RchiOBHm_Chr1g0318721

Protein CWC15 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0006245
Physical Location & Seq
Forward (+)
6255500 .. 6256408
909 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 420 bp
ATGACGACTGCAGCTCGACCCACGAGGGCTCCTGCGAAAGGTGGTAATGAACAAGGTGGTACTCAGATTTTTGGCCCATCCCAAAAGTACTCATCAAGGGACATTGCATCCCATACAACTCTAAAACCCAAAAAGGACAGAAAGGACATCCAGGAAGAACTGCATAAAAGGAACCTCCAGGAGGAGTTGGAAGACTATGAGAGGAGGCATTTTTCATCAAAGGATAAATATAGTTATGACAGAGATCGCAGCAGGAGAGGGAGCCAACTTTTGTTGGAAAGGATAAGAAGGGACACTGAAGACTGCGTTATACCACGTAATGTAGATGCTGATGATTCTGATATGGAAGTCAAAAGTGATGATGATAGGTTAGTATTACCAGCTATATGTGAATCAGACTTTACATATGTTTCCCCGTGA

Protein Analysis

139

Amino Acids

16.08

Weight (kDa)

5.96

Isoelectric Point (pI)

60.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cwf_Cwc_15 PF04889 1 - 121 1.4e-19 Cwf15/Cwc15 cell cycle control protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 318
AfaI GTAC 2 cut(s) 61, 89
AfiI CCNNNNNNNGG 2 cut(s) 38, 181
AjnI CCWGG 2 cut(s) 150, 177
AluBI AGCT 2 cut(s) 14, 383
AluI AGCT 2 cut(s) 14, 383
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 2 cut(s) 11, 249
AspS9I GGNCC 1 cut(s) 74
BanII GRGCYC 1 cut(s) 31
BauI CACGAG 1 cut(s) 22
BbsI GAAGAC 2 cut(s) 198, 306
BbvI GCAGC 2 cut(s) 23, 261
BccI CCATC 1 cut(s) 85
BciT130I CCWGG 2 cut(s) 152, 179
BfmI CTRYAG 1 cut(s) 9
BisI GCNGC 2 cut(s) 12, 250
BlsI GCNGC 2 cut(s) 13, 251
BmcAI AGTACT 1 cut(s) 89
Bme1390I CCNGG 2 cut(s) 152, 179
BmgT120I GGNCC 1 cut(s) 74
BmiI GGNNCC 3 cut(s) 30, 173, 263
BmrFI CCNGG 2 cut(s) 152, 179
BmsI GCATC 2 cut(s) 116, 316
BpiI GAAGAC 2 cut(s) 198, 306
BpmI CTGGAG 1 cut(s) 161
BsaAI YACGTR 1 cut(s) 317
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 181
Bse3DI GCAATG 1 cut(s) 102
BseBI CCWGG 2 cut(s) 152, 179
BseGI GGATG 3 cut(s) 77, 107, 147
BseLI CCNNNNNNNGG 2 cut(s) 38, 181
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 1 cut(s) 77
BseRI GAGGAG 2 cut(s) 197, 217
BseXI GCAGC 2 cut(s) 23, 261
BshFI GGCC 1 cut(s) 75
BslFI GGGAC 2 cut(s) 113, 305
BslI CCNNNNNNNGG 2 cut(s) 38, 181
BsmFI GGGAC 2 cut(s) 113, 305
BsnI GGCC 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 1 cut(s) 244
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 1 cut(s) 76
BspLI GGNNCC 3 cut(s) 30, 173, 263
BspMAI CTGCAG 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 102
BssMI GATC 1 cut(s) 244
BssSI CACGAG 1 cut(s) 22
Bst2BI CACGAG 1 cut(s) 22
Bst2UI CCWGG 2 cut(s) 152, 179
BstBAI YACGTR 1 cut(s) 317
BstDEI CTNAG 1 cut(s) 63
BstENI CCTNNNNNAGG 2 cut(s) 36, 179
BstF5I GGATG 3 cut(s) 77, 107, 147
BstKTI GATC 1 cut(s) 247
BstMBI GATC 1 cut(s) 244
BstNI CCWGG 2 cut(s) 152, 179
BstSCI CCNGG 2 cut(s) 150, 177
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 2 cut(s) 23, 261
BstV2I GAAGAC 2 cut(s) 198, 306
BsuRI GGCC 1 cut(s) 75
BtsCI GGATG 3 cut(s) 77, 107, 147
BtsIMutI CAGTG 1 cut(s) 294
Cfr13I GGNCC 1 cut(s) 74
Csp6I GTAC 2 cut(s) 60, 88
CviJI RGCY 5 cut(s) 14, 29, 75, 264, 383
CviKI_1 RGCY 5 cut(s) 14, 29, 75, 264, 383
CviQI GTAC 2 cut(s) 60, 88
DdeI CTNAG 1 cut(s) 63
DpnI GATC 1 cut(s) 246
DpnII GATC 1 cut(s) 244
Eco24I GRGCYC 1 cut(s) 31
Eco57I CTGAAG 1 cut(s) 318
EcoNI CCTNNNNNAGG 2 cut(s) 36, 179
EcoRII CCWGG 2 cut(s) 150, 177
EcoT38I GRGCYC 1 cut(s) 31
FaqI GGGAC 2 cut(s) 113, 305
FauNDI CATATG 1 cut(s) 406
Fnu4HI GCNGC 2 cut(s) 12, 250
FokI GGATG 3 cut(s) 64, 94, 134
FriOI GRGCYC 1 cut(s) 31
Fsp4HI GCNGC 2 cut(s) 12, 250
GluI GCNGC 2 cut(s) 12, 250
GsuI CTGGAG 1 cut(s) 161
HaeIII GGCC 1 cut(s) 75
HinfI GANTC 2 cut(s) 335, 392
Hpy188I TCNGA 3 cut(s) 66, 340, 397
HpyAV CCTTC 1 cut(s) 282
HpyCH4IV ACGT 1 cut(s) 316
HpyCH4V TGCA 3 cut(s) 11, 107, 163
HpyF3I CTNAG 1 cut(s) 63
HpySE526I ACGT 1 cut(s) 316
Kzo9I GATC 1 cut(s) 244
LmnI GCTCC 2 cut(s) 34, 261
LpnPI CCDG 7 cut(s) 45, 137, 164, 164, 191, 238, 393
Lsp1109I GCAGC 2 cut(s) 23, 261
LweI GCATC 2 cut(s) 116, 316
MaeII ACGT 1 cut(s) 316
MalI GATC 1 cut(s) 246
MboI GATC 1 cut(s) 244
MboII GAAGA 3 cut(s) 167, 203, 311
MhlI GDGCHC 1 cut(s) 31
MmeI TCCRAC 2 cut(s) 168, 255
MnlI CCTC 6 cut(s) 18, 175, 185, 195, 198, 251
MspR9I CCNGG 2 cut(s) 152, 179
MvaI CCWGG 2 cut(s) 152, 179
NdeI CATATG 1 cut(s) 406
NdeII GATC 1 cut(s) 244
NlaIV GGNNCC 3 cut(s) 30, 173, 263
PfeI GAWTC 2 cut(s) 335, 392
PfoI TCCNGGA 2 cut(s) 150, 177
PkrI GCNGC 2 cut(s) 13, 251
Ppu21I YACGTR 1 cut(s) 317
Psp6I CCWGG 2 cut(s) 150, 177
PspGI CCWGG 2 cut(s) 150, 177
PspN4I GGNNCC 3 cut(s) 30, 173, 263
PspPI GGNCC 1 cut(s) 74
PstI CTGCAG 1 cut(s) 13
RsaI GTAC 2 cut(s) 61, 89
RsaNI GTAC 2 cut(s) 60, 88
SatI GCNGC 2 cut(s) 12, 250
Sau3AI GATC 1 cut(s) 244
Sau96I GGNCC 1 cut(s) 74
ScaI AGTACT 1 cut(s) 89
ScrFI CCNGG 2 cut(s) 152, 179
SduI GDGCHC 1 cut(s) 31
SetI ASST 7 cut(s) 16, 43, 58, 177, 319, 371, 385
SfaNI GCATC 2 cut(s) 116, 316
SfcI CTRYAG 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 150, 177
TaiI ACGT 1 cut(s) 319
TaqI TCGA 1 cut(s) 16
TatI WGTACW 1 cut(s) 87
TfiI GAWTC 2 cut(s) 335, 392
TscAI CASTG 1 cut(s) 301
TseI GCWGC 2 cut(s) 11, 249
TspDTI ATGAA 2 cut(s) 63, 204
TspRI CASTG 1 cut(s) 301
XagI CCTNNNNNAGG 2 cut(s) 36, 179
ZrmI AGTACT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.