RLG00000018584

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005543
Physical Location & Seq
Forward (+)
32201158 .. 32202601
1444 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018584

Sequence Viewer

Length: 1353 bp
ATGGAGATCACTATTATCTCCAGAGATACAGCGAAACCATCTTCCTCTTCACTTCAGCATCTAAAACCTCACAAACTTTCCCTCTTCGACCAGCTCACTCCCGCGACTTACGCCCCAATTGTCTTGTTCTACCCCAACTCATCATTAACTACCAATCTTTCCCAGACCTTAATCCACTTGAAGACTTCTCTGTGCGAGACCCTCACTCTCTACTACCCATTTTCCGGCCGCACCAAGAACAACCTATACATTTTTGATTTCGACGCCGGTGTTCCATACCTCGAAGCCCGAGTCAATTGTCGCCTGCCTGAGTTTCATAAGCTTCAACAAACCCAGCTGCTAAACGACCTCCTTCCATTTCTTCCATTCCGGAAGGAAACGGATCACTCAGACCTGCTTCCCTTGCTTGCTTGTCAAATCAATGTTTTCGCTTGCGGTGGATTAGCAATCTCTGTCTCCTTTTCTCACAAGATTTTCGATGGGGATACGGCAAACGCTTTTCTCAAAACTTGGGCGGCTGTTTTTCGCGGGGCTCGCGCCGACATTATACACCCTAATCTCTCCCAAGCCTCCCTGCTCTTCCAACCAAGAGATTCGTTGCCTCAGAACTATCTAGATTTAATGGATAGCTTGTGGTTTAAGGGAAGCAATTATGTTTCAAAGAGATTTGTGTTTGATGCCAAATCCATAGCCACACTGCGAGCAAGATCGACGAGCGAAAACGTTCCCAAGCCCACACGTGTGGAGATACTGACTAGTTTTCTCTGGAAACATGCAATGGCTGCTTCTAGGGCATTGTCATCCGGTACTTCACCAAAGACATCCATAGTCGTTCATGCAGTGAACATGAGGCAGCGAATGAATTCACAAATGGGATCGGGTGATGTCATGATCGGAAATCTTTTTTGGTGGGCCTTAGCTGCCGCCGACGCATCTGAGGCAGACGCTCATGACTTGTGTGACTTAGTGAACCTAATTAAGGAATCAATCAAAGGATTCGATAGTGAGTATTTGGAGAGTTTGCGAGGTGAAGATGGTGGATTTGGAACTATTTCCGAGTTTTGTAATCAGCTTGAGGCTATGCTTTCCTCAGAAACACCAGATATCTATGCATTCACAAGCTGGGTTAGCTTTTTTAATCAGGTTGATTTTGGTTGGGGGAGGCCATTTTGGATCGGAGTCACGGGGAAAGTTGGTCCTGCATTTAGAAATCTTACTCTGTTTGTCGAGAGACAATGGGATAGGGGCATTGAAGCATGGGTGAACTTGGAGGAGAACCAAATGGCTCTGTTAGAGAAAGACCCCCAGTTTTTAGCATTTGCTTCTCCAAATCCTGGAATTTCAAGCATGTAA

Protein Analysis

451

Amino Acids

50.4

Weight (kDa)

5.95

Isoelectric Point (pI)

38.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 437 7.9e-74 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 402
AccB7I CCANNNNNTGG 1 cut(s) 1334
AccII CGCG 3 cut(s) 104, 528, 537
AccIII TCCGGA 1 cut(s) 369
AciI CCGC 6 cut(s) 102, 229, 435, 515, 528, 924
AclI AACGTT 1 cut(s) 723
AclWI GGATC 3 cut(s) 390, 883, 1181
AcoI YGGCCR 1 cut(s) 226
AcsI RAATTY 2 cut(s) 862, 1338
AcuI CTGAAG 1 cut(s) 38
AcvI CACGTG 1 cut(s) 740
AcyI GRCGYC 1 cut(s) 264
AfaI GTAC 1 cut(s) 808
AfiI CCNNNNNNNGG 3 cut(s) 224, 979, 1334
AflIII ACRYGT 2 cut(s) 737, 739
AgsI TTSAA 5 cut(s) 181, 326, 660, 1253, 1344
AhlI ACTAGT 1 cut(s) 755
AjnI CCWGG 1 cut(s) 1333
AjuI GAANNNNNNNTTGG 2 cut(s) 889, 921
AleI CACNNNNGTG 1 cut(s) 740
AluBI AGCT 8 cut(s) 94, 322, 337, 630, 920, 1072, 1122, 1131
AluI AGCT 8 cut(s) 94, 322, 337, 630, 920, 1072, 1122, 1131
Alw26I GTCTC 3 cut(s) 191, 460, 1225
AlwI GGATC 3 cut(s) 390, 883, 1181
Ama87I CYCGRG 1 cut(s) 288
Aor13HI TCCGGA 1 cut(s) 369
AoxI GGCC 3 cut(s) 226, 912, 1163
ApeKI GCWGC 4 cut(s) 337, 782, 853, 920
ApoI RAATTY 2 cut(s) 862, 1338
ArsI GACNNNNNNTTYG 2 cut(s) 276, 308
Asp700I GAANNNNTTC 3 cut(s) 723, 862, 1051
AspLEI GCGC 1 cut(s) 539
AspS9I GGNCC 2 cut(s) 912, 1196
AsuHPI GGTGA 4 cut(s) 804, 893, 1040, 1273
AvaI CYCGRG 1 cut(s) 288
AvaII GGWCC 1 cut(s) 1196
BanII GRGCYC 1 cut(s) 535
BbrPI CACGTG 1 cut(s) 740
BbsI GAAGAC 1 cut(s) 188
BbvI GCAGC 4 cut(s) 324, 769, 865, 907
BccI CCATC 3 cut(s) 46, 473, 1028
BceAI ACGGC 1 cut(s) 504
BciT130I CCWGG 1 cut(s) 1335
BciVI GTATCC 1 cut(s) 478
BcoDI GTCTC 3 cut(s) 191, 460, 1225
BcuI ACTAGT 1 cut(s) 755
BfaI CTAG 3 cut(s) 614, 756, 789
BfuAI ACCTGC 1 cut(s) 402
BfuI GTATCC 1 cut(s) 478
BisI GCNGC 7 cut(s) 229, 338, 516, 783, 854, 921, 924
BlsI GCNGC 7 cut(s) 230, 339, 517, 784, 855, 922, 925
Bme1390I CCNGG 1 cut(s) 1335
Bme18I GGWCC 1 cut(s) 1196
BmeT110I CYCGRG 1 cut(s) 288
BmgT120I GGNCC 2 cut(s) 912, 1196
BmrFI CCNGG 1 cut(s) 1335
BmrI ACTGGG 1 cut(s) 1300
BmsI GCATC 3 cut(s) 67, 667, 941
BmuI ACTGGG 1 cut(s) 1300
BpiI GAAGAC 1 cut(s) 188
BpmI CTGGAG 1 cut(s) 4
Bpu10I CCTNAGC 1 cut(s) 916
BpuEI CTTGAG 1 cut(s) 1094
BsaAI YACGTR 1 cut(s) 740
BsaHI GRCGYC 1 cut(s) 264
BsaI GGTCTC 1 cut(s) 191
BsaWI WCCGGW 2 cut(s) 369, 803
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 979, 1334
Bse118I RCCGGY 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 1306
Bse3DI GCAATG 1 cut(s) 783
BseAI TCCGGA 1 cut(s) 369
BseBI CCWGG 1 cut(s) 1335
BseGI GGATG 2 cut(s) 800, 821
BseLI CCNNNNNNNGG 3 cut(s) 224, 979, 1334
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 5 cut(s) 300, 402, 617, 927, 1104
BseNI ACTGG 1 cut(s) 1306
BseRI GAGGAG 1 cut(s) 1286
BseX3I CGGCCG 1 cut(s) 226
BseXI GCAGC 4 cut(s) 324, 769, 865, 907
BseYI CCCAGC 2 cut(s) 333, 1122
Bsh1236I CGCG 3 cut(s) 104, 528, 537
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 3 cut(s) 228, 914, 1165
BsiEI CGRYCG 1 cut(s) 229
BsiHKCI CYCGRG 1 cut(s) 288
BsiSI CCGG 4 cut(s) 225, 267, 370, 804
BslI CCNNNNNNNGG 3 cut(s) 224, 979, 1334
BsmAI GTCTC 3 cut(s) 191, 460, 1225
BsmI GAATGC 1 cut(s) 1112
BsnI GGCC 3 cut(s) 228, 914, 1165
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 535
Bsp13I TCCGGA 1 cut(s) 369
Bsp143I GATC 6 cut(s) 6, 382, 707, 875, 891, 1173
BspACI CCGC 6 cut(s) 102, 229, 435, 515, 528, 924
BspANI GGCC 3 cut(s) 228, 914, 1165
BspCNI CTCAG 5 cut(s) 301, 401, 616, 928, 1103
BspEI TCCGGA 1 cut(s) 369
BspFNI CGCG 3 cut(s) 104, 528, 537
BspHI TCATGA 2 cut(s) 888, 949
BspMI ACCTGC 1 cut(s) 402
BspPI GGATC 3 cut(s) 390, 883, 1181
BspQI GCTCTTC 1 cut(s) 584
BspTNI GGTCTC 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 783
BsrFI RCCGGY 1 cut(s) 266
BsrI ACTGG 1 cut(s) 1306
BssAI RCCGGY 1 cut(s) 266
BssMI GATC 6 cut(s) 6, 382, 707, 875, 891, 1173
BssNI GRCGYC 1 cut(s) 264
Bst2UI CCWGG 1 cut(s) 1335
Bst6I CTCTTC 3 cut(s) 52, 89, 584
BstACI GRCGYC 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 782
BstBAI YACGTR 1 cut(s) 740
BstC8I GCNNGC 5 cut(s) 305, 408, 433, 535, 702
BstDEI CTNAG 7 cut(s) 309, 388, 603, 916, 936, 964, 1090
BstENI CCTNNNNNAGG 1 cut(s) 977
BstF5I GGATG 2 cut(s) 800, 821
BstFNI CGCG 3 cut(s) 104, 528, 537
BstHHI GCGC 1 cut(s) 539
BstKTI GATC 6 cut(s) 9, 385, 710, 878, 894, 1176
BstMAI GTCTC 3 cut(s) 191, 460, 1225
BstMBI GATC 6 cut(s) 6, 382, 707, 875, 891, 1173
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 9 cut(s) 110, 403, 534, 782, 791, 920, 929, 938, 1128
BstNI CCWGG 1 cut(s) 1335
BstNSI RCATGY 2 cut(s) 776, 1351
BstSCI CCNGG 1 cut(s) 1333
BstUI CGCG 3 cut(s) 104, 528, 537
BstV1I GCAGC 4 cut(s) 324, 769, 865, 907
BstV2I GAAGAC 1 cut(s) 188
BstXI CCANNNNNNTGG 1 cut(s) 742
BstZI CGGCCG 1 cut(s) 226
BsuI GTATCC 1 cut(s) 478
BsuRI GGCC 3 cut(s) 228, 914, 1165
BtsCI GGATG 2 cut(s) 800, 821
BtsI GCAGTG 2 cut(s) 695, 846
BtsIMutI CAGTG 2 cut(s) 695, 846
BveI ACCTGC 1 cut(s) 402
Cac8I GCNNGC 5 cut(s) 305, 408, 433, 535, 702
CciI TCATGA 2 cut(s) 888, 949
CfoI GCGC 1 cut(s) 539
Cfr10I RCCGGY 1 cut(s) 266
Cfr13I GGNCC 2 cut(s) 912, 1196
CseI GACGC 3 cut(s) 272, 938, 953
Csp6I GTAC 1 cut(s) 807
CviAII CATG 7 cut(s) 773, 836, 847, 889, 950, 1257, 1348
CviQI GTAC 1 cut(s) 807
DdeI CTNAG 7 cut(s) 309, 388, 603, 916, 936, 964, 1090
DpnI GATC 6 cut(s) 8, 384, 709, 877, 893, 1175
DpnII GATC 6 cut(s) 6, 382, 707, 875, 891, 1173
EaeI YGGCCR 1 cut(s) 226
EagI CGGCCG 1 cut(s) 226
Eam1104I CTCTTC 3 cut(s) 52, 89, 584
EarI CTCTTC 3 cut(s) 52, 89, 584
EclXI CGGCCG 1 cut(s) 226
Eco24I GRGCYC 1 cut(s) 535
Eco31I GGTCTC 1 cut(s) 191
Eco32I GATATC 1 cut(s) 1105
Eco47I GGWCC 1 cut(s) 1196
Eco52I CGGCCG 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 38
Eco72I CACGTG 1 cut(s) 740
Eco88I CYCGRG 1 cut(s) 288
EcoNI CCTNNNNNAGG 1 cut(s) 977
EcoRI GAATTC 1 cut(s) 862
EcoRII CCWGG 1 cut(s) 1333
EcoRV GATATC 1 cut(s) 1105
EcoT22I ATGCAT 1 cut(s) 1114
EcoT38I GRGCYC 1 cut(s) 535
FaeI CATG 7 cut(s) 776, 839, 850, 892, 953, 1260, 1351
FatI CATG 7 cut(s) 772, 835, 846, 888, 949, 1256, 1347
FauI CCCGC 2 cut(s) 109, 521
Fnu4HI GCNGC 7 cut(s) 229, 338, 516, 783, 854, 921, 924
FokI GGATG 2 cut(s) 787, 808
FriOI GRGCYC 1 cut(s) 535
Fsp4HI GCNGC 7 cut(s) 229, 338, 516, 783, 854, 921, 924
FspBI CTAG 3 cut(s) 614, 756, 789
GlaI GCGC 1 cut(s) 538
GluI GCNGC 7 cut(s) 229, 338, 516, 783, 854, 921, 924
GsaI CCCAGC 2 cut(s) 337, 1126
GsuI CTGGAG 1 cut(s) 4
HaeIII GGCC 3 cut(s) 228, 914, 1165
HapII CCGG 4 cut(s) 225, 267, 370, 804
HgaI GACGC 3 cut(s) 272, 938, 953
HhaI GCGC 1 cut(s) 539
Hin1I GRCGYC 1 cut(s) 264
Hin1II CATG 7 cut(s) 776, 839, 850, 892, 953, 1260, 1351
Hin6I GCGC 1 cut(s) 537
HinP1I GCGC 1 cut(s) 537
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 5 cut(s) 291, 593, 983, 996, 1179
HpaII CCGG 4 cut(s) 225, 267, 370, 804
HphI GGTGA 4 cut(s) 804, 893, 1040, 1273
Hpy166II GTNNAC 3 cut(s) 844, 970, 1264
Hpy188I TCNGA 7 cut(s) 391, 606, 896, 937, 1057, 1093, 1178
Hpy188III TCNNGA 7 cut(s) 21, 370, 614, 766, 889, 950, 1228
Hpy8I GTNNAC 3 cut(s) 844, 970, 1264
Hpy99I CGWCG 3 cut(s) 266, 715, 932
HpyAV CCTTC 2 cut(s) 362, 367
HpyCH4IV ACGT 2 cut(s) 723, 739
HpyCH4V TGCA 4 cut(s) 776, 839, 1112, 1202
HpyF10VI GCNNNNNNNGC 9 cut(s) 110, 403, 534, 782, 791, 920, 929, 938, 1128
HpyF3I CTNAG 7 cut(s) 309, 388, 603, 916, 936, 964, 1090
HpySE526I ACGT 2 cut(s) 723, 739
Hsp92I GRCGYC 1 cut(s) 264
Hsp92II CATG 7 cut(s) 776, 839, 850, 892, 953, 1260, 1351
HspAI GCGC 1 cut(s) 537
Kpn2I TCCGGA 1 cut(s) 369
Kzo9I GATC 6 cut(s) 6, 382, 707, 875, 891, 1173
LguI GCTCTTC 1 cut(s) 584
Lsp1109I GCAGC 4 cut(s) 324, 769, 865, 907
LweI GCATC 3 cut(s) 67, 667, 941
MaeI CTAG 3 cut(s) 614, 756, 789
MaeII ACGT 2 cut(s) 723, 739
MaeIII GTNAC 2 cut(s) 959, 1180
MalI GATC 6 cut(s) 8, 384, 709, 877, 893, 1175
MboI GATC 6 cut(s) 6, 382, 707, 875, 891, 1173
MboII GAAGA 7 cut(s) 33, 39, 76, 193, 353, 571, 1043
MfeI CAATTG 2 cut(s) 117, 295
MhlI GDGCHC 1 cut(s) 535
MluCI AATT 6 cut(s) 117, 295, 649, 862, 975, 1338
MlyI GAGTC 2 cut(s) 300, 1188
MmeI TCCRAC 1 cut(s) 607
Mph1103I ATGCAT 1 cut(s) 1114
MroI TCCGGA 1 cut(s) 369
MroXI GAANNNNTTC 3 cut(s) 723, 862, 1051
MseI TTAA 6 cut(s) 146, 170, 620, 639, 978, 1137
MslI CAYNNNNRTG 1 cut(s) 740
MspA1I CMGCKG 1 cut(s) 337
MspI CCGG 4 cut(s) 225, 267, 370, 804
MspR9I CCNGG 1 cut(s) 1335
MunI CAATTG 2 cut(s) 117, 295
Mva1269I GAATGC 1 cut(s) 1112
MvaI CCWGG 1 cut(s) 1335
MvnI CGCG 3 cut(s) 104, 528, 537
MwoI GCNNNNNNNGC 9 cut(s) 110, 403, 534, 782, 791, 920, 929, 938, 1128
NdeII GATC 6 cut(s) 6, 382, 707, 875, 891, 1173
NlaIII CATG 7 cut(s) 776, 839, 850, 892, 953, 1260, 1351
NmuCI GTSAC 2 cut(s) 959, 1180
NsiI ATGCAT 1 cut(s) 1114
NspI RCATGY 2 cut(s) 776, 1351
OliI CACNNNNGTG 1 cut(s) 740
PagI TCATGA 2 cut(s) 888, 949
PciSI GCTCTTC 1 cut(s) 584
PctI GAATGC 1 cut(s) 1112
PdmI GAANNNNTTC 3 cut(s) 723, 862, 1051
PfeI GAWTC 3 cut(s) 593, 983, 996
PflMI CCANNNNNTGG 1 cut(s) 1334
PfoI TCCNGGA 1 cut(s) 1333
PkrI GCNGC 7 cut(s) 230, 339, 517, 784, 855, 922, 925
PleI GAGTC 2 cut(s) 299, 1187
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 2 cut(s) 299, 1187
Ppu21I YACGTR 1 cut(s) 740
Psp1406I AACGTT 1 cut(s) 723
Psp6I CCWGG 1 cut(s) 1333
PspCI CACGTG 1 cut(s) 740
PspFI CCCAGC 2 cut(s) 333, 1122
PspGI CCWGG 1 cut(s) 1333
PspPI GGNCC 2 cut(s) 912, 1196
PsrI GAACNNNNNNTAC 2 cut(s) 230, 262
PvuII CAGCTG 1 cut(s) 337
RsaI GTAC 1 cut(s) 808
RsaNI GTAC 1 cut(s) 807
RseI CAYNNNNRTG 1 cut(s) 740
SapI GCTCTTC 1 cut(s) 584
SaqAI TTAA 6 cut(s) 146, 170, 620, 639, 978, 1137
SatI GCNGC 7 cut(s) 229, 338, 516, 783, 854, 921, 924
Sau3AI GATC 6 cut(s) 6, 382, 707, 875, 891, 1173
Sau96I GGNCC 2 cut(s) 912, 1196
SchI GAGTC 2 cut(s) 300, 1188
ScrFI CCNGG 1 cut(s) 1335
SduI GDGCHC 1 cut(s) 535
SfaNI GCATC 3 cut(s) 67, 667, 941
SgrAI CRCCGGYG 1 cut(s) 266
SinI GGWCC 1 cut(s) 1196
SmiMI CAYNNNNRTG 1 cut(s) 740
SmlI CTYRAG 1 cut(s) 1073
SmoI CTYRAG 1 cut(s) 1073
SpeI ACTAGT 1 cut(s) 755
Sse9I AATT 6 cut(s) 117, 295, 649, 862, 975, 1338
SsiI CCGC 6 cut(s) 102, 229, 435, 515, 528, 924
SspMI CTAG 3 cut(s) 614, 756, 789
StyD4I CCNGG 1 cut(s) 1333
TaiI ACGT 2 cut(s) 726, 742
TaqI TCGA 7 cut(s) 87, 261, 282, 477, 710, 999, 1227
TasI AATT 6 cut(s) 117, 295, 649, 862, 975, 1338
TauI GCSGC 3 cut(s) 231, 518, 926
TfiI GAWTC 3 cut(s) 593, 983, 996
Tru1I TTAA 6 cut(s) 146, 170, 620, 639, 978, 1137
Tru9I TTAA 6 cut(s) 146, 170, 620, 639, 978, 1137
TscAI CASTG 2 cut(s) 702, 846
TseFI GTSAC 2 cut(s) 959, 1180
TseI GCWGC 4 cut(s) 337, 782, 853, 920
Tsp45I GTSAC 2 cut(s) 959, 1180
TspDTI ATGAA 3 cut(s) 305, 824, 875
TspGWI ACGGA 1 cut(s) 395
TspRI CASTG 2 cut(s) 702, 846
Van91I CCANNNNNTGG 1 cut(s) 1334
VpaK11BI GGWCC 1 cut(s) 1196
XagI CCTNNNNNAGG 1 cut(s) 977
XapI RAATTY 2 cut(s) 862, 1338
XbaI TCTAGA 1 cut(s) 613
XceI RCATGY 2 cut(s) 776, 1351
XmnI GAANNNNTTC 3 cut(s) 723, 862, 1051
XspI CTAG 3 cut(s) 614, 756, 789
Zsp2I ATGCAT 1 cut(s) 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.