RchiOBHm_Chr1g0353691

threonine aspartase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0004496
Physical Location & Seq
Forward (+)
46872609 .. 46876623
4015 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1119 bp
ATGTTTGGTCTTGTTTTGCTTCAGGGCTCTGGTAGGTGCATAGATGCTGTCACTGCAGCTATTCAAGTGTTGGAGGATGATCCAATCACGAACGCAGGCCGGGGGTCTAATTTGACTGAAGATGGTCGTGTAGAATGTGATGCCAGTATTATGGACGGGAATTCTGGAGTATTTGGAGCTGTTGGAGCATTGCCAGGTGTCAAAAACCCCATCAAGGTTGCTGCTTTACTGGCCAAGGAGCAAATGATGGGATCCTCCCAGTTGGGCCGAATACCTCCCATCTTCCTCGTTGGTGAAGGTGCCCGCATATGGGCGAAGTCCAAGGGCATTGCTTTGTCGGAAAGCACATCAGCTGCTGATGAGTGGCTGGTAACGGAAAGGGCAAAAACACATTGGAAAAAGTACAAAGCAATGGTAGATGATGCAAAAGCAAAGACAGAAACTTCTGCTGGTGGACTTCCTTGTAGTCCTCAAGAGGCTGCTGGAATTCCAGAATTAGAAGCTCAACCATGTGATTTTTTGAATAAAAATGGGGCAGAGGAGGATTGCATCATGGACACTGTAGGAGTTATTTGTGTAGATGCTGACGGACAAATTGCATCTGGTGCCTCAAGTGGAGGCATTGCATTAAAGGTTGGTGGGCGTGTAGGGTTAGCAGCAATGCATGGTTCAGGCTGTTGGGTATCCTCCAAAGGTCTTTTTGGGGCTCCATGTATAGTCGGTTGTTGTGTAAGTGGTGCTGGAGAATATCTGATGAAAGGGTTTGCAGCTCGAGAGTGCTGTGTCTCTTTATCACTTTCACAGGCAGGTCCTGCCTCTGCTTGCATGAAAGTTTTACGCTCCTTTGTTCAAGAGAGTAATCAAAATTGTACTGATAGAAGTGCCGGATTTCTAGCTGTGCAAGCTGATGCTCCTGTGATGGTTTCAGGAAGTTCTCCAAAGTTAAAAGCTATTGAGATTGCAGCTGCTTACTCTTCCTTATCCTTTGGGATAGGATTTTTTGGAAGCTCTATGGAGCGACCCAAGGTTTCAATTCTCAGGAGTACAAAATTGCAAACCAAAACTGGAGTAGACCATTTTGAAGCACGAATTGATCTTTCTCCTAAAAGGAAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

372

Amino Acids

38.49

Weight (kDa)

6.36

Isoelectric Point (pI)

34.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 5 - 303 3.3e-44 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 797
AccB1I GGYRCC 2 cut(s) 299, 605
AccI GTMKAC 1 cut(s) 1071
AciI CCGC 1 cut(s) 304
AclWI GGATC 3 cut(s) 74, 246, 259
AcoI YGGCCR 1 cut(s) 231
AcsI RAATTY 2 cut(s) 160, 486
AcuI CTGAAG 2 cut(s) 5, 138
AfaI GTAC 3 cut(s) 404, 871, 1045
AfiI CCNNNNNNNGG 3 cut(s) 214, 309, 310
AgsI TTSAA 5 cut(s) 65, 523, 851, 1032, 1082
AjnI CCWGG 1 cut(s) 193
Alw26I GTCTC 1 cut(s) 790
AlwI GGATC 3 cut(s) 74, 246, 259
AlwNI CAGNNNCTG 2 cut(s) 356, 812
Ama87I CYCGRG 1 cut(s) 771
AoxI GGCC 3 cut(s) 97, 231, 265
ApeKI GCWGC 8 cut(s) 56, 221, 353, 479, 656, 767, 962, 965
ApoI RAATTY 2 cut(s) 160, 486
AspS9I GGNCC 2 cut(s) 265, 809
AsuC2I CCSGG 1 cut(s) 101
AsuHPI GGTGA 1 cut(s) 305
AvaI CYCGRG 1 cut(s) 771
AvaII GGWCC 1 cut(s) 809
BaeGI GKGCMC 1 cut(s) 304
BalI TGGCCA 1 cut(s) 233
BamHI GGATCC 1 cut(s) 251
BanI GGYRCC 2 cut(s) 299, 605
BanII GRGCYC 2 cut(s) 29, 709
BbvI GCAGC 8 cut(s) 68, 208, 340, 466, 668, 779, 952, 974
BccI CCATC 5 cut(s) 116, 218, 241, 287, 913
BciT130I CCWGG 1 cut(s) 195
BciVI GTATCC 1 cut(s) 694
BcnI CCSGG 1 cut(s) 101
BcoDI GTCTC 1 cut(s) 790
BfaI CTAG 1 cut(s) 893
BfmI CTRYAG 2 cut(s) 54, 561
BfuAI ACCTGC 1 cut(s) 797
BfuI GTATCC 1 cut(s) 694
BisI GCNGC 8 cut(s) 57, 222, 354, 480, 657, 768, 963, 966
BlsI GCNGC 8 cut(s) 58, 223, 355, 481, 658, 769, 964, 967
Bme1390I CCNGG 2 cut(s) 101, 195
Bme18I GGWCC 1 cut(s) 809
BmeT110I CYCGRG 1 cut(s) 771
BmgT120I GGNCC 2 cut(s) 265, 809
BmiI GGNNCC 4 cut(s) 253, 301, 607, 708
BmrFI CCNGG 2 cut(s) 101, 195
BmrI ACTGGG 1 cut(s) 253
BmsI GCATC 7 cut(s) 34, 130, 412, 558, 571, 608, 898
BmuI ACTGGG 1 cut(s) 253
BpmI CTGGAG 3 cut(s) 186, 762, 1086
BpuEI CTTGAG 2 cut(s) 456, 595
BpuMI CCSGG 1 cut(s) 101
BsaJI CCNNGG 4 cut(s) 100, 234, 321, 1023
Bsc4I CCNNNNNNNGG 3 cut(s) 214, 309, 310
Bse1I ACTGG 4 cut(s) 144, 234, 259, 1069
Bse3DI GCAATG 5 cut(s) 188, 327, 417, 621, 666
BseBI CCWGG 1 cut(s) 195
BseDI CCNNGG 4 cut(s) 100, 234, 321, 1023
BseGI GGATG 1 cut(s) 82
BseLI CCNNNNNNNGG 3 cut(s) 214, 309, 310
BseMI GCAATG 5 cut(s) 188, 327, 417, 621, 666
BseMII CTCAG 1 cut(s) 1051
BseNI ACTGG 4 cut(s) 144, 234, 259, 1069
BseRI GAGGAG 1 cut(s) 554
BseSI GKGCMC 1 cut(s) 304
BseXI GCAGC 8 cut(s) 68, 208, 340, 466, 668, 779, 952, 974
BshFI GGCC 3 cut(s) 99, 233, 267
BshNI GGYRCC 2 cut(s) 299, 605
BsiHKCI CYCGRG 1 cut(s) 771
BsiSI CCGG 2 cut(s) 100, 885
BslI CCNNNNNNNGG 3 cut(s) 214, 309, 310
BsmAI GTCTC 1 cut(s) 790
BsnI GGCC 3 cut(s) 99, 233, 267
BsoBI CYCGRG 1 cut(s) 771
Bsp1286I GDGCHC 3 cut(s) 29, 304, 709
Bsp143I GATC 3 cut(s) 79, 251, 1093
BspACI CCGC 1 cut(s) 304
BspANI GGCC 3 cut(s) 99, 233, 267
BspCNI CTCAG 1 cut(s) 1050
BspLI GGNNCC 4 cut(s) 253, 301, 607, 708
BspMAI CTGCAG 1 cut(s) 58
BspMI ACCTGC 1 cut(s) 797
BspPI GGATC 3 cut(s) 74, 246, 259
BspT107I GGYRCC 2 cut(s) 299, 605
BsrDI GCAATG 5 cut(s) 188, 327, 417, 621, 666
BsrI ACTGG 4 cut(s) 144, 234, 259, 1069
BssECI CCNNGG 4 cut(s) 100, 234, 321, 1023
BssMI GATC 3 cut(s) 79, 251, 1093
BssT1I CCWWGG 3 cut(s) 234, 321, 1023
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 1 cut(s) 562
Bst6I CTCTTC 1 cut(s) 979
BstAPI GCANNNNNTGC 2 cut(s) 605, 812
BstC8I GCNNGC 4 cut(s) 97, 304, 823, 903
BstDEI CTNAG 1 cut(s) 1037
BstF5I GGATG 1 cut(s) 82
BstKTI GATC 3 cut(s) 82, 254, 1096
BstMAI GTCTC 1 cut(s) 790
BstMBI GATC 3 cut(s) 79, 251, 1093
BstMWI GCNNNNNNNGC 6 cut(s) 53, 185, 230, 605, 812, 902
BstNI CCWGG 1 cut(s) 195
BstSCI CCNGG 2 cut(s) 99, 193
BstSFI CTRYAG 2 cut(s) 54, 561
BstSLI GKGCMC 1 cut(s) 304
BstV1I GCAGC 8 cut(s) 68, 208, 340, 466, 668, 779, 952, 974
BstX2I RGATCY 1 cut(s) 251
BstXI CCANNNNNNTGG 1 cut(s) 151
BstYI RGATCY 1 cut(s) 251
BsuI GTATCC 1 cut(s) 694
BsuRI GGCC 3 cut(s) 99, 233, 267
BtsCI GGATG 1 cut(s) 82
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 2 cut(s) 51, 558
BveI ACCTGC 1 cut(s) 797
Cac8I GCNNGC 4 cut(s) 97, 304, 823, 903
CaiI CAGNNNCTG 2 cut(s) 356, 812
Cfr13I GGNCC 2 cut(s) 265, 809
Csp6I GTAC 3 cut(s) 403, 870, 1044
CviAII CATG 5 cut(s) 510, 553, 665, 711, 826
CviQI GTAC 3 cut(s) 403, 870, 1044
DdeI CTNAG 1 cut(s) 1037
DpnI GATC 3 cut(s) 81, 253, 1095
DpnII GATC 3 cut(s) 79, 251, 1093
EaeI YGGCCR 1 cut(s) 231
Eam1104I CTCTTC 1 cut(s) 979
EarI CTCTTC 1 cut(s) 979
Eco130I CCWWGG 3 cut(s) 234, 321, 1023
Eco24I GRGCYC 2 cut(s) 29, 709
Eco47I GGWCC 1 cut(s) 809
Eco57I CTGAAG 2 cut(s) 5, 138
Eco88I CYCGRG 1 cut(s) 771
EcoO109I RGGNCCY 1 cut(s) 809
EcoRI GAATTC 2 cut(s) 160, 486
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 3 cut(s) 234, 321, 1023
EcoT22I ATGCAT 1 cut(s) 666
EcoT38I GRGCYC 2 cut(s) 29, 709
ErhI CCWWGG 3 cut(s) 234, 321, 1023
FaeI CATG 5 cut(s) 513, 556, 668, 714, 829
FatI CATG 5 cut(s) 509, 552, 664, 710, 825
FauI CCCGC 1 cut(s) 311
FauNDI CATATG 1 cut(s) 308
FblI GTMKAC 1 cut(s) 1071
Fnu4HI GCNGC 8 cut(s) 57, 222, 354, 480, 657, 768, 963, 966
FokI GGATG 1 cut(s) 89
FriOI GRGCYC 2 cut(s) 29, 709
Fsp4HI GCNGC 8 cut(s) 57, 222, 354, 480, 657, 768, 963, 966
FspBI CTAG 1 cut(s) 893
GluI GCNGC 8 cut(s) 57, 222, 354, 480, 657, 768, 963, 966
GsuI CTGGAG 3 cut(s) 186, 762, 1086
HaeIII GGCC 3 cut(s) 99, 233, 267
HapII CCGG 2 cut(s) 100, 885
Hin1II CATG 5 cut(s) 513, 556, 668, 714, 829
HpaII CCGG 2 cut(s) 100, 885
HphI GGTGA 1 cut(s) 305
Hpy166II GTNNAC 2 cut(s) 455, 1072
Hpy188I TCNGA 2 cut(s) 340, 753
Hpy188III TCNNGA 8 cut(s) 88, 165, 473, 491, 773, 851, 927, 1039
Hpy8I GTNNAC 2 cut(s) 455, 1072
HpyAV CCTTC 1 cut(s) 290
HpyCH4III ACNGT 1 cut(s) 562
HpyF10VI GCNNNNNNNGC 6 cut(s) 53, 185, 230, 605, 812, 902
HpyF3I CTNAG 1 cut(s) 1037
Hsp92II CATG 5 cut(s) 513, 556, 668, 714, 829
Kzo9I GATC 3 cut(s) 79, 251, 1093
LmnI GCTCC 7 cut(s) 176, 185, 238, 712, 845, 916, 1015
Lsp1109I GCAGC 8 cut(s) 68, 208, 340, 466, 668, 779, 952, 974
LweI GCATC 7 cut(s) 34, 130, 412, 558, 571, 608, 898
MaeI CTAG 1 cut(s) 893
MaeIII GTNAC 2 cut(s) 49, 370
MalI GATC 3 cut(s) 81, 253, 1095
MboI GATC 3 cut(s) 79, 251, 1093
MboII GAAGA 3 cut(s) 131, 274, 966
MflI RGATCY 1 cut(s) 251
MhlI GDGCHC 3 cut(s) 29, 304, 709
MlsI TGGCCA 1 cut(s) 233
MluCI AATT 9 cut(s) 109, 160, 486, 494, 594, 865, 1032, 1049, 1089
MluNI TGGCCA 1 cut(s) 233
MmeI TCCRAC 3 cut(s) 51, 163, 318
Mox20I TGGCCA 1 cut(s) 233
Mph1103I ATGCAT 1 cut(s) 666
MscI TGGCCA 1 cut(s) 233
MseI TTAA 2 cut(s) 629, 944
Msp20I TGGCCA 1 cut(s) 233
MspA1I CMGCKG 2 cut(s) 353, 965
MspI CCGG 2 cut(s) 100, 885
MspR9I CCNGG 2 cut(s) 101, 195
MvaI CCWGG 1 cut(s) 195
MwoI GCNNNNNNNGC 6 cut(s) 53, 185, 230, 605, 812, 902
NciI CCSGG 1 cut(s) 101
NdeI CATATG 1 cut(s) 308
NdeII GATC 3 cut(s) 79, 251, 1093
NlaIII CATG 5 cut(s) 513, 556, 668, 714, 829
NlaIV GGNNCC 4 cut(s) 253, 301, 607, 708
NmuCI GTSAC 1 cut(s) 49
NsiI ATGCAT 1 cut(s) 666
PaeR7I CTCGAG 1 cut(s) 771
PkrI GCNGC 8 cut(s) 58, 223, 355, 481, 658, 769, 964, 967
PpuMI RGGWCCY 1 cut(s) 809
Psp5II RGGWCCY 1 cut(s) 809
Psp6I CCWGG 1 cut(s) 193
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 4 cut(s) 253, 301, 607, 708
PspPI GGNCC 2 cut(s) 265, 809
PspPPI RGGWCCY 1 cut(s) 809
PstI CTGCAG 1 cut(s) 58
PstNI CAGNNNCTG 2 cut(s) 356, 812
PsuI RGATCY 1 cut(s) 251
PvuII CAGCTG 2 cut(s) 353, 965
RsaI GTAC 3 cut(s) 404, 871, 1045
RsaNI GTAC 3 cut(s) 403, 870, 1044
SaqAI TTAA 2 cut(s) 629, 944
SatI GCNGC 8 cut(s) 57, 222, 354, 480, 657, 768, 963, 966
Sau3AI GATC 3 cut(s) 79, 251, 1093
Sau96I GGNCC 2 cut(s) 265, 809
ScrFI CCNGG 2 cut(s) 101, 195
SduI GDGCHC 3 cut(s) 29, 304, 709
SfaNI GCATC 7 cut(s) 34, 130, 412, 558, 571, 608, 898
SfcI CTRYAG 2 cut(s) 54, 561
Sfr274I CTCGAG 1 cut(s) 771
SinI GGWCC 1 cut(s) 809
SlaI CTCGAG 1 cut(s) 771
SmlI CTYRAG 3 cut(s) 471, 610, 771
SmoI CTYRAG 3 cut(s) 471, 610, 771
Sse9I AATT 9 cut(s) 109, 160, 486, 494, 594, 865, 1032, 1049, 1089
SsiI CCGC 1 cut(s) 304
SspMI CTAG 1 cut(s) 893
StyD4I CCNGG 2 cut(s) 99, 193
StyI CCWWGG 3 cut(s) 234, 321, 1023
TaaI ACNGT 1 cut(s) 562
TaqI TCGA 1 cut(s) 772
TasI AATT 9 cut(s) 109, 160, 486, 494, 594, 865, 1032, 1049, 1089
TatI WGTACW 3 cut(s) 402, 869, 1043
Tru1I TTAA 2 cut(s) 629, 944
Tru9I TTAA 2 cut(s) 629, 944
TscAI CASTG 2 cut(s) 58, 565
TseFI GTSAC 1 cut(s) 49
TseI GCWGC 8 cut(s) 56, 221, 353, 479, 656, 767, 962, 965
Tsp45I GTSAC 1 cut(s) 49
TspDTI ATGAA 2 cut(s) 770, 842
TspGWI ACGGA 2 cut(s) 389, 603
TspRI CASTG 2 cut(s) 58, 565
VpaK11BI GGWCC 1 cut(s) 809
XapI RAATTY 2 cut(s) 160, 486
XhoI CTCGAG 1 cut(s) 771
XmiI GTMKAC 1 cut(s) 1071
XspI CTAG 1 cut(s) 893
Zsp2I ATGCAT 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.