RchiOBHm_Chr4g0385541

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0003902
Physical Location & Seq
Forward (+)
1032256 .. 1037447
5192 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 687 bp
ATGAGGTCCAAGAAAACTCCGGTTGGGTTTCGGGTCTTGGGGCAGCGCTCACTTTCTTCATCATTTTCTTCCCGCAGTAAAATCCCTGTCAAGGTTGCAGAAGATGATGCGGATAAGGGCTCGCATGGGTCGCTCTCGGACATTCCAGAACAGAGGCTGAAGCACAATTCTGGAACACCCAAGACAGTAAAGGTGAAGTCGCGGCCATTCTTGTCGTTATTGGGTCTGAAAGATTTAAGTGGGTCCACTGATGACCAAATTGAGGCCAAGAAAGGAGGGGAAGCAGAGAAGAGTTCTGTAGCATTGGCATTTGAAAAATTTAAGCACACTGATATAGGAAAAGGGCAATCTATAGTTTCACAAAGTGCTGGTGAAGCACCATGTCCTGACGGAGATGATTTACCAGAAACAAGAAAAAGGAGACACCCTTGTAAAGATGGGGATGACAAACACACTGCGAGAAAGTATTTTGCAGTTCTTGGAGGTGATCCAAAACCCAAGCTAAAAAAGAGAAGGGCTGATAGTCTCATAAGCCAGGAGAAACCAGGACATGTTTATGATCACCATGAAAATGGTTGTGGCTGGTGGGACTGCAACATGGAGGGTATTGACAATGAAGAAGTTGGAGTCAATGAGGTGTGGGAAGGAGTGGGCTCAACCACATTGGGAGGAATAGAGTGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

228

Amino Acids

24.92

Weight (kDa)

8.26

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 202
AciI CCGC 3 cut(s) 73, 110, 202
AclWI GGATC 1 cut(s) 482
AcoI YGGCCR 1 cut(s) 203
AcsI RAATTY 1 cut(s) 317
AcuI CTGAAG 1 cut(s) 179
AdeI CACNNNGTG 1 cut(s) 365
AfeI AGCGCT 1 cut(s) 47
AfiI CCNNNNNNNGG 2 cut(s) 91, 262
AflIII ACRYGT 1 cut(s) 550
AgsI TTSAA 1 cut(s) 314
AjnI CCWGG 2 cut(s) 534, 544
AluBI AGCT 1 cut(s) 502
AluI AGCT 1 cut(s) 502
Alw26I GTCTC 2 cut(s) 415, 530
AlwI GGATC 1 cut(s) 482
AlwNI CAGNNNCTG 1 cut(s) 157
Aor51HI AGCGCT 1 cut(s) 47
AoxI GGCC 2 cut(s) 203, 264
ApeKI GCWGC 1 cut(s) 43
ApoI RAATTY 1 cut(s) 317
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 2 cut(s) 6, 243
AsuHPI GGTGA 4 cut(s) 205, 383, 497, 554
AvaII GGWCC 2 cut(s) 6, 243
BanII GRGCYC 2 cut(s) 122, 656
BbvI GCAGC 1 cut(s) 55
BccI CCATC 1 cut(s) 431
BciT130I CCWGG 2 cut(s) 536, 546
BclI TGATCA 1 cut(s) 559
BcoDI GTCTC 2 cut(s) 415, 530
BfmI CTRYAG 2 cut(s) 297, 351
BfoI RGCGCY 1 cut(s) 49
BisI GCNGC 2 cut(s) 44, 203
BlsI GCNGC 2 cut(s) 45, 204
Bme1390I CCNGG 2 cut(s) 536, 546
Bme18I GGWCC 2 cut(s) 6, 243
BmgT120I GGNCC 2 cut(s) 6, 243
BmiI GGNNCC 1 cut(s) 244
BmrFI CCNGG 2 cut(s) 536, 546
BmsI GCATC 1 cut(s) 97
BsaWI WCCGGW 1 cut(s) 19
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 262
BseBI CCWGG 2 cut(s) 536, 546
BseGI GGATG 1 cut(s) 448
BseLI CCNNNNNNNGG 2 cut(s) 91, 262
BseXI GCAGC 1 cut(s) 55
Bsh1236I CGCG 1 cut(s) 202
BshFI GGCC 2 cut(s) 205, 266
BsiSI CCGG 1 cut(s) 20
BslFI GGGAC 1 cut(s) 602
BslI CCNNNNNNNGG 2 cut(s) 91, 262
BsmAI GTCTC 2 cut(s) 415, 530
BsmFI GGGAC 1 cut(s) 602
BsnI GGCC 2 cut(s) 205, 266
Bsp1286I GDGCHC 2 cut(s) 122, 656
Bsp143I GATC 2 cut(s) 487, 559
BspACI CCGC 3 cut(s) 73, 110, 202
BspANI GGCC 2 cut(s) 205, 266
BspFNI CGCG 1 cut(s) 202
BspLI GGNNCC 1 cut(s) 244
BspPI GGATC 1 cut(s) 482
BssMI GATC 2 cut(s) 487, 559
Bst2UI CCWGG 2 cut(s) 536, 546
Bst4CI ACNGT 1 cut(s) 187
Bst6I CTCTTC 1 cut(s) 284
BstC8I GCNNGC 1 cut(s) 122
BstF5I GGATG 1 cut(s) 448
BstFNI CGCG 1 cut(s) 202
BstH2I RGCGCY 1 cut(s) 49
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 2 cut(s) 490, 562
BstMAI GTCTC 2 cut(s) 415, 530
BstMBI GATC 2 cut(s) 487, 559
BstMWI GCNNNNNNNGC 2 cut(s) 130, 374
BstNI CCWGG 2 cut(s) 536, 546
BstNSI RCATGY 1 cut(s) 554
BstSCI CCNGG 2 cut(s) 534, 544
BstSFI CTRYAG 2 cut(s) 297, 351
BstUI CGCG 1 cut(s) 202
BstV1I GCAGC 1 cut(s) 55
BstXI CCANNNNNNTGG 1 cut(s) 572
BsuRI GGCC 2 cut(s) 205, 266
BtsCI GGATG 1 cut(s) 448
BtsI GCAGTG 1 cut(s) 453
BtsIMutI CAGTG 3 cut(s) 246, 327, 453
Cac8I GCNNGC 1 cut(s) 122
CaiI CAGNNNCTG 1 cut(s) 157
CfoI GCGC 1 cut(s) 48
Cfr13I GGNCC 2 cut(s) 6, 243
CviAII CATG 5 cut(s) 125, 381, 551, 566, 598
CviJI RGCY 9 cut(s) 120, 157, 205, 266, 502, 518, 534, 582, 654
CviKI_1 RGCY 9 cut(s) 120, 157, 205, 266, 502, 518, 534, 582, 654
DpnI GATC 2 cut(s) 489, 561
DpnII GATC 2 cut(s) 487, 559
DraIII CACNNNGTG 1 cut(s) 365
EaeI YGGCCR 1 cut(s) 203
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
Eco24I GRGCYC 2 cut(s) 122, 656
Eco47I GGWCC 2 cut(s) 6, 243
Eco47III AGCGCT 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 179
EcoRII CCWGG 2 cut(s) 534, 544
EcoT38I GRGCYC 2 cut(s) 122, 656
FaeI CATG 5 cut(s) 128, 384, 554, 569, 601
FaiI YATR 9 cut(s) 126, 335, 353, 382, 530, 552, 558, 567, 599
FaqI GGGAC 1 cut(s) 602
FatI CATG 5 cut(s) 124, 380, 550, 565, 597
FauI CCCGC 1 cut(s) 80
FbaI TGATCA 1 cut(s) 559
Fnu4HI GCNGC 2 cut(s) 44, 203
FokI GGATG 1 cut(s) 455
FriOI GRGCYC 2 cut(s) 122, 656
Fsp4HI GCNGC 2 cut(s) 44, 203
GlaI GCGC 1 cut(s) 47
GluI GCNGC 2 cut(s) 44, 203
HaeII RGCGCY 1 cut(s) 49
HaeIII GGCC 2 cut(s) 205, 266
HapII CCGG 1 cut(s) 20
HhaI GCGC 1 cut(s) 48
Hin1II CATG 5 cut(s) 128, 384, 554, 569, 601
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HinfI GANTC 1 cut(s) 627
HpaII CCGG 1 cut(s) 20
HphI GGTGA 4 cut(s) 205, 383, 497, 554
Hpy166II GTNNAC 1 cut(s) 246
Hpy188I TCNGA 2 cut(s) 139, 228
Hpy188III TCNNGA 3 cut(s) 146, 171, 386
Hpy8I GTNNAC 1 cut(s) 246
HpyAV CCTTC 2 cut(s) 507, 638
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4V TGCA 3 cut(s) 98, 473, 594
HpyF10VI GCNNNNNNNGC 2 cut(s) 130, 374
Hsp92II CATG 5 cut(s) 128, 384, 554, 569, 601
HspAI GCGC 1 cut(s) 46
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 2 cut(s) 487, 559
Lsp1109I GCAGC 1 cut(s) 55
LweI GCATC 1 cut(s) 97
MalI GATC 2 cut(s) 489, 561
MboI GATC 2 cut(s) 487, 559
MboII GAAGA 5 cut(s) 48, 60, 113, 301, 629
MhlI GDGCHC 2 cut(s) 122, 656
MluCI AATT 3 cut(s) 166, 258, 317
MlyI GAGTC 1 cut(s) 636
MmeI TCCRAC 1 cut(s) 604
MnlI CCTC 7 cut(s) 147, 256, 269, 476, 595, 628, 662
MseI TTAA 2 cut(s) 236, 321
MslI CAYNNNNRTG 2 cut(s) 555, 570
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 2 cut(s) 536, 546
MvaI CCWGG 2 cut(s) 536, 546
MvnI CGCG 1 cut(s) 202
MwoI GCNNNNNNNGC 2 cut(s) 130, 374
NdeII GATC 2 cut(s) 487, 559
NlaIII CATG 5 cut(s) 128, 384, 554, 569, 601
NlaIV GGNNCC 1 cut(s) 244
NspI RCATGY 1 cut(s) 554
PciI ACATGT 1 cut(s) 550
PkrI GCNGC 2 cut(s) 45, 204
PleI GAGTC 1 cut(s) 635
PpsI GAGTC 1 cut(s) 635
PscI ACATGT 1 cut(s) 550
Psp6I CCWGG 2 cut(s) 534, 544
PspGI CCWGG 2 cut(s) 534, 544
PspN4I GGNNCC 1 cut(s) 244
PspPI GGNCC 2 cut(s) 6, 243
PstNI CAGNNNCTG 1 cut(s) 157
RseI CAYNNNNRTG 2 cut(s) 555, 570
SaqAI TTAA 2 cut(s) 236, 321
SatI GCNGC 2 cut(s) 44, 203
Sau3AI GATC 2 cut(s) 487, 559
Sau96I GGNCC 2 cut(s) 6, 243
SchI GAGTC 1 cut(s) 636
ScrFI CCNGG 2 cut(s) 536, 546
SduI GDGCHC 2 cut(s) 122, 656
SetI ASST 6 cut(s) 8, 96, 195, 487, 504, 639
SfaNI GCATC 1 cut(s) 97
SfcI CTRYAG 2 cut(s) 297, 351
SinI GGWCC 2 cut(s) 6, 243
SmiMI CAYNNNNRTG 2 cut(s) 555, 570
Sse9I AATT 3 cut(s) 166, 258, 317
SsiI CCGC 3 cut(s) 73, 110, 202
StyD4I CCNGG 2 cut(s) 534, 544
TaaI ACNGT 1 cut(s) 187
TasI AATT 3 cut(s) 166, 258, 317
TauI GCSGC 1 cut(s) 205
Tru1I TTAA 2 cut(s) 236, 321
Tru9I TTAA 2 cut(s) 236, 321
TscAI CASTG 3 cut(s) 253, 334, 460
TseI GCWGC 1 cut(s) 43
TspDTI ATGAA 3 cut(s) 48, 582, 630
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 3 cut(s) 253, 334, 460
VpaK11BI GGWCC 2 cut(s) 6, 243
XapI RAATTY 1 cut(s) 317
XceI RCATGY 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.