RchiOBHm_Chr1g0318301

Protein of unknown function (DUF616)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0002998
Physical Location & Seq
Reverse (-)
5779441 .. 5783257
3817 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1422 bp
ATGTTCAACAGCAACAGCGTATCAATCTCAGTCTCCGATGACGAATCCGACGAGCTCGGCCGGATGCGCGTCCGAGTTCGCCGGAAGCGCAAGAAACTCGGCCACCGATTCAAGAACGAGTTCGTTCGCCGAGTGGTGAGGAATCTGGTCAGGTACTGGGCCCTTCTGATTCTTCTGCCAGCCATTGGTTTGCTTCTGTATGAGGCTTGGAGTATCGGCTGGAAATCCCCACAGCTCAATAGTAAGTCCGAAAAGCCGAGTTTGGTCCACTCGGAACTCAATGCGAGTTCGGAGAAGAAATCCGAAGGCAATTTGAATCGGCTTGATCCTATCACTCGGGTTATTCATGGCGTTAGGGAACGTTGCTTGCAAATCTTACCTCCTGAAGAACTTGAGCATCTGGAAATTCCTGTGCGTGAAGATGCCACTAGTCCTGTTAAGAAATTGTTATACAGAGCGCAGAATGATGGTCTGTTGTTAGGAGGGTACAACACCCGGTCTGAAGAGCACACAGAGGCCACAAGATTTAATTTGTTTACGGGAAATCAAACTCTAGATCAGAGAGGTGAAATGTTTAAGGTAAATGAAACTGTTACCATAAACTGTGGTTTCTACAGTGAAAATGGTGGGTTTAAGATCTCAAATGAAGACAAGAATTACATGCAAAGTTGTGACATTGTAGTCTCCACTTGTGCATTTGGTGGTGGAGATGATCTTTATCAACCCATTGGAATGTCAGAGGCATCCCTTCAGAAGGTTTGCTATGTTGCTTTTTGGGATGAAGTTACTGTAAGTACTCAGGAATCAGCTGAACATAGAATTGGTGAGGATGGATTTATTGGGAGATGGCGCATTGTGGTTGTAAGCGATCTGCCTTTTGCGGACCAAAGGTTAAATGGAAAAATCCCAAAGATGTTGGCGCATCGCCTTTTTCCTCATTCAAAGTATTCCATTTGGGTAGATTCAAAGTCCCAATTTAGGAGGGACCCTTTAGGTGTTCTCGAAGCCCTTCTCTGGCGTACCAATTCAGTACTTGCGATCTCAGAACATGGAGCTCGCAGTAGTGTTTATGATGAGGCAAAGGCCGTTGTCAAGAAAAACAAGGCCAAACCGGAAGAAGTTGAGGTACAGTTGGCTCAGTACCGTCATGATGGATTGCCAGAAGACAAGAGGTTCCATGGAAAGAAAGCTCTTGCCGAAGCATCTGTTATCGTTAGGGAGCATACCCTGTTGACGAATATGTTTATGTGCCTCTGGTTCAACGAGGTGGTTCGTTTCACGTCTCGCGATCAGCTCAGTTTCCCATATGTACTGTGGAGGTTGAAAGTGCTCAAGAACATCAACATGTTCCCTGTATGCACCCGCAAAGATCTTGTTAATAGTATGGGCCACATACGCAAGGCTAAGCCTCTGAAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF616 Dirigent

Protein Analysis

473

Amino Acids

54.27

Weight (kDa)

9.23

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TOD1_MUCI70 PF04765 167 - 464 1.3e-118 TOD1/MUCI70, glycosyltransferase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 680
AccB7I CCANNNNNTGG 1 cut(s) 185
AccII CGCG 2 cut(s) 69, 1287
AciI CCGC 2 cut(s) 881, 1363
AclI AACGTT 1 cut(s) 361
AclWI GGATC 1 cut(s) 320
AcoI YGGCCR 2 cut(s) 58, 100
AcsI RAATTY 2 cut(s) 405, 1416
AcuI CTGAAG 3 cut(s) 405, 522, 734
AfaI GTAC 8 cut(s) 155, 488, 796, 1021, 1032, 1128, 1142, 1311
AfiI CCNNNNNNNGG 4 cut(s) 185, 754, 978, 1014
AflIII ACRYGT 1 cut(s) 1344
AgsI TTSAA 7 cut(s) 7, 112, 316, 942, 966, 1261, 1324
AhlI ACTAGT 1 cut(s) 428
AjiI CACGTC 1 cut(s) 1281
AjuI GAANNNNNNNTTGG 2 cut(s) 804, 836
AluBI AGCT 6 cut(s) 55, 235, 809, 1055, 1190, 1294
AluI AGCT 6 cut(s) 55, 235, 809, 1055, 1190, 1294
Alw21I GWGCWC 4 cut(s) 57, 510, 1057, 1332
Alw26I GTCTC 3 cut(s) 37, 688, 1287
AlwI GGATC 1 cut(s) 320
AlwNI CAGNNNCTG 1 cut(s) 156
Ama87I CYCGRG 1 cut(s) 336
AoxI GGCC 7 cut(s) 58, 100, 159, 516, 1083, 1104, 1387
ApaI GGGCCC 1 cut(s) 163
ApoI RAATTY 2 cut(s) 405, 1416
Asp700I GAANNNNTTC 2 cut(s) 119, 1008
AspLEI GCGC 5 cut(s) 69, 90, 460, 852, 922
AspS9I GGNCC 6 cut(s) 159, 160, 265, 883, 985, 1387
AsuC2I CCSGG 1 cut(s) 496
AsuHPI GGTGA 3 cut(s) 148, 578, 836
AvaI CYCGRG 1 cut(s) 336
AvaII GGWCC 3 cut(s) 265, 883, 985
BaeGI GKGCMC 1 cut(s) 163
BanII GRGCYC 3 cut(s) 57, 163, 1057
BarI GAAGNNNNNNTAC 2 cut(s) 1110, 1142
BbsI GAAGAC 2 cut(s) 654, 1170
Bbv12I GWGCWC 4 cut(s) 57, 510, 1057, 1332
BccI CCATC 4 cut(s) 461, 824, 840, 1145
BceAI ACGGC 1 cut(s) 1070
BcgI CGANNNNNNTGC 2 cut(s) 79, 113
BcnI CCSGG 1 cut(s) 496
BcoDI GTCTC 3 cut(s) 37, 688, 1287
BcuI ACTAGT 1 cut(s) 428
BfaI CTAG 2 cut(s) 429, 554
BfmI CTRYAG 1 cut(s) 613
BglII AGATCT 2 cut(s) 636, 1369
BlpI GCTNAGC 1 cut(s) 1404
BmcAI AGTACT 2 cut(s) 796, 1032
Bme1390I CCNGG 1 cut(s) 496
Bme18I GGWCC 3 cut(s) 265, 883, 985
BmeT110I CYCGRG 1 cut(s) 336
BmgBI CACGTC 1 cut(s) 1281
BmgT120I GGNCC 6 cut(s) 159, 160, 265, 883, 985, 1387
BmiI GGNNCC 4 cut(s) 161, 986, 987, 1175
BmrFI CCNGG 1 cut(s) 496
BmrI ACTGGG 1 cut(s) 166
BmsI GCATC 6 cut(s) 54, 406, 412, 752, 931, 1211
BmuI ACTGGG 1 cut(s) 166
BpiI GAAGAC 2 cut(s) 654, 1170
Bpu1102I GCTNAGC 1 cut(s) 1404
BpuEI CTTGAG 2 cut(s) 413, 1316
BpuMI CCSGG 1 cut(s) 496
BsaBI GATNNNNATC 1 cut(s) 717
BsaJI CCNNGG 1 cut(s) 1177
BsaWI WCCGGW 1 cut(s) 1111
Bsc4I CCNNNNNNNGG 4 cut(s) 185, 754, 978, 1014
Bse1I ACTGG 1 cut(s) 161
Bse8I GATNNNNATC 1 cut(s) 717
BseDI CCNNGG 1 cut(s) 1177
BseGI GGATG 4 cut(s) 69, 743, 784, 835
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 4 cut(s) 185, 754, 978, 1014
BseMII CTCAG 5 cut(s) 42, 812, 1056, 1151, 1309
BseNI ACTGG 1 cut(s) 161
BseSI GKGCMC 1 cut(s) 163
BseX3I CGGCCG 1 cut(s) 58
Bsh1236I CGCG 2 cut(s) 69, 1287
Bsh1285I CGRYCG 1 cut(s) 61
BshFI GGCC 7 cut(s) 60, 102, 161, 518, 1085, 1106, 1389
BsiEI CGRYCG 1 cut(s) 61
BsiHKAI GWGCWC 4 cut(s) 57, 510, 1057, 1332
BsiHKCI CYCGRG 1 cut(s) 336
BsiSI CCGG 4 cut(s) 61, 82, 496, 1112
BslFI GGGAC 2 cut(s) 955, 998
BslI CCNNNNNNNGG 4 cut(s) 185, 754, 978, 1014
BsmAI GTCTC 3 cut(s) 37, 688, 1287
BsmBI CGTCTC 1 cut(s) 1287
BsmFI GGGAC 2 cut(s) 955, 998
BsnI GGCC 7 cut(s) 60, 102, 161, 518, 1085, 1106, 1389
BsoBI CYCGRG 1 cut(s) 336
Bsp120I GGGCCC 1 cut(s) 159
Bsp1286I GDGCHC 5 cut(s) 57, 163, 510, 1057, 1332
Bsp143I GATC 8 cut(s) 325, 556, 636, 712, 868, 1038, 1288, 1369
Bsp1720I GCTNAGC 1 cut(s) 1404
Bsp19I CCATGG 1 cut(s) 1177
Bsp68I TCGCGA 1 cut(s) 1287
BspACI CCGC 2 cut(s) 881, 1363
BspANI GGCC 7 cut(s) 60, 102, 161, 518, 1085, 1106, 1389
BspCNI CTCAG 5 cut(s) 41, 811, 1055, 1150, 1308
BspFNI CGCG 2 cut(s) 69, 1287
BspHI TCATGA 1 cut(s) 1147
BspLI GGNNCC 4 cut(s) 161, 986, 987, 1175
BspPI GGATC 1 cut(s) 320
BspQI GCTCTTC 1 cut(s) 498
BsrI ACTGG 1 cut(s) 161
BssECI CCNNGG 1 cut(s) 1177
BssMI GATC 8 cut(s) 325, 556, 636, 712, 868, 1038, 1288, 1369
BssT1I CCWWGG 1 cut(s) 1177
Bst4CI ACNGT 7 cut(s) 592, 605, 617, 790, 1131, 1145, 1314
Bst6I CTCTTC 1 cut(s) 498
BstC8I GCNNGC 3 cut(s) 180, 368, 1057
BstDEI CTNAG 6 cut(s) 28, 798, 1042, 1137, 1295, 1404
BstDSI CCRYGG 1 cut(s) 1177
BstENI CCTNNNNNAGG 1 cut(s) 752
BstF5I GGATG 4 cut(s) 69, 743, 784, 835
BstFNI CGCG 2 cut(s) 69, 1287
BstHHI GCGC 5 cut(s) 69, 90, 460, 852, 922
BstKTI GATC 8 cut(s) 328, 559, 639, 715, 871, 1041, 1291, 1372
BstMAI GTCTC 3 cut(s) 37, 688, 1287
BstMBI GATC 8 cut(s) 325, 556, 636, 712, 868, 1038, 1288, 1369
BstMCI CGRYCG 1 cut(s) 61
BstMWI GCNNNNNNNGC 3 cut(s) 66, 87, 1395
BstNSI RCATGY 2 cut(s) 664, 1348
BstSCI CCNGG 1 cut(s) 494
BstSFI CTRYAG 1 cut(s) 613
BstSLI GKGCMC 1 cut(s) 163
BstUI CGCG 2 cut(s) 69, 1287
BstV2I GAAGAC 2 cut(s) 654, 1170
BstX2I RGATCY 2 cut(s) 636, 1369
BstYI RGATCY 2 cut(s) 636, 1369
BstZI CGGCCG 1 cut(s) 58
BsuRI GGCC 7 cut(s) 60, 102, 161, 518, 1085, 1106, 1389
BtgI CCRYGG 1 cut(s) 1177
BtgZI GCGATG 1 cut(s) 908
BtrI CACGTC 1 cut(s) 1281
BtsCI GGATG 4 cut(s) 69, 743, 784, 835
BtsIMutI CAGTG 1 cut(s) 622
BtuMI TCGCGA 1 cut(s) 1287
Cac8I GCNNGC 3 cut(s) 180, 368, 1057
CaiI CAGNNNCTG 1 cut(s) 156
CciI TCATGA 1 cut(s) 1147
CfoI GCGC 5 cut(s) 69, 90, 460, 852, 922
Cfr13I GGNCC 6 cut(s) 159, 160, 265, 883, 985, 1387
CseI GACGC 1 cut(s) 58
Csp6I GTAC 8 cut(s) 154, 487, 795, 1020, 1031, 1127, 1141, 1310
CviAII CATG 6 cut(s) 347, 661, 1049, 1148, 1178, 1345
CviQI GTAC 8 cut(s) 154, 487, 795, 1020, 1031, 1127, 1141, 1310
DdeI CTNAG 6 cut(s) 28, 798, 1042, 1137, 1295, 1404
DpnI GATC 8 cut(s) 327, 558, 638, 714, 870, 1040, 1290, 1371
DpnII GATC 8 cut(s) 325, 556, 636, 712, 868, 1038, 1288, 1369
DrdI GACNNNNNNGTC 1 cut(s) 680
DseDI GACNNNNNNGTC 1 cut(s) 680
EaeI YGGCCR 2 cut(s) 58, 100
EagI CGGCCG 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
Ecl136II GAGCTC 2 cut(s) 55, 1055
EclXI CGGCCG 1 cut(s) 58
Eco130I CCWWGG 1 cut(s) 1177
Eco24I GRGCYC 3 cut(s) 57, 163, 1057
Eco47I GGWCC 3 cut(s) 265, 883, 985
Eco52I CGGCCG 1 cut(s) 58
Eco53kI GAGCTC 2 cut(s) 55, 1055
Eco57I CTGAAG 3 cut(s) 405, 522, 734
Eco88I CYCGRG 1 cut(s) 336
EcoICRI GAGCTC 2 cut(s) 55, 1055
EcoNI CCTNNNNNAGG 1 cut(s) 752
EcoO109I RGGNCCY 2 cut(s) 160, 985
EcoT14I CCWWGG 1 cut(s) 1177
EcoT38I GRGCYC 3 cut(s) 57, 163, 1057
ErhI CCWWGG 1 cut(s) 1177
Esp3I CGTCTC 1 cut(s) 1287
FaeI CATG 6 cut(s) 350, 664, 1052, 1151, 1181, 1348
FaqI GGGAC 2 cut(s) 955, 998
FatI CATG 6 cut(s) 346, 660, 1048, 1147, 1177, 1344
FauI CCCGC 1 cut(s) 1370
FauNDI CATATG 1 cut(s) 1306
FokI GGATG 4 cut(s) 76, 730, 791, 842
FriOI GRGCYC 3 cut(s) 57, 163, 1057
FspBI CTAG 2 cut(s) 429, 554
GlaI GCGC 5 cut(s) 68, 89, 459, 851, 921
HaeIII GGCC 7 cut(s) 60, 102, 161, 518, 1085, 1106, 1389
HapII CCGG 4 cut(s) 61, 82, 496, 1112
HgaI GACGC 1 cut(s) 58
HhaI GCGC 5 cut(s) 69, 90, 460, 852, 922
Hin1II CATG 6 cut(s) 350, 664, 1052, 1151, 1181, 1348
Hin6I GCGC 5 cut(s) 67, 88, 458, 850, 920
HinP1I GCGC 5 cut(s) 67, 88, 458, 850, 920
HincII GTYRAC 1 cut(s) 1233
HindII GTYRAC 1 cut(s) 1233
HinfI GANTC 7 cut(s) 44, 108, 142, 169, 316, 803, 962
HpaII CCGG 4 cut(s) 61, 82, 496, 1112
HphI GGTGA 3 cut(s) 148, 578, 836
Hpy166II GTNNAC 3 cut(s) 268, 537, 1233
Hpy8I GTNNAC 3 cut(s) 268, 537, 1233
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 5 cut(s) 173, 299, 748, 758, 1019
HpyCH4III ACNGT 7 cut(s) 592, 605, 617, 790, 1131, 1145, 1314
HpyCH4IV ACGT 2 cut(s) 361, 1280
HpyCH4V TGCA 4 cut(s) 370, 664, 695, 1359
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 87, 1395
HpyF3I CTNAG 6 cut(s) 28, 798, 1042, 1137, 1295, 1404
HpySE526I ACGT 2 cut(s) 361, 1280
Hsp92II CATG 6 cut(s) 350, 664, 1052, 1151, 1181, 1348
HspAI GCGC 5 cut(s) 67, 88, 458, 850, 920
KflI GGGWCCC 1 cut(s) 985
Kzo9I GATC 8 cut(s) 325, 556, 636, 712, 868, 1038, 1288, 1369
LguI GCTCTTC 1 cut(s) 498
LmnI GCTCC 2 cut(s) 1052, 1219
LweI GCATC 6 cut(s) 54, 406, 412, 752, 931, 1211
MaeI CTAG 2 cut(s) 429, 554
MaeII ACGT 2 cut(s) 361, 1280
MaeIII GTNAC 3 cut(s) 592, 671, 784
MalI GATC 8 cut(s) 327, 558, 638, 714, 870, 1040, 1290, 1371
MboI GATC 8 cut(s) 325, 556, 636, 712, 868, 1038, 1288, 1369
MboII GAAGA 8 cut(s) 164, 307, 398, 431, 515, 659, 1127, 1175
MflI RGATCY 2 cut(s) 636, 1369
MhlI GDGCHC 5 cut(s) 57, 163, 510, 1057, 1332
MluCI AATT 9 cut(s) 310, 405, 443, 529, 655, 819, 974, 1024, 1416
MmeI TCCRAC 1 cut(s) 72
MroXI GAANNNNTTC 2 cut(s) 119, 1008
MseI TTAA 6 cut(s) 438, 528, 576, 633, 893, 1377
MslI CAYNNNNRTG 2 cut(s) 731, 1343
MspA1I CMGCKG 1 cut(s) 809
MspI CCGG 4 cut(s) 61, 82, 496, 1112
MspR9I CCNGG 1 cut(s) 496
MvnI CGCG 2 cut(s) 69, 1287
MwoI GCNNNNNNNGC 3 cut(s) 66, 87, 1395
NciI CCSGG 1 cut(s) 496
NcoI CCATGG 1 cut(s) 1177
NdeI CATATG 1 cut(s) 1306
NdeII GATC 8 cut(s) 325, 556, 636, 712, 868, 1038, 1288, 1369
NlaIII CATG 6 cut(s) 350, 664, 1052, 1151, 1181, 1348
NlaIV GGNNCC 4 cut(s) 161, 986, 987, 1175
NmeAIII GCCGAG 4 cut(s) 36, 78, 155, 282
NmuCI GTSAC 1 cut(s) 671
NruI TCGCGA 1 cut(s) 1287
NspI RCATGY 2 cut(s) 664, 1348
PagI TCATGA 1 cut(s) 1147
PciI ACATGT 1 cut(s) 1344
PciSI GCTCTTC 1 cut(s) 498
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PdmI GAANNNNTTC 2 cut(s) 119, 1008
PfeI GAWTC 7 cut(s) 44, 108, 142, 169, 316, 803, 962
PflMI CCANNNNNTGG 1 cut(s) 185
PpuMI RGGWCCY 1 cut(s) 985
PscI ACATGT 1 cut(s) 1344
Psp124BI GAGCTC 2 cut(s) 57, 1057
Psp1406I AACGTT 1 cut(s) 361
Psp5II RGGWCCY 1 cut(s) 985
PspN4I GGNNCC 4 cut(s) 161, 986, 987, 1175
PspOMI GGGCCC 1 cut(s) 159
PspPI GGNCC 6 cut(s) 159, 160, 265, 883, 985, 1387
PspPPI RGGWCCY 1 cut(s) 985
PstNI CAGNNNCTG 1 cut(s) 156
PsuI RGATCY 2 cut(s) 636, 1369
PvuII CAGCTG 1 cut(s) 809
RruI TCGCGA 1 cut(s) 1287
RsaI GTAC 8 cut(s) 155, 488, 796, 1021, 1032, 1128, 1142, 1311
RsaNI GTAC 8 cut(s) 154, 487, 795, 1020, 1031, 1127, 1141, 1310
RseI CAYNNNNRTG 2 cut(s) 731, 1343
SacI GAGCTC 2 cut(s) 57, 1057
SapI GCTCTTC 1 cut(s) 498
SaqAI TTAA 6 cut(s) 438, 528, 576, 633, 893, 1377
Sau3AI GATC 8 cut(s) 325, 556, 636, 712, 868, 1038, 1288, 1369
Sau96I GGNCC 6 cut(s) 159, 160, 265, 883, 985, 1387
ScaI AGTACT 2 cut(s) 796, 1032
ScrFI CCNGG 1 cut(s) 496
SduI GDGCHC 5 cut(s) 57, 163, 510, 1057, 1332
SfaNI GCATC 6 cut(s) 54, 406, 412, 752, 931, 1211
SfcI CTRYAG 1 cut(s) 613
SinI GGWCC 3 cut(s) 265, 883, 985
SmiMI CAYNNNNRTG 2 cut(s) 731, 1343
SmlI CTYRAG 2 cut(s) 392, 1331
SmoI CTYRAG 2 cut(s) 392, 1331
SpeI ACTAGT 1 cut(s) 428
Sse9I AATT 9 cut(s) 310, 405, 443, 529, 655, 819, 974, 1024, 1416
SsiI CCGC 2 cut(s) 881, 1363
SspMI CTAG 2 cut(s) 429, 554
SstI GAGCTC 2 cut(s) 57, 1057
StyD4I CCNGG 1 cut(s) 494
StyI CCWWGG 1 cut(s) 1177
TaaI ACNGT 7 cut(s) 592, 605, 617, 790, 1131, 1145, 1314
TaiI ACGT 2 cut(s) 364, 1283
TaqI TCGA 1 cut(s) 1002
TasI AATT 9 cut(s) 310, 405, 443, 529, 655, 819, 974, 1024, 1416
TatI WGTACW 3 cut(s) 794, 1030, 1309
TfiI GAWTC 7 cut(s) 44, 108, 142, 169, 316, 803, 962
Tru1I TTAA 6 cut(s) 438, 528, 576, 633, 893, 1377
Tru9I TTAA 6 cut(s) 438, 528, 576, 633, 893, 1377
TscAI CASTG 1 cut(s) 622
TseFI GTSAC 1 cut(s) 671
Tsp45I GTSAC 1 cut(s) 671
TspDTI ATGAA 4 cut(s) 335, 600, 660, 795
TspRI CASTG 1 cut(s) 622
Van91I CCANNNNNTGG 1 cut(s) 185
VpaK11BI GGWCC 3 cut(s) 265, 883, 985
XagI CCTNNNNNAGG 1 cut(s) 752
XapI RAATTY 2 cut(s) 405, 1416
XbaI TCTAGA 1 cut(s) 553
XceI RCATGY 2 cut(s) 664, 1348
XcmI CCANNNNNNNNNTGG 2 cut(s) 893, 1311
XmnI GAANNNNTTC 2 cut(s) 119, 1008
XspI CTAG 2 cut(s) 429, 554
ZrmI AGTACT 2 cut(s) 796, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.