pycom17g06700

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0002611
Physical Location & Seq
Forward (+)
4853893 .. 4856335
2443 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1452 bp
ATGGCTGCTGATCATCCATTTATGAGATGTTGGCCGATCGTGACGGCGTTGGTGTGGGCGTCGGCGGTGCAGCTGGTTGGGATTTGGTTGATTAGGGGGCCTAGTTTGTTCAATGTTTGGCCTCCTTGCACCCACTTGGAATTGCCTGCTCATGCTGTAGAATTGGCTAATAAGCTTCCAATGCCAATTGTTCTCCCACCAAAGAGGGTTTACAAAAACAATGGCTATCTCATGGTTTCTTGCAATGGAGGGCTGAATCAAATGCGAGCGGCAATCTGTGATATGGTTTCAGTTGCCAGATACTTGAATGTCACCCTTATAGTTCCTGAGCTGGATAAAAACTCCTTTTGGGCTGCGAGTGAGTTTCAGGACATTTTCGATGTTGATCATTTCATTGCATCGTTGAGAGACGAGGTTCGGATATTGAGGAAATTGCCACCAAAGCTCAAGAAAAGACCTGCATACTCGTTGCCACCAGTTAGTTGGTCTAACATGTCCTACTACCTTCAGCAGATCCTGCCTCTGGTGCAGAAGTATAAAGTAGTACATTTGAATAAAACCGATGCTCGAGTAGCTAACAATGGGCTACCTCTGGAGTTCCAGAAGCTGCGCTGCAGGGTTAATTTCCATGCTCTGAGATTCACTTCCCAGATAGAGGAAGTTGGTAGAAAGCTTGTCGGAATTTTGAGAGAAAAAGGCCCTTTTCTGGCTCTTCATCTCCGATATGAAATGGATATGTTGGCATTCTCTGGCTGCAGTCATGGGTGCAGCAATGATGAGGTAGAAGAACTCACAAGAATGAGATATGCTAATCCTTTGTGGAAGATAAAAGACATAAATTCCGAAACAAAGAGGCTAGAAGGTTTGTGCCCACTAACACCAGAGGAAACTGCTCTAGTATTGGTTGCACTCGGTATTGATCACAATGTCCAGATTTATATTGCTGCTGGAGAAATGTATGGTGGAAACAGAAGGATGGAAGGTTTGAAAGCTGCTTTTCCGAATTTGGTCAGAAAAGAGACGCTGCTGGAGCCTTCGGATTTGAGGTTCTTCCCGAACCACTCTTCCCAAATGGCAGCATTGGACTATCTAGTCTCCCTAGAGAGTGATATATTTATTCCTACATATGATGGAAACATGGCTAAAGTTGTCGAAGGCCATCGCAGATTCTTAGGTTTCAAAAAGACAGTTTTATTGGACAGAAGGCTACTGATTGGATTAATAGAACAATTCAGCACTGGCTCATTGAGTTGGGATGAGTTCTCTTACATTGTGAAGGAAGCTCACGCCGAGCGAATGGGGAGCCCGAAGAAGCGAGTTTTGATCCCAGATAAACCCAAGGAGGAAGATTACTTCTACTCAAATCCACATGAATGTTTGCAACTGTTAGATGGACCCTTGAGAACCGGATCGATCTTCGCATGCCAAAAATATGGGTTTATTGTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

484

Amino Acids

55.08

Weight (kDa)

8.75

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 76 - 390 1.4e-105 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1091
Acc36I ACCTGC 1 cut(s) 466
AccBSI CCGCTC 1 cut(s) 269
AciI CCGC 2 cut(s) 65, 269
AclWI GGATC 3 cut(s) 508, 1318, 1417
AcoI YGGCCR 1 cut(s) 32
AcsI RAATTY 3 cut(s) 681, 838, 1003
AcuI CTGAAG 1 cut(s) 491
AcyI GRCGYC 1 cut(s) 59
AfaI GTAC 1 cut(s) 546
AfiI CCNNNNNNNGG 3 cut(s) 523, 655, 706
AflIII ACRYGT 1 cut(s) 492
AgsI TTSAA 5 cut(s) 112, 307, 553, 988, 1180
AloI GAACNNNNNNTCC 4 cut(s) 1031, 1049, 1063, 1081
AluBI AGCT 9 cut(s) 73, 175, 331, 445, 575, 607, 673, 992, 1283
AluI AGCT 9 cut(s) 73, 175, 331, 445, 575, 607, 673, 992, 1283
Alw26I GTCTC 3 cut(s) 402, 1013, 1099
AlwI GGATC 3 cut(s) 508, 1318, 1417
AlwNI CAGNNNCTG 2 cut(s) 517, 607
Ama87I CYCGRG 1 cut(s) 567
AoxI GGCC 5 cut(s) 32, 98, 119, 697, 1156
ApoI RAATTY 3 cut(s) 681, 838, 1003
AseI ATTAAT 1 cut(s) 1220
AspLEI GCGC 1 cut(s) 612
AspS9I GGNCC 3 cut(s) 98, 698, 1394
AsuHPI GGTGA 1 cut(s) 304
AvaI CYCGRG 1 cut(s) 567
AvaII GGWCC 1 cut(s) 1394
BaeGI GKGCMC 1 cut(s) 872
BanII GRGCYC 1 cut(s) 1307
BbsI GAAGAC 1 cut(s) 1438
BccI CCATC 4 cut(s) 970, 1124, 1167, 1385
BceAI ACGGC 1 cut(s) 60
BclI TGATCA 3 cut(s) 10, 385, 919
BcoDI GTCTC 3 cut(s) 402, 1013, 1099
BfaI CTAG 5 cut(s) 102, 857, 896, 1091, 1100
BfmI CTRYAG 3 cut(s) 156, 613, 754
BfuAI ACCTGC 1 cut(s) 466
Bme18I GGWCC 1 cut(s) 1394
BmeT110I CYCGRG 1 cut(s) 567
BmgT120I GGNCC 3 cut(s) 98, 698, 1394
BmiI GGNNCC 4 cut(s) 99, 1032, 1304, 1396
BmsI GCATC 2 cut(s) 407, 553
BpiI GAAGAC 1 cut(s) 1438
BpmI CTGGAG 3 cut(s) 614, 969, 1049
Bpu10I CCTNAGC 1 cut(s) 327
BpuEI CTTGAG 2 cut(s) 431, 1420
Bsa29I ATCGAT 1 cut(s) 1412
BsaBI GATNNNNATC 1 cut(s) 384
BsaHI GRCGYC 1 cut(s) 59
BsaJI CCNNGG 1 cut(s) 1338
BsaWI WCCGGW 1 cut(s) 1406
Bsc4I CCNNNNNNNGG 3 cut(s) 523, 655, 706
Bse1I ACTGG 2 cut(s) 476, 1243
Bse3DI GCAATG 3 cut(s) 250, 393, 778
Bse8I GATNNNNATC 1 cut(s) 384
BseCI ATCGAT 1 cut(s) 1412
BseDI CCNNGG 1 cut(s) 1338
BseGI GGATG 3 cut(s) 13, 981, 1261
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 3 cut(s) 523, 655, 706
BseMI GCAATG 3 cut(s) 250, 393, 778
BseMII CTCAG 2 cut(s) 318, 626
BseNI ACTGG 2 cut(s) 476, 1243
BseSI GKGCMC 1 cut(s) 872
BsgI GTGCAG 3 cut(s) 89, 548, 787
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 5 cut(s) 34, 100, 121, 699, 1158
BshVI ATCGAT 1 cut(s) 1412
BsiEI CGRYCG 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 567
BsiSI CCGG 1 cut(s) 1407
BslI CCNNNNNNNGG 3 cut(s) 523, 655, 706
BsmAI GTCTC 3 cut(s) 402, 1013, 1099
BsmBI CGTCTC 2 cut(s) 402, 1013
BsmI GAATGC 1 cut(s) 743
BsnI GGCC 5 cut(s) 34, 100, 121, 699, 1158
BsoBI CYCGRG 1 cut(s) 567
Bsp1286I GDGCHC 2 cut(s) 872, 1307
Bsp143I GATC 8 cut(s) 10, 36, 385, 513, 919, 1323, 1409, 1413
BspACI CCGC 2 cut(s) 65, 269
BspANI GGCC 5 cut(s) 34, 100, 121, 699, 1158
BspCNI CTCAG 2 cut(s) 319, 627
BspDI ATCGAT 1 cut(s) 1412
BspLI GGNNCC 4 cut(s) 99, 1032, 1304, 1396
BspMAI CTGCAG 2 cut(s) 617, 758
BspMI ACCTGC 1 cut(s) 466
BspPI GGATC 3 cut(s) 508, 1318, 1417
BspQI GCTCTTC 1 cut(s) 717
BsrBI CCGCTC 1 cut(s) 269
BsrDI GCAATG 3 cut(s) 250, 393, 778
BsrI ACTGG 2 cut(s) 476, 1243
BssECI CCNNGG 1 cut(s) 1338
BssMI GATC 8 cut(s) 10, 36, 385, 513, 919, 1323, 1409, 1413
BssNI GRCGYC 1 cut(s) 59
BssT1I CCWWGG 1 cut(s) 1338
Bst4CI ACNGT 2 cut(s) 1189, 1386
Bst6I CTCTTC 2 cut(s) 717, 1069
BstACI GRCGYC 1 cut(s) 59
BstAPI GCANNNNNTGC 1 cut(s) 517
BstC8I GCNNGC 3 cut(s) 147, 267, 1423
BstDEI CTNAG 3 cut(s) 327, 635, 1171
BstF5I GGATG 3 cut(s) 13, 981, 1261
BstHHI GCGC 1 cut(s) 612
BstKTI GATC 8 cut(s) 13, 39, 388, 516, 922, 1326, 1412, 1416
BstMAI GTCTC 3 cut(s) 402, 1013, 1099
BstMBI GATC 8 cut(s) 10, 36, 385, 513, 919, 1323, 1409, 1413
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 6 cut(s) 181, 442, 517, 526, 572, 1030
BstNSI RCATGY 2 cut(s) 496, 1425
BstSFI CTRYAG 3 cut(s) 156, 613, 754
BstSLI GKGCMC 1 cut(s) 872
BstV2I GAAGAC 1 cut(s) 1438
BstX2I RGATCY 1 cut(s) 513
BstXI CCANNNNNNTGG 2 cut(s) 483, 1433
BstYI RGATCY 1 cut(s) 513
Bsu15I ATCGAT 1 cut(s) 1412
BsuRI GGCC 5 cut(s) 34, 100, 121, 699, 1158
BsuTUI ATCGAT 1 cut(s) 1412
BtgZI GCGATG 1 cut(s) 1145
BtsCI GGATG 3 cut(s) 13, 981, 1261
BtsIMutI CAGTG 1 cut(s) 1236
BveI ACCTGC 1 cut(s) 466
Cac8I GCNNGC 3 cut(s) 147, 267, 1423
CaiI CAGNNNCTG 2 cut(s) 517, 607
CfoI GCGC 1 cut(s) 612
Cfr13I GGNCC 3 cut(s) 98, 698, 1394
ClaI ATCGAT 1 cut(s) 1412
CseI GACGC 2 cut(s) 48, 1030
Csp6I GTAC 1 cut(s) 545
CviAII CATG 8 cut(s) 152, 232, 493, 629, 761, 1138, 1370, 1422
CviQI GTAC 1 cut(s) 545
DdeI CTNAG 3 cut(s) 327, 635, 1171
DpnI GATC 8 cut(s) 12, 38, 387, 515, 921, 1325, 1411, 1415
DpnII GATC 8 cut(s) 10, 36, 385, 513, 919, 1323, 1409, 1413
DrdI GACNNNNNNGTC 1 cut(s) 1091
DseDI GACNNNNNNGTC 1 cut(s) 1091
EaeI YGGCCR 1 cut(s) 32
Eam1104I CTCTTC 2 cut(s) 717, 1069
EarI CTCTTC 2 cut(s) 717, 1069
Eco130I CCWWGG 1 cut(s) 1338
Eco24I GRGCYC 1 cut(s) 1307
Eco47I GGWCC 1 cut(s) 1394
Eco57I CTGAAG 1 cut(s) 491
Eco88I CYCGRG 1 cut(s) 567
EcoO109I RGGNCCY 2 cut(s) 98, 698
EcoT14I CCWWGG 1 cut(s) 1338
EcoT38I GRGCYC 1 cut(s) 1307
ErhI CCWWGG 1 cut(s) 1338
Esp3I CGTCTC 2 cut(s) 402, 1013
FaeI CATG 8 cut(s) 155, 235, 496, 632, 764, 1141, 1373, 1425
FatI CATG 8 cut(s) 151, 231, 492, 628, 760, 1137, 1369, 1421
FauNDI CATATG 1 cut(s) 1126
FbaI TGATCA 3 cut(s) 10, 385, 919
FokI GGATG 2 cut(s) 988, 1268
FriOI GRGCYC 1 cut(s) 1307
FspBI CTAG 5 cut(s) 102, 857, 896, 1091, 1100
GlaI GCGC 1 cut(s) 611
GsuI CTGGAG 3 cut(s) 614, 969, 1049
HaeIII GGCC 5 cut(s) 34, 100, 121, 699, 1158
HapII CCGG 1 cut(s) 1407
HgaI GACGC 2 cut(s) 48, 1030
HhaI GCGC 1 cut(s) 612
Hin1I GRCGYC 1 cut(s) 59
Hin1II CATG 8 cut(s) 155, 235, 496, 632, 764, 1141, 1373, 1425
Hin6I GCGC 1 cut(s) 610
HinP1I GCGC 1 cut(s) 610
HindIII AAGCTT 2 cut(s) 173, 671
HinfI GANTC 3 cut(s) 256, 639, 1167
HpaII CCGG 1 cut(s) 1407
HphI GGTGA 1 cut(s) 304
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 8 cut(s) 420, 636, 680, 722, 844, 1002, 1013, 1039
Hpy188III TCNNGA 8 cut(s) 40, 326, 368, 448, 593, 601, 931, 1054
Hpy8I GTNNAC 1 cut(s) 211
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 8 cut(s) 515, 854, 966, 974, 1044, 1148, 1197, 1270
HpyCH4III ACNGT 2 cut(s) 1189, 1386
HpyF10VI GCNNNNNNNGC 6 cut(s) 181, 442, 517, 526, 572, 1030
HpyF3I CTNAG 3 cut(s) 327, 635, 1171
Hsp92I GRCGYC 1 cut(s) 59
Hsp92II CATG 8 cut(s) 155, 235, 496, 632, 764, 1141, 1373, 1425
HspAI GCGC 1 cut(s) 610
Ksp22I TGATCA 3 cut(s) 10, 385, 919
Kzo9I GATC 8 cut(s) 10, 36, 385, 513, 919, 1323, 1409, 1413
LguI GCTCTTC 1 cut(s) 717
LmnI GCTCC 2 cut(s) 1030, 1302
LweI GCATC 2 cut(s) 407, 553
MaeI CTAG 5 cut(s) 102, 857, 896, 1091, 1100
MaeIII GTNAC 2 cut(s) 40, 310
MalI GATC 8 cut(s) 12, 38, 387, 515, 921, 1325, 1411, 1415
MbiI CCGCTC 1 cut(s) 269
MboI GATC 8 cut(s) 10, 36, 385, 513, 919, 1323, 1409, 1413
MboII GAAGA 9 cut(s) 704, 797, 835, 1042, 1056, 1321, 1358, 1408, 1438
MfeI CAATTG 1 cut(s) 186
MflI RGATCY 1 cut(s) 513
MhlI GDGCHC 2 cut(s) 872, 1307
MluCI AATT 9 cut(s) 140, 161, 186, 431, 622, 681, 838, 1003, 1229
MmeI TCCRAC 1 cut(s) 658
MseI TTAA 2 cut(s) 621, 1220
MslI CAYNNNNRTG 2 cut(s) 797, 1372
MspA1I CMGCKG 1 cut(s) 73
MspI CCGG 1 cut(s) 1407
MunI CAATTG 1 cut(s) 186
Mva1269I GAATGC 1 cut(s) 743
MwoI GCNNNNNNNGC 6 cut(s) 181, 442, 517, 526, 572, 1030
NdeI CATATG 1 cut(s) 1126
NdeII GATC 8 cut(s) 10, 36, 385, 513, 919, 1323, 1409, 1413
NlaIII CATG 8 cut(s) 155, 235, 496, 632, 764, 1141, 1373, 1425
NlaIV GGNNCC 4 cut(s) 99, 1032, 1304, 1396
NmeAIII GCCGAG 1 cut(s) 1315
NmuCI GTSAC 2 cut(s) 40, 310
NspI RCATGY 2 cut(s) 496, 1425
PaeI GCATGC 1 cut(s) 1425
PaeR7I CTCGAG 1 cut(s) 567
PciI ACATGT 1 cut(s) 492
PciSI GCTCTTC 1 cut(s) 717
PctI GAATGC 1 cut(s) 743
PfeI GAWTC 3 cut(s) 256, 639, 1167
Ple19I CGATCG 1 cut(s) 39
PscI ACATGT 1 cut(s) 492
PshBI ATTAAT 1 cut(s) 1220
PspN4I GGNNCC 4 cut(s) 99, 1032, 1304, 1396
PspPI GGNCC 3 cut(s) 98, 698, 1394
PspXI VCTCGAGB 1 cut(s) 567
PstI CTGCAG 2 cut(s) 617, 758
PstNI CAGNNNCTG 2 cut(s) 517, 607
PsuI RGATCY 1 cut(s) 513
PvuI CGATCG 1 cut(s) 39
PvuII CAGCTG 1 cut(s) 73
RsaI GTAC 1 cut(s) 546
RsaNI GTAC 1 cut(s) 545
RseI CAYNNNNRTG 2 cut(s) 797, 1372
SapI GCTCTTC 1 cut(s) 717
SaqAI TTAA 2 cut(s) 621, 1220
Sau3AI GATC 8 cut(s) 10, 36, 385, 513, 919, 1323, 1409, 1413
Sau96I GGNCC 3 cut(s) 98, 698, 1394
SduI GDGCHC 2 cut(s) 872, 1307
SfaNI GCATC 2 cut(s) 407, 553
SfcI CTRYAG 3 cut(s) 156, 613, 754
Sfr274I CTCGAG 1 cut(s) 567
SinI GGWCC 1 cut(s) 1394
SlaI CTCGAG 1 cut(s) 567
SmiMI CAYNNNNRTG 2 cut(s) 797, 1372
SmlI CTYRAG 3 cut(s) 446, 567, 1399
SmoI CTYRAG 3 cut(s) 446, 567, 1399
SphI GCATGC 1 cut(s) 1425
Sse9I AATT 9 cut(s) 140, 161, 186, 431, 622, 681, 838, 1003, 1229
SsiI CCGC 2 cut(s) 65, 269
SspMI CTAG 5 cut(s) 102, 857, 896, 1091, 1100
StyI CCWWGG 1 cut(s) 1338
TaaI ACNGT 2 cut(s) 1189, 1386
TaqI TCGA 4 cut(s) 378, 568, 1152, 1412
TasI AATT 9 cut(s) 140, 161, 186, 431, 622, 681, 838, 1003, 1229
TatI WGTACW 1 cut(s) 544
TauI GCSGC 1 cut(s) 272
TfiI GAWTC 3 cut(s) 256, 639, 1167
Tru1I TTAA 2 cut(s) 621, 1220
Tru9I TTAA 2 cut(s) 621, 1220
TscAI CASTG 1 cut(s) 1243
TseFI GTSAC 2 cut(s) 40, 310
Tsp45I GTSAC 2 cut(s) 40, 310
TspDTI ATGAA 4 cut(s) 382, 704, 741, 1386
TspRI CASTG 1 cut(s) 1243
VpaK11BI GGWCC 1 cut(s) 1394
VspI ATTAAT 1 cut(s) 1220
XapI RAATTY 3 cut(s) 681, 838, 1003
XceI RCATGY 2 cut(s) 496, 1425
XcmI CCANNNNNNNNNTGG 1 cut(s) 480
XhoI CTCGAG 1 cut(s) 567
XspI CTAG 5 cut(s) 102, 857, 896, 1091, 1100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.