Rh4DG187800

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0005543
Physical Location & Seq
Forward (+)
37027125 .. 37027744
620 bp
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UTR
Exon/CDS
Intron
Rh4DG187800.1

Sequence Viewer

Length: 453 bp
ATGCCCAAGCCATCACGTAATGACGCACTCACATGTTTTATTTGGAAACATGCAATGGCTTCTTCTCAGGTTCTGTTACCAGGTTCCTCACCAAGAACTTCCATAGTAGCGCATGCAATGAACATGAGATCACGGATGAAGCCACAAACGTCGTCAGCTAATATCATAGGAAATTTCTTTTGGTGGGTGGCAACAGTCGCCGACTCATCTAAGCTAGCAGAAGATCATGAGTTATGTGACTTGGTTCACCTGATTAATGAATCTCACAAAGGATTTGATAGGGGTTATTTTGAGTCTCATCAGGGAGAAGAGGGATTTGGAGCCATTGGAAACTTCTTTTATCAACTAGAAGTCTTGCTTTCTTCAAAACCCCCACCAGAACTTTATTTATTCACCAATTGGACAAGCATTTTTCACAAATTGGATTTTGGTGGGGGAAGCCTTTCTGGGTAG

Protein Analysis

150

Amino Acids

16.72

Weight (kDa)

6.36

Isoelectric Point (pI)

41.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 3 - 147 2.8e-08 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 172
AflIII ACRYGT 1 cut(s) 32
AgsI TTSAA 1 cut(s) 366
AjnI CCWGG 1 cut(s) 79
AjuI GAANNNNNNNTTGG 4 cut(s) 163, 195, 300, 332
AluBI AGCT 2 cut(s) 158, 214
AluI AGCT 2 cut(s) 158, 214
Alw26I GTCTC 1 cut(s) 300
AlwNI CAGNNNCTG 1 cut(s) 73
ApoI RAATTY 1 cut(s) 172
AseI ATTAAT 1 cut(s) 255
Asp700I GAANNNNTTC 1 cut(s) 442
AspLEI GCGC 1 cut(s) 112
AsuHPI GGTGA 3 cut(s) 81, 239, 385
AsuNHI GCTAGC 1 cut(s) 214
BccI CCATC 1 cut(s) 19
BciT130I CCWGG 1 cut(s) 81
BcoDI GTCTC 1 cut(s) 300
BfaI CTAG 2 cut(s) 215, 347
Bme1390I CCNGG 1 cut(s) 81
BmiI GGNNCC 2 cut(s) 85, 322
BmrFI CCNGG 1 cut(s) 81
BmtI GCTAGC 1 cut(s) 218
BsaAI YACGTR 1 cut(s) 17
Bse3DI GCAATG 2 cut(s) 60, 123
BseBI CCWGG 1 cut(s) 81
BseGI GGATG 1 cut(s) 141
BseMI GCAATG 2 cut(s) 60, 123
BseMII CTCAG 1 cut(s) 80
BsmAI GTCTC 1 cut(s) 300
Bsp143I GATC 2 cut(s) 128, 223
BspCNI CTCAG 1 cut(s) 79
BspHI TCATGA 1 cut(s) 226
BspLI GGNNCC 2 cut(s) 85, 322
BspOI GCTAGC 1 cut(s) 218
BsrDI GCAATG 2 cut(s) 60, 123
BssMI GATC 2 cut(s) 128, 223
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 196
Bst6I CTCTTC 1 cut(s) 303
BstBAI YACGTR 1 cut(s) 17
BstC8I GCNNGC 2 cut(s) 114, 216
BstDEI CTNAG 2 cut(s) 66, 210
BstF5I GGATG 1 cut(s) 141
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 2 cut(s) 131, 226
BstMAI GTCTC 1 cut(s) 300
BstMBI GATC 2 cut(s) 128, 223
BstMWI GCNNNNNNNGC 1 cut(s) 197
BstNI CCWGG 1 cut(s) 81
BstNSI RCATGY 3 cut(s) 36, 53, 116
BstSCI CCNGG 1 cut(s) 79
BtsCI GGATG 1 cut(s) 141
Cac8I GCNNGC 2 cut(s) 114, 216
CaiI CAGNNNCTG 1 cut(s) 73
CciI TCATGA 1 cut(s) 226
CfoI GCGC 1 cut(s) 112
CseI GACGC 1 cut(s) 32
CsiI ACCWGGT 1 cut(s) 79
CviAII CATG 5 cut(s) 33, 50, 113, 124, 227
CviJI RGCY 7 cut(s) 10, 59, 142, 158, 214, 323, 441
CviKI_1 RGCY 7 cut(s) 10, 59, 142, 158, 214, 323, 441
DdeI CTNAG 2 cut(s) 66, 210
DpnI GATC 2 cut(s) 130, 225
DpnII GATC 2 cut(s) 128, 223
Eam1104I CTCTTC 1 cut(s) 303
EarI CTCTTC 1 cut(s) 303
EcoRII CCWGG 1 cut(s) 79
FaeI CATG 5 cut(s) 36, 53, 116, 127, 230
FaiI YATR 8 cut(s) 34, 51, 104, 114, 125, 167, 228, 235
FalI AAGNNNNNCTT 2 cut(s) 342, 374
FatI CATG 5 cut(s) 32, 49, 112, 123, 226
FokI GGATG 1 cut(s) 148
FspBI CTAG 2 cut(s) 215, 347
GlaI GCGC 1 cut(s) 111
HgaI GACGC 1 cut(s) 32
HhaI GCGC 1 cut(s) 112
Hin1II CATG 5 cut(s) 36, 53, 116, 127, 230
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HinfI GANTC 3 cut(s) 203, 260, 293
HphI GGTGA 3 cut(s) 81, 239, 385
Hpy166II GTNNAC 1 cut(s) 247
Hpy188III TCNNGA 1 cut(s) 227
Hpy8I GTNNAC 1 cut(s) 247
Hpy99I CGWCG 1 cut(s) 154
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4IV ACGT 2 cut(s) 16, 149
HpyCH4V TGCA 2 cut(s) 53, 116
HpyF10VI GCNNNNNNNGC 1 cut(s) 197
HpyF3I CTNAG 2 cut(s) 66, 210
HpySE526I ACGT 2 cut(s) 16, 149
Hsp92II CATG 5 cut(s) 36, 53, 116, 127, 230
HspAI GCGC 1 cut(s) 110
Kzo9I GATC 2 cut(s) 128, 223
LmnI GCTCC 1 cut(s) 320
LpnPI CCDG 7 cut(s) 53, 66, 93, 263, 287, 390, 432
MabI ACCWGGT 1 cut(s) 79
MaeI CTAG 2 cut(s) 215, 347
MaeII ACGT 2 cut(s) 16, 149
MaeIII GTNAC 2 cut(s) 75, 236
MalI GATC 2 cut(s) 130, 225
MboI GATC 2 cut(s) 128, 223
MboII GAAGA 4 cut(s) 54, 233, 320, 354
MfeI CAATTG 1 cut(s) 397
MluCI AATT 3 cut(s) 172, 397, 419
MlyI GAGTC 2 cut(s) 197, 302
MnlI CCTC 2 cut(s) 97, 304
MroXI GAANNNNTTC 1 cut(s) 442
MseI TTAA 1 cut(s) 255
MslI CAYNNNNRTG 1 cut(s) 31
MspR9I CCNGG 1 cut(s) 81
MunI CAATTG 1 cut(s) 397
MvaI CCWGG 1 cut(s) 81
MwoI GCNNNNNNNGC 1 cut(s) 197
NdeII GATC 2 cut(s) 128, 223
NheI GCTAGC 1 cut(s) 214
NlaIII CATG 5 cut(s) 36, 53, 116, 127, 230
NlaIV GGNNCC 2 cut(s) 85, 322
NmuCI GTSAC 1 cut(s) 236
NspI RCATGY 3 cut(s) 36, 53, 116
PaeI GCATGC 1 cut(s) 116
PagI TCATGA 1 cut(s) 226
PciI ACATGT 1 cut(s) 32
PdmI GAANNNNTTC 1 cut(s) 442
PfeI GAWTC 1 cut(s) 260
PleI GAGTC 2 cut(s) 197, 301
PpsI GAGTC 2 cut(s) 197, 301
Ppu21I YACGTR 1 cut(s) 17
PscI ACATGT 1 cut(s) 32
PshBI ATTAAT 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 79
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 2 cut(s) 85, 322
PstNI CAGNNNCTG 1 cut(s) 73
RseI CAYNNNNRTG 1 cut(s) 31
SaqAI TTAA 1 cut(s) 255
Sau3AI GATC 2 cut(s) 128, 223
SchI GAGTC 2 cut(s) 197, 302
ScrFI CCNGG 1 cut(s) 81
SetI ASST 7 cut(s) 19, 72, 85, 152, 160, 216, 252
SexAI ACCWGGT 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 31
SphI GCATGC 1 cut(s) 116
Sse9I AATT 3 cut(s) 172, 397, 419
SspMI CTAG 2 cut(s) 215, 347
StyD4I CCNGG 1 cut(s) 79
TaaI ACNGT 1 cut(s) 196
TaiI ACGT 2 cut(s) 19, 152
TasI AATT 3 cut(s) 172, 397, 419
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TseFI GTSAC 1 cut(s) 236
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 3 cut(s) 134, 152, 273
TspGWI ACGGA 1 cut(s) 148
VspI ATTAAT 1 cut(s) 255
XapI RAATTY 1 cut(s) 172
XceI RCATGY 3 cut(s) 36, 53, 116
XmnI GAANNNNTTC 1 cut(s) 442
XspI CTAG 2 cut(s) 215, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.