RchiOBHm_Chr2g0122881

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0003698
Physical Location & Seq
Forward (+)
35988917 .. 35990569
1653 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49524

Sequence Viewer

Length: 1020 bp
ATGGAAGAGCAAGAAGAGAGAGTGAAGGGAAGCAGTAAGAGGGATAGACTAATCGAAGAGTCGAAACCCTACATCCTCTGTATATTTTCTAATGCATGCTTTTCAGGGTACAATATTGTCTCCAAAGTCGCTCTAGACAAGGGCATGAGCCGTTATGTGCTTGTGGTTTATGGACATGCTTTTGGAACTCTAGCAACTGCTCTTTTTGCATTTCTCTTTGAGAGTGGAGTGTTAGGAAGAACACTATACTACATGGGATTGGAATACACCTCACCAGCTTTTGCATCTGCCATGGGCAACATGATTCCATCGATTACCTTCATCTTAGCTGTTTTGTGCAGGATGGAACAGTTCGATATTTCGAAGCTTCGTACCCAAGCCAAGATTGGGGGAACTCTTGCTGCCTTTGCTGGTGCGACACTCATGACCCTCTATAAGGGTGTTGCTGTAATTACAACGCACACCAAAAGCTCCCATCAAACTGCAGCTACCTCAAAATCATCTACAGACGCAGAATGGATAAAGGGCTCCCTTGTCCTTATTGTTTCATTTAGTTCCTTAGCAGCATTCTATATCTTACAGACATCAACAATTAAAATGTATCCAGCACCAATCACTCTTACATCATTGACCTGCTTATCTGGGACACTGCTCTCAACAATCATGGCAGCAATTCTAGATCACAAAGCATCTTCTTGGAAGTTATCATGGAACACACTTCTTGCTCCTATGTATAGTGGAATTGTGATTTTCGGAATCACAGTTTATGTTCAGACCTTAGTAGTACGAACAAAAGGCCCAGTTTTCATGACAGCTTTTAGACCACTAGCTACCATTGTTGTAGCTATAATGGGACTACTCATTTTAGGAGAAGCAATATACTTGGGAAGTGTTTTGGGAGCTTTCTTGATAGTTCTTGGTCTGTACGCAACTCTTTGGGGAAAGAAAAAAGAGAAGGAGAAGAAGTCAATGGATCATGGTGTATCTCAGCAAGCTATTGAGATCAATCTAGAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

339

Amino Acids

36.9

Weight (kDa)

9.32

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 175 - 312 2.6e-07 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 641
AclWI GGATC 1 cut(s) 981
AfaI GTAC 4 cut(s) 110, 373, 786, 926
AfiI CCNNNNNNNGG 2 cut(s) 387, 436
Alw26I GTCTC 1 cut(s) 124
AlwI GGATC 1 cut(s) 981
AoxI GGCC 1 cut(s) 796
ApeKI GCWGC 4 cut(s) 401, 485, 563, 668
AspS9I GGNCC 1 cut(s) 797
AsuHPI GGTGA 1 cut(s) 264
AsuII TTCGAA 1 cut(s) 362
BanII GRGCYC 1 cut(s) 530
BbvI GCAGC 4 cut(s) 388, 497, 575, 680
BccI CCATC 3 cut(s) 316, 337, 483
BceAI ACGGC 1 cut(s) 135
BciVI GTATCC 1 cut(s) 612
BcoDI GTCTC 1 cut(s) 124
BfaI CTAG 5 cut(s) 134, 191, 677, 827, 1010
BfmI CTRYAG 2 cut(s) 483, 504
BfuAI ACCTGC 1 cut(s) 641
BfuI GTATCC 1 cut(s) 612
BisI GCNGC 4 cut(s) 402, 486, 564, 669
BlsI GCNGC 4 cut(s) 403, 487, 565, 670
BmgT120I GGNCC 1 cut(s) 797
BmiI GGNNCC 1 cut(s) 529
BmrI ACTGGG 1 cut(s) 794
BmsI GCATC 2 cut(s) 293, 698
BmuI ACTGGG 1 cut(s) 794
Bpu10I CCTNAGC 1 cut(s) 559
Bpu14I TTCGAA 1 cut(s) 362
Bsa29I ATCGAT 1 cut(s) 311
BsaJI CCNNGG 1 cut(s) 291
BsaXI ACNNNNNCTCC 2 cut(s) 950, 980
Bsc4I CCNNNNNNNGG 2 cut(s) 387, 436
Bse1I ACTGG 1 cut(s) 800
BseCI ATCGAT 1 cut(s) 311
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 2 cut(s) 72, 348
BseLI CCNNNNNNNGG 2 cut(s) 387, 436
BseMII CTCAG 1 cut(s) 1001
BseNI ACTGG 1 cut(s) 800
BseXI GCAGC 4 cut(s) 388, 497, 575, 680
BsgI GTGCAG 1 cut(s) 358
BshFI GGCC 1 cut(s) 798
BshVI ATCGAT 1 cut(s) 311
BslFI GGGAC 2 cut(s) 658, 867
BslI CCNNNNNNNGG 2 cut(s) 387, 436
BsmAI GTCTC 1 cut(s) 124
BsmFI GGGAC 2 cut(s) 658, 867
BsmI GAATGC 1 cut(s) 566
BsnI GGCC 1 cut(s) 798
Bsp119I TTCGAA 1 cut(s) 362
Bsp1286I GDGCHC 1 cut(s) 530
Bsp143I GATC 3 cut(s) 679, 973, 1002
Bsp19I CCATGG 1 cut(s) 291
BspANI GGCC 1 cut(s) 798
BspCNI CTCAG 1 cut(s) 1000
BspDI ATCGAT 1 cut(s) 311
BspHI TCATGA 2 cut(s) 423, 807
BspLI GGNNCC 1 cut(s) 529
BspMAI CTGCAG 1 cut(s) 487
BspMI ACCTGC 1 cut(s) 641
BspPI GGATC 1 cut(s) 981
BspT104I TTCGAA 1 cut(s) 362
BsrI ACTGG 1 cut(s) 800
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 3 cut(s) 679, 973, 1002
BssT1I CCWWGG 1 cut(s) 291
Bst4CI ACNGT 2 cut(s) 351, 763
Bst6I CTCTTC 2 cut(s) 9, 51
BstBI TTCGAA 1 cut(s) 362
BstC8I GCNNGC 2 cut(s) 97, 993
BstDEI CTNAG 4 cut(s) 325, 559, 778, 987
BstDSI CCRYGG 1 cut(s) 291
BstENI CCTNNNNNAGG 1 cut(s) 434
BstF5I GGATG 2 cut(s) 72, 348
BstKTI GATC 3 cut(s) 682, 976, 1005
BstMAI GTCTC 1 cut(s) 124
BstMBI GATC 3 cut(s) 679, 973, 1002
BstMWI GCNNNNNNNGC 2 cut(s) 206, 407
BstNSI RCATGY 2 cut(s) 99, 179
BstSFI CTRYAG 2 cut(s) 483, 504
BstV1I GCAGC 4 cut(s) 388, 497, 575, 680
Bsu15I ATCGAT 1 cut(s) 311
BsuI GTATCC 1 cut(s) 612
BsuRI GGCC 1 cut(s) 798
BsuTUI ATCGAT 1 cut(s) 311
BtgI CCRYGG 1 cut(s) 291
BtsCI GGATG 2 cut(s) 72, 348
BtsI GCAGTG 1 cut(s) 647
BtsIMutI CAGTG 1 cut(s) 647
BveI ACCTGC 1 cut(s) 641
Cac8I GCNNGC 2 cut(s) 97, 993
CciI TCATGA 2 cut(s) 423, 807
Cfr13I GGNCC 1 cut(s) 797
ClaI ATCGAT 1 cut(s) 311
CseI GACGC 1 cut(s) 518
Csp6I GTAC 4 cut(s) 109, 372, 785, 925
CviQI GTAC 4 cut(s) 109, 372, 785, 925
DdeI CTNAG 4 cut(s) 325, 559, 778, 987
DpnI GATC 3 cut(s) 681, 975, 1004
DpnII GATC 3 cut(s) 679, 973, 1002
Eam1104I CTCTTC 2 cut(s) 9, 51
EarI CTCTTC 2 cut(s) 9, 51
Eco130I CCWWGG 1 cut(s) 291
Eco24I GRGCYC 1 cut(s) 530
EcoNI CCTNNNNNAGG 1 cut(s) 434
EcoT14I CCWWGG 1 cut(s) 291
EcoT22I ATGCAT 1 cut(s) 97
EcoT38I GRGCYC 1 cut(s) 530
ErhI CCWWGG 1 cut(s) 291
FaqI GGGAC 2 cut(s) 658, 867
Fnu4HI GCNGC 4 cut(s) 402, 486, 564, 669
FokI GGATG 2 cut(s) 59, 355
FriOI GRGCYC 1 cut(s) 530
Fsp4HI GCNGC 4 cut(s) 402, 486, 564, 669
FspBI CTAG 5 cut(s) 134, 191, 677, 827, 1010
GluI GCNGC 4 cut(s) 402, 486, 564, 669
HaeIII GGCC 1 cut(s) 798
HgaI GACGC 1 cut(s) 518
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 3 cut(s) 59, 304, 756
HphI GGTGA 1 cut(s) 264
Hpy188I TCNGA 2 cut(s) 755, 774
Hpy188III TCNNGA 6 cut(s) 134, 424, 677, 808, 907, 1010
HpyAV CCTTC 3 cut(s) 19, 328, 949
HpyCH4III ACNGT 2 cut(s) 351, 763
HpyCH4V TGCA 5 cut(s) 95, 209, 284, 339, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 407
HpyF3I CTNAG 4 cut(s) 325, 559, 778, 987
Kzo9I GATC 3 cut(s) 679, 973, 1002
LmnI GCTCC 4 cut(s) 476, 533, 730, 899
LpnPI CCDG 8 cut(s) 90, 288, 325, 396, 618, 627, 646, 813
Lsp1109I GCAGC 4 cut(s) 388, 497, 575, 680
LweI GCATC 2 cut(s) 293, 698
MaeI CTAG 5 cut(s) 134, 191, 677, 827, 1010
MalI GATC 3 cut(s) 681, 975, 1004
MboI GATC 3 cut(s) 679, 973, 1002
MboII GAAGA 6 cut(s) 17, 26, 68, 249, 684, 973
MhlI GDGCHC 1 cut(s) 530
MluCI AATT 4 cut(s) 450, 591, 672, 741
MlyI GAGTC 1 cut(s) 68
MnlI CCTC 5 cut(s) 33, 86, 280, 440, 502
Mph1103I ATGCAT 1 cut(s) 97
MseI TTAA 1 cut(s) 594
Mva1269I GAATGC 1 cut(s) 566
MwoI GCNNNNNNNGC 2 cut(s) 206, 407
NcoI CCATGG 1 cut(s) 291
NdeII GATC 3 cut(s) 679, 973, 1002
NlaIV GGNNCC 1 cut(s) 529
NsiI ATGCAT 1 cut(s) 97
NspI RCATGY 2 cut(s) 99, 179
NspV TTCGAA 1 cut(s) 362
PaeI GCATGC 1 cut(s) 99
PagI TCATGA 2 cut(s) 423, 807
PctI GAATGC 1 cut(s) 566
PfeI GAWTC 2 cut(s) 304, 756
PkrI GCNGC 4 cut(s) 403, 487, 565, 670
PleI GAGTC 1 cut(s) 67
PpsI GAGTC 1 cut(s) 67
PspN4I GGNNCC 1 cut(s) 529
PspPI GGNCC 1 cut(s) 797
PstI CTGCAG 1 cut(s) 487
RsaI GTAC 4 cut(s) 110, 373, 786, 926
RsaNI GTAC 4 cut(s) 109, 372, 785, 925
SaqAI TTAA 1 cut(s) 594
SatI GCNGC 4 cut(s) 402, 486, 564, 669
Sau3AI GATC 3 cut(s) 679, 973, 1002
Sau96I GGNCC 1 cut(s) 797
SchI GAGTC 1 cut(s) 68
SduI GDGCHC 1 cut(s) 530
SfaNI GCATC 2 cut(s) 293, 698
SfcI CTRYAG 2 cut(s) 483, 504
SfuI TTCGAA 1 cut(s) 362
SphI GCATGC 1 cut(s) 99
Sse9I AATT 4 cut(s) 450, 591, 672, 741
SspI AATATT 1 cut(s) 115
SspMI CTAG 5 cut(s) 134, 191, 677, 827, 1010
StyI CCWWGG 1 cut(s) 291
TaaI ACNGT 2 cut(s) 351, 763
TaqI TCGA 5 cut(s) 54, 62, 311, 354, 362
TasI AATT 4 cut(s) 450, 591, 672, 741
TfiI GAWTC 2 cut(s) 304, 756
Tru1I TTAA 1 cut(s) 594
Tru9I TTAA 1 cut(s) 594
TscAI CASTG 1 cut(s) 654
TseI GCWGC 4 cut(s) 401, 485, 563, 668
TspDTI ATGAA 3 cut(s) 310, 537, 796
TspRI CASTG 1 cut(s) 654
XagI CCTNNNNNAGG 1 cut(s) 434
XbaI TCTAGA 3 cut(s) 133, 676, 1009
XceI RCATGY 2 cut(s) 99, 179
XcmI CCANNNNNNNNNTGG 1 cut(s) 383
XspI CTAG 5 cut(s) 134, 191, 677, 827, 1010
Zsp2I ATGCAT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.