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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 2 cut(s) | 14, 176 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 93 |
| AgsI | TTSAA | 2 cut(s) | 35, 47 |
| Alw26I | GTCTC | 1 cut(s) | 75 |
| ApoI | RAATTY | 2 cut(s) | 14, 176 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 80, 112 |
| AsuII | TTCGAA | 1 cut(s) | 12 |
| BccI | CCATC | 1 cut(s) | 54 |
| BcoDI | GTCTC | 1 cut(s) | 75 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 55, 87 |
| Bpu14I | TTCGAA | 1 cut(s) | 12 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 93 |
| Bse1I | ACTGG | 1 cut(s) | 177 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 93 |
| BseNI | ACTGG | 1 cut(s) | 177 |
| BslFI | GGGAC | 1 cut(s) | 160 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 93 |
| BsmAI | GTCTC | 1 cut(s) | 75 |
| BsmFI | GGGAC | 1 cut(s) | 160 |
| BsmI | GAATGC | 1 cut(s) | 115 |
| Bsp119I | TTCGAA | 1 cut(s) | 12 |
| BspT104I | TTCGAA | 1 cut(s) | 12 |
| BsrI | ACTGG | 1 cut(s) | 177 |
| BstBI | TTCGAA | 1 cut(s) | 12 |
| BstMAI | GTCTC | 1 cut(s) | 75 |
| CviAII | CATG | 1 cut(s) | 150 |
| FaeI | CATG | 1 cut(s) | 153 |
| FaiI | YATR | 3 cut(s) | 20, 76, 151 |
| FaqI | GGGAC | 1 cut(s) | 160 |
| FatI | CATG | 1 cut(s) | 149 |
| Hin1II | CATG | 1 cut(s) | 153 |
| HinfI | GANTC | 1 cut(s) | 9 |
| Hpy188I | TCNGA | 1 cut(s) | 8 |
| HpyAV | CCTTC | 1 cut(s) | 29 |
| Hsp92II | CATG | 1 cut(s) | 153 |
| LpnPI | CCDG | 1 cut(s) | 158 |
| MluCI | AATT | 2 cut(s) | 14, 176 |
| MnlI | CCTC | 1 cut(s) | 124 |
| MseI | TTAA | 1 cut(s) | 190 |
| Mva1269I | GAATGC | 1 cut(s) | 115 |
| NlaIII | CATG | 1 cut(s) | 153 |
| NspV | TTCGAA | 1 cut(s) | 12 |
| PctI | GAATGC | 1 cut(s) | 115 |
| PfeI | GAWTC | 1 cut(s) | 9 |
| SaqAI | TTAA | 1 cut(s) | 190 |
| SfuI | TTCGAA | 1 cut(s) | 12 |
| SgeI | CNNG | 4 cut(s) | 17, 155, 162, 185 |
| Sse9I | AATT | 2 cut(s) | 14, 176 |
| TaqI | TCGA | 2 cut(s) | 12, 58 |
| TasI | AATT | 2 cut(s) | 14, 176 |
| TfiI | GAWTC | 1 cut(s) | 9 |
| Tru1I | TTAA | 1 cut(s) | 190 |
| Tru9I | TTAA | 1 cut(s) | 190 |
| XapI | RAATTY | 2 cut(s) | 14, 176 |