Rroxscaffold_5G00333450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0003902
Physical Location & Seq
Forward (+)
916422 .. 920031
3610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00333450.1

Sequence Viewer

Length: 825 bp
ATGAGTTCCAAGAAAACTCCGGTTGGGTTTAGGGTTTTGGGGCAGCGTTCACTTCCTTCATCATTTTCTTCTCGCTGTAAAATCCCTGTCAAGGCTGCAGAAGATGATGTGGATAAGGGCTCGCATGGGTCGCTCTCGGACATTCCAGAACAGAGGCTGAAGCACAATTCTGGAACACCCAAGATAGTAAAGGTGAAGTCGCGGCCATTCTCGTCGTTATTGGGTCTGAAAGATCTAAGTGGGTCCACTGATGAGCAAATTGAGGCCAATAAAGGAGGGGAAGCAGAGAAGAGTTCTGTAGCATTGGCATTTGAAAAATTTAAGCATACTGACATAGGAAAAGGGCAATCTATAGTTTCACAGAGTGCTGGTGAAGTAGAATGTCCTGACCAAGATGATCTACCAGAATCAAGAAAAAGGAGACACCCTTGTAAAGGTCTTATGCCTTTGAATGCACTAACTCTCAAGCAAGATAATTTACACTCGTGCTATATGATTTTTTACCCACTCACTTCTTTGGTTTATAATTCTGACTTCTCTACCCCTCCTGCTCTTTCAAATGTCAACTATTTAGATGTGGATGACAAACACACTGCGAGAAAGTATTTTGCAGTTCTTGGAGGTGATCCAAAACCCAAGCTAAAAGGGAGAAGGGCTGAGAGTCTCATAAGCCAGGAGAAACCAGGACATGTTTATGATCACTATGAAAATGGTTGTGGCTGGTGGGACTGCAATATGGAGGGTATTGACAATGAAGAAGTTGGTGTCAATGAGGTGTGGGAAGGAGTGGGCTCAACCACATTGGGAGGAATAGAGTGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

274

Amino Acids

30.15

Weight (kDa)

6.32

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
AccII CGCG 1 cut(s) 202
AciI CCGC 1 cut(s) 202
AclWI GGATC 1 cut(s) 620
AcoI YGGCCR 1 cut(s) 203
AcsI RAATTY 1 cut(s) 317
AcuI CTGAAG 1 cut(s) 179
AdeI CACNNNGTG 1 cut(s) 365
AfiI CCNNNNNNNGG 2 cut(s) 91, 434
AflIII ACRYGT 1 cut(s) 688
AgsI TTSAA 3 cut(s) 314, 451, 558
AjnI CCWGG 2 cut(s) 672, 682
AluBI AGCT 1 cut(s) 640
AluI AGCT 1 cut(s) 640
Alw26I GTCTC 2 cut(s) 415, 668
AlwI GGATC 1 cut(s) 620
AlwNI CAGNNNCTG 1 cut(s) 157
AoxI GGCC 2 cut(s) 203, 264
ApeKI GCWGC 2 cut(s) 43, 95
ApoI RAATTY 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 243
AsuHPI GGTGA 3 cut(s) 205, 383, 635
AvaII GGWCC 1 cut(s) 243
BanII GRGCYC 2 cut(s) 122, 794
BauI CACGAG 1 cut(s) 484
BbvI GCAGC 2 cut(s) 55, 82
BciT130I CCWGG 2 cut(s) 674, 684
BclI TGATCA 1 cut(s) 697
BcoDI GTCTC 2 cut(s) 415, 668
BfmI CTRYAG 3 cut(s) 96, 297, 351
BglII AGATCT 1 cut(s) 232
BisI GCNGC 3 cut(s) 44, 96, 203
BlsI GCNGC 3 cut(s) 45, 97, 204
Bme1390I CCNGG 2 cut(s) 674, 684
Bme18I GGWCC 1 cut(s) 243
BmgT120I GGNCC 1 cut(s) 243
BmiI GGNNCC 1 cut(s) 244
BmrFI CCNGG 2 cut(s) 674, 684
BpuEI CTTGAG 1 cut(s) 449
BsaWI WCCGGW 1 cut(s) 19
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 434
BseBI CCWGG 2 cut(s) 674, 684
BseGI GGATG 1 cut(s) 586
BseLI CCNNNNNNNGG 2 cut(s) 91, 434
BseMII CTCAG 1 cut(s) 648
BseXI GCAGC 2 cut(s) 55, 82
Bsh1236I CGCG 1 cut(s) 202
BshFI GGCC 2 cut(s) 205, 266
BsiSI CCGG 1 cut(s) 20
BslFI GGGAC 1 cut(s) 740
BslI CCNNNNNNNGG 2 cut(s) 91, 434
BsmAI GTCTC 2 cut(s) 415, 668
BsmFI GGGAC 1 cut(s) 740
BsmI GAATGC 1 cut(s) 457
BsnI GGCC 2 cut(s) 205, 266
Bsp1286I GDGCHC 2 cut(s) 122, 794
Bsp143I GATC 4 cut(s) 232, 397, 625, 697
BspACI CCGC 1 cut(s) 202
BspANI GGCC 2 cut(s) 205, 266
BspCNI CTCAG 1 cut(s) 649
BspFNI CGCG 1 cut(s) 202
BspLI GGNNCC 1 cut(s) 244
BspMAI CTGCAG 1 cut(s) 100
BspPI GGATC 1 cut(s) 620
BssMI GATC 4 cut(s) 232, 397, 625, 697
BssSI CACGAG 1 cut(s) 484
Bst2BI CACGAG 1 cut(s) 484
Bst2UI CCWGG 2 cut(s) 674, 684
Bst6I CTCTTC 1 cut(s) 284
BstC8I GCNNGC 1 cut(s) 122
BstDEI CTNAG 2 cut(s) 236, 657
BstENI CCTNNNNNAGG 1 cut(s) 432
BstF5I GGATG 1 cut(s) 586
BstFNI CGCG 1 cut(s) 202
BstKTI GATC 4 cut(s) 235, 400, 628, 700
BstMAI GTCTC 2 cut(s) 415, 668
BstMBI GATC 4 cut(s) 232, 397, 625, 697
BstMWI GCNNNNNNNGC 1 cut(s) 130
BstNI CCWGG 2 cut(s) 674, 684
BstNSI RCATGY 1 cut(s) 692
BstSCI CCNGG 2 cut(s) 672, 682
BstSFI CTRYAG 3 cut(s) 96, 297, 351
BstUI CGCG 1 cut(s) 202
BstV1I GCAGC 2 cut(s) 55, 82
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
BsuRI GGCC 2 cut(s) 205, 266
BtsCI GGATG 1 cut(s) 586
BtsI GCAGTG 1 cut(s) 591
BtsIMutI CAGTG 2 cut(s) 246, 591
Cac8I GCNNGC 1 cut(s) 122
CaiI CAGNNNCTG 1 cut(s) 157
Cfr13I GGNCC 1 cut(s) 243
CviAII CATG 2 cut(s) 125, 689
DdeI CTNAG 2 cut(s) 236, 657
DpnI GATC 4 cut(s) 234, 399, 627, 699
DpnII GATC 4 cut(s) 232, 397, 625, 697
DraIII CACNNNGTG 1 cut(s) 365
EaeI YGGCCR 1 cut(s) 203
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
Eco24I GRGCYC 2 cut(s) 122, 794
Eco47I GGWCC 1 cut(s) 243
Eco57I CTGAAG 1 cut(s) 179
EcoNI CCTNNNNNAGG 1 cut(s) 432
EcoRII CCWGG 2 cut(s) 672, 682
EcoT38I GRGCYC 2 cut(s) 122, 794
FaeI CATG 2 cut(s) 128, 692
FaqI GGGAC 1 cut(s) 740
FatI CATG 2 cut(s) 124, 688
FbaI TGATCA 1 cut(s) 697
Fnu4HI GCNGC 3 cut(s) 44, 96, 203
FokI GGATG 1 cut(s) 593
FriOI GRGCYC 2 cut(s) 122, 794
Fsp4HI GCNGC 3 cut(s) 44, 96, 203
GluI GCNGC 3 cut(s) 44, 96, 203
HaeIII GGCC 2 cut(s) 205, 266
HapII CCGG 1 cut(s) 20
Hin1II CATG 2 cut(s) 128, 692
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HinfI GANTC 2 cut(s) 407, 661
HpaII CCGG 1 cut(s) 20
HphI GGTGA 3 cut(s) 205, 383, 635
Hpy166II GTNNAC 3 cut(s) 50, 246, 565
Hpy188I TCNGA 3 cut(s) 139, 228, 532
Hpy188III TCNNGA 4 cut(s) 146, 171, 386, 411
Hpy8I GTNNAC 3 cut(s) 50, 246, 565
Hpy99I CGWCG 1 cut(s) 217
HpyAV CCTTC 3 cut(s) 66, 645, 776
HpyCH4V TGCA 4 cut(s) 98, 455, 611, 732
HpyF10VI GCNNNNNNNGC 1 cut(s) 130
HpyF3I CTNAG 2 cut(s) 236, 657
Hsp92II CATG 2 cut(s) 128, 692
Ksp22I TGATCA 1 cut(s) 697
Kzo9I GATC 4 cut(s) 232, 397, 625, 697
Lsp1109I GCAGC 2 cut(s) 55, 82
MalI GATC 4 cut(s) 234, 399, 627, 699
MboI GATC 4 cut(s) 232, 397, 625, 697
MboII GAAGA 4 cut(s) 60, 113, 301, 767
MflI RGATCY 1 cut(s) 232
MhlI GDGCHC 2 cut(s) 122, 794
MluCI AATT 5 cut(s) 166, 258, 317, 475, 526
MlyI GAGTC 1 cut(s) 670
MnlI CCTC 8 cut(s) 147, 256, 269, 555, 614, 733, 766, 800
MseI TTAA 1 cut(s) 321
MslI CAYNNNNRTG 1 cut(s) 693
MspI CCGG 1 cut(s) 20
MspR9I CCNGG 2 cut(s) 674, 684
Mva1269I GAATGC 1 cut(s) 457
MvaI CCWGG 2 cut(s) 674, 684
MvnI CGCG 1 cut(s) 202
MwoI GCNNNNNNNGC 1 cut(s) 130
NdeII GATC 4 cut(s) 232, 397, 625, 697
NlaIII CATG 2 cut(s) 128, 692
NlaIV GGNNCC 1 cut(s) 244
NspI RCATGY 1 cut(s) 692
PciI ACATGT 1 cut(s) 688
PctI GAATGC 1 cut(s) 457
PfeI GAWTC 1 cut(s) 407
PkrI GCNGC 3 cut(s) 45, 97, 204
PleI GAGTC 1 cut(s) 669
PpsI GAGTC 1 cut(s) 669
PscI ACATGT 1 cut(s) 688
PsiI TTATAA 1 cut(s) 525
Psp6I CCWGG 2 cut(s) 672, 682
PspGI CCWGG 2 cut(s) 672, 682
PspN4I GGNNCC 1 cut(s) 244
PspPI GGNCC 1 cut(s) 243
PstI CTGCAG 1 cut(s) 100
PstNI CAGNNNCTG 1 cut(s) 157
PsuI RGATCY 1 cut(s) 232
RseI CAYNNNNRTG 1 cut(s) 693
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 3 cut(s) 44, 96, 203
Sau3AI GATC 4 cut(s) 232, 397, 625, 697
Sau96I GGNCC 1 cut(s) 243
SchI GAGTC 1 cut(s) 670
ScrFI CCNGG 2 cut(s) 674, 684
SduI GDGCHC 2 cut(s) 122, 794
SetI ASST 5 cut(s) 195, 439, 625, 642, 777
SfcI CTRYAG 3 cut(s) 96, 297, 351
SinI GGWCC 1 cut(s) 243
SmiMI CAYNNNNRTG 1 cut(s) 693
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse9I AATT 5 cut(s) 166, 258, 317, 475, 526
SsiI CCGC 1 cut(s) 202
StyD4I CCNGG 2 cut(s) 672, 682
TasI AATT 5 cut(s) 166, 258, 317, 475, 526
TauI GCSGC 1 cut(s) 205
TfiI GAWTC 1 cut(s) 407
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 2 cut(s) 253, 598
TseI GCWGC 2 cut(s) 43, 95
TspDTI ATGAA 3 cut(s) 48, 720, 768
TspRI CASTG 2 cut(s) 253, 598
VpaK11BI GGWCC 1 cut(s) 243
XagI CCTNNNNNAGG 1 cut(s) 432
XapI RAATTY 1 cut(s) 317
XceI RCATGY 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.