Rh4BG004200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0003902
Physical Location & Seq
Forward (+)
729512 .. 798834
69323 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG004200.1

Sequence Viewer

Length: 675 bp
ATGAGGTCCAAGAAAACTCCGCTTGGGTTTCGGGTTTTGGGGCAGCGCTCACTTTCTTCTCGCAGTAAAATCCCTGTCAAGGTCGCAGAAGATTTTGCGGATAAGGGCTCGCATGGGTCGCTCTCGGACATTCCAGAACAGAGGCTGAAGCACAATTCTGGAACACCCAAGACAGTAAAGGTGAAGTCGCGGCCATTCTCGTCGTTATTGGGTCTGAAAGATCAAAGTGGGTCCACTGATGAGCAAATTGAGGCCAATAAAGGAGGGGAAGCAGAGAAGAGTTCTGTAGCATTGGCATTTGAAAAGTTTAAGTACACTGATATGGGAAAAGGGCAATCTATAGTTTCACAAAGTGCTGGTGAAGTAGAATGTCCTGACCAAGATGATTTACCAGAATCAAGAAAAAGGAGACACCCTTGTAAAGATGGGGATGACAAACACACTGCGAGAAAGTATTTTGCAGTTCTTGGAGGTGATCCAAAACCCAAGCTAAAAGGGAGAAGGGCTGAGAGTCTCATAGGCCAGGAGAAACCAGGACATGTTTATGATCACTATGAAAATGGTTGTGGCTGGTGGGACTGCAATATGGAGGGTATTGACAATGAAGAAGTTGGTGTCAATGAGGTGTGGGAAGGAGTGGGCTCAACCACATTGGGAGGAATAGAGTGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

224

Amino Acids

24.64

Weight (kDa)

7.05

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 190
AciI CCGC 3 cut(s) 20, 98, 190
AclWI GGATC 1 cut(s) 470
AcoI YGGCCR 1 cut(s) 191
AcuI CTGAAG 1 cut(s) 167
AdeI CACNNNGTG 1 cut(s) 353
AfaI GTAC 1 cut(s) 314
AfeI AGCGCT 1 cut(s) 47
AfiI CCNNNNNNNGG 1 cut(s) 79
AflIII ACRYGT 1 cut(s) 538
AgsI TTSAA 1 cut(s) 302
AjnI CCWGG 2 cut(s) 522, 532
AluBI AGCT 1 cut(s) 490
AluI AGCT 1 cut(s) 490
Alw26I GTCTC 2 cut(s) 403, 518
AlwI GGATC 1 cut(s) 470
AlwNI CAGNNNCTG 1 cut(s) 145
Aor51HI AGCGCT 1 cut(s) 47
AoxI GGCC 3 cut(s) 191, 252, 520
ApeKI GCWGC 1 cut(s) 43
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 2 cut(s) 6, 231
AsuHPI GGTGA 3 cut(s) 193, 371, 485
AvaII GGWCC 2 cut(s) 6, 231
BanII GRGCYC 2 cut(s) 110, 644
BbvI GCAGC 1 cut(s) 55
BccI CCATC 1 cut(s) 419
BciT130I CCWGG 2 cut(s) 524, 534
BclI TGATCA 1 cut(s) 547
BcoDI GTCTC 2 cut(s) 403, 518
BfmI CTRYAG 2 cut(s) 285, 339
BfoI RGCGCY 1 cut(s) 49
BisI GCNGC 2 cut(s) 44, 191
BlsI GCNGC 2 cut(s) 45, 192
Bme1390I CCNGG 2 cut(s) 524, 534
Bme18I GGWCC 2 cut(s) 6, 231
BmgT120I GGNCC 2 cut(s) 6, 231
BmiI GGNNCC 1 cut(s) 232
BmrFI CCNGG 2 cut(s) 524, 534
Bsc4I CCNNNNNNNGG 1 cut(s) 79
BseBI CCWGG 2 cut(s) 524, 534
BseGI GGATG 1 cut(s) 436
BseLI CCNNNNNNNGG 1 cut(s) 79
BseMII CTCAG 1 cut(s) 498
BseXI GCAGC 1 cut(s) 55
Bsh1236I CGCG 1 cut(s) 190
BshFI GGCC 3 cut(s) 193, 254, 522
BslFI GGGAC 1 cut(s) 590
BslI CCNNNNNNNGG 1 cut(s) 79
BsmAI GTCTC 2 cut(s) 403, 518
BsmFI GGGAC 1 cut(s) 590
BsnI GGCC 3 cut(s) 193, 254, 522
Bsp1286I GDGCHC 2 cut(s) 110, 644
Bsp143I GATC 3 cut(s) 220, 475, 547
BspACI CCGC 3 cut(s) 20, 98, 190
BspANI GGCC 3 cut(s) 193, 254, 522
BspCNI CTCAG 1 cut(s) 499
BspFNI CGCG 1 cut(s) 190
BspLI GGNNCC 1 cut(s) 232
BspPI GGATC 1 cut(s) 470
BssMI GATC 3 cut(s) 220, 475, 547
Bst2UI CCWGG 2 cut(s) 524, 534
Bst4CI ACNGT 1 cut(s) 175
Bst6I CTCTTC 1 cut(s) 272
BstC8I GCNNGC 1 cut(s) 110
BstDEI CTNAG 1 cut(s) 507
BstF5I GGATG 1 cut(s) 436
BstFNI CGCG 1 cut(s) 190
BstH2I RGCGCY 1 cut(s) 49
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 3 cut(s) 223, 478, 550
BstMAI GTCTC 2 cut(s) 403, 518
BstMBI GATC 3 cut(s) 220, 475, 547
BstMWI GCNNNNNNNGC 1 cut(s) 118
BstNI CCWGG 2 cut(s) 524, 534
BstNSI RCATGY 1 cut(s) 542
BstSCI CCNGG 2 cut(s) 522, 532
BstSFI CTRYAG 2 cut(s) 285, 339
BstUI CGCG 1 cut(s) 190
BstV1I GCAGC 1 cut(s) 55
BsuRI GGCC 3 cut(s) 193, 254, 522
BtsCI GGATG 1 cut(s) 436
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 3 cut(s) 234, 315, 441
Cac8I GCNNGC 1 cut(s) 110
CaiI CAGNNNCTG 1 cut(s) 145
CfoI GCGC 1 cut(s) 48
Cfr13I GGNCC 2 cut(s) 6, 231
Csp6I GTAC 1 cut(s) 313
CviAII CATG 2 cut(s) 113, 539
CviJI RGCY 9 cut(s) 108, 145, 193, 254, 490, 506, 522, 570, 642
CviKI_1 RGCY 9 cut(s) 108, 145, 193, 254, 490, 506, 522, 570, 642
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 1 cut(s) 507
DpnI GATC 3 cut(s) 222, 477, 549
DpnII GATC 3 cut(s) 220, 475, 547
DraIII CACNNNGTG 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 191
Eam1104I CTCTTC 1 cut(s) 272
EarI CTCTTC 1 cut(s) 272
Eco24I GRGCYC 2 cut(s) 110, 644
Eco47I GGWCC 2 cut(s) 6, 231
Eco47III AGCGCT 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 167
EcoRII CCWGG 2 cut(s) 522, 532
EcoT38I GRGCYC 2 cut(s) 110, 644
FaeI CATG 2 cut(s) 116, 542
FaiI YATR 8 cut(s) 114, 323, 341, 518, 540, 546, 555, 587
FaqI GGGAC 1 cut(s) 590
FatI CATG 2 cut(s) 112, 538
FbaI TGATCA 1 cut(s) 547
Fnu4HI GCNGC 2 cut(s) 44, 191
FokI GGATG 1 cut(s) 443
FriOI GRGCYC 2 cut(s) 110, 644
Fsp4HI GCNGC 2 cut(s) 44, 191
GlaI GCGC 1 cut(s) 47
GluI GCNGC 2 cut(s) 44, 191
HaeII RGCGCY 1 cut(s) 49
HaeIII GGCC 3 cut(s) 193, 254, 522
HhaI GCGC 1 cut(s) 48
Hin1II CATG 2 cut(s) 116, 542
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HinfI GANTC 2 cut(s) 395, 511
HphI GGTGA 3 cut(s) 193, 371, 485
Hpy166II GTNNAC 2 cut(s) 234, 315
Hpy188I TCNGA 2 cut(s) 127, 216
Hpy188III TCNNGA 4 cut(s) 134, 159, 374, 399
Hpy8I GTNNAC 2 cut(s) 234, 315
Hpy99I CGWCG 1 cut(s) 205
HpyAV CCTTC 2 cut(s) 495, 626
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4V TGCA 2 cut(s) 461, 582
HpyF10VI GCNNNNNNNGC 1 cut(s) 118
HpyF3I CTNAG 1 cut(s) 507
Hsp92II CATG 2 cut(s) 116, 542
HspAI GCGC 1 cut(s) 46
Ksp22I TGATCA 1 cut(s) 547
Kzo9I GATC 3 cut(s) 220, 475, 547
Lsp1109I GCAGC 1 cut(s) 55
MalI GATC 3 cut(s) 222, 477, 549
MboI GATC 3 cut(s) 220, 475, 547
MboII GAAGA 4 cut(s) 48, 101, 289, 617
MhlI GDGCHC 2 cut(s) 110, 644
MluCI AATT 2 cut(s) 154, 246
MlyI GAGTC 1 cut(s) 520
MnlI CCTC 7 cut(s) 135, 244, 257, 464, 583, 616, 650
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 2 cut(s) 320, 543
MspR9I CCNGG 2 cut(s) 524, 534
MvaI CCWGG 2 cut(s) 524, 534
MvnI CGCG 1 cut(s) 190
MwoI GCNNNNNNNGC 1 cut(s) 118
NdeII GATC 3 cut(s) 220, 475, 547
NlaIII CATG 2 cut(s) 116, 542
NlaIV GGNNCC 1 cut(s) 232
NspI RCATGY 1 cut(s) 542
PciI ACATGT 1 cut(s) 538
PfeI GAWTC 1 cut(s) 395
PkrI GCNGC 2 cut(s) 45, 192
PleI GAGTC 1 cut(s) 519
PpsI GAGTC 1 cut(s) 519
PscI ACATGT 1 cut(s) 538
Psp6I CCWGG 2 cut(s) 522, 532
PspGI CCWGG 2 cut(s) 522, 532
PspN4I GGNNCC 1 cut(s) 232
PspPI GGNCC 2 cut(s) 6, 231
PstNI CAGNNNCTG 1 cut(s) 145
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
RseI CAYNNNNRTG 2 cut(s) 320, 543
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 2 cut(s) 44, 191
Sau3AI GATC 3 cut(s) 220, 475, 547
Sau96I GGNCC 2 cut(s) 6, 231
SchI GAGTC 1 cut(s) 520
ScrFI CCNGG 2 cut(s) 524, 534
SduI GDGCHC 2 cut(s) 110, 644
SetI ASST 6 cut(s) 8, 84, 183, 475, 492, 627
SfcI CTRYAG 2 cut(s) 285, 339
SinI GGWCC 2 cut(s) 6, 231
SmiMI CAYNNNNRTG 2 cut(s) 320, 543
Sse9I AATT 2 cut(s) 154, 246
SsiI CCGC 3 cut(s) 20, 98, 190
StyD4I CCNGG 2 cut(s) 522, 532
TaaI ACNGT 1 cut(s) 175
TasI AATT 2 cut(s) 154, 246
TatI WGTACW 1 cut(s) 312
TauI GCSGC 1 cut(s) 193
TfiI GAWTC 1 cut(s) 395
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 3 cut(s) 241, 322, 448
TseI GCWGC 1 cut(s) 43
TspDTI ATGAA 2 cut(s) 570, 618
TspRI CASTG 3 cut(s) 241, 322, 448
VpaK11BI GGWCC 2 cut(s) 6, 231
XceI RCATGY 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.