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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcoI | YGGCCR | 2 cut(s) | 153, 174 |
| AcsI | RAATTY | 1 cut(s) | 26 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 70, 162, 188 |
| AjiI | CACGTC | 1 cut(s) | 209 |
| AjnI | CCWGG | 1 cut(s) | 160 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 176, 208 |
| AleI | CACNNNNGTG | 1 cut(s) | 211 |
| AluBI | AGCT | 1 cut(s) | 187 |
| AluI | AGCT | 1 cut(s) | 187 |
| Alw26I | GTCTC | 1 cut(s) | 83 |
| AoxI | GGCC | 3 cut(s) | 116, 153, 174 |
| ApoI | RAATTY | 1 cut(s) | 26 |
| AspS9I | GGNCC | 1 cut(s) | 117 |
| AsuC2I | CCSGG | 1 cut(s) | 71 |
| BaeGI | GKGCMC | 1 cut(s) | 168 |
| BciT130I | CCWGG | 1 cut(s) | 162 |
| BcnI | CCSGG | 1 cut(s) | 71 |
| BcoDI | GTCTC | 1 cut(s) | 83 |
| Bme1390I | CCNGG | 2 cut(s) | 71, 162 |
| BmgBI | CACGTC | 1 cut(s) | 209 |
| BmgT120I | GGNCC | 1 cut(s) | 117 |
| BmrFI | CCNGG | 2 cut(s) | 71, 162 |
| BpuMI | CCSGG | 1 cut(s) | 71 |
| BsaJI | CCNNGG | 2 cut(s) | 160, 161 |
| BsaWI | WCCGGW | 1 cut(s) | 135 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 90, 120 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 70, 162, 188 |
| BseBI | CCWGG | 1 cut(s) | 162 |
| BseDI | CCNNGG | 2 cut(s) | 160, 161 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 70, 162, 188 |
| BseRI | GAGGAG | 1 cut(s) | 138 |
| BseSI | GKGCMC | 1 cut(s) | 168 |
| BseX3I | CGGCCG | 1 cut(s) | 153 |
| Bsh1285I | CGRYCG | 1 cut(s) | 156 |
| BshFI | GGCC | 3 cut(s) | 118, 155, 176 |
| BsiEI | CGRYCG | 1 cut(s) | 156 |
| BsiSI | CCGG | 4 cut(s) | 70, 136, 152, 173 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 70, 162, 188 |
| BsmAI | GTCTC | 1 cut(s) | 83 |
| BsnI | GGCC | 3 cut(s) | 118, 155, 176 |
| Bsp1286I | GDGCHC | 1 cut(s) | 168 |
| Bsp143I | GATC | 1 cut(s) | 121 |
| BspANI | GGCC | 3 cut(s) | 118, 155, 176 |
| BssECI | CCNNGG | 2 cut(s) | 160, 161 |
| BssMI | GATC | 1 cut(s) | 121 |
| Bst2UI | CCWGG | 1 cut(s) | 162 |
| Bst4CI | ACNGT | 1 cut(s) | 34 |
| BstKTI | GATC | 1 cut(s) | 124 |
| BstMAI | GTCTC | 1 cut(s) | 83 |
| BstMBI | GATC | 1 cut(s) | 121 |
| BstMCI | CGRYCG | 1 cut(s) | 156 |
| BstNI | CCWGG | 1 cut(s) | 162 |
| BstSCI | CCNGG | 2 cut(s) | 69, 160 |
| BstSLI | GKGCMC | 1 cut(s) | 168 |
| BstZI | CGGCCG | 1 cut(s) | 153 |
| BsuRI | GGCC | 3 cut(s) | 118, 155, 176 |
| BtrI | CACGTC | 1 cut(s) | 209 |
| Cfr13I | GGNCC | 1 cut(s) | 117 |
| CviJI | RGCY | 4 cut(s) | 118, 155, 176, 187 |
| CviKI_1 | RGCY | 4 cut(s) | 118, 155, 176, 187 |
| DpnI | GATC | 1 cut(s) | 123 |
| DpnII | GATC | 1 cut(s) | 121 |
| EaeI | YGGCCR | 2 cut(s) | 153, 174 |
| EagI | CGGCCG | 1 cut(s) | 153 |
| EclXI | CGGCCG | 1 cut(s) | 153 |
| Eco52I | CGGCCG | 1 cut(s) | 153 |
| EcoRII | CCWGG | 1 cut(s) | 160 |
| FaiI | YATR | 3 cut(s) | 14, 16, 18 |
| HaeIII | GGCC | 3 cut(s) | 118, 155, 176 |
| HapII | CCGG | 4 cut(s) | 70, 136, 152, 173 |
| HpaII | CCGG | 4 cut(s) | 70, 136, 152, 173 |
| Hpy166II | GTNNAC | 1 cut(s) | 130 |
| Hpy188I | TCNGA | 1 cut(s) | 55 |
| Hpy8I | GTNNAC | 1 cut(s) | 130 |
| HpyCH4III | ACNGT | 1 cut(s) | 34 |
| HpyCH4IV | ACGT | 1 cut(s) | 208 |
| HpySE526I | ACGT | 1 cut(s) | 208 |
| Kzo9I | GATC | 1 cut(s) | 121 |
| LpnPI | CCDG | 6 cut(s) | 83, 147, 149, 165, 174, 186 |
| MaeII | ACGT | 1 cut(s) | 208 |
| MaeIII | GTNAC | 1 cut(s) | 204 |
| MalI | GATC | 1 cut(s) | 123 |
| MboI | GATC | 1 cut(s) | 121 |
| MboII | GAAGA | 1 cut(s) | 187 |
| MhlI | GDGCHC | 1 cut(s) | 168 |
| MluCI | AATT | 1 cut(s) | 26 |
| MnlI | CCTC | 5 cut(s) | 121, 143, 156, 159, 210 |
| MslI | CAYNNNNRTG | 1 cut(s) | 211 |
| MspI | CCGG | 4 cut(s) | 70, 136, 152, 173 |
| MspR9I | CCNGG | 2 cut(s) | 71, 162 |
| MvaI | CCWGG | 1 cut(s) | 162 |
| NciI | CCSGG | 1 cut(s) | 71 |
| NdeII | GATC | 1 cut(s) | 121 |
| NmeAIII | GCCGAG | 1 cut(s) | 94 |
| NmuCI | GTSAC | 1 cut(s) | 204 |
| OliI | CACNNNNGTG | 1 cut(s) | 211 |
| PasI | CCCWGGG | 1 cut(s) | 161 |
| Psp6I | CCWGG | 1 cut(s) | 160 |
| PspGI | CCWGG | 1 cut(s) | 160 |
| PspPI | GGNCC | 1 cut(s) | 117 |
| RseI | CAYNNNNRTG | 1 cut(s) | 211 |
| Sau3AI | GATC | 1 cut(s) | 121 |
| Sau96I | GGNCC | 1 cut(s) | 117 |
| ScrFI | CCNGG | 2 cut(s) | 71, 162 |
| SduI | GDGCHC | 1 cut(s) | 168 |
| SetI | ASST | 4 cut(s) | 135, 189, 211, 221 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 211 |
| Sse9I | AATT | 1 cut(s) | 26 |
| StyD4I | CCNGG | 2 cut(s) | 69, 160 |
| TaaI | ACNGT | 1 cut(s) | 34 |
| TaiI | ACGT | 1 cut(s) | 211 |
| TasI | AATT | 1 cut(s) | 26 |
| TseFI | GTSAC | 1 cut(s) | 204 |
| Tsp45I | GTSAC | 1 cut(s) | 204 |
| XapI | RAATTY | 1 cut(s) | 26 |