 381, 1513
DraI TTTAAA 2 cut(s) 475, 1890
Eam1104I CTCTTC 3 cut(s) 768, 1023, 2049
EarI CTCTTC 3 cut(s) 768, 1023, 2049
Ecl136II GAGCTC 2 cut(s) 1210, 1503
Eco130I CCWWGG 5 cut(s) 98, 412, 1286, 1476, 1811
Eco24I GRGCYC 2 cut(s) 1212, 1505
Eco53kI GAGCTC 2 cut(s) 1210, 1503
Eco57I CTGAAG 5 cut(s) 255, 348, 849, 1319, 1472
Eco88I CYCGRG 1 cut(s) 1045
EcoICRI GAGCTC 2 cut(s) 1210, 1503
EcoO109I RGGNCCY 1 cut(s) 1808
EcoRI GAATTC 3 cut(s) 569, 599, 1751
EcoRII CCWGG 4 cut(s) 289, 630, 1179, 2047
EcoT14I CCWWGG 5 cut(s) 98, 412, 1286, 1476, 1811
EcoT22I ATGCAT 1 cut(s) 1844
EcoT38I GRGCYC 2 cut(s) 1212, 1505
ErhI CCWWGG 5 cut(s) 98, 412, 1286, 1476, 1811
Esp3I CGTCTC 1 cut(s) 269
FaqI GGGAC 2 cut(s) 1246, 1895
FauI CCCGC 1 cut(s) 1738
FbaI TGATCA 2 cut(s) 244, 676
Fnu4HI GCNGC 4 cut(s) 580, 583, 586, 1042
FokI GGATG 5 cut(s) 214, 782, 1286, 1516, 1569
FriOI GRGCYC 2 cut(s) 1212, 1505
Fsp4HI GCNGC 4 cut(s) 580, 583, 586, 1042
FspBI CTAG 4 cut(s) 99, 315, 1812, 1952
GlaI GCGC 2 cut(s) 1364, 1850
GluI GCNGC 4 cut(s) 580, 583, 586, 1042
GsuI CTGGAG 1 cut(s) 2008
HaeIII GGCC 4 cut(s) 225, 1233, 1691, 1810
HapII CCGG 4 cut(s) 359, 943, 1520, 1868
HgaI GACGC 1 cut(s) 441
HhaI GCGC 2 cut(s) 1365, 1851
Hin1I GRCGYC 1 cut(s) 452
Hin6I GCGC 2 cut(s) 1363, 1849
HinP1I GCGC 2 cut(s) 1363, 1849
HincII GTYRAC 1 cut(s) 2193
HindII GTYRAC 1 cut(s) 2193
HindIII AAGCTT 2 cut(s) 1697, 2078
HpaI GTTAAC 1 cut(s) 2193
HpaII CCGG 4 cut(s) 359, 943, 1520, 1868
HphI GGTGA 9 cut(s) 100, 200, 443, 686, 719, 760, 1328, 1606, 1882
Hpy166II GTNNAC 3 cut(s) 1414, 1771, 2193
Hpy8I GTNNAC 3 cut(s) 1414, 1771, 2193
Hpy99I CGWCG 2 cut(s) 457, 989
HpyAV CCTTC 4 cut(s) 662, 1049, 1679, 1681
HpyCH4III ACNGT 5 cut(s) 194, 661, 1197, 1761, 2132
HpyCH4IV ACGT 2 cut(s) 1827, 2116
HpyF10VI GCNNNNNNNGC 3 cut(s) 585, 647, 1848
HpyF3I CTNAG 4 cut(s) 167, 363, 381, 1513
HpySE526I ACGT 2 cut(s) 1827, 2116
Hsp92I GRCGYC 1 cut(s) 452
HspAI GCGC 2 cut(s) 1363, 1849
Ksp22I TGATCA 2 cut(s) 244, 676
KspAI GTTAAC 1 cut(s) 2193
LguI GCTCTTC 2 cut(s) 768, 2049
LmnI GCTCC 4 cut(s) 1207, 1508, 1522, 1717
Lsp1109I GCAGC 4 cut(s) 591, 594, 597, 1053
LweI GCATC 3 cut(s) 1547, 1829, 1851
MaeI CTAG 4 cut(s) 99, 315, 1812, 1952
MaeII ACGT 2 cut(s) 1827, 2116
MaeIII GTNAC 3 cut(s) 188, 748, 1352
MfeI CAATTG 1 cut(s) 1083
MflI RGATCY 5 cut(s) 757, 1330, 1480, 1665, 2091
MhlI GDGCHC 4 cut(s) 969, 1212, 1505, 1773
MlyI GAGTC 4 cut(s) 796, 980, 1057, 1268
MmeI TCCRAC 2 cut(s) 1608, 1780
Mph1103I ATGCAT 1 cut(s) 1844
MroXI GAANNNNTTC 2 cut(s) 1014, 1726
MslI CAYNNNNRTG 2 cut(s) 768, 1314
MspI CCGG 4 cut(s) 359, 943, 1520, 1868
MspR9I CCNGG 4 cut(s) 291, 632, 1181, 2049
MunI CAATTG 1 cut(s) 1083
Mva1269I GAATGC 2 cut(s) 719, 2213
MvaI CCWGG 4 cut(s) 291, 632, 1181, 2049
MvnI CGCG 2 cut(s) 1745, 1849
MwoI GCNNNNNNNGC 3 cut(s) 585, 647, 1848
NcoI CCATGG 2 cut(s) 412, 1286
NlaIV GGNNCC 3 cut(s) 7, 1482, 1713
NmeAIII GCCGAG 1 cut(s) 486
NmuCI GTSAC 2 cut(s) 188, 748
NsiI ATGCAT 1 cut(s) 1844
NspI RCATGY 4 cut(s) 1369, 2015, 2034, 2142
NspV TTCGAA 2 cut(s) 404, 1095
PacI TTAATTAA 1 cut(s) 15
PaeI GCATGC 1 cut(s) 1369
PaeR7I CTCGAG 1 cut(s) 1045
PagI TCATGA 3 cut(s) 679, 694, 826
PaqCI CACCTGC 1 cut(s) 695
PciI ACATGT 1 cut(s) 2138
PciSI GCTCTTC 2 cut(s) 768, 2049
PctI GAATGC 2 cut(s) 719, 2213
PdmI GAANNNNTTC 2 cut(s) 1014, 1726
PfoI TCCNGGA 1 cut(s) 630
PkrI GCNGC 4 cut(s) 581, 584, 587, 1043
PleI GAGTC 4 cut(s) 795, 979, 1056, 1267
PpsI GAGTC 4 cut(s) 795, 979, 1056, 1267
PscI ACATGT 1 cut(s) 2138
PshBI ATTAAT 1 cut(s) 15
Psp124BI GAGCTC 2 cut(s) 1212, 1505
Psp6I CCWGG 4 cut(s) 289, 630, 1179, 2047
PspGI CCWGG 4 cut(s) 289, 630, 1179, 2047
PspN4I GGNNCC 3 cut(s) 7, 1482, 1713
PspPI GGNCC 2 cut(s) 224, 1808
PspXI VCTCGAGB 1 cut(s) 1045
PsuI RGATCY 5 cut(s) 757, 1330, 1480, 1665, 2091
RsaI GTAC 6 cut(s) 485, 1037, 1733, 2023, 2115, 2200
RsaNI GTAC 6 cut(s) 484, 1036, 1732, 2022, 2114, 2199
RseI CAYNNNNRTG 2 cut(s) 768, 1314
SacI GAGCTC 2 cut(s) 1212, 1505
SapI GCTCTTC 2 cut(s) 768, 2049
SatI GCNGC 4 cut(s) 580, 583, 586, 1042
Sau96I GGNCC 2 cut(s) 224, 1808
SchI GAGTC 4 cut(s) 796, 980, 1057, 1268
ScrFI CCNGG 4 cut(s) 291, 632, 1181, 2049
SduI GDGCHC 4 cut(s) 969, 1212, 1505, 1773
SfaNI GCATC 3 cut(s) 1547, 1829, 1851
Sfr274I CTCGAG 1 cut(s) 1045
SfuI TTCGAA 2 cut(s) 404, 1095
SlaI CTCGAG 1 cut(s) 1045
SmiMI CAYNNNNRTG 2 cut(s) 768, 1314
SmlI CTYRAG 3 cut(s) 947, 1045, 1211
SmoI CTYRAG 3 cut(s) 947, 1045, 1211
SphI GCATGC 1 cut(s) 1369
SsiI CCGC 1 cut(s) 1745
SspI AATATT 1 cut(s) 501
SspMI CTAG 4 cut(s) 99, 315, 1812, 1952
SstI GAGCTC 2 cut(s) 1212, 1505
StyD4I CCNGG 4 cut(s) 289, 630, 1179, 2047
StyI CCWWGG 5 cut(s) 98, 412, 1286, 1476, 1811
TaaI ACNGT 5 cut(s) 194, 661, 1197, 1761, 2132
TaiI ACGT 2 cut(s) 1830, 2119
TatI WGTACW 2 cut(s) 1035, 2198
TseFI GTSAC 2 cut(s) 188, 748
TseI GCWGC 4 cut(s) 579, 582, 585, 1041
Tsp45I GTSAC 2 cut(s) 188, 748
TspGWI ACGGA 4 cut(s) 293, 767, 1704, 2137
VneI GTGCAC 1 cut(s) 1769
VspI ATTAAT 1 cut(s) 15
XapI RAATTY 9 cut(s) 476, 569, 599, 698, 952, 1014, 1373, 1546, 1751
XceI RCATGY 4 cut(s) 1369, 2015, 2034, 2142
XhoI CTCGAG 1 cut(s) 1045
XmaJI CCTAGG 2 cut(s) 98, 1811
XmnI GAANNNNTTC 2 cut(s) 1014, 1726
XspI CTAG 4 cut(s) 99, 315, 1812, 1952
Zsp2I ATGCAT 1 cut(s) 1844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.