Rroxscaffold_5G00355160

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005543
Physical Location & Seq
Reverse (-)
33759039 .. 33760573
1535 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00355160.1

Sequence Viewer

Length: 1368 bp
ATGGTGAACACCATGGAGGTCAGTATCATTTCCAGAGACACCATCAAACCATCGCTCGATTCCTCTTCACTTAACCATCTAAGTCCTTTCAAACTTTCCCTCATCGACCAGCTGACTCCCGCAACTTATATGCCACTGATCTTGTTCTACCCCATTAGTGATCCCAAGTCATCGTTCAATCTCCTCGAAACCCTAACCCGATTAAAAAACTCCCTCTCCGAGACTCTCACTCTCTACTATCCACTTTCGGGACGGGCTAAAAACAACCTCGTCATTCATGACTTTCACACCGGTATTCCCTTCCTTGAAGCCCGAGTCAATTGTCGCATGCTCGAGTTTCTTAAGCTCCAAGAAACTGAGTTGCTGAACCTTTTCGTTCCATTCCATCCCATTTGCAAGGAAACTGGTGATGAGTCTCTGCTCCCCATGATAGCTTTTCAAGTGAATGTCTTTGCTTGTGGTGGAATAGCAATTGGGGGATCCGCTTCTCACAAGCTATGTGATGGGTTGGCCGCGAACTCTCTCCTTGAGTCTTGGTCTGCTATTTTTCGTGGGAAGTGCGACAAAATAATACACCCTAATTTCTCCCAAGCCTCATCGCTCTTTCCTCCAAGAGCTCTGTTGCCTAAAAGGTATCTAGATTTAGTAGATAGCTTTTGGTTCAAGGAAAACAATTATGTTACAAGGAGATTTGTGTTTAGTGTCAAAGCCATAGCCACACTACAAGAAAAATCGAAGAGCAAATGCGTGCCCAAGCCATCACGTAATGACACACTCACATGTTTTATTTGGAAACATGCAACGGCTTCTTCTCGGGCACTGTCACCAGGTTCCTCACCAAGAACTTCCATAGTAGCGCATTCAATGAATATAAGATCACTGATGAAGCCACAAACATTGTCAGCTAATATCATTGGAAATTTCTTTTGGTGGGCAACAACAGTCGAGGACTCATCTAAGCTAGGAGAAGATCATGAGTTATGTGACTTGGTGCACCTGATTAATGAATCTCTCAAAGGATTTGATAGGGGTTATTTTGAGTCTCATCAGGGAGAAGAGGGATTTGGAGGCATTTCAAACTACTTTAATCAACTAGAAGTCTTGCTTTCTTCAAAATCCCCACCAGAACTTTATTTATTCACCAATTGGACAAGCATTTTTCACAAATTGGATTTTGGATGGGGGAAGCCTTTTTGGGTAGGGCTCATGGGGAAAGTCAGGCGTGAGTTTAGGAATTTCATGGAAGCACAATGTTGTAAAGGAATTGAAGCGAGGGTGACATTGGAGGCAAAGCAAATGGCTCTGCTCCAAAAAGACCTTAAGTTTCTAGCATTTGCTTCTCCAAATTTTGGGATTTCAAGCATGTGA

Protein Analysis

455

Amino Acids

51.27

Weight (kDa)

8.69

Isoelectric Point (pI)

38.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 5 - 441 2.6e-62 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1349
AccII CGCG 1 cut(s) 515
AciI CCGC 3 cut(s) 120, 483, 513
AclWI GGATC 3 cut(s) 155, 474, 487
AcoI YGGCCR 1 cut(s) 510
AcsI RAATTY 3 cut(s) 919, 1234, 1345
AfiI CCNNNNNNNGG 2 cut(s) 248, 1349
AflII CTTAAG 2 cut(s) 341, 1319
AflIII ACRYGT 1 cut(s) 779
AgeI ACCGGT 1 cut(s) 290
AjnI CCWGG 1 cut(s) 826
AjuI GAANNNNNNNTTGG 6 cut(s) 158, 190, 910, 942, 1047, 1079
AluBI AGCT 8 cut(s) 112, 346, 434, 496, 617, 654, 905, 961
AluI AGCT 8 cut(s) 112, 346, 434, 496, 617, 654, 905, 961
Alw21I GWGCWC 2 cut(s) 619, 996
Alw26I GTCTC 4 cut(s) 30, 215, 420, 1047
Alw44I GTGCAC 1 cut(s) 992
AlwI GGATC 3 cut(s) 155, 474, 487
Ama87I CYCGRG 3 cut(s) 312, 332, 813
AoxI GGCC 1 cut(s) 510
ApaLI GTGCAC 1 cut(s) 992
ApoI RAATTY 3 cut(s) 919, 1234, 1345
AseI ATTAAT 1 cut(s) 1002
AsiGI ACCGGT 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 371
AspLEI GCGC 1 cut(s) 859
AsuHPI GGTGA 6 cut(s) 16, 419, 816, 828, 1132, 1288
AvaI CYCGRG 3 cut(s) 312, 332, 813
BaeGI GKGCMC 3 cut(s) 753, 820, 996
BamHI GGATCC 1 cut(s) 479
BanII GRGCYC 2 cut(s) 619, 1206
Bbv12I GWGCWC 2 cut(s) 619, 996
BccI CCATC 7 cut(s) 50, 58, 84, 393, 497, 766, 1173
BceAI ACGGC 1 cut(s) 819
BciT130I CCWGG 1 cut(s) 828
BcoDI GTCTC 4 cut(s) 30, 215, 420, 1047
BfaI CTAG 4 cut(s) 638, 962, 1094, 1328
BfrI CTTAAG 2 cut(s) 341, 1319
BisI GCNGC 1 cut(s) 513
BlsI GCNGC 1 cut(s) 514
Bme1390I CCNGG 1 cut(s) 828
BmeT110I CYCGRG 3 cut(s) 312, 332, 813
BmiI GGNNCC 2 cut(s) 481, 832
BmrFI CCNGG 1 cut(s) 828
BpuEI CTTGAG 1 cut(s) 548
BsaAI YACGTR 1 cut(s) 764
BsaJI CCNNGG 1 cut(s) 12
BsaWI WCCGGW 1 cut(s) 290
BsaXI ACNNNNNCTCC 2 cut(s) 200, 230
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 1349
Bse118I RCCGGY 1 cut(s) 290
Bse1I ACTGG 1 cut(s) 409
BseBI CCWGG 1 cut(s) 828
BseDI CCNNGG 1 cut(s) 12
BseGI GGATG 2 cut(s) 385, 1184
BseLI CCNNNNNNNGG 2 cut(s) 248, 1349
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 1 cut(s) 409
BseRI GAGGAG 1 cut(s) 173
BseSI GKGCMC 3 cut(s) 753, 820, 996
Bsh1236I CGCG 1 cut(s) 515
BshFI GGCC 1 cut(s) 512
BshTI ACCGGT 1 cut(s) 290
BsiHKAI GWGCWC 2 cut(s) 619, 996
BsiHKCI CYCGRG 3 cut(s) 312, 332, 813
BsiSI CCGG 1 cut(s) 291
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 2 cut(s) 248, 1349
BsmAI GTCTC 4 cut(s) 30, 215, 420, 1047
BsmFI GGGAC 1 cut(s) 264
BsmI GAATGC 1 cut(s) 859
BsnI GGCC 1 cut(s) 512
BsoBI CYCGRG 3 cut(s) 312, 332, 813
Bsp1286I GDGCHC 5 cut(s) 619, 753, 820, 996, 1206
Bsp143I GATC 5 cut(s) 138, 160, 479, 875, 970
Bsp19I CCATGG 1 cut(s) 12
BspACI CCGC 3 cut(s) 120, 483, 513
BspANI GGCC 1 cut(s) 512
BspCNI CTCAG 1 cut(s) 349
BspFNI CGCG 1 cut(s) 515
BspHI TCATGA 2 cut(s) 277, 973
BspLI GGNNCC 2 cut(s) 481, 832
BspPI GGATC 3 cut(s) 155, 474, 487
BspQI GCTCTTC 1 cut(s) 731
BspTI CTTAAG 2 cut(s) 341, 1319
BsrFI RCCGGY 1 cut(s) 290
BsrI ACTGG 1 cut(s) 409
BssAI RCCGGY 1 cut(s) 290
BssECI CCNNGG 1 cut(s) 12
BssMI GATC 5 cut(s) 138, 160, 479, 875, 970
BssT1I CCWWGG 1 cut(s) 12
Bst2UI CCWGG 1 cut(s) 828
Bst4CI ACNGT 2 cut(s) 822, 943
Bst6I CTCTTC 3 cut(s) 70, 731, 1050
BstAFI CTTAAG 2 cut(s) 341, 1319
BstBAI YACGTR 1 cut(s) 764
BstC8I GCNNGC 2 cut(s) 329, 749
BstDEI CTNAG 3 cut(s) 80, 357, 957
BstDSI CCRYGG 1 cut(s) 12
BstF5I GGATG 2 cut(s) 385, 1184
BstFNI CGCG 1 cut(s) 515
BstHHI GCGC 1 cut(s) 859
BstKTI GATC 5 cut(s) 141, 163, 482, 878, 973
BstMAI GTCTC 4 cut(s) 30, 215, 420, 1047
BstMBI GATC 5 cut(s) 138, 160, 479, 875, 970
BstNI CCWGG 1 cut(s) 828
BstNSI RCATGY 4 cut(s) 331, 783, 800, 1366
BstSCI CCNGG 1 cut(s) 826
BstSLI GKGCMC 3 cut(s) 753, 820, 996
BstUI CGCG 1 cut(s) 515
BstX2I RGATCY 1 cut(s) 479
BstYI RGATCY 1 cut(s) 479
BsuRI GGCC 1 cut(s) 512
BtgI CCRYGG 1 cut(s) 12
BtgZI GCGATG 2 cut(s) 36, 582
BtsCI GGATG 2 cut(s) 385, 1184
BtsIMutI CAGTG 3 cut(s) 134, 818, 878
Cac8I GCNNGC 2 cut(s) 329, 749
CciI TCATGA 2 cut(s) 277, 973
CfoI GCGC 1 cut(s) 859
Cfr10I RCCGGY 1 cut(s) 290
CsiI ACCWGGT 1 cut(s) 826
CspAI ACCGGT 1 cut(s) 290
CspCI CAANNNNNGTGG 4 cut(s) 123, 158, 879, 914
DdeI CTNAG 3 cut(s) 80, 357, 957
DpnI GATC 5 cut(s) 140, 162, 481, 877, 972
DpnII GATC 5 cut(s) 138, 160, 479, 875, 970
EaeI YGGCCR 1 cut(s) 510
Eam1104I CTCTTC 3 cut(s) 70, 731, 1050
EarI CTCTTC 3 cut(s) 70, 731, 1050
Ecl136II GAGCTC 1 cut(s) 617
Eco130I CCWWGG 1 cut(s) 12
Eco24I GRGCYC 2 cut(s) 619, 1206
Eco53kI GAGCTC 1 cut(s) 617
Eco88I CYCGRG 3 cut(s) 312, 332, 813
EcoICRI GAGCTC 1 cut(s) 617
EcoRII CCWGG 1 cut(s) 826
EcoT14I CCWWGG 1 cut(s) 12
EcoT38I GRGCYC 2 cut(s) 619, 1206
ErhI CCWWGG 1 cut(s) 12
FalI AAGNNNNNCTT 2 cut(s) 1089, 1121
FaqI GGGAC 1 cut(s) 264
FauI CCCGC 1 cut(s) 127
Fnu4HI GCNGC 1 cut(s) 513
FokI GGATG 2 cut(s) 372, 1191
FriOI GRGCYC 2 cut(s) 619, 1206
Fsp4HI GCNGC 1 cut(s) 513
FspBI CTAG 4 cut(s) 638, 962, 1094, 1328
GlaI GCGC 1 cut(s) 858
GluI GCNGC 1 cut(s) 513
HaeIII GGCC 1 cut(s) 512
HapII CCGG 1 cut(s) 291
HhaI GCGC 1 cut(s) 859
Hin6I GCGC 1 cut(s) 857
HinP1I GCGC 1 cut(s) 857
HinfI GANTC 9 cut(s) 59, 115, 223, 315, 413, 530, 950, 1007, 1040
HpaII CCGG 1 cut(s) 291
HphI GGTGA 6 cut(s) 16, 419, 816, 828, 1132, 1288
Hpy166II GTNNAC 2 cut(s) 7, 994
Hpy188I TCNGA 1 cut(s) 220
Hpy188III TCNNGA 5 cut(s) 33, 249, 278, 638, 974
Hpy8I GTNNAC 2 cut(s) 7, 994
HpyAV CCTTC 1 cut(s) 310
HpyCH4III ACNGT 2 cut(s) 822, 943
HpyCH4IV ACGT 1 cut(s) 763
HpyCH4V TGCA 3 cut(s) 396, 800, 994
HpyF3I CTNAG 3 cut(s) 80, 357, 957
HpySE526I ACGT 1 cut(s) 763
HspAI GCGC 1 cut(s) 857
Kzo9I GATC 5 cut(s) 138, 160, 479, 875, 970
LguI GCTCTTC 1 cut(s) 731
LmnI GCTCC 3 cut(s) 351, 426, 1311
MabI ACCWGGT 1 cut(s) 826
MaeI CTAG 4 cut(s) 638, 962, 1094, 1328
MaeII ACGT 1 cut(s) 763
MaeIII GTNAC 4 cut(s) 679, 822, 983, 1276
MalI GATC 5 cut(s) 140, 162, 481, 877, 972
MboI GATC 5 cut(s) 138, 160, 479, 875, 970
MboII GAAGA 6 cut(s) 57, 748, 801, 980, 1067, 1101
MfeI CAATTG 3 cut(s) 319, 471, 1144
MflI RGATCY 1 cut(s) 479
MhlI GDGCHC 5 cut(s) 619, 753, 820, 996, 1206
MlyI GAGTC 7 cut(s) 109, 217, 324, 422, 539, 944, 1049
MroXI GAANNNNTTC 1 cut(s) 371
MseI TTAA 6 cut(s) 72, 203, 342, 1002, 1086, 1320
MslI CAYNNNNRTG 1 cut(s) 778
MspA1I CMGCKG 1 cut(s) 112
MspCI CTTAAG 2 cut(s) 341, 1319
MspI CCGG 1 cut(s) 291
MspR9I CCNGG 1 cut(s) 828
MunI CAATTG 3 cut(s) 319, 471, 1144
Mva1269I GAATGC 1 cut(s) 859
MvaI CCWGG 1 cut(s) 828
MvnI CGCG 1 cut(s) 515
NcoI CCATGG 1 cut(s) 12
NdeII GATC 5 cut(s) 138, 160, 479, 875, 970
NlaIV GGNNCC 2 cut(s) 481, 832
NmuCI GTSAC 3 cut(s) 822, 983, 1276
NspI RCATGY 4 cut(s) 331, 783, 800, 1366
PaeI GCATGC 1 cut(s) 331
PaeR7I CTCGAG 1 cut(s) 332
PagI TCATGA 2 cut(s) 277, 973
PciI ACATGT 1 cut(s) 779
PciSI GCTCTTC 1 cut(s) 731
PctI GAATGC 1 cut(s) 859
PdmI GAANNNNTTC 1 cut(s) 371
PfeI GAWTC 2 cut(s) 59, 1007
PflMI CCANNNNNTGG 1 cut(s) 1349
PinAI ACCGGT 1 cut(s) 290
PkrI GCNGC 1 cut(s) 514
PleI GAGTC 7 cut(s) 109, 217, 323, 421, 538, 944, 1048
PpsI GAGTC 7 cut(s) 109, 217, 323, 421, 538, 944, 1048
Ppu21I YACGTR 1 cut(s) 764
PscI ACATGT 1 cut(s) 779
PshBI ATTAAT 1 cut(s) 1002
Psp124BI GAGCTC 1 cut(s) 619
Psp6I CCWGG 1 cut(s) 826
PspGI CCWGG 1 cut(s) 826
PspN4I GGNNCC 2 cut(s) 481, 832
PspXI VCTCGAGB 1 cut(s) 332
PsuI RGATCY 1 cut(s) 479
PvuII CAGCTG 1 cut(s) 112
RseI CAYNNNNRTG 1 cut(s) 778
SacI GAGCTC 1 cut(s) 619
SapI GCTCTTC 1 cut(s) 731
SaqAI TTAA 6 cut(s) 72, 203, 342, 1002, 1086, 1320
SatI GCNGC 1 cut(s) 513
Sau3AI GATC 5 cut(s) 138, 160, 479, 875, 970
SchI GAGTC 7 cut(s) 109, 217, 324, 422, 539, 944, 1049
ScrFI CCNGG 1 cut(s) 828
SduI GDGCHC 5 cut(s) 619, 753, 820, 996, 1206
SexAI ACCWGGT 1 cut(s) 826
Sfr274I CTCGAG 1 cut(s) 332
SlaI CTCGAG 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 778
SmlI CTYRAG 4 cut(s) 332, 341, 527, 1319
SmoI CTYRAG 4 cut(s) 332, 341, 527, 1319
SphI GCATGC 1 cut(s) 331
SsiI CCGC 3 cut(s) 120, 483, 513
SspMI CTAG 4 cut(s) 638, 962, 1094, 1328
SstI GAGCTC 1 cut(s) 619
StyD4I CCNGG 1 cut(s) 826
StyI CCWWGG 1 cut(s) 12
TaaI ACNGT 2 cut(s) 822, 943
TaiI ACGT 1 cut(s) 766
TaqI TCGA 6 cut(s) 57, 105, 186, 333, 734, 945
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 2 cut(s) 59, 1007
Tru1I TTAA 6 cut(s) 72, 203, 342, 1002, 1086, 1320
Tru9I TTAA 6 cut(s) 72, 203, 342, 1002, 1086, 1320
TscAI CASTG 3 cut(s) 141, 825, 885
TseFI GTSAC 3 cut(s) 822, 983, 1276
Tsp45I GTSAC 3 cut(s) 822, 983, 1276
TspDTI ATGAA 5 cut(s) 266, 881, 899, 1020, 1228
TspRI CASTG 3 cut(s) 141, 825, 885
Van91I CCANNNNNTGG 1 cut(s) 1349
Vha464I CTTAAG 2 cut(s) 341, 1319
VneI GTGCAC 1 cut(s) 992
VspI ATTAAT 1 cut(s) 1002
XapI RAATTY 3 cut(s) 919, 1234, 1345
XbaI TCTAGA 1 cut(s) 637
XceI RCATGY 4 cut(s) 331, 783, 800, 1366
XhoI CTCGAG 1 cut(s) 332
XmnI GAANNNNTTC 1 cut(s) 371
XspI CTAG 4 cut(s) 638, 962, 1094, 1328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.