Rroxscaffold_2G00122880

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005543
Physical Location & Seq
Reverse (-)
56299247 .. 56300863
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00122880.1

Sequence Viewer

Length: 1353 bp
ATGGAGATCACTATTATCTCCAGAGATACTGCGAAACCATCTTCCTCTTCACTTCAGCATCTAAAACCTCACAAACTTTCCCTCTTCGACCAGCTCACTCCCGCGACTTACGTCCCAGTTGTCTTGTTCTACCCCAACTCATCACTAAGTACCAATCTTTCCCAGACCTTAAACCACTTGAAGACCTCTTTATGCGAGACCCTCACTCTCTACTACCCATATTCCGGCCGCACCAAGAACAACCTATACATTTTTGATTTCGAGGCGGGTGTCCCATACCTCGAAGCCCGAGTCAATTGTCGCCTGCCTGAGTTTCATAAGCTTCAAGAAACCCAACTGCTAAACGACCTCCTTCCATTTCTTCCATTCCGGAAGGAAACGGATCACTCAGATCTGCTTCCCTTGCTTGCTTGTCAAATCAATGTTTTCGCTTGCGGTGGATTAGCAATCTCTGTCTCCTTATCTCACAAGATTTTCGATGGGGATACGGCAAACGCTTTTCTAAAAACTTGGGCTGCTGTTTTTCGCGGGGCTCGCGCCGACATTATACACCCTAATCTCTCCCAAGCCTCCCTGCTCTTCCCACCAAGAGATTCGTTGCCTCAGAACTATCTAGATTTAATGGATAGCTTGTGGTTTAAGGGAAGCAATTATGTTTCAAAGAGATTTGTGTTTGATGCCAAATCCATAGCCACACTGCGAGCAAGATCGACGAGCGAAAACGTTCCCAAGCCCACACGTGTGGAGATACTAACTAGTTTTCTCTGGAAACATGCAATGGCTGCTTCTAGGGCATTGTCATCCGGTACTTCACCAAAGACATCCATAGTCGTTCATGCAGTGAACATGAGGCCGCGAATGAATTCACAAATGGGATCGGGTGATGTCATGATCGGAAATCTTTTTTGGTGGGCCTTAGCTGCCGCCGACGCATCTGAGGCAGACGCTCATGACTTGTGTGACTTAGTGAACCTAATTAAGGAATCAATCAAAGGATTCGACAGTGAGTATTTGGATAGTTTGCGAGGTGAAGATGGTGGATTTGGAACCATTTCTGAGTTTTGTAATCAGCTTGAGGCTATGCTTTCCTCAGAAACACCAGATATCTATGCATTCACAAGCTGGGTTAGCTTTTTTAATCAGGTTGATTTTGGTTGGGGGAGGCCATTTTGGATCGGAGCCATGGGGAAAGTTGGTCCTGCATTTAGAAATCTTACTGTGTTCGTCGAGAGACAATGGGATAGGGGCATTGAAGCATGGGTGAACTTGGAGGAGAACCAAATGGCTCTGTTAGAGAAAGACCCCCAGTTTTTAGCATTTGCTTCTCCAAATCCTGGAATTTCAAGCATGTGA

Protein Analysis

450

Amino Acids

50.31

Weight (kDa)

5.83

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 437 1.7e-73 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1334
AccII CGCG 4 cut(s) 104, 528, 537, 856
AccIII TCCGGA 1 cut(s) 369
AciI CCGC 7 cut(s) 102, 229, 266, 435, 528, 854, 924
AclI AACGTT 1 cut(s) 723
AclWI GGATC 3 cut(s) 390, 883, 1181
AcoI YGGCCR 1 cut(s) 226
AcsI RAATTY 2 cut(s) 862, 1338
AcuI CTGAAG 1 cut(s) 38
AcvI CACGTG 1 cut(s) 740
AfaI GTAC 2 cut(s) 151, 808
AfiI CCNNNNNNNGG 3 cut(s) 224, 979, 1334
AflIII ACRYGT 2 cut(s) 737, 739
AgsI TTSAA 5 cut(s) 181, 326, 660, 1253, 1344
AhlI ACTAGT 1 cut(s) 755
AjnI CCWGG 1 cut(s) 1333
AjuI GAANNNNNNNTTGG 2 cut(s) 889, 921
AleI CACNNNNGTG 1 cut(s) 740
AluBI AGCT 7 cut(s) 94, 322, 630, 920, 1072, 1122, 1131
AluI AGCT 7 cut(s) 94, 322, 630, 920, 1072, 1122, 1131
Alw26I GTCTC 3 cut(s) 191, 460, 1225
AlwI GGATC 3 cut(s) 390, 883, 1181
Ama87I CYCGRG 1 cut(s) 288
Aor13HI TCCGGA 1 cut(s) 369
AoxI GGCC 4 cut(s) 226, 851, 912, 1163
ApeKI GCWGC 3 cut(s) 515, 782, 920
ApoI RAATTY 2 cut(s) 862, 1338
ArsI GACNNNNNNTTYG 2 cut(s) 276, 308
Asp700I GAANNNNTTC 3 cut(s) 723, 862, 1051
AspLEI GCGC 1 cut(s) 539
AspS9I GGNCC 2 cut(s) 912, 1196
AsuHPI GGTGA 4 cut(s) 804, 893, 1040, 1273
AvaI CYCGRG 1 cut(s) 288
AvaII GGWCC 1 cut(s) 1196
BanII GRGCYC 1 cut(s) 535
BbrPI CACGTG 1 cut(s) 740
BbsI GAAGAC 1 cut(s) 188
BbvI GCAGC 3 cut(s) 502, 769, 907
BccI CCATC 3 cut(s) 46, 473, 1028
BceAI ACGGC 1 cut(s) 504
BciT130I CCWGG 1 cut(s) 1335
BciVI GTATCC 1 cut(s) 478
BcoDI GTCTC 3 cut(s) 191, 460, 1225
BcuI ACTAGT 1 cut(s) 755
BfaI CTAG 3 cut(s) 614, 756, 789
BfuI GTATCC 1 cut(s) 478
BglII AGATCT 1 cut(s) 391
BisI GCNGC 6 cut(s) 229, 516, 783, 854, 921, 924
BlsI GCNGC 6 cut(s) 230, 517, 784, 855, 922, 925
Bme1390I CCNGG 1 cut(s) 1335
Bme18I GGWCC 1 cut(s) 1196
BmeT110I CYCGRG 1 cut(s) 288
BmgT120I GGNCC 2 cut(s) 912, 1196
BmiI GGNNCC 2 cut(s) 1048, 1180
BmrFI CCNGG 1 cut(s) 1335
BmrI ACTGGG 2 cut(s) 110, 1300
BmsI GCATC 3 cut(s) 67, 667, 941
BmuI ACTGGG 2 cut(s) 110, 1300
BoxI GACNNNNGTC 1 cut(s) 110
BpiI GAAGAC 1 cut(s) 188
BpmI CTGGAG 1 cut(s) 4
Bpu10I CCTNAGC 1 cut(s) 916
BpuEI CTTGAG 1 cut(s) 1094
BsaAI YACGTR 1 cut(s) 740
BsaI GGTCTC 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 1182
BsaWI WCCGGW 2 cut(s) 369, 803
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 979, 1334
Bse1I ACTGG 2 cut(s) 116, 1306
Bse3DI GCAATG 1 cut(s) 783
BseAI TCCGGA 1 cut(s) 369
BseBI CCWGG 1 cut(s) 1335
BseDI CCNNGG 1 cut(s) 1182
BseGI GGATG 2 cut(s) 800, 821
BseLI CCNNNNNNNGG 3 cut(s) 224, 979, 1334
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 6 cut(s) 300, 402, 617, 927, 1047, 1104
BseNI ACTGG 2 cut(s) 116, 1306
BseRI GAGGAG 1 cut(s) 1286
BseX3I CGGCCG 1 cut(s) 226
BseXI GCAGC 3 cut(s) 502, 769, 907
BseYI CCCAGC 1 cut(s) 1122
Bsh1236I CGCG 4 cut(s) 104, 528, 537, 856
Bsh1285I CGRYCG 1 cut(s) 229
BshFI GGCC 4 cut(s) 228, 853, 914, 1165
BsiEI CGRYCG 1 cut(s) 229
BsiHKCI CYCGRG 1 cut(s) 288
BsiSI CCGG 3 cut(s) 225, 370, 804
BslFI GGGAC 2 cut(s) 98, 257
BslI CCNNNNNNNGG 3 cut(s) 224, 979, 1334
BsmAI GTCTC 3 cut(s) 191, 460, 1225
BsmFI GGGAC 2 cut(s) 98, 257
BsmI GAATGC 1 cut(s) 1112
BsnI GGCC 4 cut(s) 228, 853, 914, 1165
Bso31I GGTCTC 1 cut(s) 191
BsoBI CYCGRG 1 cut(s) 288
Bsp1286I GDGCHC 1 cut(s) 535
Bsp13I TCCGGA 1 cut(s) 369
Bsp143I GATC 7 cut(s) 6, 382, 391, 707, 875, 891, 1173
Bsp19I CCATGG 1 cut(s) 1182
BspACI CCGC 7 cut(s) 102, 229, 266, 435, 528, 854, 924
BspANI GGCC 4 cut(s) 228, 853, 914, 1165
BspCNI CTCAG 6 cut(s) 301, 401, 616, 928, 1048, 1103
BspEI TCCGGA 1 cut(s) 369
BspFNI CGCG 4 cut(s) 104, 528, 537, 856
BspHI TCATGA 2 cut(s) 888, 949
BspLI GGNNCC 2 cut(s) 1048, 1180
BspPI GGATC 3 cut(s) 390, 883, 1181
BspQI GCTCTTC 1 cut(s) 584
BspTNI GGTCTC 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 783
BsrI ACTGG 2 cut(s) 116, 1306
BssECI CCNNGG 1 cut(s) 1182
BssMI GATC 7 cut(s) 6, 382, 391, 707, 875, 891, 1173
BssT1I CCWWGG 1 cut(s) 1182
Bst2UI CCWGG 1 cut(s) 1335
Bst4CI ACNGT 2 cut(s) 1004, 1219
Bst6I CTCTTC 3 cut(s) 52, 89, 584
BstAPI GCANNNNNTGC 1 cut(s) 782
BstBAI YACGTR 1 cut(s) 740
BstC8I GCNNGC 5 cut(s) 305, 408, 433, 535, 702
BstDEI CTNAG 9 cut(s) 146, 309, 388, 603, 916, 936, 964, 1056, 1090
BstDSI CCRYGG 1 cut(s) 1182
BstENI CCTNNNNNAGG 1 cut(s) 977
BstF5I GGATG 2 cut(s) 800, 821
BstFNI CGCG 4 cut(s) 104, 528, 537, 856
BstHHI GCGC 1 cut(s) 539
BstKTI GATC 7 cut(s) 9, 385, 394, 710, 878, 894, 1176
BstMAI GTCTC 3 cut(s) 191, 460, 1225
BstMBI GATC 7 cut(s) 6, 382, 391, 707, 875, 891, 1173
BstMCI CGRYCG 1 cut(s) 229
BstMWI GCNNNNNNNGC 8 cut(s) 403, 534, 782, 791, 920, 929, 938, 1128
BstNI CCWGG 1 cut(s) 1335
BstNSI RCATGY 2 cut(s) 776, 1351
BstPAI GACNNNNGTC 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 1333
BstUI CGCG 4 cut(s) 104, 528, 537, 856
BstV1I GCAGC 3 cut(s) 502, 769, 907
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 1 cut(s) 391
BstXI CCANNNNNNTGG 1 cut(s) 742
BstYI RGATCY 1 cut(s) 391
BstZI CGGCCG 1 cut(s) 226
BsuI GTATCC 1 cut(s) 478
BsuRI GGCC 4 cut(s) 228, 853, 914, 1165
BtgI CCRYGG 1 cut(s) 1182
BtsCI GGATG 2 cut(s) 800, 821
BtsI GCAGTG 2 cut(s) 695, 846
BtsIMutI CAGTG 3 cut(s) 695, 846, 1009
Cac8I GCNNGC 5 cut(s) 305, 408, 433, 535, 702
CciI TCATGA 2 cut(s) 888, 949
CfoI GCGC 1 cut(s) 539
Cfr13I GGNCC 2 cut(s) 912, 1196
CseI GACGC 2 cut(s) 938, 953
Csp6I GTAC 2 cut(s) 150, 807
CviAII CATG 8 cut(s) 773, 836, 847, 889, 950, 1183, 1257, 1348
CviQI GTAC 2 cut(s) 150, 807
DdeI CTNAG 9 cut(s) 146, 309, 388, 603, 916, 936, 964, 1056, 1090
DpnI GATC 7 cut(s) 8, 384, 393, 709, 877, 893, 1175
DpnII GATC 7 cut(s) 6, 382, 391, 707, 875, 891, 1173
EaeI YGGCCR 1 cut(s) 226
EagI CGGCCG 1 cut(s) 226
Eam1104I CTCTTC 3 cut(s) 52, 89, 584
EarI CTCTTC 3 cut(s) 52, 89, 584
EclXI CGGCCG 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 1182
Eco24I GRGCYC 1 cut(s) 535
Eco31I GGTCTC 1 cut(s) 191
Eco32I GATATC 1 cut(s) 1105
Eco47I GGWCC 1 cut(s) 1196
Eco52I CGGCCG 1 cut(s) 226
Eco57I CTGAAG 1 cut(s) 38
Eco72I CACGTG 1 cut(s) 740
Eco88I CYCGRG 1 cut(s) 288
EcoNI CCTNNNNNAGG 1 cut(s) 977
EcoRI GAATTC 1 cut(s) 862
EcoRII CCWGG 1 cut(s) 1333
EcoRV GATATC 1 cut(s) 1105
EcoT14I CCWWGG 1 cut(s) 1182
EcoT22I ATGCAT 1 cut(s) 1114
EcoT38I GRGCYC 1 cut(s) 535
ErhI CCWWGG 1 cut(s) 1182
FaeI CATG 8 cut(s) 776, 839, 850, 892, 953, 1186, 1260, 1351
FaqI GGGAC 2 cut(s) 98, 257
FatI CATG 8 cut(s) 772, 835, 846, 888, 949, 1182, 1256, 1347
FauI CCCGC 3 cut(s) 109, 259, 521
Fnu4HI GCNGC 6 cut(s) 229, 516, 783, 854, 921, 924
FokI GGATG 2 cut(s) 787, 808
FriOI GRGCYC 1 cut(s) 535
Fsp4HI GCNGC 6 cut(s) 229, 516, 783, 854, 921, 924
FspBI CTAG 3 cut(s) 614, 756, 789
GlaI GCGC 1 cut(s) 538
GluI GCNGC 6 cut(s) 229, 516, 783, 854, 921, 924
GsaI CCCAGC 1 cut(s) 1126
GsuI CTGGAG 1 cut(s) 4
HaeIII GGCC 4 cut(s) 228, 853, 914, 1165
HapII CCGG 3 cut(s) 225, 370, 804
HgaI GACGC 2 cut(s) 938, 953
HhaI GCGC 1 cut(s) 539
Hin1II CATG 8 cut(s) 776, 839, 850, 892, 953, 1186, 1260, 1351
Hin6I GCGC 1 cut(s) 537
HinP1I GCGC 1 cut(s) 537
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 4 cut(s) 291, 593, 983, 996
HpaII CCGG 3 cut(s) 225, 370, 804
HphI GGTGA 4 cut(s) 804, 893, 1040, 1273
Hpy166II GTNNAC 3 cut(s) 844, 970, 1264
Hpy188I TCNGA 7 cut(s) 391, 606, 896, 937, 1057, 1093, 1178
Hpy188III TCNNGA 8 cut(s) 21, 326, 370, 614, 766, 889, 950, 1228
Hpy8I GTNNAC 3 cut(s) 844, 970, 1264
Hpy99I CGWCG 3 cut(s) 715, 932, 1229
HpyAV CCTTC 2 cut(s) 362, 367
HpyCH4III ACNGT 2 cut(s) 1004, 1219
HpyCH4IV ACGT 3 cut(s) 111, 723, 739
HpyCH4V TGCA 4 cut(s) 776, 839, 1112, 1202
HpyF10VI GCNNNNNNNGC 8 cut(s) 403, 534, 782, 791, 920, 929, 938, 1128
HpyF3I CTNAG 9 cut(s) 146, 309, 388, 603, 916, 936, 964, 1056, 1090
HpySE526I ACGT 3 cut(s) 111, 723, 739
Hsp92II CATG 8 cut(s) 776, 839, 850, 892, 953, 1186, 1260, 1351
HspAI GCGC 1 cut(s) 537
Kpn2I TCCGGA 1 cut(s) 369
Kzo9I GATC 7 cut(s) 6, 382, 391, 707, 875, 891, 1173
LguI GCTCTTC 1 cut(s) 584
LmnI GCTCC 1 cut(s) 1178
Lsp1109I GCAGC 3 cut(s) 502, 769, 907
LweI GCATC 3 cut(s) 67, 667, 941
MaeI CTAG 3 cut(s) 614, 756, 789
MaeII ACGT 3 cut(s) 111, 723, 739
MaeIII GTNAC 1 cut(s) 959
MalI GATC 7 cut(s) 8, 384, 393, 709, 877, 893, 1175
MboI GATC 7 cut(s) 6, 382, 391, 707, 875, 891, 1173
MboII GAAGA 7 cut(s) 33, 39, 76, 193, 353, 571, 1043
MfeI CAATTG 1 cut(s) 295
MflI RGATCY 1 cut(s) 391
MhlI GDGCHC 1 cut(s) 535
MluCI AATT 5 cut(s) 295, 649, 862, 975, 1338
MlyI GAGTC 1 cut(s) 300
Mph1103I ATGCAT 1 cut(s) 1114
MroI TCCGGA 1 cut(s) 369
MroXI GAANNNNTTC 3 cut(s) 723, 862, 1051
MseI TTAA 5 cut(s) 170, 620, 639, 978, 1137
MslI CAYNNNNRTG 1 cut(s) 740
MspI CCGG 3 cut(s) 225, 370, 804
MspR9I CCNGG 1 cut(s) 1335
MunI CAATTG 1 cut(s) 295
Mva1269I GAATGC 1 cut(s) 1112
MvaI CCWGG 1 cut(s) 1335
MvnI CGCG 4 cut(s) 104, 528, 537, 856
MwoI GCNNNNNNNGC 8 cut(s) 403, 534, 782, 791, 920, 929, 938, 1128
NcoI CCATGG 1 cut(s) 1182
NdeII GATC 7 cut(s) 6, 382, 391, 707, 875, 891, 1173
NlaIII CATG 8 cut(s) 776, 839, 850, 892, 953, 1186, 1260, 1351
NlaIV GGNNCC 2 cut(s) 1048, 1180
NmuCI GTSAC 1 cut(s) 959
NsiI ATGCAT 1 cut(s) 1114
NspI RCATGY 2 cut(s) 776, 1351
OliI CACNNNNGTG 1 cut(s) 740
PagI TCATGA 2 cut(s) 888, 949
PciSI GCTCTTC 1 cut(s) 584
PctI GAATGC 1 cut(s) 1112
PdmI GAANNNNTTC 3 cut(s) 723, 862, 1051
PfeI GAWTC 3 cut(s) 593, 983, 996
PflMI CCANNNNNTGG 1 cut(s) 1334
PfoI TCCNGGA 1 cut(s) 1333
PkrI GCNGC 6 cut(s) 230, 517, 784, 855, 922, 925
PleI GAGTC 1 cut(s) 299
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 1 cut(s) 299
Ppu21I YACGTR 1 cut(s) 740
PshAI GACNNNNGTC 1 cut(s) 110
Psp1406I AACGTT 1 cut(s) 723
Psp6I CCWGG 1 cut(s) 1333
PspCI CACGTG 1 cut(s) 740
PspFI CCCAGC 1 cut(s) 1122
PspGI CCWGG 1 cut(s) 1333
PspN4I GGNNCC 2 cut(s) 1048, 1180
PspPI GGNCC 2 cut(s) 912, 1196
PsrI GAACNNNNNNTAC 2 cut(s) 230, 262
PsuI RGATCY 1 cut(s) 391
RsaI GTAC 2 cut(s) 151, 808
RsaNI GTAC 2 cut(s) 150, 807
RseI CAYNNNNRTG 1 cut(s) 740
SapI GCTCTTC 1 cut(s) 584
SaqAI TTAA 5 cut(s) 170, 620, 639, 978, 1137
SatI GCNGC 6 cut(s) 229, 516, 783, 854, 921, 924
Sau3AI GATC 7 cut(s) 6, 382, 391, 707, 875, 891, 1173
Sau96I GGNCC 2 cut(s) 912, 1196
SchI GAGTC 1 cut(s) 300
ScrFI CCNGG 1 cut(s) 1335
SduI GDGCHC 1 cut(s) 535
SfaNI GCATC 3 cut(s) 67, 667, 941
SinI GGWCC 1 cut(s) 1196
SmiMI CAYNNNNRTG 1 cut(s) 740
SmlI CTYRAG 1 cut(s) 1073
SmoI CTYRAG 1 cut(s) 1073
SpeI ACTAGT 1 cut(s) 755
Sse9I AATT 5 cut(s) 295, 649, 862, 975, 1338
SsiI CCGC 7 cut(s) 102, 229, 266, 435, 528, 854, 924
SspMI CTAG 3 cut(s) 614, 756, 789
StyD4I CCNGG 1 cut(s) 1333
StyI CCWWGG 1 cut(s) 1182
TaaI ACNGT 2 cut(s) 1004, 1219
TaiI ACGT 3 cut(s) 114, 726, 742
TaqI TCGA 7 cut(s) 87, 261, 282, 477, 710, 999, 1227
TasI AATT 5 cut(s) 295, 649, 862, 975, 1338
TauI GCSGC 3 cut(s) 231, 856, 926
TfiI GAWTC 3 cut(s) 593, 983, 996
Tru1I TTAA 5 cut(s) 170, 620, 639, 978, 1137
Tru9I TTAA 5 cut(s) 170, 620, 639, 978, 1137
TscAI CASTG 3 cut(s) 702, 846, 1009
TseFI GTSAC 1 cut(s) 959
TseI GCWGC 3 cut(s) 515, 782, 920
Tsp45I GTSAC 1 cut(s) 959
TspDTI ATGAA 3 cut(s) 305, 824, 875
TspGWI ACGGA 1 cut(s) 395
TspRI CASTG 3 cut(s) 702, 846, 1009
Van91I CCANNNNNTGG 1 cut(s) 1334
VpaK11BI GGWCC 1 cut(s) 1196
XagI CCTNNNNNAGG 1 cut(s) 977
XapI RAATTY 2 cut(s) 862, 1338
XbaI TCTAGA 1 cut(s) 613
XceI RCATGY 2 cut(s) 776, 1351
XmnI GAANNNNTTC 3 cut(s) 723, 862, 1051
XspI CTAG 3 cut(s) 614, 756, 789
Zsp2I ATGCAT 1 cut(s) 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.