RchiOBHm_Chr1g0362381

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005543
Physical Location & Seq
Forward (+)
54051453 .. 54053509
2057 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58721

Sequence Viewer

Length: 1353 bp
ATGGAGATCACTATTATCTCCAGATATACTGCGAAACCATCTTCTTCTTCACTTCAGCATCTAAAACCTCACAAACTTTCCCTCTTCGACCAGCTCACTCCAGCGACTTACGCCCCATTTGTCTTGTTCTACCCCAACTCATCATTAACTACCAATCTTTCCCAGACCTTAGTCCACTTGAAGACTTCTCTATGCGAGACCCTCACTCTCTACTACCCATTTTCCGGCCGCACCAAGAACAACCTCTACATTTATGATTTCGACGCGGGTGTCCCATACCTCGAAGCCCGAGTCAATTGTCGCCTGCTTGAGTTTCTTAAGTTTCAAGAAACCCAGCTGCTAAACGACCTCCTTCCAGTTCTTCCATTCCGGAAGGAAACGGATCACTCAGATCTGCTTCCCTTGCTTGCTTGTCAAATCAATGTTTTCGCTTGCGGTGGATTGGCAATCTCTGTCTCCGTGTCTCACAAGATTTTCGATGGGGATACGGGAGACGCTTTTCTCAAAACTTGGGCTTCTATTTTTCGCGGGGCTCGCGACGACATCGTACACCCTAATCTCTCCCAAGCCTCCCTGTTCTTCCCACCAAGAGATCCATTGCCTCAGAACTATCTGGATTTAATGGACAGCTTGTGGTTTAAGGGAAGCAATTATGTTTCAAAGAGATTTGTGTTTGATGCCAAATCCATAGCCACACTGCGAGCAAGATCGACGAGCGAAAACGTTCCCAAGCCCACACGTGTGGAGATACTAACTAGTTTTATCTGGAAACATGCAATGGCTGCTTCTAGGGCACTGTCATCTGGTACTTCACCAAAGACATCCATAGTCGTTCATGCAGTGAACATGAGACAGCGAATGAATTCACAAATGCGACCGGATACTGTCATGATCGGAAATCTTTTTTGGTGGGCCGTAGCTGCCGCCGACGCTTCTGAGGCAGACGCTCATGACTTGCGTGACTTAGTGAACATAATTAAGGAATCAATCAAGGGATTCGATAGTGAGTATTTGGAGAGTTTGCAAGGTGAAGATGGTGGATTCGGAACCATTTCTGAGTTTTGTAATCAGCTTGAGGCTATGCTTTCCTCAGAAACACCGGATATCTATGCATTCACAAGCTGGGCTAGCTTTTTTAAACAGGTTGATTTTGGTTGGGGGAGGCCATTTTGGATCGGAGCCATGGGGAAAGTTGGTCCTGCATTTAGAAATCTCACTGTGTTCGTCGACAGACAATGGGATAGAGGCATTGAAGCATGGGTGAACTTGGAGGAAAACCAAATGGCTCTGTTAGAGAAAGACCCCCATTTTTTAGCATTTGCTTCTCCAAATCCTGGAATATCAAGCATGTAA

Protein Analysis

450

Amino Acids

50.61

Weight (kDa)

5.95

Isoelectric Point (pI)

36.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 436 3.3e-75 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 269
AccB7I CCANNNNNTGG 1 cut(s) 1334
AccI GTMKAC 1 cut(s) 1227
AccII CGCG 3 cut(s) 266, 528, 537
AccIII TCCGGA 1 cut(s) 369
AciI CCGC 5 cut(s) 229, 266, 435, 528, 924
AclI AACGTT 1 cut(s) 723
AclWI GGATC 3 cut(s) 390, 587, 1181
AcoI YGGCCR 1 cut(s) 226
AcsI RAATTY 1 cut(s) 862
AcuI CTGAAG 1 cut(s) 38
AcvI CACGTG 1 cut(s) 740
AfaI GTAC 2 cut(s) 549, 808
AfiI CCNNNNNNNGG 2 cut(s) 224, 1334
AflII CTTAAG 1 cut(s) 317
AflIII ACRYGT 2 cut(s) 737, 739
AgsI TTSAA 4 cut(s) 181, 326, 660, 1253
AhlI ACTAGT 1 cut(s) 755
AjnI CCWGG 1 cut(s) 1333
AjuI GAANNNNNNNTTGG 2 cut(s) 889, 921
AleI CACNNNNGTG 1 cut(s) 740
AluBI AGCT 7 cut(s) 94, 337, 630, 920, 1072, 1122, 1131
AluI AGCT 7 cut(s) 94, 337, 630, 920, 1072, 1122, 1131
Alw26I GTCTC 5 cut(s) 191, 460, 468, 486, 844
AlwI GGATC 3 cut(s) 390, 587, 1181
Ama87I CYCGRG 1 cut(s) 288
Aor13HI TCCGGA 1 cut(s) 369
AoxI GGCC 3 cut(s) 226, 912, 1163
ApeKI GCWGC 3 cut(s) 337, 782, 920
ApoI RAATTY 1 cut(s) 862
ArsI GACNNNNNNTTYG 2 cut(s) 276, 308
Asp700I GAANNNNTTC 3 cut(s) 723, 862, 1051
AspS9I GGNCC 2 cut(s) 912, 1196
AsuHPI GGTGA 3 cut(s) 804, 1040, 1273
AsuNHI GCTAGC 1 cut(s) 1127
AvaI CYCGRG 1 cut(s) 288
AvaII GGWCC 1 cut(s) 1196
BaeGI GKGCMC 1 cut(s) 796
BanII GRGCYC 1 cut(s) 535
BbrPI CACGTG 1 cut(s) 740
BbsI GAAGAC 1 cut(s) 188
BbvI GCAGC 3 cut(s) 324, 769, 907
BccI CCATC 3 cut(s) 46, 473, 1028
BceAI ACGGC 1 cut(s) 899
BciT130I CCWGG 1 cut(s) 1335
BciVI GTATCC 2 cut(s) 478, 874
BcoDI GTCTC 5 cut(s) 191, 460, 468, 486, 844
BcuI ACTAGT 1 cut(s) 755
BfaI CTAG 3 cut(s) 756, 789, 1128
BfrI CTTAAG 1 cut(s) 317
BfuI GTATCC 2 cut(s) 478, 874
BglII AGATCT 1 cut(s) 391
BisI GCNGC 5 cut(s) 229, 338, 783, 921, 924
BlsI GCNGC 5 cut(s) 230, 339, 784, 922, 925
Bme1390I CCNGG 1 cut(s) 1335
Bme18I GGWCC 1 cut(s) 1196
BmeT110I CYCGRG 1 cut(s) 288
BmgT120I GGNCC 2 cut(s) 912, 1196
BmiI GGNNCC 2 cut(s) 1048, 1180
BmrFI CCNGG 1 cut(s) 1335
BmsI GCATC 2 cut(s) 67, 667
BmtI GCTAGC 1 cut(s) 1131
BoxI GACNNNNGTC 1 cut(s) 170
BpiI GAAGAC 1 cut(s) 188
BpmI CTGGAG 2 cut(s) 4, 84
BpuEI CTTGAG 2 cut(s) 329, 1094
BsaAI YACGTR 1 cut(s) 740
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 1182
BsaWI WCCGGW 3 cut(s) 369, 877, 1099
Bsc4I CCNNNNNNNGG 2 cut(s) 224, 1334
Bse1I ACTGG 1 cut(s) 356
Bse3DI GCAATG 2 cut(s) 596, 783
BseAI TCCGGA 1 cut(s) 369
BseBI CCWGG 1 cut(s) 1335
BseDI CCNNGG 1 cut(s) 1182
BseGI GGATG 1 cut(s) 821
BseLI CCNNNNNNNGG 2 cut(s) 224, 1334
BseMI GCAATG 2 cut(s) 596, 783
BseMII CTCAG 5 cut(s) 402, 617, 927, 1047, 1104
BseNI ACTGG 1 cut(s) 356
BseSI GKGCMC 1 cut(s) 796
BseX3I CGGCCG 1 cut(s) 226
BseXI GCAGC 3 cut(s) 324, 769, 907
BseYI CCCAGC 2 cut(s) 333, 1122
Bsh1236I CGCG 3 cut(s) 266, 528, 537
Bsh1285I CGRYCG 2 cut(s) 229, 878
BshFI GGCC 3 cut(s) 228, 914, 1165
BsiEI CGRYCG 2 cut(s) 229, 878
BsiHKCI CYCGRG 1 cut(s) 288
BsiSI CCGG 4 cut(s) 225, 370, 878, 1100
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 2 cut(s) 224, 1334
BsmAI GTCTC 5 cut(s) 191, 460, 468, 486, 844
BsmBI CGTCTC 1 cut(s) 486
BsmFI GGGAC 1 cut(s) 257
BsmI GAATGC 1 cut(s) 1112
BsnI GGCC 3 cut(s) 228, 914, 1165
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 1 cut(s) 288
Bsp1286I GDGCHC 2 cut(s) 535, 796
Bsp13I TCCGGA 1 cut(s) 369
Bsp143I GATC 7 cut(s) 6, 382, 391, 592, 707, 891, 1173
Bsp19I CCATGG 1 cut(s) 1182
Bsp68I TCGCGA 1 cut(s) 537
BspACI CCGC 5 cut(s) 229, 266, 435, 528, 924
BspANI GGCC 3 cut(s) 228, 914, 1165
BspCNI CTCAG 5 cut(s) 401, 616, 928, 1048, 1103
BspEI TCCGGA 1 cut(s) 369
BspFNI CGCG 3 cut(s) 266, 528, 537
BspHI TCATGA 2 cut(s) 888, 949
BspLI GGNNCC 2 cut(s) 1048, 1180
BspOI GCTAGC 1 cut(s) 1131
BspPI GGATC 3 cut(s) 390, 587, 1181
BspTI CTTAAG 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 191
BsrDI GCAATG 2 cut(s) 596, 783
BsrI ACTGG 1 cut(s) 356
BssECI CCNNGG 1 cut(s) 1182
BssMI GATC 7 cut(s) 6, 382, 391, 592, 707, 891, 1173
BssT1I CCWWGG 1 cut(s) 1182
Bst2UI CCWGG 1 cut(s) 1335
Bst4CI ACNGT 3 cut(s) 798, 886, 1219
Bst6I CTCTTC 1 cut(s) 89
BstAFI CTTAAG 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 782
BstBAI YACGTR 1 cut(s) 740
BstC8I GCNNGC 6 cut(s) 305, 408, 433, 535, 702, 1129
BstDEI CTNAG 7 cut(s) 169, 388, 603, 936, 964, 1056, 1090
BstDSI CCRYGG 1 cut(s) 1182
BstF5I GGATG 1 cut(s) 821
BstFNI CGCG 3 cut(s) 266, 528, 537
BstKTI GATC 7 cut(s) 9, 385, 394, 595, 710, 894, 1176
BstMAI GTCTC 5 cut(s) 191, 460, 468, 486, 844
BstMBI GATC 7 cut(s) 6, 382, 391, 592, 707, 891, 1173
BstMCI CGRYCG 2 cut(s) 229, 878
BstMWI GCNNNNNNNGC 9 cut(s) 110, 403, 534, 782, 791, 920, 929, 938, 1128
BstNI CCWGG 1 cut(s) 1335
BstNSI RCATGY 2 cut(s) 776, 1351
BstPAI GACNNNNGTC 1 cut(s) 170
BstSCI CCNGG 1 cut(s) 1333
BstSLI GKGCMC 1 cut(s) 796
BstUI CGCG 3 cut(s) 266, 528, 537
BstV1I GCAGC 3 cut(s) 324, 769, 907
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 2 cut(s) 391, 592
BstXI CCANNNNNNTGG 1 cut(s) 742
BstYI RGATCY 2 cut(s) 391, 592
BstZI CGGCCG 1 cut(s) 226
BsuI GTATCC 2 cut(s) 478, 874
BsuRI GGCC 3 cut(s) 228, 914, 1165
BtgI CCRYGG 1 cut(s) 1182
BtsCI GGATG 1 cut(s) 821
BtsI GCAGTG 2 cut(s) 695, 846
BtsIMutI CAGTG 4 cut(s) 695, 794, 846, 1215
BtuMI TCGCGA 1 cut(s) 537
Cac8I GCNNGC 6 cut(s) 305, 408, 433, 535, 702, 1129
CciI TCATGA 2 cut(s) 888, 949
Cfr13I GGNCC 2 cut(s) 912, 1196
CseI GACGC 4 cut(s) 272, 503, 938, 953
Csp6I GTAC 2 cut(s) 548, 807
CviAII CATG 8 cut(s) 773, 836, 847, 889, 950, 1183, 1257, 1348
CviQI GTAC 2 cut(s) 548, 807
DdeI CTNAG 7 cut(s) 169, 388, 603, 936, 964, 1056, 1090
DpnI GATC 7 cut(s) 8, 384, 393, 594, 709, 893, 1175
DpnII GATC 7 cut(s) 6, 382, 391, 592, 707, 891, 1173
DraI TTTAAA 1 cut(s) 1138
DrdI GACNNNNNNGTC 1 cut(s) 269
DseDI GACNNNNNNGTC 1 cut(s) 269
EaeI YGGCCR 1 cut(s) 226
EagI CGGCCG 1 cut(s) 226
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
EclXI CGGCCG 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 1182
Eco24I GRGCYC 1 cut(s) 535
Eco31I GGTCTC 1 cut(s) 191
Eco32I GATATC 1 cut(s) 1105
Eco47I GGWCC 1 cut(s) 1196
Eco52I CGGCCG 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 38
Eco72I CACGTG 1 cut(s) 740
Eco88I CYCGRG 1 cut(s) 288
EcoRI GAATTC 1 cut(s) 862
EcoRII CCWGG 1 cut(s) 1333
EcoRV GATATC 1 cut(s) 1105
EcoT14I CCWWGG 1 cut(s) 1182
EcoT22I ATGCAT 1 cut(s) 1114
EcoT38I GRGCYC 1 cut(s) 535
ErhI CCWWGG 1 cut(s) 1182
Esp3I CGTCTC 1 cut(s) 486
FaeI CATG 8 cut(s) 776, 839, 850, 892, 953, 1186, 1260, 1351
FaqI GGGAC 1 cut(s) 257
FatI CATG 8 cut(s) 772, 835, 846, 888, 949, 1182, 1256, 1347
FauI CCCGC 2 cut(s) 259, 521
FblI GTMKAC 1 cut(s) 1227
Fnu4HI GCNGC 5 cut(s) 229, 338, 783, 921, 924
FokI GGATG 1 cut(s) 808
FriOI GRGCYC 1 cut(s) 535
Fsp4HI GCNGC 5 cut(s) 229, 338, 783, 921, 924
FspBI CTAG 3 cut(s) 756, 789, 1128
GluI GCNGC 5 cut(s) 229, 338, 783, 921, 924
GsaI CCCAGC 2 cut(s) 337, 1126
GsuI CTGGAG 2 cut(s) 4, 84
HaeIII GGCC 3 cut(s) 228, 914, 1165
HapII CCGG 4 cut(s) 225, 370, 878, 1100
HgaI GACGC 4 cut(s) 272, 503, 938, 953
Hin1II CATG 8 cut(s) 776, 839, 850, 892, 953, 1186, 1260, 1351
HincII GTYRAC 1 cut(s) 1228
HindII GTYRAC 1 cut(s) 1228
HinfI GANTC 4 cut(s) 291, 983, 996, 1041
HpaII CCGG 4 cut(s) 225, 370, 878, 1100
HphI GGTGA 3 cut(s) 804, 1040, 1273
Hpy166II GTNNAC 6 cut(s) 175, 550, 844, 970, 1228, 1264
Hpy188I TCNGA 8 cut(s) 391, 606, 896, 937, 1046, 1057, 1093, 1178
Hpy188III TCNNGA 8 cut(s) 21, 326, 370, 536, 614, 766, 889, 950
Hpy8I GTNNAC 6 cut(s) 175, 550, 844, 970, 1228, 1264
Hpy99I CGWCG 5 cut(s) 266, 542, 715, 932, 1229
HpyAV CCTTC 2 cut(s) 362, 367
HpyCH4III ACNGT 3 cut(s) 798, 886, 1219
HpyCH4IV ACGT 2 cut(s) 723, 739
HpyCH4V TGCA 5 cut(s) 776, 839, 1024, 1112, 1202
HpyF10VI GCNNNNNNNGC 9 cut(s) 110, 403, 534, 782, 791, 920, 929, 938, 1128
HpyF3I CTNAG 7 cut(s) 169, 388, 603, 936, 964, 1056, 1090
HpySE526I ACGT 2 cut(s) 723, 739
Hsp92II CATG 8 cut(s) 776, 839, 850, 892, 953, 1186, 1260, 1351
Kpn2I TCCGGA 1 cut(s) 369
Kzo9I GATC 7 cut(s) 6, 382, 391, 592, 707, 891, 1173
LmnI GCTCC 1 cut(s) 1178
Lsp1109I GCAGC 3 cut(s) 324, 769, 907
LweI GCATC 2 cut(s) 67, 667
MaeI CTAG 3 cut(s) 756, 789, 1128
MaeII ACGT 2 cut(s) 723, 739
MaeIII GTNAC 1 cut(s) 959
MalI GATC 7 cut(s) 8, 384, 393, 594, 709, 893, 1175
MboI GATC 7 cut(s) 6, 382, 391, 592, 707, 891, 1173
MboII GAAGA 8 cut(s) 33, 36, 39, 76, 193, 353, 571, 1043
MfeI CAATTG 1 cut(s) 295
MflI RGATCY 2 cut(s) 391, 592
MhlI GDGCHC 2 cut(s) 535, 796
MluCI AATT 4 cut(s) 295, 649, 862, 975
MlyI GAGTC 1 cut(s) 300
Mph1103I ATGCAT 1 cut(s) 1114
MroI TCCGGA 1 cut(s) 369
MroXI GAANNNNTTC 3 cut(s) 723, 862, 1051
MseI TTAA 6 cut(s) 146, 318, 620, 639, 978, 1137
MslI CAYNNNNRTG 1 cut(s) 740
MspA1I CMGCKG 1 cut(s) 337
MspCI CTTAAG 1 cut(s) 317
MspI CCGG 4 cut(s) 225, 370, 878, 1100
MspR9I CCNGG 1 cut(s) 1335
MunI CAATTG 1 cut(s) 295
Mva1269I GAATGC 1 cut(s) 1112
MvaI CCWGG 1 cut(s) 1335
MvnI CGCG 3 cut(s) 266, 528, 537
MwoI GCNNNNNNNGC 9 cut(s) 110, 403, 534, 782, 791, 920, 929, 938, 1128
NcoI CCATGG 1 cut(s) 1182
NdeII GATC 7 cut(s) 6, 382, 391, 592, 707, 891, 1173
NheI GCTAGC 1 cut(s) 1127
NlaIII CATG 8 cut(s) 776, 839, 850, 892, 953, 1186, 1260, 1351
NlaIV GGNNCC 2 cut(s) 1048, 1180
NmuCI GTSAC 1 cut(s) 959
NruI TCGCGA 1 cut(s) 537
NsiI ATGCAT 1 cut(s) 1114
NspI RCATGY 2 cut(s) 776, 1351
OliI CACNNNNGTG 1 cut(s) 740
PagI TCATGA 2 cut(s) 888, 949
PctI GAATGC 1 cut(s) 1112
PdmI GAANNNNTTC 3 cut(s) 723, 862, 1051
PfeI GAWTC 3 cut(s) 983, 996, 1041
PflMI CCANNNNNTGG 1 cut(s) 1334
PfoI TCCNGGA 1 cut(s) 1333
PkrI GCNGC 5 cut(s) 230, 339, 784, 922, 925
PleI GAGTC 1 cut(s) 299
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 1 cut(s) 299
Ppu21I YACGTR 1 cut(s) 740
PshAI GACNNNNGTC 1 cut(s) 170
Psp1406I AACGTT 1 cut(s) 723
Psp6I CCWGG 1 cut(s) 1333
PspCI CACGTG 1 cut(s) 740
PspFI CCCAGC 2 cut(s) 333, 1122
PspGI CCWGG 1 cut(s) 1333
PspN4I GGNNCC 2 cut(s) 1048, 1180
PspPI GGNCC 2 cut(s) 912, 1196
PsrI GAACNNNNNNTAC 2 cut(s) 230, 262
PsuI RGATCY 2 cut(s) 391, 592
PvuII CAGCTG 1 cut(s) 337
RruI TCGCGA 1 cut(s) 537
RsaI GTAC 2 cut(s) 549, 808
RsaNI GTAC 2 cut(s) 548, 807
RseI CAYNNNNRTG 1 cut(s) 740
SalI GTCGAC 1 cut(s) 1226
SaqAI TTAA 6 cut(s) 146, 318, 620, 639, 978, 1137
SatI GCNGC 5 cut(s) 229, 338, 783, 921, 924
Sau3AI GATC 7 cut(s) 6, 382, 391, 592, 707, 891, 1173
Sau96I GGNCC 2 cut(s) 912, 1196
SchI GAGTC 1 cut(s) 300
ScrFI CCNGG 1 cut(s) 1335
SduI GDGCHC 2 cut(s) 535, 796
SfaNI GCATC 2 cut(s) 67, 667
SinI GGWCC 1 cut(s) 1196
SmiMI CAYNNNNRTG 1 cut(s) 740
SmlI CTYRAG 3 cut(s) 308, 317, 1073
SmoI CTYRAG 3 cut(s) 308, 317, 1073
SpeI ACTAGT 1 cut(s) 755
Sse9I AATT 4 cut(s) 295, 649, 862, 975
SsiI CCGC 5 cut(s) 229, 266, 435, 528, 924
SspMI CTAG 3 cut(s) 756, 789, 1128
StyD4I CCNGG 1 cut(s) 1333
StyI CCWWGG 1 cut(s) 1182
TaaI ACNGT 3 cut(s) 798, 886, 1219
TaiI ACGT 2 cut(s) 726, 742
TaqI TCGA 7 cut(s) 87, 261, 282, 477, 710, 999, 1227
TasI AATT 4 cut(s) 295, 649, 862, 975
TauI GCSGC 2 cut(s) 231, 926
TfiI GAWTC 3 cut(s) 983, 996, 1041
Tru1I TTAA 6 cut(s) 146, 318, 620, 639, 978, 1137
Tru9I TTAA 6 cut(s) 146, 318, 620, 639, 978, 1137
TscAI CASTG 4 cut(s) 702, 801, 846, 1222
TseFI GTSAC 1 cut(s) 959
TseI GCWGC 3 cut(s) 337, 782, 920
Tsp45I GTSAC 1 cut(s) 959
TspDTI ATGAA 2 cut(s) 824, 875
TspGWI ACGGA 2 cut(s) 395, 448
TspRI CASTG 4 cut(s) 702, 801, 846, 1222
Van91I CCANNNNNTGG 1 cut(s) 1334
Vha464I CTTAAG 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 1196
XapI RAATTY 1 cut(s) 862
XceI RCATGY 2 cut(s) 776, 1351
XmiI GTMKAC 1 cut(s) 1227
XmnI GAANNNNTTC 3 cut(s) 723, 862, 1051
XspI CTAG 3 cut(s) 756, 789, 1128
Zsp2I ATGCAT 1 cut(s) 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.