Rw4G013470

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005543
Physical Location & Seq
Reverse (-)
31424739 .. 31426663
1925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G013470.1

Sequence Viewer

Length: 1380 bp
ATGGTGAACACCATGGAGATCAGTATCATTTCTAGAGAGACCATCAAACCATCGCTCGATTCCTCTTCACTTAACCATCTAAGTCCTTACAAACTTTCCCTCATCGACCAGCTGACTCCCGCAACTTATATGCCACTGATCTTGTTCTACCCCATTAGTGATCCCAAGTCATCGCTCAATCTCCTCGAAACCCTAACCCGCTTAAAAAGCTCCCTCTCCGTGACTCTCACTCTCTACTATCCATTTTCGGGACGGGCTAAAAACAACCTCGACATTCATGACTTTGACACCGGTATTCCCTTCCTTGAAGCCCGAGTCAATTGTCGCATGCTCGAGTTTCTTAAGCTCCAAGAAACTGAGTTGCTGAACCTTTTCGTTCCATTCCATCCCATTTGCAAGGAAACTGGTGATGAGTCTCTGCTCCCCATGATAGCTTTTCAAGTGAATGTCTTTGCTTGTGGTGGAATAGCAATTGAGGGATCAGCTTCTCACAAGCTATGTGATAGGTTGGCCGCGAACTCTATCCTTGAGTCTTGGTCTACTATTTTTCGTGGCGGGGAGTGCGACAAAATAATACACCCTAATTTCTCCCAAGCCTCATTGCTCTTTCCTCCAAGAGCTCTGTTGCCTAAAAAGTATCTAGATTTAGCAGATAGCTTTTGGTTCAAGGAAAACAATTATGTTACAAGGAGATTTGTGTTTAGTGTCAAAGCCATAGCCACACTACAAGAAAAATCGAAGAGCAAATGCGTGCCCAAGCCATCACGTAATGACGCACTCACATGTTTTATTTGGAAACATGCAATGGCTTCTTCTTGGGCACTGTCACCAGGTTCCTCACCAAGAACTTCCATAGTAGCGCATGCAATGAACATGAGATCAAGGATGAAGCCACAAACGTCGTCAGCTAATATCGTTGGAAATTTATTTTGGTGGGCGACAACAGTCGCGGACACATCTAAGCTAGGGGAAGATCATGAGTTATGTGACTTGGTGCACCTGATTAATGAATCTCTCAAAGGATTTGATAGGGGTTATTTTGAGTCTTTTCAGGGAGAAGAGGGATTTGGAGCCATTTCAAAATACTTTAATCAACTAGAAGTCTTGCTTTCTTCAAAATCCCCACCAGAACTTTATTTATTCACCAATTGGACAAGCATTTTTCACAAATTGGATTTTGGATGGGGGAAGCCTATCTGGGTAGGGCTCATGGGGAAAGTCGGGCCTGAGTTTAGGAATTTCACGGTGTTCATGGAAGCACCATGTGGTAAAGGAATTGAAGCGTGGGTGACATTGGAGGCAAAGCAAATGGCTCTGCTCCAAAAAGACCTTAAGTTTCTAGCATTTGCTTCTCCAAAATCTGGGATTTCAAGCATGTGA

Protein Analysis

459

Amino Acids

51.54

Weight (kDa)

8.26

Isoelectric Point (pI)

36.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 445 5.3e-61 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1361
AccI GTMKAC 1 cut(s) 539
AccII CGCG 2 cut(s) 515, 950
AciI CCGC 5 cut(s) 120, 199, 513, 555, 950
AclWI GGATC 2 cut(s) 155, 487
AcoI YGGCCR 1 cut(s) 510
AcsI RAATTY 2 cut(s) 922, 1237
AdeI CACNNNGTG 1 cut(s) 1265
AfiI CCNNNNNNNGG 2 cut(s) 248, 1361
AflII CTTAAG 2 cut(s) 341, 1331
AflIII ACRYGT 1 cut(s) 782
AgeI ACCGGT 1 cut(s) 290
AgsI TTSAA 7 cut(s) 308, 440, 667, 1080, 1116, 1280, 1371
AjnI CCWGG 1 cut(s) 829
AjuI GAANNNNNNNTTGG 4 cut(s) 913, 945, 1050, 1082
Alw21I GWGCWC 2 cut(s) 622, 999
Alw26I GTCTC 2 cut(s) 32, 420
Alw44I GTGCAC 1 cut(s) 995
AlwI GGATC 2 cut(s) 155, 487
Ama87I CYCGRG 2 cut(s) 312, 332
AoxI GGCC 2 cut(s) 510, 1223
ApaLI GTGCAC 1 cut(s) 995
ApoI RAATTY 2 cut(s) 922, 1237
AseI ATTAAT 1 cut(s) 1005
AsiGI ACCGGT 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 371
AspLEI GCGC 1 cut(s) 862
AspS9I GGNCC 1 cut(s) 1223
AsuHPI GGTGA 6 cut(s) 16, 419, 819, 831, 1135, 1300
AvaI CYCGRG 2 cut(s) 312, 332
BaeGI GKGCMC 3 cut(s) 756, 823, 999
BanII GRGCYC 2 cut(s) 622, 1209
Bbv12I GWGCWC 2 cut(s) 622, 999
BccI CCATC 6 cut(s) 50, 58, 84, 393, 769, 1176
BciT130I CCWGG 1 cut(s) 831
BcoDI GTCTC 2 cut(s) 32, 420
BfaI CTAG 5 cut(s) 33, 641, 965, 1097, 1340
BfrI CTTAAG 2 cut(s) 341, 1331
BisI GCNGC 1 cut(s) 513
BlsI GCNGC 1 cut(s) 514
Bme1390I CCNGG 1 cut(s) 831
BmeT110I CYCGRG 2 cut(s) 312, 332
BmgT120I GGNCC 1 cut(s) 1223
BmiI GGNNCC 2 cut(s) 835, 1072
BmrFI CCNGG 1 cut(s) 831
BoxI GACNNNNGTC 1 cut(s) 944
BpuEI CTTGAG 1 cut(s) 548
BsaAI YACGTR 1 cut(s) 767
BsaBI GATNNNNATC 1 cut(s) 23
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 12
BsaWI WCCGGW 1 cut(s) 290
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 1361
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 409
Bse3DI GCAATG 3 cut(s) 599, 810, 873
Bse8I GATNNNNATC 1 cut(s) 23
BseBI CCWGG 1 cut(s) 831
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 3 cut(s) 385, 891, 1187
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 2 cut(s) 248, 1361
BseMI GCAATG 3 cut(s) 599, 810, 873
BseMII CTCAG 2 cut(s) 348, 1218
BseNI ACTGG 1 cut(s) 409
BseRI GAGGAG 1 cut(s) 173
BseSI GKGCMC 3 cut(s) 756, 823, 999
Bsh1236I CGCG 2 cut(s) 515, 950
BshFI GGCC 2 cut(s) 512, 1225
BshTI ACCGGT 1 cut(s) 290
BsiHKAI GWGCWC 2 cut(s) 622, 999
BsiHKCI CYCGRG 2 cut(s) 312, 332
BsiSI CCGG 1 cut(s) 291
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 2 cut(s) 248, 1361
BsmAI GTCTC 2 cut(s) 32, 420
BsmFI GGGAC 1 cut(s) 264
BsnI GGCC 2 cut(s) 512, 1225
Bso31I GGTCTC 1 cut(s) 32
BsoBI CYCGRG 2 cut(s) 312, 332
Bsp1286I GDGCHC 5 cut(s) 622, 756, 823, 999, 1209
Bsp143I GATC 6 cut(s) 18, 138, 160, 479, 878, 973
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 5 cut(s) 120, 199, 513, 555, 950
BspANI GGCC 2 cut(s) 512, 1225
BspCNI CTCAG 2 cut(s) 349, 1219
BspFNI CGCG 2 cut(s) 515, 950
BspHI TCATGA 2 cut(s) 277, 976
BspLI GGNNCC 2 cut(s) 835, 1072
BspPI GGATC 2 cut(s) 155, 487
BspQI GCTCTTC 1 cut(s) 734
BspTI CTTAAG 2 cut(s) 341, 1331
BspTNI GGTCTC 1 cut(s) 32
BsrDI GCAATG 3 cut(s) 599, 810, 873
BsrFI RCCGGY 1 cut(s) 290
BsrI ACTGG 1 cut(s) 409
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 6 cut(s) 18, 138, 160, 479, 878, 973
BssT1I CCWWGG 1 cut(s) 12
Bst2UI CCWGG 1 cut(s) 831
Bst4CI ACNGT 3 cut(s) 825, 946, 1246
Bst6I CTCTTC 3 cut(s) 70, 734, 1053
BstAFI CTTAAG 2 cut(s) 341, 1331
BstBAI YACGTR 1 cut(s) 767
BstC8I GCNNGC 3 cut(s) 329, 752, 864
BstDEI CTNAG 4 cut(s) 80, 357, 960, 1227
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 3 cut(s) 385, 891, 1187
BstFNI CGCG 2 cut(s) 515, 950
BstHHI GCGC 1 cut(s) 862
BstKTI GATC 6 cut(s) 21, 141, 163, 482, 881, 976
BstMAI GTCTC 2 cut(s) 32, 420
BstMBI GATC 6 cut(s) 18, 138, 160, 479, 878, 973
BstMWI GCNNNNNNNGC 2 cut(s) 207, 561
BstNI CCWGG 1 cut(s) 831
BstNSI RCATGY 5 cut(s) 331, 786, 803, 866, 1378
BstPAI GACNNNNGTC 1 cut(s) 944
BstSCI CCNGG 1 cut(s) 829
BstSLI GKGCMC 3 cut(s) 756, 823, 999
BstUI CGCG 2 cut(s) 515, 950
BsuRI GGCC 2 cut(s) 512, 1225
BtgI CCRYGG 1 cut(s) 12
BtgZI GCGATG 2 cut(s) 36, 156
BtsCI GGATG 3 cut(s) 385, 891, 1187
BtsIMutI CAGTG 2 cut(s) 134, 821
Cac8I GCNNGC 3 cut(s) 329, 752, 864
CciI TCATGA 2 cut(s) 277, 976
CfoI GCGC 1 cut(s) 862
Cfr10I RCCGGY 1 cut(s) 290
Cfr13I GGNCC 1 cut(s) 1223
CseI GACGC 1 cut(s) 782
CsiI ACCWGGT 1 cut(s) 829
CspAI ACCGGT 1 cut(s) 290
CspCI CAANNNNNGTGG 2 cut(s) 123, 158
DdeI CTNAG 4 cut(s) 80, 357, 960, 1227
DpnI GATC 6 cut(s) 20, 140, 162, 481, 880, 975
DpnII GATC 6 cut(s) 18, 138, 160, 479, 878, 973
DraIII CACNNNGTG 1 cut(s) 1265
EaeI YGGCCR 1 cut(s) 510
Eam1104I CTCTTC 3 cut(s) 70, 734, 1053
EarI CTCTTC 3 cut(s) 70, 734, 1053
Ecl136II GAGCTC 1 cut(s) 620
Eco130I CCWWGG 1 cut(s) 12
Eco24I GRGCYC 2 cut(s) 622, 1209
Eco31I GGTCTC 1 cut(s) 32
Eco53kI GAGCTC 1 cut(s) 620
Eco88I CYCGRG 2 cut(s) 312, 332
EcoICRI GAGCTC 1 cut(s) 620
EcoRII CCWGG 1 cut(s) 829
EcoT14I CCWWGG 1 cut(s) 12
EcoT38I GRGCYC 2 cut(s) 622, 1209
ErhI CCWWGG 1 cut(s) 12
FalI AAGNNNNNCTT 2 cut(s) 1092, 1124
FaqI GGGAC 1 cut(s) 264
FauI CCCGC 3 cut(s) 127, 206, 548
FblI GTMKAC 1 cut(s) 539
Fnu4HI GCNGC 1 cut(s) 513
FokI GGATG 3 cut(s) 372, 898, 1194
FriOI GRGCYC 2 cut(s) 622, 1209
Fsp4HI GCNGC 1 cut(s) 513
FspBI CTAG 5 cut(s) 33, 641, 965, 1097, 1340
GlaI GCGC 1 cut(s) 861
GluI GCNGC 1 cut(s) 513
HaeIII GGCC 2 cut(s) 512, 1225
HapII CCGG 1 cut(s) 291
HgaI GACGC 1 cut(s) 782
HhaI GCGC 1 cut(s) 862
Hin6I GCGC 1 cut(s) 860
HinP1I GCGC 1 cut(s) 860
HinfI GANTC 8 cut(s) 59, 115, 223, 315, 413, 530, 1010, 1043
HpaII CCGG 1 cut(s) 291
HphI GGTGA 6 cut(s) 16, 419, 819, 831, 1135, 1300
Hpy166II GTNNAC 3 cut(s) 7, 540, 997
Hpy188III TCNNGA 5 cut(s) 33, 249, 278, 641, 977
Hpy8I GTNNAC 3 cut(s) 7, 540, 997
Hpy99I CGWCG 1 cut(s) 904
HpyAV CCTTC 1 cut(s) 310
HpyCH4III ACNGT 3 cut(s) 825, 946, 1246
HpyCH4IV ACGT 2 cut(s) 766, 899
HpyCH4V TGCA 4 cut(s) 396, 803, 866, 997
HpyF10VI GCNNNNNNNGC 2 cut(s) 207, 561
HpyF3I CTNAG 4 cut(s) 80, 357, 960, 1227
HpySE526I ACGT 2 cut(s) 766, 899
HspAI GCGC 1 cut(s) 860
Kzo9I GATC 6 cut(s) 18, 138, 160, 479, 878, 973
LguI GCTCTTC 1 cut(s) 734
LmnI GCTCC 5 cut(s) 215, 351, 426, 1070, 1323
MabI ACCWGGT 1 cut(s) 829
MaeI CTAG 5 cut(s) 33, 641, 965, 1097, 1340
MaeII ACGT 2 cut(s) 766, 899
MaeIII GTNAC 5 cut(s) 220, 682, 825, 986, 1288
MalI GATC 6 cut(s) 20, 140, 162, 481, 880, 975
MboI GATC 6 cut(s) 18, 138, 160, 479, 878, 973
MboII GAAGA 6 cut(s) 57, 751, 804, 983, 1070, 1104
MfeI CAATTG 3 cut(s) 319, 471, 1147
MhlI GDGCHC 5 cut(s) 622, 756, 823, 999, 1209
MluCI AATT 9 cut(s) 319, 471, 583, 676, 922, 1147, 1169, 1237, 1275
MlyI GAGTC 6 cut(s) 109, 217, 324, 422, 539, 1052
MmeI TCCRAC 1 cut(s) 898
MroXI GAANNNNTTC 1 cut(s) 371
MseI TTAA 6 cut(s) 72, 203, 342, 1005, 1089, 1332
MslI CAYNNNNRTG 1 cut(s) 781
MspA1I CMGCKG 1 cut(s) 112
MspCI CTTAAG 2 cut(s) 341, 1331
MspI CCGG 1 cut(s) 291
MspR9I CCNGG 1 cut(s) 831
MunI CAATTG 3 cut(s) 319, 471, 1147
MvaI CCWGG 1 cut(s) 831
MvnI CGCG 2 cut(s) 515, 950
MwoI GCNNNNNNNGC 2 cut(s) 207, 561
NcoI CCATGG 1 cut(s) 12
NdeII GATC 6 cut(s) 18, 138, 160, 479, 878, 973
NlaIV GGNNCC 2 cut(s) 835, 1072
NmuCI GTSAC 4 cut(s) 220, 825, 986, 1288
NspI RCATGY 5 cut(s) 331, 786, 803, 866, 1378
PaeI GCATGC 2 cut(s) 331, 866
PaeR7I CTCGAG 1 cut(s) 332
PagI TCATGA 2 cut(s) 277, 976
PciI ACATGT 1 cut(s) 782
PciSI GCTCTTC 1 cut(s) 734
PdmI GAANNNNTTC 1 cut(s) 371
PfeI GAWTC 2 cut(s) 59, 1010
PflMI CCANNNNNTGG 1 cut(s) 1361
PinAI ACCGGT 1 cut(s) 290
PkrI GCNGC 1 cut(s) 514
PleI GAGTC 6 cut(s) 109, 217, 323, 421, 538, 1051
PpsI GAGTC 6 cut(s) 109, 217, 323, 421, 538, 1051
Ppu21I YACGTR 1 cut(s) 767
PscI ACATGT 1 cut(s) 782
PshAI GACNNNNGTC 1 cut(s) 944
PshBI ATTAAT 1 cut(s) 1005
Psp124BI GAGCTC 1 cut(s) 622
Psp6I CCWGG 1 cut(s) 829
PspGI CCWGG 1 cut(s) 829
PspN4I GGNNCC 2 cut(s) 835, 1072
PspPI GGNCC 1 cut(s) 1223
PspXI VCTCGAGB 1 cut(s) 332
PvuII CAGCTG 1 cut(s) 112
RseI CAYNNNNRTG 1 cut(s) 781
SacI GAGCTC 1 cut(s) 622
SapI GCTCTTC 1 cut(s) 734
SaqAI TTAA 6 cut(s) 72, 203, 342, 1005, 1089, 1332
SatI GCNGC 1 cut(s) 513
Sau3AI GATC 6 cut(s) 18, 138, 160, 479, 878, 973
Sau96I GGNCC 1 cut(s) 1223
SchI GAGTC 6 cut(s) 109, 217, 324, 422, 539, 1052
ScrFI CCNGG 1 cut(s) 831
SduI GDGCHC 5 cut(s) 622, 756, 823, 999, 1209
SexAI ACCWGGT 1 cut(s) 829
Sfr274I CTCGAG 1 cut(s) 332
SlaI CTCGAG 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 781
SmlI CTYRAG 4 cut(s) 332, 341, 527, 1331
SmoI CTYRAG 4 cut(s) 332, 341, 527, 1331
SphI GCATGC 2 cut(s) 331, 866
Sse9I AATT 9 cut(s) 319, 471, 583, 676, 922, 1147, 1169, 1237, 1275
SsiI CCGC 5 cut(s) 120, 199, 513, 555, 950
SspMI CTAG 5 cut(s) 33, 641, 965, 1097, 1340
SstI GAGCTC 1 cut(s) 622
StyD4I CCNGG 1 cut(s) 829
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 3 cut(s) 825, 946, 1246
TaiI ACGT 2 cut(s) 769, 902
TaqI TCGA 6 cut(s) 57, 105, 186, 270, 333, 737
TasI AATT 9 cut(s) 319, 471, 583, 676, 922, 1147, 1169, 1237, 1275
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 2 cut(s) 59, 1010
Tru1I TTAA 6 cut(s) 72, 203, 342, 1005, 1089, 1332
Tru9I TTAA 6 cut(s) 72, 203, 342, 1005, 1089, 1332
TscAI CASTG 2 cut(s) 141, 828
TseFI GTSAC 4 cut(s) 220, 825, 986, 1288
Tsp45I GTSAC 4 cut(s) 220, 825, 986, 1288
TspDTI ATGAA 5 cut(s) 266, 884, 902, 1023, 1240
TspGWI ACGGA 1 cut(s) 208
TspRI CASTG 2 cut(s) 141, 828
Van91I CCANNNNNTGG 1 cut(s) 1361
Vha464I CTTAAG 2 cut(s) 341, 1331
VneI GTGCAC 1 cut(s) 995
VspI ATTAAT 1 cut(s) 1005
XapI RAATTY 2 cut(s) 922, 1237
XbaI TCTAGA 2 cut(s) 32, 640
XceI RCATGY 5 cut(s) 331, 786, 803, 866, 1378
XhoI CTCGAG 1 cut(s) 332
XmiI GTMKAC 1 cut(s) 539
XmnI GAANNNNTTC 1 cut(s) 371
XspI CTAG 5 cut(s) 33, 641, 965, 1097, 1340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.