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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcoI | YGGCCR | 4 cut(s) | 86, 122, 143, 205 |
| AcsI | RAATTY | 1 cut(s) | 91 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 157 |
| AjiI | CACGTC | 1 cut(s) | 178 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 145, 177 |
| AleI | CACNNNNGTG | 1 cut(s) | 180 |
| AluBI | AGCT | 2 cut(s) | 156, 216 |
| AluI | AGCT | 2 cut(s) | 156, 216 |
| AoxI | GGCC | 4 cut(s) | 86, 122, 143, 205 |
| ApoI | RAATTY | 1 cut(s) | 91 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 95 |
| AsuC2I | CCSGG | 2 cut(s) | 41, 171 |
| BcnI | CCSGG | 2 cut(s) | 41, 171 |
| Bme1390I | CCNGG | 2 cut(s) | 41, 171 |
| BmgBI | CACGTC | 1 cut(s) | 178 |
| BmrFI | CCNGG | 2 cut(s) | 41, 171 |
| BpmI | CTGGAG | 1 cut(s) | 114 |
| BpuMI | CCSGG | 2 cut(s) | 41, 171 |
| BsaJI | CCNNGG | 1 cut(s) | 169 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 60, 90 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 157 |
| BseDI | CCNNGG | 1 cut(s) | 169 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 157 |
| BseX3I | CGGCCG | 2 cut(s) | 86, 122 |
| Bsh1285I | CGRYCG | 2 cut(s) | 89, 125 |
| BshFI | GGCC | 4 cut(s) | 88, 124, 145, 207 |
| BsiEI | CGRYCG | 2 cut(s) | 89, 125 |
| BsiSI | CCGG | 5 cut(s) | 40, 121, 142, 171, 208 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 157 |
| BsnI | GGCC | 4 cut(s) | 88, 124, 145, 207 |
| BspANI | GGCC | 4 cut(s) | 88, 124, 145, 207 |
| BssECI | CCNNGG | 1 cut(s) | 169 |
| Bst6I | CTCTTC | 3 cut(s) | 54, 77, 122 |
| BstMCI | CGRYCG | 2 cut(s) | 89, 125 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 213 |
| BstSCI | CCNGG | 2 cut(s) | 39, 169 |
| BstZI | CGGCCG | 2 cut(s) | 86, 122 |
| BsuRI | GGCC | 4 cut(s) | 88, 124, 145, 207 |
| BtrI | CACGTC | 1 cut(s) | 178 |
| CviJI | RGCY | 7 cut(s) | 5, 88, 124, 145, 156, 207, 216 |
| CviKI_1 | RGCY | 7 cut(s) | 5, 88, 124, 145, 156, 207, 216 |
| EaeI | YGGCCR | 4 cut(s) | 86, 122, 143, 205 |
| EagI | CGGCCG | 2 cut(s) | 86, 122 |
| Eam1104I | CTCTTC | 3 cut(s) | 54, 77, 122 |
| EarI | CTCTTC | 3 cut(s) | 54, 77, 122 |
| EclXI | CGGCCG | 2 cut(s) | 86, 122 |
| Eco52I | CGGCCG | 2 cut(s) | 86, 122 |
| EcoRI | GAATTC | 1 cut(s) | 91 |
| GsuI | CTGGAG | 1 cut(s) | 114 |
| HaeIII | GGCC | 4 cut(s) | 88, 124, 145, 207 |
| HapII | CCGG | 5 cut(s) | 40, 121, 142, 171, 208 |
| HinfI | GANTC | 2 cut(s) | 69, 127 |
| HpaII | CCGG | 5 cut(s) | 40, 121, 142, 171, 208 |
| Hpy188I | TCNGA | 1 cut(s) | 220 |
| Hpy188III | TCNNGA | 1 cut(s) | 131 |
| HpyAV | CCTTC | 2 cut(s) | 106, 112 |
| HpyCH4IV | ACGT | 2 cut(s) | 135, 177 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 213 |
| HpySE526I | ACGT | 2 cut(s) | 135, 177 |
| LpnPI | CCDG | 6 cut(s) | 53, 134, 144, 155, 184, 221 |
| MaeII | ACGT | 2 cut(s) | 135, 177 |
| MaeIII | GTNAC | 2 cut(s) | 43, 173 |
| MboII | GAAGA | 5 cut(s) | 41, 64, 103, 109, 156 |
| MluCI | AATT | 1 cut(s) | 91 |
| MlyI | GAGTC | 2 cut(s) | 63, 121 |
| MnlI | CCTC | 4 cut(s) | 86, 91, 125, 179 |
| MroXI | GAANNNNTTC | 1 cut(s) | 95 |
| MslI | CAYNNNNRTG | 1 cut(s) | 180 |
| MspI | CCGG | 5 cut(s) | 40, 121, 142, 171, 208 |
| MspR9I | CCNGG | 2 cut(s) | 41, 171 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 213 |
| NciI | CCSGG | 2 cut(s) | 41, 171 |
| NmeAIII | GCCGAG | 1 cut(s) | 64 |
| NmuCI | GTSAC | 1 cut(s) | 173 |
| OliI | CACNNNNGTG | 1 cut(s) | 180 |
| PdmI | GAANNNNTTC | 1 cut(s) | 95 |
| PleI | GAGTC | 2 cut(s) | 63, 121 |
| PpsI | GAGTC | 2 cut(s) | 63, 121 |
| RseI | CAYNNNNRTG | 1 cut(s) | 180 |
| SchI | GAGTC | 2 cut(s) | 63, 121 |
| ScrFI | CCNGG | 2 cut(s) | 41, 171 |
| SetI | ASST | 6 cut(s) | 33, 138, 158, 180, 190, 218 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 180 |
| Sse9I | AATT | 1 cut(s) | 91 |
| SspI | AATATT | 1 cut(s) | 13 |
| StyD4I | CCNGG | 2 cut(s) | 39, 169 |
| TaiI | ACGT | 2 cut(s) | 138, 180 |
| TaqI | TCGA | 1 cut(s) | 105 |
| TasI | AATT | 1 cut(s) | 91 |
| TseFI | GTSAC | 1 cut(s) | 173 |
| Tsp45I | GTSAC | 1 cut(s) | 173 |
| XapI | RAATTY | 1 cut(s) | 91 |
| XmnI | GAANNNNTTC | 1 cut(s) | 95 |