RchiOBHm_Chr1g0374701

21 kDa protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005396
Physical Location & Seq
Forward (+)
62439267 .. 62439868
602 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 576 bp
ATGGGAACTCCCACATTTCTCTCCACTCTTCTCCTATTCATCCTCTTCATCAACACGACCACGGCTACTTCTTCTTCGAGCCAAACCTACAAAACCTACGTCAAAACAGCCTGCAACTCCACCACGTACCCAGAAATTTGCTACAAGGCTCTCTCCACCTACGCCAGATCCGTCAAATCAAACCCTCAGAAGCTCTGCAACTATGCCCTCTCCGTAACCCTAAAAGCCGCGAAAAAAGCCTCTTCCACGGTGTCGAAGCTGTCCAATAAGAAAGGATTGACGAAAGCAGAGGCCGGGGTAGTGGAGGATTGCATAGAGAACATTGAGGATTCCGTCGATGAGCTCAAACAGGCGGTGACTGCCATGAAAAATTTGGGAGATGCGGATGTCCAACTTCATTTGGATGACATAAAGACTTGGGTTAGCGCTTCGCTGACGGATGAGGACACCTGTACGGATGGATTCGATGAGCAGAAGGTAAGTGCGGCGGTGAAGAACACCATTAATAAGAGCATTCTCACTGTTGCTAGATTGGCTAGCAATGCTTTATCTCTCATCAACAACCTCAGTTACTAG

Protein Analysis

191

Amino Acids

20.63

Weight (kDa)

7.55

Isoelectric Point (pI)

37.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 33 - 185 1.3e-37 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 230
AciI CCGC 5 cut(s) 228, 353, 383, 485, 488
AclWI GGATC 1 cut(s) 162
AcsI RAATTY 2 cut(s) 135, 370
AfaI GTAC 2 cut(s) 128, 454
AfeI AGCGCT 1 cut(s) 427
AluBI AGCT 3 cut(s) 193, 259, 343
AluI AGCT 3 cut(s) 193, 259, 343
Alw21I GWGCWC 1 cut(s) 345
AlwI GGATC 1 cut(s) 162
Aor51HI AGCGCT 1 cut(s) 427
AoxI GGCC 1 cut(s) 291
ApoI RAATTY 2 cut(s) 135, 370
ArsI GACNNNNNNTTYG 2 cut(s) 84, 116
AseI ATTAAT 1 cut(s) 504
AspLEI GCGC 1 cut(s) 428
AsuC2I CCSGG 1 cut(s) 295
AsuHPI GGTGA 2 cut(s) 367, 502
AsuNHI GCTAGC 1 cut(s) 536
BanII GRGCYC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 345
BccI CCATC 1 cut(s) 452
BceAI ACGGC 1 cut(s) 78
BcnI CCSGG 1 cut(s) 295
BfaI CTAG 3 cut(s) 528, 537, 574
BfoI RGCGCY 1 cut(s) 429
BisI GCNGC 2 cut(s) 228, 486
BlsI GCNGC 2 cut(s) 229, 487
Bme1390I CCNGG 1 cut(s) 295
BmrFI CCNGG 1 cut(s) 295
BmsI GCATC 1 cut(s) 370
BmtI GCTAGC 1 cut(s) 540
BpuMI CCSGG 1 cut(s) 295
BsaAI YACGTR 1 cut(s) 126
BsaJI CCNNGG 3 cut(s) 60, 246, 294
Bse3DI GCAATG 1 cut(s) 547
BseDI CCNNGG 3 cut(s) 60, 246, 294
BseGI GGATG 5 cut(s) 39, 391, 409, 445, 463
BseMI GCAATG 1 cut(s) 547
BseMII CTCAG 1 cut(s) 200
Bsh1236I CGCG 1 cut(s) 230
BshFI GGCC 1 cut(s) 293
BsiHKAI GWGCWC 1 cut(s) 345
BsiSI CCGG 1 cut(s) 294
BsmI GAATGC 1 cut(s) 513
BsnI GGCC 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 345
Bsp143I GATC 1 cut(s) 167
BspACI CCGC 5 cut(s) 228, 353, 383, 485, 488
BspANI GGCC 1 cut(s) 293
BspCNI CTCAG 1 cut(s) 199
BspFNI CGCG 1 cut(s) 230
BspOI GCTAGC 1 cut(s) 540
BspPI GGATC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 547
BssECI CCNNGG 3 cut(s) 60, 246, 294
BssMI GATC 1 cut(s) 167
Bst4CI ACNGT 2 cut(s) 250, 523
Bst6I CTCTTC 3 cut(s) 33, 50, 247
BstBAI YACGTR 1 cut(s) 126
BstC8I GCNNGC 2 cut(s) 112, 538
BstDEI CTNAG 2 cut(s) 186, 566
BstDSI CCRYGG 2 cut(s) 60, 246
BstF5I GGATG 5 cut(s) 39, 391, 409, 445, 463
BstFNI CGCG 1 cut(s) 230
BstH2I RGCGCY 1 cut(s) 429
BstHHI GCGC 1 cut(s) 428
BstKTI GATC 1 cut(s) 170
BstMBI GATC 1 cut(s) 167
BstMWI GCNNNNNNNGC 4 cut(s) 236, 359, 533, 542
BstSCI CCNGG 1 cut(s) 293
BstUI CGCG 1 cut(s) 230
BstX2I RGATCY 1 cut(s) 167
BstYI RGATCY 1 cut(s) 167
BsuRI GGCC 1 cut(s) 293
BtgI CCRYGG 2 cut(s) 60, 246
BtsCI GGATG 5 cut(s) 39, 391, 409, 445, 463
BtsIMutI CAGTG 1 cut(s) 519
Cac8I GCNNGC 2 cut(s) 112, 538
CfoI GCGC 1 cut(s) 428
Csp6I GTAC 2 cut(s) 127, 453
CviAII CATG 1 cut(s) 364
CviQI GTAC 2 cut(s) 127, 453
DdeI CTNAG 2 cut(s) 186, 566
DpnI GATC 1 cut(s) 169
DpnII GATC 1 cut(s) 167
Eam1104I CTCTTC 3 cut(s) 33, 50, 247
EarI CTCTTC 3 cut(s) 33, 50, 247
Ecl136II GAGCTC 1 cut(s) 343
Eco24I GRGCYC 1 cut(s) 345
Eco47III AGCGCT 1 cut(s) 427
Eco53kI GAGCTC 1 cut(s) 343
EcoICRI GAGCTC 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 345
FaeI CATG 1 cut(s) 367
FaiI YATR 4 cut(s) 204, 314, 365, 410
FatI CATG 1 cut(s) 363
Fnu4HI GCNGC 2 cut(s) 228, 486
FokI GGATG 5 cut(s) 26, 398, 416, 452, 470
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 2 cut(s) 228, 486
FspBI CTAG 3 cut(s) 528, 537, 574
GlaI GCGC 1 cut(s) 427
GluI GCNGC 2 cut(s) 228, 486
HaeII RGCGCY 1 cut(s) 429
HaeIII GGCC 1 cut(s) 293
HapII CCGG 1 cut(s) 294
HhaI GCGC 1 cut(s) 428
Hin1II CATG 1 cut(s) 367
Hin6I GCGC 1 cut(s) 426
HinP1I GCGC 1 cut(s) 426
HinfI GANTC 2 cut(s) 329, 462
HpaII CCGG 1 cut(s) 294
HphI GGTGA 2 cut(s) 367, 502
Hpy188I TCNGA 1 cut(s) 189
Hpy99I CGWCG 1 cut(s) 338
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 2 cut(s) 250, 523
HpyCH4IV ACGT 2 cut(s) 99, 125
HpyCH4V TGCA 3 cut(s) 114, 198, 312
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 359, 533, 542
HpyF3I CTNAG 2 cut(s) 186, 566
HpySE526I ACGT 2 cut(s) 99, 125
Hsp92II CATG 1 cut(s) 367
HspAI GCGC 1 cut(s) 426
Kzo9I GATC 1 cut(s) 167
LpnPI CCDG 6 cut(s) 124, 144, 178, 307, 335, 463
LweI GCATC 1 cut(s) 370
MaeI CTAG 3 cut(s) 528, 537, 574
MaeII ACGT 2 cut(s) 99, 125
MaeIII GTNAC 3 cut(s) 214, 355, 569
MalI GATC 1 cut(s) 169
MboI GATC 1 cut(s) 167
MboII GAAGA 6 cut(s) 20, 37, 63, 66, 234, 505
MflI RGATCY 1 cut(s) 167
MhlI GDGCHC 1 cut(s) 345
MluCI AATT 2 cut(s) 135, 370
MmeI TCCRAC 1 cut(s) 415
MnlI CCTC 9 cut(s) 53, 195, 218, 250, 283, 298, 319, 436, 575
MseI TTAA 1 cut(s) 504
MslI CAYNNNNRTG 1 cut(s) 402
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 1 cut(s) 295
Mva1269I GAATGC 1 cut(s) 513
MvnI CGCG 1 cut(s) 230
MwoI GCNNNNNNNGC 4 cut(s) 236, 359, 533, 542
NciI CCSGG 1 cut(s) 295
NdeII GATC 1 cut(s) 167
NheI GCTAGC 1 cut(s) 536
NlaIII CATG 1 cut(s) 367
NmuCI GTSAC 1 cut(s) 355
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PctI GAATGC 1 cut(s) 513
PfeI GAWTC 2 cut(s) 329, 462
PkrI GCNGC 2 cut(s) 229, 487
Ppu21I YACGTR 1 cut(s) 126
PshBI ATTAAT 1 cut(s) 504
Psp124BI GAGCTC 1 cut(s) 345
PsuI RGATCY 1 cut(s) 167
RsaI GTAC 2 cut(s) 128, 454
RsaNI GTAC 2 cut(s) 127, 453
RseI CAYNNNNRTG 1 cut(s) 402
SacI GAGCTC 1 cut(s) 345
SaqAI TTAA 1 cut(s) 504
SatI GCNGC 2 cut(s) 228, 486
Sau3AI GATC 1 cut(s) 167
ScrFI CCNGG 1 cut(s) 295
SduI GDGCHC 1 cut(s) 345
SfaNI GCATC 1 cut(s) 370
SmiMI CAYNNNNRTG 1 cut(s) 402
Sse9I AATT 2 cut(s) 135, 370
SsiI CCGC 5 cut(s) 228, 353, 383, 485, 488
SspMI CTAG 3 cut(s) 528, 537, 574
SstI GAGCTC 1 cut(s) 345
StyD4I CCNGG 1 cut(s) 293
TaaI ACNGT 2 cut(s) 250, 523
TaiI ACGT 2 cut(s) 102, 128
TaqI TCGA 4 cut(s) 77, 254, 336, 465
TasI AATT 2 cut(s) 135, 370
TauI GCSGC 2 cut(s) 230, 488
TfiI GAWTC 2 cut(s) 329, 462
Tru1I TTAA 1 cut(s) 504
Tru9I TTAA 1 cut(s) 504
TscAI CASTG 1 cut(s) 526
TseFI GTSAC 1 cut(s) 355
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 4 cut(s) 28, 37, 380, 386
TspGWI ACGGA 5 cut(s) 160, 202, 322, 452, 470
TspRI CASTG 1 cut(s) 526
VspI ATTAAT 1 cut(s) 504
XapI RAATTY 2 cut(s) 135, 370
XcmI CCANNNNNNNNNTGG 1 cut(s) 370
XspI CTAG 3 cut(s) 528, 537, 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.