Rw1G027250

vinorine synthase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005543
Physical Location & Seq
Forward (+)
54684345 .. 54686461
2117 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G027250.1

Sequence Viewer

Length: 1353 bp
ATGGAGATCACTATTATCTCCAGATATACTGCGAAACCATCTTCTTCTTCACTTCGGCATCTAAAACCTCACAAACTTTCCCTCTTCGACCAGCTCACTCCCGCAACTTACGCCCCAATTGTCTTGTTCTACCCCAACTCATCATTAACTACCAATCTTTCCCAAACCTTAGTCCACTTGAAGACCTCTCTATGCGAGACCCTCACTCTCTACTACCCATTTTCCGGCCGCATCAAGAACAACCTCTACATTTATGATTTCGACGCGGGTGTTCCATACCTCGAAGCTCGAGTCAATTGTCGCCTGCCTGAGTTTCTTAAGCTTCAAGAAACCCAGCTGCTAAACGACCTCCTTCCAGTTCTTCCATTCCGGAAGGAAACCGATCACTCAGATCTGCTTCCCTTGCTTGCTTGTCAAATCAATGTTTTCGCTTGTGGTGGATTGGCAATCTCTGCCTCCGTGTCTCACAAGATTTTCGATGGGGATACGGGAAACGCTTTTCTCAAAACTTGGGCTTCCATTTTTCGCGGGGCTCGCGACGACATTGTACACCCTAATCTCTCCGAAGCCTCCCTGTTCTTCCCACCAAGAGATCCGTTGCCTCAGAACTATCTAGATTTAATGGATAGCTTGTGGTTTAAGAGAAGCAATTATATTTCAAAGAGATTTGTGTTTGATGCCAAATCCATAGCCACACTGCGAGCAAGATCGACGAGCGAAAACGTTCCCAAGCCCACACGTGTGGAGATACTAACTAGTTTTATCTGGAAACATGCAATGGCTGCTTCTAGGGCACTGTCATCCGGTACTTCACCAAAGACATCCATAGTCGTTCATGCAGTGAACATGAGACCGCGAATGAATTCACAAATGCGATCGGATACTGTCATGATCGGAAATCTTTTTTGGTGGGCCGTAGCTGCCGCCGACGCTTCTGAGGCAGACGCTCATGACTTGCGTGACTTAGTGAACATAATTAAGGAATCAATCAAGGGATTCGATGGTGAGTATTTGGAGAGTTTGCAAGGTGAAGATGGTGGATTCGGAACCATTTCTGAGTTTTGTATTCAGCTTGAGGCTATGCTTTCCTCAGAAACACCGGATATCTATGCATTCACAAGCTGGGCTAGCGTTTTAAAACAGGTTGATTTTGGTTGGGGGAGGCCATTTTGGATCGGAGCCATGGGGAAAGTTGGTCCTGCATTTAGAAATCTCACTGTGTTCGTCGACAGACAATGGGATAGGGGCATTGAAGCATGGGTGAACTTGGAGGAAAACCAAATGGCTCTGTTAGAGAAAGACCCCCAGTTTTTAGCATTTGCTTCTCCAAATCCTGGAATATCAAGCATGTAA

Protein Analysis

450

Amino Acids

50.49

Weight (kDa)

6.17

Isoelectric Point (pI)

38.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 1 - 437 1.6e-75 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1334
AccI GTMKAC 1 cut(s) 1227
AccII CGCG 4 cut(s) 266, 528, 537, 856
AccIII TCCGGA 1 cut(s) 369
AciI CCGC 6 cut(s) 102, 229, 266, 528, 854, 924
AclI AACGTT 1 cut(s) 723
AclWI GGATC 2 cut(s) 587, 1181
AcoI YGGCCR 1 cut(s) 226
AcsI RAATTY 1 cut(s) 862
AcvI CACGTG 1 cut(s) 740
AfaI GTAC 2 cut(s) 549, 808
AfiI CCNNNNNNNGG 2 cut(s) 224, 1334
AflII CTTAAG 1 cut(s) 317
AflIII ACRYGT 2 cut(s) 737, 739
AgsI TTSAA 4 cut(s) 181, 326, 660, 1253
AhlI ACTAGT 1 cut(s) 755
AjnI CCWGG 1 cut(s) 1333
AjuI GAANNNNNNNTTGG 2 cut(s) 889, 921
AleI CACNNNNGTG 1 cut(s) 740
AluBI AGCT 8 cut(s) 94, 287, 322, 337, 630, 920, 1072, 1122
AluI AGCT 8 cut(s) 94, 287, 322, 337, 630, 920, 1072, 1122
Alw26I GTCTC 3 cut(s) 191, 468, 844
AlwI GGATC 2 cut(s) 587, 1181
Ama87I CYCGRG 1 cut(s) 288
Aor13HI TCCGGA 1 cut(s) 369
AoxI GGCC 3 cut(s) 226, 912, 1163
ApeKI GCWGC 3 cut(s) 337, 782, 920
ApoI RAATTY 1 cut(s) 862
ArsI GACNNNNNNTTYG 2 cut(s) 276, 308
Asp700I GAANNNNTTC 3 cut(s) 723, 862, 1051
AspS9I GGNCC 2 cut(s) 912, 1196
AsuHPI GGTGA 4 cut(s) 804, 1016, 1040, 1273
AsuNHI GCTAGC 1 cut(s) 1127
AvaI CYCGRG 1 cut(s) 288
AvaII GGWCC 1 cut(s) 1196
BaeGI GKGCMC 1 cut(s) 796
BanII GRGCYC 1 cut(s) 535
BbrPI CACGTG 1 cut(s) 740
BbsI GAAGAC 1 cut(s) 188
BbvI GCAGC 3 cut(s) 324, 769, 907
BccI CCATC 4 cut(s) 46, 473, 995, 1028
BceAI ACGGC 1 cut(s) 899
BciT130I CCWGG 1 cut(s) 1335
BciVI GTATCC 2 cut(s) 478, 874
BcoDI GTCTC 3 cut(s) 191, 468, 844
BcuI ACTAGT 1 cut(s) 755
BfaI CTAG 4 cut(s) 614, 756, 789, 1128
BfrI CTTAAG 1 cut(s) 317
BfuI GTATCC 2 cut(s) 478, 874
BglII AGATCT 1 cut(s) 391
BisI GCNGC 5 cut(s) 229, 338, 783, 921, 924
BlsI GCNGC 5 cut(s) 230, 339, 784, 922, 925
Bme1390I CCNGG 1 cut(s) 1335
Bme18I GGWCC 1 cut(s) 1196
BmeT110I CYCGRG 1 cut(s) 288
BmgT120I GGNCC 2 cut(s) 912, 1196
BmiI GGNNCC 2 cut(s) 1048, 1180
BmrFI CCNGG 1 cut(s) 1335
BmrI ACTGGG 1 cut(s) 1300
BmsI GCATC 3 cut(s) 67, 240, 667
BmtI GCTAGC 1 cut(s) 1131
BmuI ACTGGG 1 cut(s) 1300
BpiI GAAGAC 1 cut(s) 188
BpmI CTGGAG 1 cut(s) 4
BpuEI CTTGAG 1 cut(s) 1094
BsaAI YACGTR 1 cut(s) 740
BsaI GGTCTC 2 cut(s) 191, 844
BsaJI CCNNGG 1 cut(s) 1182
BsaWI WCCGGW 3 cut(s) 369, 803, 1099
Bsc4I CCNNNNNNNGG 2 cut(s) 224, 1334
Bse1I ACTGG 2 cut(s) 356, 1306
Bse3DI GCAATG 1 cut(s) 783
BseAI TCCGGA 1 cut(s) 369
BseBI CCWGG 1 cut(s) 1335
BseDI CCNNGG 1 cut(s) 1182
BseGI GGATG 2 cut(s) 800, 821
BseLI CCNNNNNNNGG 2 cut(s) 224, 1334
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 6 cut(s) 300, 402, 617, 927, 1047, 1104
BseNI ACTGG 2 cut(s) 356, 1306
BseSI GKGCMC 1 cut(s) 796
BseX3I CGGCCG 1 cut(s) 226
BseXI GCAGC 3 cut(s) 324, 769, 907
BseYI CCCAGC 2 cut(s) 333, 1122
Bsh1236I CGCG 4 cut(s) 266, 528, 537, 856
Bsh1285I CGRYCG 2 cut(s) 229, 878
BshFI GGCC 3 cut(s) 228, 914, 1165
BsiEI CGRYCG 2 cut(s) 229, 878
BsiHKCI CYCGRG 1 cut(s) 288
BsiSI CCGG 4 cut(s) 225, 370, 804, 1100
BslI CCNNNNNNNGG 2 cut(s) 224, 1334
BsmAI GTCTC 3 cut(s) 191, 468, 844
BsmI GAATGC 1 cut(s) 1112
BsnI GGCC 3 cut(s) 228, 914, 1165
Bso31I GGTCTC 2 cut(s) 191, 844
BsoBI CYCGRG 1 cut(s) 288
Bsp1286I GDGCHC 2 cut(s) 535, 796
Bsp13I TCCGGA 1 cut(s) 369
Bsp1407I TGTACA 1 cut(s) 547
Bsp143I GATC 8 cut(s) 6, 382, 391, 592, 707, 875, 891, 1173
Bsp19I CCATGG 1 cut(s) 1182
Bsp68I TCGCGA 1 cut(s) 537
BspACI CCGC 6 cut(s) 102, 229, 266, 528, 854, 924
BspANI GGCC 3 cut(s) 228, 914, 1165
BspCNI CTCAG 6 cut(s) 301, 401, 616, 928, 1048, 1103
BspEI TCCGGA 1 cut(s) 369
BspFNI CGCG 4 cut(s) 266, 528, 537, 856
BspHI TCATGA 2 cut(s) 888, 949
BspLI GGNNCC 2 cut(s) 1048, 1180
BspOI GCTAGC 1 cut(s) 1131
BspPI GGATC 2 cut(s) 587, 1181
BspTI CTTAAG 1 cut(s) 317
BspTNI GGTCTC 2 cut(s) 191, 844
BsrDI GCAATG 1 cut(s) 783
BsrGI TGTACA 1 cut(s) 547
BsrI ACTGG 2 cut(s) 356, 1306
BssECI CCNNGG 1 cut(s) 1182
BssMI GATC 8 cut(s) 6, 382, 391, 592, 707, 875, 891, 1173
BssT1I CCWWGG 1 cut(s) 1182
Bst2UI CCWGG 1 cut(s) 1335
Bst4CI ACNGT 3 cut(s) 798, 886, 1219
Bst6I CTCTTC 1 cut(s) 89
BstAFI CTTAAG 1 cut(s) 317
BstAPI GCANNNNNTGC 2 cut(s) 452, 782
BstAUI TGTACA 1 cut(s) 547
BstBAI YACGTR 1 cut(s) 740
BstC8I GCNNGC 5 cut(s) 305, 408, 535, 702, 1129
BstDEI CTNAG 8 cut(s) 169, 309, 388, 603, 936, 964, 1056, 1090
BstDSI CCRYGG 1 cut(s) 1182
BstF5I GGATG 2 cut(s) 800, 821
BstFNI CGCG 4 cut(s) 266, 528, 537, 856
BstKTI GATC 8 cut(s) 9, 385, 394, 595, 710, 878, 894, 1176
BstMAI GTCTC 3 cut(s) 191, 468, 844
BstMBI GATC 8 cut(s) 6, 382, 391, 592, 707, 875, 891, 1173
BstMCI CGRYCG 2 cut(s) 229, 878
BstNI CCWGG 1 cut(s) 1335
BstNSI RCATGY 2 cut(s) 776, 1351
BstSCI CCNGG 1 cut(s) 1333
BstSLI GKGCMC 1 cut(s) 796
BstUI CGCG 4 cut(s) 266, 528, 537, 856
BstV1I GCAGC 3 cut(s) 324, 769, 907
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 2 cut(s) 391, 592
BstXI CCANNNNNNTGG 1 cut(s) 742
BstYI RGATCY 2 cut(s) 391, 592
BstZI CGGCCG 1 cut(s) 226
BsuI GTATCC 2 cut(s) 478, 874
BsuRI GGCC 3 cut(s) 228, 914, 1165
BtgI CCRYGG 1 cut(s) 1182
BtsCI GGATG 2 cut(s) 800, 821
BtsI GCAGTG 2 cut(s) 695, 846
BtsIMutI CAGTG 4 cut(s) 695, 794, 846, 1215
BtuMI TCGCGA 1 cut(s) 537
Cac8I GCNNGC 5 cut(s) 305, 408, 535, 702, 1129
CciI TCATGA 2 cut(s) 888, 949
Cfr13I GGNCC 2 cut(s) 912, 1196
CseI GACGC 3 cut(s) 272, 938, 953
Csp6I GTAC 2 cut(s) 548, 807
CviAII CATG 8 cut(s) 773, 836, 847, 889, 950, 1183, 1257, 1348
CviQI GTAC 2 cut(s) 548, 807
DdeI CTNAG 8 cut(s) 169, 309, 388, 603, 936, 964, 1056, 1090
DpnI GATC 8 cut(s) 8, 384, 393, 594, 709, 877, 893, 1175
DpnII GATC 8 cut(s) 6, 382, 391, 592, 707, 875, 891, 1173
DraI TTTAAA 1 cut(s) 1137
EaeI YGGCCR 1 cut(s) 226
EagI CGGCCG 1 cut(s) 226
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
EclXI CGGCCG 1 cut(s) 226
Eco130I CCWWGG 1 cut(s) 1182
Eco24I GRGCYC 1 cut(s) 535
Eco31I GGTCTC 2 cut(s) 191, 844
Eco32I GATATC 1 cut(s) 1105
Eco47I GGWCC 1 cut(s) 1196
Eco52I CGGCCG 1 cut(s) 226
Eco72I CACGTG 1 cut(s) 740
Eco88I CYCGRG 1 cut(s) 288
EcoRI GAATTC 1 cut(s) 862
EcoRII CCWGG 1 cut(s) 1333
EcoRV GATATC 1 cut(s) 1105
EcoT14I CCWWGG 1 cut(s) 1182
EcoT22I ATGCAT 1 cut(s) 1114
EcoT38I GRGCYC 1 cut(s) 535
ErhI CCWWGG 1 cut(s) 1182
FaeI CATG 8 cut(s) 776, 839, 850, 892, 953, 1186, 1260, 1351
FatI CATG 8 cut(s) 772, 835, 846, 888, 949, 1182, 1256, 1347
FauI CCCGC 3 cut(s) 109, 259, 521
FblI GTMKAC 1 cut(s) 1227
Fnu4HI GCNGC 5 cut(s) 229, 338, 783, 921, 924
FokI GGATG 2 cut(s) 787, 808
FriOI GRGCYC 1 cut(s) 535
Fsp4HI GCNGC 5 cut(s) 229, 338, 783, 921, 924
FspBI CTAG 4 cut(s) 614, 756, 789, 1128
GluI GCNGC 5 cut(s) 229, 338, 783, 921, 924
GsaI CCCAGC 2 cut(s) 337, 1126
GsuI CTGGAG 1 cut(s) 4
HaeIII GGCC 3 cut(s) 228, 914, 1165
HapII CCGG 4 cut(s) 225, 370, 804, 1100
HgaI GACGC 3 cut(s) 272, 938, 953
Hin1II CATG 8 cut(s) 776, 839, 850, 892, 953, 1186, 1260, 1351
HincII GTYRAC 1 cut(s) 1228
HindII GTYRAC 1 cut(s) 1228
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 4 cut(s) 291, 983, 996, 1041
HpaII CCGG 4 cut(s) 225, 370, 804, 1100
HphI GGTGA 4 cut(s) 804, 1016, 1040, 1273
Hpy166II GTNNAC 6 cut(s) 175, 550, 844, 970, 1228, 1264
Hpy188III TCNNGA 9 cut(s) 21, 235, 326, 370, 536, 614, 766, 889, 950
Hpy8I GTNNAC 6 cut(s) 175, 550, 844, 970, 1228, 1264
Hpy99I CGWCG 5 cut(s) 266, 542, 715, 932, 1229
HpyAV CCTTC 2 cut(s) 362, 367
HpyCH4III ACNGT 3 cut(s) 798, 886, 1219
HpyCH4IV ACGT 2 cut(s) 723, 739
HpyCH4V TGCA 5 cut(s) 776, 839, 1024, 1112, 1202
HpyF3I CTNAG 8 cut(s) 169, 309, 388, 603, 936, 964, 1056, 1090
HpySE526I ACGT 2 cut(s) 723, 739
Hsp92II CATG 8 cut(s) 776, 839, 850, 892, 953, 1186, 1260, 1351
Kpn2I TCCGGA 1 cut(s) 369
Kzo9I GATC 8 cut(s) 6, 382, 391, 592, 707, 875, 891, 1173
LmnI GCTCC 1 cut(s) 1178
Lsp1109I GCAGC 3 cut(s) 324, 769, 907
LweI GCATC 3 cut(s) 67, 240, 667
MaeI CTAG 4 cut(s) 614, 756, 789, 1128
MaeII ACGT 2 cut(s) 723, 739
MaeIII GTNAC 1 cut(s) 959
MalI GATC 8 cut(s) 8, 384, 393, 594, 709, 877, 893, 1175
MboI GATC 8 cut(s) 6, 382, 391, 592, 707, 875, 891, 1173
MboII GAAGA 8 cut(s) 33, 36, 39, 76, 193, 353, 571, 1043
MfeI CAATTG 2 cut(s) 117, 295
MflI RGATCY 2 cut(s) 391, 592
MhlI GDGCHC 2 cut(s) 535, 796
MluCI AATT 5 cut(s) 117, 295, 649, 862, 975
MlyI GAGTC 1 cut(s) 300
Mph1103I ATGCAT 1 cut(s) 1114
MroI TCCGGA 1 cut(s) 369
MroXI GAANNNNTTC 3 cut(s) 723, 862, 1051
MseI TTAA 6 cut(s) 146, 318, 620, 639, 978, 1136
MslI CAYNNNNRTG 1 cut(s) 740
MspA1I CMGCKG 1 cut(s) 337
MspCI CTTAAG 1 cut(s) 317
MspI CCGG 4 cut(s) 225, 370, 804, 1100
MspR9I CCNGG 1 cut(s) 1335
MunI CAATTG 2 cut(s) 117, 295
Mva1269I GAATGC 1 cut(s) 1112
MvaI CCWGG 1 cut(s) 1335
MvnI CGCG 4 cut(s) 266, 528, 537, 856
NcoI CCATGG 1 cut(s) 1182
NdeII GATC 8 cut(s) 6, 382, 391, 592, 707, 875, 891, 1173
NheI GCTAGC 1 cut(s) 1127
NlaIII CATG 8 cut(s) 776, 839, 850, 892, 953, 1186, 1260, 1351
NlaIV GGNNCC 2 cut(s) 1048, 1180
NmuCI GTSAC 1 cut(s) 959
NruI TCGCGA 1 cut(s) 537
NsiI ATGCAT 1 cut(s) 1114
NspI RCATGY 2 cut(s) 776, 1351
OliI CACNNNNGTG 1 cut(s) 740
PaeR7I CTCGAG 1 cut(s) 288
PagI TCATGA 2 cut(s) 888, 949
PctI GAATGC 1 cut(s) 1112
PdmI GAANNNNTTC 3 cut(s) 723, 862, 1051
PfeI GAWTC 3 cut(s) 983, 996, 1041
PflMI CCANNNNNTGG 1 cut(s) 1334
PfoI TCCNGGA 1 cut(s) 1333
PkrI GCNGC 5 cut(s) 230, 339, 784, 922, 925
Ple19I CGATCG 1 cut(s) 878
PleI GAGTC 1 cut(s) 299
PmaCI CACGTG 1 cut(s) 740
PmlI CACGTG 1 cut(s) 740
PpsI GAGTC 1 cut(s) 299
Ppu21I YACGTR 1 cut(s) 740
Psp1406I AACGTT 1 cut(s) 723
Psp6I CCWGG 1 cut(s) 1333
PspCI CACGTG 1 cut(s) 740
PspFI CCCAGC 2 cut(s) 333, 1122
PspGI CCWGG 1 cut(s) 1333
PspN4I GGNNCC 2 cut(s) 1048, 1180
PspPI GGNCC 2 cut(s) 912, 1196
PspXI VCTCGAGB 1 cut(s) 288
PsrI GAACNNNNNNTAC 2 cut(s) 230, 262
PsuI RGATCY 2 cut(s) 391, 592
PvuI CGATCG 1 cut(s) 878
PvuII CAGCTG 1 cut(s) 337
RruI TCGCGA 1 cut(s) 537
RsaI GTAC 2 cut(s) 549, 808
RsaNI GTAC 2 cut(s) 548, 807
RseI CAYNNNNRTG 1 cut(s) 740
SalI GTCGAC 1 cut(s) 1226
SaqAI TTAA 6 cut(s) 146, 318, 620, 639, 978, 1136
SatI GCNGC 5 cut(s) 229, 338, 783, 921, 924
Sau3AI GATC 8 cut(s) 6, 382, 391, 592, 707, 875, 891, 1173
Sau96I GGNCC 2 cut(s) 912, 1196
SchI GAGTC 1 cut(s) 300
ScrFI CCNGG 1 cut(s) 1335
SduI GDGCHC 2 cut(s) 535, 796
SfaNI GCATC 3 cut(s) 67, 240, 667
Sfr274I CTCGAG 1 cut(s) 288
SinI GGWCC 1 cut(s) 1196
SlaI CTCGAG 1 cut(s) 288
SmiMI CAYNNNNRTG 1 cut(s) 740
SmlI CTYRAG 3 cut(s) 288, 317, 1073
SmoI CTYRAG 3 cut(s) 288, 317, 1073
SpeI ACTAGT 1 cut(s) 755
Sse9I AATT 5 cut(s) 117, 295, 649, 862, 975
SsiI CCGC 6 cut(s) 102, 229, 266, 528, 854, 924
SspMI CTAG 4 cut(s) 614, 756, 789, 1128
StyD4I CCNGG 1 cut(s) 1333
StyI CCWWGG 1 cut(s) 1182
TaaI ACNGT 3 cut(s) 798, 886, 1219
TaiI ACGT 2 cut(s) 726, 742
TaqI TCGA 8 cut(s) 87, 261, 282, 289, 477, 710, 999, 1227
TasI AATT 5 cut(s) 117, 295, 649, 862, 975
TatI WGTACW 1 cut(s) 547
TauI GCSGC 2 cut(s) 231, 926
TfiI GAWTC 3 cut(s) 983, 996, 1041
Tru1I TTAA 6 cut(s) 146, 318, 620, 639, 978, 1136
Tru9I TTAA 6 cut(s) 146, 318, 620, 639, 978, 1136
TscAI CASTG 4 cut(s) 702, 801, 846, 1222
TseFI GTSAC 1 cut(s) 959
TseI GCWGC 3 cut(s) 337, 782, 920
Tsp45I GTSAC 1 cut(s) 959
TspDTI ATGAA 2 cut(s) 824, 875
TspGWI ACGGA 2 cut(s) 448, 585
TspRI CASTG 4 cut(s) 702, 801, 846, 1222
Van91I CCANNNNNTGG 1 cut(s) 1334
Vha464I CTTAAG 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 1196
XapI RAATTY 1 cut(s) 862
XbaI TCTAGA 1 cut(s) 613
XceI RCATGY 2 cut(s) 776, 1351
XhoI CTCGAG 1 cut(s) 288
XmiI GTMKAC 1 cut(s) 1227
XmnI GAANNNNTTC 3 cut(s) 723, 862, 1051
XspI CTAG 4 cut(s) 614, 756, 789, 1128
Zsp2I ATGCAT 1 cut(s) 1114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.