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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AfaI | GTAC | 2 cut(s) | 90, 124 |
| AluBI | AGCT | 1 cut(s) | 46 |
| AluI | AGCT | 1 cut(s) | 46 |
| BceAI | ACGGC | 1 cut(s) | 65 |
| BfaI | CTAG | 1 cut(s) | 53 |
| BmcAI | AGTACT | 1 cut(s) | 90 |
| BsaAI | YACGTR | 1 cut(s) | 126 |
| Bse3DI | GCAATG | 1 cut(s) | 41 |
| BseMI | GCAATG | 1 cut(s) | 41 |
| BsiWI | CGTACG | 1 cut(s) | 122 |
| BsrDI | GCAATG | 1 cut(s) | 41 |
| BstBAI | YACGTR | 1 cut(s) | 126 |
| BstC8I | GCNNGC | 2 cut(s) | 44, 99 |
| Cac8I | GCNNGC | 2 cut(s) | 44, 99 |
| Csp6I | GTAC | 2 cut(s) | 89, 123 |
| CviJI | RGCY | 2 cut(s) | 46, 52 |
| CviKI_1 | RGCY | 2 cut(s) | 46, 52 |
| CviQI | GTAC | 2 cut(s) | 89, 123 |
| FaiI | YATR | 1 cut(s) | 139 |
| FspBI | CTAG | 1 cut(s) | 53 |
| HpyCH4IV | ACGT | 1 cut(s) | 125 |
| HpyCH4V | TGCA | 1 cut(s) | 97 |
| HpySE526I | ACGT | 1 cut(s) | 125 |
| LpnPI | CCDG | 2 cut(s) | 4, 83 |
| MaeI | CTAG | 1 cut(s) | 53 |
| MaeII | ACGT | 1 cut(s) | 125 |
| MnlI | CCTC | 2 cut(s) | 106, 127 |
| Pfl23II | CGTACG | 1 cut(s) | 122 |
| Ppu21I | YACGTR | 1 cut(s) | 126 |
| PspLI | CGTACG | 1 cut(s) | 122 |
| RsaI | GTAC | 2 cut(s) | 90, 124 |
| RsaNI | GTAC | 2 cut(s) | 89, 123 |
| ScaI | AGTACT | 1 cut(s) | 90 |
| SetI | ASST | 4 cut(s) | 23, 48, 117, 128 |
| SgeI | CNNG | 6 cut(s) | 31, 55, 65, 78, 110, 130 |
| SspMI | CTAG | 1 cut(s) | 53 |
| TaiI | ACGT | 1 cut(s) | 128 |
| TaqI | TCGA | 1 cut(s) | 132 |
| TatI | WGTACW | 1 cut(s) | 88 |
| XspI | CTAG | 1 cut(s) | 53 |
| ZrmI | AGTACT | 1 cut(s) | 90 |