Rh2AG499200

EamA-like transporter family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0003698
Physical Location & Seq
Reverse (-)
72191039 .. 72192368
1330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG499200.1

Sequence Viewer

Length: 879 bp
ATGCAAAATGTAATGCAGATGGAAGAGCAAGAAGAGAGAGTTAAAGGAAGCCGTAAGTGGGATAGAGTAATCGAAGAATCGAAACCCTACATCCTCTGTATATTTTCTAATGTATGCTTTGCCGGGTACAATATTGTCTCCAAAGTCGCTCTACACAAAGGCATGAGCAGATATGTGCTTGTGGTTTATGGATATGCGTTTGGAACTCTAGCAACTGCTCTTTTTGCATTTCTCTTTGAGAGGAAAAATGAGAGCAAAATCAATGTTCAGATCTTGCGAAATGTCTTTTTCTTAGGTCTGCTGGGAGCGTTAGGAAGTACACTGTACTACATGGGATTGGAATATACTTCACCAGCTTTTACATCTGCCATGGCCAACATGACTCCATCGATTACCTTCATCTTAGCTGTTTTGTGCAGGATGGAACAGTTTGATATTTCCAAGCCTGGTACCCAAGCCAAGATTGGAGGAACTCTTGCTGCCTTTGCTGGTGCAACACTCATGACCCTCTACAAGGGCGTTGCTGTAATAACAATGCACACCAAAAGCTCCCATCAAACTGCAGGTACCTCAAAATCATATACAGACGCAGAATGGTTGAAGGGCTCCCTTGTCCTTATTGTTTCATATAGTTCATTTGCAGCATCCTATATCTTACAGACATCAACAATAAAAATGTATCCAGCACCAATCACTCTTACATCATTGACCTGCTTATCTGGGACACTGCTCTCAACAATCATGGCAGCAATTCTAGATCACAAAGCATCTTCTTGGAAGTTATCATGGAACACACTTCTTGCTCCTATGTATTGTGTAAGAACTCGATCACACCTCTTCAACTGTCTAAGGCCTATGATGTTTGTAAATGAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

292

Amino Acids

32.45

Weight (kDa)

9.36

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 29 - 168 2e-14 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 554, 719
Acc65I GGTACC 2 cut(s) 449, 566
AccB1I GGYRCC 2 cut(s) 449, 566
AcoI YGGCCR 1 cut(s) 372
AfaI GTAC 5 cut(s) 128, 319, 326, 451, 568
AfiI CCNNNNNNNGG 2 cut(s) 58, 514
AgsI TTSAA 2 cut(s) 601, 841
AjnI CCWGG 1 cut(s) 445
AluBI AGCT 3 cut(s) 356, 407, 549
AluI AGCT 3 cut(s) 356, 407, 549
Alw26I GTCTC 1 cut(s) 142
AoxI GGCC 2 cut(s) 372, 851
ApeKI GCWGC 3 cut(s) 479, 641, 746
Asp718I GGTACC 2 cut(s) 449, 566
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 1 cut(s) 342
BalI TGGCCA 1 cut(s) 374
BanI GGYRCC 2 cut(s) 449, 566
BanII GRGCYC 1 cut(s) 608
BbvI GCAGC 3 cut(s) 466, 653, 758
BccI CCATC 4 cut(s) 13, 394, 415, 561
BceAI ACGGC 1 cut(s) 36
BciT130I CCWGG 1 cut(s) 447
BciVI GTATCC 1 cut(s) 690
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 1 cut(s) 142
BfaI CTAG 2 cut(s) 209, 755
BfmI CTRYAG 1 cut(s) 561
BfuAI ACCTGC 2 cut(s) 554, 719
BfuI GTATCC 1 cut(s) 690
BglII AGATCT 1 cut(s) 270
BisI GCNGC 3 cut(s) 480, 642, 747
BlsI GCNGC 3 cut(s) 481, 643, 748
Bme1390I CCNGG 2 cut(s) 124, 447
BmiI GGNNCC 3 cut(s) 451, 568, 607
BmrFI CCNGG 2 cut(s) 124, 447
BmsI GCATC 2 cut(s) 653, 776
BpuMI CCSGG 1 cut(s) 124
Bsa29I ATCGAT 1 cut(s) 389
BsaJI CCNNGG 1 cut(s) 369
Bsc4I CCNNNNNNNGG 2 cut(s) 58, 514
BseBI CCWGG 1 cut(s) 447
BseCI ATCGAT 1 cut(s) 389
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 3 cut(s) 90, 426, 644
BseLI CCNNNNNNNGG 2 cut(s) 58, 514
BseXI GCAGC 3 cut(s) 466, 653, 758
BseYI CCCAGC 1 cut(s) 301
BsgI GTGCAG 1 cut(s) 436
BshFI GGCC 2 cut(s) 374, 853
BshNI GGYRCC 2 cut(s) 449, 566
BshVI ATCGAT 1 cut(s) 389
BsiSI CCGG 1 cut(s) 123
BslFI GGGAC 1 cut(s) 736
BslI CCNNNNNNNGG 2 cut(s) 58, 514
BsmAI GTCTC 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 736
BsnI GGCC 2 cut(s) 374, 853
Bsp1286I GDGCHC 1 cut(s) 608
Bsp143I GATC 3 cut(s) 270, 757, 827
Bsp19I CCATGG 1 cut(s) 369
BspANI GGCC 2 cut(s) 374, 853
BspDI ATCGAT 1 cut(s) 389
BspHI TCATGA 1 cut(s) 501
BspLI GGNNCC 3 cut(s) 451, 568, 607
BspMAI CTGCAG 1 cut(s) 565
BspMI ACCTGC 2 cut(s) 554, 719
BspQI GCTCTTC 1 cut(s) 18
BspT107I GGYRCC 2 cut(s) 449, 566
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 3 cut(s) 270, 757, 827
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 447
Bst4CI ACNGT 3 cut(s) 324, 429, 845
Bst6I CTCTTC 3 cut(s) 18, 27, 842
BstDEI CTNAG 3 cut(s) 292, 403, 848
BstDSI CCRYGG 1 cut(s) 369
BstENI CCTNNNNNAGG 1 cut(s) 512
BstF5I GGATG 3 cut(s) 90, 426, 644
BstKTI GATC 3 cut(s) 273, 760, 830
BstMAI GTCTC 1 cut(s) 142
BstMBI GATC 3 cut(s) 270, 757, 827
BstMWI GCNNNNNNNGC 2 cut(s) 224, 485
BstNI CCWGG 1 cut(s) 447
BstSCI CCNGG 2 cut(s) 122, 445
BstSFI CTRYAG 1 cut(s) 561
BstV1I GCAGC 3 cut(s) 466, 653, 758
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
Bsu15I ATCGAT 1 cut(s) 389
BsuI GTATCC 1 cut(s) 690
BsuRI GGCC 2 cut(s) 374, 853
BsuTUI ATCGAT 1 cut(s) 389
BtgI CCRYGG 1 cut(s) 369
BtsCI GGATG 3 cut(s) 90, 426, 644
BtsI GCAGTG 1 cut(s) 725
BtsIMutI CAGTG 2 cut(s) 320, 725
BveI ACCTGC 2 cut(s) 554, 719
CciI TCATGA 1 cut(s) 501
ClaI ATCGAT 1 cut(s) 389
CseI GACGC 1 cut(s) 596
Csp6I GTAC 5 cut(s) 127, 318, 325, 450, 567
CviAII CATG 7 cut(s) 163, 331, 370, 379, 502, 742, 786
CviJI RGCY 9 cut(s) 51, 356, 374, 407, 445, 458, 549, 606, 853
CviKI_1 RGCY 9 cut(s) 51, 356, 374, 407, 445, 458, 549, 606, 853
CviQI GTAC 5 cut(s) 127, 318, 325, 450, 567
DdeI CTNAG 3 cut(s) 292, 403, 848
DpnI GATC 3 cut(s) 272, 759, 829
DpnII GATC 3 cut(s) 270, 757, 827
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 3 cut(s) 18, 27, 842
EarI CTCTTC 3 cut(s) 18, 27, 842
Eco130I CCWWGG 1 cut(s) 369
Eco147I AGGCCT 1 cut(s) 853
Eco24I GRGCYC 1 cut(s) 608
EcoNI CCTNNNNNAGG 1 cut(s) 512
EcoRII CCWGG 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 369
EcoT38I GRGCYC 1 cut(s) 608
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 7 cut(s) 166, 334, 373, 382, 505, 745, 789
FaqI GGGAC 1 cut(s) 736
FatI CATG 7 cut(s) 162, 330, 369, 378, 501, 741, 785
Fnu4HI GCNGC 3 cut(s) 480, 642, 747
FokI GGATG 3 cut(s) 77, 433, 631
FriOI GRGCYC 1 cut(s) 608
Fsp4HI GCNGC 3 cut(s) 480, 642, 747
FspBI CTAG 2 cut(s) 209, 755
GluI GCNGC 3 cut(s) 480, 642, 747
GsaI CCCAGC 1 cut(s) 305
HaeIII GGCC 2 cut(s) 374, 853
HapII CCGG 1 cut(s) 123
HgaI GACGC 1 cut(s) 596
Hin1II CATG 7 cut(s) 166, 334, 373, 382, 505, 745, 789
HinfI GANTC 2 cut(s) 77, 382
HpaII CCGG 1 cut(s) 123
HphI GGTGA 1 cut(s) 342
Hpy166II GTNNAC 1 cut(s) 320
Hpy188I TCNGA 1 cut(s) 270
Hpy188III TCNNGA 2 cut(s) 502, 755
Hpy8I GTNNAC 1 cut(s) 320
HpyAV CCTTC 2 cut(s) 406, 595
HpyCH4III ACNGT 3 cut(s) 324, 429, 845
HpyCH4V TGCA 8 cut(s) 4, 16, 227, 417, 494, 538, 563, 641
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 485
HpyF3I CTNAG 3 cut(s) 292, 403, 848
Hsp92II CATG 7 cut(s) 166, 334, 373, 382, 505, 745, 789
KpnI GGTACC 2 cut(s) 453, 570
Kzo9I GATC 3 cut(s) 270, 757, 827
LguI GCTCTTC 1 cut(s) 18
LmnI GCTCC 4 cut(s) 305, 554, 611, 808
Lsp1109I GCAGC 3 cut(s) 466, 653, 758
LweI GCATC 2 cut(s) 653, 776
MaeI CTAG 2 cut(s) 209, 755
MalI GATC 3 cut(s) 272, 759, 829
MboI GATC 3 cut(s) 270, 757, 827
MboII GAAGA 5 cut(s) 35, 44, 86, 762, 829
MflI RGATCY 1 cut(s) 270
MhlI GDGCHC 1 cut(s) 608
MlsI TGGCCA 1 cut(s) 374
MluCI AATT 1 cut(s) 750
MluNI TGGCCA 1 cut(s) 374
MlyI GAGTC 1 cut(s) 376
MnlI CCTC 6 cut(s) 104, 234, 461, 518, 580, 845
Mox20I TGGCCA 1 cut(s) 374
MscI TGGCCA 1 cut(s) 374
MseI TTAA 1 cut(s) 42
Msp20I TGGCCA 1 cut(s) 374
MspI CCGG 1 cut(s) 123
MspR9I CCNGG 2 cut(s) 124, 447
MvaI CCWGG 1 cut(s) 447
MwoI GCNNNNNNNGC 2 cut(s) 224, 485
NciI CCSGG 1 cut(s) 124
NcoI CCATGG 1 cut(s) 369
NdeII GATC 3 cut(s) 270, 757, 827
NlaIII CATG 7 cut(s) 166, 334, 373, 382, 505, 745, 789
NlaIV GGNNCC 3 cut(s) 451, 568, 607
PagI TCATGA 1 cut(s) 501
PceI AGGCCT 1 cut(s) 853
PciSI GCTCTTC 1 cut(s) 18
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 3 cut(s) 481, 643, 748
PleI GAGTC 1 cut(s) 376
PpsI GAGTC 1 cut(s) 376
Psp6I CCWGG 1 cut(s) 445
PspFI CCCAGC 1 cut(s) 301
PspGI CCWGG 1 cut(s) 445
PspN4I GGNNCC 3 cut(s) 451, 568, 607
PstI CTGCAG 1 cut(s) 565
PsuI RGATCY 1 cut(s) 270
RsaI GTAC 5 cut(s) 128, 319, 326, 451, 568
RsaNI GTAC 5 cut(s) 127, 318, 325, 450, 567
SapI GCTCTTC 1 cut(s) 18
SaqAI TTAA 1 cut(s) 42
SatI GCNGC 3 cut(s) 480, 642, 747
Sau3AI GATC 3 cut(s) 270, 757, 827
SchI GAGTC 1 cut(s) 376
ScrFI CCNGG 2 cut(s) 124, 447
SduI GDGCHC 1 cut(s) 608
SetI ASST 9 cut(s) 298, 358, 398, 409, 551, 568, 572, 713, 837
SfaNI GCATC 2 cut(s) 653, 776
SfcI CTRYAG 1 cut(s) 561
Sse9I AATT 1 cut(s) 750
SseBI AGGCCT 1 cut(s) 853
SspI AATATT 1 cut(s) 133
SspMI CTAG 2 cut(s) 209, 755
StuI AGGCCT 1 cut(s) 853
StyD4I CCNGG 2 cut(s) 122, 445
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 3 cut(s) 324, 429, 845
TaqI TCGA 4 cut(s) 72, 80, 389, 826
TasI AATT 1 cut(s) 750
TatI WGTACW 2 cut(s) 317, 324
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 1 cut(s) 42
Tru9I TTAA 1 cut(s) 42
TscAI CASTG 2 cut(s) 327, 732
TseI GCWGC 3 cut(s) 479, 641, 746
TspDTI ATGAA 3 cut(s) 388, 615, 624
TspRI CASTG 2 cut(s) 327, 732
XagI CCTNNNNNAGG 1 cut(s) 512
XbaI TCTAGA 1 cut(s) 754
XcmI CCANNNNNNNNNTGG 1 cut(s) 461
XspI CTAG 2 cut(s) 209, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.