RchiOBHm_Chr1g0382961
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-independent O- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0003088
Physical Location & Seq
Reverse (-)
67526654 .. 67529478
2825 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1098 bp
ATGGGTTCAACCGGCGAGACTCAGATGACTCCGACCCAAGTCTCCGACGAGGAAGCCAACCTCTTTGCCATGCAACTCGCCAGCGCCTCCGTCCTCCCCATGGTTCTCAAAGCCGCCATTGAGCTCGACCTCTTGGAGATCATGGCCAAGGCCGGACCCGGCGCGTTCCTCTCCCCTAATGACCTAGCCTCTCAGCTTCCGACCAAGAACCCCGAAGCTCCGGTCATGCTTGACCGGATGCTTCGCCTTCTGGCCAGCTACTCCATTCTAACCTACTCCTTGCGTACACTTCCGGACGGTAAAGTTGAGAGGCTCTACGGTTTGGGACCTGTGTGTAAATTCTTGACCAAGAACGAAGATGGTGTCTCCATTGCTGCTCTCTGCCTCATGAACCAAGACAAGGTCCTCGTCGAGAGCTGGTATCATCTAAAGGATGCAGTTCTTGATGGTGGGATTCCATTTAACAAGGCCTATGGAATGACTGCTTTTGATTACCATGGAACTGACCCTAGATTCAACAAGGTCTTCAACAAGGGAATGGCTGACCACTCCACCATTACCATGAAGAAAATCCTTGAGACTTACAAAGGCTTTGAGGGCCTCACATCCATCGTTGATGTCGGAGGCGGCACCGGAGCTGTTGTTAACATGATCGTTTCTAAGTACCCTTCGATCAAGGGCATCAACTTTGACTTGCCTCATGTGATCGAAGATGCTCCTCAATATCCTGGTGTGCAACATGTTGGAGGGGACATGTTTGTAAGTGTACCGAAAGGAGATGCAATTTTCATGAAGTGGATATGTCACGACTGGAGTGACGAGCACTGCTTGAAATTCTTGAAGAACTGCTATGCAGCGCTTCCAGACAATGGGAAAGTGATTCTTGGTGAGTGCATTCTGCCTGTAGCACCGGACACTAGCCTCGCCACCAAGGGAGTTGTCCATATCGACGTGATCATGTTGGCTCACAACCCCGGTGGCAAAGAGAGGACGGAGCAGGAGTTTGAAGCCCTGGCTAAGGGGTCTGGATTTCAAGGCATTCGAGTAGCATGTAATGCTTTCAACACCTATGTCATCGAGTTTCTTAAGAAGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
Dimerisation Methyltransf_2

Protein Analysis

365

Amino Acids

39.74

Weight (kDa)

5.62

Isoelectric Point (pI)

30.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 24 - 117 2.1e-17 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 140 - 345 7.5e-80 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 629
AccB7I CCANNNNNTGG 1 cut(s) 869
AccII CGCG 1 cut(s) 164
AccIII TCCGGA 1 cut(s) 292
AciI CCGC 2 cut(s) 114, 627
AcoI YGGCCR 2 cut(s) 144, 252
AcsI RAATTY 2 cut(s) 338, 833
AfaI GTAC 3 cut(s) 286, 665, 768
AfeI AGCGCT 1 cut(s) 858
AfiI CCNNNNNNNGG 4 cut(s) 100, 400, 869, 1018
AflII CTTAAG 1 cut(s) 1085
AflIII ACRYGT 2 cut(s) 739, 753
AgsI TTSAA 8 cut(s) 9, 517, 529, 832, 841, 1007, 1034, 1063
AjiI CACGTC 1 cut(s) 952
AjnI CCWGG 2 cut(s) 727, 1011
AluBI AGCT 6 cut(s) 124, 196, 218, 258, 417, 638
AluI AGCT 6 cut(s) 124, 196, 218, 258, 417, 638
Alw21I GWGCWC 2 cut(s) 126, 825
Alw26I GTCTC 4 cut(s) 11, 46, 370, 572
Aor13HI TCCGGA 1 cut(s) 292
Aor51HI AGCGCT 1 cut(s) 858
AoxI GGCC 5 cut(s) 144, 150, 252, 468, 598
ApeKI GCWGC 2 cut(s) 374, 854
ApoI RAATTY 2 cut(s) 338, 833
ArsI GACNNNNNNTTYG 4 cut(s) 207, 239, 348, 380
AspLEI GCGC 3 cut(s) 86, 164, 859
AspS9I GGNCC 4 cut(s) 155, 326, 403, 598
AsuC2I CCSGG 2 cut(s) 159, 975
AsuHPI GGTGA 1 cut(s) 899
AvaII GGWCC 3 cut(s) 155, 326, 403
BalI TGGCCA 2 cut(s) 146, 254
BanI GGYRCC 1 cut(s) 629
BanII GRGCYC 1 cut(s) 126
BbsI GAAGAC 1 cut(s) 517
Bbv12I GWGCWC 2 cut(s) 126, 825
BbvI GCAGC 2 cut(s) 361, 866
BccI CCATC 3 cut(s) 353, 440, 617
BciT130I CCWGG 2 cut(s) 729, 1013
BclI TGATCA 1 cut(s) 954
BcnI CCSGG 2 cut(s) 159, 975
BcoDI GTCTC 4 cut(s) 11, 46, 370, 572
BfaI CTAG 3 cut(s) 185, 510, 918
BfmI CTRYAG 1 cut(s) 903
BfoI RGCGCY 2 cut(s) 87, 860
BfrI CTTAAG 1 cut(s) 1085
BglII AGATCT 1 cut(s) 1092
BisI GCNGC 4 cut(s) 114, 375, 628, 855
BlsI GCNGC 4 cut(s) 115, 376, 629, 856
Bme1390I CCNGG 4 cut(s) 159, 729, 975, 1013
Bme18I GGWCC 3 cut(s) 155, 326, 403
BmgBI CACGTC 1 cut(s) 952
BmgT120I GGNCC 4 cut(s) 155, 326, 403, 598
BmiI GGNNCC 3 cut(s) 157, 327, 631
BmrFI CCNGG 4 cut(s) 159, 729, 975, 1013
BmsI GCATC 5 cut(s) 228, 424, 690, 703, 769
BoxI GACNNNNGTC 1 cut(s) 38
BpiI GAAGAC 1 cut(s) 517
BpmI CTGGAG 1 cut(s) 832
Bpu10I CCTNAGC 1 cut(s) 1017
BpuEI CTTGAG 1 cut(s) 596
BpuMI CCSGG 2 cut(s) 159, 975
BsaJI CCNNGG 6 cut(s) 99, 147, 496, 930, 973, 1011
BsaWI WCCGGW 5 cut(s) 220, 234, 292, 632, 910
BsaXI ACNNNNNCTCC 4 cut(s) 627, 657, 986, 1016
Bsc4I CCNNNNNNNGG 4 cut(s) 100, 400, 869, 1018
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 1 cut(s) 815
Bse3DI GCAATG 1 cut(s) 369
BseAI TCCGGA 1 cut(s) 292
BseBI CCWGG 2 cut(s) 729, 1013
BseDI CCNNGG 6 cut(s) 99, 147, 496, 930, 973, 1011
BseGI GGATG 3 cut(s) 243, 439, 605
BseLI CCNNNNNNNGG 4 cut(s) 100, 400, 869, 1018
BseMI GCAATG 1 cut(s) 369
BseMII CTCAG 2 cut(s) 35, 206
BseNI ACTGG 1 cut(s) 815
BseRI GAGGAG 1 cut(s) 708
BseXI GCAGC 2 cut(s) 361, 866
Bsh1236I CGCG 1 cut(s) 164
BshFI GGCC 5 cut(s) 146, 152, 254, 470, 600
BshNI GGYRCC 1 cut(s) 629
BsiHKAI GWGCWC 2 cut(s) 126, 825
BsiSI CCGG 9 cut(s) 12, 153, 159, 221, 235, 293, 633, 911, 975
BslFI GGGAC 2 cut(s) 339, 764
BslI CCNNNNNNNGG 4 cut(s) 100, 400, 869, 1018
BsmAI GTCTC 4 cut(s) 11, 46, 370, 572
BsmFI GGGAC 2 cut(s) 339, 764
BsmI GAATGC 2 cut(s) 894, 1038
BsnI GGCC 5 cut(s) 146, 152, 254, 470, 600
Bsp1286I GDGCHC 2 cut(s) 126, 825
Bsp13I TCCGGA 1 cut(s) 292
Bsp143I GATC 6 cut(s) 138, 651, 672, 705, 954, 1092
Bsp19I CCATGG 2 cut(s) 99, 496
BspACI CCGC 2 cut(s) 114, 627
BspANI GGCC 5 cut(s) 146, 152, 254, 470, 600
BspCNI CTCAG 2 cut(s) 34, 205
BspEI TCCGGA 1 cut(s) 292
BspFNI CGCG 1 cut(s) 164
BspHI TCATGA 2 cut(s) 387, 789
BspLI GGNNCC 3 cut(s) 157, 327, 631
BspT107I GGYRCC 1 cut(s) 629
BspTI CTTAAG 1 cut(s) 1085
BsrDI GCAATG 1 cut(s) 369
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 1 cut(s) 815
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 6 cut(s) 99, 147, 496, 930, 973, 1011
BssMI GATC 6 cut(s) 138, 651, 672, 705, 954, 1092
BssT1I CCWWGG 4 cut(s) 99, 147, 496, 930
Bst2UI CCWGG 2 cut(s) 729, 1013
Bst4CI ACNGT 2 cut(s) 299, 320
BstAFI CTTAAG 1 cut(s) 1085
BstAPI GCANNNNNTGC 1 cut(s) 1055
BstC8I GCNNGC 2 cut(s) 82, 256
BstDEI CTNAG 4 cut(s) 21, 192, 660, 1017
BstDSI CCRYGG 2 cut(s) 99, 496
BstENI CCTNNNNNAGG 1 cut(s) 1016
BstF5I GGATG 3 cut(s) 243, 439, 605
BstFNI CGCG 1 cut(s) 164
BstH2I RGCGCY 2 cut(s) 87, 860
BstHHI GCGC 3 cut(s) 86, 164, 859
BstKTI GATC 6 cut(s) 141, 654, 675, 708, 957, 1095
BstMAI GTCTC 4 cut(s) 11, 46, 370, 572
BstMBI GATC 6 cut(s) 138, 651, 672, 705, 954, 1092
BstMWI GCNNNNNNNGC 2 cut(s) 597, 1055
BstNI CCWGG 2 cut(s) 729, 1013
BstNSI RCATGY 3 cut(s) 743, 757, 1053
BstPAI GACNNNNGTC 1 cut(s) 38
BstSCI CCNGG 4 cut(s) 157, 727, 973, 1011
BstSFI CTRYAG 1 cut(s) 903
BstUI CGCG 1 cut(s) 164
BstV1I GCAGC 2 cut(s) 361, 866
BstV2I GAAGAC 1 cut(s) 517
BstX2I RGATCY 1 cut(s) 1092
BstYI RGATCY 1 cut(s) 1092
BsuRI GGCC 5 cut(s) 146, 152, 254, 470, 600
BtgI CCRYGG 2 cut(s) 99, 496
BtrI CACGTC 1 cut(s) 952
BtsCI GGATG 3 cut(s) 243, 439, 605
BtsI GCAGTG 1 cut(s) 823
BtsIMutI CAGTG 1 cut(s) 823
Cac8I GCNNGC 2 cut(s) 82, 256
CciI TCATGA 2 cut(s) 387, 789
CfoI GCGC 3 cut(s) 86, 164, 859
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 4 cut(s) 155, 326, 403, 598
Csp6I GTAC 3 cut(s) 285, 664, 767
CspCI CAANNNNNGTGG 2 cut(s) 958, 993
CviQI GTAC 3 cut(s) 285, 664, 767
DdeI CTNAG 4 cut(s) 21, 192, 660, 1017
DpnI GATC 6 cut(s) 140, 653, 674, 707, 956, 1094
DpnII GATC 6 cut(s) 138, 651, 672, 705, 954, 1092
EaeI YGGCCR 2 cut(s) 144, 252
Ecl136II GAGCTC 1 cut(s) 124
Eco130I CCWWGG 4 cut(s) 99, 147, 496, 930
Eco147I AGGCCT 1 cut(s) 470
Eco24I GRGCYC 1 cut(s) 126
Eco47I GGWCC 3 cut(s) 155, 326, 403
Eco47III AGCGCT 1 cut(s) 858
Eco53kI GAGCTC 1 cut(s) 124
EcoICRI GAGCTC 1 cut(s) 124
EcoNI CCTNNNNNAGG 1 cut(s) 1016
EcoO109I RGGNCCY 3 cut(s) 326, 403, 598
EcoRII CCWGG 2 cut(s) 727, 1011
EcoT14I CCWWGG 4 cut(s) 99, 147, 496, 930
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 4 cut(s) 99, 147, 496, 930
FalI AAGNNNNNCTT 2 cut(s) 867, 899
FaqI GGGAC 2 cut(s) 339, 764
FbaI TGATCA 1 cut(s) 954
Fnu4HI GCNGC 4 cut(s) 114, 375, 628, 855
FokI GGATG 3 cut(s) 250, 446, 592
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 4 cut(s) 114, 375, 628, 855
FspBI CTAG 3 cut(s) 185, 510, 918
GlaI GCGC 3 cut(s) 85, 163, 858
GluI GCNGC 4 cut(s) 114, 375, 628, 855
GsuI CTGGAG 1 cut(s) 832
HaeII RGCGCY 2 cut(s) 87, 860
HaeIII GGCC 5 cut(s) 146, 152, 254, 470, 600
HapII CCGG 9 cut(s) 12, 153, 159, 221, 235, 293, 633, 911, 975
HhaI GCGC 3 cut(s) 86, 164, 859
Hin6I GCGC 3 cut(s) 84, 162, 857
HinP1I GCGC 3 cut(s) 84, 162, 857
HincII GTYRAC 1 cut(s) 646
HindII GTYRAC 1 cut(s) 646
HinfI GANTC 5 cut(s) 19, 28, 454, 513, 880
HpaI GTTAAC 1 cut(s) 646
HpaII CCGG 9 cut(s) 12, 153, 159, 221, 235, 293, 633, 911, 975
HphI GGTGA 1 cut(s) 899
Hpy166II GTNNAC 3 cut(s) 287, 646, 767
Hpy188I TCNGA 6 cut(s) 24, 33, 46, 201, 623, 1097
Hpy8I GTNNAC 3 cut(s) 287, 646, 767
Hpy99I CGWCG 3 cut(s) 50, 413, 953
HpyAV CCTTC 2 cut(s) 257, 678
HpyCH4III ACNGT 2 cut(s) 299, 320
HpyCH4IV ACGT 1 cut(s) 951
HpyCH4V TGCA 6 cut(s) 73, 437, 736, 782, 854, 894
HpyF10VI GCNNNNNNNGC 2 cut(s) 597, 1055
HpyF3I CTNAG 4 cut(s) 21, 192, 660, 1017
HpySE526I ACGT 1 cut(s) 951
HspAI GCGC 3 cut(s) 84, 162, 857
Kpn2I TCCGGA 1 cut(s) 292
Ksp22I TGATCA 1 cut(s) 954
KspAI GTTAAC 1 cut(s) 646
Kzo9I GATC 6 cut(s) 138, 651, 672, 705, 954, 1092
LmnI GCTCC 4 cut(s) 223, 635, 721, 994
Lsp1109I GCAGC 2 cut(s) 361, 866
LweI GCATC 5 cut(s) 228, 424, 690, 703, 769
MaeI CTAG 3 cut(s) 185, 510, 918
MaeII ACGT 1 cut(s) 951
MaeIII GTNAC 2 cut(s) 803, 815
MalI GATC 6 cut(s) 140, 653, 674, 707, 956, 1094
MboI GATC 6 cut(s) 138, 651, 672, 705, 954, 1092
MboII GAAGA 5 cut(s) 368, 517, 577, 722, 853
MflI RGATCY 1 cut(s) 1092
MhlI GDGCHC 2 cut(s) 126, 825
MlsI TGGCCA 2 cut(s) 146, 254
MluCI AATT 3 cut(s) 338, 783, 833
MluNI TGGCCA 2 cut(s) 146, 254
MlyI GAGTC 2 cut(s) 13, 22
MmeI TCCRAC 5 cut(s) 56, 69, 224, 601, 724
Mox20I TGGCCA 2 cut(s) 146, 254
MroI TCCGGA 1 cut(s) 292
MscI TGGCCA 2 cut(s) 146, 254
MseI TTAA 3 cut(s) 462, 645, 1086
MslI CAYNNNNRTG 1 cut(s) 560
Msp20I TGGCCA 2 cut(s) 146, 254
MspCI CTTAAG 1 cut(s) 1085
MspI CCGG 9 cut(s) 12, 153, 159, 221, 235, 293, 633, 911, 975
MspR9I CCNGG 4 cut(s) 159, 729, 975, 1013
Mva1269I GAATGC 2 cut(s) 894, 1038
MvaI CCWGG 2 cut(s) 729, 1013
MvnI CGCG 1 cut(s) 164
MwoI GCNNNNNNNGC 2 cut(s) 597, 1055
NciI CCSGG 2 cut(s) 159, 975
NcoI CCATGG 2 cut(s) 99, 496
NdeII GATC 6 cut(s) 138, 651, 672, 705, 954, 1092
NlaIV GGNNCC 3 cut(s) 157, 327, 631
NmuCI GTSAC 2 cut(s) 803, 815
NspI RCATGY 3 cut(s) 743, 757, 1053
PagI TCATGA 2 cut(s) 387, 789
PceI AGGCCT 1 cut(s) 470
PciI ACATGT 2 cut(s) 739, 753
PctI GAATGC 2 cut(s) 894, 1038
PfeI GAWTC 3 cut(s) 454, 513, 880
PflFI GACNNNGTC 1 cut(s) 401
PflMI CCANNNNNTGG 1 cut(s) 869
PkrI GCNGC 4 cut(s) 115, 376, 629, 856
PleI GAGTC 2 cut(s) 13, 22
PpsI GAGTC 2 cut(s) 13, 22
PpuMI RGGWCCY 2 cut(s) 326, 403
PscI ACATGT 2 cut(s) 739, 753
PshAI GACNNNNGTC 1 cut(s) 38
Psp124BI GAGCTC 1 cut(s) 126
Psp5II RGGWCCY 2 cut(s) 326, 403
Psp6I CCWGG 2 cut(s) 727, 1011
PspGI CCWGG 2 cut(s) 727, 1011
PspN4I GGNNCC 3 cut(s) 157, 327, 631
PspPI GGNCC 4 cut(s) 155, 326, 403, 598
PspPPI RGGWCCY 2 cut(s) 326, 403
PsuI RGATCY 1 cut(s) 1092
PsyI GACNNNGTC 1 cut(s) 401
RsaI GTAC 3 cut(s) 286, 665, 768
RsaNI GTAC 3 cut(s) 285, 664, 767
RseI CAYNNNNRTG 1 cut(s) 560
SacI GAGCTC 1 cut(s) 126
SaqAI TTAA 3 cut(s) 462, 645, 1086
SatI GCNGC 4 cut(s) 114, 375, 628, 855
Sau3AI GATC 6 cut(s) 138, 651, 672, 705, 954, 1092
Sau96I GGNCC 4 cut(s) 155, 326, 403, 598
SchI GAGTC 2 cut(s) 13, 22
ScrFI CCNGG 4 cut(s) 159, 729, 975, 1013
SduI GDGCHC 2 cut(s) 126, 825
SfaNI GCATC 5 cut(s) 228, 424, 690, 703, 769
SfcI CTRYAG 1 cut(s) 903
SinI GGWCC 3 cut(s) 155, 326, 403
SmiMI CAYNNNNRTG 1 cut(s) 560
SmlI CTYRAG 2 cut(s) 575, 1085
SmoI CTYRAG 2 cut(s) 575, 1085
Sse9I AATT 3 cut(s) 338, 783, 833
SseBI AGGCCT 1 cut(s) 470
SsiI CCGC 2 cut(s) 114, 627
SspMI CTAG 3 cut(s) 185, 510, 918
SstI GAGCTC 1 cut(s) 126
StuI AGGCCT 1 cut(s) 470
StyD4I CCNGG 4 cut(s) 157, 727, 973, 1011
StyI CCWWGG 4 cut(s) 99, 147, 496, 930
TaaI ACNGT 2 cut(s) 299, 320
TaiI ACGT 1 cut(s) 954
TaqI TCGA 7 cut(s) 126, 411, 671, 708, 948, 1042, 1077
TasI AATT 3 cut(s) 338, 783, 833
TauI GCSGC 2 cut(s) 116, 630
TfiI GAWTC 3 cut(s) 454, 513, 880
Tru1I TTAA 3 cut(s) 462, 645, 1086
Tru9I TTAA 3 cut(s) 462, 645, 1086
TscAI CASTG 1 cut(s) 830
TseFI GTSAC 2 cut(s) 803, 815
TseI GCWGC 2 cut(s) 374, 854
Tsp45I GTSAC 2 cut(s) 803, 815
TspDTI ATGAA 4 cut(s) 404, 578, 778, 806
TspGWI ACGGA 2 cut(s) 79, 1007
TspRI CASTG 1 cut(s) 830
Tth111I GACNNNGTC 1 cut(s) 401
Van91I CCANNNNNTGG 1 cut(s) 869
Vha464I CTTAAG 1 cut(s) 1085
VpaK11BI GGWCC 3 cut(s) 155, 326, 403
XagI CCTNNNNNAGG 1 cut(s) 1016
XapI RAATTY 2 cut(s) 338, 833
XceI RCATGY 3 cut(s) 743, 757, 1053
XspI CTAG 3 cut(s) 185, 510, 918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.