RchiOBHm_Chr7g0180481

Ent-kaurenoic acid oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0004857
Physical Location & Seq
Forward (+)
2378701 .. 2382686
3986 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1452 bp
ATGGAGATCTCTTTGGGGGTATGGGTGGCCTTGGTTCTGGGAGGTTTGCCATTTTGGGGGTTGTTGTTGTGGTGGTGGAATGAGTTCTGGTATGTGCTTCCTCTTAAGTTGCGCGGGTCTTCGACTGGCGCCAAATTGCCTCCAGGTCACCTGGGTTTTCCATTTCTTGGTGAAATGCTCAGTTTCTTGTGGTATTTCAAAATTGTTTGCCATCCTGATGAGTTTATCAATGCCAAGAGAGCAAAGTACGGTGATGGAGTAGGAATGTACAGAACACACATGTTCGGAAAGCCATCCATCATAACCTGCTTACCGGAGATCAACAAATTAGTATTCCAATCAGAGGAGAACTTTCCCCTAAATTGGCCTAATGTTGATATTTTAGGTTCTTGTTCTTTGGTAGCAGTTCATGGAAGCTCCCATGCAAGGCTTAGAAGCTACGTCGTGAATGTCATTAACAAACCTGATGCTCTGCGCCGCATTGCCGGACTAGTTCAGCCACGTATAGTGGCTGCATTGGAGTCATGGGCACTCAAAGGTAGAATAAAAGCATATGATGAATCAAAGAAGGTAACATTTGAAAATATTGGAAAATTATTCGCAAGTTTGGAGCCGGGGCCTCTCCTAGATACCATTGATAAATTGTTTGAGGGAGTGGTCAAAGGAATTAGAGCTCAACCATTCAATGTTCCTGGATCTGCTTATCACCATGCTCTTCAGTGCAGGAGGAAGCTTGAGGCGATTTTCAGGGTGGAGCTAGAGAAGAAAAAATTCCGAAAAGAAGCAGTGACGACTGATCTGATGGATGGGCTTATGCAAATCACGGATGATGAGGGTGATAAATTAAGTGACAAAGAGGTTATAGATAACATTGTGAGCCTTGTTATTGCTGGATATGCATCCACTGCCCTTTCAGCAATGTGGGCTATCTTCTATCTTGCCAAATACCCCAATGTTTTTAAAAAGCTTCGGGAGGAGAATATGATTGTAAGTCAGAACAAGAACGGGGAATTCATTACAAGTGACGATGTTTCGAAAATGAAATACACGAACAAGGTGGTGGAAGAAATTATAAGACTGGCAAACATTGCAGCTTTTGTTTTCCGGTCAGTTACCAAGGAAATCGAGTATAAAGGCTACATAATACCAAAGAATTGGAATATGCTTGTTTGGGTACGTTATCTCCATACAAATTCAGAGCACTTTGATGATCCTATGTGCTTTAAACCAGATAGATGGAATAATCCGGCTAAACCTGGAACATACCAAGTTTTCGGAGGTGGATCAAGAATATGTGCAGGAAATATGCTTGTTAGACTACAACTCGCAATTTTCTTACATCATTTGTCTGTTGGATACAAGTGGAAATTACTCAATCCCAATGCCTGTATGAATTATCTGCCACATCCAAGACCGGCTGATAATGTCGAGGTCACATTCAGCAAAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00904 ko01100 ko01110 map00904 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

483

Amino Acids

54.97

Weight (kDa)

9.16

Isoelectric Point (pI)

33.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 47 - 457 2.2e-49 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1073
Acc36I ACCTGC 1 cut(s) 314
AccB1I GGYRCC 1 cut(s) 128
AccB7I CCANNNNNTGG 1 cut(s) 167
AccII CGCG 1 cut(s) 114
AciI CCGC 2 cut(s) 114, 478
AclWI GGATC 3 cut(s) 703, 1205, 1291
AcsI RAATTY 4 cut(s) 770, 1010, 1192, 1446
AcuI CTGAAG 1 cut(s) 701
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 3 cut(s) 248, 269, 1176
AfiI CCNNNNNNNGG 5 cut(s) 56, 167, 343, 363, 426
AflII CTTAAG 1 cut(s) 104
AflIII ACRYGT 1 cut(s) 279
AgsI TTSAA 3 cut(s) 199, 581, 685
AhlI ACTAGT 1 cut(s) 490
AjnI CCWGG 4 cut(s) 142, 150, 691, 1255
AluBI AGCT 7 cut(s) 417, 438, 674, 733, 757, 967, 1094
AluI AGCT 7 cut(s) 417, 438, 674, 733, 757, 967, 1094
Alw21I GWGCWC 2 cut(s) 676, 1203
AlwI GGATC 3 cut(s) 703, 1205, 1291
AoxI GGCC 3 cut(s) 27, 365, 617
ApeKI GCWGC 2 cut(s) 512, 1091
ApoI RAATTY 4 cut(s) 770, 1010, 1192, 1446
Asp700I GAANNNNTTC 2 cut(s) 83, 770
AspLEI GCGC 3 cut(s) 114, 131, 477
AspS9I GGNCC 1 cut(s) 617
AsuC2I CCSGG 1 cut(s) 615
AsuHPI GGTGA 5 cut(s) 140, 182, 263, 698, 848
AsuII TTCGAA 1 cut(s) 1034
BaeGI GKGCMC 1 cut(s) 532
BanI GGYRCC 1 cut(s) 128
BanII GRGCYC 1 cut(s) 676
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 2 cut(s) 676, 1203
BbvI GCAGC 2 cut(s) 499, 1103
BccI CCATC 7 cut(s) 219, 248, 301, 305, 796, 800, 1230
BciT130I CCWGG 4 cut(s) 144, 152, 693, 1257
BciVI GTATCC 1 cut(s) 1349
BcnI CCSGG 1 cut(s) 615
BcuI ACTAGT 1 cut(s) 490
BfaI CTAG 3 cut(s) 491, 626, 758
BfoI RGCGCY 1 cut(s) 132
BfrI CTTAAG 1 cut(s) 104
BfuAI ACCTGC 1 cut(s) 314
BfuI GTATCC 1 cut(s) 1349
BglII AGATCT 1 cut(s) 6
BisI GCNGC 3 cut(s) 478, 513, 1092
BlsI GCNGC 3 cut(s) 479, 514, 1093
Bme1390I CCNGG 5 cut(s) 144, 152, 615, 693, 1257
BmgT120I GGNCC 1 cut(s) 617
BmiI GGNNCC 3 cut(s) 130, 612, 618
BmrFI CCNGG 5 cut(s) 144, 152, 615, 693, 1257
BmsI GCATC 2 cut(s) 457, 908
BpiI GAAGAC 1 cut(s) 111
BpmI CTGGAG 1 cut(s) 126
Bpu14I TTCGAA 1 cut(s) 1034
BpuEI CTTGAG 1 cut(s) 755
BpuMI CCSGG 1 cut(s) 615
BsaAI YACGTR 1 cut(s) 503
BsaBI GATNNNNATC 1 cut(s) 898
BsaHI GRCGYC 1 cut(s) 129
BsaJI CCNNGG 4 cut(s) 30, 151, 614, 1116
BsaWI WCCGGW 2 cut(s) 313, 1104
BsaXI ACNNNNNCTCC 2 cut(s) 1167, 1197
Bsc4I CCNNNNNNNGG 5 cut(s) 56, 167, 343, 363, 426
Bse118I RCCGGY 1 cut(s) 1414
Bse1I ACTGG 2 cut(s) 130, 1083
Bse3DI GCAATG 3 cut(s) 480, 924, 1086
Bse8I GATNNNNATC 1 cut(s) 898
BseBI CCWGG 4 cut(s) 144, 152, 693, 1257
BseDI CCNNGG 4 cut(s) 30, 151, 614, 1116
BseGI GGATG 6 cut(s) 211, 293, 811, 832, 899, 1405
BseJI GATNNNNATC 1 cut(s) 898
BseLI CCNNNNNNNGG 5 cut(s) 56, 167, 343, 363, 426
BseMI GCAATG 3 cut(s) 480, 924, 1086
BseMII CTCAG 1 cut(s) 193
BseNI ACTGG 2 cut(s) 130, 1083
BseRI GAGGAG 2 cut(s) 359, 989
BseSI GKGCMC 1 cut(s) 532
BseXI GCAGC 2 cut(s) 499, 1103
BsgI GTGCAG 2 cut(s) 742, 1317
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 3 cut(s) 29, 367, 619
BshNI GGYRCC 1 cut(s) 128
BsiHKAI GWGCWC 2 cut(s) 676, 1203
BsiSI CCGG 6 cut(s) 314, 486, 614, 1105, 1247, 1415
BslI CCNNNNNNNGG 5 cut(s) 56, 167, 343, 363, 426
BsnI GGCC 3 cut(s) 29, 367, 619
Bsp119I TTCGAA 1 cut(s) 1034
Bsp1286I GDGCHC 3 cut(s) 532, 676, 1203
Bsp1407I TGTACA 1 cut(s) 267
Bsp143I GATC 6 cut(s) 6, 318, 695, 796, 1210, 1283
BspACI CCGC 2 cut(s) 114, 478
BspANI GGCC 3 cut(s) 29, 367, 619
BspCNI CTCAG 1 cut(s) 192
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 3 cut(s) 130, 612, 618
BspMI ACCTGC 1 cut(s) 314
BspPI GGATC 3 cut(s) 703, 1205, 1291
BspQI GCTCTTC 1 cut(s) 720
BspT104I TTCGAA 1 cut(s) 1034
BspT107I GGYRCC 1 cut(s) 128
BspTI CTTAAG 1 cut(s) 104
BsrDI GCAATG 3 cut(s) 480, 924, 1086
BsrFI RCCGGY 1 cut(s) 1414
BsrGI TGTACA 1 cut(s) 267
BsrI ACTGG 2 cut(s) 130, 1083
BssAI RCCGGY 1 cut(s) 1414
BssECI CCNNGG 4 cut(s) 30, 151, 614, 1116
BssMI GATC 6 cut(s) 6, 318, 695, 796, 1210, 1283
BssNI GRCGYC 1 cut(s) 129
BssT1I CCWWGG 2 cut(s) 30, 1116
Bst2UI CCWGG 4 cut(s) 144, 152, 693, 1257
Bst4CI ACNGT 1 cut(s) 251
Bst6I CTCTTC 1 cut(s) 720
BstACI GRCGYC 1 cut(s) 129
BstAFI CTTAAG 1 cut(s) 104
BstAPI GCANNNNNTGC 2 cut(s) 905, 1088
BstAUI TGTACA 1 cut(s) 267
BstBAI YACGTR 1 cut(s) 503
BstBI TTCGAA 1 cut(s) 1034
BstDEI CTNAG 2 cut(s) 179, 431
BstEII GGTNACC 1 cut(s) 146
BstF5I GGATG 6 cut(s) 211, 293, 811, 832, 899, 1405
BstFNI CGCG 1 cut(s) 114
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 3 cut(s) 114, 131, 477
BstKTI GATC 6 cut(s) 9, 321, 698, 799, 1213, 1286
BstMBI GATC 6 cut(s) 6, 318, 695, 796, 1210, 1283
BstMWI GCNNNNNNNGC 6 cut(s) 239, 896, 905, 914, 923, 1088
BstNI CCWGG 4 cut(s) 144, 152, 693, 1257
BstNSI RCATGY 1 cut(s) 283
BstPI GGTNACC 1 cut(s) 146
BstSCI CCNGG 5 cut(s) 142, 150, 613, 691, 1255
BstSLI GKGCMC 1 cut(s) 532
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 2 cut(s) 499, 1103
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 2 cut(s) 6, 695
BstXI CCANNNNNNTGG 2 cut(s) 1155, 1236
BstYI RGATCY 2 cut(s) 6, 695
BsuI GTATCC 1 cut(s) 1349
BsuRI GGCC 3 cut(s) 29, 367, 619
BtsCI GGATG 6 cut(s) 211, 293, 811, 832, 899, 1405
BtsI GCAGTG 2 cut(s) 792, 903
BtsIMutI CAGTG 3 cut(s) 725, 792, 903
BveI ACCTGC 1 cut(s) 314
CfoI GCGC 3 cut(s) 114, 131, 477
Cfr10I RCCGGY 1 cut(s) 1414
Cfr13I GGNCC 1 cut(s) 617
Csp6I GTAC 3 cut(s) 247, 268, 1175
CviAII CATG 5 cut(s) 280, 410, 422, 525, 710
CviQI GTAC 3 cut(s) 247, 268, 1175
DdeI CTNAG 2 cut(s) 179, 431
DinI GGCGCC 1 cut(s) 130
DpnI GATC 6 cut(s) 8, 320, 697, 798, 1212, 1285
DpnII GATC 6 cut(s) 6, 318, 695, 796, 1210, 1283
DraI TTTAAA 2 cut(s) 961, 1225
Eam1104I CTCTTC 1 cut(s) 720
EarI CTCTTC 1 cut(s) 720
Ecl136II GAGCTC 1 cut(s) 674
Eco130I CCWWGG 2 cut(s) 30, 1116
Eco24I GRGCYC 1 cut(s) 676
Eco53kI GAGCTC 1 cut(s) 674
Eco57I CTGAAG 1 cut(s) 701
Eco91I GGTNACC 1 cut(s) 146
EcoICRI GAGCTC 1 cut(s) 674
EcoO109I RGGNCCY 1 cut(s) 617
EcoO65I GGTNACC 1 cut(s) 146
EcoRI GAATTC 1 cut(s) 1010
EcoRII CCWGG 4 cut(s) 142, 150, 691, 1255
EcoT14I CCWWGG 2 cut(s) 30, 1116
EcoT22I ATGCAT 1 cut(s) 901
EcoT38I GRGCYC 1 cut(s) 676
EgeI GGCGCC 1 cut(s) 130
EheI GGCGCC 1 cut(s) 130
ErhI CCWWGG 2 cut(s) 30, 1116
FaeI CATG 5 cut(s) 283, 413, 425, 528, 713
FatI CATG 5 cut(s) 279, 409, 421, 524, 709
FauI CCCGC 1 cut(s) 107
FauNDI CATATG 1 cut(s) 553
Fnu4HI GCNGC 3 cut(s) 478, 513, 1092
FokI GGATG 6 cut(s) 198, 280, 818, 839, 886, 1392
FriOI GRGCYC 1 cut(s) 676
Fsp4HI GCNGC 3 cut(s) 478, 513, 1092
FspBI CTAG 3 cut(s) 491, 626, 758
GlaI GCGC 3 cut(s) 113, 130, 476
GluI GCNGC 3 cut(s) 478, 513, 1092
GsuI CTGGAG 1 cut(s) 126
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 3 cut(s) 29, 367, 619
HapII CCGG 6 cut(s) 314, 486, 614, 1105, 1247, 1415
HhaI GCGC 3 cut(s) 114, 131, 477
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 5 cut(s) 283, 413, 425, 528, 713
Hin6I GCGC 3 cut(s) 112, 129, 475
HinP1I GCGC 3 cut(s) 112, 129, 475
HindIII AAGCTT 2 cut(s) 731, 965
HinfI GANTC 2 cut(s) 521, 560
HpaII CCGG 6 cut(s) 314, 486, 614, 1105, 1247, 1415
HphI GGTGA 5 cut(s) 140, 182, 263, 698, 848
Hpy188I TCNGA 7 cut(s) 287, 343, 776, 801, 996, 1198, 1277
Hpy188III TCNNGA 4 cut(s) 215, 445, 971, 1287
Hpy99I CGWCG 1 cut(s) 446
HpyAV CCTTC 1 cut(s) 562
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4IV ACGT 3 cut(s) 441, 502, 1177
HpyCH4V TGCA 7 cut(s) 425, 515, 723, 817, 899, 1091, 1298
HpyF10VI GCNNNNNNNGC 6 cut(s) 239, 896, 905, 914, 923, 1088
HpyF3I CTNAG 2 cut(s) 179, 431
HpySE526I ACGT 3 cut(s) 441, 502, 1177
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 5 cut(s) 283, 413, 425, 528, 713
HspAI GCGC 3 cut(s) 112, 129, 475
KasI GGCGCC 1 cut(s) 128
Kzo9I GATC 6 cut(s) 6, 318, 695, 796, 1210, 1283
LguI GCTCTTC 1 cut(s) 720
LmnI GCTCC 3 cut(s) 422, 610, 754
Lsp1109I GCAGC 2 cut(s) 499, 1103
LweI GCATC 2 cut(s) 457, 908
MaeI CTAG 3 cut(s) 491, 626, 758
MaeII ACGT 3 cut(s) 441, 502, 1177
MaeIII GTNAC 7 cut(s) 146, 571, 787, 848, 1022, 1111, 1432
MalI GATC 6 cut(s) 8, 320, 697, 798, 1212, 1285
MboI GATC 6 cut(s) 6, 318, 695, 796, 1210, 1283
MboII GAAGA 5 cut(s) 111, 707, 775, 922, 1076
MflI RGATCY 2 cut(s) 6, 695
MhlI GDGCHC 3 cut(s) 532, 676, 1203
Mly113I GGCGCC 1 cut(s) 129
MlyI GAGTC 1 cut(s) 530
MmeI TCCRAC 1 cut(s) 1333
Mph1103I ATGCAT 1 cut(s) 901
MroXI GAANNNNTTC 2 cut(s) 83, 770
MseI TTAA 5 cut(s) 105, 456, 845, 960, 1224
MslI CAYNNNNRTG 2 cut(s) 216, 1206
MspCI CTTAAG 1 cut(s) 104
MspI CCGG 6 cut(s) 314, 486, 614, 1105, 1247, 1415
MspR9I CCNGG 5 cut(s) 144, 152, 615, 693, 1257
MvaI CCWGG 4 cut(s) 144, 152, 693, 1257
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 6 cut(s) 239, 896, 905, 914, 923, 1088
NarI GGCGCC 1 cut(s) 129
NciI CCSGG 1 cut(s) 615
NdeI CATATG 1 cut(s) 553
NdeII GATC 6 cut(s) 6, 318, 695, 796, 1210, 1283
NlaIII CATG 5 cut(s) 283, 413, 425, 528, 713
NlaIV GGNNCC 3 cut(s) 130, 612, 618
NmuCI GTSAC 5 cut(s) 146, 787, 848, 1022, 1432
NsiI ATGCAT 1 cut(s) 901
NspI RCATGY 1 cut(s) 283
NspV TTCGAA 1 cut(s) 1034
PciI ACATGT 1 cut(s) 279
PciSI GCTCTTC 1 cut(s) 720
PdmI GAANNNNTTC 2 cut(s) 83, 770
PfeI GAWTC 1 cut(s) 560
PflMI CCANNNNNTGG 1 cut(s) 167
PfoI TCCNGGA 1 cut(s) 691
PkrI GCNGC 3 cut(s) 479, 514, 1093
PleI GAGTC 1 cut(s) 529
PluTI GGCGCC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 529
Ppu21I YACGTR 1 cut(s) 503
PscI ACATGT 1 cut(s) 279
PsiI TTATAA 1 cut(s) 1073
Psp124BI GAGCTC 1 cut(s) 676
Psp6I CCWGG 4 cut(s) 142, 150, 691, 1255
PspEI GGTNACC 1 cut(s) 146
PspGI CCWGG 4 cut(s) 142, 150, 691, 1255
PspN4I GGNNCC 3 cut(s) 130, 612, 618
PspPI GGNCC 1 cut(s) 617
PsuI RGATCY 2 cut(s) 6, 695
RsaI GTAC 3 cut(s) 248, 269, 1176
RsaNI GTAC 3 cut(s) 247, 268, 1175
RseI CAYNNNNRTG 2 cut(s) 216, 1206
SacI GAGCTC 1 cut(s) 676
SapI GCTCTTC 1 cut(s) 720
SaqAI TTAA 5 cut(s) 105, 456, 845, 960, 1224
SatI GCNGC 3 cut(s) 478, 513, 1092
Sau3AI GATC 6 cut(s) 6, 318, 695, 796, 1210, 1283
Sau96I GGNCC 1 cut(s) 617
SchI GAGTC 1 cut(s) 530
ScrFI CCNGG 5 cut(s) 144, 152, 615, 693, 1257
SduI GDGCHC 3 cut(s) 532, 676, 1203
SfaNI GCATC 2 cut(s) 457, 908
SfoI GGCGCC 1 cut(s) 130
SfuI TTCGAA 1 cut(s) 1034
SmiMI CAYNNNNRTG 2 cut(s) 216, 1206
SmlI CTYRAG 2 cut(s) 104, 734
SmoI CTYRAG 2 cut(s) 104, 734
SpeI ACTAGT 1 cut(s) 490
SsiI CCGC 2 cut(s) 114, 478
SspDI GGCGCC 1 cut(s) 128
SspI AATATT 1 cut(s) 586
SspMI CTAG 3 cut(s) 491, 626, 758
SstI GAGCTC 1 cut(s) 676
StyD4I CCNGG 5 cut(s) 142, 150, 613, 691, 1255
StyI CCWWGG 2 cut(s) 30, 1116
TaaI ACNGT 1 cut(s) 251
TaiI ACGT 3 cut(s) 444, 505, 1180
TaqI TCGA 4 cut(s) 122, 1034, 1125, 1428
TatI WGTACW 1 cut(s) 267
TauI GCSGC 1 cut(s) 480
TfiI GAWTC 1 cut(s) 560
Tru1I TTAA 5 cut(s) 105, 456, 845, 960, 1224
Tru9I TTAA 5 cut(s) 105, 456, 845, 960, 1224
TscAI CASTG 3 cut(s) 725, 792, 910
TseFI GTSAC 5 cut(s) 146, 787, 848, 1022, 1432
TseI GCWGC 2 cut(s) 512, 1091
Tsp45I GTSAC 5 cut(s) 146, 787, 848, 1022, 1432
TspDTI ATGAA 5 cut(s) 398, 573, 1003, 1055, 1406
TspGWI ACGGA 1 cut(s) 839
TspRI CASTG 3 cut(s) 725, 792, 910
Van91I CCANNNNNTGG 1 cut(s) 167
Vha464I CTTAAG 1 cut(s) 104
XapI RAATTY 4 cut(s) 770, 1010, 1192, 1446
XceI RCATGY 1 cut(s) 283
XmnI GAANNNNTTC 2 cut(s) 83, 770
XspI CTAG 3 cut(s) 491, 626, 758
Zsp2I ATGCAT 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.